KR102235665B1 - Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof - Google Patents

Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof Download PDF

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KR102235665B1
KR102235665B1 KR1020190148021A KR20190148021A KR102235665B1 KR 102235665 B1 KR102235665 B1 KR 102235665B1 KR 1020190148021 A KR1020190148021 A KR 1020190148021A KR 20190148021 A KR20190148021 A KR 20190148021A KR 102235665 B1 KR102235665 B1 KR 102235665B1
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primer
dna
artificial sequence
melon
sequence
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권석윤
신아영
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한국생명공학연구원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

The present invention relates to a set of single nucleotide polymorphism marker set for backcross breeding of oriental melons and uses thereof. The 320 SNP markers of the present invention are distributed throughout 12 chromosomes of oriental melons, and the genetic background or variety of oriental melons can be quickly and accurately discriminated, thereby being expected to be used as a useful molecular marker in the cultivation of novel oriental melon varieties.

Description

참외의 마커이용여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도{Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof}Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof}

본 발명은 참외의 마커이용여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도에 관한 것이다.The present invention relates to a set of monobasic polymorphism markers for cross-breeding breeding using a marker of melon and its use.

참외(Cucumis melo var. makuwa)는 박과에 속하는 덩굴식물로 분류학적으로 멜론(Cucumis melo)의 한 변종이다. 박과 작물의 국내 종자 시장은 320억 정도로 전체 채소 종자시장의 약 20%를 차지하고 있으며, 그 중 참외는 박과 작물 중 수박에 이어 2번째로 종자시장 규모가 크고 매년 시장 규모와 재배 면적이 다소 증가하는 추세에 있다. 참외는 국내에서는 경제적, 상업적으로 중요한 작물이며, 수출 품종 육성의 중요성도 눈에 띄게 대두되고 있다. 참외는 세대가 짧고 타 작물에 비해 고정이 잘되어 계통 개발에 걸리는 시간이 매우 짧기 때문에, 개발된 품종 및 계통의 보호를 위한 분자표지 개발이 필수적이다. 참외의 유전체 해독이 이루어지고 있고 NGS(next generation sequencing)을 이용한 대용량 염기서열 생산이 가속화되면서 육종 산업 내 유전체 기반의 분자 육종이 가지는 중요도는 더욱 커지고 있다.Melon ( Cucumis melo var. makuwa ) is a vine plant belonging to the cucurbitaceae family, taxonomically a variety of melon (Cucumis melo ). The domestic seed market of gourd crops is about 32 billion won, accounting for about 20% of the total vegetable seed market, of which melon is the second largest seed market after watermelon among gourds, and the market size and cultivation area are somewhat larger every year. It is on an increasing trend. Melons are economically and commercially important crops in Korea, and the importance of cultivating export varieties is remarkably emerging. Since melon has a short generation and is well fixed compared to other crops, the time it takes to develop a line is very short, so it is essential to develop a molecular marker for the protection of the developed variety and line. The importance of genome-based molecular breeding in the breeding industry is increasing as the genome of melon is being decoded and the production of large-capacity sequences using NGS (next generation sequencing) is accelerated.

분자육종은 원하는 형질의 유무를 형질에서 유래한 표현형의 관찰없이 DNA 염기서열의 차이를 보이는 분자마커를 이용하여 판별하는 기법으로, 전통적 선발 육종 방법에 비해 육종 연한을 단축시키고 우량 개체의 선발 효율을 극대화시킬 수 있는 장점을 지니고 있다. 더욱이 분자마커를 여교잡 육종에 적용하는 마커이용여교잡(marker assisted backcross, MAB)은 분자마커를 이용하여 유묘기에 여교잡 자손의 염색체 조성을 확인함으로써 신속하고 정확하게 회복친의 염색체 이입개체를 선발할 수 있어 육종 연한과 비용, 노력 등을 효율적으로 감소시킬 수 있다. 최근 MAB에 대표적으로 이용되는 분자마커는 유전체 내 가장 높은 빈도로 존재하는 단일염기다형성(single nucleotide polymorphism, SNP) 마커이며, 다양한 식물체의 품종 구분 또는 유용 형질 유전자 선발을 위한 연구에 폭넓게 활용되고 있다. 이러한 MAB 육종 방법은 분리세대에서 유전자형을 정확히 파악 선발할 수 있는 정밀육종을 가능하게 하고 유용한 대립 유전자를 골라 집적시킬 수 있기 때문에 맞춤형 작물 시대를 열고 있다.Molecular breeding is a technique for discriminating the presence or absence of a desired trait using molecular markers that show differences in DNA sequences without observing the phenotype derived from the trait. Compared to the traditional selection breeding method, the breeding period is shortened and the selection efficiency of superior individuals is improved. It has an advantage that can be maximized. Furthermore, marker assisted backcross (MAB), which applies molecular markers to female hybrid breeding, uses molecular markers to check the chromosome composition of female hybrid offspring at the seedling period, so that it can quickly and accurately select chromosomal transgenics of the recovered parent. It can effectively reduce the age, cost, and effort of breeding. Recently, the most commonly used molecular markers for MAB are single nucleotide polymorphism (SNP) markers that exist at the highest frequency in the genome, and are widely used in research for differentiating varieties of various plants or selecting useful trait genes. This MAB breeding method is opening the era of customized crops because it enables precise breeding that can accurately identify and select genotypes in isolated generations, and can select and accumulate useful alleles.

한편, 한국등록특허 제1923647호에 분자마커이용여교잡을 위한 '쥬빌리 타입 또는 크림슨 타입 수박 품종 판별용 SNP 마커'가 개시되어 있고, 한국등록특허 제1774275호에 '고추의 여교배 육종을 위한 단일염기다형성 마커 및 이의 용도'가 개시되어 있으나, 본 발명의 참외의 마커이용여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도에 대해서는 기재된 바가 없다.On the other hand, Korean Patent No. 1923647 discloses a'SNP marker for discriminating Jubilee type or Crimson type watermelon varieties' for hybridization using molecular markers. Although nucleotide polymorphism markers and their uses' are disclosed, there is no description of a single nucleotide polymorphism marker set and a use thereof for crossbreeding breeding using the melon marker of the present invention.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 재래종 참외의 고품질 유전체 정보를 구축하고 다양한 참외 계통의 유전자원을 비교·분석하여 참외 12개의 염색체에서 총 320개의 단일염기다형성(single nucleotide polymorphism, SNP) 마커를 선발한 후 상기 SNP 마커를 증폭시키기 위한KASP(kompetitive allele specific PCR)용 프라이머 세트를 제작하였다. 상기 제작된 프라이머 세트를 이용하여 참외 육성계통 30개의 유전적 배경을 효과적으로 구별할 수 있음을 확인함으로써, 본 발명을 완성하였다.The present invention was derived from the above requirements, and the present inventors constructed high-quality genome information of native melon species, compared and analyzed the genetic resources of various melon lineages, and analyzed a total of 320 single nucleotide polymorphisms in 12 melon chromosomes. After selecting a polymorphism, SNP) marker, a primer set for kompetitive allele specific PCR (KASP) was prepared to amplify the SNP marker. By confirming that the genetic background of 30 melon breeding lines can be effectively distinguished using the prepared primer set, the present invention was completed.

상기 과제를 해결하기 위해, 본 발명은 서열번호 1 내지 960으로 표시된 올리고뉴클레오티드에서 서열번호 1부터 시작하여 인접한 3개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 320개의 올리고뉴클레오티드 프라이머 세트로 이루어진 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 마커이용여교잡(marker assisted backcross) 육종을 위한 참외(Cucumis melo var. makuwa)의 유전적 배경 구별용 KASP(kompetiive allele specific PCR)용 프라이머 세트를 제공한다.In order to solve the above problem, the present invention is a group consisting of 320 oligonucleotide primer sets, characterized in that three adjacent oligonucleotides starting from SEQ ID NO: 1 in the oligonucleotides represented by SEQ ID NOs: 1 to 960 are one primer set. It provides a primer set for KASP (kompetiive allele specific PCR) for distinguishing the genetic background of melon (Cucumis melo var. makuwa ) for marker assisted backcross breeding, including one or more primer sets selected from .

또한, 본 발명은 상기 프라이머 세트; 및 증폭 반응을 수행하기 위한 시약을 포함하는, 마커이용여교잡 육종을 위한 참외의 유전적 배경 구별용 키트를 제공한다.In addition, the present invention is the primer set; And it provides a kit for distinguishing the genetic background of melon for hybrid breeding using a marker, comprising a reagent for performing an amplification reaction.

또한, 본 발명은 참외 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 상기 프라이머 세트를 이용하여 KASP를 수행하는 단계; 및 상기 KASP의 증폭산물을 분석하는 단계;를 포함하는, 마커이용여교잡 육종을 위한 참외의 유전적 배경을 구별하는 방법을 제공한다.In addition, the present invention comprises the steps of separating genomic DNA from a melon sample; Performing KASP using the isolated genomic DNA as a template and using the primer set; And analyzing the amplification product of the KASP; It provides a method for distinguishing the genetic background of melon for hybrid breeding using a marker comprising a.

본 발명의 KASP 프라이머 세트는 여교배 후 분리집단의 개체들이 가지고 있는 반복친과 회복친의 유전적 배경을 확인하기 위해 소요되는 시간과 노력을 획기적으로 단축할 수 있어 참외 품종 육성에 유용하게 활용될 수 있을 뿐만 아니라, SNP 데이터베이스를 축적함으로써 참외의 양적·질적 형질 연구에 바탕을 마련할 수 있을 것으로 기대된다.The KASP primer set of the present invention can dramatically shorten the time and effort required to check the genetic background of the recurrent parent and the recovered parent that the individuals of the isolated group have after female cross-breeding, so that it can be usefully utilized for cultivation of melon varieties. Not only that, but by accumulating the SNP database, it is expected that it will be able to lay the groundwork for the study of quantitative and qualitative traits of melon.

도 1은 참외의 12개 염색체상에 존재하는 SNP를 나타낸 것이다.
도 2 내지 7은 본 발명의 KASP 프라이머 세트 320개를 이용하여 30개의 참외 육성계통에서 유전형 분석을 수행한 결과이다. A, B: 동형접합체, H: 이형접합체.1 shows SNPs present on 12 chromosomes of melon.
2 to 7 are results of genotyping in 30 melon breeding lines using 320 KASP primer sets of the present invention. A, B: homozygous, H: heterozygous.

본 발명의 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 960으로 표시된 올리고뉴클레오티드에서 서열번호 1부터 시작하여 인접한 3개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 320개의 올리고뉴클레오티드 프라이머 세트로 이루어진 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 마커이용여교잡(marker assisted backcross) 육종을 위한 참외(Cucumis melo var. makuwa)의 유전적 배경 구별용 KASP(kompetiive allele specific PCR)용 프라이머 세트를 제공한다.In order to achieve the object of the present invention, the present invention is a set of 320 oligonucleotide primers, characterized in that three adjacent oligonucleotides starting from SEQ ID NO: 1 in the oligonucleotides represented by SEQ ID NOs: 1 to 960 are one primer set. A primer set for KASP (kompetiive allele specific PCR) for distinguishing the genetic background of melon (Cucumis melo var. makuwa ) for marker assisted backcross breeding, comprising one or more primer sets selected from the group consisting of: to provide.

본 발명의 용어 "KASP(kompetitive allele specific PCR)"는 PCR(polymerase chain reaction) 기반의 분석 방법 중 하나로, 상동의(homogenous) 그리고 형광(fluorescence) 기반의 유전형 분석 기술이다. KASP는 대립형질(allele)-특이적 올리고 연장(extension) 및 신호생성을 위한 형광공명에너지전이(fluorescence resonance energy transfer)를 기반으로 하는 기술이다.The term "kompetitive allele specific PCR (KASP)" of the present invention is one of a polymerase chain reaction (PCR)-based analysis method, and is a homogenous and fluorescence-based genotyping analysis technique. KASP is a technology based on fluorescence resonance energy transfer for allele-specific oligo extension and signal generation.

본 발명의 KASP 프라이머 세트는 참외 유전자좌에서 특이적으로 차별화되는 단일염기다형성(single nucleotide polymorphism, SNP) 마커의 염기 타입을 검출하는 프라이머 세트이다. 본 발명의 KASP 프라이머 세트는 30개의 다양한 참외 육성계통의 게놈 서열을 이용하여 제작되었으며, 소수의 참외 품종에 국한되지 않고 상업용 참외 품종에 대한 유전적 배경 분석 및 품종 구별이 가능하다. The KASP primer set of the present invention is a primer set that detects the base type of a single nucleotide polymorphism (SNP) marker that is specifically differentiated at a melon locus. The KASP primer set of the present invention was produced using the genome sequence of 30 various melon breeding lines, and it is not limited to a few melon varieties, and genetic background analysis and cultivar identification for commercial melon varieties are possible.

본 발명의 상기 프라이머 세트는 연속되는 서열번호 3개의 올리고뉴클레오티드가 하나의 프라이머 세트를 이루며, 2개의 정방향 프라이머와 1개의 역방향 프라이머로 구성되어 있다. 본 발명의 프라이머 세트에 있어서 인접한 3개의 올리고뉴클레오티드는 순서대로 FAM이 결합된 정방향 프라이머, HEX가 결합된 정방향 프라이머 및 역방향 프라이머로 구성되어 있으며, 구체적으로 정방향 프라이머의 5' 말단에는 FAM 또는 HEX 형광물질이 부착되어 있고 3' 말단에는 SNP를 나타내는 염기가 결합되어 있으며, 공통의 역방향 프라이머 세트 1개로 구성되어 있다.In the primer set of the present invention, three consecutive oligonucleotides of SEQ ID NOs form one primer set, and are composed of two forward primers and one reverse primer. In the primer set of the present invention, the three adjacent oligonucleotides are sequentially composed of a FAM-coupled forward primer, a HEX-coupled forward primer, and a reverse primer, and specifically, a FAM or HEX fluorescent substance at the 5'end of the forward primer Is attached, and a base representing SNP is bonded to the 3'end, and is constituted by one common set of reverse primers.

본 발명의 프라이머 세트는 바람직하게는 상기 320개의 프라이머 세트로 이루어진 군으로부터 선택되는 1개 이상의 프라이머 세트를 포함할 수 있으나, 상기 320개의 프라이머 세트를 조합하여 이용하거나 동시에 이용하면 참외의 유전적 배경을 더욱 효율적으로 구별할 수 있다.The primer set of the present invention may preferably include one or more primer sets selected from the group consisting of the 320 primer sets, but if the 320 primer sets are used in combination or at the same time, the genetic background of melon They can be distinguished more efficiently.

본 발명의 상기 프라이머는 각 프라이머의 서열 길이에 따라 15개 이상, 16개 이상, 17개 이상, 18개 이상, 19개 이상, 20개 이상, 21개 이상, 22개 이상, 23개 이상, 24개 이상, 25개 이상, 26개 이상의 연속 뉴클레오티드의 절편으로 이루어진 올리고뉴클레오티드를 포함할 수 있다. 예를 들면, 서열번호 1의 프라이머(43개 올리고뉴클레오티드)는 서열번호 1의 서열 내의 38개 이상, 39개 이상, 40개 이상, 41개 이상, 42개 이상의 뉴클레오티드의 절편으로 이루어진 올리고뉴클레오티드를 포함할 수 있다. The primers of the present invention are 15 or more, 16 or more, 17 or more, 18 or more, 19 or more, 20 or more, 21 or more, 22 or more, 23 or more, 24 or more, depending on the sequence length of each primer. It may include an oligonucleotide consisting of segments of at least two, at least 25, or at least 26 contiguous nucleotides. For example, the primer of SEQ ID NO: 1 (43 oligonucleotides) includes an oligonucleotide consisting of fragments of 38 or more, 39 or more, 40 or more, 41 or more, 42 or more nucleotides in the sequence of SEQ ID NO: 1. can do.

본 발명에 있어서, "프라이머"는 카피하려는 핵산 가닥에 상보적인 단일 가닥 올리고뉴클레오티드 서열을 말하며, 프라이머 연장 산물의 합성을 위한 개시점으로서 작용할 수 있다. 상기 프라이머의 길이 및 서열은 연장 산물의 합성을 시작하도록 허용해야 한다. 프라이머의 구체적인 길이 및 서열은 요구되는 DNA 또는 RNA 표적의 복합도(complexity)뿐만 아니라 온도 및 이온 강도와 같은 프라이머 이용 조건에 의존할 것이다.In the present invention, "primer" refers to a single-stranded oligonucleotide sequence that is complementary to a nucleic acid strand to be copied, and may serve as an initiating point for the synthesis of a primer extension product. The length and sequence of the primers should allow the synthesis of the extension product to begin. The specific length and sequence of the primers will depend on the conditions of use of the primer, such as temperature and ionic strength, as well as the complexity of the required DNA or RNA target.

본 발명에 있어서, 프라이머로서 이용된 올리고뉴클레오티드는 또한 뉴클레오티드 유사체(analogue), 예를들면, 포스포로티오에이트(phosphorothioate), 알킬포스포로티오에이트 또는 펩티드 핵산 (peptide nucleic acid)을 포함할 수 있거나 또는 삽입 물질(intercalating agent)을 포함할 수 있다. 또한, 프라이머는 DNA 합성의 개시점으로 작용하는 프라이머의 기본 성질을 변화시키지 않는 추가의 특징을 혼입할 수 있다. 본 발명의 프라이머 핵산 서열은 필요한 경우, 분광학적, 광화학적, 생화학적, 면역화학적 또는 화학적 수단에 의해 직접적으로 또는 간접적으로 검출 가능한 표지를 포함할 수 있다. 표지의 예로는, 효소(예를 들어, HRP (horse radish peroxidase), 알칼리 포스파타아제), 방사성 동위원소(예를 들어, 32P), 형광성 분자, 화학그룹(예를 들어, 비오틴) 등이 있다.In the present invention, the oligonucleotide used as a primer may also comprise a nucleotide analogue, e.g., phosphorothioate, alkylphosphorothioate or peptide nucleic acid, or It may contain an intercalating agent. In addition, the primers can incorporate additional features that do not change the basic properties of the primers serving as an initiation point for DNA synthesis. If necessary, the primer nucleic acid sequence of the present invention may include a label detectable directly or indirectly by spectroscopic, photochemical, biochemical, immunochemical or chemical means. Examples of labels include enzymes (e.g., horse radish peroxidase (HRP), alkaline phosphatase), radioactive isotopes (e.g., 32 P), fluorescent molecules, chemical groups (e.g., biotin), etc. have.

프라이머의 적합한 길이는 사용하고자하는 프라이머의 특성에 의해 결정된다. 프라이머는 주형의 서열과 정확하게 상보적일 필요는 없지만 주형과 혼성복합체(hybrid-complex)를 형성할 수 있을 정도로 상보적이어야만 한다.The suitable length of a primer is determined by the properties of the primer to be used. The primer does not have to be exactly complementary to the sequence of the template, but must be complementary enough to form a hybrid-complex with the template.

본 발명은 또한, 본 발명의 프라이머 세트; 및 증폭 반응을 수행하기 위한 시약을 포함하는, 마커이용여교잡 육종을 위한 참외의 유전적 배경 구별용 키트를 제공한다. The present invention also includes the primer set of the present invention; And it provides a kit for distinguishing the genetic background of melon for hybrid breeding using a marker, comprising a reagent for performing an amplification reaction.

본 발명의 키트에서, 상기 증폭 반응을 수행하기 위한 시약은 DNA 폴리머라제, dNTPs, 및 버퍼를 포함할 수 있으나, 이에 제한되는 것은 아니다. 상기 dNTPs는 dATP, dCTP, dGTP, dTTP를 포함하며, DNA 폴리머라제는 내열성 DNA 중합효소로서 Taq DNA 폴리머라제, Tth DNA 폴리머라제 등 시판되는 폴리머라제를 이용할 수 있다. 또한, 본 발명의 키트는 최적의 반응 수행 조건을 기재한 사용자 설명서를 추가로 포함할 수 있다. 안내서는 키트 사용법, 예를 들면, 역전사 완충액 및 PCR 완충액 제조 방법, 제시되는 반응 조건 등을 설명하는 인쇄물이다. 안내서는 팜플렛 또는 전단지 형태의 안내 책자, 키트에 부착된 라벨, 및 키트를 포함하는 패키지의 표면상에 설명을 포함한다. 또한, 안내서는 인터넷과 같이 전기 매체를 통해 공개되거나 제공되는 정보를 포함한다.In the kit of the present invention, reagents for performing the amplification reaction may include DNA polymerase, dNTPs, and buffers, but are not limited thereto. The dNTPs include dATP, dCTP, dGTP, and dTTP, and the DNA polymerase may be a commercially available polymerase such as Taq DNA polymerase or Tth DNA polymerase as a heat-resistant DNA polymerase. In addition, the kit of the present invention may further include a user's manual describing the optimum reaction performance conditions. The guide is a handout explaining how to use the kit, e.g., how to prepare reverse transcription and PCR buffers, and the reaction conditions presented. The guide includes a brochure in the form of a pamphlet or flyer, a label affixed to the kit, and a description on the surface of the package containing the kit. In addition, the guide includes information disclosed or provided through electronic media such as the Internet.

본 발명은 또한,The present invention also,

참외 시료에서 게놈 DNA를 분리하는 단계; Separating genomic DNA from the melon sample;

상기 분리된 게놈 DNA를 주형으로 하고, 본 발명의 프라이머 세트를 이용하여 KASP를 수행하는 단계; 및 Performing KASP using the isolated genomic DNA as a template and using the primer set of the present invention; And

상기 KASP의 증폭산물을 분석하는 단계;를 포함하는, 마커이용여교잡 육종을 위한 참외의 유전적 배경을 구별하는 방법을 제공한다.Analyzing the amplification product of the KASP; It provides a method for discriminating the genetic background of melon for hybrid breeding using markers.

본 발명의 참외의 유전적 배경을 구별하는 방법에 있어서, KASP 프라이머 세트는 전술한 것과 같다.In the method for discriminating the genetic background of melon of the present invention, the KASP primer set is the same as described above.

본 발명의 방법은 참외 시료에서 게놈 DNA를 분리하는 단계를 포함한다. 상기 게놈 DNA를 분리하는 방법은 당업계에 공지된 방법을 이용할 수 있으며, 예를 들면, CTAB 방법을 이용할 수도 있고, Wizard prep 키트(Promega 사)를 이용할 수도 있다. 상기 분리된 게놈 DNA를 주형으로 하고, 본 발명의 일 실시예에 따른 프라이머 세트를 프라이머로 이용하여 증폭 반응을 수행하여 표적 서열을 증폭할 수 있다. 표적 핵산을 증폭하는 방법은 중합효소연쇄반응(polymerase chain reaction; PCR), 리가아제 연쇄반응(ligase chain reaction), 핵산 서열 기재 증폭(nucleic acid sequence-based amplification), 전사 기재 증폭시스템(transcription-based amplification system), 가닥 치환 증폭(strand displacement amplification) 또는 Qβ 복제효소(replicase)를 통한 증폭 또는 당업계에 알려진 핵산 분자를 증폭하기 위한 임의의 기타 적당한 방법이 있다. 이 중에서, PCR이란 중합효소를 이용하여 표적 핵산에 특이적으로 결합하는 프라이머 쌍으로부터 표적 핵산을 증폭하는 방법이다. 이러한 PCR 방법은 당업계에 잘 알려져있으며, 상업적으로 이용가능한 키트를 이용할 수도 있다.The method of the present invention includes the step of isolating genomic DNA from a melon sample. As a method for separating the genomic DNA, a method known in the art may be used, for example, a CTAB method or a Wizard prep kit (Promega). Using the isolated genomic DNA as a template, a target sequence may be amplified by performing an amplification reaction using the primer set according to an embodiment of the present invention as a primer. Methods of amplifying target nucleic acids include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, and transcription-based amplification system. amplification system), strand displacement amplification or amplification via Qβ replicase or any other suitable method for amplifying nucleic acid molecules known in the art. Among them, PCR is a method of amplifying a target nucleic acid from a pair of primers that specifically bind to a target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used.

본 발명의 방법에 있어서, 상기 증폭된 서열은 검출가능한 표지 물질로 표지될 수 있다. 일 구현예에서, 상기 표지 물질은 형광, 인광 또는 방사성을 발하는 물질일 수 있으나, 이에 제한되지 않는다. 바람직하게는, 상기 표지 물질은 FAM, HEX, VIC, JOE, ROX, TAMRA, Cy3 또는 Cy5 등일 수 있다. 표적 서열의 증폭시 프라이머의 5'-말단에 상기 표지 물질을 표지하여 PCR을 수행하면 표적 서열이 검출가능한 형광 표지 물질로 표지될 수 있다. 표적 서열을 증폭하기 위해 이용된 올리고뉴클레오티드 프라이머 조합은 상기에 기재된 바와 같다.In the method of the present invention, the amplified sequence may be labeled with a detectable labeling material. In one embodiment, the labeling material may be a material that emits fluorescence, phosphorescence, or radioactivity, but is not limited thereto. Preferably, the labeling material may be FAM, HEX, VIC, JOE, ROX, TAMRA, Cy3 or Cy5. When the amplification of the target sequence is performed by labeling the labeling material at the 5'-end of the primer and performing PCR, the target sequence may be labeled with a detectable fluorescent labeling material. The oligonucleotide primer combinations used to amplify the target sequence are as described above.

또한, 본 발명의 일 구현예에 따른 방법에 있어서, 상기 KASP의 증폭산물을 분석하는 단계는 유전형 분석을 통해 수행될 수 있다. 구체적으로는, 정방향 프라이머의 5' 말단에 부착되어 있는 형광물질 FAM 또는 HEX와, 3' 말단에 결합된 SNP를 통해 유전형을 구별할 수 있다.In addition, in the method according to an embodiment of the present invention, the step of analyzing the amplification product of KASP may be performed through genotyping. Specifically, genotypes can be distinguished through the fluorescent substance FAM or HEX attached to the 5'end of the forward primer and the SNP attached to the 3'end.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

재료 및 방법Materials and methods

1. SNP 마커 정보1. SNP marker information

참조 유전체를 작성하기 위해 곶감참외를 대상으로 차세대 염기서열 분석(Next Generation Sequencing, NGS) 및 Hi-C 방법으로 유전체 정보를 얻었고 다양한 생물정보 분석 방법을 통해 12개의 염색체로 구성된 참외 게놈 정보를 구축하였다(한국생명공학연구원 식물시스템공학연구센터 보유). 야생종 참외를 포함한 36개의 다양한 재래종 참외 및 참외 계통의 유전체 분석을 위한 NGS 리드(read)를 생산하였고, 생산된 리드는 퀄리티가 낮은 서열을 제거한 후 BWA(Burrows-Wheeler Alignment tool)를 이용하여 곶감참외 참조서열에 맵핑하였다. 맵핑된 리드들은 SNP 콜링(calling)의 정확도를 높이기 위해 게놈 분석 툴인 GATK(http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit)를 이용하여 이중 마스킹(duplicate masking), 국소적 재정렬(local realignment) 과정을 수행한 후 UnifiedGenotyper를 이용해 SNP(single nucleotide polymorphism)를 예측하고 필터링 과정을 거쳐 320개의 SNP를 선별하여 염색체별로 정리하였다(표 1 및 도 1).To create a reference genome, genome information was obtained from dried persimmon melon by Next Generation Sequencing (NGS) and Hi-C method, and information on the melon genome consisting of 12 chromosomes was constructed through various bioinformation analysis methods. (Plant System Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology). We produced NGS reads for genome analysis of 36 different native melon and melon strains including wild melon, and dried persimmon melon using BWA (Burrows-Wheeler Alignment tool) after removing low-quality sequences. Mapped to the reference sequence. Mapped reads are double masked and localized using GATK (http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit), a genome analysis tool, to increase the accuracy of SNP calling. After performing a local realignment process, single nucleotide polymorphism (SNP) was predicted using UnifiedGenotyper, and 320 SNPs were selected through a filtering process and organized by chromosome (Table 1 and FIG. 1).

염색체별로 선별된 SNP 마커SNP markers selected for each chromosome 염색체chromosome 길이 (Kb)Length (Kb) SNP 수SNP number chr01chr01 30,382.07130,382.071 2828 chr02chr02 22,746.49422,746.494 2525 chr03chr03 21,121.64521,121.645 2424 chr04chr04 29,198.73029,198.730 3131 chr05chr05 27,871.97027,871.970 3030 chr06chr06 34,894.54434,894.544 3030 chr07chr07 23,387.21223,387.212 3131 chr08chr08 32,358.24532,358.245 3535 chr09chr09 19,018.73219,018.732 1919 chr10chr10 19,826.10319,826.103 2323 chr11chr11 26,513.79626,513.796 2828 chr12chr12 20,136.14320,136.143 1616 합계Sum 307,455.685307,455.685 320320

2. SNP 기반 KASP 프라이머 제작2. SNP-based KASP primer production

상기 선별된 320개의 SNP를 기반으로 제작된 KASP(Kompetitive allele specific PCR) 프라이머 세트는 2개의 정방향 프라이머와 1개의 역방향 프라이머로 구성되어 있다. 정방향 프라이머는 5' 말단에 FAM 또는 HEX 형광물질이 부착되어 있고 3' 말단에는 SNP와 같은 염기서열의 차이가 구분되도록 디자인하였다. 그리고 역방향 프라이머는 상기 2개의 정방향 프라이머와 작동하여 PCR 반응이 진행될 수 있도록 디자인하였다. PCR을 수행하면 2개의 정방향 프라이머의 5' 말단에 각기 다른 형광물질이 붙은 채로 증폭되므로, 형광물질의 파장 차이에 따라 유전형을 분석하였다. 유전형이 동형접합체(homozygous)라면 두 개의 형광(FAM 또는 HEX) 중 한 개의 형광만 검출된 것으로, 참조 유전체에 존재하는 SNP는 FAM이 부착된 정방향 프라이머에 의해 검출될 수 있도록 디자인하였다(표 2 내지 표 18). The KASP (Kompetitive allele specific PCR) primer set constructed based on the selected 320 SNPs consists of two forward primers and one reverse primer. The forward primer was designed so that the FAM or HEX fluorescent material was attached to the 5'end and the difference in base sequence such as SNP was distinguished at the 3'end. And the reverse primer was designed so that the PCR reaction could proceed by working with the two forward primers. When PCR was performed, amplification was performed with different fluorescent substances attached to the 5'ends of the two forward primers, so the genotype was analyzed according to the wavelength difference of the fluorescent substances. If the genotype is homozygous, only one of the two fluorescence (FAM or HEX) was detected, and the SNP present in the reference genome was designed to be detected by the FAM-attached forward primer (Table 2 to Table 18).

Figure 112019118326229-pat00001
Figure 112019118326229-pat00001

Figure 112019118326229-pat00002
Figure 112019118326229-pat00002

Figure 112019118326229-pat00003
Figure 112019118326229-pat00003

Figure 112019118326229-pat00004
Figure 112019118326229-pat00004

Figure 112019118326229-pat00005
Figure 112019118326229-pat00005

Figure 112019118326229-pat00006
Figure 112019118326229-pat00006

Figure 112019118326229-pat00007
Figure 112019118326229-pat00007

Figure 112019118326229-pat00008
Figure 112019118326229-pat00008

Figure 112019118326229-pat00009
Figure 112019118326229-pat00009

Figure 112019118326229-pat00010
Figure 112019118326229-pat00010

Figure 112019118326229-pat00011
Figure 112019118326229-pat00011

Figure 112019118326229-pat00012
Figure 112019118326229-pat00012

Figure 112019118326229-pat00013
Figure 112019118326229-pat00013

Figure 112019118326229-pat00014
Figure 112019118326229-pat00014

Figure 112019118326229-pat00015
Figure 112019118326229-pat00015

Figure 112019118326229-pat00016
Figure 112019118326229-pat00016

Figure 112019118326229-pat00017
Figure 112019118326229-pat00017

3. KASP 반응 조건3. KASP reaction conditions

LGC genomics(Laboratory of the Government Chemist Genomics)에서 권고한 방법으로 실시하였고, 실험에 사용한 시약은 KASP 2X master mix 및 KASP Assay mix(LGC genomics)이다. KASP 반응 조건은 하기 표 19와 같다.It was carried out by the method recommended by LGC genomics (Laboratory of the Government Chemist Genomics), and the reagents used in the experiment were KASP 2X master mix and KASP Assay mix (LGC genomics). KASP reaction conditions are shown in Table 19 below.

KASP 조건KASP conditions 단계step 온도(℃)Temperature(℃) 시간time 반복 횟수Number of repetitions Hot-start activationHot-start activation 9494 15초15 seconds 1One DenatureDenature 9494 20초20 seconds 1010 Annealing/ElongationAnnealing/Elongation 61~55
(사이클 당 0.6℃씩 감소)61~55
(Decrease by 0.6℃ per cycle) 1분1 min DenatureDenature 9494 20초20 seconds 26
26
 Annealing/ElongationAnnealing/Elongation 5757 1분1 min

실시예 1. KASP용 프라이머 세트를 이용한 참외의 유전적 배경 분석Example 1. Analysis of genetic background of melon using a primer set for KASP

30개의 참외 육성계통(농우바이오 제공)을 대상으로 본 발명의 KASP 프라이머 세트 320개를 이용하여 유전형 분석을 수행한 결과, 도 2 내지 도 7에 나타난 바와 같이 각각의 프라이머 세트를 사용한 경우 참외 육성계통 30개의 유전자형이 구별되는 것을 확인하였다.As a result of performing genotyping analysis using 320 KASP primer sets of the present invention for 30 melon breeding lines (provided by Nongwoo Bio), when using each primer set as shown in Figs. 2 to 7, melon breeding lines It was confirmed that 30 genotypes were distinguished.

이를 통해, 본 발명의 KASP 프라이머 세트는 다양한 참외의 유전적 배경을 구별하여 참외의 마커이용여교잡 육종에 유용하게 활용될 수 있을 것으로 사료되었다.Through this, it was thought that the KASP primer set of the present invention could be usefully used for hybridization using markers of melon by distinguishing the genetic background of various melon seeds.

<110> Korea Research Institute of Bioscience and Biotechnology <120> Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof <130> PN19354 <160> 960 <170> KoPatentIn 3.0 <210> 1 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 1 gaaggtgacc aagttcatgc tgccactgac cttcttcaga act 43 <210> 2 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 2 gaaggtcgga gtcaacggat tgccactgac cttcttcaga acg 43 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 ttcaggtcgc actgctcaat 20 <210> 4 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 gaaggtgacc aagttcatgc ttgtaagatt gtagaacagc ccg 43 <210> 5 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 gaaggtcgga gtcaacggat ttgtaagatt gtagaacagc cca 43 <210> 6 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 gtgcacagat atgttgacca cc 22 <210> 7 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 gaaggtgacc aagttcatgc tgacccgagt tggtgaagct ag 42 <210> 8 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 gaaggtcgga gtcaacggat tgacccgagt tggtgaagct aa 42 <210> 9 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 actgacatgt tccatagggg t 21 <210> 10 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 gaaggtgacc aagttcatgc ttgaatttca attcgagtag gctctt 46 <210> 11 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 gaaggtcgga gtcaacggat ttgaatttca attcgagtag gctctc 46 <210> 12 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 agatgagatg ttcaaggtag ctga 24 <210> 13 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 gaaggtgacc aagttcatgc ttcttttagg tttcgctaaa ctcgtc 46 <210> 14 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 gaaggtcgga gtcaacggat ttcttttagg tttcgctaaa ctcgtt 46 <210> 15 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 aagaagtcta ggagagagag ct 22 <210> 16 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 16 gaaggtgacc aagttcatgc tacacaacta cagactcgat gc 42 <210> 17 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 gaaggtcgga gtcaacggat tacacaacta cagactcgat gg 42 <210> 18 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 18 tcttgggata caaacgtgtt gtt 23 <210> 19 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 19 gaaggtgacc aagttcatgc tctccggacc gccattcag 39 <210> 20 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 20 gaaggtcgga gtcaacggat tctccggacc gccattcaa 39 <210> 21 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 21 tgatggacgc atgtgaagc 19 <210> 22 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 22 gaaggtgacc aagttcatgc tcaccatcta cctctccgat tcattc 46 <210> 23 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 23 gaaggtcgga gtcaacggat tcaccatcta cctctccgat tcatta 46 <210> 24 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 24 gacttggcaa cggaaaaggc 20 <210> 25 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 25 gaaggtgacc aagttcatgc tcctaccagc atctgaaaag gttc 44 <210> 26 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 26 gaaggtcgga gtcaacggat tcctaccagc atctgaaaag gttg 44 <210> 27 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 27 gacatgttcc tcctccagcg 20 <210> 28 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 28 gaaggtgacc aagttcatgc ttttgtacca aactcctcac tagac 45 <210> 29 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 29 gaaggtcgga gtcaacggat ttttgtacca aactcctcac tagat 45 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 30 tgtgggcttg acacactctt 20 <210> 31 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 31 gaaggtgacc aagttcatgc tagtatcgtt cggcagcatc g 41 <210> 32 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 32 gaaggtcgga gtcaacggat tagtatcgtt cggcagcatc a 41 <210> 33 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 33 attccgactt tgcaatccgc 20 <210> 34 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 34 gaaggtgacc aagttcatgc tcctttcatt ttctatggtc tttgacttc 49 <210> 35 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 35 gaaggtcgga gtcaacggat tatcctttca ttttctatgg tctttgactt t 51 <210> 36 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 36 taatgtgatg tcgacacaca cattccaat 29 <210> 37 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 37 gaaggtgacc aagttcatgc tgctgtctga agagtgccaa t 41 <210> 38 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 38 gaaggtcgga gtcaacggat tgctgtctga agagtgccaa c 41 <210> 39 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 39 tctgtcacta gttatgtcag cgt 23 <210> 40 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 40 gaaggtgacc aagttcatgc tgggtcaatc tcggtgctga t 41 <210> 41 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 41 gaaggtcgga gtcaacggat tgggtcaatc tcggtgctga c 41 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 42 tgataggatt ggccagaacg t 21 <210> 43 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 43 gaaggtgacc aagttcatgc tactgaagtg ttatgagtgg ccg 43 <210> 44 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 44 gaaggtcgga gtcaacggat tactgaagtg ttatgagtgg cca 43 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 45 caacaaggag atgcggtggt 20 <210> 46 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 46 gaaggtgacc aagttcatgc tacagatcta agccattcat gatca 45 <210> 47 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 47 gaaggtcgga gtcaacggat tacagatcta agccattcat gatcg 45 <210> 48 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 48 tggagattca tgatccagcg a 21 <210> 49 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 49 gaaggtgacc aagttcatgc tcgatggcgt cgtccttct 39 <210> 50 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 50 gaaggtcgga gtcaacggat tcgatggcgt cgtccttcc 39 <210> 51 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 51 acacatacct tactgatgcc c 21 <210> 52 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 52 gaaggtgacc aagttcatgc tctactacgc tagccaagat actc 44 <210> 53 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 53 gaaggtcgga gtcaacggat tgtctactac gctagccaag atactt 46 <210> 54 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 54 gccagtaagc tactaaacca gggta 25 <210> 55 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 55 gaaggtgacc aagttcatgc tttgacatat cgaagtcgcg ta 42 <210> 56 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 56 gaaggtcgga gtcaacggat tttgacatat cgaagtcgcg tg 42 <210> 57 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 57 agcacgcaat gtatagtcct c 21 <210> 58 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 58 gaaggtgacc aagttcatgc tttgtggtag tgttacgagt tct 43 <210> 59 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 59 gaaggtcgga gtcaacggat tttgtggtag tgttacgagt tcc 43 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 60 tggccagttc agatgatgct 20 <210> 61 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 61 gaaggtgacc aagttcatgc taataagaaa tgggagtgag agtaagg 47 <210> 62 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 62 gaaggtcgga gtcaacggat tcaataagaa atgggagtga gagtaaga 48 <210> 63 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 63 cttcctccat ggtttatttt cgtcacatt 29 <210> 64 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 64 gaaggtgacc aagttcatgc tcgcacagat tcaaaactcg acact 45 <210> 65 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 65 gaaggtcgga gtcaacggat tgcacagatt caaaactcga cacc 44 <210> 66 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 66 atgaaaatat ctagtagcga ccgaacaaaa 30 <210> 67 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 67 gaaggtgacc aagttcatgc tagcagctca gaaccatcac g 41 <210> 68 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 68 gaaggtcgga gtcaacggat tagcagctca gaaccatcac a 41 <210> 69 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 69 aggccggttt aaatggttca 20 <210> 70 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 70 gaaggtgacc aagttcatgc taccttatct ttgagtgtac cctctt 46 <210> 71 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 71 gaaggtcgga gtcaacggat taccttatct ttgagtgtac cctctc 46 <210> 72 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 72 tcttcctcat aaacaacaag tggt 24 <210> 73 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 73 gaaggtgacc aagttcatgc tactaagagg ggtggctaag tca 43 <210> 74 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 74 gaaggtcgga gtcaacggat tactaagagg ggtggctaag tcg 43 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 75 ggattttgag cccttgccca 20 <210> 76 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 76 gaaggtgacc aagttcatgc tatgcctcac cccgcacaac 40 <210> 77 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 77 gaaggtcgga gtcaacggat tatgcctcac cccgcacaat 40 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 78 agttgcacca cgtctcacat 20 <210> 79 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 79 gaaggtgacc aagttcatgc tagactatca gttaacaggc attctt 46 <210> 80 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 80 gaaggtcgga gtcaacggat tagactatca gttaacaggc attcta 46 <210> 81 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 81 tcgttcttgc ggtcttctcc 20 <210> 82 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 82 gaaggtgacc aagttcatgc tatgccgtga ctgcaaagta a 41 <210> 83 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 83 gaaggtcgga gtcaacggat tatgccgtga ctgcaaagta g 41 <210> 84 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 84 gtgctatgaa gacacaccta tca 23 <210> 85 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 85 gaaggtgacc aagttcatgc tcggacggtc atgtggaatc c 41 <210> 86 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 86 gaaggtcgga gtcaacggat tctcggacgg tcatgtggaa tca 43 <210> 87 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 87 gcctcgagat tacacttttg tgttcaat 28 <210> 88 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 88 gaaggtgacc aagttcatgc tgcccaaccc agccgtctg 39 <210> 89 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 89 gaaggtcgga gtcaacggat tgcccaaccc agccgtcta 39 <210> 90 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 90 gtgcacgtag ctggaagga 19 <210> 91 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 91 gaaggtgacc aagttcatgc tagtgctgag aaggctatga aa 42 <210> 92 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 92 gaaggtcgga gtcaacggat tagtgctgag aaggctatga ag 42 <210> 93 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 93 tcaactatca agtaagcatc ggc 23 <210> 94 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 94 gaaggtgacc aagttcatgc tgctgatggt ggttcgaaag tga 43 <210> 95 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 95 gaaggtcgga gtcaacggat tgctgatggt ggttcgaaag tgc 43 <210> 96 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 96 tcgacccaat caaatgcagc 20 <210> 97 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 97 gaaggtgacc aagttcatgc taatactccc gtaaccaccc c 41 <210> 98 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 98 gaaggtcgga gtcaacggat taatactccc gtaaccaccc t 41 <210> 99 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 99 cgaaactttc gcttcacgga 20 <210> 100 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 100 gaaggtgacc aagttcatgc tcatgtgtcc catgtcctat tgaa 44 <210> 101 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 101 gaaggtcgga gtcaacggat tcatgtgtcc catgtcctat tgag 44 <210> 102 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 102 ggccattctt aggggagaac a 21 <210> 103 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 103 gaaggtgacc aagttcatgc tcgtgtgggt taggacaaaa gca 43 <210> 104 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 104 gaaggtcgga gtcaacggat tcgtgtgggt taggacaaaa gct 43 <210> 105 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 105 agaagcttta ggtcgaaaag ga 22 <210> 106 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 106 gaaggtgacc aagttcatgc ttcataagta ctgagttaag ggtgaa 46 <210> 107 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 107 gaaggtcgga gtcaacggat ttcataagta ctgagttaag ggtgat 46 <210> 108 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 108 ggttctgcag taagctttgg c 21 <210> 109 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 109 gaaggtgacc aagttcatgc tagtccccta aagctcgtgt a 41 <210> 110 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 110 gaaggtcgga gtcaacggat tagtccccta aagctcgtgt g 41 <210> 111 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 111 tccccctttc aatttccggg 20 <210> 112 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 112 gaaggtgacc aagttcatgc tgttcaaatt cggttcctac aacttattaa a 51 <210> 113 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 113 gaaggtcgga gtcaacggat tcaaattcgg ttcctacaac ttattaag 48 <210> 114 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 114 ggatgtcgtt aaggtaattg gtcatgttt 29 <210> 115 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 115 gaaggtgacc aagttcatgc tgttctttag gggttcctac tgtcat 46 <210> 116 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 116 gaaggtcgga gtcaacggat tgttctttag gggttcctac tgtcac 46 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 117 ttcgtctcgg agcatagcca 20 <210> 118 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 118 gaaggtgacc aagttcatgc tgcagacctc cttagtattc tcact 45 <210> 119 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 119 gaaggtcgga gtcaacggat tgcagacctc cttagtattc tcacc 45 <210> 120 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 120 gaactggttc tgaaggatgt ca 22 <210> 121 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 121 gaaggtgacc aagttcatgc ttgccggcca tctttaaaga ctg 43 <210> 122 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 122 gaaggtcgga gtcaacggat ttgccggcca tctttaaaga cta 43 <210> 123 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 123 tggacctgtt tgtcgagagt g 21 <210> 124 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 124 gaaggtgacc aagttcatgc ttctcagccg tttgatccaa ta 42 <210> 125 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 125 gaaggtcgga gtcaacggat ttctcagccg tttgatccaa tg 42 <210> 126 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 126 cgtggcagag atcctctacg 20 <210> 127 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 127 gaaggtgacc aagttcatgc tcctccccat gcctttgtgt 40 <210> 128 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 128 gaaggtcgga gtcaacggat tcctccccat gcctttgtgg 40 <210> 129 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 129 tcggtattgt ccagcaagca 20 <210> 130 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 130 gaaggtgacc aagttcatgc taaagttagg cttgccttca tcatgc 46 <210> 131 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 131 gaaggtcgga gtcaacggat taaagttagg cttgccttca tcatgt 46 <210> 132 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 132 ctcgaaccca ctcgagagga cta 23 <210> 133 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 133 gaaggtgacc aagttcatgc tctgtcatcc cgtctcaatg ag 42 <210> 134 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 134 gaaggtcgga gtcaacggat tctgtcatcc cgtctcaatg aa 42 <210> 135 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 135 ctgcctacgc cctaccaaaa 20 <210> 136 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 136 gaaggtgacc aagttcatgc taggaaagga aagttaaagt cgcg 44 <210> 137 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 137 gaaggtcgga gtcaacggat taggaaagga aagttaaagt cgcc 44 <210> 138 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 138 tgattcctgt ctgattgttg ct 22 <210> 139 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 139 gaaggtgacc aagttcatgc tagtattata tgtagataat attctactca ttgg 54 <210> 140 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 140 gaaggtcgga gtcaacggat tagtattata tgtagataat attctactca ttgt 54 <210> 141 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 141 attgatatca gtaagataac gtacgacatt 30 <210> 142 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 142 gaaggtgacc aagttcatgc tagtgcattt acgggacttc a 41 <210> 143 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 143 gaaggtcgga gtcaacggat tagtgcattt acgggacttc g 41 <210> 144 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 gctccaacga tcccagagt 19 <210> 145 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 gaaggtgacc aagttcatgc tgagcacatg tcaaagcagt agtatct 47 <210> 146 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 146 gaaggtcgga gtcaacggat tagcacatgt caaagcagta gtatcc 46 <210> 147 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 cgtttaagag tctttgttgg cttagagat 29 <210> 148 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 gaaggtgacc aagttcatgc tacacaaccg aattcgagaa aag 43 <210> 149 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 149 gaaggtcgga gtcaacggat tacacaaccg aattcgagaa aaa 43 <210> 150 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 tggtaggccc gtttgtttgt 20 <210> 151 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 gaaggtgacc aagttcatgc tagtgtcgcg ccaacttatg g 41 <210> 152 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 152 gaaggtcgga gtcaacggat tagtgtcgcg ccaacttatg a 41 <210> 153 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 tcactgatgt attaacacgg ct 22 <210> 154 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 gaaggtgacc aagttcatgc tgattgaggt ctagaggctg act 43 <210> 155 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 155 gaaggtcgga gtcaacggat tgattgaggt ctagaggctg acc 43 <210> 156 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 cacccaataa gtgcggaaac a 21 <210> 157 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 gaaggtgacc aagttcatgc taggtaatca gagattgcat ccag 44 <210> 158 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 158 gaaggtcgga gtcaacggat taggtaatca gagattgcat ccat 44 <210> 159 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 ttccaacaca ggcatcacga 20 <210> 160 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 gaaggtgacc aagttcatgc tgtcttatgg tgatgttcta ggtagt 46 <210> 161 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 161 gaaggtcgga gtcaacggat tgtcttatgg tgatgttcta ggtagc 46 <210> 162 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 tcacctagtc acttgtttga ggt 23 <210> 163 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 gaaggtgacc aagttcatgc ttcttgatcc tccttagcgt gaa 43 <210> 164 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 164 gaaggtcgga gtcaacggat ttcttgatcc tccttagcgt gat 43 <210> 165 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 tcctcctcat cacgatgaag a 21 <210> 166 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 gaaggtgacc aagttcatgc tagttcgtta agtcgggcaa aac 43 <210> 167 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 167 gaaggtcgga gtcaacggat tagttcgtta agtcgggcaa aat 43 <210> 168 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 tggccaataa gtcgtcatgc 20 <210> 169 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 gaaggtgacc aagttcatgc tactgcacat aaactgttat gggtatag 48 <210> 170 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 170 gaaggtcgga gtcaacggat tataactgca cataaactgt tatgggtata t 51 <210> 171 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 aatagggaag tagggtggtc ttaacattt 29 <210> 172 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 gaaggtgacc aagttcatgc tagtccacga cagagcaaat t 41 <210> 173 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 173 gaaggtcgga gtcaacggat tagtccacga cagagcaaat c 41 <210> 174 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 actgggtctt cgtcggagat 20 <210> 175 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 gaaggtgacc aagttcatgc tggattttgt gtacatgaac tgaggg 46 <210> 176 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 176 gaaggtcgga gtcaacggat tggattttgt gtacatgaac tgagga 46 <210> 177 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 gtgagaaaca gtcaagttgg ca 22 <210> 178 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 gaaggtgacc aagttcatgc ttggtttaac atctgtgtgg aaaca 45 <210> 179 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 179 gaaggtcgga gtcaacggat ttggtttaac atctgtgtgg aaacg 45 <210> 180 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 gggaggttgg agaacgttgt 20 <210> 181 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 gaaggtgacc aagttcatgc tatgaaatca accctctcgg ag 42 <210> 182 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 182 gaaggtcgga gtcaacggat tatgaaatca accctctcgg ac 42 <210> 183 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 tgtgtccaca tgtcatagct ga 22 <210> 184 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 gaaggtgacc aagttcatgc tggaaaaaca tatggcatgc acg 43 <210> 185 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 185 gaaggtcgga gtcaacggat tggaaaaaca tatggcatgc act 43 <210> 186 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 gctgtacctg cagaatgatg a 21 <210> 187 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 gaaggtgacc aagttcatgc tgttattgct gatcgtaagc atcaaac 47 <210> 188 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 188 gaaggtcgga gtcaacggat tatgttattg ctgatcgtaa gcatcaaat 49 <210> 189 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 ttaaagacat gagtccaaca tcccatgaa 29 <210> 190 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 gaaggtgacc aagttcatgc tgctgtcgtt gatgttccca at 42 <210> 191 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 191 gaaggtcgga gtcaacggat tgctgtcgtt gatgttccca ac 42 <210> 192 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 tgctgtgctg gttccatgtt 20 <210> 193 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 gaaggtgacc aagttcatgc tggattatgt tcggttgaag gcg 43 <210> 194 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 194 gaaggtcgga gtcaacggat tggattatgt tcggttgaag gcc 43 <210> 195 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 gtcggatcat tgaaagggca t 21 <210> 196 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 gaaggtgacc aagttcatgc tgcgagtaga ctatcacgca gtc 43 <210> 197 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 197 gaaggtcgga gtcaacggat tgcgagtaga ctatcacgca gtt 43 <210> 198 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 atgcgccacc tgtctttgta 20 <210> 199 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 gaaggtgacc aagttcatgc tagcattagg agtggtgtgt aataat 46 <210> 200 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 200 gaaggtcgga gtcaacggat tagcattagg agtggtgtgt aataac 46 <210> 201 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 cccagtgtca catgtccctc 20 <210> 202 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 gaaggtgacc aagttcatgc tcaaaagata caaagctcta cctcgg 46 <210> 203 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 203 gaaggtcgga gtcaacggat tcaaaagata caaagctcta cctcga 46 <210> 204 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 204 tggttccttg ctgaagaggt 20 <210> 205 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 205 gaaggtgacc aagttcatgc tgcagctcgt aaccggaaat tc 42 <210> 206 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 206 gaaggtcgga gtcaacggat tgcagctcgt aaccggaaat tt 42 <210> 207 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 207 ctctttcacc ggagagctca a 21 <210> 208 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 208 gaaggtgacc aagttcatgc ttcaaagttc ccattgctca caag 44 <210> 209 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 209 gaaggtcgga gtcaacggat ttcaaagttc ccattgctca caaa 44 <210> 210 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 210 acctgtggta ttttgccgtg a 21 <210> 211 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 211 gaaggtgacc aagttcatgc ttcattctat acactggtta gccac 45 <210> 212 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 212 gaaggtcgga gtcaacggat ttcattctat acactggtta gccaa 45 <210> 213 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 213 aaagaagtgg ccccctttgg 20 <210> 214 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 214 gaaggtgacc aagttcatgc tgaatgtcat aagtcgtatt cacacg 46 <210> 215 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 215 gaaggtcgga gtcaacggat tgaatgtcat aagtcgtatt cacaca 46 <210> 216 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 216 gcttgcaaaa ctagacaaca cg 22 <210> 217 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 217 gaaggtgacc aagttcatgc tggttctaag gatgctgtgg gttc 44 <210> 218 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 218 gaaggtcgga gtcaacggat tggttctaag gatgctgtgg gtta 44 <210> 219 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 219 aaaatgggtc aaagccttgc tc 22 <210> 220 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 220 gaaggtgacc aagttcatgc tgaagaaacg ttgcacccaa taa 43 <210> 221 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 221 gaaggtcgga gtcaacggat tgaagaaacg ttgcacccaa tag 43 <210> 222 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 222 aggtggaatc cagatgctat tgt 23 <210> 223 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 223 gaaggtgacc aagttcatgc tccccgatta taacaggttg cacc 44 <210> 224 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 224 gaaggtcgga gtcaacggat taccccgatt ataacaggtt gcaca 45 <210> 225 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 225 gtcggtggtt attatagttt gtcaggtat 29 <210> 226 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 gaaggtgacc aagttcatgc tagaaacctg atgcagactg at 42 <210> 227 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 227 gaaggtcgga gtcaacggat tagaaacctg atgcagactg ac 42 <210> 228 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 agttgcaact cctggtcgtt 20 <210> 229 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 gaaggtgacc aagttcatgc tgtagtcata attgcgctgt taacg 45 <210> 230 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 230 gaaggtcgga gtcaacggat tgtagtcata attgcgctgt taaca 45 <210> 231 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 231 aaagagagtt agacttccat cgt 23 <210> 232 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 232 gaaggtgacc aagttcatgc tgaaatctaa acaagaaaga ttcatatcct c 51 <210> 233 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 233 gaaggtcgga gtcaacggat tctgaaatct aaacaagaaa gattcatatc ctt 53 <210> 234 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 234 ccaccttcat ctatggtgct tgcta 25 <210> 235 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 235 gaaggtgacc aagttcatgc tgtgtttagc ataatcgtga ccgc 44 <210> 236 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 236 gaaggtcgga gtcaacggat tgtgtttagc ataatcgtga ccgt 44 <210> 237 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 237 aggcttgtgt gactctcgc 19 <210> 238 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 238 gaaggtgacc aagttcatgc tgaacaaaac tccaccggtg g 41 <210> 239 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 239 gaaggtcgga gtcaacggat tgaacaaaac tccaccggtg t 41 <210> 240 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 240 gacgccggag ataagggtag 20 <210> 241 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 241 gaaggtgacc aagttcatgc tcaatcttca ataacgcggc ct 42 <210> 242 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 242 gaaggtcgga gtcaacggat tcaatcttca ataacgcggc cg 42 <210> 243 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 243 ttcctctact tcctccgcga 20 <210> 244 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 244 gaaggtgacc aagttcatgc tggacacatg cctcattagc ga 42 <210> 245 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 245 gaaggtcgga gtcaacggat tggacacatg cctcattagc gg 42 <210> 246 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 246 gcgtacgagc tagaattcct 20 <210> 247 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 247 gaaggtgacc aagttcatgc ttttccggtg cccctcctta a 41 <210> 248 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 248 gaaggtcgga gtcaacggat ttttccggtg cccctcctta c 41 <210> 249 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 249 agctcaactc gagagggagg 20 <210> 250 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 250 gaaggtgacc aagttcatgc tgccaatcta gtgagcatgg ga 42 <210> 251 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 251 gaaggtcgga gtcaacggat tgccaatcta gtgagcatgg gg 42 <210> 252 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 252 gcaaattctg cggttcgtgc 20 <210> 253 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 253 gaaggtgacc aagttcatgc tctgtctgga caattatgcg ca 42 <210> 254 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 254 gaaggtcgga gtcaacggat tctgtctgga caattatgcg ct 42 <210> 255 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 255 tggatcccat gaggtaccca 20 <210> 256 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 256 gaaggtgacc aagttcatgc ttctacaggc ttcggcaatc a 41 <210> 257 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 257 gaaggtcgga gtcaacggat ttctacaggc ttcggcaatc g 41 <210> 258 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 258 gcccctactt gaagttcggt 20 <210> 259 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 259 gaaggtgacc aagttcatgc tgaatcaact tgttcaaaac cgacacg 47 <210> 260 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 260 gaaggtcgga gtcaacggat tgaatcaact tgttcaaaac cgacaca 47 <210> 261 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 261 gggaaagttc agctcaaaag catcataa 28 <210> 262 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 262 gaaggtgacc aagttcatgc tgcctacttc tgaacaactt gcg 43 <210> 263 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 263 gaaggtcgga gtcaacggat tgcctacttc tgaacaactt gca 43 <210> 264 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 264 tgcatacttc acgctcagct 20 <210> 265 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 265 gaaggtgacc aagttcatgc tttttcgaag gaggccgcgg 40 <210> 266 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 266 gaaggtcgga gtcaacggat tttttcgaag gaggccgcga 40 <210> 267 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 267 acgcccgtaa gagttcactc 20 <210> 268 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 268 gaaggtgacc aagttcatgc tccgtcgcct ccccctacc 39 <210> 269 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 269 gaaggtcgga gtcaacggat tccgtcgcct ccccctact 39 <210> 270 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 270 tcaaaacgag gcaaggacgt 20 <210> 271 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 271 gaaggtgacc aagttcatgc tgtaatccag attatcctct cccaaa 46 <210> 272 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 272 gaaggtcgga gtcaacggat tgtaatccag attatcctct cccaag 46 <210> 273 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 273 aggctgttaa acattaaggg gt 22 <210> 274 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 274 gaaggtgacc aagttcatgc tcttcaactt tttgggggca ct 42 <210> 275 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 275 gaaggtcgga gtcaacggat tcttcaactt tttgggggca ca 42 <210> 276 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 276 tatgaacggc gggagtttcc 20 <210> 277 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 277 gaaggtgacc aagttcatgc tatggccatg ataaaagacc atcagg 46 <210> 278 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 278 gaaggtcgga gtcaacggat tgatggccat gataaaagac catcaga 47 <210> 279 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 279 gtcatgattg tccagataac cacgtttt 28 <210> 280 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 280 gaaggtgacc aagttcatgc tacacgattt tcctctctag gcc 43 <210> 281 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 281 gaaggtcgga gtcaacggat tacacgattt tcctctctag gct 43 <210> 282 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 282 cgcgtaccaa aggaaatcgc 20 <210> 283 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 283 gaaggtgacc aagttcatgc tgaatcaagc taagcatggt ctgaac 46 <210> 284 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 284 gaaggtcgga gtcaacggat taagaatcaa gctaagcatg gtctgaaa 48 <210> 285 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 285 cggctcctat ttgacacttt atacatgta 29 <210> 286 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 286 gaaggtgacc aagttcatgc tgggaagtgg gattagggat ca 42 <210> 287 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 287 gaaggtcgga gtcaacggat tgggaagtgg gattagggat cg 42 <210> 288 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 288 tcaggccatc tgatctacat gat 23 <210> 289 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 289 gaaggtgacc aagttcatgc tccttctgaa tcaaatgctt agacct 46 <210> 290 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 290 gaaggtcgga gtcaacggat tccttctgaa tcaaatgctt agaccc 46 <210> 291 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 291 tttagttgac atcatgcccc c 21 <210> 292 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 292 gaaggtgacc aagttcatgc taggcttgac gtggtggatt t 41 <210> 293 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 293 gaaggtcgga gtcaacggat taggcttgac gtggtggatt c 41 <210> 294 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 294 tgtgtagtgt tcttccgcgt 20 <210> 295 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 295 gaaggtgacc aagttcatgc ttgaggagtt tccaaaatca acgg 44 <210> 296 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 296 gaaggtcgga gtcaacggat ttgaggagtt tccaaaatca acgc 44 <210> 297 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 297 cagcttccaa agagaggacg a 21 <210> 298 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 298 gaaggtgacc aagttcatgc tcaagggtat tgtccagttt cttagc 46 <210> 299 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 299 gaaggtcgga gtcaacggat tcaagggtat tgtccagttt cttagt 46 <210> 300 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 300 agtacgaaga attgggggcc 20 <210> 301 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 301 gaaggtgacc aagttcatgc tacttgatga gcccaacact tt 42 <210> 302 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 302 gaaggtcgga gtcaacggat tacttgatga gcccaacact tc 42 <210> 303 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 303 cgtgttagtt tgagaggaca ca 22 <210> 304 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 304 gaaggtgacc aagttcatgc ttgacttcga tacaaatggc agacta 46 <210> 305 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 305 gaaggtcgga gtcaacggat ttgacttcga tacaaatggc agactg 46 <210> 306 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 306 acatgacctc gccgctatac 20 <210> 307 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 307 gaaggtgacc aagttcatgc tgccacgctt gttggagatg ac 42 <210> 308 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 308 gaaggtcgga gtcaacggat tggccacgct tgttggagat gat 43 <210> 309 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 309 tctccacttc ctccgaaaat aactagttt 29 <210> 310 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 310 gaaggtgacc aagttcatgc tggaggtgac ggtgatcgcg 40 <210> 311 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 311 gaaggtcgga gtcaacggat tggaggtgac ggtgatcgca 40 <210> 312 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 312 gcgccttcgt ctctcctatc 20 <210> 313 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 313 gaaggtgacc aagttcatgc tgatcgatac acagatgtac atggt 45 <210> 314 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 314 gaaggtcgga gtcaacggat tgatcgatac acagatgtac atggc 45 <210> 315 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 315 gtctggctat catgacaaaa gca 23 <210> 316 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 316 gaaggtgacc aagttcatgc tagaaacttg ccaacccttt aacagata 48 <210> 317 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 317 gaaggtcgga gtcaacggat tgaaacttgc caacccttta acagatg 47 <210> 318 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 318 tccagaactt cttaagaaat gacagagaaa 30 <210> 319 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 319 gaaggtgacc aagttcatgc tggggcgcca aagagaggt 39 <210> 320 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 320 gaaggtcgga gtcaacggat tggggcgcca aagagaggc 39 <210> 321 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 321 aggtaggcgt cccctagttt 20 <210> 322 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 322 gaaggtgacc aagttcatgc ttttagaaat gattctcctc tgaccc 46 <210> 323 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 323 gaaggtcgga gtcaacggat tgttttagaa atgattctcc tctgacca 48 <210> 324 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 324 ctctcttctt tccctaaata ttaattgatt 30 <210> 325 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 325 gaaggtgacc aagttcatgc ttctttgctc tcttgactct tacac 45 <210> 326 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 326 gaaggtcgga gtcaacggat ttctttgctc tcttgactct tacat 45 <210> 327 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 327 tcctctattg aaggtcaatg gga 23 <210> 328 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 328 gaaggtgacc aagttcatgc taattcaggt acatactgcc acc 43 <210> 329 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 329 gaaggtcgga gtcaacggat taattcaggt acatactgcc act 43 <210> 330 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 330 tcgatcgttt ctcaacagcc 20 <210> 331 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 331 gaaggtgacc aagttcatgc ttgaagaaac cattgctctc tcg 43 <210> 332 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 332 gaaggtcgga gtcaacggat ttgaagaaac cattgctctc tca 43 <210> 333 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 333 ctcgaatcga gctctcatag aa 22 <210> 334 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 334 gaaggtgacc aagttcatgc tgtgtttaag ttgactacaa aaaccctca 49 <210> 335 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 335 gaaggtcgga gtcaacggat tgtttaagtt gactacaaaa accctcg 47 <210> 336 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 336 ggttatattt gttaggtata tgtattagtt 30 <210> 337 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 337 gaaggtgacc aagttcatgc tgcttacttt tagggattca aactgc 46 <210> 338 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 338 gaaggtcgga gtcaacggat tgcttacttt tagggattca aactgt 46 <210> 339 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 339 gggagagcgt tgtagcagtt 20 <210> 340 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 340 gaaggtgacc aagttcatgc tatgcctgat acatgaccca ag 42 <210> 341 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 341 gaaggtcgga gtcaacggat tatgcctgat acatgaccca aa 42 <210> 342 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 342 tcttagctcc cataaattgc ca 22 <210> 343 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 343 gaaggtgacc aagttcatgc tggaagaggg gtcagccgt 39 <210> 344 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 344 gaaggtcgga gtcaacggat tggaagaggg gtcagccgc 39 <210> 345 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 345 acgctagaaa ccgtcacacc 20 <210> 346 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 346 gaaggtgacc aagttcatgc ttttgctttt agatattcgc tggtac 46 <210> 347 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 347 gaaggtcgga gtcaacggat ttttgctttt agatattcgc tggtat 46 <210> 348 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 348 cttcgagtcg ggcagcttag 20 <210> 349 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 349 gaaggtgacc aagttcatgc tttgaaatat gccatattcg ccg 43 <210> 350 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 350 gaaggtcgga gtcaacggat tttgaaatat gccatattcg ccc 43 <210> 351 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 351 acatcaaaga ggtcgtactg aaa 23 <210> 352 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 352 gaaggtgacc aagttcatgc ttacccgccc attagttttc ccc 43 <210> 353 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 353 gaaggtcgga gtcaacggat ttacccgccc attagttttc cct 43 <210> 354 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 354 actttacttc tactcgtttg tcaga 25 <210> 355 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 355 gaaggtgacc aagttcatgc tgcgacacca gagacgactg g 41 <210> 356 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 356 gaaggtcgga gtcaacggat tgcgacacca gagacgactg a 41 <210> 357 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 357 cacctctgac tcttcgtgcc 20 <210> 358 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 358 gaaggtgacc aagttcatgc taaattttag ggaacgtatg gtttga 46 <210> 359 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 359 gaaggtcgga gtcaacggat taaattttag ggaacgtatg gtttgg 46 <210> 360 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 360 acctaaagaa accagtgacc ct 22 <210> 361 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 361 gaaggtgacc aagttcatgc tatgtggacc aagaatgccc c 41 <210> 362 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 362 gaaggtcgga gtcaacggat tatgtggacc aagaatgccc t 41 <210> 363 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 363 ctcgggacct tcacattgag t 21 <210> 364 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 364 gaaggtgacc aagttcatgc tatgtccatg caacactaaa gccc 44 <210> 365 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 365 gaaggtcgga gtcaacggat tgtatgtcca tgcaacacta aagcct 46 <210> 366 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 366 tcactaaaga gagtggactt tgagttgtt 29 <210> 367 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 367 gaaggtgacc aagttcatgc ttgttgatac cgtcccaaag gttg 44 <210> 368 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 368 gaaggtcgga gtcaacggat ttgttgatac cgtcccaaag gtta 44 <210> 369 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 369 agcgaccgat ctcacgattt 20 <210> 370 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 370 gaaggtgacc aagttcatgc tagtatgcac atcacccacc g 41 <210> 371 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 371 gaaggtcgga gtcaacggat tagtatgcac atcacccacc a 41 <210> 372 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 372 agctgagtca ctggaggcta 20 <210> 373 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 373 gaaggtgacc aagttcatgc tacaaaccag gtaagaagga gtc 43 <210> 374 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 374 gaaggtcgga gtcaacggat tacaaaccag gtaagaagga gtt 43 <210> 375 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 375 tcaaacccct catctggagc 20 <210> 376 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 376 gaaggtgacc aagttcatgc tacagttgca ggaaggaagt c 41 <210> 377 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 377 gaaggtcgga gtcaacggat tacagttgca ggaaggaagt g 41 <210> 378 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 378 ggtttccgcc acttagagga 20 <210> 379 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 379 gaaggtgacc aagttcatgc taaagaacgg gttggtaagt gc 42 <210> 380 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 380 gaaggtcgga gtcaacggat taaagaacgg gttggtaagt gt 42 <210> 381 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 381 tggccttatc aatctcggcg 20 <210> 382 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 382 gaaggtgacc aagttcatgc ttgacaaccg agttcctccc a 41 <210> 383 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 383 gaaggtcgga gtcaacggat ttgacaaccg agttcctccc g 41 <210> 384 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 384 agcatcagtc aattgtgtgt ga 22 <210> 385 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 385 gaaggtgacc aagttcatgc tcccctcccc acaacaaact 40 <210> 386 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 386 gaaggtcgga gtcaacggat tcccctcccc acaacaaacc 40 <210> 387 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 387 ggtcgtcaag gtggtttttc g 21 <210> 388 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 388 gaaggtgacc aagttcatgc tctcgatgtg ttattccact tgc 43 <210> 389 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 389 gaaggtcgga gtcaacggat tcctctcgat gtgttattcc acttgt 46 <210> 390 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 390 tggtatgaca gggcagccag ca 22 <210> 391 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 391 gaaggtgacc aagttcatgc tttttatgtc ttagggctcg aagt 44 <210> 392 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 392 gaaggtcgga gtcaacggat tttttatgtc ttagggctcg aagc 44 <210> 393 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 393 ttgtgagaca gcccaatcgg 20 <210> 394 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 394 gaaggtgacc aagttcatgc tcattaggtt tcaccccgat ca 42 <210> 395 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 395 gaaggtcgga gtcaacggat tcattaggtt tcaccccgat cg 42 <210> 396 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 396 ctggaggacc cgatcaacac 20 <210> 397 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 397 gaaggtgacc aagttcatgc tgatgtctcg taggaagact cgg 43 <210> 398 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 398 gaaggtcgga gtcaacggat tagatgtctc gtaggaagac tcga 44 <210> 399 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 399 tgtgggattt catacagatg cctaatgat 29 <210> 400 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 400 gaaggtgacc aagttcatgc tgagggaaag cggattgaca g 41 <210> 401 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 401 gaaggtcgga gtcaacggat tgagggaaag cggattgaca a 41 <210> 402 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 402 gaaagggaaa gatcgaatgc g 21 <210> 403 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 403 gaaggtgacc aagttcatgc tcgtcattga cttcgcttgt cat 43 <210> 404 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 404 gaaggtcgga gtcaacggat tcgtcattga cttcgcttgt cag 43 <210> 405 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 405 ggatatggtc gtctgaaacc ga 22 <210> 406 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 406 gaaggtgacc aagttcatgc tcgaaggtat tgcgcttaat tca 43 <210> 407 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 407 gaaggtcgga gtcaacggat tcgaaggtat tgcgcttaat tcg 43 <210> 408 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 408 gctccattga tgttctaaac ctagc 25 <210> 409 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 409 gaaggtgacc aagttcatgc tccacggaat ggcaagtctg g 41 <210> 410 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 410 gaaggtcgga gtcaacggat tccacggaat ggcaagtctg a 41 <210> 411 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 411 aagattgtca tcttgagcct aaatt 25 <210> 412 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 412 gaaggtgacc aagttcatgc tcatgtgcga cgttagtgac tatg 44 <210> 413 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 413 gaaggtcgga gtcaacggat tcatgtgcga cgttagtgac tata 44 <210> 414 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 414 acctcttggc ccaacatagc 20 <210> 415 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 415 gaaggtgacc aagttcatgc tggcttccac gaccaaggat g 41 <210> 416 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 416 gaaggtcgga gtcaacggat tggcttccac gaccaaggat t 41 <210> 417 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 417 acccccaacg tatttaggac ac 22 <210> 418 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 418 gaaggtgacc aagttcatgc tagactagct gccatagtaa gtaaag 46 <210> 419 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 419 gaaggtcgga gtcaacggat tagactagct gccatagtaa gtaaac 46 <210> 420 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 420 gtcagctcgg ttgtactaag ct 22 <210> 421 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 421 gaaggtgacc aagttcatgc tggaaaaatg gcgatgtgac gg 42 <210> 422 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 422 gaaggtcgga gtcaacggat tggaaaaatg gcgatgtgac ga 42 <210> 423 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 423 gagaaatgat tcgtccgcgc 20 <210> 424 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 424 gaaggtgacc aagttcatgc taactaagtc gccattcaca gg 42 <210> 425 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 425 gaaggtcgga gtcaacggat taactaagtc gccattcaca ga 42 <210> 426 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 426 tggggtcagc acaattagga t 21 <210> 427 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 427 gaaggtgacc aagttcatgc tgtacgactc ggagacaggt t 41 <210> 428 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 428 gaaggtcgga gtcaacggat tgtacgactc ggagacaggt c 41 <210> 429 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 429 acactttgcg ttgcaccatc 20 <210> 430 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 430 gaaggtgacc aagttcatgc tatccgtacc gtggtgatag a 41 <210> 431 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 431 gaaggtcgga gtcaacggat tatccgtacc gtggtgatag g 41 <210> 432 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 432 aaactcttct tccccaacgc t 21 <210> 433 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 433 gaaggtgacc aagttcatgc tctcaccggt gggagcaca 39 <210> 434 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 434 gaaggtcgga gtcaacggat tctcaccggt gggagcacc 39 <210> 435 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 435 gcccgcaccc gaaaaatg 18 <210> 436 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 436 gaaggtgacc aagttcatgc tctcactcgc gtgtgtaact a 41 <210> 437 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 437 gaaggtcgga gtcaacggat tctcactcgc gtgtgtaact g 41 <210> 438 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 438 atgttccact ccacatgcga 20 <210> 439 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 439 gaaggtgacc aagttcatgc taggatttcg aagtcatacc cg 42 <210> 440 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 440 gaaggtcgga gtcaacggat taggatttcg aagtcatacc cc 42 <210> 441 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 441 ctgaccagaa ggcttggaaa t 21 <210> 442 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 442 gaaggtgacc aagttcatgc tacatctctt cagcaagtag tgt 43 <210> 443 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 443 gaaggtcgga gtcaacggat tacatctctt cagcaagtag tgc 43 <210> 444 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 444 attagtaact gctaactgga ggtaa 25 <210> 445 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 445 gaaggtgacc aagttcatgc ttccttcatg acagtggaac cat 43 <210> 446 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 446 gaaggtcgga gtcaacggat ttccttcatg acagtggaac cac 43 <210> 447 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 447 tggggagaat gtatactaac tgc 23 <210> 448 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 448 gaaggtgacc aagttcatgc tgatgtgtat gttcatatag gaattgtcg 49 <210> 449 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 449 gaaggtcgga gtcaacggat tatgatgtgt atgttcatat aggaattgtc a 51 <210> 450 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 450 tttccataaa gttgctcgat tgcaaggat 29 <210> 451 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 451 gaaggtgacc aagttcatgc tgctgctctt agtagtcctg ataata 46 <210> 452 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 452 gaaggtcgga gtcaacggat tgctgctctt agtagtcctg ataatg 46 <210> 453 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 453 cggctggggg attcgtttat 20 <210> 454 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 454 gaaggtgacc aagttcatgc tactgacgaa agcagccaga g 41 <210> 455 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 455 gaaggtcgga gtcaacggat tactgacgaa agcagccaga c 41 <210> 456 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 456 ctgaccgtaa ccgactctgt 20 <210> 457 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 457 gaaggtgacc aagttcatgc tgtccacctc ccctcaccg 39 <210> 458 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 458 gaaggtcgga gtcaacggat tgtccacctc ccctcacca 39 <210> 459 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 459 gggtgctttg caataacagg a 21 <210> 460 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 460 gaaggtgacc aagttcatgc ttgatttgat gtaagggttg actagg 46 <210> 461 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 461 gaaggtcgga gtcaacggat ttgatttgat gtaagggttg actaga 46 <210> 462 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 462 accaatacag ttagacccaa tga 23 <210> 463 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 463 gaaggtgacc aagttcatgc tggttgattc ttacagtttc cgct 44 <210> 464 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 464 gaaggtcgga gtcaacggat tggttgattc ttacagtttc cgcc 44 <210> 465 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 465 cgtttctcta atccccaccc a 21 <210> 466 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 466 gaaggtgacc aagttcatgc tcataagtgc tgcctcctgg ta 42 <210> 467 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 467 gaaggtcgga gtcaacggat tcataagtgc tgcctcctgg tg 42 <210> 468 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 468 gacagaagct aaaactctca gcc 23 <210> 469 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 469 gaaggtgacc aagttcatgc tcacgcgtca tcatcacgtt g 41 <210> 470 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 470 gaaggtcgga gtcaacggat tcacgcgtca tcatcacgtt t 41 <210> 471 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 471 caccatcgat gatcacatgc a 21 <210> 472 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 472 gaaggtgacc aagttcatgc ttgtccaatt ggcggctgta c 41 <210> 473 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 473 gaaggtcgga gtcaacggat ttgtccaatt ggcggctgta a 41 <210> 474 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 474 gcttttgtcc atttagtccc ca 22 <210> 475 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 475 gaaggtgacc aagttcatgc tgccctatag gatcaatcac actacc 46 <210> 476 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 476 gaaggtcgga gtcaacggat tgccctatag gatcaatcac actact 46 <210> 477 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 477 actcctcgtg gttcacaaga 20 <210> 478 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 478 gaaggtgacc aagttcatgc tccctagctt ccgagacaga t 41 <210> 479 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 479 gaaggtcgga gtcaacggat tccctagctt ccgagacaga a 41 <210> 480 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 480 tggattgatc ggatggggtg 20 <210> 481 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 481 gaaggtgacc aagttcatgc tgcaaatgct agacatatcc cgac 44 <210> 482 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 482 gaaggtcgga gtcaacggat tgcaaatgct agacatatcc cgat 44 <210> 483 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 483 acgtcgtgtc gagagatctt 20 <210> 484 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 484 gaaggtgacc aagttcatgc taccccgtac agtatgtggc c 41 <210> 485 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 485 gaaggtcgga gtcaacggat tgtaccccgt acagtatgtg gca 43 <210> 486 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 486 ttctatggtg gcaaattgca aggaagtaa 29 <210> 487 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 487 gaaggtgacc aagttcatgc tgcaaaccag gtccacgcaa 40 <210> 488 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 488 gaaggtcgga gtcaacggat tgcaaaccag gtccacgcag 40 <210> 489 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 489 atggtggacg tgcttctcag 20 <210> 490 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 490 gaaggtgacc aagttcatgc tccacgtggc agcattggag 40 <210> 491 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 491 gaaggtcgga gtcaacggat tccacgtggc agcattggaa 40 <210> 492 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 492 taatctgccc ccgaggtgta 20 <210> 493 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 493 gaaggtgacc aagttcatgc tgggccacac gattcctgc 39 <210> 494 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 494 gaaggtcgga gtcaacggat tgggccacac gattcctgg 39 <210> 495 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 495 tgaatgtgga gcctcatgtc a 21 <210> 496 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 496 gaaggtgacc aagttcatgc taagaacctc aaaacttttg tatactcaaa c 51 <210> 497 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 497 gaaggtcgga gtcaacggat tgaaagaacc tcaaaacttt tgtatactca aat 53 <210> 498 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 498 cgcttatcaa agcttgcctc gacta 25 <210> 499 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 499 gaaggtgacc aagttcatgc tctgttcatc gacgacggca g 41 <210> 500 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 500 gaaggtcgga gtcaacggat tctgttcatc gacgacggca a 41 <210> 501 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 501 tcctagccgt ccctcgatat 20 <210> 502 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 502 gaaggtgacc aagttcatgc tcatgcgatc aattcccaac ca 42 <210> 503 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 503 gaaggtcgga gtcaacggat tcatgcgatc aattcccaac cg 42 <210> 504 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 504 tgtctgtcat ctttgttccc ct 22 <210> 505 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 505 gaaggtgacc aagttcatgc tcttatgatt gccaacaata ttcctgt 47 <210> 506 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 506 gaaggtcgga gtcaacggat tcttatgatt gccaacaata ttcctgc 47 <210> 507 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 507 caaaagctta aattagtggg tgaaggcaa 29 <210> 508 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 508 gaaggtgacc aagttcatgc tgccgcccaa gttcaactcc 40 <210> 509 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 509 gaaggtcgga gtcaacggat tgccgcccaa gttcaactct 40 <210> 510 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 510 cggttgactt tgacggcaag 20 <210> 511 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 511 gaaggtgacc aagttcatgc tagacgccaa atcacgagac g 41 <210> 512 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 512 gaaggtcgga gtcaacggat tagacgccaa atcacgagac t 41 <210> 513 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 513 ggattaggtc gtttggcagt 20 <210> 514 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 514 gaaggtgacc aagttcatgc ttcgtattgt tcatatttgc agatcg 46 <210> 515 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 515 gaaggtcgga gtcaacggat ttcgtattgt tcatatttgc agatca 46 <210> 516 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 516 acagggagga ttaaacttgt ga 22 <210> 517 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 517 gaaggtgacc aagttcatgc tccaaccgtg agacattgag ct 42 <210> 518 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 518 gaaggtcgga gtcaacggat tccaaccgtg agacattgag cc 42 <210> 519 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 519 actaacatgg tgcactttca ct 22 <210> 520 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 520 gaaggtgacc aagttcatgc tggatttgct cgatgtcaaa gcg 43 <210> 521 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 521 gaaggtcgga gtcaacggat tggatttgct cgatgtcaaa gca 43 <210> 522 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 522 atacttccga ccctcatcgc 20 <210> 523 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 523 gaaggtgacc aagttcatgc tagacacgtt gccatgagtt at 42 <210> 524 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 524 gaaggtcgga gtcaacggat tagacacgtt gccatgagtt aa 42 <210> 525 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 525 tcgtattgtt cggtaagcta cc 22 <210> 526 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 526 gaaggtgacc aagttcatgc tgttgccgct ctgctcgatc 40 <210> 527 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 527 gaaggtcgga gtcaacggat tgttgccgct ctgctcgata 40 <210> 528 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 528 gagccaatct gagtgacgag t 21 <210> 529 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 529 gaaggtgacc aagttcatgc tcgagcttgt ctggcgatcg 40 <210> 530 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 530 gaaggtcgga gtcaacggat tcgagcttgt ctggcgatca 40 <210> 531 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 531 tcaataacta cgatcgccag aca 23 <210> 532 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 532 gaaggtgacc aagttcatgc tagctaactg acggtccggg 40 <210> 533 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 533 gaaggtcgga gtcaacggat tagctaactg acggtccgga 40 <210> 534 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 534 acaatcttac ttgcgcagga 20 <210> 535 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 535 gaaggtgacc aagttcatgc tgcttatgac caacgcgcca t 41 <210> 536 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 536 gaaggtcgga gtcaacggat tgcttatgac caacgcgcca g 41 <210> 537 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 537 gggttaaaaa gcggtcgtgg 20 <210> 538 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 538 gaaggtgacc aagttcatgc tgttagttaa tcttaaatcg ttggagctca 50 <210> 539 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 539 gaaggtcgga gtcaacggat tagttaatct taaatcgttg gagctcg 47 <210> 540 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 540 tagtgtggga acctaatgga ccaataaat 29 <210> 541 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 541 gaaggtgacc aagttcatgc ttcaccattt gcagacgacg c 41 <210> 542 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 542 gaaggtcgga gtcaacggat ttcaccattt gcagacgacg t 41 <210> 543 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 543 agcttttgtt cacatgcgag g 21 <210> 544 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 544 gaaggtgacc aagttcatgc taatgggtta tcttcaacaa ctattaaaag c 51 <210> 545 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 545 gaaggtcgga gtcaacggat taaaatgggt tatcttcaac aactattaaa agt 53 <210> 546 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 546 gagtacatgc caaacactac aggaaataa 29 <210> 547 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 547 gaaggtgacc aagttcatgc ttgagaggat tcccattggc tc 42 <210> 548 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 548 gaaggtcgga gtcaacggat ttgagaggat tcccattggc tt 42 <210> 549 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 549 aagggcttgt tccttctcga 20 <210> 550 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 550 gaaggtgacc aagttcatgc tggtcgttgg tgtcaattga ct 42 <210> 551 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 551 gaaggtcgga gtcaacggat tggtcgttgg tgtcaattga cc 42 <210> 552 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 552 gaggtggata ttgtagaagg gca 23 <210> 553 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 553 gaaggtgacc aagttcatgc tgaatcaatg tgaagcggag taatgt 46 <210> 554 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 554 gaaggtcgga gtcaacggat tgaatcaatg tgaagcggag taatgg 46 <210> 555 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 555 cgacctgtcg ttcagcgt 18 <210> 556 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 556 gaaggtgacc aagttcatgc ttccaactca ctcaattgtg acttaca 47 <210> 557 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 557 gaaggtcgga gtcaacggat tccaactcac tcaattgtga cttacc 46 <210> 558 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 558 gcctccaagt gttgagacgt acaaa 25 <210> 559 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 559 gaaggtgacc aagttcatgc taaaaacagc tttcgtcagt ctcg 44 <210> 560 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 560 gaaggtcgga gtcaacggat taaaaacagc tttcgtcagt ctca 44 <210> 561 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 561 tttgtttcga tccattctga cca 23 <210> 562 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 562 gaaggtgacc aagttcatgc ttcgaagagg taggttgcgg a 41 <210> 563 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 563 gaaggtcgga gtcaacggat ttcgaagagg taggttgcgg g 41 <210> 564 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 564 ctttcctctt gccttgggga 20 <210> 565 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 565 gaaggtgacc aagttcatgc taggagaata gggtcgactg tcc 43 <210> 566 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 566 gaaggtcgga gtcaacggat taaaaggaga atagggtcga ctgtct 46 <210> 567 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 567 caaatcactc ttcaaagagc aagagcatt 29 <210> 568 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 568 gaaggtgacc aagttcatgc tctacgcagc ccaaaaacgt ag 42 <210> 569 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 569 gaaggtcgga gtcaacggat tctacgcagc ccaaaaacgt at 42 <210> 570 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 570 cgacactgtt gttgtgcgtt 20 <210> 571 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 571 gaaggtgacc aagttcatgc taacttacac ttgaagctat ggtatg 46 <210> 572 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 572 gaaggtcgga gtcaacggat taacttacac ttgaagctat ggtatc 46 <210> 573 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 573 gaaatggaat gattgcttca ctct 24 <210> 574 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 574 gaaggtgacc aagttcatgc tcataccatc tagttggtgc atctg 45 <210> 575 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 575 gaaggtcgga gtcaacggat tgcataccat ctagttggtg catcta 46 <210> 576 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 576 cagaaaacaa aggcctacag ctattgaaa 29 <210> 577 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 577 gaaggtgacc aagttcatgc tttggctcct cgcggatgc 39 <210> 578 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 578 gaaggtcgga gtcaacggat tttggctcct cgcggatgt 39 <210> 579 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 579 tggatcctat ttccgcttag ca 22 <210> 580 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 580 gaaggtgacc aagttcatgc tgttgtcctc tatcttcgtt gct 43 <210> 581 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 581 gaaggtcgga gtcaacggat tgttgtcctc tatcttcgtt gcc 43 <210> 582 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 582 ctagcagaga cgtggtggtc 20 <210> 583 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 583 gaaggtgacc aagttcatgc taataatttt gaaaacggaa aaagcctact g 51 <210> 584 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 584 gaaggtcgga gtcaacggat taaaataatt ttgaaaacgg aaaaagccta cta 53 <210> 585 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 585 cgcgtattga ttgggacagt ttttgtatt 29 <210> 586 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 586 gaaggtgacc aagttcatgc tggttaggtt tagtgcaatc taggtc 46 <210> 587 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 587 gaaggtcgga gtcaacggat tggttaggtt tagtgcaatc taggtt 46 <210> 588 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 588 tgaaaactcg tattgaaacg cca 23 <210> 589 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 589 gaaggtgacc aagttcatgc tagcttgcgt ggaagttagg t 41 <210> 590 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 590 gaaggtcgga gtcaacggat tagcttgcgt ggaagttagg c 41 <210> 591 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 591 agtctggctt caattcgcct 20 <210> 592 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 592 gaaggtgacc aagttcatgc tgtcatgccc cctagaacgc t 41 <210> 593 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 593 gaaggtcgga gtcaacggat tgtcatgccc cctagaacgc a 41 <210> 594 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 594 ctgaaaattg cccaggtcgc 20 <210> 595 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 595 gaaggtgacc aagttcatgc tacaaccata agtcgagatc ttcg 44 <210> 596 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 596 gaaggtcgga gtcaacggat tacaaccata agtcgagatc ttca 44 <210> 597 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 597 gaagataaca atggcggccg 20 <210> 598 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 598 gaaggtgacc aagttcatgc tgatagttga tgtcaagaat tgattgtcta aaa 53 <210> 599 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 599 gaaggtcgga gtcaacggat tagttgatgt caagaattga ttgtctaaac 50 <210> 600 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 600 caccttatca agttcaatca gtcaagcaa 29 <210> 601 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 601 gaaggtgacc aagttcatgc tgccgattga ttggacctca cc 42 <210> 602 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 602 gaaggtcgga gtcaacggat tctgccgatt gattggacct cact 44 <210> 603 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 603 gatgccatgt gtctttgtcc atttcttat 29 <210> 604 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 604 gaaggtgacc aagttcatgc ttcattttct aagtcagatt cccgc 45 <210> 605 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 605 gaaggtcgga gtcaacggat ttcattttct aagtcagatt cccgt 45 <210> 606 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 606 aagcattggc cgagctactt 20 <210> 607 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 607 gaaggtgacc aagttcatgc tgacatacct acggtatagg ggt 43 <210> 608 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 608 gaaggtcgga gtcaacggat tgacatacct acggtatagg ggc 43 <210> 609 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 609 cggaggacta ttcattgccg t 21 <210> 610 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 610 gaaggtgacc aagttcatgc tgggtggcgt tgaaccaagt 40 <210> 611 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 611 gaaggtcgga gtcaacggat tgggtggcgt tgaaccaagc 40 <210> 612 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 612 gaagacagct gcaccgtgta 20 <210> 613 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 613 gaaggtgacc aagttcatgc taattgatga aacttataag tgcaagtgg 49 <210> 614 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 614 gaaggtcgga gtcaacggat tgtaattgat gaaacttata agtgcaagtg a 51 <210> 615 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 615 cgttgctcta atctccattc acaaatcat 29 <210> 616 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 616 gaaggtgacc aagttcatgc tacatcggaa tcaatcccca ca 42 <210> 617 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 617 gaaggtcgga gtcaacggat tacatcggaa tcaatcccca cg 42 <210> 618 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 618 gttccttact gcccttgcct 20 <210> 619 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 619 gaaggtgacc aagttcatgc tcaccgggaa agataaacgc g 41 <210> 620 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 620 gaaggtcgga gtcaacggat tcaccgggaa agataaacgc a 41 <210> 621 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 621 attcatgggc tcaacttcag c 21 <210> 622 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 622 gaaggtgacc aagttcatgc tgcactgttg gaatcctaca ctca 44 <210> 623 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 623 gaaggtcgga gtcaacggat tgcactgttg gaatcctaca ctct 44 <210> 624 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 624 ttcaccgtca accatggcat 20 <210> 625 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 625 gaaggtgacc aagttcatgc tccattatcc tgacccagct tc 42 <210> 626 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 626 gaaggtcgga gtcaacggat tccattatcc tgacccagct tt 42 <210> 627 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 627 cagtgcggca gttcaattcc 20 <210> 628 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 628 gaaggtgacc aagttcatgc tacacgattg tttagatttg gttaccc 47 <210> 629 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 629 gaaggtcgga gtcaacggat tacacgattg tttagatttg gttacct 47 <210> 630 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 630 tggggtaacg atcatttagt caaatctaaa 30 <210> 631 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 631 gaaggtgacc aagttcatgc tagtcactca caaacatgac gga 43 <210> 632 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 632 gaaggtcgga gtcaacggat tagtcactca caaacatgac ggg 43 <210> 633 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 633 tgatttgcgt gcttctgaag 20 <210> 634 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 634 gaaggtgacc aagttcatgc tcaccattgg gcttggaatc atat 44 <210> 635 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 635 gaaggtcgga gtcaacggat tcaccattgg gcttggaatc atac 44 <210> 636 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 636 acggacaaaa gaaaagcgag c 21 <210> 637 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 637 gaaggtgacc aagttcatgc ttgcaggttt cttgcctcag g 41 <210> 638 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 638 gaaggtcgga gtcaacggat ttgcaggttt cttgcctcag a 41 <210> 639 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 639 tttcagctgc tctctgcctc 20 <210> 640 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 640 gaaggtgacc aagttcatgc tacattattg gtgcctacat ccg 43 <210> 641 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 641 gaaggtcgga gtcaacggat tacattattg gtgcctacat cct 43 <210> 642 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 642 agttatctca cgtgcatgtg gt 22 <210> 643 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 643 gaaggtgacc aagttcatgc tggtccacag cgggtatgat t 41 <210> 644 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 644 gaaggtcgga gtcaacggat tggtccacag cgggtatgat a 41 <210> 645 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 645 tgctgtacca agatagtaga cca 23 <210> 646 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 646 gaaggtgacc aagttcatgc ttcttaaaac acgatcctac agtcac 46 <210> 647 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 647 gaaggtcgga gtcaacggat ttcttaaaac acgatcctac agtcaa 46 <210> 648 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 648 gatctcaatg taacgtaccc tgt 23 <210> 649 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 649 gaaggtgacc aagttcatgc ttggtactgc gtgcatttcc t 41 <210> 650 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 650 gaaggtcgga gtcaacggat ttggtactgc gtgcatttcc c 41 <210> 651 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 651 gaacgagaag gaccgtggtc 20 <210> 652 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 652 gaaggtgacc aagttcatgc ttgtttggaa tacattgggt cgtg 44 <210> 653 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 653 gaaggtcgga gtcaacggat ttgtttggaa tacattgggt cgtc 44 <210> 654 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 654 agcacattcc caaacccgat 20 <210> 655 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 655 gaaggtgacc aagttcatgc tctctggaac ccggtccga 39 <210> 656 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 656 gaaggtcgga gtcaacggat tctctggaac ccggtccgg 39 <210> 657 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 657 agtcaacatc aggcatgtgt c 21 <210> 658 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 658 gaaggtgacc aagttcatgc ttggacctag gtagagcacg g 41 <210> 659 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 659 gaaggtcgga gtcaacggat ttggacctag gtagagcacg a 41 <210> 660 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 660 atgcaggtgg ctaggtgaag 20 <210> 661 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 661 gaaggtgacc aagttcatgc tgtgacctgc ctaatttggg taag 44 <210> 662 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 662 gaaggtcgga gtcaacggat tgtgacctgc ctaatttggg taat 44 <210> 663 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 663 aatattaagc tggccacaaa gc 22 <210> 664 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 664 gaaggtgacc aagttcatgc taacctcttt ttttaaccta tttttggcaa tttt 54 <210> 665 <211> 52 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 665 gaaggtcgga gtcaacggat tcctcttttt ttaacctatt tttggcaatt tc 52 <210> 666 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 666 catgtttgct tcagatgttt gagttcctt 29 <210> 667 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 667 gaaggtgacc aagttcatgc tattgcacgc gtctcctcgc 40 <210> 668 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 668 gaaggtcgga gtcaacggat tattgcacgc gtctcctcgt 40 <210> 669 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 669 tgtgccgtgg actacaaaag 20 <210> 670 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 670 gaaggtgacc aagttcatgc tactcggggt ggccaatttt 40 <210> 671 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 671 gaaggtcgga gtcaacggat tactcggggt ggccaatttg 40 <210> 672 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 672 cgtagaaact tacaggagca tct 23 <210> 673 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 673 gaaggtgacc aagttcatgc tgaaataatg agtccaagct cgtc 44 <210> 674 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 674 gaaggtcgga gtcaacggat tgaaataatg agtccaagct cgtt 44 <210> 675 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 675 atttgcttgg tcggcattgg 20 <210> 676 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 676 gaaggtgacc aagttcatgc tttttgctgc gaatttggca g 41 <210> 677 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 677 gaaggtcgga gtcaacggat tttttgctgc gaatttggca t 41 <210> 678 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 678 aaaccccatc agacaccgtc 20 <210> 679 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 679 gaaggtgacc aagttcatgc taattggttt aaggtccaag ttataaactg 50 <210> 680 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 680 gaaggtcgga gtcaacggat tcaattggtt taaggtccaa gttataaact a 51 <210> 681 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 681 caccgtctca ttagctcgag agaaa 25 <210> 682 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 682 gaaggtgacc aagttcatgc taaaatagaa acaacaccct gctt 44 <210> 683 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 683 gaaggtcgga gtcaacggat taaaatagaa acaacaccct gctc 44 <210> 684 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 684 ttggcgggtt aaacttgtgt 20 <210> 685 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 685 gaaggtgacc aagttcatgc tcggtcgtcg cttgtctctc 40 <210> 686 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 686 gaaggtcgga gtcaacggat tcggtcgtcg cttgtctctt 40 <210> 687 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 687 tgtgggcgac attgaaagtt 20 <210> 688 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 688 gaaggtgacc aagttcatgc taatcttcat atgtaggcta aacgatcc 48 <210> 689 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 689 gaaggtcgga gtcaacggat tataaatctt catatgtagg ctaaacgatc t 51 <210> 690 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 690 gtcgaagaac ctgcatgagt tgagat 26 <210> 691 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 691 gaaggtgacc aagttcatgc ttttgagaag tgcaacgacc g 41 <210> 692 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 692 gaaggtcgga gtcaacggat ttttgagaag tgcaacgacc a 41 <210> 693 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 693 acgatcaaca tcatcgcact 20 <210> 694 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 694 gaaggtgacc aagttcatgc tacgagatca tgcacacagg c 41 <210> 695 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 695 gaaggtcgga gtcaacggat tacgagatca tgcacacagg g 41 <210> 696 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 696 cctgtgtgct tctgggtctg 20 <210> 697 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 697 gaaggtgacc aagttcatgc tgcatattaa tttgtatctt gaatttgctc g 51 <210> 698 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 698 gaaggtcgga gtcaacggat tgcatattaa tttgtatctt gaatttgctc c 51 <210> 699 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 699 tacaaattac agcacgcttg gaggaattt 29 <210> 700 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 700 gaaggtgacc aagttcatgc tggttagcta tagcaactta acgaac 46 <210> 701 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 701 gaaggtcgga gtcaacggat tggttagcta tagcaactta acgaag 46 <210> 702 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 702 agcgtagaaa cattggttct gtc 23 <210> 703 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 703 gaaggtgacc aagttcatgc tgtccaggtt atttgtaagc ctttgg 46 <210> 704 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 704 gaaggtcgga gtcaacggat tgtccaggtt atttgtaagc ctttgc 46 <210> 705 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 705 tgggtattca taaaacttca cgaaaaccta 30 <210> 706 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 706 gaaggtgacc aagttcatgc tcgaaatgtg ggtgaggtca tga 43 <210> 707 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 707 gaaggtcgga gtcaacggat tcgaaatgtg ggtgaggtca tgg 43 <210> 708 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 708 tgtcgccaaa caatctccca 20 <210> 709 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 709 gaaggtgacc aagttcatgc taatagaggc gtggttgcga g 41 <210> 710 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 710 gaaggtcgga gtcaacggat taatagaggc gtggttgcga a 41 <210> 711 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 711 atagactcgg caaccacacc 20 <210> 712 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 712 gaaggtgacc aagttcatgc taccaccacc tcagatattt tcgat 45 <210> 713 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 713 gaaggtcgga gtcaacggat taccaccacc tcagatattt tcgag 45 <210> 714 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 714 agcgacaaat tggaggatac ga 22 <210> 715 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 715 gaaggtgacc aagttcatgc tacattcaaa tagcaaatga ctaacagatt c 51 <210> 716 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 716 gaaggtcgga gtcaacggat taatacattc aaatagcaaa tgactaacag attt 54 <210> 717 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 717 cctgtcattg ttgatgatgt ctttggtaa 29 <210> 718 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 718 gaaggtgacc aagttcatgc ttagtactgg cgacgtggaa c 41 <210> 719 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 719 gaaggtcgga gtcaacggat ttagtactgg cgacgtggaa t 41 <210> 720 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 720 gtcacttgag cctagatctc ca 22 <210> 721 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 721 gaaggtgacc aagttcatgc tgcctagcag atgcagagcg 40 <210> 722 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 722 gaaggtcgga gtcaacggat tgcctagcag atgcagagct 40 <210> 723 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 723 gagcacgtga acattaccag t 21 <210> 724 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 724 gaaggtgacc aagttcatgc tagcgaattg gatggtgata ttaaac 46 <210> 725 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 725 gaaggtcgga gtcaacggat tagcgaattg gatggtgata ttaaaa 46 <210> 726 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 726 ggtagcaagg ttccaaaatg ttg 23 <210> 727 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 727 gaaggtgacc aagttcatgc tcaatgcaat gcactgtggg t 41 <210> 728 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 728 gaaggtcgga gtcaacggat tcaatgcaat gcactgtggg c 41 <210> 729 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 729 tcctgaaaag aaggcagagg a 21 <210> 730 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 730 gaaggtgacc aagttcatgc tggtcaaggt aaaggtcgtg gt 42 <210> 731 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 731 gaaggtcgga gtcaacggat tggtcaaggt aaaggtcgtg ga 42 <210> 732 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 732 ggatgattaa aatgaccagc acg 23 <210> 733 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 733 gaaggtgacc aagttcatgc taggtcgttg gtccatttaa ttaca 45 <210> 734 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 734 gaaggtcgga gtcaacggat taggtcgttg gtccatttaa ttacg 45 <210> 735 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 735 ttgcttcagc gtgagtaggc 20 <210> 736 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 736 gaaggtgacc aagttcatgc ttcatccgtg gaagggtaaa ttc 43 <210> 737 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 737 gaaggtcgga gtcaacggat ttcatccgtg gaagggtaaa ttt 43 <210> 738 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 738 tggcaaaact aactgcgaga 20 <210> 739 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 739 gaaggtgacc aagttcatgc ttgtaccaac aaaaattcca ttcacc 46 <210> 740 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 740 gaaggtcgga gtcaacggat ttgtaccaac aaaaattcca ttcaca 46 <210> 741 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 741 aggttgtacg tgcgatctca a 21 <210> 742 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 742 gaaggtgacc aagttcatgc tcccctacaa atgggtctac aagaag 46 <210> 743 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 743 gaaggtcgga gtcaacggat tcccctacaa atgggtctac aagaaa 46 <210> 744 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 744 gcccttagaa actagtctcg c 21 <210> 745 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 745 gaaggtgacc aagttcatgc taacaatccc acacatgaaa gaaat 45 <210> 746 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 746 gaaggtcgga gtcaacggat taacaatccc acacatgaaa gaaag 45 <210> 747 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 747 tgtctgtttg ctctcaacag t 21 <210> 748 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 748 gaaggtgacc aagttcatgc tgcaagaaaa ttcaagaatc gatcg 45 <210> 749 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 749 gaaggtcgga gtcaacggat tgcaagaaaa ttcaagaatc gatcc 45 <210> 750 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 750 tgtctctcga cttcatttct tagaa 25 <210> 751 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 751 gaaggtgacc aagttcatgc tcctcttcaa cctcttcctt ccc 43 <210> 752 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 752 gaaggtcgga gtcaacggat tcctcttcaa cctcttcctt cct 43 <210> 753 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 753 aattggctat ggtagttaaa ggatg 25 <210> 754 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 754 gaaggtgacc aagttcatgc ttttaattta ggagccaaag tcagtc 46 <210> 755 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 755 gaaggtcgga gtcaacggat ttttaattta ggagccaaag tcagtt 46 <210> 756 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 756 cagcccaaat caccatgaac c 21 <210> 757 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 757 gaaggtgacc aagttcatgc tagctcacct gatctggaat cg 42 <210> 758 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 758 gaaggtcgga gtcaacggat tagctcacct gatctggaat ca 42 <210> 759 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 759 acatttggcg caaataaagg g 21 <210> 760 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 760 gaaggtgacc aagttcatgc tttgatttgc taaatgtagg acaaca 46 <210> 761 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 761 gaaggtcgga gtcaacggat tttgatttgc taaatgtagg acaacg 46 <210> 762 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 762 accatcttca cctccacttg aa 22 <210> 763 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 763 gaaggtgacc aagttcatgc tagtgttttc agaaggtgag gc 42 <210> 764 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 764 gaaggtcgga gtcaacggat tagtgttttc agaaggtgag gt 42 <210> 765 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 765 tggaggtggc aaattttaag tg 22 <210> 766 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 766 gaaggtgacc aagttcatgc ttgatgtgca tccaactcag tg 42 <210> 767 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 767 gaaggtcgga gtcaacggat ttgatgtgca tccaactcag ta 42 <210> 768 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 768 gccaactccc gatccacata 20 <210> 769 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 769 gaaggtgacc aagttcatgc tccacaacag ttcctaaagt tagagc 46 <210> 770 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 770 gaaggtcgga gtcaacggat tccacaacag ttcctaaagt tagagt 46 <210> 771 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 771 aaagttcttc cacgaatcgc c 21 <210> 772 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 772 gaaggtgacc aagttcatgc tagccctacg aaacaagtga gg 42 <210> 773 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 773 gaaggtcgga gtcaacggat tagccctacg aaacaagtga ga 42 <210> 774 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 774 actgacattg aagacccact t 21 <210> 775 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 775 gaaggtgacc aagttcatgc taagagtttc aaagagttgt tcactc 46 <210> 776 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 776 gaaggtcgga gtcaacggat taagagtttc aaagagttgt tcactt 46 <210> 777 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 777 ataggtgtgc tctctccatt ct 22 <210> 778 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 778 gaaggtgacc aagttcatgc tacaacatca aagtgagttg attcag 46 <210> 779 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 779 gaaggtcgga gtcaacggat tacaacatca aagtgagttg attcaa 46 <210> 780 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 780 gacatcgcgt ttgtagtggg 20 <210> 781 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 781 gaaggtgacc aagttcatgc tggaatagaa tgccttagct tcatca 46 <210> 782 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 782 gaaggtcgga gtcaacggat tggaatagaa tgccttagct tcatcg 46 <210> 783 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 783 caagcagatt gttgggcact a 21 <210> 784 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 784 gaaggtgacc aagttcatgc tacaatgcgt actcacccaa aac 43 <210> 785 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 785 gaaggtcgga gtcaacggat tacaatgcgt actcacccaa aat 43 <210> 786 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 786 atggagtttg ggcatatgat tgc 23 <210> 787 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 787 gaaggtgacc aagttcatgc tacactatgg tgggcatgtt cg 42 <210> 788 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 788 gaaggtcgga gtcaacggat tacactatgg tgggcatgtt ca 42 <210> 789 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 789 gtgtgtcaca tccaaagttg g 21 <210> 790 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 790 gaaggtgacc aagttcatgc ttcatcttta tgcggcatga tgt 43 <210> 791 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 791 gaaggtcgga gtcaacggat ttcatcttta tgcggcatga tgg 43 <210> 792 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 792 ggaaaaacgt tctgcaaact gc 22 <210> 793 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 793 gaaggtgacc aagttcatgc tttcaaaagt catgcaatcc ctct 44 <210> 794 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 794 gaaggtcgga gtcaacggat tttcaaaagt catgcaatcc ctcc 44 <210> 795 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 795 ggacgactat ttatacagct agga 24 <210> 796 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 796 gaaggtgacc aagttcatgc taagttccag gtccttcagt tgg 43 <210> 797 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 797 gaaggtcgga gtcaacggat taagttccag gtccttcagt tga 43 <210> 798 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 798 acatgctttc ttatcagacg aga 23 <210> 799 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 799 gaaggtgacc aagttcatgc tctaagttca tattcgttgg ccatag 46 <210> 800 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 800 gaaggtcgga gtcaacggat tctaagttca tattcgttgg ccatac 46 <210> 801 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 801 agaccgataa gtgcaggatc ac 22 <210> 802 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 802 gaaggtgacc aagttcatgc tcccattctc agaaacaaac taacac 46 <210> 803 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 803 gaaggtcgga gtcaacggat tcccattctc agaaacaaac taacat 46 <210> 804 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 804 acgacacatg gtttctccct 20 <210> 805 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 805 gaaggtgacc aagttcatgc tcaaaagagc cacgccccaa g 41 <210> 806 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 806 gaaggtcgga gtcaacggat tcaaaagagc cacgccccaa c 41 <210> 807 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 807 gctcaacaag gccacaagtg 20 <210> 808 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 808 gaaggtgacc aagttcatgc tacatctctg caacaaacaa ttcgg 45 <210> 809 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 809 gaaggtcgga gtcaacggat tacatctctg caacaaacaa ttcga 45 <210> 810 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 810 agtgtcatcg actaatgaac ca 22 <210> 811 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 811 gaaggtgacc aagttcatgc tggggcctaa caaaagactt gttt 44 <210> 812 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 812 gaaggtcgga gtcaacggat tggggcctaa caaaagactt gttg 44 <210> 813 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 813 aaagagctcg gggcaaacaa 20 <210> 814 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 814 gaaggtgacc aagttcatgc tgttgaaatt tctcattcaa gagcga 46 <210> 815 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 815 gaaggtcgga gtcaacggat tgttgaaatt tctcattcaa gagcgg 46 <210> 816 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 816 aaactcattt ttgaaaacat gtcgt 25 <210> 817 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 817 gaaggtgacc aagttcatgc tggagctatt tttggtcgat aacag 45 <210> 818 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 818 gaaggtcgga gtcaacggat tggagctatt tttggtcgat aacaa 45 <210> 819 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 819 agccaaaatt ctgctcgaga 20 <210> 820 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 820 gaaggtgacc aagttcatgc tacacgtgca tcatacatgt atca 44 <210> 821 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 821 gaaggtcgga gtcaacggat tacacgtgca tcatacatgt atcg 44 <210> 822 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 822 gtcatttcgt atgttcgtgt gt 22 <210> 823 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 823 gaaggtgacc aagttcatgc tacatatacc ctgacatcca gct 43 <210> 824 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 824 gaaggtcgga gtcaacggat tacatatacc ctgacatcca gcc 43 <210> 825 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 825 ggccgtgctt tgttatttct t 21 <210> 826 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 826 gaaggtgacc aagttcatgc taaacagatc aggaaacaca gtgg 44 <210> 827 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 827 gaaggtcgga gtcaacggat taaacagatc aggaaacaca gtga 44 <210> 828 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 828 tgatccctct gggcctctag 20 <210> 829 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 829 gaaggtgacc aagttcatgc ttgggaataa aagacaacat cacctg 46 <210> 830 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 830 gaaggtcgga gtcaacggat ttgggaataa aagacaacat caccta 46 <210> 831 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 831 agcaagtcta catctctgcc at 22 <210> 832 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 832 gaaggtgacc aagttcatgc tatggtttca atagttcaat cgaagt 46 <210> 833 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 833 gaaggtcgga gtcaacggat tatggtttca atagttcaat cgaagc 46 <210> 834 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 834 tggtcgttgc aatagaaatt ctc 23 <210> 835 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 835 gaaggtgacc aagttcatgc tacccatctg gagaatttgg tacc 44 <210> 836 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 836 gaaggtcgga gtcaacggat tacccatctg gagaatttgg taca 44 <210> 837 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 837 ctccagaagt aaaatttgtt ccagc 25 <210> 838 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 838 gaaggtgacc aagttcatgc taactaagga ttaagtacta ggcgac 46 <210> 839 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 839 gaaggtcgga gtcaacggat taactaagga ttaagtacta ggcgat 46 <210> 840 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 840 cttgaacctt ggagaccttc a 21 <210> 841 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 841 gaaggtgacc aagttcatgc tcctcgttta tcgccttcct taac 44 <210> 842 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 842 gaaggtcgga gtcaacggat tcctcgttta tcgccttcct taag 44 <210> 843 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 843 acacacacac atggttgttg a 21 <210> 844 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 844 gaaggtgacc aagttcatgc tcttagcttc ctgcatgtaa gaatc 45 <210> 845 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 845 gaaggtcgga gtcaacggat tcttagcttc ctgcatgtaa gaatt 45 <210> 846 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 846 cttggccttc cttcaccgat 20 <210> 847 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 847 gaaggtgacc aagttcatgc tacaattgtc aagcaaagca tcag 44 <210> 848 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 848 gaaggtcgga gtcaacggat tacaattgtc aagcaaagca tcaa 44 <210> 849 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 849 actcagtcca ctagcaactt cc 22 <210> 850 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 850 gaaggtgacc aagttcatgc tgttaaggaa ttaacaatgc gggtag 46 <210> 851 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 851 gaaggtcgga gtcaacggat tgttaaggaa ttaacaatgc gggtaa 46 <210> 852 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 852 ggatgagtct gctgaaggct 20 <210> 853 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 853 gaaggtgacc aagttcatgc tttatatcct aggcccaaat cacac 45 <210> 854 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 854 gaaggtcgga gtcaacggat tttatatcct aggcccaaat cacaa 45 <210> 855 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 855 acatgcattt ccaatgaagc ca 22 <210> 856 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 856 gaaggtgacc aagttcatgc ttgatcaaac catagaagga gagtta 46 <210> 857 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 857 gaaggtcgga gtcaacggat ttgatcaaac catagaagga gagttg 46 <210> 858 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 858 agctatcaca caccccaagc 20 <210> 859 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 859 gaaggtgacc aagttcatgc tccttgtcgt tcattgggac tatatt 46 <210> 860 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 860 gaaggtcgga gtcaacggat tccttgtcgt tcattgggac tatata 46 <210> 861 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 861 tccaagtctt caatctttca gacg 24 <210> 862 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 862 gaaggtgacc aagttcatgc tacaataaaa gccttttgca gcaga 45 <210> 863 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 863 gaaggtcgga gtcaacggat tacaataaaa gccttttgca gcagc 45 <210> 864 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 864 gcatgcttag cgttacagga 20 <210> 865 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 865 gaaggtgacc aagttcatgc tagcaaagga agcagtatac ctaagg 46 <210> 866 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 866 gaaggtcgga gtcaacggat tagcaaagga agcagtatac ctaaga 46 <210> 867 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 867 tgccctactg ttgtcatagt attaa 25 <210> 868 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 868 gaaggtgacc aagttcatgc ttgtcagact gaaggaatca atcatg 46 <210> 869 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 869 gaaggtcgga gtcaacggat ttgtcagact gaaggaatca atcata 46 <210> 870 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 870 ttaaggagtt gtgggagctg c 21 <210> 871 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 871 gaaggtgacc aagttcatgc tcttttctaa tacaggctac ctcacc 46 <210> 872 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 872 gaaggtcgga gtcaacggat tcttttctaa tacaggctac ctcaca 46 <210> 873 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 873 gaacatgatt gagatgtcaa agagt 25 <210> 874 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 874 gaaggtgacc aagttcatgc taccctgcca cggtacaga 39 <210> 875 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 875 gaaggtcgga gtcaacggat taccctgcca cggtacagt 39 <210> 876 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 876 aaaagcattg caccagccg 19 <210> 877 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 877 gaaggtgacc aagttcatgc tgactcgaat caatgatgca tcct 44 <210> 878 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 878 gaaggtcgga gtcaacggat tgactcgaat caatgatgca tccc 44 <210> 879 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 879 aaccaaccga catagagaaa aatt 24 <210> 880 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 880 gaaggtgacc aagttcatgc tctgttcaag accggaccgt c 41 <210> 881 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 881 gaaggtcgga gtcaacggat tctgttcaag accggaccgt a 41 <210> 882 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 882 caaatgcttg gcctcagtcg 20 <210> 883 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 883 gaaggtgacc aagttcatgc tggtttagcg tttagtgaaa gaaga 45 <210> 884 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 884 gaaggtcgga gtcaacggat tggtttagcg tttagtgaaa gaagg 45 <210> 885 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 885 agaaagacca aaatgcacag ga 22 <210> 886 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 886 gaaggtgacc aagttcatgc taaaagggaa gttgttcaaa ctcc 44 <210> 887 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 887 gaaggtcgga gtcaacggat taaaagggaa gttgttcaaa ctca 44 <210> 888 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 888 gaactacacc tattgctcag cg 22 <210> 889 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 889 gaaggtgacc aagttcatgc taccatttag agtgtggggg g 41 <210> 890 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 890 gaaggtcgga gtcaacggat taccatttag agtgtggggg a 41 <210> 891 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 891 tgtgggtttg gatctttgtg tt 22 <210> 892 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 892 gaaggtgacc aagttcatgc tctcatgggt ctcttcaacc tgt 43 <210> 893 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 893 gaaggtcgga gtcaacggat tctcatgggt ctcttcaacc tgg 43 <210> 894 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 894 tcttcccttc tgttgtcagc a 21 <210> 895 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 895 gaaggtgacc aagttcatgc tcacgaggaa caaaattggc attt 44 <210> 896 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 896 gaaggtcgga gtcaacggat tcacgaggaa caaaattggc attc 44 <210> 897 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 897 atcagaaaat tttagcctgg cct 23 <210> 898 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 898 gaaggtgacc aagttcatgc tgtttgtggc atttcaatgt atacgc 46 <210> 899 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 899 gaaggtcgga gtcaacggat tgtttgtggc atttcaatgt atacgt 46 <210> 900 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 900 gtgagcatcg caacactaca a 21 <210> 901 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 901 gaaggtgacc aagttcatgc ttcgaaaaat tgaagagttc cggc 44 <210> 902 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 902 gaaggtcgga gtcaacggat ttcgaaaaat tgaagagttc cggg 44 <210> 903 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 903 tggatagctt cccttctctc gt 22 <210> 904 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 904 gaaggtgacc aagttcatgc ttcctttaga atccaccaat cttctg 46 <210> 905 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 905 gaaggtcgga gtcaacggat ttcctttaga atccaccaat cttcta 46 <210> 906 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 906 accaaaggaa gctgagaaat gg 22 <210> 907 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 907 gaaggtgacc aagttcatgc ttcaaattga agaacacctc cactt 45 <210> 908 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 908 gaaggtcgga gtcaacggat ttcaaattga agaacacctc cactg 45 <210> 909 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 909 tcccaatcca cttaaggtta cca 23 <210> 910 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 910 gaaggtgacc aagttcatgc tagccatgaa gttctttttg agtgg 45 <210> 911 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 911 gaaggtcgga gtcaacggat tagccatgaa gttctttttg agtgt 45 <210> 912 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 912 ccaaggcatt aacacatcgc aa 22 <210> 913 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 913 gaaggtgacc aagttcatgc tgaatgctca gaggatagga aacg 44 <210> 914 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 914 gaaggtcgga gtcaacggat tgaatgctca gaggatagga aaca 44 <210> 915 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 915 tgaaccagga aatcaggttg t 21 <210> 916 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 916 gaaggtgacc aagttcatgc tacgattctt tgatttatag gagggc 46 <210> 917 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 917 gaaggtcgga gtcaacggat tacgattctt tgatttatag gagggt 46 <210> 918 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 918 agttttccga ctttatggag aaacc 25 <210> 919 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 919 gaaggtgacc aagttcatgc tgagttgcat tggcttacac taaatc 46 <210> 920 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 920 gaaggtcgga gtcaacggat tgagttgcat tggcttacac taaatt 46 <210> 921 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 921 atttgacttt gtttttaaac cacgt 25 <210> 922 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 922 gaaggtgacc aagttcatgc ttgctaaaat aagtgagtgg cttgt 45 <210> 923 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 923 gaaggtcgga gtcaacggat ttgctaaaat aagtgagtgg cttgc 45 <210> 924 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 924 actagaaaaa catgccctcg a 21 <210> 925 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 925 gaaggtgacc aagttcatgc tagcttgccg gtgtgggtt 39 <210> 926 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 926 gaaggtcgga gtcaacggat tagcttgccg gtgtgggtc 39 <210> 927 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 927 tgttgttcat tcggtgcttt gc 22 <210> 928 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 928 gaaggtgacc aagttcatgc tctggagccc aataatttga gtga 44 <210> 929 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 929 gaaggtcgga gtcaacggat tctggagccc aataatttga gtgg 44 <210> 930 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 930 acttgggact cttgacagca 20 <210> 931 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 931 gaaggtgacc aagttcatgc tacgttaagc tctatctctt gttct 45 <210> 932 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 932 gaaggtcgga gtcaacggat tacgttaagc tctatctctt gttcc 45 <210> 933 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 933 catccttagc acccttgtcc t 21 <210> 934 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 934 gaaggtgacc aagttcatgc tttggttgct cgacataatt gttt 44 <210> 935 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 935 gaaggtcgga gtcaacggat tttggttgct cgacataatt gttc 44 <210> 936 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 936 tgcaaaacct aatctccggc t 21 <210> 937 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 937 gaaggtgacc aagttcatgc tcggactatc atggcttgtt gtt 43 <210> 938 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 938 gaaggtcgga gtcaacggat tcggactatc atggcttgtt gtc 43 <210> 939 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 939 cgagtcacca tcgacttcct 20 <210> 940 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 940 gaaggtgacc aagttcatgc ttgcctcatg gactgaagtt tttg 44 <210> 941 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 941 gaaggtcgga gtcaacggat ttgcctcatg gactgaagtt ttta 44 <210> 942 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 942 gtttcaacca ctaccaatcg ct 22 <210> 943 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 943 gaaggtgacc aagttcatgc tcgacgaact tcggttacaa agac 44 <210> 944 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 944 gaaggtcgga gtcaacggat tcgacgaact tcggttacaa agat 44 <210> 945 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 945 atgttgttga gacatgcgcc 20 <210> 946 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 946 gaaggtgacc aagttcatgc tgggggaact gtggtgcaac 40 <210> 947 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 947 gaaggtcgga gtcaacggat tgggggaact gtggtgcaat 40 <210> 948 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 948 tttccagtgg tctctgacag c 21 <210> 949 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 949 gaaggtgacc aagttcatgc tggctcatgg ataaaagtcg aacag 45 <210> 950 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 950 gaaggtcgga gtcaacggat tggctcatgg ataaaagtcg aacaa 45 <210> 951 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 951 ggtgtgcctg attttgccat 20 <210> 952 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 952 gaaggtgacc aagttcatgc tcctcccgat gcataaaact gtt 43 <210> 953 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 953 gaaggtcgga gtcaacggat tcctcccgat gcataaaact gtc 43 <210> 954 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 954 ggcgatttgt gcgtcaagag 20 <210> 955 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 955 gaaggtgacc aagttcatgc tcggtctctc tcttaactct cttgtc 46 <210> 956 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 956 gaaggtcgga gtcaacggat tcggtctctc tcttaactct cttgtt 46 <210> 957 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 957 atcttcgtta tgcgtcgaaa gt 22 <210> 958 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 958 gaaggtgacc aagttcatgc taaggatagg aataagccct caag 44 <210> 959 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 959 gaaggtcgga gtcaacggat taaggatagg aataagccct caaa 44 <210> 960 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 960 ttgacgcatc aagattccat aaaat 25 <110> Korea Research Institute of Bioscience and Biotechnology <120> Single nucleotide polymorphism marker set for marker-assisted backcross breeding in Cucumis melo var. makuwa and thereof <130> PN19354 <160> 960 <170> KoPatentIn 3.0 <210> 1 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 1 gaaggtgacc aagttcatgc tgccactgac cttcttcaga act 43 <210> 2 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 2 gaaggtcgga gtcaacggat tgccactgac cttcttcaga acg 43 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 ttcaggtcgc actgctcaat 20 <210> 4 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 gaaggtgacc aagttcatgc ttgtaagatt gtagaacagc ccg 43 <210> 5 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 gaaggtcgga gtcaacggat ttgtaagatt gtagaacagc cca 43 <210> 6 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 gtgcacagat atgttgacca cc 22 <210> 7 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 gaaggtgacc aagttcatgc tgacccgagt tggtgaagct ag 42 <210> 8 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 gaaggtcgga gtcaacggat tgacccgagt tggtgaagct aa 42 <210> 9 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 actgacatgt tccatagggg t 21 <210> 10 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 gaaggtgacc aagttcatgc ttgaatttca attcgagtag gctctt 46 <210> 11 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 gaaggtcgga gtcaacggat ttgaatttca attcgagtag gctctc 46 <210> 12 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 agatgagatg ttcaaggtag ctga 24 <210> 13 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 gaaggtgacc aagttcatgc ttcttttagg tttcgctaaa ctcgtc 46 <210> 14 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 gaaggtcgga gtcaacggat ttcttttagg tttcgctaaa ctcgtt 46 <210> 15 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 aagaagtcta ggagagagag ct 22 <210> 16 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 16 gaaggtgacc aagttcatgc tacacaacta cagactcgat gc 42 <210> 17 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 gaaggtcgga gtcaacggat tacacaacta cagactcgat gg 42 <210> 18 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 18 tcttgggata caaacgtgtt gtt 23 <210> 19 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 19 gaaggtgacc aagttcatgc tctccggacc gccattcag 39 <210> 20 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 20 gaaggtcgga gtcaacggat tctccggacc gccattcaa 39 <210> 21 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 21 tgatggacgc atgtgaagc 19 <210> 22 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 22 gaaggtgacc aagttcatgc tcaccatcta cctctccgat tcattc 46 <210> 23 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 23 gaaggtcgga gtcaacggat tcaccatcta cctctccgat tcatta 46 <210> 24 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 24 gacttggcaa cggaaaaggc 20 <210> 25 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 25 gaaggtgacc aagttcatgc tcctaccagc atctgaaaag gttc 44 <210> 26 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 26 gaaggtcgga gtcaacggat tcctaccagc atctgaaaag gttg 44 <210> 27 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 27 gacatgttcc tcctccagcg 20 <210> 28 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 28 gaaggtgacc aagttcatgc ttttgtacca aactcctcac tagac 45 <210> 29 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 29 gaaggtcgga gtcaacggat ttttgtacca aactcctcac tagat 45 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 30 tgtgggcttg acacactctt 20 <210> 31 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 31 gaaggtgacc aagttcatgc tagtatcgtt cggcagcatc g 41 <210> 32 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 32 gaaggtcgga gtcaacggat tagtatcgtt cggcagcatc a 41 <210> 33 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 33 attccgactt tgcaatccgc 20 <210> 34 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 34 gaaggtgacc aagttcatgc tcctttcatt ttctatggtc tttgacttc 49 <210> 35 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 35 gaaggtcgga gtcaacggat tatcctttca ttttctatgg tctttgactt t 51 <210> 36 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 36 taatgtgatg tcgacacaca cattccaat 29 <210> 37 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 37 gaaggtgacc aagttcatgc tgctgtctga agagtgccaa t 41 <210> 38 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 38 gaaggtcgga gtcaacggat tgctgtctga agagtgccaa c 41 <210> 39 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 39 tctgtcacta gttatgtcag cgt 23 <210> 40 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 40 gaaggtgacc aagttcatgc tgggtcaatc tcggtgctga t 41 <210> 41 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 41 gaaggtcgga gtcaacggat tgggtcaatc tcggtgctga c 41 <210> 42 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 42 tgataggatt ggccagaacg t 21 <210> 43 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 43 gaaggtgacc aagttcatgc tactgaagtg ttatgagtgg ccg 43 <210> 44 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 44 gaaggtcgga gtcaacggat tactgaagtg ttatgagtgg cca 43 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 45 caacaaggag atgcggtggt 20 <210> 46 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 46 gaaggtgacc aagttcatgc tacagatcta agccattcat gatca 45 <210> 47 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 47 gaaggtcgga gtcaacggat tacagatcta agccattcat gatcg 45 <210> 48 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 48 tggagattca tgatccagcg a 21 <210> 49 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 49 gaaggtgacc aagttcatgc tcgatggcgt cgtccttct 39 <210> 50 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 50 gaaggtcgga gtcaacggat tcgatggcgt cgtccttcc 39 <210> 51 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 51 acacatacct tactgatgcc c 21 <210> 52 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 52 gaaggtgacc aagttcatgc tctactacgc tagccaagat actc 44 <210> 53 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 53 gaaggtcgga gtcaacggat tgtctactac gctagccaag atactt 46 <210> 54 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 54 gccagtaagc tactaaacca gggta 25 <210> 55 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 55 gaaggtgacc aagttcatgc tttgacatat cgaagtcgcg ta 42 <210> 56 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 56 gaaggtcgga gtcaacggat tttgacatat cgaagtcgcg tg 42 <210> 57 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 57 agcacgcaat gtatagtcct c 21 <210> 58 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 58 gaaggtgacc aagttcatgc tttgtggtag tgttacgagt tct 43 <210> 59 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 59 gaaggtcgga gtcaacggat tttgtggtag tgttacgagt tcc 43 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 60 tggccagttc agatgatgct 20 <210> 61 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 61 gaaggtgacc aagttcatgc taataagaaa tgggagtgag agtaagg 47 <210> 62 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 62 gaaggtcgga gtcaacggat tcaataagaa atgggagtga gagtaaga 48 <210> 63 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 63 cttcctccat ggtttatttt cgtcacatt 29 <210> 64 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 64 gaaggtgacc aagttcatgc tcgcacagat tcaaaactcg acact 45 <210> 65 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 65 gaaggtcgga gtcaacggat tgcacagatt caaaactcga cacc 44 <210> 66 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 66 atgaaaatat ctagtagcga ccgaacaaaa 30 <210> 67 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 67 gaaggtgacc aagttcatgc tagcagctca gaaccatcac g 41 <210> 68 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 68 gaaggtcgga gtcaacggat tagcagctca gaaccatcac a 41 <210> 69 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 69 aggccggttt aaatggttca 20 <210> 70 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 70 gaaggtgacc aagttcatgc taccttatct ttgagtgtac cctctt 46 <210> 71 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 71 gaaggtcgga gtcaacggat taccttatct ttgagtgtac cctctc 46 <210> 72 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 72 tcttcctcat aaacaacaag tggt 24 <210> 73 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 73 gaaggtgacc aagttcatgc tactaagagg ggtggctaag tca 43 <210> 74 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 74 gaaggtcgga gtcaacggat tactaagagg ggtggctaag tcg 43 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 75 ggattttgag cccttgccca 20 <210> 76 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 76 gaaggtgacc aagttcatgc tatgcctcac cccgcacaac 40 <210> 77 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 77 gaaggtcgga gtcaacggat tatgcctcac cccgcacaat 40 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 78 agttgcacca cgtctcacat 20 <210> 79 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 79 gaaggtgacc aagttcatgc tagactatca gttaacaggc attctt 46 <210> 80 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 80 gaaggtcgga gtcaacggat tagactatca gttaacaggc attcta 46 <210> 81 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 81 tcgttcttgc ggtcttctcc 20 <210> 82 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 82 gaaggtgacc aagttcatgc tatgccgtga ctgcaaagta a 41 <210> 83 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 83 gaaggtcgga gtcaacggat tatgccgtga ctgcaaagta g 41 <210> 84 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 84 gtgctatgaa gacacaccta tca 23 <210> 85 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 85 gaaggtgacc aagttcatgc tcggacggtc atgtggaatc c 41 <210> 86 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 86 gaaggtcgga gtcaacggat tctcggacgg tcatgtggaa tca 43 <210> 87 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 87 gcctcgagat tacacttttg tgttcaat 28 <210> 88 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 88 gaaggtgacc aagttcatgc tgcccaaccc agccgtctg 39 <210> 89 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 89 gaaggtcgga gtcaacggat tgcccaaccc agccgtcta 39 <210> 90 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 90 gtgcacgtag ctggaagga 19 <210> 91 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 91 gaaggtgacc aagttcatgc tagtgctgag aaggctatga aa 42 <210> 92 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 92 gaaggtcgga gtcaacggat tagtgctgag aaggctatga ag 42 <210> 93 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 93 tcaactatca agtaagcatc ggc 23 <210> 94 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 94 gaaggtgacc aagttcatgc tgctgatggt ggttcgaaag tga 43 <210> 95 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 95 gaaggtcgga gtcaacggat tgctgatggt ggttcgaaag tgc 43 <210> 96 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 96 tcgacccaat caaatgcagc 20 <210> 97 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 97 gaaggtgacc aagttcatgc taatactccc gtaaccaccc c 41 <210> 98 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 98 gaaggtcgga gtcaacggat taatactccc gtaaccaccc t 41 <210> 99 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 99 cgaaactttc gcttcacgga 20 <210> 100 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 100 gaaggtgacc aagttcatgc tcatgtgtcc catgtcctat tgaa 44 <210> 101 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 101 gaaggtcgga gtcaacggat tcatgtgtcc catgtcctat tgag 44 <210> 102 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 102 ggccattctt aggggagaac a 21 <210> 103 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 103 gaaggtgacc aagttcatgc tcgtgtgggt taggacaaaa gca 43 <210> 104 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 104 gaaggtcgga gtcaacggat tcgtgtgggt taggacaaaa gct 43 <210> 105 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 105 agaagcttta ggtcgaaaag ga 22 <210> 106 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 106 gaaggtgacc aagttcatgc ttcataagta ctgagttaag ggtgaa 46 <210> 107 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 107 gaaggtcgga gtcaacggat ttcataagta ctgagttaag ggtgat 46 <210> 108 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 108 ggttctgcag taagctttgg c 21 <210> 109 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 109 gaaggtgacc aagttcatgc tagtccccta aagctcgtgt a 41 <210> 110 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 110 gaaggtcgga gtcaacggat tagtccccta aagctcgtgt g 41 <210> 111 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 111 tccccctttc aatttccggg 20 <210> 112 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 112 gaaggtgacc aagttcatgc tgttcaaatt cggttcctac aacttattaa a 51 <210> 113 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 113 gaaggtcgga gtcaacggat tcaaattcgg ttcctacaac ttattaag 48 <210> 114 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 114 ggatgtcgtt aaggtaattg gtcatgttt 29 <210> 115 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 115 gaaggtgacc aagttcatgc tgttctttag gggttcctac tgtcat 46 <210> 116 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 116 gaaggtcgga gtcaacggat tgttctttag gggttcctac tgtcac 46 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 117 ttcgtctcgg agcatagcca 20 <210> 118 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 118 gaaggtgacc aagttcatgc tgcagacctc cttagtattc tcact 45 <210> 119 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 119 gaaggtcgga gtcaacggat tgcagacctc cttagtattc tcacc 45 <210> 120 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 120 gaactggttc tgaaggatgt ca 22 <210> 121 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 121 gaaggtgacc aagttcatgc ttgccggcca tctttaaaga ctg 43 <210> 122 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 122 gaaggtcgga gtcaacggat ttgccggcca tctttaaaga cta 43 <210> 123 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 123 tggacctgtt tgtcgagagt g 21 <210> 124 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 124 gaaggtgacc aagttcatgc ttctcagccg tttgatccaa ta 42 <210> 125 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 125 gaaggtcgga gtcaacggat ttctcagccg tttgatccaa tg 42 <210> 126 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 126 cgtggcagag atcctctacg 20 <210> 127 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 127 gaaggtgacc aagttcatgc tcctccccat gcctttgtgt 40 <210> 128 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 128 gaaggtcgga gtcaacggat tcctccccat gcctttgtgg 40 <210> 129 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 129 tcggtattgt ccagcaagca 20 <210> 130 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 130 gaaggtgacc aagttcatgc taaagttagg cttgccttca tcatgc 46 <210> 131 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 131 gaaggtcgga gtcaacggat taaagttagg cttgccttca tcatgt 46 <210> 132 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 132 ctcgaaccca ctcgagagga cta 23 <210> 133 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 133 gaaggtgacc aagttcatgc tctgtcatcc cgtctcaatg ag 42 <210> 134 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 134 gaaggtcgga gtcaacggat tctgtcatcc cgtctcaatg aa 42 <210> 135 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 135 ctgcctacgc cctaccaaaa 20 <210> 136 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 136 gaaggtgacc aagttcatgc taggaaagga aagttaaagt cgcg 44 <210> 137 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 137 gaaggtcgga gtcaacggat taggaaagga aagttaaagt cgcc 44 <210> 138 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 138 tgattcctgt ctgattgttg ct 22 <210> 139 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 139 gaaggtgacc aagttcatgc tagtattata tgtagataat attctactca ttgg 54 <210> 140 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 140 gaaggtcgga gtcaacggat tagtattata tgtagataat attctactca ttgt 54 <210> 141 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 141 attgatatca gtaagataac gtacgacatt 30 <210> 142 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 142 gaaggtgacc aagttcatgc tagtgcattt acgggacttc a 41 <210> 143 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 143 gaaggtcgga gtcaacggat tagtgcattt acgggacttc g 41 <210> 144 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 gctccaacga tcccagagt 19 <210> 145 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 gaaggtgacc aagttcatgc tgagcacatg tcaaagcagt agtatct 47 <210> 146 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 146 gaaggtcgga gtcaacggat tagcacatgt caaagcagta gtatcc 46 <210> 147 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 cgtttaagag tctttgttgg cttagagat 29 <210> 148 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 gaaggtgacc aagttcatgc tacacaaccg aattcgagaa aag 43 <210> 149 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 149 gaaggtcgga gtcaacggat tacacaaccg aattcgagaa aaa 43 <210> 150 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 tggtaggccc gtttgtttgt 20 <210> 151 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 gaaggtgacc aagttcatgc tagtgtcgcg ccaacttatg g 41 <210> 152 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 152 gaaggtcgga gtcaacggat tagtgtcgcg ccaacttatg a 41 <210> 153 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 tcactgatgt attaacacgg ct 22 <210> 154 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 gaaggtgacc aagttcatgc tgattgaggt ctagaggctg act 43 <210> 155 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 155 gaaggtcgga gtcaacggat tgattgaggt ctagaggctg acc 43 <210> 156 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 cacccaataa gtgcggaaac a 21 <210> 157 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 gaaggtgacc aagttcatgc taggtaatca gagattgcat ccag 44 <210> 158 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 158 gaaggtcgga gtcaacggat taggtaatca gagattgcat ccat 44 <210> 159 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 ttccaacaca ggcatcacga 20 <210> 160 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 gaaggtgacc aagttcatgc tgtcttatgg tgatgttcta ggtagt 46 <210> 161 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 161 gaaggtcgga gtcaacggat tgtcttatgg tgatgttcta ggtagc 46 <210> 162 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 tcacctagtc acttgtttga ggt 23 <210> 163 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 gaaggtgacc aagttcatgc ttcttgatcc tccttagcgt gaa 43 <210> 164 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 164 gaaggtcgga gtcaacggat ttcttgatcc tccttagcgt gat 43 <210> 165 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 tcctcctcat cacgatgaag a 21 <210> 166 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 gaaggtgacc aagttcatgc tagttcgtta agtcgggcaa aac 43 <210> 167 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 167 gaaggtcgga gtcaacggat tagttcgtta agtcgggcaa aat 43 <210> 168 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 tggccaataa gtcgtcatgc 20 <210> 169 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 gaaggtgacc aagttcatgc tactgcacat aaactgttat gggtatag 48 <210> 170 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 170 gaaggtcgga gtcaacggat tataactgca cataaactgt tatgggtata t 51 <210> 171 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 aatagggaag tagggtggtc ttaacattt 29 <210> 172 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 gaaggtgacc aagttcatgc tagtccacga cagagcaaat t 41 <210> 173 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 173 gaaggtcgga gtcaacggat tagtccacga cagagcaaat c 41 <210> 174 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 actgggtctt cgtcggagat 20 <210> 175 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 gaaggtgacc aagttcatgc tggattttgt gtacatgaac tgaggg 46 <210> 176 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 176 gaaggtcgga gtcaacggat tggattttgt gtacatgaac tgagga 46 <210> 177 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 gtgagaaaca gtcaagttgg ca 22 <210> 178 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 gaaggtgacc aagttcatgc ttggtttaac atctgtgtgg aaaca 45 <210> 179 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 179 gaaggtcgga gtcaacggat ttggtttaac atctgtgtgg aaacg 45 <210> 180 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 gggaggttgg agaacgttgt 20 <210> 181 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 gaaggtgacc aagttcatgc tatgaaatca accctctcgg ag 42 <210> 182 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 182 gaaggtcgga gtcaacggat tatgaaatca accctctcgg ac 42 <210> 183 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 tgtgtccaca tgtcatagct ga 22 <210> 184 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 gaaggtgacc aagttcatgc tggaaaaaca tatggcatgc acg 43 <210> 185 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 185 gaaggtcgga gtcaacggat tggaaaaaca tatggcatgc act 43 <210> 186 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 gctgtacctg cagaatgatg a 21 <210> 187 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 gaaggtgacc aagttcatgc tgttattgct gatcgtaagc atcaaac 47 <210> 188 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 188 gaaggtcgga gtcaacggat tatgttattg ctgatcgtaa gcatcaaat 49 <210> 189 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 ttaaagacat gagtccaaca tcccatgaa 29 <210> 190 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 gaaggtgacc aagttcatgc tgctgtcgtt gatgttccca at 42 <210> 191 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 191 gaaggtcgga gtcaacggat tgctgtcgtt gatgttccca ac 42 <210> 192 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 tgctgtgctg gttccatgtt 20 <210> 193 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 gaaggtgacc aagttcatgc tggattatgt tcggttgaag gcg 43 <210> 194 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 194 gaaggtcgga gtcaacggat tggattatgt tcggttgaag gcc 43 <210> 195 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 gtcggatcat tgaaagggca t 21 <210> 196 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 gaaggtgacc aagttcatgc tgcgagtaga ctatcacgca gtc 43 <210> 197 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 197 gaaggtcgga gtcaacggat tgcgagtaga ctatcacgca gtt 43 <210> 198 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 atgcgccacc tgtctttgta 20 <210> 199 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 gaaggtgacc aagttcatgc tagcattagg agtggtgtgt aataat 46 <210> 200 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 200 gaaggtcgga gtcaacggat tagcattagg agtggtgtgt aataac 46 <210> 201 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 cccagtgtca catgtccctc 20 <210> 202 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 gaaggtgacc aagttcatgc tcaaaagata caaagctcta cctcgg 46 <210> 203 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 203 gaaggtcgga gtcaacggat tcaaaagata caaagctcta cctcga 46 <210> 204 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 204 tggttccttg ctgaagaggt 20 <210> 205 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 205 gaaggtgacc aagttcatgc tgcagctcgt aaccggaaat tc 42 <210> 206 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 206 gaaggtcgga gtcaacggat tgcagctcgt aaccggaaat tt 42 <210> 207 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 207 ctctttcacc ggagagctca a 21 <210> 208 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 208 gaaggtgacc aagttcatgc ttcaaagttc ccattgctca caag 44 <210> 209 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 209 gaaggtcgga gtcaacggat ttcaaagttc ccattgctca caaa 44 <210> 210 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 210 acctgtggta ttttgccgtg a 21 <210> 211 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 211 gaaggtgacc aagttcatgc ttcattctat acactggtta gccac 45 <210> 212 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 212 gaaggtcgga gtcaacggat ttcattctat acactggtta gccaa 45 <210> 213 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 213 aaagaagtgg ccccctttgg 20 <210> 214 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 214 gaaggtgacc aagttcatgc tgaatgtcat aagtcgtatt cacacg 46 <210> 215 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 215 gaaggtcgga gtcaacggat tgaatgtcat aagtcgtatt cacaca 46 <210> 216 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 216 gcttgcaaaa ctagacaaca cg 22 <210> 217 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 217 gaaggtgacc aagttcatgc tggttctaag gatgctgtgg gttc 44 <210> 218 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 218 gaaggtcgga gtcaacggat tggttctaag gatgctgtgg gtta 44 <210> 219 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 219 aaaatgggtc aaagccttgc tc 22 <210> 220 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 220 gaaggtgacc aagttcatgc tgaagaaacg ttgcacccaa taa 43 <210> 221 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 221 gaaggtcgga gtcaacggat tgaagaaacg ttgcacccaa tag 43 <210> 222 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 222 aggtggaatc cagatgctat tgt 23 <210> 223 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 223 gaaggtgacc aagttcatgc tccccgatta taacaggttg cacc 44 <210> 224 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 224 gaaggtcgga gtcaacggat taccccgatt ataacaggtt gcaca 45 <210> 225 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 225 gtcggtggtt attatagttt gtcaggtat 29 <210> 226 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 gaaggtgacc aagttcatgc tagaaacctg atgcagactg at 42 <210> 227 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 227 gaaggtcgga gtcaacggat tagaaacctg atgcagactg ac 42 <210> 228 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 agttgcaact cctggtcgtt 20 <210> 229 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 gaaggtgacc aagttcatgc tgtagtcata attgcgctgt taacg 45 <210> 230 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 230 gaaggtcgga gtcaacggat tgtagtcata attgcgctgt taaca 45 <210> 231 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 231 aaagagagtt agacttccat cgt 23 <210> 232 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 232 gaaggtgacc aagttcatgc tgaaatctaa acaagaaaga ttcatatcct c 51 <210> 233 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 233 gaaggtcgga gtcaacggat tctgaaatct aaacaagaaa gattcatatc ctt 53 <210> 234 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 234 ccaccttcat ctatggtgct tgcta 25 <210> 235 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 235 gaaggtgacc aagttcatgc tgtgtttagc ataatcgtga ccgc 44 <210> 236 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 236 gaaggtcgga gtcaacggat tgtgtttagc ataatcgtga ccgt 44 <210> 237 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 237 aggcttgtgt gactctcgc 19 <210> 238 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 238 gaaggtgacc aagttcatgc tgaacaaaac tccaccggtg g 41 <210> 239 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 239 gaaggtcgga gtcaacggat tgaacaaaac tccaccggtg t 41 <210> 240 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 240 gacgccggag ataagggtag 20 <210> 241 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 241 gaaggtgacc aagttcatgc tcaatcttca ataacgcggc ct 42 <210> 242 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 242 gaaggtcgga gtcaacggat tcaatcttca ataacgcggc cg 42 <210> 243 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 243 ttcctctact tcctccgcga 20 <210> 244 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 244 gaaggtgacc aagttcatgc tggacacatg cctcattagc ga 42 <210> 245 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 245 gaaggtcgga gtcaacggat tggacacatg cctcattagc gg 42 <210> 246 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 246 gcgtacgagc tagaattcct 20 <210> 247 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 247 gaaggtgacc aagttcatgc ttttccggtg cccctcctta a 41 <210> 248 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 248 gaaggtcgga gtcaacggat ttttccggtg cccctcctta c 41 <210> 249 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 249 agctcaactc gagagggagg 20 <210> 250 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 250 gaaggtgacc aagttcatgc tgccaatcta gtgagcatgg ga 42 <210> 251 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 251 gaaggtcgga gtcaacggat tgccaatcta gtgagcatgg gg 42 <210> 252 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 252 gcaaattctg cggttcgtgc 20 <210> 253 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 253 gaaggtgacc aagttcatgc tctgtctgga caattatgcg ca 42 <210> 254 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 254 gaaggtcgga gtcaacggat tctgtctgga caattatgcg ct 42 <210> 255 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 255 tggatcccat gaggtaccca 20 <210> 256 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 256 gaaggtgacc aagttcatgc ttctacaggc ttcggcaatc a 41 <210> 257 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 257 gaaggtcgga gtcaacggat ttctacaggc ttcggcaatc g 41 <210> 258 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 258 gcccctactt gaagttcggt 20 <210> 259 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 259 gaaggtgacc aagttcatgc tgaatcaact tgttcaaaac cgacacg 47 <210> 260 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 260 gaaggtcgga gtcaacggat tgaatcaact tgttcaaaac cgacaca 47 <210> 261 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 261 gggaaagttc agctcaaaag catcataa 28 <210> 262 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 262 gaaggtgacc aagttcatgc tgcctacttc tgaacaactt gcg 43 <210> 263 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 263 gaaggtcgga gtcaacggat tgcctacttc tgaacaactt gca 43 <210> 264 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 264 tgcatacttc acgctcagct 20 <210> 265 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 265 gaaggtgacc aagttcatgc tttttcgaag gaggccgcgg 40 <210> 266 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 266 gaaggtcgga gtcaacggat tttttcgaag gaggccgcga 40 <210> 267 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 267 acgcccgtaa gagttcactc 20 <210> 268 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 268 gaaggtgacc aagttcatgc tccgtcgcct ccccctacc 39 <210> 269 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 269 gaaggtcgga gtcaacggat tccgtcgcct ccccctact 39 <210> 270 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 270 tcaaaacgag gcaaggacgt 20 <210> 271 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 271 gaaggtgacc aagttcatgc tgtaatccag attatcctct cccaaa 46 <210> 272 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 272 gaaggtcgga gtcaacggat tgtaatccag attatcctct cccaag 46 <210> 273 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 273 aggctgttaa acattaaggg gt 22 <210> 274 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 274 gaaggtgacc aagttcatgc tcttcaactt tttgggggca ct 42 <210> 275 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 275 gaaggtcgga gtcaacggat tcttcaactt tttgggggca ca 42 <210> 276 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 276 tatgaacggc gggagtttcc 20 <210> 277 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 277 gaaggtgacc aagttcatgc tatggccatg ataaaagacc atcagg 46 <210> 278 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 278 gaaggtcgga gtcaacggat tgatggccat gataaaagac catcaga 47 <210> 279 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 279 gtcatgattg tccagataac cacgtttt 28 <210> 280 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 280 gaaggtgacc aagttcatgc tacacgattt tcctctctag gcc 43 <210> 281 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 281 gaaggtcgga gtcaacggat tacacgattt tcctctctag gct 43 <210> 282 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 282 cgcgtaccaa aggaaatcgc 20 <210> 283 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 283 gaaggtgacc aagttcatgc tgaatcaagc taagcatggt ctgaac 46 <210> 284 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 284 gaaggtcgga gtcaacggat taagaatcaa gctaagcatg gtctgaaa 48 <210> 285 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 285 cggctcctat ttgacacttt atacatgta 29 <210> 286 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 286 gaaggtgacc aagttcatgc tgggaagtgg gattagggat ca 42 <210> 287 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 287 gaaggtcgga gtcaacggat tgggaagtgg gattagggat cg 42 <210> 288 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 288 tcaggccatc tgatctacat gat 23 <210> 289 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 289 gaaggtgacc aagttcatgc tccttctgaa tcaaatgctt agacct 46 <210> 290 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 290 gaaggtcgga gtcaacggat tccttctgaa tcaaatgctt agaccc 46 <210> 291 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 291 tttagttgac atcatgcccc c 21 <210> 292 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 292 gaaggtgacc aagttcatgc taggcttgac gtggtggatt t 41 <210> 293 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 293 gaaggtcgga gtcaacggat taggcttgac gtggtggatt c 41 <210> 294 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 294 tgtgtagtgt tcttccgcgt 20 <210> 295 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 295 gaaggtgacc aagttcatgc ttgaggagtt tccaaaatca acgg 44 <210> 296 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 296 gaaggtcgga gtcaacggat ttgaggagtt tccaaaatca acgc 44 <210> 297 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 297 cagcttccaa agagaggacg a 21 <210> 298 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 298 gaaggtgacc aagttcatgc tcaagggtat tgtccagttt cttagc 46 <210> 299 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 299 gaaggtcgga gtcaacggat tcaagggtat tgtccagttt cttagt 46 <210> 300 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 300 agtacgaaga attgggggcc 20 <210> 301 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 301 gaaggtgacc aagttcatgc tacttgatga gcccaacact tt 42 <210> 302 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 302 gaaggtcgga gtcaacggat tacttgatga gcccaacact tc 42 <210> 303 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 303 cgtgttagtt tgagaggaca ca 22 <210> 304 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 304 gaaggtgacc aagttcatgc ttgacttcga tacaaatggc agacta 46 <210> 305 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 305 gaaggtcgga gtcaacggat ttgacttcga tacaaatggc agactg 46 <210> 306 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 306 acatgacctc gccgctatac 20 <210> 307 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 307 gaaggtgacc aagttcatgc tgccacgctt gttggagatg ac 42 <210> 308 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 308 gaaggtcgga gtcaacggat tggccacgct tgttggagat gat 43 <210> 309 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 309 tctccacttc ctccgaaaat aactagttt 29 <210> 310 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 310 gaaggtgacc aagttcatgc tggaggtgac ggtgatcgcg 40 <210> 311 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 311 gaaggtcgga gtcaacggat tggaggtgac ggtgatcgca 40 <210> 312 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 312 gcgccttcgt ctctcctatc 20 <210> 313 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 313 gaaggtgacc aagttcatgc tgatcgatac acagatgtac atggt 45 <210> 314 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 314 gaaggtcgga gtcaacggat tgatcgatac acagatgtac atggc 45 <210> 315 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 315 gtctggctat catgacaaaa gca 23 <210> 316 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 316 gaaggtgacc aagttcatgc tagaaacttg ccaacccttt aacagata 48 <210> 317 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 317 gaaggtcgga gtcaacggat tgaaacttgc caacccttta acagatg 47 <210> 318 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 318 tccagaactt cttaagaaat gacagagaaa 30 <210> 319 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 319 gaaggtgacc aagttcatgc tggggcgcca aagagaggt 39 <210> 320 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 320 gaaggtcgga gtcaacggat tggggcgcca aagagaggc 39 <210> 321 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 321 aggtaggcgt cccctagttt 20 <210> 322 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 322 gaaggtgacc aagttcatgc ttttagaaat gattctcctc tgaccc 46 <210> 323 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 323 gaaggtcgga gtcaacggat tgttttagaa atgattctcc tctgacca 48 <210> 324 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 324 ctctcttctt tccctaaata ttaattgatt 30 <210> 325 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 325 gaaggtgacc aagttcatgc ttctttgctc tcttgactct tacac 45 <210> 326 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 326 gaaggtcgga gtcaacggat ttctttgctc tcttgactct tacat 45 <210> 327 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 327 tcctctattg aaggtcaatg gga 23 <210> 328 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 328 gaaggtgacc aagttcatgc taattcaggt acatactgcc acc 43 <210> 329 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 329 gaaggtcgga gtcaacggat taattcaggt acatactgcc act 43 <210> 330 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 330 tcgatcgttt ctcaacagcc 20 <210> 331 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 331 gaaggtgacc aagttcatgc ttgaagaaac cattgctctc tcg 43 <210> 332 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 332 gaaggtcgga gtcaacggat ttgaagaaac cattgctctc tca 43 <210> 333 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 333 ctcgaatcga gctctcatag aa 22 <210> 334 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 334 gaaggtgacc aagttcatgc tgtgtttaag ttgactacaa aaaccctca 49 <210> 335 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 335 gaaggtcgga gtcaacggat tgtttaagtt gactacaaaa accctcg 47 <210> 336 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 336 ggttatattt gttaggtata tgtattagtt 30 <210> 337 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 337 gaaggtgacc aagttcatgc tgcttacttt tagggattca aactgc 46 <210> 338 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 338 gaaggtcgga gtcaacggat tgcttacttt tagggattca aactgt 46 <210> 339 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 339 gggagagcgt tgtagcagtt 20 <210> 340 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 340 gaaggtgacc aagttcatgc tatgcctgat acatgaccca ag 42 <210> 341 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 341 gaaggtcgga gtcaacggat tatgcctgat acatgaccca aa 42 <210> 342 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 342 tcttagctcc cataaattgc ca 22 <210> 343 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 343 gaaggtgacc aagttcatgc tggaagaggg gtcagccgt 39 <210> 344 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 344 gaaggtcgga gtcaacggat tggaagaggg gtcagccgc 39 <210> 345 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 345 acgctagaaa ccgtcacacc 20 <210> 346 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 346 gaaggtgacc aagttcatgc ttttgctttt agatattcgc tggtac 46 <210> 347 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 347 gaaggtcgga gtcaacggat ttttgctttt agatattcgc tggtat 46 <210> 348 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 348 cttcgagtcg ggcagcttag 20 <210> 349 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 349 gaaggtgacc aagttcatgc tttgaaatat gccatattcg ccg 43 <210> 350 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 350 gaaggtcgga gtcaacggat tttgaaatat gccatattcg ccc 43 <210> 351 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 351 acatcaaaga ggtcgtactg aaa 23 <210> 352 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 352 gaaggtgacc aagttcatgc ttacccgccc attagttttc ccc 43 <210> 353 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 353 gaaggtcgga gtcaacggat ttacccgccc attagttttc cct 43 <210> 354 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 354 actttacttc tactcgtttg tcaga 25 <210> 355 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 355 gaaggtgacc aagttcatgc tgcgacacca gagacgactg g 41 <210> 356 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 356 gaaggtcgga gtcaacggat tgcgacacca gagacgactg a 41 <210> 357 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 357 cacctctgac tcttcgtgcc 20 <210> 358 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 358 gaaggtgacc aagttcatgc taaattttag ggaacgtatg gtttga 46 <210> 359 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 359 gaaggtcgga gtcaacggat taaattttag ggaacgtatg gtttgg 46 <210> 360 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 360 acctaaagaa accagtgacc ct 22 <210> 361 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 361 gaaggtgacc aagttcatgc tatgtggacc aagaatgccc c 41 <210> 362 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 362 gaaggtcgga gtcaacggat tatgtggacc aagaatgccc t 41 <210> 363 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 363 ctcgggacct tcacattgag t 21 <210> 364 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 364 gaaggtgacc aagttcatgc tatgtccatg caacactaaa gccc 44 <210> 365 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 365 gaaggtcgga gtcaacggat tgtatgtcca tgcaacacta aagcct 46 <210> 366 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 366 tcactaaaga gagtggactt tgagttgtt 29 <210> 367 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 367 gaaggtgacc aagttcatgc ttgttgatac cgtcccaaag gttg 44 <210> 368 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 368 gaaggtcgga gtcaacggat ttgttgatac cgtcccaaag gtta 44 <210> 369 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 369 agcgaccgat ctcacgattt 20 <210> 370 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 370 gaaggtgacc aagttcatgc tagtatgcac atcacccacc g 41 <210> 371 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 371 gaaggtcgga gtcaacggat tagtatgcac atcacccacc a 41 <210> 372 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 372 agctgagtca ctggaggcta 20 <210> 373 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 373 gaaggtgacc aagttcatgc tacaaaccag gtaagaagga gtc 43 <210> 374 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 374 gaaggtcgga gtcaacggat tacaaaccag gtaagaagga gtt 43 <210> 375 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 375 tcaaacccct catctggagc 20 <210> 376 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 376 gaaggtgacc aagttcatgc tacagttgca ggaaggaagt c 41 <210> 377 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 377 gaaggtcgga gtcaacggat tacagttgca ggaaggaagt g 41 <210> 378 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 378 ggtttccgcc acttagagga 20 <210> 379 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 379 gaaggtgacc aagttcatgc taaagaacgg gttggtaagt gc 42 <210> 380 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 380 gaaggtcgga gtcaacggat taaagaacgg gttggtaagt gt 42 <210> 381 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 381 tggccttatc aatctcggcg 20 <210> 382 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 382 gaaggtgacc aagttcatgc ttgacaaccg agttcctccc a 41 <210> 383 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 383 gaaggtcgga gtcaacggat ttgacaaccg agttcctccc g 41 <210> 384 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 384 agcatcagtc aattgtgtgt ga 22 <210> 385 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 385 gaaggtgacc aagttcatgc tcccctcccc acaacaaact 40 <210> 386 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 386 gaaggtcgga gtcaacggat tcccctcccc acaacaaacc 40 <210> 387 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 387 ggtcgtcaag gtggtttttc g 21 <210> 388 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 388 gaaggtgacc aagttcatgc tctcgatgtg ttattccact tgc 43 <210> 389 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 389 gaaggtcgga gtcaacggat tcctctcgat gtgttattcc acttgt 46 <210> 390 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 390 tggtatgaca gggcagccag ca 22 <210> 391 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 391 gaaggtgacc aagttcatgc tttttatgtc ttagggctcg aagt 44 <210> 392 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 392 gaaggtcgga gtcaacggat tttttatgtc ttagggctcg aagc 44 <210> 393 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 393 ttgtgagaca gcccaatcgg 20 <210> 394 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 394 gaaggtgacc aagttcatgc tcattaggtt tcaccccgat ca 42 <210> 395 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 395 gaaggtcgga gtcaacggat tcattaggtt tcaccccgat cg 42 <210> 396 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 396 ctggaggacc cgatcaacac 20 <210> 397 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 397 gaaggtgacc aagttcatgc tgatgtctcg taggaagact cgg 43 <210> 398 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 398 gaaggtcgga gtcaacggat tagatgtctc gtaggaagac tcga 44 <210> 399 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 399 tgtgggattt catacagatg cctaatgat 29 <210> 400 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 400 gaaggtgacc aagttcatgc tgagggaaag cggattgaca g 41 <210> 401 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 401 gaaggtcgga gtcaacggat tgagggaaag cggattgaca a 41 <210> 402 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 402 gaaagggaaa gatcgaatgc g 21 <210> 403 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 403 gaaggtgacc aagttcatgc tcgtcattga cttcgcttgt cat 43 <210> 404 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 404 gaaggtcgga gtcaacggat tcgtcattga cttcgcttgt cag 43 <210> 405 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 405 ggatatggtc gtctgaaacc ga 22 <210> 406 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 406 gaaggtgacc aagttcatgc tcgaaggtat tgcgcttaat tca 43 <210> 407 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 407 gaaggtcgga gtcaacggat tcgaaggtat tgcgcttaat tcg 43 <210> 408 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 408 gctccattga tgttctaaac ctagc 25 <210> 409 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 409 gaaggtgacc aagttcatgc tccacggaat ggcaagtctg g 41 <210> 410 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 410 gaaggtcgga gtcaacggat tccacggaat ggcaagtctg a 41 <210> 411 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 411 aagattgtca tcttgagcct aaatt 25 <210> 412 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 412 gaaggtgacc aagttcatgc tcatgtgcga cgttagtgac tatg 44 <210> 413 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 413 gaaggtcgga gtcaacggat tcatgtgcga cgttagtgac tata 44 <210> 414 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 414 acctcttggc ccaacatagc 20 <210> 415 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 415 gaaggtgacc aagttcatgc tggcttccac gaccaaggat g 41 <210> 416 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 416 gaaggtcgga gtcaacggat tggcttccac gaccaaggat t 41 <210> 417 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 417 acccccaacg tatttaggac ac 22 <210> 418 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 418 gaaggtgacc aagttcatgc tagactagct gccatagtaa gtaaag 46 <210> 419 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 419 gaaggtcgga gtcaacggat tagactagct gccatagtaa gtaaac 46 <210> 420 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 420 gtcagctcgg ttgtactaag ct 22 <210> 421 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 421 gaaggtgacc aagttcatgc tggaaaaatg gcgatgtgac gg 42 <210> 422 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 422 gaaggtcgga gtcaacggat tggaaaaatg gcgatgtgac ga 42 <210> 423 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 423 gagaaatgat tcgtccgcgc 20 <210> 424 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 424 gaaggtgacc aagttcatgc taactaagtc gccattcaca gg 42 <210> 425 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 425 gaaggtcgga gtcaacggat taactaagtc gccattcaca ga 42 <210> 426 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 426 tggggtcagc acaattagga t 21 <210> 427 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 427 gaaggtgacc aagttcatgc tgtacgactc ggagacaggt t 41 <210> 428 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 428 gaaggtcgga gtcaacggat tgtacgactc ggagacaggt c 41 <210> 429 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 429 acactttgcg ttgcaccatc 20 <210> 430 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 430 gaaggtgacc aagttcatgc tatccgtacc gtggtgatag a 41 <210> 431 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 431 gaaggtcgga gtcaacggat tatccgtacc gtggtgatag g 41 <210> 432 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 432 aaactcttct tccccaacgc t 21 <210> 433 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 433 gaaggtgacc aagttcatgc tctcaccggt gggagcaca 39 <210> 434 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 434 gaaggtcgga gtcaacggat tctcaccggt gggagcacc 39 <210> 435 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 435 gcccgcaccc gaaaaatg 18 <210> 436 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 436 gaaggtgacc aagttcatgc tctcactcgc gtgtgtaact a 41 <210> 437 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 437 gaaggtcgga gtcaacggat tctcactcgc gtgtgtaact g 41 <210> 438 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 438 atgttccact ccacatgcga 20 <210> 439 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 439 gaaggtgacc aagttcatgc taggatttcg aagtcatacc cg 42 <210> 440 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 440 gaaggtcgga gtcaacggat taggatttcg aagtcatacc cc 42 <210> 441 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 441 ctgaccagaa ggcttggaaa t 21 <210> 442 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 442 gaaggtgacc aagttcatgc tacatctctt cagcaagtag tgt 43 <210> 443 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 443 gaaggtcgga gtcaacggat tacatctctt cagcaagtag tgc 43 <210> 444 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 444 attagtaact gctaactgga ggtaa 25 <210> 445 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 445 gaaggtgacc aagttcatgc ttccttcatg acagtggaac cat 43 <210> 446 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 446 gaaggtcgga gtcaacggat ttccttcatg acagtggaac cac 43 <210> 447 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 447 tggggagaat gtatactaac tgc 23 <210> 448 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 448 gaaggtgacc aagttcatgc tgatgtgtat gttcatatag gaattgtcg 49 <210> 449 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 449 gaaggtcgga gtcaacggat tatgatgtgt atgttcatat aggaattgtc a 51 <210> 450 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 450 tttccataaa gttgctcgat tgcaaggat 29 <210> 451 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 451 gaaggtgacc aagttcatgc tgctgctctt agtagtcctg ataata 46 <210> 452 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 452 gaaggtcgga gtcaacggat tgctgctctt agtagtcctg ataatg 46 <210> 453 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 453 cggctggggg attcgtttat 20 <210> 454 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 454 gaaggtgacc aagttcatgc tactgacgaa agcagccaga g 41 <210> 455 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 455 gaaggtcgga gtcaacggat tactgacgaa agcagccaga c 41 <210> 456 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 456 ctgaccgtaa ccgactctgt 20 <210> 457 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 457 gaaggtgacc aagttcatgc tgtccacctc ccctcaccg 39 <210> 458 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 458 gaaggtcgga gtcaacggat tgtccacctc ccctcacca 39 <210> 459 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 459 gggtgctttg caataacagg a 21 <210> 460 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 460 gaaggtgacc aagttcatgc ttgatttgat gtaagggttg actagg 46 <210> 461 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 461 gaaggtcgga gtcaacggat ttgatttgat gtaagggttg actaga 46 <210> 462 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 462 accaatacag ttagacccaa tga 23 <210> 463 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 463 gaaggtgacc aagttcatgc tggttgattc ttacagtttc cgct 44 <210> 464 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 464 gaaggtcgga gtcaacggat tggttgattc ttacagtttc cgcc 44 <210> 465 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 465 cgtttctcta atccccaccc a 21 <210> 466 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 466 gaaggtgacc aagttcatgc tcataagtgc tgcctcctgg ta 42 <210> 467 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 467 gaaggtcgga gtcaacggat tcataagtgc tgcctcctgg tg 42 <210> 468 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 468 gacagaagct aaaactctca gcc 23 <210> 469 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 469 gaaggtgacc aagttcatgc tcacgcgtca tcatcacgtt g 41 <210> 470 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 470 gaaggtcgga gtcaacggat tcacgcgtca tcatcacgtt t 41 <210> 471 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 471 caccatcgat gatcacatgc a 21 <210> 472 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 472 gaaggtgacc aagttcatgc ttgtccaatt ggcggctgta c 41 <210> 473 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 473 gaaggtcgga gtcaacggat ttgtccaatt ggcggctgta a 41 <210> 474 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 474 gcttttgtcc atttagtccc ca 22 <210> 475 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 475 gaaggtgacc aagttcatgc tgccctatag gatcaatcac actacc 46 <210> 476 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 476 gaaggtcgga gtcaacggat tgccctatag gatcaatcac actact 46 <210> 477 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 477 actcctcgtg gttcacaaga 20 <210> 478 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 478 gaaggtgacc aagttcatgc tccctagctt ccgagacaga t 41 <210> 479 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 479 gaaggtcgga gtcaacggat tccctagctt ccgagacaga a 41 <210> 480 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 480 tggattgatc ggatggggtg 20 <210> 481 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 481 gaaggtgacc aagttcatgc tgcaaatgct agacatatcc cgac 44 <210> 482 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 482 gaaggtcgga gtcaacggat tgcaaatgct agacatatcc cgat 44 <210> 483 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 483 acgtcgtgtc gagagatctt 20 <210> 484 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 484 gaaggtgacc aagttcatgc taccccgtac agtatgtggc c 41 <210> 485 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 485 gaaggtcgga gtcaacggat tgtaccccgt acagtatgtg gca 43 <210> 486 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 486 ttctatggtg gcaaattgca aggaagtaa 29 <210> 487 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 487 gaaggtgacc aagttcatgc tgcaaaccag gtccacgcaa 40 <210> 488 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 488 gaaggtcgga gtcaacggat tgcaaaccag gtccacgcag 40 <210> 489 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 489 atggtggacg tgcttctcag 20 <210> 490 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 490 gaaggtgacc aagttcatgc tccacgtggc agcattggag 40 <210> 491 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 491 gaaggtcgga gtcaacggat tccacgtggc agcattggaa 40 <210> 492 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 492 taatctgccc ccgaggtgta 20 <210> 493 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 493 gaaggtgacc aagttcatgc tgggccacac gattcctgc 39 <210> 494 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 494 gaaggtcgga gtcaacggat tgggccacac gattcctgg 39 <210> 495 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 495 tgaatgtgga gcctcatgtc a 21 <210> 496 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 496 gaaggtgacc aagttcatgc taagaacctc aaaacttttg tatactcaaa c 51 <210> 497 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 497 gaaggtcgga gtcaacggat tgaaagaacc tcaaaacttt tgtatactca aat 53 <210> 498 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 498 cgcttatcaa agcttgcctc gacta 25 <210> 499 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 499 gaaggtgacc aagttcatgc tctgttcatc gacgacggca g 41 <210> 500 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 500 gaaggtcgga gtcaacggat tctgttcatc gacgacggca a 41 <210> 501 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 501 tcctagccgt ccctcgatat 20 <210> 502 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 502 gaaggtgacc aagttcatgc tcatgcgatc aattcccaac ca 42 <210> 503 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 503 gaaggtcgga gtcaacggat tcatgcgatc aattcccaac cg 42 <210> 504 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 504 tgtctgtcat ctttgttccc ct 22 <210> 505 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 505 gaaggtgacc aagttcatgc tcttatgatt gccaacaata ttcctgt 47 <210> 506 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 506 gaaggtcgga gtcaacggat tcttatgatt gccaacaata ttcctgc 47 <210> 507 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 507 caaaagctta aattagtggg tgaaggcaa 29 <210> 508 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 508 gaaggtgacc aagttcatgc tgccgcccaa gttcaactcc 40 <210> 509 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 509 gaaggtcgga gtcaacggat tgccgcccaa gttcaactct 40 <210> 510 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 510 cggttgactt tgacggcaag 20 <210> 511 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 511 gaaggtgacc aagttcatgc tagacgccaa atcacgagac g 41 <210> 512 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 512 gaaggtcgga gtcaacggat tagacgccaa atcacgagac t 41 <210> 513 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 513 ggattaggtc gtttggcagt 20 <210> 514 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 514 gaaggtgacc aagttcatgc ttcgtattgt tcatatttgc agatcg 46 <210> 515 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 515 gaaggtcgga gtcaacggat ttcgtattgt tcatatttgc agatca 46 <210> 516 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 516 acagggagga ttaaacttgt ga 22 <210> 517 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 517 gaaggtgacc aagttcatgc tccaaccgtg agacattgag ct 42 <210> 518 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 518 gaaggtcgga gtcaacggat tccaaccgtg agacattgag cc 42 <210> 519 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 519 actaacatgg tgcactttca ct 22 <210> 520 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 520 gaaggtgacc aagttcatgc tggatttgct cgatgtcaaa gcg 43 <210> 521 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 521 gaaggtcgga gtcaacggat tggatttgct cgatgtcaaa gca 43 <210> 522 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 522 atacttccga ccctcatcgc 20 <210> 523 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 523 gaaggtgacc aagttcatgc tagacacgtt gccatgagtt at 42 <210> 524 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 524 gaaggtcgga gtcaacggat tagacacgtt gccatgagtt aa 42 <210> 525 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 525 tcgtattgtt cggtaagcta cc 22 <210> 526 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 526 gaaggtgacc aagttcatgc tgttgccgct ctgctcgatc 40 <210> 527 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 527 gaaggtcgga gtcaacggat tgttgccgct ctgctcgata 40 <210> 528 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 528 gagccaatct gagtgacgag t 21 <210> 529 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 529 gaaggtgacc aagttcatgc tcgagcttgt ctggcgatcg 40 <210> 530 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 530 gaaggtcgga gtcaacggat tcgagcttgt ctggcgatca 40 <210> 531 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 531 tcaataacta cgatcgccag aca 23 <210> 532 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 532 gaaggtgacc aagttcatgc tagctaactg acggtccggg 40 <210> 533 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 533 gaaggtcgga gtcaacggat tagctaactg acggtccgga 40 <210> 534 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 534 acaatcttac ttgcgcagga 20 <210> 535 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 535 gaaggtgacc aagttcatgc tgcttatgac caacgcgcca t 41 <210> 536 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 536 gaaggtcgga gtcaacggat tgcttatgac caacgcgcca g 41 <210> 537 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 537 gggttaaaaa gcggtcgtgg 20 <210> 538 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 538 gaaggtgacc aagttcatgc tgttagttaa tcttaaatcg ttggagctca 50 <210> 539 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 539 gaaggtcgga gtcaacggat tagttaatct taaatcgttg gagctcg 47 <210> 540 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 540 tagtgtggga acctaatgga ccaataaat 29 <210> 541 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 541 gaaggtgacc aagttcatgc ttcaccattt gcagacgacg c 41 <210> 542 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 542 gaaggtcgga gtcaacggat ttcaccattt gcagacgacg t 41 <210> 543 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 543 agcttttgtt cacatgcgag g 21 <210> 544 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 544 gaaggtgacc aagttcatgc taatgggtta tcttcaacaa ctattaaaag c 51 <210> 545 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 545 gaaggtcgga gtcaacggat taaaatgggt tatcttcaac aactattaaa agt 53 <210> 546 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 546 gagtacatgc caaacactac aggaaataa 29 <210> 547 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 547 gaaggtgacc aagttcatgc ttgagaggat tcccattggc tc 42 <210> 548 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 548 gaaggtcgga gtcaacggat ttgagaggat tcccattggc tt 42 <210> 549 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 549 aagggcttgt tccttctcga 20 <210> 550 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 550 gaaggtgacc aagttcatgc tggtcgttgg tgtcaattga ct 42 <210> 551 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 551 gaaggtcgga gtcaacggat tggtcgttgg tgtcaattga cc 42 <210> 552 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 552 gaggtggata ttgtagaagg gca 23 <210> 553 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 553 gaaggtgacc aagttcatgc tgaatcaatg tgaagcggag taatgt 46 <210> 554 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 554 gaaggtcgga gtcaacggat tgaatcaatg tgaagcggag taatgg 46 <210> 555 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 555 cgacctgtcg ttcagcgt 18 <210> 556 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 556 gaaggtgacc aagttcatgc ttccaactca ctcaattgtg acttaca 47 <210> 557 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 557 gaaggtcgga gtcaacggat tccaactcac tcaattgtga cttacc 46 <210> 558 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 558 gcctccaagt gttgagacgt acaaa 25 <210> 559 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 559 gaaggtgacc aagttcatgc taaaaacagc tttcgtcagt ctcg 44 <210> 560 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 560 gaaggtcgga gtcaacggat taaaaacagc tttcgtcagt ctca 44 <210> 561 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 561 tttgtttcga tccattctga cca 23 <210> 562 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 562 gaaggtgacc aagttcatgc ttcgaagagg taggttgcgg a 41 <210> 563 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 563 gaaggtcgga gtcaacggat ttcgaagagg taggttgcgg g 41 <210> 564 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 564 ctttcctctt gccttgggga 20 <210> 565 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 565 gaaggtgacc aagttcatgc taggagaata gggtcgactg tcc 43 <210> 566 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 566 gaaggtcgga gtcaacggat taaaaggaga atagggtcga ctgtct 46 <210> 567 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 567 caaatcactc ttcaaagagc aagagcatt 29 <210> 568 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 568 gaaggtgacc aagttcatgc tctacgcagc ccaaaaacgt ag 42 <210> 569 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 569 gaaggtcgga gtcaacggat tctacgcagc ccaaaaacgt at 42 <210> 570 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 570 cgacactgtt gttgtgcgtt 20 <210> 571 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 571 gaaggtgacc aagttcatgc taacttacac ttgaagctat ggtatg 46 <210> 572 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 572 gaaggtcgga gtcaacggat taacttacac ttgaagctat ggtatc 46 <210> 573 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 573 gaaatggaat gattgcttca ctct 24 <210> 574 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 574 gaaggtgacc aagttcatgc tcataccatc tagttggtgc atctg 45 <210> 575 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 575 gaaggtcgga gtcaacggat tgcataccat ctagttggtg catcta 46 <210> 576 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 576 cagaaaacaa aggcctacag ctattgaaa 29 <210> 577 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 577 gaaggtgacc aagttcatgc tttggctcct cgcggatgc 39 <210> 578 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 578 gaaggtcgga gtcaacggat tttggctcct cgcggatgt 39 <210> 579 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 579 tggatcctat ttccgcttag ca 22 <210> 580 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 580 gaaggtgacc aagttcatgc tgttgtcctc tatcttcgtt gct 43 <210> 581 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 581 gaaggtcgga gtcaacggat tgttgtcctc tatcttcgtt gcc 43 <210> 582 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 582 ctagcagaga cgtggtggtc 20 <210> 583 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 583 gaaggtgacc aagttcatgc taataatttt gaaaacggaa aaagcctact g 51 <210> 584 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 584 gaaggtcgga gtcaacggat taaaataatt ttgaaaacgg aaaaagccta cta 53 <210> 585 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 585 cgcgtattga ttgggacagt ttttgtatt 29 <210> 586 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 586 gaaggtgacc aagttcatgc tggttaggtt tagtgcaatc taggtc 46 <210> 587 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 587 gaaggtcgga gtcaacggat tggttaggtt tagtgcaatc taggtt 46 <210> 588 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 588 tgaaaactcg tattgaaacg cca 23 <210> 589 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 589 gaaggtgacc aagttcatgc tagcttgcgt ggaagttagg t 41 <210> 590 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 590 gaaggtcgga gtcaacggat tagcttgcgt ggaagttagg c 41 <210> 591 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 591 agtctggctt caattcgcct 20 <210> 592 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 592 gaaggtgacc aagttcatgc tgtcatgccc cctagaacgc t 41 <210> 593 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 593 gaaggtcgga gtcaacggat tgtcatgccc cctagaacgc a 41 <210> 594 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 594 ctgaaaattg cccaggtcgc 20 <210> 595 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 595 gaaggtgacc aagttcatgc tacaaccata agtcgagatc ttcg 44 <210> 596 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 596 gaaggtcgga gtcaacggat tacaaccata agtcgagatc ttca 44 <210> 597 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 597 gaagataaca atggcggccg 20 <210> 598 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 598 gaaggtgacc aagttcatgc tgatagttga tgtcaagaat tgattgtcta aaa 53 <210> 599 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 599 gaaggtcgga gtcaacggat tagttgatgt caagaattga ttgtctaaac 50 <210> 600 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 600 caccttatca agttcaatca gtcaagcaa 29 <210> 601 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 601 gaaggtgacc aagttcatgc tgccgattga ttggacctca cc 42 <210> 602 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 602 gaaggtcgga gtcaacggat tctgccgatt gattggacct cact 44 <210> 603 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 603 gatgccatgt gtctttgtcc atttcttat 29 <210> 604 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 604 gaaggtgacc aagttcatgc ttcattttct aagtcagatt cccgc 45 <210> 605 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 605 gaaggtcgga gtcaacggat ttcattttct aagtcagatt cccgt 45 <210> 606 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 606 aagcattggc cgagctactt 20 <210> 607 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 607 gaaggtgacc aagttcatgc tgacatacct acggtatagg ggt 43 <210> 608 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 608 gaaggtcgga gtcaacggat tgacatacct acggtatagg ggc 43 <210> 609 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 609 cggaggacta ttcattgccg t 21 <210> 610 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 610 gaaggtgacc aagttcatgc tgggtggcgt tgaaccaagt 40 <210> 611 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 611 gaaggtcgga gtcaacggat tgggtggcgt tgaaccaagc 40 <210> 612 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 612 gaagacagct gcaccgtgta 20 <210> 613 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 613 gaaggtgacc aagttcatgc taattgatga aacttataag tgcaagtgg 49 <210> 614 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 614 gaaggtcgga gtcaacggat tgtaattgat gaaacttata agtgcaagtg a 51 <210> 615 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 615 cgttgctcta atctccattc acaaatcat 29 <210> 616 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 616 gaaggtgacc aagttcatgc tacatcggaa tcaatcccca ca 42 <210> 617 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 617 gaaggtcgga gtcaacggat tacatcggaa tcaatcccca cg 42 <210> 618 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 618 gttccttact gcccttgcct 20 <210> 619 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 619 gaaggtgacc aagttcatgc tcaccgggaa agataaacgc g 41 <210> 620 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 620 gaaggtcgga gtcaacggat tcaccgggaa agataaacgc a 41 <210> 621 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 621 attcatgggc tcaacttcag c 21 <210> 622 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 622 gaaggtgacc aagttcatgc tgcactgttg gaatcctaca ctca 44 <210> 623 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 623 gaaggtcgga gtcaacggat tgcactgttg gaatcctaca ctct 44 <210> 624 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 624 ttcaccgtca accatggcat 20 <210> 625 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 625 gaaggtgacc aagttcatgc tccattatcc tgacccagct tc 42 <210> 626 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 626 gaaggtcgga gtcaacggat tccattatcc tgacccagct tt 42 <210> 627 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 627 cagtgcggca gttcaattcc 20 <210> 628 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 628 gaaggtgacc aagttcatgc tacacgattg tttagatttg gttaccc 47 <210> 629 <211> 47 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 629 gaaggtcgga gtcaacggat tacacgattg tttagatttg gttacct 47 <210> 630 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 630 tggggtaacg atcatttagt caaatctaaa 30 <210> 631 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 631 gaaggtgacc aagttcatgc tagtcactca caaacatgac gga 43 <210> 632 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 632 gaaggtcgga gtcaacggat tagtcactca caaacatgac ggg 43 <210> 633 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 633 tgatttgcgt gcttctgaag 20 <210> 634 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 634 gaaggtgacc aagttcatgc tcaccattgg gcttggaatc atat 44 <210> 635 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 635 gaaggtcgga gtcaacggat tcaccattgg gcttggaatc atac 44 <210> 636 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 636 acggacaaaa gaaaagcgag c 21 <210> 637 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 637 gaaggtgacc aagttcatgc ttgcaggttt cttgcctcag g 41 <210> 638 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 638 gaaggtcgga gtcaacggat ttgcaggttt cttgcctcag a 41 <210> 639 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 639 tttcagctgc tctctgcctc 20 <210> 640 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 640 gaaggtgacc aagttcatgc tacattattg gtgcctacat ccg 43 <210> 641 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 641 gaaggtcgga gtcaacggat tacattattg gtgcctacat cct 43 <210> 642 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 642 agttatctca cgtgcatgtg gt 22 <210> 643 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 643 gaaggtgacc aagttcatgc tggtccacag cgggtatgat t 41 <210> 644 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 644 gaaggtcgga gtcaacggat tggtccacag cgggtatgat a 41 <210> 645 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 645 tgctgtacca agatagtaga cca 23 <210> 646 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 646 gaaggtgacc aagttcatgc ttcttaaaac acgatcctac agtcac 46 <210> 647 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 647 gaaggtcgga gtcaacggat ttcttaaaac acgatcctac agtcaa 46 <210> 648 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 648 gatctcaatg taacgtaccc tgt 23 <210> 649 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 649 gaaggtgacc aagttcatgc ttggtactgc gtgcatttcc t 41 <210> 650 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 650 gaaggtcgga gtcaacggat ttggtactgc gtgcatttcc c 41 <210> 651 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 651 gaacgagaag gaccgtggtc 20 <210> 652 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 652 gaaggtgacc aagttcatgc ttgtttggaa tacattgggt cgtg 44 <210> 653 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 653 gaaggtcgga gtcaacggat ttgtttggaa tacattgggt cgtc 44 <210> 654 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 654 agcacattcc caaacccgat 20 <210> 655 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 655 gaaggtgacc aagttcatgc tctctggaac ccggtccga 39 <210> 656 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 656 gaaggtcgga gtcaacggat tctctggaac ccggtccgg 39 <210> 657 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 657 agtcaacatc aggcatgtgt c 21 <210> 658 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 658 gaaggtgacc aagttcatgc ttggacctag gtagagcacg g 41 <210> 659 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 659 gaaggtcgga gtcaacggat ttggacctag gtagagcacg a 41 <210> 660 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 660 atgcaggtgg ctaggtgaag 20 <210> 661 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 661 gaaggtgacc aagttcatgc tgtgacctgc ctaatttggg taag 44 <210> 662 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 662 gaaggtcgga gtcaacggat tgtgacctgc ctaatttggg taat 44 <210> 663 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 663 aatattaagc tggccacaaa gc 22 <210> 664 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 664 gaaggtgacc aagttcatgc taacctcttt ttttaaccta tttttggcaa tttt 54 <210> 665 <211> 52 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 665 gaaggtcgga gtcaacggat tcctcttttt ttaacctatt tttggcaatt tc 52 <210> 666 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 666 catgtttgct tcagatgttt gagttcctt 29 <210> 667 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 667 gaaggtgacc aagttcatgc tattgcacgc gtctcctcgc 40 <210> 668 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 668 gaaggtcgga gtcaacggat tattgcacgc gtctcctcgt 40 <210> 669 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 669 tgtgccgtgg actacaaaag 20 <210> 670 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 670 gaaggtgacc aagttcatgc tactcggggt ggccaatttt 40 <210> 671 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 671 gaaggtcgga gtcaacggat tactcggggt ggccaatttg 40 <210> 672 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 672 cgtagaaact tacaggagca tct 23 <210> 673 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 673 gaaggtgacc aagttcatgc tgaaataatg agtccaagct cgtc 44 <210> 674 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 674 gaaggtcgga gtcaacggat tgaaataatg agtccaagct cgtt 44 <210> 675 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 675 atttgcttgg tcggcattgg 20 <210> 676 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 676 gaaggtgacc aagttcatgc tttttgctgc gaatttggca g 41 <210> 677 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 677 gaaggtcgga gtcaacggat tttttgctgc gaatttggca t 41 <210> 678 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 678 aaaccccatc agacaccgtc 20 <210> 679 <211> 50 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 679 gaaggtgacc aagttcatgc taattggttt aaggtccaag ttataaactg 50 <210> 680 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 680 gaaggtcgga gtcaacggat tcaattggtt taaggtccaa gttataaact a 51 <210> 681 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 681 caccgtctca ttagctcgag agaaa 25 <210> 682 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 682 gaaggtgacc aagttcatgc taaaatagaa acaacaccct gctt 44 <210> 683 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 683 gaaggtcgga gtcaacggat taaaatagaa acaacaccct gctc 44 <210> 684 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 684 ttggcgggtt aaacttgtgt 20 <210> 685 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 685 gaaggtgacc aagttcatgc tcggtcgtcg cttgtctctc 40 <210> 686 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 686 gaaggtcgga gtcaacggat tcggtcgtcg cttgtctctt 40 <210> 687 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 687 tgtgggcgac attgaaagtt 20 <210> 688 <211> 48 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 688 gaaggtgacc aagttcatgc taatcttcat atgtaggcta aacgatcc 48 <210> 689 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 689 gaaggtcgga gtcaacggat tataaatctt catatgtagg ctaaacgatc t 51 <210> 690 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 690 gtcgaagaac ctgcatgagt tgagat 26 <210> 691 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 691 gaaggtgacc aagttcatgc ttttgagaag tgcaacgacc g 41 <210> 692 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 692 gaaggtcgga gtcaacggat ttttgagaag tgcaacgacc a 41 <210> 693 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 693 acgatcaaca tcatcgcact 20 <210> 694 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 694 gaaggtgacc aagttcatgc tacgagatca tgcacacagg c 41 <210> 695 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 695 gaaggtcgga gtcaacggat tacgagatca tgcacacagg g 41 <210> 696 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 696 cctgtgtgct tctgggtctg 20 <210> 697 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 697 gaaggtgacc aagttcatgc tgcatattaa tttgtatctt gaatttgctc g 51 <210> 698 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 698 gaaggtcgga gtcaacggat tgcatattaa tttgtatctt gaatttgctc c 51 <210> 699 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 699 tacaaattac agcacgcttg gaggaattt 29 <210> 700 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 700 gaaggtgacc aagttcatgc tggttagcta tagcaactta acgaac 46 <210> 701 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 701 gaaggtcgga gtcaacggat tggttagcta tagcaactta acgaag 46 <210> 702 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 702 agcgtagaaa cattggttct gtc 23 <210> 703 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 703 gaaggtgacc aagttcatgc tgtccaggtt atttgtaagc ctttgg 46 <210> 704 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 704 gaaggtcgga gtcaacggat tgtccaggtt atttgtaagc ctttgc 46 <210> 705 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 705 tgggtattca taaaacttca cgaaaaccta 30 <210> 706 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 706 gaaggtgacc aagttcatgc tcgaaatgtg ggtgaggtca tga 43 <210> 707 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 707 gaaggtcgga gtcaacggat tcgaaatgtg ggtgaggtca tgg 43 <210> 708 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 708 tgtcgccaaa caatctccca 20 <210> 709 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 709 gaaggtgacc aagttcatgc taatagaggc gtggttgcga g 41 <210> 710 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 710 gaaggtcgga gtcaacggat taatagaggc gtggttgcga a 41 <210> 711 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 711 atagactcgg caaccacacc 20 <210> 712 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 712 gaaggtgacc aagttcatgc taccaccacc tcagatattt tcgat 45 <210> 713 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 713 gaaggtcgga gtcaacggat taccaccacc tcagatattt tcgag 45 <210> 714 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 714 agcgacaaat tggaggatac ga 22 <210> 715 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 715 gaaggtgacc aagttcatgc tacattcaaa tagcaaatga ctaacagatt c 51 <210> 716 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 716 gaaggtcgga gtcaacggat taatacattc aaatagcaaa tgactaacag attt 54 <210> 717 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 717 cctgtcattg ttgatgatgt ctttggtaa 29 <210> 718 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 718 gaaggtgacc aagttcatgc ttagtactgg cgacgtggaa c 41 <210> 719 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 719 gaaggtcgga gtcaacggat ttagtactgg cgacgtggaa t 41 <210> 720 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 720 gtcacttgag cctagatctc ca 22 <210> 721 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 721 gaaggtgacc aagttcatgc tgcctagcag atgcagagcg 40 <210> 722 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 722 gaaggtcgga gtcaacggat tgcctagcag atgcagagct 40 <210> 723 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 723 gagcacgtga acattaccag t 21 <210> 724 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 724 gaaggtgacc aagttcatgc tagcgaattg gatggtgata ttaaac 46 <210> 725 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 725 gaaggtcgga gtcaacggat tagcgaattg gatggtgata ttaaaa 46 <210> 726 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 726 ggtagcaagg ttccaaaatg ttg 23 <210> 727 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 727 gaaggtgacc aagttcatgc tcaatgcaat gcactgtggg t 41 <210> 728 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 728 gaaggtcgga gtcaacggat tcaatgcaat gcactgtggg c 41 <210> 729 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 729 tcctgaaaag aaggcagagg a 21 <210> 730 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 730 gaaggtgacc aagttcatgc tggtcaaggt aaaggtcgtg gt 42 <210> 731 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 731 gaaggtcgga gtcaacggat tggtcaaggt aaaggtcgtg ga 42 <210> 732 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 732 ggatgattaa aatgaccagc acg 23 <210> 733 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 733 gaaggtgacc aagttcatgc taggtcgttg gtccatttaa ttaca 45 <210> 734 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 734 gaaggtcgga gtcaacggat taggtcgttg gtccatttaa ttacg 45 <210> 735 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 735 ttgcttcagc gtgagtaggc 20 <210> 736 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 736 gaaggtgacc aagttcatgc ttcatccgtg gaagggtaaa ttc 43 <210> 737 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 737 gaaggtcgga gtcaacggat ttcatccgtg gaagggtaaa ttt 43 <210> 738 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 738 tggcaaaact aactgcgaga 20 <210> 739 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 739 gaaggtgacc aagttcatgc ttgtaccaac aaaaattcca ttcacc 46 <210> 740 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 740 gaaggtcgga gtcaacggat ttgtaccaac aaaaattcca ttcaca 46 <210> 741 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 741 aggttgtacg tgcgatctca a 21 <210> 742 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 742 gaaggtgacc aagttcatgc tcccctacaa atgggtctac aagaag 46 <210> 743 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 743 gaaggtcgga gtcaacggat tcccctacaa atgggtctac aagaaa 46 <210> 744 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 744 gcccttagaa actagtctcg c 21 <210> 745 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 745 gaaggtgacc aagttcatgc taacaatccc acacatgaaa gaaat 45 <210> 746 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 746 gaaggtcgga gtcaacggat taacaatccc acacatgaaa gaaag 45 <210> 747 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 747 tgtctgtttg ctctcaacag t 21 <210> 748 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 748 gaaggtgacc aagttcatgc tgcaagaaaa ttcaagaatc gatcg 45 <210> 749 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 749 gaaggtcgga gtcaacggat tgcaagaaaa ttcaagaatc gatcc 45 <210> 750 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 750 tgtctctcga cttcatttct tagaa 25 <210> 751 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 751 gaaggtgacc aagttcatgc tcctcttcaa cctcttcctt ccc 43 <210> 752 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 752 gaaggtcgga gtcaacggat tcctcttcaa cctcttcctt cct 43 <210> 753 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 753 aattggctat ggtagttaaa ggatg 25 <210> 754 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 754 gaaggtgacc aagttcatgc ttttaattta ggagccaaag tcagtc 46 <210> 755 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 755 gaaggtcgga gtcaacggat ttttaattta ggagccaaag tcagtt 46 <210> 756 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 756 cagcccaaat caccatgaac c 21 <210> 757 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 757 gaaggtgacc aagttcatgc tagctcacct gatctggaat cg 42 <210> 758 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 758 gaaggtcgga gtcaacggat tagctcacct gatctggaat ca 42 <210> 759 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 759 acatttggcg caaataaagg g 21 <210> 760 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 760 gaaggtgacc aagttcatgc tttgatttgc taaatgtagg acaaca 46 <210> 761 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 761 gaaggtcgga gtcaacggat tttgatttgc taaatgtagg acaacg 46 <210> 762 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 762 accatcttca cctccacttg aa 22 <210> 763 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 763 gaaggtgacc aagttcatgc tagtgttttc agaaggtgag gc 42 <210> 764 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 764 gaaggtcgga gtcaacggat tagtgttttc agaaggtgag gt 42 <210> 765 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 765 tggaggtggc aaattttaag tg 22 <210> 766 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 766 gaaggtgacc aagttcatgc ttgatgtgca tccaactcag tg 42 <210> 767 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 767 gaaggtcgga gtcaacggat ttgatgtgca tccaactcag ta 42 <210> 768 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 768 gccaactccc gatccacata 20 <210> 769 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 769 gaaggtgacc aagttcatgc tccacaacag ttcctaaagt tagagc 46 <210> 770 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 770 gaaggtcgga gtcaacggat tccacaacag ttcctaaagt tagagt 46 <210> 771 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 771 aaagttcttc cacgaatcgc c 21 <210> 772 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 772 gaaggtgacc aagttcatgc tagccctacg aaacaagtga gg 42 <210> 773 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 773 gaaggtcgga gtcaacggat tagccctacg aaacaagtga ga 42 <210> 774 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 774 actgacattg aagacccact t 21 <210> 775 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 775 gaaggtgacc aagttcatgc taagagtttc aaagagttgt tcactc 46 <210> 776 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 776 gaaggtcgga gtcaacggat taagagtttc aaagagttgt tcactt 46 <210> 777 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 777 ataggtgtgc tctctccatt ct 22 <210> 778 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 778 gaaggtgacc aagttcatgc tacaacatca aagtgagttg attcag 46 <210> 779 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 779 gaaggtcgga gtcaacggat tacaacatca aagtgagttg attcaa 46 <210> 780 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 780 gacatcgcgt ttgtagtggg 20 <210> 781 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 781 gaaggtgacc aagttcatgc tggaatagaa tgccttagct tcatca 46 <210> 782 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 782 gaaggtcgga gtcaacggat tggaatagaa tgccttagct tcatcg 46 <210> 783 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 783 caagcagatt gttgggcact a 21 <210> 784 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 784 gaaggtgacc aagttcatgc tacaatgcgt actcacccaa aac 43 <210> 785 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 785 gaaggtcgga gtcaacggat tacaatgcgt actcacccaa aat 43 <210> 786 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 786 atggagtttg ggcatatgat tgc 23 <210> 787 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 787 gaaggtgacc aagttcatgc tacactatgg tgggcatgtt cg 42 <210> 788 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 788 gaaggtcgga gtcaacggat tacactatgg tgggcatgtt ca 42 <210> 789 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 789 gtgtgtcaca tccaaagttg g 21 <210> 790 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 790 gaaggtgacc aagttcatgc ttcatcttta tgcggcatga tgt 43 <210> 791 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 791 gaaggtcgga gtcaacggat ttcatcttta tgcggcatga tgg 43 <210> 792 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 792 ggaaaaacgt tctgcaaact gc 22 <210> 793 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 793 gaaggtgacc aagttcatgc tttcaaaagt catgcaatcc ctct 44 <210> 794 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 794 gaaggtcgga gtcaacggat tttcaaaagt catgcaatcc ctcc 44 <210> 795 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 795 ggacgactat ttatacagct agga 24 <210> 796 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 796 gaaggtgacc aagttcatgc taagttccag gtccttcagt tgg 43 <210> 797 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 797 gaaggtcgga gtcaacggat taagttccag gtccttcagt tga 43 <210> 798 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 798 acatgctttc ttatcagacg aga 23 <210> 799 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 799 gaaggtgacc aagttcatgc tctaagttca tattcgttgg ccatag 46 <210> 800 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 800 gaaggtcgga gtcaacggat tctaagttca tattcgttgg ccatac 46 <210> 801 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 801 agaccgataa gtgcaggatc ac 22 <210> 802 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 802 gaaggtgacc aagttcatgc tcccattctc agaaacaaac taacac 46 <210> 803 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 803 gaaggtcgga gtcaacggat tcccattctc agaaacaaac taacat 46 <210> 804 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 804 acgacacatg gtttctccct 20 <210> 805 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 805 gaaggtgacc aagttcatgc tcaaaagagc cacgccccaa g 41 <210> 806 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 806 gaaggtcgga gtcaacggat tcaaaagagc cacgccccaa c 41 <210> 807 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 807 gctcaacaag gccacaagtg 20 <210> 808 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 808 gaaggtgacc aagttcatgc tacatctctg caacaaacaa ttcgg 45 <210> 809 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 809 gaaggtcgga gtcaacggat tacatctctg caacaaacaa ttcga 45 <210> 810 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 810 agtgtcatcg actaatgaac ca 22 <210> 811 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 811 gaaggtgacc aagttcatgc tggggcctaa caaaagactt gttt 44 <210> 812 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 812 gaaggtcgga gtcaacggat tggggcctaa caaaagactt gttg 44 <210> 813 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 813 aaagagctcg gggcaaacaa 20 <210> 814 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 814 gaaggtgacc aagttcatgc tgttgaaatt tctcattcaa gagcga 46 <210> 815 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 815 gaaggtcgga gtcaacggat tgttgaaatt tctcattcaa gagcgg 46 <210> 816 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 816 aaactcattt ttgaaaacat gtcgt 25 <210> 817 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 817 gaaggtgacc aagttcatgc tggagctatt tttggtcgat aacag 45 <210> 818 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 818 gaaggtcgga gtcaacggat tggagctatt tttggtcgat aacaa 45 <210> 819 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 819 agccaaaatt ctgctcgaga 20 <210> 820 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 820 gaaggtgacc aagttcatgc tacacgtgca tcatacatgt atca 44 <210> 821 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 821 gaaggtcgga gtcaacggat tacacgtgca tcatacatgt atcg 44 <210> 822 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 822 gtcatttcgt atgttcgtgt gt 22 <210> 823 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 823 gaaggtgacc aagttcatgc tacatatacc ctgacatcca gct 43 <210> 824 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 824 gaaggtcgga gtcaacggat tacatatacc ctgacatcca gcc 43 <210> 825 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 825 ggccgtgctt tgttatttct t 21 <210> 826 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 826 gaaggtgacc aagttcatgc taaacagatc aggaaacaca gtgg 44 <210> 827 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 827 gaaggtcgga gtcaacggat taaacagatc aggaaacaca gtga 44 <210> 828 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 828 tgatccctct gggcctctag 20 <210> 829 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 829 gaaggtgacc aagttcatgc ttgggaataa aagacaacat cacctg 46 <210> 830 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 830 gaaggtcgga gtcaacggat ttgggaataa aagacaacat caccta 46 <210> 831 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 831 agcaagtcta catctctgcc at 22 <210> 832 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 832 gaaggtgacc aagttcatgc tatggtttca atagttcaat cgaagt 46 <210> 833 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 833 gaaggtcgga gtcaacggat tatggtttca atagttcaat cgaagc 46 <210> 834 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 834 tggtcgttgc aatagaaatt ctc 23 <210> 835 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 835 gaaggtgacc aagttcatgc tacccatctg gagaatttgg tacc 44 <210> 836 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 836 gaaggtcgga gtcaacggat tacccatctg gagaatttgg taca 44 <210> 837 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 837 ctccagaagt aaaatttgtt ccagc 25 <210> 838 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 838 gaaggtgacc aagttcatgc taactaagga ttaagtacta ggcgac 46 <210> 839 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 839 gaaggtcgga gtcaacggat taactaagga ttaagtacta ggcgat 46 <210> 840 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 840 cttgaacctt ggagaccttc a 21 <210> 841 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 841 gaaggtgacc aagttcatgc tcctcgttta tcgccttcct taac 44 <210> 842 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 842 gaaggtcgga gtcaacggat tcctcgttta tcgccttcct taag 44 <210> 843 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 843 acacacacac atggttgttg a 21 <210> 844 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 844 gaaggtgacc aagttcatgc tcttagcttc ctgcatgtaa gaatc 45 <210> 845 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 845 gaaggtcgga gtcaacggat tcttagcttc ctgcatgtaa gaatt 45 <210> 846 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 846 cttggccttc cttcaccgat 20 <210> 847 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 847 gaaggtgacc aagttcatgc tacaattgtc aagcaaagca tcag 44 <210> 848 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 848 gaaggtcgga gtcaacggat tacaattgtc aagcaaagca tcaa 44 <210> 849 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 849 actcagtcca ctagcaactt cc 22 <210> 850 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 850 gaaggtgacc aagttcatgc tgttaaggaa ttaacaatgc gggtag 46 <210> 851 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 851 gaaggtcgga gtcaacggat tgttaaggaa ttaacaatgc gggtaa 46 <210> 852 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 852 ggatgagtct gctgaaggct 20 <210> 853 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 853 gaaggtgacc aagttcatgc tttatatcct aggcccaaat cacac 45 <210> 854 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 854 gaaggtcgga gtcaacggat tttatatcct aggcccaaat cacaa 45 <210> 855 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 855 acatgcattt ccaatgaagc ca 22 <210> 856 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 856 gaaggtgacc aagttcatgc ttgatcaaac catagaagga gagtta 46 <210> 857 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 857 gaaggtcgga gtcaacggat ttgatcaaac catagaagga gagttg 46 <210> 858 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 858 agctatcaca caccccaagc 20 <210> 859 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 859 gaaggtgacc aagttcatgc tccttgtcgt tcattgggac tatatt 46 <210> 860 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 860 gaaggtcgga gtcaacggat tccttgtcgt tcattgggac tatata 46 <210> 861 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 861 tccaagtctt caatctttca gacg 24 <210> 862 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 862 gaaggtgacc aagttcatgc tacaataaaa gccttttgca gcaga 45 <210> 863 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 863 gaaggtcgga gtcaacggat tacaataaaa gccttttgca gcagc 45 <210> 864 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 864 gcatgcttag cgttacagga 20 <210> 865 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 865 gaaggtgacc aagttcatgc tagcaaagga agcagtatac ctaagg 46 <210> 866 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 866 gaaggtcgga gtcaacggat tagcaaagga agcagtatac ctaaga 46 <210> 867 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 867 tgccctactg ttgtcatagt attaa 25 <210> 868 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 868 gaaggtgacc aagttcatgc ttgtcagact gaaggaatca atcatg 46 <210> 869 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 869 gaaggtcgga gtcaacggat ttgtcagact gaaggaatca atcata 46 <210> 870 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 870 ttaaggagtt gtgggagctg c 21 <210> 871 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 871 gaaggtgacc aagttcatgc tcttttctaa tacaggctac ctcacc 46 <210> 872 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 872 gaaggtcgga gtcaacggat tcttttctaa tacaggctac ctcaca 46 <210> 873 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 873 gaacatgatt gagatgtcaa agagt 25 <210> 874 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 874 gaaggtgacc aagttcatgc taccctgcca cggtacaga 39 <210> 875 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 875 gaaggtcgga gtcaacggat taccctgcca cggtacagt 39 <210> 876 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 876 aaaagcattg caccagccg 19 <210> 877 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 877 gaaggtgacc aagttcatgc tgactcgaat caatgatgca tcct 44 <210> 878 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 878 gaaggtcgga gtcaacggat tgactcgaat caatgatgca tccc 44 <210> 879 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 879 aaccaaccga catagagaaa aatt 24 <210> 880 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 880 gaaggtgacc aagttcatgc tctgttcaag accggaccgt c 41 <210> 881 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 881 gaaggtcgga gtcaacggat tctgttcaag accggaccgt a 41 <210> 882 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 882 caaatgcttg gcctcagtcg 20 <210> 883 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 883 gaaggtgacc aagttcatgc tggtttagcg tttagtgaaa gaaga 45 <210> 884 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 884 gaaggtcgga gtcaacggat tggtttagcg tttagtgaaa gaagg 45 <210> 885 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 885 agaaagacca aaatgcacag ga 22 <210> 886 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 886 gaaggtgacc aagttcatgc taaaagggaa gttgttcaaa ctcc 44 <210> 887 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 887 gaaggtcgga gtcaacggat taaaagggaa gttgttcaaa ctca 44 <210> 888 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 888 gaactacacc tattgctcag cg 22 <210> 889 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 889 gaaggtgacc aagttcatgc taccatttag agtgtggggg g 41 <210> 890 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 890 gaaggtcgga gtcaacggat taccatttag agtgtggggg a 41 <210> 891 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 891 tgtgggtttg gatctttgtg tt 22 <210> 892 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 892 gaaggtgacc aagttcatgc tctcatgggt ctcttcaacc tgt 43 <210> 893 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 893 gaaggtcgga gtcaacggat tctcatgggt ctcttcaacc tgg 43 <210> 894 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 894 tcttcccttc tgttgtcagc a 21 <210> 895 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 895 gaaggtgacc aagttcatgc tcacgaggaa caaaattggc attt 44 <210> 896 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 896 gaaggtcgga gtcaacggat tcacgaggaa caaaattggc attc 44 <210> 897 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 897 atcagaaaat tttagcctgg cct 23 <210> 898 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 898 gaaggtgacc aagttcatgc tgtttgtggc atttcaatgt atacgc 46 <210> 899 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 899 gaaggtcgga gtcaacggat tgtttgtggc atttcaatgt atacgt 46 <210> 900 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 900 gtgagcatcg caacactaca a 21 <210> 901 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 901 gaaggtgacc aagttcatgc ttcgaaaaat tgaagagttc cggc 44 <210> 902 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 902 gaaggtcgga gtcaacggat ttcgaaaaat tgaagagttc cggg 44 <210> 903 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 903 tggatagctt cccttctctc gt 22 <210> 904 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 904 gaaggtgacc aagttcatgc ttcctttaga atccaccaat cttctg 46 <210> 905 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 905 gaaggtcgga gtcaacggat ttcctttaga atccaccaat cttcta 46 <210> 906 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 906 accaaaggaa gctgagaaat gg 22 <210> 907 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 907 gaaggtgacc aagttcatgc ttcaaattga agaacacctc cactt 45 <210> 908 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 908 gaaggtcgga gtcaacggat ttcaaattga agaacacctc cactg 45 <210> 909 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 909 tcccaatcca cttaaggtta cca 23 <210> 910 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 910 gaaggtgacc aagttcatgc tagccatgaa gttctttttg agtgg 45 <210> 911 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 911 gaaggtcgga gtcaacggat tagccatgaa gttctttttg agtgt 45 <210> 912 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 912 ccaaggcatt aacacatcgc aa 22 <210> 913 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 913 gaaggtgacc aagttcatgc tgaatgctca gaggatagga aacg 44 <210> 914 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 914 gaaggtcgga gtcaacggat tgaatgctca gaggatagga aaca 44 <210> 915 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 915 tgaaccagga aatcaggttg t 21 <210> 916 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 916 gaaggtgacc aagttcatgc tacgattctt tgatttatag gagggc 46 <210> 917 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 917 gaaggtcgga gtcaacggat tacgattctt tgatttatag gagggt 46 <210> 918 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 918 agttttccga ctttatggag aaacc 25 <210> 919 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 919 gaaggtgacc aagttcatgc tgagttgcat tggcttacac taaatc 46 <210> 920 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 920 gaaggtcgga gtcaacggat tgagttgcat tggcttacac taaatt 46 <210> 921 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 921 atttgacttt gtttttaaac cacgt 25 <210> 922 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 922 gaaggtgacc aagttcatgc ttgctaaaat aagtgagtgg cttgt 45 <210> 923 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 923 gaaggtcgga gtcaacggat ttgctaaaat aagtgagtgg cttgc 45 <210> 924 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 924 actagaaaaa catgccctcg a 21 <210> 925 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 925 gaaggtgacc aagttcatgc tagcttgccg gtgtgggtt 39 <210> 926 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 926 gaaggtcgga gtcaacggat tagcttgccg gtgtgggtc 39 <210> 927 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 927 tgttgttcat tcggtgcttt gc 22 <210> 928 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 928 gaaggtgacc aagttcatgc tctggagccc aataatttga gtga 44 <210> 929 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 929 gaaggtcgga gtcaacggat tctggagccc aataatttga gtgg 44 <210> 930 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 930 acttgggact cttgacagca 20 <210> 931 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 931 gaaggtgacc aagttcatgc tacgttaagc tctatctctt gttct 45 <210> 932 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 932 gaaggtcgga gtcaacggat tacgttaagc tctatctctt gttcc 45 <210> 933 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 933 catccttagc acccttgtcc t 21 <210> 934 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 934 gaaggtgacc aagttcatgc tttggttgct cgacataatt gttt 44 <210> 935 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 935 gaaggtcgga gtcaacggat tttggttgct cgacataatt gttc 44 <210> 936 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 936 tgcaaaacct aatctccggc t 21 <210> 937 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 937 gaaggtgacc aagttcatgc tcggactatc atggcttgtt gtt 43 <210> 938 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 938 gaaggtcgga gtcaacggat tcggactatc atggcttgtt gtc 43 <210> 939 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 939 cgagtcacca tcgacttcct 20 <210> 940 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 940 gaaggtgacc aagttcatgc ttgcctcatg gactgaagtt tttg 44 <210> 941 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 941 gaaggtcgga gtcaacggat ttgcctcatg gactgaagtt ttta 44 <210> 942 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 942 gtttcaacca ctaccaatcg ct 22 <210> 943 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 943 gaaggtgacc aagttcatgc tcgacgaact tcggttacaa agac 44 <210> 944 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 944 gaaggtcgga gtcaacggat tcgacgaact tcggttacaa agat 44 <210> 945 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 945 atgttgttga gacatgcgcc 20 <210> 946 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 946 gaaggtgacc aagttcatgc tgggggaact gtggtgcaac 40 <210> 947 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 947 gaaggtcgga gtcaacggat tgggggaact gtggtgcaat 40 <210> 948 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 948 tttccagtgg tctctgacag c 21 <210> 949 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 949 gaaggtgacc aagttcatgc tggctcatgg ataaaagtcg aacag 45 <210> 950 <211> 45 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 950 gaaggtcgga gtcaacggat tggctcatgg ataaaagtcg aacaa 45 <210> 951 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 951 ggtgtgcctg attttgccat 20 <210> 952 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 952 gaaggtgacc aagttcatgc tcctcccgat gcataaaact gtt 43 <210> 953 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 953 gaaggtcgga gtcaacggat tcctcccgat gcataaaact gtc 43 <210> 954 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 954 ggcgatttgt gcgtcaagag 20 <210> 955 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 955 gaaggtgacc aagttcatgc tcggtctctc tcttaactct cttgtc 46 <210> 956 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 956 gaaggtcgga gtcaacggat tcggtctctc tcttaactct cttgtt 46 <210> 957 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 957 atcttcgtta tgcgtcgaaa gt 22 <210> 958 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 958 gaaggtgacc aagttcatgc taaggatagg aataagccct caag 44 <210> 959 <211> 44 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 959 gaaggtcgga gtcaacggat taaggatagg aataagccct caaa 44 <210> 960 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 960 ttgacgcatc aagattccat aaaat 25

Claims (6)

서열번호 1 내지 960으로 표시된 올리고뉴클레오티드에서 서열번호 1부터 시작하여 인접한 3개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 320개의 올리고뉴클레오티드 프라이머 세트를 포함하는, 마커이용여교잡(marker assisted backcross) 육종을 위한 참외(Cucumis melo var. makuwa)의 유전적 배경 구별용 KASP(kompetiive allele specific PCR)용 프라이머 세트 조성물.Marker assisted backcross, including a set of 320 oligonucleotide primers, characterized in that three adjacent oligonucleotides are one primer set starting from SEQ ID NO: 1 in the oligonucleotides represented by SEQ ID NOs: 1 to 960 melon for breeding primers for genetic background distinguish KASP (kompetiive allele specific PCR) for the (Cucumis melo var. makuwa) composition. 삭제delete 제1항에 따른 프라이머 세트 조성물; 및 증폭 반응을 수행하기 위한 시약을 포함하는, 마커이용여교잡(marker assisted backcross) 육종을 위한 참외의 유전적 배경 구별용 키트.The primer set composition according to claim 1; And a reagent for carrying out an amplification reaction, a kit for distinguishing the genetic background of melon for marker assisted backcross breeding. 제3항에 있어서, 상기 증폭 반응을 수행하기 위한 시약은 DNA 폴리머라제, dNTPs 및 버퍼를 포함하는 것을 특징으로 하는 키트.The kit according to claim 3, wherein the reagent for performing the amplification reaction comprises DNA polymerase, dNTPs, and a buffer. 참외 시료에서 게놈 DNA를 분리하는 단계;
상기 분리된 게놈 DNA를 주형으로 하고, 제1항에 따른 프라이머 세트 조성물을 이용하여 KASP(kompetitive allele specific PCR)를 수행하는 단계; 및
상기 KASP의 증폭산물을 분석하는 단계;를 포함하는, 마커이용여교잡(marker assisted backcross) 육종을 위한 참외의 유전적 배경을 구별하는 방법.Separating genomic DNA from the melon sample;
Using the isolated genomic DNA as a template, and performing a kompetitive allele specific PCR (KASP) using the primer set composition according to claim 1; And
Analyzing the amplification product of the KASP; comprising, a method for distinguishing the genetic background of melon for marker assisted backcross breeding. 제5항에 있어서, 상기 증폭 산물의 검출은 DNA 칩, 방사성 측정, 형광 측정 또는 인광 측정을 통해 수행되는 것을 특징으로 하는 방법.The method of claim 5, wherein the detection of the amplification product is performed through a DNA chip, radioactivity measurement, fluorescence measurement, or phosphorescence measurement.
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