KR102197910B1 - Phytoncide-containing antimicrobial and antiviral composition - Google Patents

Abstract

Provided is a phytoncide-containing bactericidal and virucidal composition with excellent human safety. The phytoncide-containing bactericidal and virucidal composition comprises: 30 to 50 parts by weight of a first compound having antibacterial and antiviral action; 20 to 40 parts by weight of a second compound having a bronchial strengthening action; and 10 to 30 parts by weight of a third compound. The first compound includes Chamaecyparis obtusa extract, Artemisia princeps leaf extract, Citrus medica limonum(lemon) peel oil, Cryptomeria japonica bark extract, Paulownia tomentosa leaf extract, and Pinus sylvestris cone extract. The second compound includes Shirakaba ekisu (JTN) extract, and Prunus serrulata bark extract. The third compound includes Quercus acutissima extract, Juglans regia (walnut) wood extract, Cupressus sempervirens bark extract, Diospyros kaki leaf extract, Pyrus malus (apple) stem extract, Quercus robur wood extract, Cydonia oblonga leaf extract, and Citrus junos peel extract.

Description

Phytoncide-containing antiseptic and antiviral composition {PHYTONCIDE-CONTAINING ANTIMICROBIAL AND ANTIVIRAL COMPOSITION}

The present invention relates to a bactericidal and bactericidal composition, and more particularly, to a phytoncide-containing bactericidal and virucidal composition.

With the corona 19 virus outbreak around the world, disinfectant products are used not only in general homes but also in each building for quarantine. However, there are many products that are still controversial about whether they are harmful to the human body.

In particular, it has been found that chloromethylisothiazolinone (CMIT) and methylisothiazolinone (MIT) used in humidifier disinfectants cause lung diseases.CMIT and MIT, like hypochlorous acid, are chemicals that prevent the growth of microorganisms and bacteria. It is a substance.

These ingredients have been confirmed to be safe for skin toxicity, but there is no research result on toxicity that occurs when inhaled through the respiratory tract, and there is no sanctions in place, so human safety cannot be guaranteed.

[Prior patent literature]

Korean Patent Registration No. 10-1633264 (2016.06.20)

The problem to be solved by the present invention is to provide a sterilizing and bactericidal composition containing phytoncide excellent in human safety.

The problem to be solved by the present invention is not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

Phytoncide-containing bactericidal and bactericidal compositions according to embodiments of the present invention in order to achieve the object to be solved include: 30 to 50 parts by weight of the first compound with antibacterial and antiviral action; And 20 to 40 parts by weight of the second compound for strengthening the bronchi; Including 10 to 30 parts by weight of a third compound, wherein the first compound includes a cypress extract, a mugwort extract, a lemon peel oil, a cedar extract, a true paulownia leaf extract, and a chinensis pine cone extract, and the second compound is It includes a tree extract, a cherry tree bark extract, and the third compound includes acorn extract, walnut tree extract, pine tree extract, persimmon tree extract, apple extract, lobe oak extract, quince tree extract, and citron bark extract.

According to embodiments, the first compound may further include rose oil.

According to embodiments, the second compound may further include a loquat leaf extract.

According to embodiments, the second compound may further include at least one of a green tea leaf extract and an Eoseongcho extract.

According to embodiments, the first compound may further include a pine cone extract of Guju tree.

According to embodiments, the third compound further comprises at least one selected from the group consisting of lavender oil, orange peel oil, grapefruit peel oil, rosewood oil, rosemary leaf oil, jasmine oil, and PG-20 sorbitancocoate. can do.

Details of other embodiments are included in the detailed description and drawings.

Phytoncide-containing bactericidal and bactericidal compositions according to embodiments of the present invention are made of natural materials and are not only harmless to the human body, but also have excellent antibacterial and antiviral activity, so that they can be usefully used in places with high potential for propagation of various bacteria and viruses. . In addition, since it contains broncho-strengthening ingredients, it can show excellent human safety even when inhaled with a respiratory tract.

1 is a view for explaining a method of preparing a sterilizing and bactericidal composition containing phytoncide according to an embodiment of the present invention.
Figure 2 is a photograph of a phytoncide-containing sterilizing and bactericidal composition according to an embodiment of the present invention before contact with pneumococcal 5 seconds after contact.

In order to fully understand the configuration and effects of the present invention, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various forms and various changes may be added.

In addition, terms used in the embodiments of the present invention may be interpreted as meanings commonly known to those of ordinary skill in the art, unless otherwise defined.

Phytoncide-containing sterilization and bactericidal composition according to an embodiment of the present invention may be a liquid composition for disinfecting viruses or bacteria in the air or surface viruses or bacteria of a subject.

Phytoncide-containing bactericidal and bactericidal compositions according to an embodiment of the present invention may include a first compound that performs an antibacterial and antiviral action, and a second compound and a third compound that perform a bronchial strengthening action. According to an embodiment, the bactericidal and bactericidal composition containing phytoncide may include 40 to 80 parts by weight of the first compound, 20 to 60 parts by weight of the second compound, and 10 to 30 parts by weight of the third compound.

The first compounds include chamaecyparis obtusa extract, artemisia princeps leaf extract, lemon peel oil (citrus medica limonum (lemon) peel oil), cryptomeria japonica bark extract, and paulownia leaf extract ( paulownia tomentosa leaf extract) and pinus pine cone extract (PINUS SYLVESTRIS CONE EXTRACT).

For example, the first compound is 5 to 15 parts by weight of cypress extract, 5 to 15 parts by weight of mugwort extract, 5 to 15 parts by weight of lemon peel oil, 5 to 15 parts by weight of cedar extract, 1 to 5 parts by weight of paulownia leaf extract And it may contain 5 to 15 parts by weight of the pine cone extract of Guju tree.

Cypress extract has antibacterial effect, antiviral effect, rhinitis, asthma prevention effect, allergy and atopy relief effect, and stress relief effect. Mugwort extract is effective in antiviral, antibacterial, and bleeding, menstrual irregularities, fetal anxiety, and infertility. Lemon peel oil has antibacterial properties, helps reduce heat, improves concentration and memory, stabilizes emotions, improves mood, and helps regenerate skin. It is also effective against acne skin, spots, and freckles. Cedar extract has antibacterial, antiviral, anti-inflammatory, and moisturizing effects. Paulownia leaf extract has antiviral effect, antibacterial effect, bronchitis treatment effect, detoxification effect, expectorant effect, etc., and makes hair shiny. In addition, the pine cone extract of Gujumi tree has antibacterial and antiviral effects.

According to an embodiment, the first compound may further include rose flower oil.

For example, the first compound is 1 to 10 parts by weight of cypress extract, 1 to 5 parts by weight of mugwort extract, 1 to 5 parts by weight of lemon peel oil, 1 to 10 parts by weight of cedar extract, 1 to 10 parts by weight of hinokitiol, cham paulownia It may contain 1 to 10 parts by weight of tree leaf extract and 1 to 10 parts by weight of rose oil.

Rose flower oil is effective in antibacterial, anti-inflammatory, toxin removal and digestion, and has skin regeneration, soothing, and moisturizing effects. The pine cone extract of Guzoia chinensis has antibacterial and antiviral effects.

The second compound may include birch extract (shirakaba ekisu (JTN)) and cherry tree bark extract (prunus serrulata bark extract).

For example, the second compound may include 1 to 13 parts by weight of birch extract and 1 to 13 parts by weight of cherry bark extract.

Birch extract is effective in diuresis, pain relief, antipyretic and detoxification, and is effective in treating tonsillitis, pneumonia, bronchitis, nephritis, urethritis, cystitis, rheumatism, gout, and skin diseases. Cherry bark extract strengthens bronchial and cardiopulmonary function, strengthens gastrointestinal function, is effective against heat rash, eczema, acne, and skin diseases, and is effective against food poisoning, fish poisoning, and mushroom poisoning.

According to an embodiment, the second compound may further include a loquat leaf extract (eriobotrya japonica leaf extract), and the second compound is among the green tea leaf extract (camellia sinensis leaf extract) and the eoseongcho extract (houttuynia cordata extract). It may further include at least one.

For example, it may include 1 to 10 parts by weight of birch extract, 1 to 10 parts by weight of cherry bark extract, 1 to 10 parts by weight of loquat leaf extract, 1 to 5 parts by weight of green tea leaf extract, and 1 to 5 parts by weight of Eoseongcho extract. have.

Loquat leaf extract is effective in lung disease, Jinhae, Geodam, Sogal, chronic bronchitis, hiccups, beriberi, vomiting, Gi (氣) lowering, liquor poison (酒毒), skin beauty, refreshing (淸凉), and geodam. In addition, green tea leaf extract is effective in removing active oxygen, soothing and moisturizing the skin, and has antioxidant and anti-aging effects. Eoseongcho extract has anti-cancer, detoxification, and suspicious stool removal effects, improves inflammatory and skin diseases, and is effective in removing waste products and eliminating toxins.

According to an embodiment of the present invention, the bactericidal and viral composition containing phytoncide may further include a third compound. In this case, the bactericidal and bactericidal composition containing phytoncide may include 40 to 60 parts by weight of the first compound, 20 to 40 parts by weight of the second compound, and 10 to 30 parts by weight of the third compound.

The third compounds are quercus acutissima extract, juglans regia (walnut) wood extract, cupressus sempervirens bark extract, quercus robur wood extract, and diospyros kaki. leaf extract), apple extract (pyrus malus (apple) stem extract), quince tree extract (cydonia oblonga leaf extract), citron peel extract (citrus junos peel extract) may be included.

As an example, the third compound includes lavender oil (lavandula angustifolia (lavender) oil), orange peel oil (citrus aurantium dulcis (orange) oil), grapefruit peel oil (citrus grandis (grapefruit) peel oil), rosewood oil (aniba rosaeodora ( rosewood) wood oil), rosemary leaf oil (rosemarinus officinalis (rosemary) leaf oil), jasmine oil (jasminum officinale (jasmine) oil), PEG-20 sorbitan cocoate (PEG-20 sorbitan cocoate) selected from the group consisting of It may further include at least one.

Hereinafter, a method of preparing a sterilizing and bactericidal composition containing phytoncide according to an embodiment of the present invention will be briefly described.

1 is a diagram illustrating a method of preparing a sterilizing and bactericidal composition containing phytoncide according to an embodiment of the present invention.

Referring to FIG. 1, raw materials such as various fruits, seeds, roots, stems, bark, leaves, flowers, and vitamins are dried. Drying can be done naturally or using a drying machine.

Extract after drying raw materials. According to an embodiment, leaves and flowers are distilled extracted, roots, stems, and bark are dry-extracted, and fruits and seeds may be extracted by pressing.

In the case of leaves and flowers, a mixture may be obtained from raw materials of leaves and flowers by primary purification using steam after distillation extraction, heating to secondary purification, and filtering.

In the case of roots, stems, and bark, the mixture may be obtained from raw materials of the roots, stems, and bark after heating and purification, followed by filtering.

In the case of fruits and seeds, a mixture can be obtained from fruits and seeds by filtering, heating and sterilizing immediately after compression extraction.

Mixtures from raw materials of leaves and flowers, mixtures from raw materials of roots, stems, and bark, and mixtures from fruits and seeds are mixed with essential oils and purified water, and filtered to obtain phytoncide-containing bactericidal and viral compositions.

Subsequently, the sterilizing and bactericidal composition containing phytoncide of the good product is filled and packaged through an inspection process.

Hereinafter, in order to investigate the sterilization and sterilization effect of the phytoncide-containing sterilization and bactericidal composition according to an embodiment of the present invention, an experimental example will be exemplified.

Experimental example

9 parts by weight of cypress extract, 9 parts by weight of rose flower oil, 9 parts by weight of Guzo tree pine cone extract, 9 parts by weight of cedar extract, 9 parts by weight of paulownia leaf extract, 9 parts by weight of birch extract, 9 parts by weight of cherry bark extract, loquat 9 parts by weight of leaf extract, 4 parts by weight of green tea leaf extract, 5 parts by weight of mugwort extract, 5 parts by weight of Eoseongcho extract, and 5 parts by weight of lemon peel oil were mixed to prepare a phytoncide-containing sterilizing and bactericidal composition.

COVID-19 virus sterilization and viral sterilization experiment

Test virus: COVID-19 (SARS-CoV-2)

Cell line: Vero-E6

Experimental results at the reaction time of 30 seconds, 1 minute, and 5 minutes are shown in [Table 1].

Processing time Virus reduction rate [log] [%] 30 seconds ≥4.00 99.99% 1 minute ≥4.00 99.99% 5 minutes ≥4.00 99.99%

Phytoncide-containing bactericidal and bactericidal composition according to an embodiment of the present invention showed 99.99% virus killing efficacy in 30 seconds treatment as a result of a bactericidal power test for COVID-19 (SARS-CoV-2).

Influenza virus sterilization and viral sterilization experiment

Test Virus: Influenza Virus

Cell line: MDCK

Experimental results at the reaction time of 30 seconds, 1 minute, and 5 minutes are shown in [Table 2].

Processing time Virus reduction rate [log] [%] 30 seconds ≥3.67 99.98% 1 minute ≥3.67 99.98% 5 minutes ≥3.67 99.98%

Phytoncide-containing bactericidal and bactericidal composition according to an embodiment of the present invention showed 99.98% virus killing efficacy in 30 seconds treatment as a result of a sterilization test for influenza virus.

Pneumococcal sterilization and viral test

Test bacteria: Klebsiella pneumoniae ATCC 4352

Badge: Nutrient broth/Nutrient agar

Preparation of test bacteria: 0.85% NaCl

Initial bacterial concentration: 2.9 X 10 ⁶ CFU/mL

Control: sterile distilled water

Contact time: 5 seconds

Temperature: 20 ±2 ℃

Other conditions: Voltex shaking

Neutralizing solution: D/E broth

The sterilization and bactericidal power were calculated by the following equation.

Sterilization power (%) [(A-B)/A] X 100 (A: initial number of bacteria, B: number of specimens after contact

2 is a photograph of a phytoncide-containing sterilizing and bactericidal composition according to an embodiment of the present invention before contact with pneumococcal bacteria 5 seconds after contact.

Referring to Figure 2, there is an effect of killing 99.9% pneumococcal bacteria after 5 seconds contact with the sterilizing and bactericidal composition containing phytoncide according to an embodiment of the present invention.

Ammonia removal experiment

Sample input: 15 mL

Test gas: Ammonia (NH ₃ )

Concentration of injected test gas: 100 μ

Test gas injection volume: 1.5L

Test container: 3L Tedler bag

Temperature is 20 ±2 ℃ Humidity is 40 ±5 %R.H.

The deodorization rate was calculated by the formula of [Table 3] below.

Deodorization rate (%)=[(blank gas concentration- sample gas concentration)/blank gas concentration] X 100

Test time Deodorant rate 30 minutes More than 99% 60 minutes More than 99% 90 minutes More than 99% 120 minutes More than 99%

When the sterilizing and bactericidal composition containing phytoncide according to an embodiment of the present invention was brought into contact with ammonia for 30 minutes, the deodorization rate was greater than 99%.

Confirmation result of household chemical products subject to safety confirmation

Chemical substance confirmation result Confirmation item unit Confirmation criteria Confirmation result Judgment Remark Formaldehyde mg/kg 40 or less Not detected fitness Content Restricted Substance (Contains) Acetaldehyde mg/kg 100 or less Not detected fitness chloroform mg/kg 30 or less Not detected fitness Content Restricted Substance (Contains) Sodium nitrite mg/kg 80 or less as nitrite ion Not detected fitness Content Restricted Substance (Contains) Persulfate compound - - Not applicable Not applicable Content Restricted Substance (Contains) Containing prohibited substances - Not contained Not contained fitness Submission of non-contained non-use confirmation

Container/packaging and weight check result Inspection classification Judgment Nonconformity Remark Containerㆍpackaging and weight fitness - Check appearance, strength and leakage, weight/capacity/weekly result (suitable) Child protection packaging fitness - Non-target

As a result of the confirmation of the household chemical products subject to safety confirmation, the sterilizing and bactericidal composition containing phytoncide according to the present invention was comprehensively determined to be suitable. [0066] The embodiments of the present invention have been described above with reference to the accompanying drawings, but the technical field to which the present invention belongs Those of ordinary skill in the art will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and are not limiting.

Claims (6)

30 to 50 parts by weight of the first compound with antibacterial and antiviral action; And
20 to 40 parts by weight of the second compound for strengthening the bronchi;
Including 10 to 30 parts by weight of the third compound,
The first compound,
It contains cypress extract, mugwort extract, lemon peel oil, cedar extract, paulownia leaf extract, rose flower oil, pine cone extract,
The second compound,
It contains birch extract, cherry bark extract, green tea leaf extract, and Eoseongcho extract,
The third compound,
Phytoncide-containing bactericidal and bactericidal composition comprising acorn extract, walnut extract, pine tree extract, persimmon tree extract, apple extract, lobe oak extract, quince tree extract, yuzu bark extract, loquat leaf extract. The method of claim 1,
The third compound is phytoncide-containing sterilization and further comprising at least one selected from the group consisting of lavender oil, orange peel oil, grapefruit peel oil, rosewood oil, rosemary leaf oil, jasmine oil, and PG-20 sorbitancocoate. Viral composition. delete delete delete delete

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