KR102247944B1 - Antioxidant and whitening composition - Google Patents

Abstract

Most of the existing skin whitening compositions are substances applied to the skin. However, the time of use of the whitening composition applied to the skin was limited, and the effect was unclear. In order to solve this problem, the antioxidant and whitening composition of the present invention is glutathione containing 5% by weight of plum, 50% by weight of xylitol, 29.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 50% by weight of glutathione. It provides an antioxidant and whitening composition, characterized in that 0.5% by weight of yeast.
By providing a food composition using the antioxidant function of plum and the active oxygen removal function of glutathione yeast by the above configuration, a composition capable of living a healthy life is provided.

Description

Antioxidant and whitening composition{.}

The present invention relates to a food composition having antioxidant and whitening functions, and more particularly, to a plum extract containing Glutathione Yeast.

Prior art prior to the present invention includes a cosmetic composition for whitening, a food composition for whitening, a health functional food for the prevention or alleviation of pigmentation diseases containing the composition, and for the prevention or treatment of pigmentation diseases Description of the pharmaceutical composition is disclosed.

Another prior art relates to an antioxidant and whitening cosmetic composition containing extracts of nogeun, green tea and plum, and an ethyl acetate-extracted fraction for the ethanol-extracted freeze-dried product of Nogeun, and ethyl acetate or butanol for the freeze-dried product of ethanol-extracted green tea A composition comprising a mixture of an extracted fraction and a freeze-dried product of ethanol extraction of plum is disclosed.

Registered Patent Publication 10-1768626 Registered Patent Publication 10-1704874

Most of the existing skin whitening compositions are substances applied to the skin. However, the time of use of the whitening composition applied to the skin was limited, and the effect was unclear. In order to solve this problem, the present invention is to provide an antioxidant and whitening composition for eating. In addition, the present invention provides a food composition using the antioxidant function of plum and the active oxygen removal function of glutathione yeast.

The present invention provides a means for solving the following problems in order to solve the existing problems.

The antioxidant and whitening composition of the present invention is 0.5% by weight of glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 29.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 0.5% by weight of glutathione. It provides an antioxidant and whitening composition.

In another embodiment, the antioxidant and whitening composition of the present invention is a glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 29% by weight of fructose, 7% of vitamin C, 3% by weight of minerals, and 50% by weight of glutathione. It provides an antioxidant and whitening composition, characterized in that 1% by weight.

In another embodiment, the antioxidant and whitening composition of the present invention is a glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 28.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 50% by weight of glutathione. It provides an antioxidant and whitening composition, characterized in that 1.5% by weight.

chelating 능력을 측정한 결과 Glutathione 효모를 함유한 실험군에서 유의한 저해 효과를 나타내었다. 또한 본 발명의 멜라닌 생합성의 경우에도 X50배를 희석한 Glutathione 효모를 함유한 실험군에서 통계적으로 유의하게 더욱 생합성을 저해하는 효과를 나타내었다. 또한 글루타치온 효모를 이취 없이 섭취하기 위하여 HPMC를 첨가하여 과립화하는 과정에서 항산화 활성과 미백효과는 영향을 받지 않았다.The present invention is a result of the antioxidant experiment by the configuration as described above Fe

As a result of measuring the chelating ability, a significant inhibitory effect was shown in the experimental group containing Glutathione yeast. In addition, in the case of the melanin biosynthesis of the present invention, the experimental group containing the X50-fold diluted Glutathione yeast showed a statistically significant effect of further inhibiting biosynthesis. In addition, the antioxidant activity and whitening effect were not affected in the process of granulating by adding HPMC in order to consume glutathione yeast without off-flavor.

In addition, the present invention provides a composition capable of living a healthy life by providing a food composition using the antioxidant function of plum and the function of removing active oxygen from glutathione yeast.

Figure 1 is Fe chelating ability
Figure 2 is the Tyrosinase inhibition rate
Figure 3 is a Cytotoxicity of extract on B16F0 cells
Figure 4 shows the effect of inhibiting tyrosinase activity in cells
5 is a melanin biosynthesis inhibitory effect
Figure 6 is an antioxidant activity measurement
7 shows The ABTS+ cation radical scavenging activity of containing HPMC products and except HPMC product.
Figure 8 shows Frap The Fe3+-TPTZ-Fe2+-TPTZ reducing ability of containing HPMC products and except HPMC product.
9 shows The Fe2+ chelating of different of containing HPMC products and except HPMC product and except HPMC product.
Figure 10 is Tyrosinase (from mushroom) inhibition rate of containing HPMC products and except HPMC product

Recently, as the level of living and income has improved, a lot of interest is increasing to improve the quality of life. In particular, interest in the development of physiologically active substances for metabolism and diseases in the body, including antioxidants, is increasing as a countermeasure against aging, various inflammatory diseases, and cancer. Biochemical oxidation reactions constantly occur in order to supply energy necessary for the living body, and reactive oxygen species (ROS), which is called harmful oxygen, generated during this process is the most stable form of oxygen, triplet oxygen, which is produced during the oxidation and reduction process. It is unstable with unstable free radicals and hydrogen peroxide (H2O2) such as singlet oxygen superoxide anion, hydroxyl radical, and hydrogen peroxide. (oxidative stress), and among these oxidative stresses, O ₂ and OH, which are important factors of skin light damage, destroy antioxidants present in the skin, initiate lipid peroxidation, oxidation of proteins and DNA, and connective tissue components. It accelerates skin aging such as cleavage of binding chains such as collagen, elastin, and hyaluronic acid, wrinkle formation due to abnormal cross-linking, and promotion of melanin production.

The skin has intrinsc againg, which occurs naturally with age, and photoaging, which is caused by accumulated sunlight exposure. Among them, photoaging is a major cause of skin aging, and it is increasing as the ozone layer is destroyed due to industrialization and environmental pollution, which are rapidly changing in recent years, and the amount of ultraviolet irradiation is seriously released. Ultraviolet (Ultravoiler. UV) is a ray of light with a wavelength of 400 nm or less generated from the sun and causes many changes in the living body. This affects the papillary layer and the reticulum layer of the dermis, and the base layer of the skin is damaged by loss of elasticity, premature aging, expansion and damage of capillaries due to the collapse of elasticity and collagen, and the likelihood of skin cancer is increased. Hair, too, becomes dry when exposed to sunlight, its strength decreases, and its surface becomes rough and brittle. In this way, photochemical decomposition by sunlight stimulates hair or skin proteins and melanin, resulting in destruction of internal cells, cell membrane mixtures, and melanin. Melanin is a complex of black pigment and protein, and it is a pigment that makes up the color of human hair and skin. When the amount is high, the skin color becomes yellowish brown to blackish brown, and the smaller the amount, the lighter color. In the case of melanin, it protects skin cells from the external environment and ultraviolet rays. However, when over-produced due to excessive exposure to ultraviolet rays or skin aging, pigmentation is deposited on the skin to form spots and freckles, and the toxicity of melanin precursors causes skin cancer. It is also possible. Therefore, in order to prevent such pigmentation phenomenon, it is necessary to reduce the production of melanin by inhibiting a part of the melanin production process. The most important step in the biosynthesis of melanin is the initial reaction that occurs through the catalytic action of tyrosinase.In the endoplasmic reticulum called melanosome (melanosome) in the base layer of the epidermis, tyrosinase, tyrosinase related protein It is produced by enzymes such as -1 (TRP-1) and dopachrome tautomerase (DCT).

The aforementioned tyrosinase is an important enzyme that causes the first stage of melanogenesis, and tyrosin (tyrosine) is converted to DOPAquinone (dopaquinone) through 3,4-dihydroxy phenylalanine (DOPA, dopa), and from DOPAquinone. It produces melanin, a dark brown copolymer, through DOPAchrome (Dopachrome) through an automatic oxidation reaction and an enzymatic reaction.

Currently, due to the negative function of excessively produced melanin, studies on the metabolism of melanin biosynthesis are underway in the development of whitening agents. Among them, whitening by inhibiting melanin production by inhibiting the activity of tyrosinase, a major enzyme in melanogenesis. It can induce an effect.

In the existing cosmetics field, as whitening ingredients, hydroquinones, including kojic acid and arbutin, which are known to inhibit tyrosinase enzyme activity, vitamin C, derivatives thereof, and various plant extracts have been used. . However, these substances are unstable, and their use is limited due to decomposition, coloration, off-flavor, unclear effect, safety problems of skin and formulation, as well as mutagenesis and cytotoxicity problems. Therefore, in order to overcome the various disadvantages of existing whitening agents, many studies are being conducted on the development of whitening agents using natural products that are safe for the skin.

Plum tree (Prunus mume) belongs to the Rosaceae family, and its origin is in the southeastern part of China, and it is an endemic fruit tree of the East distributed in the warm regions of Korea, China and Japan. In addition, it has been used for edible or medicinal purposes since ancient times to the extent that it appeared in Nagwanjung’s “Three Kingdoms”. In various oriental medicine books, there are records of treatment of plums such as chronic cough, gastritis, chronic death, hematuria, vomiting, and hemorrhoids. Plums contain a lot of organic acids and minerals such as malic acid, citric acid, oxalic acid, succinic acid, and fumaric acid, and are known to have the effect of promoting appetite and increasing gastric juice secretion to help digestive activity and fatigue recovery. Among them, rutin, one of the important components of plum, helps treat vascular disease, strengthens capillaries, and has anti-inflammatory effects, and is reported to exhibit antioxidant effects, and studies on whitening efficacy using antioxidant effects are also underway. Became.

In the present invention, it was confirmed that a yeast mixture containing plum and glutathione has antioxidant and whitening effects, and it is intended to be utilized.

To this end, an experiment was performed to measure the degree of influence of the antioxidant activity and whitening effect by HPMC added for the antioxidant experiment, melanin biosynthesis experiment, off-flavor removal and granulation.

Experiment method

1) Materials and reagents

DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diamonium salt), Iron (Ⅲ) chloride, Potassium ferricyanide, Trichloroacetic acid, Iron( Ⅱ) chloride, 2,2'-Bipyridyl, Copper (Ⅱ) chloride, Ammonium acetate, Pyrogallol, Quercetin, Folin & Ciocalteu's phenol reagent, Gallic acid, Vanillin, Catechin, TPTZ (2,4,6-tripyridyl-S-triazine ), Ferric Chloride was purchased and used from Sigma-Aldrich (St. Louis, Mo, USA), respectively. Glacial acetic acid was purchased and used from Alfa Aesar (Thermo Fisher Scientific), and sodium acetate trihydrate was purchased and used from JUNSEI.

Dulbecco's Modified Eagle's Medium (DMEM), penicillin-streptomycin, phosphate buffered saline (PBS) and fetal bovine serum (FBS) used for cell culture were purchased from Hyclone, and DMSO and L-Dopa were purchased from Sigma-Aldrich (St. Louis). ,Mo, USA) respectively purchased and used. CCK-8 (Cell Counting Kit-8) was purchased and used from Dojindo (Molecular Technologies, Inc.). For other solvents and reagents used in the study, first-class and special-grade reagents were purchased and used.

chelating ability2) Fe

chelating ability

Fe chelating measurement is measured by modifying the method of Dinis et all. (1994). FeCl solution (30μl, 0.6mM) and Bipyridyl solution (30μl, 5mM) were sequentially added to 120μl of the sample, reacted for 10 minutes, and absorbance was measured at 562nm.

3) Tyrosinase activity inhibition test

To 40 μl of the sample diluted in tertiary distilled water, 80 μl of 67 mM sodium phosphate buffer (pH6.8) was added to buffer. Using the prepared buffer, dissolve 10mM L-DOPA (3,4-dihydroxy-L-phenylalanine) and add 40μl. Finally, 40 μl of 125 units of tyrosinase (from mushroom) was added and reacted at 37° C. for 10 minutes, and then measured at 492 nm.

4) Cell culture

As for the cells used in the experiment, Mouse melanoma melanocyte B16F0 cells were distributed from ATCC and used. B16F0 cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) medium containing 10% heat-inactivated fetal bovine serum (FBS), penicillin and 2-mercaptoethanol at 5% CO _{2 and} 37°C, and used in the experiment.

5) Cell viability measurement

Cell viability was measured using CCK-8 (Cell Counting Kit-8) reagent. B16F0 cells were inoculated into a 96-well plate at a concentration of 1 × 10 ⁵ cells/well, and then samples were administered to each well by concentration, followed by _{incubation in a CO 2} incubator for 72 hours. After 3 hours after adding the CCK-8 solution, the absorbance was measured at 450 nm using a micro plate reader (Bio-tech Co., Ltd.) and compared with the control group.

6) Measurement of the inhibitory effect of intracellular tyrosinase activity

Α-MSH (200 nM) was used as an external stimulator to determine whether the test sample had an effect on the tyrosinase activity in B16F0 cells due to external stimulation, and the inhibition of intracellular tyrosinase activity was measured by modifying the method of Matinez-Esparza. . Cells were aliquoted into a 6-well plate at 1 × 10 ⁶ cells/well and stimulated by treating α-MSH as a positive control. Samples were treated after 1 hour, and after 72 hours incubation, cells were collected and dissolved by adding 1 ml of 0.1 M sodium phosphate buffer (pH 6.5) containing 1% (W/V) Triton X-100. Then, the supernatant obtained by centrifugation was used for tyrosinase activity and protein quantification, and the cell pellet was used for melanin quantification. Dispense 100 μL of the supernatant obtained after sample treatment into a 96-well plate, add 100 μL of 0.1 M sodium phosphate buffer (pH 6.5) to which 0.2 μg/ml 3,4-hydroxyphenylalamine (DOPA) is added, and incubate at 37°C for 2 hours. Then, the absorbance was measured at 490 nm.

7) Melanin quantification

200 μL of a 1N NaOH solution containing 10% DMSO was added to the pellet obtained in the process of measuring the tyrosinase activity, and allowed to stand in a water bath at 90° C. for 30 minutes to completely dissolve melanin, and the absorbance was measured at 475 nm. The amount of melanin was obtained from a standard calibration curve prepared using synthetic melanin, and the amount of melanin in the experimental group was calculated as a percentage of the amount of melanin in the control group. The amount of melanin was expressed as μg/mg protein based on the protein concentration measured in each well.

8) Statistical analysis

All the results were tested in three repetitions, and the measured values were expressed as the mean value ± standard deviation. The statistical significance of the experimental results was set to Student's t- test, and when the p value was less than 0.05, it was judged that there was statistical significance.

( p values ^* <0.05, ^** <0.01, ^*** <0.001 vs. control group, ^# <0.05, ^## <0.01, ^### <0.001 vs. α-MSH group ^$ <0.05, ^$$ <0.01 , ^$$$ <0.001 vs. (-)Glutathione group,)

- Experiment result

chelating ability1) Fe

chelating ability

The chelating ability of divalent iron is an experiment to induce an oxidation reaction of metal ions and to determine how much the samples of the control group and the experimental group inhibit oxidation. As a result, the control group that did not contain Glutathione yeast at a 100-fold dilution concentration (1.5%) More than that, only the experimental group (1.5%) containing Glutathione yeast showed a significant inhibitory effect (Fig. 4).

2) Tyrosinase inhibition rate

Before confirming the intracellular whitening activity, a physicochemical whitening activity experiment was conducted through an enzymatic reaction. In order to prevent the deposition of whitening-related melanin pigment in the body, it is necessary to lower the activity of Tyrosinase. For this purpose, the enzyme activity inhibition rate was measured using Tyrosinase (from mushroom). As a result, in the case of the 100-fold dilution, the experimental group (1.0%, 1.5%) containing Glutathione yeast showed a 16% higher inhibition rate than the control group not containing Glutathione yeast, respectively, 16% at 1.0% and 12% at 1.5% (Fig. 5).

3) Survival rate of B16F0 cells

In order to investigate the effect of the experimental sample on the survival rate of mouse-derived malignant melanoma cells, B16F0 cells, X50 times and X100 times dilution ratios were selected and used in the experiment. As a result of Figure 6, it was confirmed that there was no cytotoxicity (Fig. 6).

4) Inhibitory effect of intracellular tyrosinase activity

Tyrosinase is the most important enzyme involved in the initial reaction, which is the rate-determining step in the melanin synthesis process. In addition, it has tyrosine hydroxylase activity that converts tyrosine to DOPA as an enzyme representing a major step in melanin synthesis, and DOPA oxidase activity that oxidizes DOPA to DOPAquinone. In addition, studies have reported that plum extract inhibits the activity of tyrosinase, and this was to confirm how much more inhibited tyrosinase activity in the experimental sample containing glutathione yeast compared to the control group. In addition, α-MSH (200 nM) was used as an external stimulator to confirm the effect of experimental samples on tyrosinase activity and overproduced melanin in B16FO cells according to external stimulation. As a result, it was confirmed that both samples did not significantly affect the tyrosinase activity of B16f0 cells (Fig. 7).

5) Melanin biosynthesis inhibitory effect

The inhibitory effect of melanin biosynthesis using B16F0 malignant melanoma melanoma cells was measured by modifying the method of Matinez-Esparza. B16F0 malignant melanoma melanoma cells were treated with different dilution ratios and cultured for 72 hours, and then the amount of melanin production was measured. As a result, compared to the control group, the experimental sample containing glutathione yeast showed a statistically significant inhibitory effect on melanin biosynthesis in the X50-fold dilution group, but did not significantly inhibit melanin biosynthesis compared to the positive control group. , It was confirmed that there was no significant difference in the group treated by dilution by X100 times (Fig. 8).

6) Measurement of antioxidant activity

The antioxidant activity of the control group containing 1.5% glutathione yeast, the experimental group to which HPMC was added for granulation, and the experimental group _{dissolved in H 2 O after granulation were compared.} As a result, there was no significant difference in antioxidant activity in the experimental group to which HPMC was added and the experimental group to which H _{2 O was dissolved after granulation compared to the control group.} Therefore, it was confirmed that the antioxidant activity did not decrease due to granulation (Fig. 9-12).

7) Tyrosinase inhibition rate

The inhibition rate of enzyme activity was measured using Tyrosinase (from mushroom) of the control group containing 1.5% glutathione yeast, the experimental group to which HPMC was added for granulation, and the experimental group _{dissolved in H 2 O after granulation.} As a result, in the 100-fold diluted group, there was no significant difference in antioxidant activity in the experimental group to which HPMC was added and the experimental group to which H _{2 O was dissolved after granulation compared to the control group.} Therefore, it was confirmed that the antioxidant activity did not decrease due to granulation.

Antioxidant and whitening composition of the present invention

In the present invention, a mixture of plum and glutathione yeast is granulated and HPMC is added.

The HPMC stands for propylene glycol ether of methylcellulose, and its chemical structure is composed of a cellulose main chain, where the substituent R is H, -CH3 or -CH2CH(CH3)OH.

Cellulose forms crystals due to strong hydrogen bonds of hydroxy groups and is not well soluble in water, but HPMC has high solubility in water and is well soluble at low temperatures, forming a viscous colloidal solution. It is hardly soluble in organic solvents such as chloroform, ethanol, and ether, but it is soluble in a mixture of ethanol and dichloromethane, methanol and dichloromethane, and water and alcohol. In general, it is a tasteless, odorless white powder that is not irritating or toxic, and is harmless to the human body.

The physicochemical properties of HPMC are related to i) the ratio of -OCH3 groups, ii) the ratio of -OCH2CH(CH3)OH groups, and iii) the molecular weight. The United States Pharmacopoeia (USP) defines the types of HPMC as HPMC1828, HPMC2208(K), HPMC2906(F), and HPMC2910(E) according to the ratio of -OCH3, -OCH2CH(CH3)OH as shown in Table 1. The first two numbers are the ratio of the -OCH3 group, and the second two numbers are the ratio of the -OCH2CH(CH3)OH group. All grades of HPMC are widely used in various formulations, especially those used for drug release control are mainly grades 2208 and 2910, which have relatively high viscosity.

The US Pharmacopoeia (USP) suggests that HPMC's viscosity measurement method is made into a 2% solution at 20°C. At this time, the viscosity of HPMC varies greatly depending on the measurement temperature, because HPMC has a property of changing to a gel at a specific temperature. The sol-gel-zolo phase transition phenomenon according to temperature is because a polymer is formed by the interaction between hydrophobic functional groups in the molecule. Another classification method is classified into P: premium, LV: low viscosity, CR: controlled release, G: granular, S: surface treated, and FG: food grade, depending on the grade of HPMC.

In the present invention, HPMC was used as a binder to coat, combine, and granulate plum and glutathione yeast. This configuration prevented the user from feeling the off-flavor generated from glutathione yeast when ingested.

In the present invention, plum concentrate, crystal fructose, xylitol, minerals, vitamins, glutathione yeast (containing 50% glutathione), and glucose were mixed and used in the following samples 1 to 3 in order to improve the convenience and function of the user's intake.

The antioxidant and whitening composition of the present invention is 0.5% by weight of glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 29.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 0.5% by weight of glutathione. It provides an antioxidant and whitening composition.

In another embodiment, the antioxidant and whitening composition of the present invention is a glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 29% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 50% by weight of glutathione. It provides an antioxidant and whitening composition, characterized in that 1% by weight.

In another embodiment, the antioxidant and whitening composition of the present invention is a glutathione yeast containing 5% by weight of plum, 50% by weight of xylitol, 28.5% by weight of fructose, 7% of vitamin C, 3% by weight of minerals, and 50% by weight of glutathione. It provides an antioxidant and whitening composition, characterized in that 1.5% by weight.

The composition blended in the above blending ratio was granulated by the following method.

A mixed composition preparation step (S1) of preparing a liquid material by mixing xylitol, crystal fructose, vitamin C, and mineral glutathione yeast powder (containing 50% glutathione) based on plum concentrate (10 Bix); And

A granulation step (S2) of granulating using a sugar seed as a nucleus for granulation by setting the process temperature of the fluid bed granulator to 50°C;

Antioxidant comprising plum and glutathione yeast, comprising: an HMPC coating process (S3) in which the granules of the composition granulated in the granulation process are coated with HMPC to absorb moisture and prevent agglomeration of the granules. Provides a whitening composition.

It provides an antioxidant and whitening composition comprising plum and glutathione yeast, characterized in that the sugar seed has a diameter of 0.3mm.

The process temperature is characterized in that less than 60 ℃. This is because when the temperature reaches 60° C., all of the mixed composition is dissolved and granulation is impossible.

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Claims (3)

A mixed composition prepared by mixing 5% by weight of plum, 50% by weight of xylitol, 29.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 0.5% by weight of glutathione yeast containing 50% by weight of glutathione Step (S1); And
A granulation step (S2) of granulating using a sugar seed as a nucleus for granulation by setting the process temperature of the fluid bed granulator to 50°C;
Antioxidant comprising plum and glutathione yeast, comprising: an HMPC coating process (S3) in which the granules of the composition granulated in the granulation process are coated with HMPC to absorb moisture and prevent agglomeration of the granules. Whitening composition. A mixed composition prepared by mixing 5% by weight of plum, 50% by weight of xylitol, 29% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 1% by weight of glutathione yeast containing 50% by weight of glutathione Step (S1); And
A granulation step (S2) of granulating using a sugar seed as a nucleus for granulation by setting the process temperature of the fluid bed granulator to 50°C;
Antioxidant comprising plum and glutathione yeast, comprising: an HMPC coating process (S3) in which the granules of the composition granulated in the granulation process are coated with HMPC to absorb moisture and prevent agglomeration of the granules. Whitening composition. A mixed composition prepared by mixing 5% by weight of plum, 50% by weight of xylitol, 28.5% by weight of crystalline fructose, 7% of vitamin C, 3% by weight of minerals, and 1.5% by weight of glutathione yeast containing 50% by weight of glutathione Step (S1); And
A granulation step (S2) of granulating using a sugar seed as a nucleus for granulation by setting the process temperature of the fluid bed granulator to 50°C;
Antioxidant comprising plum and glutathione yeast, comprising: an HMPC coating process (S3) in which the granules of the composition granulated in the granulation process are coated with HMPC to absorb moisture and prevent agglomeration of the granules. Whitening composition.

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