KR102117651B1 - Oriental medicine composition for anti-oxidation, anti-wrinkle or anti-inflammation and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to an anti-oxidation, anti-wrinkle or anti-inflammatory herbal composition and a cosmetic composition comprising the same. The present invention provides an herbal composition having an antioxidant, anti-wrinkle or inflammation-improving effect, and a cosmetic composition comprising the same, including extracts of ginseng pear, saffron, youngsil, ginseng, licorice, zinnia, Angelica, hwangnyeon, and eoseongcho.

Description

Antioxidant, anti-wrinkle or anti-inflammatory herbal composition and cosmetic composition comprising the same{ORIENTAL MEDICINE COMPOSITION FOR ANTI-OXIDATION, ANTI-WRINKLE OR ANTI-INFLAMMATION AND COSMETIC COMPOSITION COMPRISING THE SAME}

The present invention relates to a cosmetic composition comprising an antioxidant, anti-wrinkle or anti-inflammatory herbal composition and an anti-oxidation, anti-wrinkle or anti-inflammatory herbal composition.

Free radicals produced during aerobic metabolic processes in the body are transformed into free radicals to kill pathogens that have invaded the human body, but have high reactivity, causing oxidative damage to cells including proteins, lipid molecules, and DNA. There was. In particular, there is a problem in that secondary diseases such as cancer, anemia, myocardial infarction, cerebral palsy, and atherosclerosis occur due to oxidative damage to these cells.

In addition, various types of inflammatory reactions may occur due to oxidative damage to cells caused by free radicals and external stimuli. Inflammatory reactions include reactive oxygen species, including reactive oxygen, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and PEG ₂ (Pro-inflammatory cytokine). Cytokine is involved. When the cytokine and the active nitrogen species are overexpressed, not only causes tissue damage of various organs and body organs, but also nerve deterioration, edema, itchiness, inflammation, bleeding, high fever, etc., are further deteriorated.

Accordingly, synthetic antioxidants such as Butylated hydroxyl anisole (BHA), Butylated hydroxyl toluene (BHT), Nrdihydroguaiaretic acid (NDGA), and nonsteroidal anti-inflammatory drugs such as fluphenamic acid, ibuprofen, and indomethacin, prednisolone, and dexamethasone Mainly used. However, the antioxidants and anti-inflammatory agents as described above cause allergies, and the use amount is limited due to safety problems for the skin or the body, so there is a problem that the effect is not large.

On the other hand, collagen is one of the skin components that play the role of mechanical firmness of skin, resistance of connective tissue, and support of cell adhesion, and is closely related to skin wrinkles. In addition, elastin (Elastin) fiber is a material that prevents the formation of wrinkles on the skin by forming cross-links with collagen. These collagen and elastin are degraded by the degrading enzymes collagenase and elastase, causing skin wrinkles and skin aging, and various attempts to inhibit the activity of the degrading enzyme while promoting the production of collagen and elastin there was. For example, retinol, retinoid, adenosine, animal placenta-derived proteins, and the like are used.

However, retinol, retinoids, etc. are substances that promote collagen synthesis and inhibit elastase enzyme activity, but are unstable and irritating to the skin. There was a problem that was not economical. Accordingly, it is made of an extract of natural ingredients that are safe for the human body, but there is a need to develop a material having excellent antioxidant, anti-wrinkle and anti-inflammatory effects.

Republic of Korea Registered Patent No. 10-0783626 Republic of Korea Patent Publication No. 10-2015-0102058 Republic of Korea Registered Patent No. 10-1796462 Republic of Korea Registered Patent No. 10-1846773

The first object of the present invention is to provide an herbal composition having antioxidant, anti-wrinkle or inflammation-improving effects, including extracts of ginseng, saffron, youngsil, ginseng, licorice, huanghuang, Angelica, hwangnyeon, and eoseongcho. .

The second object of the present invention includes an herbal composition comprising extracts of ginseng, saffron, Yeongsil, Ginseng, Licorice, Chihwang, Angelica, Hwangnyeon, and Eoseongcho as effective compositions, and thus has antioxidant, anti-wrinkle, or inflammation-improving effects. It is to provide a cosmetic composition.

The object of the present invention is not limited to the technical problems as described above, and another technical problem may be derived from the following description.

In order to achieve the first object, the present invention provides an herbal composition for antioxidant, wrinkle improvement, or inflammation improvement including extracts of ginseng, saffron, youngsil, ginseng, licorice, huang, Angelica, Hwangnyeon and Eoseongcho.

The herbal composition is 1 to 5: 0.5 ~ 2.5: 0.1 ~ 1: 0.1 ~ 1: 0.5 ~ 2: 0.1 ~ 1: 0.1 ~ The five ginseng, saffron, Yeongsil, Ginseng, Licorice, Chihwang, Angelica, Hwangnyeon and Eoseongcho 1: 0.1 to 1: 0.5 to 2 may be included in a weight ratio.

The herbal composition may include the ginseng, saffron, Yeongsil, ginseng, licorice, geohwang, Angelica, Hwangnyeon, and Eoseongcho in a weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1.

The extract may be extracted with one or more extraction solvents selected from the group consisting of water, methanol, ethanol, propanol, butanol, isopropanol, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol.

In order to achieve the second object, the present invention is an antioxidant, wrinkle improvement or inflammation improvement comprising an herbal composition containing extracts of ginseng, saffron, yeongsil, ginseng, licorice, hwanghwang, Angelica, hwangnyeon, and eoseongcho as active ingredients Provide a cosmetic composition for.

The cosmetic composition may include the herbal composition in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is flexible cosmetic, nutrient cosmetic, convergent cosmetic, skin, lotion, essence, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream, cleansing water, shampoo, rinse, conditioner , Hair gel, hair serum, hair essence, body lotion, body wash and body spray may be one or more formulations selected from the group consisting of.

The herbal composition of the present invention may have antioxidant, anti-wrinkle or inflammation-improving effects, including extracts of ginseng, saffron, youngsil, ginseng, licorice, rhizome, Angelica, hwangnyeon, and eoseongcho. In particular, the herbal composition of the present invention can realize the antioxidant, anti-wrinkle and inflammation-improving effects at the same time by including the ginseng, saffron, Yeongsil, ginseng, licorice, hwanghwang, Angelica, Hwangnyeon, and Eoseongcho in a specific ratio.

In more detail, the herbal composition of the present invention can have an antioxidant effect by increasing the content of polyphenols and scavenging DPPH radicals, ABTS radicals, and superoxide anion radicals. The herbal composition of the present invention can improve wrinkles by inhibiting the activity of elastase and inhibiting the activity of collagenase. That is, the anti-wrinkle effect can be realized. The herbal composition of the present invention has little cytotoxicity, inhibits the production of nitric oxide (NO), and inhibits the production of pro-inflammatory cytokine, thereby improving inflammation.

In addition, the cosmetic composition of the present invention, as the composition comprises an herbal composition containing extracts of ginseng, saffron, Yeongsil, ginseng, licorice, huang, Angelica, Hwangnyeon and Eoseongcho as an active ingredient, antioxidant, wrinkle improvement or inflammation improvement It can have an effect.

1 is a view showing the experimental results of evaluating the DPPH radical scavenging ability of Examples 1 to 4 in Experimental Example 2.
2 is a view showing the experimental results of evaluating the ABTS radical scavenging ability of Examples 1 to 4 in Experimental Example 2.
FIG. 3 is a view showing the results of experiments in which the superoxide anion radical scavenging ability of Examples 1 to 4 in Experimental Example 2 was evaluated.
4 is a diagram showing the results of experiments in which the elastase activity inhibitory activity of Examples 1 to 4 in Experimental Example 3 was evaluated.
FIG. 5 is a graph showing the results of experiments in which collagenase activity inhibition of Examples 1 to 4 in Experimental Example 3 was evaluated.
FIG. 6 is a diagram showing the results of experiments in which the cell viability (%) of Example 2 in Experimental Example 4 was evaluated.
7 is a view showing an experimental result of evaluating the NO production amount (%) of Example 2 in Experimental Example 4.
FIG. 8 is a diagram showing the results of experiments in which the amount of Pro-inflammatory cytokine production (%) of Example 2 in Experimental Example 4 was evaluated.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains may easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. In addition, parts not related to the description are omitted in the drawings to clearly describe the present invention.

The terms or words used in the specification and claims of the present invention are not to be construed as being limited in a conventional or lexical sense, and the inventor can appropriately define the concept of terms to describe his or her invention in the best way. Based on the principles, it should be interpreted as meanings and concepts consistent with the technical spirit of the present invention.

Throughout the specification of the present invention, when a part is to “include” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated. .

Throughout the specification of the present invention, "A and/or B" means A or B, or A and B.

The present invention provides an herbal composition for anti-oxidation, wrinkle improvement or inflammation improvement.

In one embodiment of the present invention, according to including extracts of baejaja, saffron, Yeongsil, ginseng, licorice, geohwang, Angelica, hwangnyeon and Eoseongcho, it provides an herbal composition for antioxidant, wrinkle improvement or inflammation improvement. In particular, the herbal composition of the present embodiment may include anti-oxidant, wrinkle-improving and inflammation-improving effects at the same time by including a specific ratio of ginseng, saffron, youngsil, ginseng, licorice, hwanghwang, Angelica, hwangnyeon, and eoseongcho.

The'extract' used in the present embodiment is an extract obtained by the extraction treatment of ginseng, saffron, youngsil, ginseng, licorice, huang, Angelica, Hwangnyeon and Eoseongcho, a diluent or concentrate of the extract, and a dried product obtained by drying the extract , And extracts of all formulations that can be formed using the extract itself and the extract, such as a crude or purified product of the extract. The extract of the present invention may be preferably used in a dry powder form after extraction.

Gallnut (Aphis chinensis) is a sleeping bag (worm pouch) parasitic on Rhus javanica Linne of the lacquer family. The constellation is uneven and irregularly divided into 2 or 4 pouch-shaped or broken. Ojaja contains tannin as a main component, so it exhibits a branching action and an antibacterial action by converging action.

In general, high quality tannins with high tannin content can be obtained by collecting them in early autumn and then drying them with steam. In oriental medicine, it is mainly used for diarrhea, colitis, dysentery, gastrointestinal bleeding, hemorrhage, nosebleeds, cold sweat, and sweating. It is also used for traumatic bleeding, inflammation of mucous membranes, ulcers, and eczema.

Lespedeza bicolor is a perennial deciduous tree that is about 3 m high and has dark brown ridges, and the wood is light green. In general, it blooms between July and August, and the fruit matures in October and is a plant distributed in Korea, China, Japan, and Far East Russia.

In Chinese medicine, the stem and roots of the stalk tree are called gyrus, and are used for the treatment of Jinhae, Geodam, chronic bronchitis, hemostasis, blue fever, astringent, sweating, antipyretic, diuretic, and dry stool. Quercetin, Isoquercitrin, Orientin, Trifolin, Kaemferol, etc. are the main components of fern tree.

Rosa multiflora fruit is a dried medicinal herb from the rose multiflora Thunberg. It is commonly grown in wild mountains in Korea.It is mainly used in the treatment or improvement of cold, flu, degenerative and rheumatoid arthritis in Korea, Japan and China. Has been used. Yeongsil is spherical, elliptical, and round, and the outer surface is glossy with red or dark reddish brown, and there are residues of pentagonal pistil heads at the end of the fruit. On the inner wall that cut Yeongsil horizontally, silver-white hairs are dense and contain 5 to 20 hard seeds.

Quercetin glycosides including flavonoids, Multinoside A, Multinoside B, and quercitrin; Kaempferol glycosides including Multiflorin A, Multiflorin B, Afzelin, Astragalin and the like; Tannin-based methyl gallate; Lycopene, a reddish tint, is mainly included.

Ginseng (Panax ginseng C. A. Meyer) is a perennial herbaceous plant belonging to the family Elmaceae. The root is called Ginseng Radix in oriental medicine, and saponin is a typical ingredient. Ginseng saponins are called ginsenosides in the sense of glycosides to distinguish them from other plant-based saponins. Most pharmacological action is by ginsenoside, and ginsenoside can be classified into Oleanane, Panaxadiol, Panaxatriol, etc. It is effective for anti-cancer, anti-inflammatory, and strengthening immunity.

Licorice (Glycyrrhiza　uralensis) is a medicinal plant that is a perennial plant of the Rosaceae family of dicotyledonous plants. Mainly, the root is used as a medicinal material, but the root has a sweet taste and is non-toxic and has a warm energy. The main component of licorice is Glycyrrhizin, a triterpenoid-based saponin, containing about 3 to 5% in the roots, anti-inflammatory, anti-allergic, antioxidant, anti-ulcer, anti-viral and anti-cancer It has the same physiological activity characteristics. In addition, Liqiuritin, Liquiritigenin, and Isoliquiritigenin are included as main ingredients. Such licorice has a high sweetness and has the ability to improve foam, emulsification, and flavor, and is mainly used in the food, pharmaceutical, and cosmetic industries.

Jihwang (Rehmannia glutinosa) is a perennial plant belonging to the present ginseng family and is a medicinal plant native to China. The leaves of the rhubarb are long oval, have many wrinkles, and have soft white hairs all over the body. In addition, the roots of qi hwang are used as herbal medicines, and can be divided into raw gin hwang, geonji hwang, and sukjihwang depending on the purpose.

The main components of sulfur are phytosterols, saccharides, iridoid glycosides, inorganic elements, chrysoeriol, luteolin and 11 amino acids. . Raw and dried sulfur are beta-sitosterol, Stigmasterol, Campesterol, Rehmanin, Fatty acids, Catalpol, Glucose, γ- It contains ingredients such as butyl-amino acid, carbohydrate, norcarotenoid, stachyose, and arginine, while succinate sulfur is stachyose and verabas Sugars such as Verbascose, Mannotriose, Raffinose, Sugar, Glucose, Fructose, Galactose, Catalpol, etc. It has been reported that vitamin A, Arginine, Mannitol, and β-sitosterol are contained in small amounts. The main efficacy is blood, tonic antipyretic, especially anemia, hemolysis, or tuberculosis.

Angelica sinensis (Angelica sinensis) is a perennial aromatic herb belonging to the apiaceae family, and the roots of 1~2 year-old Angelica are collected in the fall and used mainly for drying. The physiologically active ingredients of Angelica are Pyranocoumarin compounds Decursin, Decursinol and Nodakenin, and volatile scent components alpha-pinene and beta-cucumber Desmol (β-eudesmol), phenol (Phenol), O-cresol (O-cresol), P-cresol (P-cresol), guaiacol (Guaiacol), p-methybenzolcohol, p-ethylphenol (p-ethylphenol) ), o-ethylphenol, 2,3-Dieresol, Carvacrol, Isoeugenol, Vanillin, and the like. Mainly, it has the effect of preventing skin aging, removing fine lines, detoxification, promoting blood circulation, preventing constipation, and forming hematopoiesis.

Cloveris (Coptis chinensis Franch) is a perennial plant belonging to the family Asteraceae, originating from China and cultivated in Korea, Japan, and China for medicinal purposes. In Oriental medicine, roots are collected and dried in the sun. They have a peculiar smell, bitter taste, and cold properties. Isoquinoline Alkaloids include Berberine, Jateorrhizine, Palmatine, Coptisine, Magnoflorine, Epiberberin, Berbestine, Warrenine and Ferulic Acid (Ferulic acid). Berberine, a major component of Hwangnyeon, is known to have excellent antibacterial effects and anti-inflammatory, hemostatic, lowering blood pressure, and anticancer effects.

Eoseongcho (Houttuynia cordata) is a perennial herb belonging to the genus Sambaek, and is the outpost of medicinal wheat. The name Eoseongcho is named because it smells fishy from the stems and leaves. It has a stronger smell from fresh ones. In Korea, it is grown or cultivated in the central region. Eoseongcho is known to contain large amounts of quercetin, hyperin, flavonoid, alkaloid, etc., and has antibacterial, immune-enhancing, anti-inflammatory, antiviral, anti-leukemia, anti-cancer, etc. It works.

The extract from the herbal composition of the present embodiment is 5 ~ 0.5: 2.5 ~ 2.5: 0.1 ~ 1: 0.1 ~ 1: 5 ~ 0.5: 2.5 ~ 0.1: 1 ~ 0.1 ~ 1: baejaja, saffron, Yeongsil, ginseng, licorice, Chihwang, Angelica, Hwangnyeon and Eoseongcho : 0.1 ~ 1: 0.1 ~ 1: As included in a weight ratio of 0.5 to 2, it may have an antioxidant, wrinkle improvement or inflammation improvement effect. Preferably, the herbal composition of the present embodiment comprises the weight ratio of 4: 1: 0.5: 0.5: 1.5: 0.5: 0.5: 1 to include the ginseng, saffron, Yeongsil, Ginseng, Licorice, Chihwang, Angelica, Hwangnyeon and Eoseongcho. When doing, it can have more excellent antioxidant, wrinkle improvement or inflammation improvement effect.

According to the present embodiment, in the herbal composition comprising extracts of ginseng, pear tree, youngsil, ginseng, licorice, turmeric, Angelica, hwangnyeon and Eoseongcho, the extraction solvent of the extract is water, methanol, ethanol, propanol, butanol, isopropanol, It may be one or more selected from the group consisting of acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. Preferably, the extraction solvent of this embodiment may be ethanol, but is not limited thereto.

The herbal composition of the present embodiment may have an antioxidant effect by increasing the content of polyphenols and scavenging DPPH radicals, ABTS radicals, and superoxide anion radicals.

The herbal composition of the present embodiment can improve wrinkles by inhibiting the activity of elastase and inhibiting the activity of collagenase. That is, the anti-wrinkle effect can be realized.

The herbal composition of this embodiment has little cytotoxicity, inhibits the production of nitric oxide (NO), and inhibits the production of pro-inflammatory cytokine, thereby improving inflammation.

In addition, the herbal composition of the present embodiment can have anti-oxidation, anti-wrinkle or anti-inflammatory effects, thereby preventing aging.

The herbal composition of the present embodiment may be applied to a cosmetic composition, a food composition, a health food composition, a pharmaceutical composition, and the like.

In addition, the present invention provides a cosmetic composition comprising an herbal composition for antioxidant, wrinkle improvement or inflammation improvement.

In one embodiment of the present invention, by containing the herbal composition containing the extract of baejaja, pear tree, Yeongsil, ginseng, licorice, geohwang, Angelica, hwangnyeon and Eoseongcho as active ingredients, it has an antioxidant, wrinkle improvement or inflammation improvement effect Provided is a cosmetic composition. In particular, the herbal composition of the present embodiment may include anti-oxidant, wrinkle-improving and inflammation-improving effects at the same time by including a specific ratio of ginseng, saffron, youngsil, ginseng, licorice, hwanghwang, Angelica, hwangnyeon, and eoseongcho.

The cosmetic composition of the present embodiment may include the herbal composition in an amount of 0.01 to 10% by weight, preferably 0.1 to 7% by weight, and more preferably 1 to 5% by weight, based on the total weight of the cosmetic composition, but is not limited thereto. .

If, when the cosmetic composition of the present embodiment contains less than 0.01% by weight of the herbal composition based on the total weight, there may be a slight problem of anti-oxidation, wrinkle improvement or inflammation improvement effect. On the other hand, it is not economical to include the herbal composition in excess of 10% by weight based on the total weight of the cosmetic composition of the present embodiment, there may be a problem that the formulation stability of the cosmetic composition is lowered.

The cosmetic composition of the present embodiment includes an herbal composition comprising extracts of ginseng, saffron, youngsil, ginseng, licorice, huanghuang, dong quai, hwangnyeon, and eoseongcho as active ingredients, along with dermatologically acceptable excipients, softening makeup, Nourishing lotion, converging lotion, skin, lotion, essence, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream, cleansing water, shampoo, conditioner, conditioner, hair gel, hair serum, Hair essence, body lotion, body wash and may be formed of one or more formulations selected from the group consisting of body spray, but is not limited thereto.

The excipients include skin softeners, skin penetration enhancers, colorants, fragrances, emulsifiers, thickeners, solvents, fragrances, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, synthetic polymers, chelating agents, proteins, minerals , Vitamins, and the like, but are not limited thereto, and may include all known excipients.

The cosmetic composition for anti-oxidation, wrinkle improvement or inflammation improvement of the present invention includes all of the contents of the herbal composition for anti-oxidation, wrinkle improvement or inflammation improvement of the present invention.

Through the following examples, comparative examples, and experimental examples will be described in detail for the antioxidant composition, wrinkle improvement or inflammation improvement of the present invention and cosmetic compositions comprising the same. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

[Example]

Examples 1 to 4

A dried sample was prepared by mixing the ginseng, sage, Yeongsil, ginseng, licorice, Chihwang, Angelica, Hwangnyeon, and Eoseongcho according to the ratio described in Table 1 [unit: weight ratio]. At this time, the total amount of dry samples in Examples 1, 2, 3 and 4 were all the same.

Subsequently, ethanol (70%) 10 times the weight of the dry sample was added, followed by immersing at room temperature for 24 hours to extract for 3 times. After extracting and concentrating each extract, freeze-drying (-20 ~ 0 ℃), an extract was prepared.

Example 1 Example 2 Example 3 Example 4 gallnut 1.5 4 2 3 Cypress 1.5 One 2 One Yeongsil One 0.5 One 0.5 Ginseng One 0.5 0.5 0.5 licorice One 1.5 One One Hell One 0.5 One One Angelica One 0.5 One One goldthread One 0.5 0.5 One Eoseongcho One One One One

[Comparative Example]

Comparative Examples 1 to 9

An extract was prepared in the same manner as in Example 1, except that a dry sample mixed in a weight ratio of Table 2 [unit: weight ratio] was used.

Comparative example One 2 3 4 5 6 7 8 9 gallnut - 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cypress 1.5 - 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Yeongsil One One - One One One One One One Ginseng One One One - One One One One One licorice One One One One - One One One One Hell One One One One One - One One One Angelica One One One One One One - One One goldthread One One One One One One One - One Eoseongcho One One One One One One One One -

[Experimental Example]

Experimental Example 1: Polyphenol content increase evaluation

To quantify polyphenols, 1 mL of 1N-Folin-Ciocalteu phenol reagent is added to 3 mL of a 100-fold diluted sample solution (Example or Comparative Example), 0.2 mL of 1N HCl is added, and then 1 mL of saturated Na ₂ CO ₃ solution After adding and mixing, it was left to stand for about 1 hour at room temperature, the absorbance was measured at 640 nm, and the polyphenol content [mg/g] was calculated by comparing the absorbance value of a standard curve prepared in advance with tannic acid, a standard material. , The results are shown in Table 3.

Total polyphenol
(mg TAE/g 1) Total polyphenol
(mg TAE/g 1) Example 1 49.3 Comparative Example 3 27.4 Example 2 75.2 Comparative Example 4 19.3 Example 3 58.5 Comparative Example 5 22.4 Example 4 67.1 Comparative Example 6 21.8 Comparative Example 1 23.0 Comparative Example 7 19.2 Comparative Example 2 22.1 Comparative Example 8 20.9 Comparative Example 9 23.5

Looking at Table 3, according to this embodiment, when including all of baejaja, saffron, youngsil, ginseng, licorice, hwanghwang, Angelica, hwangnyeon, and eoseongcho, it can be seen that the total polyphenol content increases. In particular, when the weight ratio of 4: 1: 0.5: 0.5: 1.5: 0.5: 0.5: 0.5: 1 is included, the total polyphenol content of quince, saffron, youngsil, ginseng, licorice, hwang, Angelica, hwangnyeon, and eoseongcho It can be seen that it is significantly increased.

Experimental Example 2: antioxidant evaluation

(1) DPPH radical scavenging ability

DPPH electron donating ability (EDA) was measured as follows.

1 mL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 mL of each sample solution, stirred, and allowed to stand for 30 minutes, and absorbance was measured at 517 nm. The electron donating ability was represented by the absorbance reduction rate of the sample solution added group and the non-added group.

Inhibition rate% = [1-(absorbance of the sample-added group / absorbance of the sample-free group)] X 100

According to the above, it was shown in Figure 1 to evaluate the DPPH radical scavenging ability of Examples 1 to 4. Particularly, when the treatment concentration is 1,000 μg/ml, the DPPH radical scavenging ability of Examples and Comparative Examples is shown in Table 4.

At this time, in Figures 1 to 8, HC-1 means Example 1, HC-2 means Example 2, HC-3 means Example 3 and HC-4 means Example 4, and vit-C uses vitamin C. Means

DPPH radical scavenging ability (%) DPPH radical scavenging ability (%) Example 1 73.8 Comparative Example 3 40.2 Example 2 93.1 Comparative Example 4 38.4 Example 3 86.7 Comparative Example 5 35.0 Example 4 78.7 Comparative Example 6 41.1 Comparative Example 1 42.3 Comparative Example 7 37.6 Comparative Example 2 38.5 Comparative Example 8 35.7 Comparative Example 9 41.9

(2) ABTS radical scavenging ability

7 mM ABTS buffer and 2.45 mM potassium persulfate buffer (K ₂ S ₂ O ₈ ) were prepared, ABTS and K ₂ S ₂ O ₈ were mixed at a ratio of 2: 1, and then reacted by blocking for 12 to 16 hours. The stock buffer was diluted so that the OD value measured at 734 nm was 0.70±0.02, and 100 μL of the extract sample solution diluted by concentration was added to 100 μL of the buffer, and then reacted at room temperature for 6 minutes, and absorbance was measured at 734 nm. Thus, the absorbance of the added and non-added groups was shown as a decrease rate.

Inhibition rate% = [1-(absorbance of the sample-added group / absorbance of the sample-free group)] X 100

According to the above, the ABTS radical scavenging ability of Examples 1 to 4 was evaluated and shown in FIG. 2. Particularly, when the treatment concentration is 10 μg/ml, ABTS radical scavenging ability of Examples and Comparative Examples is shown in Table 5.

ABTS radical scavenging capacity (%) ABTS radical scavenging capacity (%) Example 1 36.4 Comparative Example 3 11.8 Example 2 93.6 Comparative Example 4 15.1 Example 3 49.4 Comparative Example 5 12.9 Example 4 23.4 Comparative Example 6 13.3 Comparative Example 1 12.3 Comparative Example 7 13.0 Comparative Example 2 13.4 Comparative Example 8 12.5 Comparative Example 9 12.4

(3) Superoxide anion radical scavenging ability

Superoxide anion radical scavenging ability was measured by nitrobule tetrazolium (NBT) reduction method.

0.1 mL of each sample solution and 0.4 mL of 0.1 M potassium phosphate buffer (pH 7.5) were added 1 mL of substrate solution in which Xanthine (0.4 mM) and NBT (0.24 mM) were dissolved, and 1 mL of xanthine oxidase (0.2 U/mL). After the reaction was performed at 37°C for 20 minutes, the absorbance of the produced superoxide anion radical was measured at 560 nm.

Inhibition rate% = [1-(absorbance of the sample-added group / absorbance of the sample-free group)] X 100

According to the above, the superoxide anion radical scavenging ability of Examples 1 to 4 was evaluated and shown in FIG. 3. Particularly, when the treatment concentration is 1,000 μg/ml, Superoxide anion radical scavenging ability of Examples and Comparative Examples is shown in Table 6.

Superoxide anion radical scavenging ability (%) Superoxide anion radical scavenging ability (%) Example 1 15.1 Comparative Example 3 5.8 Example 2 91.5 Comparative Example 4 5.9 Example 3 59.0 Comparative Example 5 8.1 Example 4 32.6 Comparative Example 6 7.2 Comparative Example 1 6.2 Comparative Example 7 7.7 Comparative Example 2 7.0 Comparative Example 8 6.4 Comparative Example 9 5.9

Looking at Tables 4 to 6, according to this embodiment, when including all of the ginseng, pear tree, youngsil, ginseng, licorice, sulfur, Angelica, hwangnyeon and Eoseongcho, DPPH radical, ABTS radical, peroxide anion radical scavenging ability is confirmed to be improved Can be. According to this embodiment, it can be confirmed that the DPPH radical, ABTS radical, and peroxide anion radical scavenging ability increase depending on the concentration.

In particular, when containing the weight ratio of baejaja, pear tree, youngsil, ginseng, licorice, geohwang, Angelica, hwangnyeon, and eoseongcho in the weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1, DPPH radical, ABTS radical , It can be seen that the peroxide anion radical scavenging ability is significantly improved.

That is, as the herbal composition of the present embodiment has excellent DPPH radical, ABTS radical, and peroxide anion radical scavenging ability, it means that the antioxidant effect is excellent.

Experimental Example 3: Wrinkle improvement evaluation

(1) Evaluation of Elastase Inhibition

Elastase inhibitory ability was measured using N-succinyl-(L-Ala)3-p-nitroanilide as a substrate, and the absorbance at 405 nm was measured for the amount of p-nitroanilide produced from the substrate at 37°C for 20 minutes.

0.5 mL of each test solution was taken to the test tube to a constant concentration, and 0.5 mL of a porcine pancreas elastase (2.5 U/mL) solution dissolved in 50 mM tris-HCl buffer (pH 8.6) was added, followed by 50 mM tris-HCl as a substrate. N-succinyl-(L-Ala)3-p-nitroanilide (0.5 mg/mL) dissolved in buffer (pH 8.6) was added, reacted for 20 minutes, and then measured. The elastase inhibitory ability was expressed by the rate of decrease in absorbance of the sample solution added group and the non-added group.

Inhibition rate% = [1-(absorbance of the sample-added group / absorbance of the sample-free group)] X 100

According to the above, it was shown in Figure 4 to evaluate the elastase inhibitory ability of Examples 1 to 4. Particularly, when the treatment concentration was 1,000 μg/ml, the inhibitory ability (%) of the elastase of Examples and Comparative Examples was shown in Table 7.

Elastase inhibitory activity (%) Elastase inhibitory activity (%) Example 1 8.82 Comparative Example 3 1.89 Example 2 40.72 Comparative Example 4 3.48 Example 3 25.1 Comparative Example 5 2.55 Example 4 21.2 Comparative Example 6 4.00 Comparative Example 1 2.35 Comparative Example 7 2.94 Comparative Example 2 3.05 Comparative Example 8 3.01 Comparative Example 9 2.39

Looking at Table 7, it can be seen that according to the present embodiment, when including all of ginseng, saffron, youngsil, ginseng, licorice, huanghwang, dong quai, hwangnyeon, and eoseongcho, the ability to inhibit elastase activity is improved. According to this embodiment, it can be confirmed that the inhibitory activity of elastase activity increases in a concentration-dependent manner.

Particularly, when baejaja, saffron, youngsil, ginseng, licorice, huanghuang, Angelica, hwangnyeon, and eoseongcho are included in a weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1, the ability to inhibit elastase activity It can be seen that this is significantly improved.

That is, it means that the herbal composition of the present embodiment has an anti-wrinkle (wrinkle improvement) effect by inhibiting the activity of elastase and preventing decomposition of elastin.

(2) Evaluation of collagenase inhibitory activity

To measure collagenase inhibitory activity, 4 mM CaCl ₂ was added to 0.1 M tris-HCl buffer (pH 7.5), and 0.25 mL of a substrate solution in which 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg/mL) was dissolved. And 0.15 mL of collagenase (0.2 mg/mL) was added to a mixture of 0.1 mL of the sample solution, allowed to stand at room temperature for 20 minutes, then 0.5 mL of 6% citric acid was added to stop the reaction, and 1.5 mL of ethyl acetate was added to add 320 nm. Absorbance was measured at. The ability to inhibit collagenase was expressed as the decrease in absorbance between the sample solution and the non-addition groups.

Inhibition rate% = [1-(absorbance of the sample-added group / absorbance of the sample-free group)] X 100

According to the above, it was shown in Figure 5 to evaluate the collagenase activity inhibitory ability of Examples 1 to 4. Particularly, when the treatment concentration is 1000 μg/ml, the inhibitory activity (%) of collagenase activity in Examples and Comparative Examples is shown in Table 8.

Collagenase
Active inhibitory activity (%) Collagenase
Active inhibitory activity (%) Example 1 82.2 Comparative Example 3 60.8 Example 2 97.1 Comparative Example 4 59.2 Example 3 86.2 Comparative Example 5 61.7 Example 4 94.9 Comparative Example 6 63.0 Comparative Example 1 62.4 Comparative Example 7 64.2 Comparative Example 2 58.9 Comparative Example 8 60.0 Comparative Example 9 59.7

Looking at Table 8, it can be seen that when including all of the ginseng, saffron, youngsil, ginseng, licorice, hwanghwang, Angelica, hwangnyeon, and eoseongcho, the inhibitory activity of collagenase activity is improved. According to this embodiment, it can be confirmed that the inhibitory activity of collagenase activity is increased in a concentration-dependent manner.

In particular, when including the ginseng, pear tree, Yeongsil, ginseng, licorice, geohwang, Angelica, hwangnyeon, and Eoseongcho in a weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1, it is possible to inhibit collagenase activity It can be seen that this is significantly improved.

That is, it means that the herbal composition of the present embodiment has an anti-wrinkle (wrinkle improvement) effect by inhibiting the activity of collagenase and preventing degradation of collagen.

Experimental Example 4: Inflammation improvement evaluation

(1) Cytotoxicity measurement

(a) Cell culture

For the culture of each cell, Dulbeco's modified eagle's medium (DMEM) medium to which 10% fetal bovine serum (FBS) was added was used, and the cells were subcultured by adapting to a 37°C, 5% CO ₂ incubator.

(b) MTT assay

For cell viability measurement, macrophage (Raw264.7) cells were seeded in a 96 well plate at 1×10 ⁶ cells/ml, and after 24 hours, a specific concentration (1, 5, 10, 50, 100, 500 μg/ml) After adding 20 μl of the sample, it was incubated for 24 hours in a 37°C, 5% CO ₂ incubator. The control group was cultured under the same conditions by adding the same amount of sterile water as the sample. After replacing with serum-free medium, 20 μl of MTT (5 mg/mL) solution was added and incubated for 4 hours, and then the culture solution was removed and 150 μl of DMSO: Ethanol (1: 1) per well was added and reacted at room temperature for 30 minutes. Then, absorbance was measured at 550 nm with an ELISA reader. The cell viability measurement was expressed as the absorbance reduction rate of the sample solution added group and the non-added group.

According to the above, it was shown in Figure 6 to evaluate the cytotoxicity of Example 2. Particularly, when the treatment concentration is 10 μg/ml, the cell viability (%) of Examples and Comparative Examples is shown in Table 9.

Cell viability (%) Cell viability (%) Example 1 78.4 Comparative Example 3 70.2 Example 2 90.2 Comparative Example 4 69.9 Example 3 82.6 Comparative Example 5 67.6 Example 4 83.1 Comparative Example 6 71.4 Comparative Example 1 65.6 Comparative Example 7 70.8 Comparative Example 2 67.8 Comparative Example 8 67.2 Comparative Example 9 69.8

(2) Nitrogen monoxide (NO) inhibitory evaluation

Nitric oxide (NO) was measured as the amount of NO in the supernatant of the cell as nitrite and nitrate. A safe form after reduction with nitrate for nitrite was used as a griess reagent (Sigma, USA). When 2×10 ⁶ cells in a 6 well plate were 80% confluenced, washed twice with PBS and serum-free medium Incubated for 12 hours or more using, and 10 μg/mL of lipopolysacchride (LPS) was added to all wells except the control group to stimulate. After 2 hours, the samples were treated by concentration and tested. The amount of NO was measured by absorbance at 540 nm after collecting supernatant after 24 hours and reacting with griess regent for 10 minutes.

According to the above, the amount of NO produced in Example 2 is shown in FIG. 7. Particularly, when the treatment concentration is 10 μg/ml, the inhibitory ability to produce NO in Examples and Comparative Examples (%) is shown in Table 10.

NO production inhibitory ability (%) NO production inhibitory ability (%) Example 1 48.2 Comparative Example 3 33.5 Example 2 61.4 Comparative Example 4 36.4 Example 3 50.1 Comparative Example 5 31.8 Example 4 50.8 Comparative Example 6 32.6 Comparative Example 1 32.4 Comparative Example 7 37.9 Comparative Example 2 38.9 Comparative Example 8 34.1 Comparative Example 9 36.0

(3) Evaluation of pro-inflammatory cytokine inhibitory ability

Raw 264.7 cells were adjusted to 2×10 ⁵ cells/mL, seeded in a 6-well plate, and cultured for 24 hours. Cells were cultured for 24 hours by simultaneously treating 1 μg/mL LPS and extract. The amount of PGE ₂ cytokine secreted in the cell culture was measured using an ELISA kit.

According to the above, the amount of Pro-inflammatory cytokine produced in Example 2 is shown in FIG. 8. Particularly, when the treatment concentration is 10 μg/ml, Pro-inflammatory cytokine inhibitory ability (%) of Examples and Comparative Examples is shown in Table 11.

On the other hand, in FIG. 8, the production amount is 69.2% at 5 μg/ml, and the production amount at 5 μg/ml is 55.7%.

Pro-inflammatory cytokine production inhibitory ability (%) Pro-inflammatory cytokine production inhibitory ability (%) Example 1 35.5 Comparative Example 3 22.4 Example 2 44.3 Comparative Example 4 18.6 Example 3 37.8 Comparative Example 5 23.1 Example 4 38.1 Comparative Example 6 22.4 Comparative Example 1 17.9 Comparative Example 7 19.5 Comparative Example 2 19.1 Comparative Example 8 21.8 Comparative Example 9 16.4

Looking at Tables 9 to 11, it can be seen that when including all of the ginseng, saffron, youngsil, ginseng, licorice, huang, Angelica, Hwangnyeon, and Eoseongcho, there is little cytotoxicity. In more detail, it can be confirmed that when the treatment concentration of Example 2 is 10 μg/ml, the cell survival rate is about 90% or more.

In addition, according to this embodiment, it can be confirmed that the concentration-dependent NO inhibitory capacity and Pro-inflammatory cytokine inhibitory capacity increase. In particular, as in Example 2, when including baejaja, pear tree, youngsil, ginseng, licorice, turmeric, Angelica, hwangnyeon and Eoseongcho in a weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1, It can be seen that the NO inhibitory ability and the pro-inflammatory cytokine inhibitory ability are significantly improved.

That is, the herbal composition of the present embodiment means that it has an effect of improving inflammation by inhibiting the activity of NO and Pro-inflammatory cytokine, which exacerbates inflammation when overexpressed.

[Formulation example]

Formulation Example 1: Preparation of flexible cosmetic water

The composition of the softening composition containing the herbal composition of the above example as an active ingredient is shown in Table 12 below.

Specifically, sodium hyaluronate was dispersed in purified water with a propeller mixer (3000 rpm) to prepare a 1% solution. Raw materials 1 to 8, except sodium hyaluronate, were homogenized at 500 rpm in a water phase melting tank using a propeller mixer, heated at 75°C to completely dissolve, and then cooled to room temperature. Subsequently, after completely dissolving the raw materials 9 to 11 in a separate dissolution tank, the mixture was added to the aqueous phase dissolution tank and stirred and mixed. Here, the herbal extract and persimmon extract of the Examples were added and thoroughly stirred and mixed to prepare softened cosmetic water. On the other hand, for the preparation, in addition to the ingredients disclosed in the table, it may further include known ingredients required for manufacturing.

Flexible cosmetic production
ingredient
Content (% by weight)
One
Herbal composition of Examples
0.01
2
Polyoxyethylene cured castor oil
0.5
3
Glycine
3.3
4
Dipotassium glyceride
0.1
5
1,3-butylene glycol
3.0
6
Sodium hyaluronate
0.1
7
ethanol
5.0
8
Antioxidant
0.1
9
Triethanolamine
0.1
10
EDTA
0.1
11
antiseptic
Proper
12
Purified water
Balance

Formulation Example 2. Preparation of nutrient makeup

The composition of the nutrient cosmetic composition containing the herbal composition of the above embodiment as an active ingredient is shown in Table 13 below.

Specifically, the carbomer was dispersed at 4000 rpm using a propeller mixer to prepare a 2% solution. After adding the raw materials 1 to 6 to the aqueous phase melting tank and stirring and dispersing with a homomixer (2000 rpm), the mixture was heated to 75°C. Raw materials 7 to 14 were added to the oil phase dissolving tank and heated and dissolved to 75°C. Then, the oil phase dissolved in the aqueous phase dissolution tank was added to emulsify (3000 rpm/5 minutes), and then cooled to room temperature. Here, the herbal composition of the Example was introduced and mixed thoroughly with stirring to prepare nutrient makeup. On the other hand, for the preparation, in addition to the ingredients disclosed in the table, it may further include known ingredients required for manufacturing.

Nutritional makeup
ingredient
Content (% by weight)
One
Herbal composition of Examples
0.01
2
glycerin
7.0
3
Sorbitan stearate sucrose cocoate
2.0
4
Mineral oil
4.0
5
Trioctanoin
1.0
6
Stearic Acid
1.0
7
Glyceryl stearate
0.5
8
Sorbitan monostearate
1.0
9
Dimethicone
0.5
10
Antioxidant
0.3
11
Triethanolamine
0.1
12
Carbomer
0.2
13
EDTA
0.1
14
antiseptic
Proper
15
Purified water
Balance

Formulation Example 3. Preparation of Essence

The composition of the essence containing the herbal composition of the above example as an active ingredient is shown in Table 14 below.

Specifically, sodium hyaluronate and hydroxyethyl cellulose were each dispersed in purified water with a propeller mixer (2000 rpm) to prepare a 1% by weight solution. In addition, carbomer was dispersed in purified water with a propeller mixer (4000 rpm) to prepare a 2% by weight containing solution. On the other hand, raw materials 1 to 12 were added to the aqueous phase dissolution tank, stirred and dispersed with a homomixer (2000 rpm), heated to 75° C., and the heated aqueous phase was cooled to room temperature again. After completely dissolving the raw materials 13 to 15 in a separate dissolution tank, the mixture was stirred and mixed into the aqueous phase dissolution tank. Thus, an essence containing the herbal composition of the Examples as an active ingredient was prepared. On the other hand, for the preparation, in addition to the ingredients disclosed in the table, it may further include known ingredients required for manufacturing.

Essence production
ingredient
Content (% by weight)
One
Herbal composition of Examples
0.01
2
glycerin
5.0
3
1,3-butylene glycol
2.0
4
Polyethylene glycol
2.0
5
Carbomer
1.0
6
Sodium hyaluronate
0.1
7
Glycine
3.0
8
Polyacrylamide
2.0
9
Hydroxyethylcellulose
0.2
10
ethanol
3.0
11
Antioxidant
0.3
12
Triethanolamine
0.1
13
Polyoxyethylene cured castor oil
1.0
14
EDTA
0.1
15
antiseptic
Proper
16
Purified water
Balance

Formulation Example 4. Preparation of pack

The composition of the pack containing the herbal composition of the embodiment as an active ingredient is shown in Table 15 below. Specifically, the raw materials 1 to 8 were completely dissolved in a separate dissolution tank, stirred and mixed, and the herbal composition of the Examples was added thereto, followed by sufficiently stirring and mixing to prepare a pack. On the other hand, for the preparation, in addition to the ingredients disclosed in the table, it may further include known ingredients required for manufacturing.

ingredient
Content (% by weight)
One
Herbal composition of Examples
0.01
2
glycerin
7.0
3
1,3-butylene glycol
3.0
4
Squalene
3.0
5
Dimethicone
3.0
6
Sodium hyaluronate
0.1
7
Glycine
2.0
8
Polyacrylamide
5.0
9
Antioxidant
0.3
10
Triethanolamine
0.1
11
EDTA
0.1
12
antiseptic
Proper
13
Purified water
Balance

Formulation Example 5. Preparation of cleansing foam

The composition of the cleansing foam containing the herbal composition of the embodiment as an active ingredient is shown in Table 16 below. After dissolving and dissolving the aqueous phase and the oily phase, respectively, and mixing the mixture, the mixture was cooled to room temperature to prepare a cleansing foam. On the other hand, for the preparation, in addition to the ingredients disclosed in the table, it may further include known ingredients required for manufacturing.

Preparation of cleansing foam
ingredient
Content (% by weight)
One
Herbal composition of Examples
0.01
2
Stearic acid
6.5
3
Myristic acid
28.0
4
Self emulsifying monostearic acid glycerin
3.0
5
Propylene glycol
5.0
6
Concentrated glycerin
10.0
7
Sodium hydroxide
7.0
8
Sodium ethylenediaminetetraacetate
0.1
9
Spices
Proper
10
Purified water
Balance

Formulation Example 6: Preparation of Shampoo

The composition of the shampoo containing the herbal composition of the embodiment as an active ingredient is shown in Table 17 below. The shampoo for hair was prepared according to the conventional method with the ingredients and contents of Table 17 below.

Shampoo manufacturers
ingredient
Content (% by weight)
One
Herbal composition of Examples
5.0
2
Sodium laureth sulfate
1.0
3
Sodium lauryl sulfate
1.0
4
Lauryl glucoside
1.0
5
Decylglucoside
1.0
6
Polyvinyl alcohol
0.05
7
Cetyl alcohol
1.0
8
Glycerin lauryl
0.05
9
incense
0.5
10
Sodium benzoate
0.1
11
Purified water
Balance

Formulation Example 7: Preparation of Shampoo

The composition of the shampoo containing the herbal composition of the embodiment as an active ingredient is shown in Table 18 below. The shampoo for hair was prepared according to the conventional method with the ingredients and contents of Table 18 below.

Shampoo manufacturers
ingredient
Content (% by weight)
One
Herbal composition of Examples
5.0
2
Lauryl glucoside
1.0
3
Decylglucoside
1.0
4
Polyvinyl alcohol
0.05
5
Cetyl alcohol
1.0
6
Glycerin lauryl
0.05
7
incense
0.5
8
Sodium benzoate
0.1
9
Purified water
Balance

As described above, the description of the present invention is for illustration only, and those having ordinary skill in the art to which the present invention pertains can easily be modified into other specific forms without changing the technical spirit or essential features of the present invention. You will understand. Therefore, it should be understood that the above-described embodiments are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the above detailed description, and it should be interpreted that all changes or modified forms derived from the meaning and scope of the claims and equivalent concepts thereof are included in the scope of the present invention. do.

Claims (7)

In the herbal composition for anti-oxidation, wrinkle improvement or inflammation improvement, including extracts of ginseng, saffron, youngsil, ginseng, licorice, hwang, Angelica, hwangnyeon, and eoseongcho,
The herbal composition is 1 to 5: 0.5 ~ 2.5: 0.1 ~ 1: 0.1 ~ 1: 0.5 ~ 2: 1: 0.1 ~ 1: 0.1 ~ The five ginseng, pear tree, Yeongsil, ginseng, licorice, Chihwang, Angelica, Hwangnyeon and Eoseongcho 1: 0.1 ~ 1: 1: 0.5 ~ 2, characterized in that it comprises a weight ratio of antioxidant, anti-wrinkle or inflammation improvement herbal composition.
delete According to claim 1,
The herbal composition
Antioxidant, anti-wrinkle, characterized in that it comprises a weight ratio of 4:1:0.5:0.5:1.5:0.5:0.5:0.5:1 of the ginseng, saffron, youngsil, ginseng, licorice, geohwang, Angelica, hwangnyeon, and eoseongcho Or herbal composition for improving inflammation.
According to claim 1,
The extract
Antioxidant, wrinkle improvement or inflammation characterized by being extracted with one or more extraction solvents selected from the group consisting of water, methanol, ethanol, propanol, butanol, isopropanol, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. Herbal composition for improvement.
A cosmetic composition for antioxidation, wrinkle improvement or inflammation improvement, comprising the herbal composition of claim 1 as an active ingredient.
The method of claim 5,
The cosmetic composition
The cosmetic composition for anti-oxidation, wrinkle improvement or inflammation improvement, comprising the herbal composition in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.
The method of claim 5,
The cosmetic composition
Softening lotion, nourishing lotion, converging lotion, skin, lotion, essence, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing cream, cleansing water, shampoo, conditioner, conditioner, hair gel, Cosmetic composition for anti-oxidation, wrinkle improvement or inflammation improvement, characterized in that it is at least one formulation selected from the group consisting of hair serum, hair essence, body lotion, body wash and body spray.

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