KR102231977B1 - 핵산 서열의 결정 방법 - Google Patents

핵산 서열의 결정 방법 Download PDF

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KR102231977B1
KR102231977B1 KR1020187034503A KR20187034503A KR102231977B1 KR 102231977 B1 KR102231977 B1 KR 102231977B1 KR 1020187034503 A KR1020187034503 A KR 1020187034503A KR 20187034503 A KR20187034503 A KR 20187034503A KR 102231977 B1 KR102231977 B1 KR 102231977B1
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South Korea
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nucleotide
nucleic acid
polymerase
dna polymerase
optionally
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KR1020187034503A
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English (en)
Korean (ko)
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KR20190003660A (ko
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칸다스와미 비자얀
피나르 이위도간
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옴니옴 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020187034503A 2016-04-29 2017-04-28 핵산 서열의 결정 방법 KR102231977B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662329489P 2016-04-29 2016-04-29
US62/329,489 2016-04-29
US201762444733P 2017-01-10 2017-01-10
US62/444,733 2017-01-10
PCT/US2017/030135 WO2017190012A1 (en) 2016-04-29 2017-04-28 Method of nucleic acid sequence determination

Publications (2)

Publication Number Publication Date
KR20190003660A KR20190003660A (ko) 2019-01-09
KR102231977B1 true KR102231977B1 (ko) 2021-03-24

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Application Number Title Priority Date Filing Date
KR1020187034503A KR102231977B1 (ko) 2016-04-29 2017-04-28 핵산 서열의 결정 방법

Country Status (9)

Country Link
US (2) US10584379B2 (ja)
EP (1) EP3449011B1 (ja)
JP (1) JP6915939B2 (ja)
KR (1) KR102231977B1 (ja)
CN (1) CN109154024B (ja)
AU (1) AU2017258523B2 (ja)
CA (1) CA3022431C (ja)
SG (1) SG11201809522WA (ja)
WO (1) WO2017190012A1 (ja)

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EP3986907A1 (en) * 2019-06-21 2022-04-27 Thermo Fisher Scientific Baltics UAB Oligonucleotide-tethered triphosphate nucleotides useful for labelling nucleic acids for preparing next-generation sequencing libraries
US11180520B2 (en) 2019-09-10 2021-11-23 Omniome, Inc. Reversible modifications of nucleotides
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
EP4045683A1 (en) 2019-10-18 2022-08-24 Omniome, Inc. Methods and compositions for capping nucleic acids
EP4055155A1 (en) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Engineered polymerases for improved sequencing by binding
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
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WO2021225886A1 (en) 2020-05-05 2021-11-11 Omniome, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
WO2022006081A1 (en) * 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US20220356515A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
US20220356519A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
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Publication number Publication date
US20200199668A1 (en) 2020-06-25
AU2017258523A1 (en) 2018-11-22
CN109154024B (zh) 2022-03-04
US11976323B2 (en) 2024-05-07
US20170314072A1 (en) 2017-11-02
CA3022431C (en) 2023-03-21
US10584379B2 (en) 2020-03-10
JP6915939B2 (ja) 2021-08-11
EP3449011B1 (en) 2020-11-04
CA3022431A1 (en) 2017-11-02
KR20190003660A (ko) 2019-01-09
AU2017258523B2 (en) 2020-08-13
JP2019526225A (ja) 2019-09-19
CN109154024A (zh) 2019-01-04
EP3449011A1 (en) 2019-03-06
SG11201809522WA (en) 2018-11-29
WO2017190012A1 (en) 2017-11-02

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