KR102161568B1 - A feed composition comprising insect breeded by chlorella - Google Patents

Abstract

The present invention relates to a composition for feed for livestock containing insects bred with a composition for feed of insects containing chlorella, in particular, in a feed composition for aquaculture comprising Dongae, etc. bred by feeding a composition for feed of insects containing chlorella, The composition for insect feed relates to a feed composition for aquaculture, characterized in that it contains 15% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed. In addition, the present invention relates to a composition for insect feed containing chlorella.
The feed composition and the insect feed composition according to the present invention are economical due to a simple feed manufacturing process, and the content of unsaturated fatty acids including omega 3 fatty acids in Dongae, which is a raw material of feed, can be improved using chlorella, so the quality is very excellent. In addition, it is possible to effectively improve the production efficiency of feed by improving the growth rate of Dongae, which is a raw material of feed, to have critical significance, and recycle materials that cause environmental pollution such as food waste in breeding in Dongae, etc. Therefore, it is environmentally friendly, and can have a remarkably excellent effect not only in the feed manufacturing related industry but also in the livestock or aquaculture industry using feed.

Description

Composition for livestock feed containing insects reared with a composition for insect feed containing chlorella {A FEED COMPOSITION COMPRISING INSECT BREEDED BY CHLORELLA}

The present invention relates to a composition for feed comprising an insect, specifically a composition for feed comprising an insect raised with an insect feed composition comprising chlorella, a composition for feed for livestock or a composition for fish.

In recent years, as the awareness that aquatic products are health foods spreads, consumption of aquatic products is increasing, and accordingly, fish farming is also increasing in quantity and quality. However, due to coastal pollution, aquaculture efficiency, and diseases of aquaculture organisms, the supply of fishery products through fish farming has not increased significantly. The reason for this can be found in the inability to actively develop aquaculture fisheries, but fundamentally, the reason can be found in the low efficiency of aquaculture of fish.

Accordingly, there is a need for development of aquaculture feed that not only improves the growth efficiency and feed efficiency of fish, but also improves the meat quality or quality of aquaculture fish.

On the other hand, insect feeds manufactured using insects worldwide are being used as feed additives of less than 5%, and their use is limited. In particular, larvae in Dongae et al. are mentioned in the Food Agriculture Organization (FAO)'s Future Food Report as an insect to be substituted as the main source of feed for future livestock, despite being pointed out as an insect feed that can be used for feed. Has a limiting aspect.

Dongae et al. is an insect belonging to the family Arthropod Insect River Flies Order Dongae et al., and its body is black, its body length is 13 to 20 mm, and lives in dirty water or manure, and is globally used in the United States, India, Australia, Vietnam, and Korea. Are distributed as. In general, in Dongae and the like, animal carcasses, livestock meals, plant residues, and food waste, which are organic wastes, are used as food, and food waste is used as food.

In the Dongae et al., the period of growth from eggs to adults is 37 to 41 days, and the period of larvae acting as a decomposer is about 14 days. The larvae of the Dongae et al. have a habit of crawling out to find a dry place to become a pupa after a period (about 14 days) in which organic waste can be decomposed. For example, if 5000 larvae are added to Dongae etc. to 10kg of food waste, more than 80% of the food waste is decomposed in 5 days, and the decomposed food waste is reduced by about 42% in volume and about 70% in weight compared to before the larvae are added. .

Therefore, in the case of manufacturing feed using Dongae, etc., it is evaluated that it can contribute to an eco-friendly and sustainable aquaculture environment or livestock environment by reusing larvae in the feed, such as Dongae, which are used for food waste treatment and animal manure treatment. To this end, the development of feed using Dongae et al., which is supplemented with nutrients, is required. In particular, larvae in Dongae et al. have a significantly low omega-3 fatty acid component such as EPA, which has an important effect on the growth rate and survival rate of fry, and there is a problem that is unsuitable for use as aquaculture feed for aquaculture fish. Problems such as an increase in production cost and environmental pollution of the used aquaculture feed have emerged, and the need for research to overcome this has increased.

The above-described background technology is technical information possessed by the inventors for derivation of the present invention or acquired during the derivation process of the present invention, and is not necessarily known to be publicly known prior to filing the present invention.

10-0937152B 10-1076686B 10-1156061B 10-1838201B 1020180051459A

An object of the present invention is to solve the problems of the prior art as described above, and the present invention is to provide a feed composition comprising insects reared with an insect feed composition that is more economical and has excellent feed quality.

In addition, in order to solve the problems of the prior art as described above, the present invention provides a composition for feeding insects for breeding insects, which are raw materials of the feed composition.

In addition, in order to solve the problems of the prior art as described above, the present invention provides an insect reared with the insect feed composition of the present invention.

In order to achieve the above object, the present invention provides a feed composition. The feed composition may be a composition for livestock feed or a composition for aquaculture feed.

In addition, the present invention provides a composition for insect feed in order to achieve the above object. The composition for insect feed may be a composition for insect feed for breeding in Dongae and the like.

In addition, the present invention provides an insect reared with the composition for insect feed in order to achieve the above object. The insect may be Dongae et al.

The feed composition according to an embodiment of the present invention may include an insect reared with an insect feed composition containing chlorella as an active ingredient.

The feed composition may be a composition for livestock feed or a composition for aquaculture feed.

The composition for insect feed may contain 14 to 16% by weight (w/w), preferably 15% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed.

The feed composition, characterized in that the insects bred with the composition for insect feed are Dongae et al.

Preferably, the feed composition of the present invention is a feed for aquaculture, including Dongae, which was bred by feeding a composition for insect feed containing chlorella containing 15% by weight (w/w) of chlorella based on the total weight of the composition for insect feed. It may be a composition.

In this aspect, the present invention relates to a feed composition for aquaculture comprising Dongae, etc. bred by feeding a composition for insect feed containing chlorella, the composition for insect feed, based on the total weight of the composition for insect feed, chlorella is 5 weight It may be a feed composition for aquaculture, which is characterized in that it contains% (w/w).

In another aspect, the composition for insect feed according to another embodiment of the present invention may include chlorella.

The composition for insect feed may contain 14 to 16% by weight (w/w), preferably 15% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed.

The composition for insect feed may be a composition for insect feed for breeding in Dongae, etc.

In another aspect, the Dongae deungae according to another embodiment of the present invention may be bred with the composition for insect feed of the present invention, specifically, the composition for insect feed containing chlorella as an active ingredient.

Dongae, etc. bred with the composition for insect feed of the present invention have an EPA (Eicosapentaenoic acid) content of 0.05% (w/w) and a linoleic acid content of 4.65% (w/w) based on the total fatty acid weight. , The content of lauric acid is 17.95% (w/w), the content of oleic acid is 4.94% (w/w), and the content of myristic acid is 2.68% (w/w) It can be.

Singular expressions used in the present specification include plural expressions unless the context clearly indicates otherwise.

In the present specification, unless otherwise specified, terms such as include or have are intended to designate the existence of features, numbers, steps, or a combination of them described in the specification, and one or more other features, numbers, steps, or It is to be understood that the presence or addition of any combination does not preclude the possibility.

Unless otherwise specified in the specification, the active ingredient refers to an ingredient provided to impart a specific effect in a composition, specifically, a composition for feed.

Unless otherwise specified in the specification, an insect refers to one belonging to the class of arthropod insects. Specifically, the insect may be meant to include a dongae, etc., a flower radish, a long-lived beetle, a brown beetle, a pair of crickets, an American king-geri, and the like, preferably in a dongae, etc., or a long-lived beetle, more preferably However, it is not limited thereto.

Unless otherwise specified in the present specification, Dongae et al.means an insect belonging to the Arthropod Insect River Flies (Diptera) Dongae Deunge (Stratomyidae), and specifically American Dongae et al.(Black Soldier Fly, Hermetia illucens ) may be.

Larvae of flies such as Dongae et al. are known to be good sources of essential amino acids such as arginine, methionine, and lysine, and these amino acids are generally higher than soybean meal and at the same level as fish meal. Thus, fly larvae have sufficient potential as a protein source for livestock feed or aquaculture feed. In particular, Dongae et al. larvae are mentioned in the Food Agriculture Organization (FAO)'s Future Food Report as an insect that will be a major source of feed for future livestock, and are attracting attention as insect feed that can be converted into feed. However, larvae of Dongae et al. have significantly low omega-3 fatty acid components such as EPA, which have an important effect on the growth rate and survival rate of fry, so it is required to overcome these problems in order to be used as aquaculture feed for aquaculture fish.

In the Dongae et al., the period of growth from eggs to adults is 37 to 41 days, and the period of larvae acting as a decomposer is about 14 days. The larvae of the Dongae et al. have a habit of crawling out to find a dry place to become a pupa after a period (about 14 days) in which organic waste can be decomposed. The Dongae et al. boast excellent dietary properties and have good reproductive ability, so it is highly likely to be used continuously.

Unless otherwise specified in the specification, Chlorella is a single-celled organism belonging to green algae growing in freshwater or seawater, and is a kind of plankton. It was studied as a food for astronauts and became famous. The algae is a common name for a lower class flower plant, which means that most of them grow in water or in a watery place, and live by themselves by assimilation with chlorophyll.

The chlorella is widely distributed on the earth from tropical to cold, and can be collected from lakes, ponds, puddles, and seawater. Chlorella is a single-celled plant, one individual is formed by one cell, and the shape is round or oval. It can only be seen under a microscope with a size of 2/10 to 10 mm, and it proliferates with a surprising degree of vitality. In Korea's Health Functional Food Code, chlorella products and spirulina products are listed as algae foods as health functional foods.

Unless otherwise specified in the specification, livestock refers to any breeding animal and may be broilers, laying hens, breeders, pigs, ducks, or fish farms, and more specifically, may include poultry, pig farms, ducks or fish farms, It is not limited thereto.

Unless otherwise specified in the specification, feed means a substance that supplies organic or inorganic nutrients necessary for maintaining the life of an individual and raising the individual. The feed may contain nutrients such as energy, protein, lipids, vitamins, minerals, etc. that the individual who consumes the feed needs, but is not particularly limited thereto, and the individual consumes the feed of the present invention to mean a breeding target. It is included without limitation as long as it is capable of living organisms, and especially includes insects and livestock for the purposes of the present invention.

The inventors of the present invention are researching various materials for the development of feed compositions, and can be easily reared using food waste, and not only remove environmental pollutants during the breeding process of insects, but also have a wealth of reared insects. Due to the protein component, Dongae et al. is selected as an insect that can be used as a raw material for feed, and in relation to fat, which is an additional nutrient ingredient in addition to the protein, EPA (Eicosapentaenoic acid), linoleic acid, oleic acid, lauric acid While researching a method to improve the growth rate of Dongae, etc., while increasing the content of omega-3 fatty acids such as EPA, which is essential for growth and survival of fry, especially unsaturated fatty acids such as myristic acid, Dongae In the case of rearing the back, if chlorella is contained in 15% (w/w) relative to the total weight of the feed composition, not only the growth amount to Dongae, etc. is most improved, but also the content of unsaturated fatty acids in the reared Dongae, especially, omega 3 fatty acids. It was confirmed that the EPA content was significantly improved, and the present invention was completed.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a feed composition comprising, as an active ingredient, insects reared with a composition for insect feed containing chlorella. The feed composition may be a composition for livestock feed or a composition for aquaculture feed.

? The composition for feed may further include the fecal soil of the insect.

The composition for livestock feed is 1 to 20% by weight (w/w), preferably 5 to 10% by weight (w/w), more preferably, of the insect on a non-dry basis, based on the total weight of the composition for livestock feed. 7.5% by weight (w/w) may be included. When the content range of the feed is satisfied, it is excellent nutritionally and has an economical effect in terms of cost.

The insect included in the feed composition may be in a non-dried state, a dry state, or a pulverized form, and may be included in a non-dried state, but is not limited thereto.

The composition for livestock feed, based on the total weight of the composition for livestock feed, 30 to 49% by weight (w/w), preferably 40 to 45% by weight (w/w), more preferably It may include 42.5% by weight (w/w).

The livestock may be one or more selected from the group consisting of broilers, laying hens, breeders, pigs, ducks, and fish farms, preferably poultry, pigs, ducks, or fish farming, more preferably fish farming, but limited thereto. It does not become.

The composition for insect feed may include 14 to 16% by weight (w/w), preferably 14% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed.

In addition, the composition for insect feed may further include cereals, watermelons, persimmons, animal oils and fats, calcium salts, salts, ginseng salts, other oils and fats, vitamins, niacin, pantothenic acid, hydrochloric acid, choline chloride, biotin, or amino acids. .

In addition, the composition for feed for insects may further include crude protein, crude fat, crude fiber, crude powder, calcium or phosphorus. The insect feed composition may have a crude protein content of 20.0% by weight (w/w) or more, a crude fat content of 3.0% by weight (w/w) or more, and a crude fiber content of 6.0 weight based on the total weight of the insect feed composition. % (w/w) or less, the crude powder content may be 8.0 wt% (w/w) or less, the calcium content may be 0.8 wt% (w/w) or more, and the phosphorus content may be 1.0 wt% (w/ w) may be less than.

In addition, the composition for insect feed may further contain one or more components selected from the group consisting of brewer's meal, bean curd, corn, barley and wheat. The insect feed composition may be based on the total weight of the insect feed composition, the brewer's meal content may be 10 to 50% by weight (w/w, based on a non-dried state), and the bean fat content is 10 to 50% by weight (w/ w, based on a non-dried state), the corn content may be 10 to 50% by weight (w/w, based on a non-dried state), and the barley content may be 10 to 50% by weight (w/w, non-dried State), and the wheat content may be 10 to 50% by weight (w/w, based on a non-dried state).

In addition, the composition for insect feed may further contain a fermenting agent.

Insects bred with the composition for insect feed may be Dongae et al., flower radish, longevity beetle, brown bite, twin cricket or American king bite, and preferably may be Dongae et al. or longevity beetle, more preferably Dongae et al. I can.

The Dongae and the like may preferably be American Dongae and the like (Black Soldier Fly, Hermetia illucens ). The American Dongae and the like can be efficiently mass-produced with a life cycle of about 40 days, and have the advantage of feeding activities only during the larvae.

The Dongae and the like may be eggs, larvae, pupae or adult worms of Dongae, etc., and specifically may be larvae or pupae, but preferably larvae. The larvae of Dongae et al. are environmental cleansing insects that live by feeding on organic waste such as animal carcasses, livestock manure, plant residues, food waste, etc., using strong decomposition ability, and after the larvae in the life cycle of Dongae et al. Since the larvae period is the most nutritionally excellent period due to inactivity, it is preferable that the larvae be the larvae in the Dongae and the like.

The feed composition is preferably raised by feeding an insect feed composition containing 14 to 16% by weight (w/w), preferably 15% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed It may be a feed composition for aquaculture comprising a Dongae et al. as an active ingredient.

In this aspect, the present invention relates to a feed composition for aquaculture comprising as an active ingredient Dongae, etc. bred by feeding a composition for insect feed containing chlorella, the composition for insect feed based on the total weight of the composition for insect feed, chlorella It may be a feed composition for aquaculture, characterized in that it contains 14 to 16% by weight (w / w).

In addition, the present invention may be a composition for insect feed containing chlorella as an active ingredient.

The composition for insect feed may contain 14 to 16% by weight (w/w), preferably 15% by weight (w/w) of chlorella, based on the total weight of the composition for insect feed.

The chlorella may be seawater, that is, chlorella collected from the sea. When the chlorella is added as a feed, it is possible to improve the content of unsaturated fatty acids, which are insufficient ingredients as feed ingredients in Dongae et al., particularly omega 3 fatty acids such as EPA, which have an important effect on the growth rate and survival rate of fry.

In addition, the composition for insect feed may further include cereals, watermelons, persimmons, animal oils and fats, calcium salts, salts, ginseng salts, other oils and fats, vitamins, niacin, pantothenic acid, hydrochloric acid, choline chloride, biotin, or amino acids. .

In addition, the composition for feed for insects may further include crude protein, crude fat, crude fiber, crude powder, calcium or phosphorus. The insect feed composition may have a crude protein content of 20.0 parts by weight or more, a crude fat content of 3.0 parts by weight or more, a crude fiber content of 6.0 parts by weight or less, and a crude content of 8.0 based on 100 parts by weight of the insect feed composition. It may be less than or equal to parts by weight, the calcium content may be 0.8 parts by weight or more, and the phosphorus content may be 1.0 parts by weight or less.

In addition, the composition for insect feed may further contain one or more components selected from the group consisting of brewer's meal, bean curd, corn, barley and wheat. The insect feed composition may be based on the total weight of the insect feed composition, the brewer's meal content may be 10 to 50 parts by weight (w/w, based on a non-dried state), and the bean fat content may be 10 to 50 parts by weight ( w/w, based on a non-dried state), the corn content may be 10 to 50 parts by weight (w/w, based on a non-dried state), and the barley content may be 10 to 50 parts by weight (w/ w, based on a non-dried state), and the wheat content may be 10 to 50 parts by weight (w/w, based on a non-dried state).

In addition, the composition for insect feed may further contain a fermenting agent.

The composition for insect feed may be for breeding dongae, etc., flower radish, longevity beetle, brown gooseneck, twin-star cricket or American king gooseneck, and preferably, may be a composition for breeding insect feed in Dongae.

The Dongae and the like may be eggs, larvae, pupae or adult worms of Dongae, etc., and specifically may be larvae or pupae, but preferably larvae.

The composition for insect feed may be used individually, or may be used in combination with a conventionally known feed additive or feed.

In addition, the present invention may be an insect bred with the composition for insect feed.

The composition for insect feed may include chlorella as an active ingredient. The chlorella may contain 14 to 16% by weight (w/w), preferably 15% by weight (w/w) of chlorella based on the total weight of the insect feed composition. Insects reared with the composition for insect feed containing the chlorella have the effect of significantly increasing the content of unsaturated fatty acids compared to insects reared with conventional feed.

The insects may be Dongae et al., flower radish, longevity beetle, brown bite, twin star cricket, or American king geese, and preferably may be Dongae et al.

In this aspect, Dongae, etc. bred with the insect feed composition have an EPA (Eicosapentaenoic acid) content of 0.05% (w/w) and a linoleic acid content of 4.65% (w/w) based on the total fatty acid weight. And, the content of lauric acid is 17.95% (w/w), the content of oleic acid is 4.94% (w/w), and the content of myristic acid is 2.68% (w/w) ) May be.

The composition for feed according to the present invention and the composition for insect feed including chlorella contain various nutrients as feed, and by reusing larvae in feed, such as Dongae, which are used for food waste treatment and animal manure treatment, eco-friendly and sustainable aquaculture environment or There is an effect that can contribute to the livestock environment. In addition, the insects bred with chlorella have an increased content of unsaturated fatty acids including omega 3 fatty acids, specifically EPA (Eicosapentaenoic acid), linoleic acid, oleic acid, palmitic acid, etc. , It can be a raw material for nutritionally excellent feed, and in particular, it can improve its value as a feed for fish farming by improving the content of EPA, which is a component insufficient in Dongae, etc., as a result, using the composition for insect feed according to the present invention. There is an advantage in that the quality of the composition for feed including reared insects can also be very excellent.

In this respect, the composition of the present invention can be widely used in various industrial fields to which feed is applied, including the aquaculture industry and the livestock industry.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

All experimental results were repeated at least three times and expressed as an average value, and the comparison with each group was determined to be significant when p <0.05, respectively, using the ANOVA test.

Manufacturing example . In Dongae Preparation of larvae and feed

The larvae of Dongae et al. used in this study were provided with larvae on the 4th to 5th day after hatching from the E company, a specialized breeding company in Dongae et al., and were reared in a breeding machine dedicated to larvae at 28 ± 1℃. Three days before the experiment, chick feed (Hanil Feed Co., Ltd., Gyeonggi-do, Korea) was adapted by supplying about three times the weight of the larva, and during the test period, the content of chlorella (Korea Bioengineering Co., Ltd. It was added differently to provide 3 times the weight of the larva.

The content of chlorella is 11 groups (control (0%), 0.1% (w/w), 0.5% (w/w), 1% (w/w), 2%) according to the content of chlorella relative to the total feed weight. (w/w), 3% (w/w), 5% (w/w), 7% (w/w), 10% (w/w), 15% (w/w) and 20% (w After dividing by /w)), it was mixed with chick feed and supplied three times the weight of the larva once a day for 7 days. After 7 days, the weight of the larva was measured, and the fatty acid content in the larva was measured.

Experimental Example 1 . According to chlorella content In Dongae Caterpillar feed efficiency

In order to check the feed efficiency of the insect feed containing chlorella at each content prepared in the above preparation example, the weight change of the larvae before and after the addition of the feed containing chlorella was measured on an electronic scale (OHAUS PAG213, OHAUS Co. , USA), and it was confirmed that the content has a high growth rate, and the results are shown in Table 1 below.

Chlorella
content(%) Weight(g) Day 0 Day 7 0 8.0±0.0 ^a 24.6±2.1 ^ab 0.1 8.0±0.0 ^a 22.4±1.0 ^a 0.5 8.0±0.0 ^a 22.3±0.9 ^a One 8.0±0.0 ^a 23.1±0.6 ^a 2 8.0±0.0 ^a 22.5±0.4 ^a 3 8.0±0.0 ^a 23.6±0.2 ^a 5 8.0±0.0 ^a 23.9±0.2 ^a 7 8.0±0.0 ^a 25.9±1.2 ^abc 10 8.0±0.0 ^a 25.8±1.1 ^abc 15 8.0±0.0 ^a 27.9±1.0 ^c 20 8.0±0.0 ^a 27.1±0.5 ^bc

As shown in Table 1, the weight of larvae increased by about 207.5 ± 26.1% in Dongae, etc., which were fed only chick feed for 7 days, but chlorella 7% (w/w) or more among the groups in which chlorella was added to the existing insect feed The weight of the larvae to the supplied Dongae et al. showed a significant increase compared to this, and the chlorella content optimal for the growth of the larvae was 15% (w/w), increasing the most to 249.2 ± 12.4% in the 15% containing group. Confirmed.

Experimental Example 2 . According to chlorella content In Dongae Fatty acid improvement efficiency in larvae

Fatty acid changes of larvae in Dongae et al. according to the addition of chlorella were confirmed by the following method.

First, fat extraction and fatty acid analysis for fatty acid analysis followed the experimental method of Food Code (2.1.5.4 fatty acid). Specifically, after washing the larvae in each Dongae etc bred in Preparation Example, freezing for 24 hours in a -35°C freezer, hot air drying for about 2 days, pulverization, and then put in a Mazonnier tube and about 100 mg After the addition of pyrogallol, 2 mL of an internal standard solution was added. The boiled side was put in a mazonier tube, 2 mL ethanol was added, and the whole sample was mixed until well mixed. After 10 mL of 8.3 M hydrochloric acid solution was added and mixed, the cap of the mazonier tube was sealed and stirred in a water bath at 70° C. for about 40 minutes. After stirring, the mixture was cooled to room temperature, and ethanol was added to the lower part of the mazonier tube to facilitate separation during ether extraction, followed by mixing gently.

20 mL diethyl ether was added to the decomposition product of the mazonier tube, and the mixture was capped and then shaken for 5 minutes to extract. The stopper was washed with an ether mixed extraction solvent, 25 mL of anhydrous petroleum ether was added, extracted with shaking for 5 minutes, and centrifuged for 5 minutes at 600 rpm. After washing the stopper with an ether mixed extraction solvent, separating the ether layer in a beaker, and slowly evaporating the ether in a 35 to 40°C water bath using nitrogen to evaporate.

Dissolve the fat extracted with 2 to 3 mL of chloroform and 2 to 3 mL of diethyl ether in the sample from which the ether was evaporated, and transfer it to a 15 mL test tube, then concentrate with nitrogen in a 40°C water bath, and 2 mL of a 7% trifluoroborane methanol solution And 1 mL of toluene are added. After sealing, it is heated in an oven at 100° C. for 45 minutes and then cooled at room temperature. After adding 5 mL of distilled water, 1 mL of hexane, and 1 g of anhydrous sodium sulfate, the mixture was shaken and left to stand. The separated supernatant was taken into another vial containing 1 g of anhydrous sodium sulfate, dehydrated, and used for a gas chromatography.

The condition of the gas chromatography device (SHIMADZU GC-2010) for performing the gas chromatography was SP-2560 (100 m X 0.25 mm X 0.2 µm) for the column, the temperature of the injection part was 225°C, and the column temperature was 100 After holding at ℃ for 4 minutes, the temperature was raised to 240 ℃ at a rate of 3 ℃ / min and then maintained for more than 15 minutes, the detector temperature was 285 ℃, the flow rate was 0.75 mL/min helium, the split ratio was 200:1 Was. From the above analysis results, larvae, squid liver oil and control were confirmed in Dongae, etc. containing 5% (w/w) of chlorella, which showed optimal growth, and the results are shown in Table 2.

Type of fatty acid Fatty acid content (g/100g) Chlorella Contrast Example Capric acid 0.02 0.4 0.9 Lauric acid 0.05 9.27 17.95 Myristic acid 0.73 1.83 2.68 Oleic acid 0.99 4.33 4.94 Linoleic acid 0.54 3.6 4.65 Eicosapentaenoic acid (EPA) 5.58 0.02 0.05 Docosahexaenoic acid (DHA) 0 0 0

As shown in Table 2, the content of lauric acid, which is a saturated fatty acid of larvae (control), was 9.27 g/100 g to Dongae, etc., which were supplied only with chick feed, while Dongae supplied 15% (w/w) of chlorella. In the larvae on the back (Example), it increased about 1.9 times to 17.95 g/100 g, the content of oleic acid, an unsaturated fatty acid, increased from 4.33 g/100 g to 4.94 g/100 g, and the linoleic acid content was 3.60 g. Increased from /100g to 4.65 g/100g, and Eicosapentaenoic acid (EPA) increased by about .55 times from 0.02 g/100g to 0.05 g/100g, and the unsaturated fatty acid contained in chlorella was transferred to the body of the larvae to the larvae. It was found that the content of unsaturated fatty acids increased.

In particular, EPA, which is abundantly contained in chlorella, is an essential omega-3 fatty acid in cultured fish, and it is well transferred to larvae in Dongae and so on, and increased by about 2.5 times compared to the control, so it was judged that the overall omega-3 unsaturated fatty acid increased.

Therefore, it was judged that the larvae of Dongae et al. fed chlorella would be sufficient for the feed of functional farmed fish enriched with unsaturated fatty acids, especially omega 3 fatty acids.

From the above results, the larvae of Dongae, etc., supplied with chlorella, have increased fatty acid content compared to the larvae of existing Dongae, etc., and in particular, the content of omega 3 fatty acids is increased, so feed composition, in particular, aquaculture feed for sheep-season fish where omega 3 fatty acids are essential When the effect of chlorella, which can inhibit the growth rate of larvae in Dongae, etc., is added in the whole feed composition in terms of economics, it is very good efficiency, that is, it is significantly different from the control. Since it was confirmed that there was no effect of inhibiting the gain rate, based on the total weight, Dongae, etc. bred with a feed containing 15% by weight (w/w) of chlorella were used as a raw material for aquaculture feed. It was evaluated as suitable for use.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

Claims (9)

Dongae etc. bred as a feed composition in Dongae including chlorella as an active ingredient, EPA (Eicosapentaenoic acid) content is 0.05% (w/w) and linoleic acid content is 4.65% ( w/w), the content of lauric acid is 17.95% (w/w), the content of oleic acid is 4.94% (w/w), and the content of myristic acid is 2.68% To Dongae etc. characterized in that (w/w). The method of claim 1,
The composition for feed in Dongae and the like is Dongae, characterized in that 15% by weight (w/w) of chlorella is contained, based on the total weight of the composition for feed in the Dongae, etc. A feed composition comprising the Dongae et al. of claim 1 as an active ingredient.
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