KR102199675B1 - 해삼정자 초저온 냉동 보존방법 - Google Patents

해삼정자 초저온 냉동 보존방법 Download PDF

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Publication number
KR102199675B1
KR102199675B1 KR1020207032341A KR20207032341A KR102199675B1 KR 102199675 B1 KR102199675 B1 KR 102199675B1 KR 1020207032341 A KR1020207032341 A KR 1020207032341A KR 20207032341 A KR20207032341 A KR 20207032341A KR 102199675 B1 KR102199675 B1 KR 102199675B1
Authority
KR
South Korea
Prior art keywords
cryopreservation
sea cucumber
liquid nitrogen
sperm
freezing tube
Prior art date
Application number
KR1020207032341A
Other languages
English (en)
Korean (ko)
Inventor
야칭 창
야오야오 잔
탄쥔 자오
지엔 송
웨이지에 장
Original Assignee
따리엔 오션 유니버시티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201910653260.1A external-priority patent/CN110326610B/zh
Application filed by 따리엔 오션 유니버시티 filed Critical 따리엔 오션 유니버시티
Application granted granted Critical
Publication of KR102199675B1 publication Critical patent/KR102199675B1/ko

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0601—Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020207032341A 2019-07-19 2020-05-19 해삼정자 초저온 냉동 보존방법 KR102199675B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201910653260.1 2019-07-19
CN201910653260.1A CN110326610B (zh) 2019-07-19 2019-07-19 海参精子超低温冷冻保存方法
PCT/CN2020/090969 WO2021012763A1 (zh) 2019-07-19 2020-05-19 海参精子超低温冷冻保存方法

Publications (1)

Publication Number Publication Date
KR102199675B1 true KR102199675B1 (ko) 2021-01-08

Family

ID=74130482

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020207032341A KR102199675B1 (ko) 2019-07-19 2020-05-19 해삼정자 초저온 냉동 보존방법

Country Status (2)

Country Link
JP (1) JP6983452B2 (ja)
KR (1) KR102199675B1 (ja)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116034992B (zh) * 2023-03-08 2023-06-27 中国科学院海洋研究所 刺参精子低温保存液及应用和刺参精子的保存方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Jacqueline Tokarew, "Investigating the Relationship Between Structure, Ice Recrystallization Inhibition Activity and Cryopreservation Ability of Various Galactopyranose Derivatives ", University of Ot *
Li Yu 외 4명, "Effects of environment factors on initiation of sperm motility in sea cucumber Apostichopus japonicus (Selenka)", J. Ocean Univ. China, Vol. 10, No. 2, pp. 165-169, 2011.04.28. *
Ming Yu Shao 외 4명, "Cryopreservation of sea cucumber Apostichopus japonicus (Selenka) sperm", Aquaculture Research, Vol. 37, No. 14, pp. 1450-1457, 2006.09.08. *
Yuta Mizuno 외 3명, "Motility and fertility of cryopreserved spermatozoa of the Japanese sea cucumber Apostichopus japonicus", Aquac Res., Vol. 50, No. 1, pp. 106-115, 2018.10.25. *

Also Published As

Publication number Publication date
JP2021525790A (ja) 2021-09-27
JP6983452B2 (ja) 2021-12-17

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