KR102151740B1 - Cosmetics composition comprising gold nano rod and titanium dioxide for preventing near infrared ray and heat - Google Patents

Abstract

The present invention relates to a cosmetic composition for shielding near-infrared rays and heat comprising a dog bone gold nanorod, in which titanium dioxide is mixed to shield heat transfer caused by penetration of near infrared rays, thereby inhibiting a temperature rise in the skin, and in which the expression of human matrix metalloproteinase-1 (MMP1) and the production of reactive oxygen species (ROS) are inhibited to suppress skin aging, skin damage or the like, and thus can be easily used as a cosmetic composition for improving skin conditions.

Description

Cosmetics composition comprising gold nano rod and titanium dioxide for preventing near infrared ray and heat

The present invention relates to a near-infrared and thermal barrier cosmetic composition containing gold nanorods and titanium dioxide. The gold nanorod is a rod type or a dog-bone type, and according to the present invention, titanium dioxide is included in the gold nanorod to scatter and refract light in the near-infrared region to block thermal shear to the skin. Thus, it provides a near-infrared and heat-blocking cosmetic composition that can improve various skin aging caused by this.

Aging by sunlight is divided into photoaging by UV and thermal aging by near infrared rays. Photoaging is associated with rough wrinkles, uneven pigmentation, loss of skin elasticity, disturbance of skin barrier function, skin cancer, etc., and is a skin aging phenomenon that can be prevented by avoiding exposure to ultraviolet rays (Korean Patent Registration No. 10-1148427). Ultraviolet rays are divided into UVA, UVB, and UVC depending on the wavelength, among which, UVA is 95% and UVB is 5%. UVA, which has the longest wavelength in the UV region, is a direct cause of photoaging because it can reach the dermal layer. Commercially available sunscreens are manufactured by mixing a chemical sunscreen such as ethylhexylmethoxycinnamate or a physical sunscreen such as ZnO and TiO ₂ . Recently, it was found that near-infrared rays (700-3000 nm), which constitute about 54% of solar energy, are one of the environmental factors that promote skin aging (thermal aging) by increasing the skin temperature. In Seoul, it was mentioned that the temperature in the dermis rises by more than 40℃ in 20 minutes under the midday sunlight of the summer [J Investing Dermatol Symp Proc., 14(1), pp. 9-15, 2009; Seo JY, Chung JH. Thermal aging: A new concept of skin aging., Journal of Dermatological Science Supplement, 2(1), 13-22, 2006]. However, like UVA, IRA also releases Reactive Oxygen Species (ROS) when it penetrates the skin, thereby promoting the expression of various proteins. When the human body is exposed to sunlight, the UV region is absorbed by Oxyhemoglobin (HbO ₂ ), Deoxyhemoglobin (Hb), Melanin, etc. present in the blood in large quantities, and the infrared region is absorbed by water in the blood. However, since there is almost no material in the body that can block the near-infrared (NIR) region (700-1400 nm), the human body is called the NIR window [WIREs Nanomed. Nanobiotechnol., 2(5), 461-477, 2010].

To block such near-infrared rays, a heat shield must be manufactured and used using a material that scatters or absorbs light. The method of blocking heat by absorbing near-infrared rays is a method of using semi-conductors or metal nanoparticles capable of absorbing the region. However, in the case of semi-conductors such as CdSe or GaAs, which have a bandgap energy of 0.9-1.7 eV in the near-infrared region, biocompatibility is low, and most metal nanoparticles such as Cu nanorods and Ag nanorparticles are also difficult to apply to cosmetics due to biotoxicity. .

The domestic and overseas cosmetics industry is striving to develop total sun care cosmetics that block UVA-IRA at the same time, and through this, various UVA-IRA blockers have been developed. However, heat blockers currently released in the industry use materials with a large refractive index to physically It only blocks heat by scattering light, and there has been no report in the academic world as to whether these products have infrared blocking capabilities [Skin Pharmacol Physiol., 23(1), 7-15, 2010]. Therefore, unlike heat blocking products on the market in Korea, the present invention intends to block heat by using a material that absorbs the near-infrared region.

Metal nanoparticles such as gold form surface plasmon resonance (SPR) by vibrating free electron clouds through the interaction of free electrons existing on the surface with electromagnetic waves of external light. For example, gold nanoparticles having a particle size of about 10 nm satisfy the SPR condition in light of about 520 nm wavelength according to Mie theory and cause strong extinction (Scattering + Absorption) (Phys. Chem. Chem. Phys. , 7, 3258-3268, 2005; Solid State Physics, Brooks Cole, 1976). Spherical gold nanoparticles have the same SPR phenomenon in all directions, whereas gold nanorods have different paths in which electron clouds vibrate in the vertical and horizontal directions due to external electromagnetic waves, and the SPR condition occurring in the vertical axis is the horizontal direction. It is caused by light with a longer wavelength than the SPR condition of. That is, as the aspect ratio increases, that is, as the length of the gold nanorod increases, the absorption peak at a long wavelength causes a red-shift, and generally at a wavelength in the near-infrared region of about 700 to 1,200 nm depending on the aspect ratio. Causes strong absorption. At this time, if strong near-infrared light is irradiated onto the gold nanorod, free electrons rapidly vibrate along the longitudinal axis of the gold nanorod and collide with the gold atom and are converted into heat. In this way, the process of converting photon energy into heat energy It is called the photothermal effect. On the other hand, gold nanorods are formed of a dog-bone gold nanorod (see FIG. 1B) having irregular at both ends of the rod shape (see FIG. 1A) in a smooth shape (see FIG. It can also be manufactured in form.

On the other hand, the present inventors possess the technology for a near-infrared ray blocking cosmetic composition containing gold nanorods in Korean Patent No. 10-1776140, and thus, while studying various features of gold nanorods, biocompatibility is excellent. The present invention was completed by confirming that the cosmetic composition prepared by mixing TiO ₂ in a gold nanorod capable of absorbing the near-infrared region significantly reduced the amount of gold used as a raw material and had excellent heat shielding effect.

Republic of Korea Patent Registration No. 10-1148427 (Name of invention: composite powder for simultaneous blocking of infrared and ultraviolet rays and cosmetic composition using the same, Applicant: Koreana Cosmetics Co., Ltd., registration date: May 11, 2012) Republic of Korea Patent Registration No. 10-1333962 (Name of invention: Gold nanorod manufacturing method, Applicant: Keumo University of Technology Industry-Academic Cooperation Foundation, registration date: November 21, 2013) Republic of Korea Patent Registration No. 10-1776140 (Name of invention: Near-infrared blocking cosmetic composition and near-infrared blocking agent, Applicant: Keumo Institute of Technology Industry-Academic Cooperation Foundation, registration date: September 1, 2017)

Cho, S, Shin MH, et al. Effects of infrared radiation and heat on human skin aging in vivo., J Investing Dermatol Symp Proc., 14(1), 9-15, 2009. Seo JY, Chung JH. Thermal aging: A new concept of skin aging., Journal of Dermatological Science Supplement, 2(1), 13-22, 2006. Altinoglu Erhan I., Adair James H., Near infrared imaging with nanoparticles. WIREs Nanomed. Nanobiotechnol., 2(5), 461-477, 2010. Schoroedder P, Calles C, et al., Photoprotection beyond ultraviolet raidation-effective sun protection has to include protection against infrared A radiation-induced skin damage., Skin Pharmacol Physiol., 23(1), 7-15, 2010. Vericat C, Vela ME, and Salvarezza RC, Phys. Chem. Chem. Phys., 7, 3258-3268. 2005. Ashcroft NW and Mermin ND, Solid State Physics, Brooks Cole, 1976. Linfeng Gou and Catherine J. Murphy, Fine-Tuning the Shape of Gold Nanorods, Chem. Mater., 17, pp. 3668-3672, 2005. K.-D. Shim and E.-S. Jang, Bull. Korean Chem. Soc., 2018. Chandramouli Subramaniam Renjis T. Tom T. Pradeep, On the formation of protected gold nanoparticles from AuCl4- by the reduction using aromatic amines, Journal of Nanoparticle Research, Vol. 7, pp. 209-217, 2005. Cosmetics Research Team, Medical Product Research Department, Guidelines for Analysis of Limit Components in Cosmetics, Ministry of Food and Drug Safety, 2016.

An object of the present invention is to provide a near-infrared and thermal barrier cosmetic composition containing gold nanorods and titanium dioxide. When the gold nanorod is a rod type, it is a dog-bone type, and contains titanium dioxide to scatter and refract light in the near-infrared region to block thermal shear to the skin, thereby improving various types of skin aging. It provides a cosmetic composition for near-infrared and heat shielding.

The present invention relates to a near-infrared and thermal barrier cosmetic composition containing gold nanorods and titanium dioxide.

The gold nanorod may be a gold nanorod having a rod shape or a dog bone shape.

The length of the gold nanorod may be 30 ~ 1000nm.

The composition is characterized in that it blocks a light source of a wavelength in the near-infrared range of 700 to 1,200 nm.

The cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing It is characterized by having a formulation selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

Hereinafter, the present invention will be described in detail.

The dog bone gold nanorod is a type of gold nanorod having a dog bone shape, and preferably has an aspect ratio of 3 to 6. When the aspect ratio of the dogbone gold nanorod is less than 3 or exceeds 6, the heat transfer efficiency of the patch composition may decrease. The dogbone gold nanorods may have a length of 30 to 1000 nm, preferably 30 to 500 nm, and most preferably 40 to 45 nm.

The particle size of the titanium dioxide is characterized in that 200 ~ 400 nm.

Various functional substances may be supported in the cosmetic composition of the present invention, and any of the functional substances may be used as long as it can be delivered to the skin. Preferably, it may be one or more drugs selected from the group consisting of anti-wrinkle agents, whitening agents, antioxidants, anti-inflammatory agents, hair growth agents, allergy improving agents, and atopy improving agents. The functional material may be a natural compound, a natural extract, a powder of a natural product, a synthetic compound, a natural peptide, a recombinant peptide, a microorganism or a culture solution thereof, a mineral, or the like. In this case, especially as a wrinkle improving agent, as a substance that inhibits MMP-1, an extracellular matrix (ECM) proteolytic enzyme, silicic acid, N-Methyl-L-serine , Isoflavonoids, Dehydroepiendrosteron, Paoniflorin, etc. can be selected. Benzastatin is a substance that prevents skin aging by removing free radicals that promote the breakdown of ECM. (Benzastatins), Coenzyme Q10, etc. can be used.In addition, adenosine, ascorbyl glucoside, kinetin, auxin, peptides, which are known for various wrinkle improvement effects, etc. (peptide), retinol (retinol), retinyl palmitate (retinyl palmitate), polyethoxylated retinamide (Polyethoxylated Retinamide), alpha-hydroxy acid (alpha hydroxyl acid) and the like can be used. As the whitening agent, the whitening agent is arbutin, niacinamide, ascorbic acid, magnesium ascorbyl phosphate, ascorbyl acid-2-glucoside. , Japanese oak extract, ethyl ascorbyl ether, oil-soluble licorice extract, etc. In addition, collagen, various vitamins, etc. may be added as the drug, promoting epidermal cell proliferation and having a moisturizing effect. Oxyproline and the like may be included.

The cosmetic composition is characterized in that it blocks a light source of a wavelength in the near-infrared range of 700 to 1,200 nm.

In the preparation of the composition of the present invention, the dog bone gold nanorods may be prepared in more detail by the following method.

That is, (1st step) hexadecyltrimethylammonium bromide (CTAB) aqueous solution and benzyldimethylhexadecylammonium chloride (BDAC) to prepare a mixed solution,

Add sodium borohydride aqueous solution (NaBH ₄ ), hexadecyltrimethylammonium bromide (CTAB) and chloroauric acid (HAuCl ₄ ) aqueous solution to make gold seeds and store them at 20~40℃. Aging the gold seed; And,

(2nd step) In the mixed solution prepared in the first step, chloroauric acid (HAuCl ₄ ), silver nitrate (AgNO ₃ ), ascorbic acid aqueous solution, and the gold seed produced aged in the first step, and chloride Geumsan (HAuCl ₄ ) is mixed and stirred for 18 to 48 hours, centrifuged to remove the upper layer solution, and then the remaining solution is dispersed in distilled water to obtain a solution containing Dog bone gold nanoload. Step; may include. At this time, each stirring time or mixing time in each step may be 1 minute to 48 hours, and centrifugation is preferably performed at 200 to 2000 rpm.

Even more preferably,

(1st step) 1~2mM benzyldimethylhexadecylammonium chloride (BDAC) 8~12ml based on 10ml of 0.1~0.2M hexadecyltrimethylammonium bromide (CTAB) aqueous solution To prepare a solution,

0.01 ~ 0.06M sodium borohydride (NaBH ₄ ) 2~8ml, 0.005~0.2M hexadecyltrimethylammonium bromide (CTAB) 3~7ml and 25~50mM chloroauric acid (HAuCl ₄₎ ) Aging gold seeds by adding 10-50 µl of aqueous solution to make gold seeds and storing them at 20-40° C. for 0.5-3 hours; And,

(Step 2) In the mixed solution prepared in Step 1, 25 to 50 mM chloroauric acid (HAuCl ₄ ) 100 to 500 μl, 25 to 50 mM silver nitrate (AgNO ₃ ) 20 to 80 μl, 30 to 80 mM ascorbic acid 100-500 µl of aqueous solution and 10-70 µl of 40-50mM chlorinated acid (HAuCl ₄ ), prepared aged in the first step, were mixed, stirred for 18-48 hours, and then centrifuged to the upper layer solution. After removing the, the remaining solution is dispersed in distilled water to obtain a solution containing a dog bone gold nanoload. It may include.

At this time, the gold concentration of the dogbone gold nanorod solution obtained in the second step is preferably 100 to 5000 ppm, and it is preferable to dilute it appropriately for application as a cosmetic composition.

In the second step, a washing step of centrifuging the solution containing the dog bone gold nanorods again, removing the upper layer solution, and dispersing the remaining solution in distilled water may be added 1 to 3 times, through which hexadecylcetyltrimethyl Ammonium bromide is removed to obtain a more purified solution containing dogbone gold nanorods.

In the cosmetic composition of the present invention, dog bone gold nanorods or gold nanorods may be included in an amount of 0.015 to 0.03 wt%, and titanium dioxide is preferably included in an amount of 5 to 10 wt%.

When the concentration of dogbone gold nanorods or gold nanorods included in the cosmetic composition is less than 0.015 wt%, near-infrared rays and heat blocking may not occur. Even if it exceeds 0.03 wt%, since the final prepared composition does not significantly increase near-infrared rays and heat shielding effects, it is not preferable in terms of manufacturing cost. In addition, it may not be appropriate because the formulation as a cosmetic composition may not be well formed.

Likewise, when the concentration of titanium dioxide contained in the cosmetic composition is less than 5 wt%, near-infrared rays and heat blocking may not occur. Even if it exceeds 10 wt%, since the final prepared composition does not significantly increase near-infrared rays and heat shielding effects, it is not preferable in terms of manufacturing cost.

The general gold nanorods usable in the present invention are in the form of a rod and have an aspect ratio of 2 to 7. When the aspect ratio of the gold nanorods is less than 2 or exceeds 7, the heat transfer efficiency of the patch composition may decrease. The gold nanorods may have a length of 30 to 1000 nm, preferably 30 to 500 nm.

The manufacturing method for this is similar to dogbone gold nanorods, and boron hydride in a mixture of an aqueous solution of hexadecylcetyltrimethylammonium bromide and an aqueous solution of hydrogen tetrachloroaurate (III) tetrahydrate. A solution containing gold seed was prepared by adding an aqueous sodium solution,

After mixing an aqueous chloroauric acid solution, a mixed aqueous solution of hexadecylcetyltrimethylammonium bromide and benzyldimethylhexadecylammonium chromide, an aqueous solution of silver nitrate as a catalyst for the synthesis of gold nanorods and an aqueous solution of ascorbic acid as a reducing agent were added under stirring to prepare a growth solution. Manufacturing steps;

(Second step) mixing and stirring the solution containing the gold seed and the growth solution, centrifuging to remove the upper layer solution, and dispersing the remaining solution in distilled water to obtain a solution containing gold nanorods; including can do.

At this time, in the first step, the volume and concentration of each solution are not largely limited, but preferably the hexadecylcetyltrimethylammonium bromide aqueous solution is 0.05-5 mM, hydrogen tetrachloroaurate (III) tetrahydrate. The aqueous solution is preferably 0.05 to 5 mM, the sodium borohydride aqueous solution is preferably one having a concentration of 1 to 50 mM, and the mixing ratio of each solution is preferably 1:0.1 to 5:0.05 to 0.5 by volume.

In addition, in the first step, the solution containing the gold seed is preferably 90 minutes or more after preparation, and the time for that time is not greatly limited, but it is preferably aged for 90 minutes to 6 hours.

In the second step, the solution containing the gold seed and the growth solution of the gold nanorods may be mixed in a volume ratio of 1: 500 to 2000.

In the second step, the volume and concentration of each solution or sample for preparing the growth solution is also not significantly limited, but preferably 300 to 3000 ml of chloroauric acid (HAuCl ₄ ·4H ₂ O) of 0.5 to 5 mM , An aqueous solution of hexadecylcetyltrimethylammonium bromide (CTAB) and 30benzyldimethylhexadecylammonium chromide (BDAC) mixed with 40-300 mM hexadecylcetyltrimethylammonium bromide (CTAB) and 30-70 mM benzyldimethylhexa After mixing with 300-3000 ml of decylammonium chloride (BDAC)), 20-70 ml of 1-10 mM silver nitrate, which is a catalyst for the synthesis of gold nanorods, was added, and 10-20 ml of 50-100 mM ascorbic acid aqueous solution as a reducing agent was added under stirring. It can be prepared by adding.

In the second step, a washing step of centrifuging the solution containing gold nanorods again, removing the upper layer solution, and dispersing the remaining solution in distilled water may be added 1 to 3 times, through which hexadecylcetyltrimethylammonium bromide Is removed to obtain a solution containing more purified gold nanorods.

The formulation of the cosmetic composition of the present invention is not particularly limited, but preferably skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, It may be selected from the group consisting of foundation, essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any one that can be blended in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. And their salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) are also included in the water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme method or a chemical synthesis method.

As the oil-soluble vitamin, any one may be used as long as it can be blended in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , Their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, dl-pantotenyl alcohol, D-pantotenyl alcohol, pantotenyl ethyl Ether, etc.) are also included in the oil-soluble vitamin used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a microorganism culture, an enzyme or a chemical synthesis method.

The polymer peptide may be any one as long as it can be blended into a cosmetic, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are exemplified. The polymeric peptide can be purified and obtained by a conventional method such as a purification method from a culture medium of a microorganism, an enzyme method, or a chemical synthesis method, or it can be used after being purified from natural products such as dermis of pigs and cattle, silk fibers of silkworms.

The polymer polysaccharide may be any one as long as it can be blended in the cosmetic composition, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be any one as long as it can be blended into the cosmetic composition, and ceramide, phytosphingosine, sphingoglycolipid, and the like are preferably mentioned. Sphingo lipids are usually purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method, or can be obtained by chemical synthesis.

The seaweed extract may be anything as long as it can be blended into the cosmetic composition, but preferably brown algae extract, red algae extract, green algae extract, etc. are exemplified, and carrageenan, arginic acid, sodium arginate purified from these seaweed extracts And potassium arginate are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, other ingredients to be blended in conventional cosmetics may also be blended.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, Blood circulation accelerators, cold sensation agents, restrictors, and purified water.

Examples of oil and fat components include ester oils and fats, hydrocarbon oils, silicone oils, fluorine oils, animal oils and vegetable oils.

Examples of ester oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, tri2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra2-ethylhexanoate pentaelisitol , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristic acid, myristic myristic acid, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostea laurate Reel, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate cetoste Aryl, 2-ethylhexanoate stearyl, isostearate hexyl, dioctanoate ethylene glycol, dioleate ethylene glycol, dicaprylic acid propylene glycol, dicaprylic acid propylene glycol, dicaprylic acid propylene glycol, dica Neopentyl glycol prinate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerololeic acid ester, polyglycerin isostearate, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, di malic acid Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, pitsteryl isostearate, pitsteryl oleate, 12-stealoyl And esters such as isocetyl hydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and petrolatum.

Silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylsteaoxysiloxane copolymer, alkyl And modified silicone oil and amino-modified silicone oil.

As fluorine-based fats and oils, perfluoropolyether, etc. are mentioned.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed Oil, Palm Oil, Cucuine Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Walnut Colostrum Oil, Marker Demi Anut Oil, Meadow Home Oil, Egg Yolk Oil, Tallow Oil, Horse Oil, Mink Oil, Orange Rape Oil, Jojoba Oil , Animal or plant fats such as canderry wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular weight moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactate, and the like.

As a fat-soluble low molecular weight moisturizer, cholesterol, cholesterol ester, etc. are mentioned.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, and the like. I can.

Examples of the oil-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, POE·POP alkyl ether, polyether-modified silicone, lauric acid Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

As anionic surfactants, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide Phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkylether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, and the like. have.

Cationic surfactants include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyldimethylbenzyl ammonium chloride, behenyl trimethyl ammonium bromide, and chloride. Benzalkonium, diethylaminoethyl amide stearate, dimethylaminopropyl amide stearate, lanolin derivative quaternary ammonium salt, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. Amphoteric surfactants, and the like.

As organic and inorganic pigments, silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide , Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene-styrene copolymer, Organic pigments, such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments, etc. are mentioned.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as sodium zinc cetylate, a zinc laurylate, and calcium laurylate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lizine, N-alpha-paritoylolnitine, N-alpha-lauroylarginine, N-alpha-hardened tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, ethylene tetrafluoride, and the like.

UV absorbers include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicate, butyl phenyl salicylate, homomentyl salicylate, benzyl cinnamate , Paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl·diisopropyl cinnamic acid ester mixture, right Cannic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

As a disinfectant, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinphylithione, benzalkonium chloride, photosensitization Sono No. 301, mononitroguarous sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and elisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmalic acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending ingredients that may be added are not limited thereto, and any of the above ingredients can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5% by weight, more preferably, based on the total weight. May be blended in 0.01-3% by weight.

The cosmetic composition of the present invention may take the shape of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetics in addition to various compositions derived from the strain as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments, and fragrances. It includes conventional adjuvants and carriers.

When the cosmetic composition formulation of the present invention is a paste, cream, or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide, etc. are used as carrier components. Can be used.

When the cosmetic composition formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbons , Propane/butane or a propellant such as dimethyl ether.

When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the cosmetic composition formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the cosmetic composition formulation of the present invention is a surfactant containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

The present invention relates to a near-infrared and heat-blocking cosmetic composition containing dogbone gold nanorods, and by mixing titanium dioxide with dogbone gold nanorods, heat transfer due to near-infrared transmission is blocked, thereby suppressing the increase in skin temperature and MMP1 By inhibiting the expression of (Human Matrix Metalloproteinase-1) and the production of ROS (Reactive Oxygen Species), it can be easily used as a cosmetic composition that improves skin conditions by inhibiting skin aging or skin damage.

Korean Patent Registration No. 10-1333962 and Korean Patent Laid-Open No. 10-2015-0122869, which are prior documents, disclose techniques related to a method of manufacturing gold nanorods, cosmetics for blocking near infrared rays using the same, but dogbone gold nanorods and The near-infrared blocking effect of a mixture of titanium dioxide is still unknown.

1 shows TEM photographs (a, b) and UV-Vis absorption spectra (c, d) of gold nanorods (GNR) and dog bone gold nanoloads.
Figure 2 shows the mechanism by which gold ions are additionally reduced by ascorbic acid and DGNR is formed.
3 shows the standard curve of Gold and the concentration of GNR and DGNR using AAS (Atomic absorption spectroscopy).
4 shows a TEM photograph and UV-Vis absorption spectrum of GNP (Gold nanoparticles).
5 is a TEM photograph and SEM photograph of ZnO and TiO ₂ , Fe ₂ O ₃ , (a) is a TEM photograph of conventional ZnO (50 nm), (b) is a SEM photograph of 300 nm ZnO, (c) Is a SEM image of 600 nm ZnO, (d) is a TEM image of 300 nm TiO ₂ , and (e) is a TEM image of 100 nm Fe ₂ O ₃ (red).
6 shows a graph of the UV-Vis absorption spectrum of ZnO.
7 shows an X-ray diffraction pattern (XRD) graph of TiO ₂ .
8 shows an X-ray diffraction pattern (XRD) graph of Fe ₂ O ₃ .
9 shows a cream application amount (left) and a cream application area (right) containing DGNR.
Figure 10 shows a graph of temperature change when the near-infrared rays are transmitted through the cream-coated slide glass, (a) is DGNR, (b) is ZnO (small), (c) is TiO ₂ , (d) is Fe ₂ Results for creams containing O ₃ .
11 shows a graph confirming the temperature change when the near-infrared rays were transmitted through a slide glass coated with a cream containing TiO ₂ and ZnO.
FIG. 12 is a graph showing a change in temperature when a near-infrared ray is transmitted when a cream is applied by DGNR concentration after fixing the contents of TiO ₂ and ZnO.
FIG. 13 is a graph showing a change in temperature when a near-infrared ray is transmitted when a cream is applied by TiO ₂ and ZnO concentration after fixing the concentration of DGNR.
14 is a graph showing the results of confirming the heat shielding effect according to the ZnO size and the presence or absence of DGNR when (a) is the transmittance according to the size of ZnO, and (b) and (c) are the results of confirming the heat shielding effect according to the size of ZnO and when the near-infrared ray is transmitted through the cream-coated slide glass .
15 is a graph showing the results of near-infrared transmission temperature change for a cream to which GNP is added by concentration after fixing the content of TiO ₂ .
FIG. 16 is a graph showing a list of functional ingredients having a solar/heat blocking function of Prototype 1 and Prototype 2, and a graph confirming the change in transmission temperature for DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%), Prototype 1, Prototype 2 cream Show.
17 is a graph showing the cell death rate results according to the irradiation time of the near-infrared lamp.
18 shows the results of observation of apoptosis when irradiated with near-infrared rays for 10 minutes, Calcein AM (Green) means Live cells and PI (Red) means Dead cells.
19 shows the results of observation of apoptosis when irradiated with near-infrared rays for 20 minutes, Calcein AM (Green) means Live cells and PI (Red) means Dead cells.
20 shows the results of observation of cell death when irradiated with near-infrared rays for 30 minutes, Calcein AM (Green) means Live cells, and PI (Red) means Dead cells.
21 shows an observation image of ROS (Reactive Oxygen Species) when irradiated with near-infrared rays for 10 minutes.
22 shows an ROS observation image when near-infrared rays are irradiated for 20 minutes.
23 shows an ROS observation image when irradiated with near infrared rays for 30 minutes.
24 is a graph showing the result of measuring the expression amount of MMP-1 (Matrix metalloproteinase-1) by near infrared rays.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the content introduced herein is thorough and complete.

<Example 1. Preparation of gold nanorods (Gold nanorods, GNR) and dog bone gold nanoloads (DGNRs)>

Example 1-1. Preparation of gold nanorod (GNR)

To synthesize gold nanorods (GNR), first, gold seeds were synthesized. Gold seed was mixed with 5 ml of 0.5 mM hexadecylcetyltrimethylammonium bromide (CTAB) aqueous solution and 5 ml of 0.5 mM hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl ₄ ·4H ₂ O) aqueous solution and 0.01 M NaBH ₄ solution cooled to about 4℃. After adding 0.6 ml, Vortexing for 3 minutes was performed to prepare a solution containing 2 to 3 nm gold seeds. The synthesized seed solution was used for GNR growth by aging for at least 2 hours and 30 minutes at room temperature before use.

A GNR growth solution was prepared for preparing gold seeds with GNR. First, 1 mM of HAuCl ₄ 4H ₂ O An aqueous solution of 1,000 ml of aqueous solution mixed with hexadecylcetyltrimethylammonium bromide (CTAB) and Benzylhexadecylammonium chloride (BDAC) (CTAB 150 mM, Benzylhexadecylammonium chloride 50 mM, BDAC/CTAB is about 1/3) and 1,000 ml of GNR synthesis catalyst AgNO ₃ 4 mM (50 ml) aqueous solution was added, and 79 mM (14 ml) of an aqueous solution of ascorbic acid as a reducing agent was added under stirring. At this time, the color of the solution changes from dark yellow to colorless as Au(III) is reduced to Au(I).

Next, if 2.4 ml of the matured gold seed solution is added to the GNR growth solution (2064 ml) under stirring, the color of the solution changes from colorless to wine color within about 1 hour. 3~7, length: about 30~60nm, width: about 10~15nm) was obtained.

Next, in order to remove excess CTAB in this solution, centrifugation was performed at 15,000 rpm for 20 minutes, the upper layer solution was removed, and the remaining solution was dispersed in 200 ml of distilled water. This washing process was repeated twice to adjust the final GNR solution to 2000 ppm and dispersed in distilled water. At this time, the size of the synthesized GNR is confirmed to be about 60 nm.

Example 1-2. Preparation of Dog bone gold nanoload (DGNR)

To synthesize dog bone gold nanoload, a solution was prepared by mixing 10 ml of 0.1M hexadecyltrimethylammonium bromide (CTAB; Sigma Aldrich) and 10 ml of 1mM benzyldimethylhexadecylammonium chloride (BDAC; Sigma Aldrich).

In another container, 0.058M NaBH₄ 5ml (JUNSEI), 0.1M CTAB 5ml and 50mM HAuCl₄30µl (Sigma Aldrich) were mixed to make seeds and stored at room temperature for one hour.

Then, 50mM HAuCl₄300µl (Sigma Aldrich), 50mM AgNO₃ 44.8µl (Sigma Aldrich), and 80mM Ascorbic acid 300µl (Sigma Aldrich) were added to the solution. Subsequently, the Seed synthesized above and 50mM HAuCl₄40µl were added, followed by stirring for 24 hours to synthesize.

The dogbone gold nanorods prepared in this way (aspect ratio of about 3 to 5, length: about 40 to 45 nm, width: about 10 to 15 nm) were centrifuged to remove the upper layer solution, and then the remaining solution was diluted in distilled water to adjust to 2000 ppm. It was dispersed in distilled water. At this time, the size of the synthesized DGNR is confirmed to be about 40 ~ 45nm.

The results of Examples 1-1 and 1-2 are shown in [Fig. 1], and (a) and (b) of [Fig. 1] show TEM (transmission electron microscope) photographs of GNR and DGNR. DGNR has a wider shape at both ends compared to GNR, and the aspect ratio of GNR was observed to be an average of 3.99 (± 0.866). (c) and (d) show UV-vis absorption spectra. In both DGNR and GNR, a strong longitudinal peak was observed around 750nm and a weak transverse peak was observed around 500nm. In the spectrum of DGNR, unlike GNR, it can be seen that the peak observed around 500nm is split, and two peaks are observed as the end of the rod widens.

The concentration ratio of the ingredients in the growing solution of GNR and DGNR and the concentration of other ingredients based on Gold Condition GNR DGNR Concentration (mM) Concentration ratio
(Au standard) Seed amount (µl) Concentration (mM) Concentration ratio
(Au standard) Seed
Amount (µl) HAuCl ₄ 0.4841 One 120 0.7252 One 200 AgNO ₃ 0.0968 0.2 0.1160 0.16 Ascorbic Acid 0.5339 1.1 1.1603 1.6

[Linfeng Gou and Catherine J. Murphy, Fine-Tuning the Shape of Gold Nanorods, Chem. Mater., 17, pp. 3668-3672, 2005], in the case of gold nanorods in which the ratio of AA (Ascorbic acid) and Au salt (HAuCl ₄ ) is about 1:1, there is about 40% of remaining Au ions in the solution, but AA: Au salt In the case of DGNR synthesized at a ratio of = 3: 2, it is known that Au ions are mostly reduced by excess AA, and are bound to a relatively small CTAB-bound surface to form a dog-bone form (FIG. 2). For this, reference may be made to [Table 1]. Table 1 is a table showing the concentrations of components in the growing solution that cause differences in the shape of GNR and DGNR and the concentration ratios of other components based on Gold.

[Fig. 3] shows the standard curve of Gold measured using AAS (Atomic absorption spectroscopy). 100 ml of DGNR and GNR were synthesized, washed, and then dispersed in 100 ml. The absorbance was measured using AAS. As a result, the absorbance was 0.014 and 0.030, respectively, and as a result of substituting into the formula shown in Fig. 3, DGNR It was found that the concentration of was more than twice as thick as that of GNR. This is because when synthesizing DGNR, not only more Au seeds are added than GNR, but also most of Au ions are reduced by a higher ratio of AA.

Therefore, since there is an economical advantage of the synthesis process and the synthetic raw materials, further experiments were conducted using DGNR.

Example 1-3. Preparation of gold nanoparticles (GNP)

Gold nanoparticles (GNP) manufacturing method is described in [Chandramouli Subramaniam Renjis T. TomT. Pradeep, On the formation of protected gold nanoparticles from AuCl ₄ ^- by the reduction using aromatic amines, Journal of Nanoparticle Research, Vol. 7, pp. 209-217, 2005] was synthesized by modifying the method.

To this end, 100 ml of a 1 mM HAuCl ₄ solution was heated under stirring at 500 rpm. When the solution temperature reached 90° C. or higher, 10 ml of 35.4 mM Trisodium citrate dihydrate was added and heated for 10 minutes. At this time, the color of the solution changes from colorless to black and then becomes dark red. After going through the washing process twice, it was dispersed in distilled water at a gold concentration of 2000 ppm.

For Gold nanoparticles prepared in this way, the TEM photograph and UV-vis absorption spectrum results are shown in [FIG. 4]. As a result, spherical nanoparticles having a size of 10-20 nm can be identified on the TEM, and unlike rod-shaped gold nanoparticles, a strong peak is observed at about 520 nm because it has only one oscillation direction.

Example 1-4. ZnO, TiO ₂ , Fe ₂ O ₃ The characteristics of

As additives capable of inhibiting the absorption of near-infrared rays of the skin, ZnO and TiO ₂ used as inorganic sunscreen raw materials for cosmetics, and Fe ₂ O ₃ added as a colorant were selected and their properties were confirmed. ZnO, TiO ₂ , and Fe ₂ O ₃ were commercially available products, and UV-Vis absorption spectrum and X-ray diffraction pattern were measured in order to understand the structure and characteristics (FIGS. 6 to 8).

[Fig. 5] is a result of observing the ZnO, TiO ₂ and Fe ₂ O ₃ particles used in the present invention on TEM and SEM, and the size of ZnO in [Fig. 5a] is 20-50 nm, and ZnO in [Fig. 5b] is 300-400 nm, ZnO in [Fig. 5c] is about 600 nm, TiO ₂ in [Fig. 5d] is 100-300 nm, and Fe ₂ O ₃ in [Fig. 5e] is about 100 nm. This is because the size of ZnO is expected to affect near-infrared blocking, that is, reflection, so ZnO of different size is used. In order to clarify the components of these raw materials, the UV-Vis absorption spectrum was measured for ZnO, and the large size TiO ₂ and Fe ₂ O ₃ could not see the UV-Vis absorption spectrum, so the structure was clarified using the XRD spectrum. According to [Figs. 6] to [8], ZnO can observe a strong peak between 200-300 nm on the UV-Vis absorption spectrum, TiO ₂ is a Rutile structure in XRD, and Fe ₂ O ₃ is a Hematite structure. I can.

<Example 2. Preparation of composition for near-infrared and heat shielding>

Example 2-1. Preparation of cream

Oil, emulsifier, purified water and preservative were prepared as basic ingredients for making cream form. To make a heat blocker, grape seed oil and ceramide, which have a moisturizing effect, were used, and emulsified wax, distilled water, and napri, a natural herbal preservative were added, and then evenly dispersed with a homogenizer to prepare a vehicle (Table 2).

Raw material Amount added (g) Grape seed oil 10 Emulsified wax 1.3 water 20 Ceramide (moisturizer) 0.5 Napri (natural preservative) 0.5

Since the DGNR and GNP solutions are dispersed in water, the total weight was kept constant by reducing the amount of distilled water added when making the cream by the weight added.

According to the Ministry of Food and Drug Safety, the limit of blending TiO ₂ and ZnO ingredients for cosmetics is set at 25 wt%, respectively, and generally used cosmetics contain 5-15 wt% (Medical Product Research Department, Cosmetics Research Team, Analysis method guideline, Ministry of Food and Drug Safety, 2016), TiO ₂ and ZnO were added so as to be 5, 10, 15, 20, 25 wt% of the total weight to prepare a cream. The formulation limit for Fe ₂ O ₃ is not set, but since it is mainly used as a colorant, about 0.1 wt% is added in general cosmetics. Therefore, Fe ₂ O ₃ was prepared by adding 0.1, 0.5, 1, 1.5 wt% based on the total weight of the cream.

In the case of creams to which each additive was added, distilled water was reduced by the amount added so that the total weight was kept constant.

Example 2-2. Preparation of commercially available heat shields

In order to compare each ingredient prepared in Example 1 and Example 2-1 or a cream containing them, a prototype 1 (Conventional product 1; C.Product 1) and a prototype 2 (Conventional product 2; C.Product 2) were purchased. Then, after coating the slide glass at 2 mg/cm ² , the heat shielding effect was tested through a near-infrared transmission test.

<Experimental Example 1. For near-infrared and heat blocking in vitro test>

Experimental Example 1-1. Test method of transmittance and heat barrier effect according to application of cream

The test method of transmittance and heat shielding effect according to application of the cream is presented as follows.

First, as shown in [Fig. 9], a table showing the amount of cream applied and an area where the cream is applied to the slide glass are shown. The cream was applied evenly in an amount of 2 (± 0.00146) mg/cm ² on an area of 1.7 cm wide × 5.0 cm long. When testing the heat shielding effect, light from a near-infrared lamp was transmitted through a slide glass coated with a sample using an IR camera, and the temperature at this time was measured. The temperature of the slide glass without cream is used as a control, and the temperature increase inhibition rate (TRI) is calculated using the temperature of the vehicle or the slide glass with functional cream applied, and the temperature increase inhibition rate of the vehicle and sample The experiment was conducted by comparing the (TRI) values. The starting temperature of the experiment was set based on the room temperature of 29°C, and was conducted for a total of 10 minutes. When measuring the transmittance, after applying the cream to the slide glass, transmission was measured using a UV-Vis spectrophotometer.

In order to test the transmittance, a cream prepared by adding DGNR, TiO ₂ , ZnO, Fe ₂ O ₃ and the like in Example ₂ was prepared as a sample.

The prepared cream sample was evenly applied to a slide glass in an amount of about 2 mg/cm ² , left for 10 minutes, and then the transmittance was measured using a UV-vis spectrophotometer. In order to test the heat shielding effect, the cream-coated slide glass was placed 15 cm apart from the near-infrared lamp so that near-infrared light was transmitted. A Petri dish with a diameter of 120 mm covered with a rubber cover was placed under it, and the distance from the slide glass to the rubber cover was maintained at 10 cm, and then placed so that the light transmitted through the slide glass could reach the rubber cover. After that, the temperature of the rubber cover was measured using an IR camera. The measured temperature value was substituted into [Reaction Formula 1] to calculate the temperature increase inhibition rate (TRI) (refer to Korean Patent Registration No. 10-1595565).

[Scheme 1]

Experimental Example 1-2. DGNR, ZnO, TiO ₂ , Fe ₂ O ₃ Test the heat barrier effect of the cream contained as a single additive

Of DGNR, ZnO, TiO ₂ , Fe ₂ O ₃ In order to determine whether there is a heat barrier effect when 4 types of functional ingredients are added as a single substance, the cream of Example 2-1 is divided into 19 types as shown in [Table 3], and DGNR, ZnO, TiO ₂ , Fe ₂ O ₃ Prepared to be added to conduct a near-infrared transmission experiment.

DGNR (ppm) ZnO (wt%) TiO ₂ (wt%) Fe ₂ O ₃ (wt%) 15 (0.0015wt%) 5 5 0.1 75 (0.0075wt%) 10 10 0.5 150 (0.0150wt%) 15 15 One 225 (0.0225wt%) 20 20 1.5 300 (0.0300wt%) 25 25 -

The temperature change when each cream-coated slide glass was passed through was measured for 10 minutes, and is shown in FIG. 10. [Fig. 10a] shows the temperature change for the DGNR cream, the slide glass increased by 9.8 °C, but the cream to which 0.015 wt% (150 ppm) of DGNR was added increased by 6.86 °C. In the vehicle and DGNR 0.015 wt% (150ppm), the temperature increase inhibition rate (TRI) was 18.98% and 30%, respectively, and at concentrations higher than that, the temperature increase inhibition effect was no longer improved and saturation occurred. [Fig. 10b] is a graph of the temperature change of the ZnO cream. The slide glass increased by 10.22°C, but the cream to which 5 wt% of ZnO was added increased by 8.69°C, and the effect of inhibiting temperature increase was not improved at a concentration higher than that. When the vehicle and ZnO 5 wt%, the temperature increase inhibition rate (TRI) was 6.95% and 14.97%, the difference was 8.02%. [Fig. 10c] is a graph of the temperature change of TiO ₂ cream, where the temperature increase inhibition rate (TRI) at 10 wt% of TiO ₂ is 27.10% and the difference from the vehicle is 15.01%. At higher concentrations, the effect was saturation. [Fig. 10d] is a graph of the temperature change of Fe ₂ O ₃ cream. Even though the concentration was increased, there was no significant difference from the vehicle, but the temperature increase inhibition rate (TRI) at 0.1 wt% was 22.05% and showed a difference of 3.61% from the vehicle. .

On the other hand, when DGNR, ZnO, and TiO ₂ were added, the temperature increase inhibition rate (TRI) showed a difference of 10% or more from the vehicle, but Fe ₂ O ₃ was 3.61%, which was not significantly different from the vehicle. Since the content of Fe ₂ O ₃ is added in trace amounts compared to other materials, it was expected that the reflection effect could not be seen even though the refractive index was larger than that of TiO ₂ or ZnO. Therefore, Fe ₂ O ₃ was judged to be a material with insufficient heat blocking effect, and was excluded from later experiments.

Experimental Example 1-3. DGNR, ZnO, TiO ₂ Test for the heat-blocking effect of the cream contained as a complex additive

Next, the heat blocking effect of the cream containing _two types of DGNR, ZnO, and TiO ₂ as complex additives was confirmed. To this end, after fixing 10 wt% of TiO ₂ and 5 wt% of ZnO, which are the points where the heat blocking effect was saturated in the previous experiment results, the experiment was conducted by adding other raw materials for each concentration.

[Fig. 11] shows the result of the change in the permeation temperature for the cream mixed with TiO ₂ and ZnO. [Fig. 11a] is a result of measuring the temperature change by varying the concentration of ZnO after fixing TiO ₂ at 10 wt%. , Even if the concentration of ZnO was different, the effect of inhibiting temperature increase was not improved, and the temperature increase inhibition rate (TRI) when 25 wt% of ZnO was added was 32.81%, which was not significantly different from that when ZnO was not added (30.61%). . [Fig. 11b] is a graph measured by varying the concentration of TiO ₂ after fixing ZnO at 5 wt%, and the effect was saturated after 10 wt% of TiO ₂ was added. The vehicle's temperature increase inhibition rate (TRI) was 7.73%, when TiO ₂ was not added, the temperature increase inhibition rate (TRI) was 13.99%, and when 10 wt% was added, the temperature increase inhibition rate (TRI) was 23.81%.

In addition, after fixing the content of TiO ₂ or ZnO at 10 wt% and 5 wt%, respectively, the permeation temperature change was measured by irradiating near-infrared rays on a slide glass coated with a cream having a different concentration of DGNR, and is shown in FIG. , [Fig. 12a] is a measurement of temperature change by increasing the concentration of DGNR to 0.0015-0.06 wt% after fixing TiO _{2. When} DGNR is 0.03 wt%, the temperature increase inhibition rate (TRI) is 47.32%, which is 35.67% compared to the vehicle. There is a difference of %. [Fig. 12b] is a measurement of temperature change by varying the concentration of DGNR after fixing ZnO. When the concentration of DGNR is 0.015 wt%, the temperature increase inhibition rate (TRI) is 29.6%, showing a difference of 19.84% from the vehicle. The effect was no longer improved.

Next, based on the results showing the greatest effect when DGNR 300 ppm (0.03 wt%) and a high refractive index raw material were added in the previous experiment, the content of DGNR was fixed at 0.03 wt%, and the content of TiO ₂ or ZnO was adjusted. While changing, the permeation temperature was measured, and the results are shown in [Fig. 13]. [Fig. 13a] is a graph in which the content of TiO ₂ was varied after DGNR was fixed. When TiO ₂ was 10 wt%, the temperature increase inhibition rate (TRI) was 35.26%, showing a difference of 25.81% from the vehicle. [Fig. 13b] is a graph in which the content of ZnO after DGNR is fixed. When ZnO is 5 wt%, TRI is 26.17%, which is 16.17% different from vehicle.

As such, as a result of mixing and using two of the materials with near-infrared blocking function, the cream added with DGNR and TiO ₂ or ZnO was more effective than when each raw material was used alone, which is TiO ₂ or It is believed that the near-infrared rays were scattered by using a high refractive index of ZnO, and the DGNR absorbed the near-infrared rays, and an additional near-infrared blocking effect appeared. Among them, it was found that the effect of inhibiting the temperature increase was the greatest in the cream containing 0.03 wt% of DGNR and 10 wt% of TiO _2, and the effect was not further improved by increasing the content of the ingredients.

The reason why ZnO has less heat shielding effect than TiO ₂ is that the refractive index of ZnO is smaller than TiO ₂ , and it has been determined that there will be an effect of the size.As the size increases, the amount of scattering in the near-infrared region increases due to Mie scattering. The thermal barrier effect of ₂ appeared to be higher than that of ZnO.

Therefore, next, the transmittance of near-infrared rays and the heat shielding effect were tested using ZnO of about 300 nm and 600 nm, and this is shown in FIG. 14. According to [Fig. 14a], when the size of ZnO is about 50 nm, the transmittance is about 80%, and when the size of ZnO is about 50 nm, the transmittance is about 70% and 60%. On the other hand, TiO ₂ exhibits a transmittance of about 40% at 300 nm, and the transmittance is further reduced under conditions in which TiO ₂ and DGNR are mixed. Although the transmittance decreased as the size of ZnO increased, the effect of blocking light in the near-infrared region was smaller than that of TiO ₂ , which has a large refractive index. [Fig. 14b] is a graph for testing the heat shielding effect for a cream prepared by fixing the content of 50 nm and 300 nm, 600 nm ZnO and 300 nm TiO ₂ at 10 wt%, and the temperature rise when ZnO is 50 nm Inhibition rate (TRI) is 15.9%, at 300 nm and 600 nm, it is 23.6% and 30.7%, respectively, TiO ₂ is 37.0% and vehicle is 11.2%. [Fig. 14c] is a graph showing the heat blocking effect for a cream to which 10 wt% of ZnO or TiO ₂ and 0.03 wt% of DGNR are added. At this time, the vehicle's temperature increase inhibition rate (TRI) is 6.8%, 50 nm ZnO is 19.8%, 300 nm ZnO is 34.6%, 600 nm ZnO is 38%, and DGT is 44.4%. As the size of the particles increased, the scattering intensity in the infrared region increased by Mie scattering, which improved the heat shielding effect, but TiO ₂ of the same size showed higher effects in terms of heat shielding effect and transmittance.

Therefore, ZnO or Fe ₂ O ₃ had insufficient effect of inhibiting temperature increase, and when TiO ₂ and DGNR were used in combination, there was a heat blocking effect of about 30% or more. Thereafter, 10 wt% of TiO ₂ and 0.03 wt% of DGNR were mixed to perform a later experiment, which was designated as DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%) .

Experimental Example 1-3. GNP's heat blocking effect test

Next, for comparison with DGNR, gold nanoparticles (GNP) that absorb about 520 nm rather than the near-infrared region were added to test the heat shielding effect in the same manner as DGNR. At this time, the experiment was conducted by adding GNP by concentration instead of DGNR based on the addition of 10 wt% of TiO _2, which had the highest effect as an additive, and the results are shown in [Fig. 15].

[Fig. 14] is a graph of the permeation temperature change for a cream with different concentrations of GNP after fixing TiO ₂ at 10 wt%. Unlike the experiment with DGNR added, there was no difference in the heat blocking effect according to the concentration in GNP. . When only TiO ₂ was added, the temperature increase inhibition rate (TRI) was 24.50%, and when 0.0015 ppm of GNP was added, it was 25.80%, and there was almost no heat blocking effect by GNP. This is because GNP does not absorb light in the near-infrared region, demonstrating that DGNR is sufficiently effective as a functional raw material for a heat shield.

Experimental Example 1-4. Heat blocking effect test of commercial products

In order to compare the DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%) produced in the experiment with a commercially available heat shield, the heat transmitted by transmitting the near infrared rays was measured after applying the cream to the slide glass. The results are shown in [Fig. 16], and a list of functional raw materials having a solar/heat blocking function of Prototype 1 and Prototype 2, as well as the test results of the transmission temperature change for the DGT, Prototype 1, and Prototype 2 cream are presented.

Referring to Fig. 16, the temperature increase inhibition rates (TRI) of Prototype 1 and Prototype 2 are 27.84% and 30.93%, respectively, and the temperature increase inhibition rate of DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%) produced in this study (TRI) showed the best result at 48.45%.

The effect of inhibiting temperature rise of the two commercially available products was similar, and in the previous experiment, the effect was similar to that when TiO ₂ was added alone. Looking at the list of functional ingredients for the two products, Prototype 1 and Prototype 2, two chemical sunscreens that can block only UVA or UVB specific wavelength ranges, and a physical sunscreen that can block UV-visible/near-infrared light areas were used. ZnO In the case of TiO ₂ , since the refractive index is smaller than that of TiO ₂ and intensively blocks the UVA region, it was found that TiO ₂ plays a heat blocking role.

<Experimental Example 2. Cell death rate test according to irradiation time of near-infrared lamp>

MTT assay was performed on human dermal fibroblast cells to investigate cytotoxicity to near-infrared light. Fibroblast cells were thawed and used in the experiment after 1 to 2 passages in the medium. After culturing more than 70% of the cells in a cell culture dish, detach the cells with 1 ml of Trypsin/EDTA solution, aliquot 6 x 10 ⁴ cells/well into a 48 well plate, and then use 5% CO ₂ , 37 ℃ incubator. Incubated for 12 hours. After that, after irradiation of near-infrared rays for 10, 20, and 30 minutes each, 50 µl of MTT (3-(4,5)-dimethylthiahiazo-2-yl)-2,5-diphenyltetrazolium bromide) solution was injected and incubated for 2 hours and 30 minutes. I did. After removing the solution from the well plate, 500 µl of dimethyl sulfoxide (DMSO) was added and piped, and the absorbance was measured using an EpochTM microplate spectrophotometer (BioTek, USA).

Tetrazolium salt assay using MTT reagent is a method mainly used to measure changes in cell proliferation and apoptosis. When the cells are treated with yellow water-soluble MTT tetrazolium, the ring structure of tetrazolium is cut off by dehydrogenase present in the electron transport system in the mitochondria of living cells, and it is reduced to blue-violet non-water-soluble MTT formazan crystals. MTT formazan crystals are dissolved in dimethyl sulfoxide (DMSO) and absorbance is measured using an ELISA reader. At this time, the absorbance of formazan dye is around 540 nm.

The results of the cell death rate test according to the irradiation time of the near-infrared lamp are shown in FIG. 18. When the near-infrared rays were irradiated for 10 minutes and passed through the slide glass without cream, the survival rate was 80%, but in the case of TiO ₂ or DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%), the control and non-irradiation control and It was almost similar. When irradiated for 20 to 30 minutes, it can be seen that the cell viability increased more than that of Control. This result can be seen in the study that near-infrared rays activate cell regeneration ability. That is, when the near-infrared irradiation time is short, the cells are under stress and the survival rate of the cells decreases, but as the irradiation time increases, the cell regeneration capacity is activated, and it is judged that the survival rate of the cells increases when measured for 30 minutes.

<Experimental Example 3. Cell death degree observation using a fluorescence microscope>

Eclipse Ti-S (Nikon, Japan) fluorescence microscope was used to cross-verify the degree of human fibroblast cell death against near-infrared light. Live cells (using Calcein AM) and dead cells (using Propidium iodide (PI)) were observed through the double staining method.

35 × 10 mm 의 Cell culture dish에 25 × 10⁴ cells/dish의 세포를 분취한 후 5 % CO₂, 37 ℃에서 약 12시간 배양하였다. 암실조건에서 빛을 쬔 후 배지를 제거하고 Growth medium을 이용하여 2회 washing 후 Growth medium 1 ㎖와 working solution 0.5 ㎖를 섞어 15분 간 Incubation하여 관찰하였다.As for the fluorescent dye, 5 µl of 2 mM Calcein AM and 10 µl of PI were diluted in 5 ml of 10 mM PBS to prepare a working solution.

Cells of 25 × 10 ⁴ cells/dish were aliquoted into a 35 × 10 mm cell culture dish and incubated for 12 hours at 37° C. in 5% CO ₂ . After irradiating light in a dark room, the medium was removed, washed twice with a growth medium, mixed with 1 ml of the growth medium and 0.5 ml of the working solution, and incubated for 15 minutes to observe.

At this time, after placing the cells cultured for more than 12 hours under the near-infrared lamp, the intensity of the lamp was fixed. The distance between the bottom of the cell culture dish and the lamp is 25 cm, and the light of the lamp is 15 cm away from the lamp. After passing through the slide glass, it was allowed to reach the cell. After applying various cream samples to the slide glass, light was applied every 10 minutes, 20 minutes, and 30 minutes, and then observed with a fluorescence microscope through the above method.

Each result is shown in Figs. 18 to 20. In each figure, Calcein AM and Propidium iodide (PI) were used, and Calcein AM penetrates the cell membrane and is hydrolyzed by the intracellular Esterase enzyme, converted to Calcein, and has green fluorescence, meaning a living cell. PI does not penetrate the cell membrane and intervenes in the DNA sequence that is eluted when the cell wall is destroyed, and shows red fluorescence, meaning dead cells.

[Fig. 18] is a result of observing cells with a bright field image after irradiation with near-infrared rays for 10 minutes and measuring Calcein and PI fluorescence. When only the slide glass is transmitted, a little PI fluorescence is seen, but other samples or near-infrared rays are not irradiated. There was no difference from the control that was not.

[Fig. 19] and [Fig. 20] are images observed with a fluorescence microscope after irradiation of near-infrared rays for 20 minutes and 30 minutes, respectively. PI is generated slightly more than when measured for 10 minutes, but there is no significant difference. Therefore, it can be seen that the near-infrared rays themselves do not affect cell death.

<Experimental Example 4. Reactive Oxygen Species (ROS) Measurement>

After applying the cream to the slide glass, use the reactive oxygen reactive dye 2',7'- Dichlorofluorescein diacetate (DCF-DA) to determine the degree of occurrence of Reactive Oxygen Species (ROS) in human fibroblast cells by transmitted light. Dyed. The stained cells were observed for Green fluorescence of DCF-DA using a fluorescence microscope.

35 × 10 mm 의 Cell culture dish에 25 × 10⁴ cells/dish의 세포를 분취한 후 5 % CO₂, 37 ℃에서 약 12시간 배양하였다. 암실조건에서 빛을 쬔 후 배지를 제거하고 working solution을 1.5 ㎖ 넣어 30분 간 Incubation하였다. Incubation이 끝나면 suction후 5 % FBS가 포함된 PBS 용액을 이용해 1회 washing 후 1㎖를 넣어 형광현미경으로 관찰하였다.After diluting DCF-DA to 25 μM in the growth medium, it was used as a working solution.

Cells of 25 × 10 ⁴ cells/dish were aliquoted into a 35 × 10 mm cell culture dish and incubated for 12 hours at 37° C. in 5% CO ₂ . After light was applied in dark conditions, the medium was removed, 1.5 ml of the working solution was added, and incubated for 30 minutes. After incubation, after suction, 1 ml of PBS containing 5% FBS was used, and then 1 ml was added and observed with a fluorescence microscope.

After installing a near-infrared lamp and placing the cell 25 cm below it, the light was exposed every 10 minutes, 20 minutes, and 30 minutes. After applying various cream samples to the slide glass, active oxygen generated by light reaching the cell was observed with a fluorescence microscope through the above method.

In the case of near-infrared rays, it penetrates deeply into the skin, exerts heat stress on cells, and accordingly, ROS is released. ROS fluorescence was measured using a fluorescence microscope to determine the degree of ROS emission with the naked eye. DCF-DA, a reactive oxygen reactive dye, penetrates the cell membrane and is decomposed by Esterase in the cell, and when reacted with ROS, DCF with green fluorescence is formed.

The results for this are shown in [Fig. 21] to [Fig. 23].

[Fig. 21] is an image of observing the degree of ROS occurrence when the near-infrared irradiation time is 10 minutes, and there is no significant difference in the degree of ROS occurrence when the sample is applied and when the sample is not applied. [Fig. 23] and [Fig. 24] are images that observe the degree of ROS generation when the near-infrared rays are irradiated for 20 minutes and 30 minutes, respectively. As the irradiation time increases, the amount of ROS generation increases and DGT (TiO ₂ 10 wt% + When the slide glass coated with DGNR 0.03 wt%) was passed through, the amount of ROS generated was significantly less compared to that when only the slide of the untreated group was passed through. This suggests that the DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%) cream prepared in the present invention effectively blocks near-infrared rays.

<Experimental Example 5. Human Matrix Metalloproteinase-1 expression level measurement>

A Cell-based ELISA kit using an antigen-antibody reaction was used to measure the expression level of Human Matrix Metalloproteinase-1 (MMP-1) in human dermal fibroblast cells by light transmitted through the cream-coated slide glass.

96-well plate in ^{Fibroblast cell 2 × 10 4 cells /} well and then separated one by one into the Growth medium was 200 ㎕ cultured for about 12 hours in 5% CO _2, 37 ℃. After irradiating light in a dark room condition, the medium was removed and rinsed twice using 1× TBS solution. 100 µl of the fixing solution was added and incubated at room temperature for 20 minutes. After removing the fixing solution, it was washed with 1× Wash buffer, and 100 µl of quenching buffer was added and incubated for 20 minutes at room temperature. After washing three times using 1× Wash buffer, 200 µl of Blocking Buffer was added and incubated for 1 hour at room temperature. After washing with 1× Wash buffer, 50 μl of 1× Primary antibody (Anti-MMP-1 Antibody) was added and incubated at 4° C. for 16 hours. After 16 hours, washing with 1× Wash buffer, 50 μl of 1× Secondary antibody (HRP-Conjugated Anti-Rabbit IgG Antibody) was added, and incubated at room temperature for 1 hour and 30 minutes using an orbital shaker. After washing with 1× Wash buffer, 50 µl of Ready-to-Use substrate was added and incubated for 30 minutes in a dark room at room temperature. Then, 50 µl of Stop solution was added and the absorbance at 450 nm was measured using an ELISA reader.

Next, the experiment was conducted by installing a near-infrared lamp. After applying various cream samples to the slide glass, the amount of expression of MMP-1 generated by the light reaching the cell was measured for 30 minutes by using an ELISA reader and compared with the control without irradiation of light.

It is known that when UV is irradiated on the skin, Matrix metalloproteinase-1 (MMP-1), a protein that decomposes collagen present in the dermal layer, occurs. Similar to UV irradiation, when IR-A (NIR) is irradiated, singlet oxygen (ROS) is generated and ERK1/2 is activated as a catalyst, thereby promoting the expression of MMP-1 protein.

[Fig. 24] is a result of measuring the expression level of MMP-1 after culturing 2 × 10 ⁴ cells/well fibroblast cells in a 96-well plate and irradiating near infrared for 30 minutes so that ROS is sufficiently expressed. When the slide glass and vehicle were penetrated based on the control, the amount of MMP-1 expression increased by about 2 times. At this time, as the temperature increase inhibition rate (TRI) increased, the expression level of MMP-1 decreased, and in the case of DGT (TiO ₂ 10 wt% + DGNR 0.03 wt%), it can be seen that the expression level almost similar to that of Control.

<Cosmetic formulation example 1. Preparation of flexible lotion-1>

As shown in the composition of Table 4 below, a dog bone gold nanorod and a flexible lotion (skin, 100g) containing titanium dioxide were prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) hardened castor oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 2. Preparation of nutrient lotion>

A nutrient lotion (lotion, 100 g) containing dog bone gold nanorods and titanium dioxide as shown in Table 5 was prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 3. Preparation of nutritional cream>

As shown in the composition of Table 6 below, a nutrient cream (100g) containing dog bone gold nanorods and titanium dioxide was prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 Sitosterol 4.0 Polyglyceryl 2-oleate 3.0 Ceramide 0.7 Ceteares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic Formulation Example 4. Preparation of Essence>

As shown in the composition of Table 7 below, an essence (100g) containing dogbone gold nanorods and titanium dioxide was prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 5. Preparation of foundation>

As shown in the composition of Table 8 below, a foundation (100g) containing dogbone gold nanorods and titanium dioxide was prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 Beeswax 2.0 Cyclomethicone 2.0 Floating paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic glycerin monostearate 3.0 Caprylic/Capric Triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butylene glycol 3.0 Triethanolamine 1.0 Aluminum Magnesium Silicate 0.5 Pigment 12.0 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 6. Preparation of hair shampoo>

As shown in the composition of Table 9 below, a hair shampoo (100g) containing dogbone gold nanorods and titanium dioxide was prepared according to a conventional method.

Raw material Content (g) Dogbone Gold Nanorod 0.03 Titanium dioxide 10.0 Arachidyl glucoside 4.5 ethanol 2.0 Butylene glycol 2.0 Citric acid 0.1 Phenoxyethanol 0.02 Purified water Balance

Claims (5)

Dog bone gold nano rod (Dog bone Gold Nano Rod) 0.015 ~ 0.03 wt% and titanium dioxide 5 ~ 10 wt% A cosmetic composition for near-infrared and heat shielding, characterized in that it contains. The method of claim 1,
The gold nanorod is a cosmetic composition for near-infrared rays and heat shielding, characterized in that the gold nanorods in the form of a rod or a dog bone. The method of claim 1,
A cosmetic composition for near-infrared and heat shielding, characterized in that the length of the gold nanorod is 30 ~ 1000nm. The method of claim 1,
The composition is a near-infrared and heat-blocking cosmetic composition, characterized in that it blocks a light source of a wavelength of 700 ~ 1,200nm near-infrared region. The method of claim 1,
The cosmetic composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing A cosmetic composition for near-infrared and heat shielding, characterized in that it has a formulation selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

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