KR102097421B1 - An optical waveguide based sensor and an analyte detection system using it as a key constituent - Google Patents

Abstract

An optical waveguide sensor and a measurement material detection system using the optical waveguide sensor are provided. An optical waveguide sensor according to an embodiment of the present invention includes an optical waveguide that acquires a first light from one side connected to a light source and transmits the second light to the other side; An outer shell formed outside the optical waveguide; And a measuring unit formed by removing a portion of the outer cover so that a portion of the optical waveguide is exposed to the outside, wherein the measuring unit is in contact with a measurement material to be measured using a single DNA strand, and the single The DNA strand is made of any one of SEQ ID NOs: 1 to 5, the measurement substance is mercury, and the single DNA strand is modified into a hairpin when combined with the mercury.

Description

An optical waveguide based sensor and an analyte detection system using it as a key constituent}

The present invention relates to an optical waveguide sensor and a measurement material detection system using the same, and more particularly, to a sensor and system capable of detecting whether heavy metals are included in the measurement material using a surface plasmon resonance phenomenon.

In general, a sensor using an optical waveguide represented by an optical fiber uses voltage, current, temperature, concentration, pressure using various variables such as intensity of light passing through the optical waveguide, refractive index and length of the optical waveguide, and mode and polarization state changes. Various information can be measured. The optical waveguide sensor has the advantage of being capable of ultra-precision wideband measurement, not affected by electromagnetic waves, and easy remote measurement. In addition, since the measurement unit does not use electricity and has excellent corrosion resistance of silica material, there is an advantage that there are almost no restrictions on the use environment.

On the other hand, a chemical sensor or a biosensor for general concentration measurement includes an electrode to use electrical properties of a measurement material. In this case, electricity must be received from the electrode, and there is a problem in the use environment because a conductor such as an electric wire is required to transmit the electric signal measured by the measuring unit to an external measuring device.

In addition, there is an increasing demand for a technology for quickly detecting heavy metals using simple inspection equipment in the field. Currently, various equipments capable of detecting heavy metals have been developed, but there is a need for equipment that satisfies all of mobility, sensitivity, and low cost.

Recently, a technique has been developed to detect heavy metals with super sensitivity using a surface-enhanced Raman spectroscopy technique, but the Raman signal has a problem of very low reproducibility.

KR 10-1226264 B1

In order to solve the problems of the prior art as described above, an embodiment of the present invention provides an optical waveguide sensor and a measurement material detection system using an optical waveguide sensor capable of detecting heavy metals while satisfying both mobility, sensitivity and low cost I want to.

In addition, an embodiment of the present invention is to provide an optical waveguide sensor having excellent reproducibility and a measurement material detection system using the optical waveguide sensor.

According to an aspect of the present invention for solving the above problems, an optical waveguide sensor is provided. The optical waveguide sensor includes an optical waveguide that acquires a first light from one side connected to a light source and transmits the second light to the other side; An outer shell formed outside the optical waveguide; And a measuring unit formed by removing a portion of the outer cover so that a portion of the optical waveguide is exposed to the outside, wherein the measuring unit is in contact with a measurement material to be measured using a single DNA strand, and the single The DNA strand is made of any one of SEQ ID NOs: 1 to 5, the measurement substance is mercury, and the single DNA strand is modified into a hairpin when combined with the mercury.

The measurement unit may further include a plating layer formed on the surface of the optical waveguide exposed to the outside to bind at least one single DNA strand.

The plating layer may include a chromium plating layer that is a first layer formed on the surface of the optical waveguide; And a gold plating layer, which is a second layer formed on the surface of the chromium plating.

The single DNA strand may be any one of SEQ ID NOs: 1 to 5.

The optical waveguide may be provided so that the other side is connected to an optical output meter that measures the output of the second light.

The light source may be a helium-neon laser or a tungsten-halogen lamp.

The measurement material is a heavy metal, and the detection limit of the measurement unit may be expressed by the following equation.

(Where LOD is the detection limit, SD = standard deviation of output at the first concentration, ΔP = output of the first concentration, ΔC = concentration difference between the first concentration and the second concentration)

The measurement material may be mercury.

According to an aspect of the present invention, a measurement material detection system using an optical waveguide sensor is provided. The measurement material detection system using the optical waveguide sensor includes: a light source that generates a first light of a certain intensity; An optical waveguide sensor that receives the first light from the light source and converts the first light into a second light using a measurement material; And a detection device that receives the second light from the optical waveguide sensor and detects whether a heavy metal to be measured is contained in the measurement material using a single DNA strand.

The light source may be a helium-neon laser or a tungsten-halogen lamp.

An optical waveguide that acquires the first light from one side connected to the light source and transmits the second light to the other side; An outer shell formed outside the optical waveguide; And a measuring unit formed by removing a portion of the outer cover so that a portion of the optical waveguide is exposed to the outside, wherein the measuring unit can contact a measurement material to measure concentration using the single DNA strand. .

The measurement unit may further include a plating layer formed on the surface of the optical waveguide exposed to the outside to bind at least one single DNA strand.

The plating layer may include a chromium plating layer that is a first layer formed on the surface of the optical waveguide; And a gold plating layer, which is a second layer formed on the surface of the chromium plating.

The single DNA strand may be any one of SEQ ID NOs: 1 to 5.

The optical waveguide may be provided so that the other side is connected to an optical output meter that measures the output of the second light.

The light source may be a helium-neon laser or a tungsten-halogen lamp.

The measurement material is a heavy metal, and the detection limit of the measurement unit may be expressed by the following equation.

(Where LOD is the detection limit, SD = standard deviation of output at the first concentration, ΔP = output of the first concentration, ΔC = concentration difference between the first concentration and the second concentration)

The measurement material may be mercury.

The optical waveguide sensor and the measurement material detection system using the same according to an embodiment of the present invention have an effect of having high reproducibility when repeatedly detecting a measurement material.

In addition, the optical waveguide sensor and the measurement material detection system using the same according to an embodiment of the present invention have an effect capable of detecting the presence of heavy metals at a low cost.

In addition, the optical waveguide sensor and the measurement material detection system using the same according to an embodiment of the present invention have an effect capable of measuring the concentration of heavy metals in real time.

In addition, the optical waveguide sensor and the measurement material detection system using the same have the effect of detecting heavy metals with high sensitivity.

1 is a view showing an optical waveguide sensor according to an embodiment of the present invention.
2 is a view showing an optical waveguide sensor coupled with a single DNA strand according to an embodiment of the present invention.
3 is a view showing a change in the morphology of a single DNA strand when detecting (a) the basic form of a single DNA strand of SEQ ID NO: 1 and (b) mercury ions according to an embodiment of the present invention.
FIG. 4 is a diagram showing a change in the morphology of a single DNA strand when (a) detecting the basic form of a single DNA strand of SEQ ID NO: 2 and (b) mercury ions according to an embodiment of the present invention.
5 is a view showing a change in form of a single DNA strand when detecting the basic form of (a) a single DNA strand of SEQ ID NO: 3 and (b) mercury ions according to an embodiment of the present invention.
6 is a view showing a change in the morphology of a single DNA strand when (a) detecting the basic form of a single DNA strand of SEQ ID NO: 4 and (b) mercury ions according to an embodiment of the present invention.
7 is a view showing a change in the morphology of a single DNA strand when detecting the basic form of (a) a single DNA strand of SEQ ID NO: 5 and (b) mercury ions according to an embodiment of the present invention.
8 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 1 according to an embodiment of the present invention.
9 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 2 according to an embodiment of the present invention.
10 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 3 according to an embodiment of the present invention.
11 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 4 according to an embodiment of the present invention.
12 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 5 according to an embodiment of the present invention.
13 is a diagram showing a summary of the results of the detection limits of SEQ ID NO: 1 to SEQ ID NO: 5 according to an embodiment of the present invention.
14 is a view showing a measurement material detection system using an optical waveguide sensor according to an embodiment of the present invention.
15 is a view showing a measurement material detection system using an optical waveguide sensor according to an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains may easily practice. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. In the drawings, parts not related to the description are omitted in order to clearly describe the present invention, and the same reference numerals are attached to the same or similar elements throughout the specification.

1 is a view showing an optical waveguide sensor according to an embodiment of the present invention, Figure 2 is a view showing an optical waveguide sensor combined with a single DNA strand according to an embodiment of the present invention, Figure 3 is an embodiment of the present invention According to (a) a basic form of a single DNA strand of SEQ ID NO: 1 and (b) a diagram showing a change in the form of a single DNA strand when detecting mercury ions, FIG. 4 is a sequence of (a) according to an embodiment of the present invention Figure 2 shows the basic morphology of a single DNA strand and (b) a change in morphology of a single DNA strand when mercury ions are detected, and FIG. 5 shows a single DNA strand of (a) SEQ ID NO: 3 according to an embodiment of the present invention. The basic form of (b) is a diagram showing the morphological change of a single DNA strand when sensing mercury ions, Figure 6 is (a) the basic form of a single DNA strand of SEQ ID NO: 4 according to an embodiment of the present invention and (b ) Changes in the shape of a single DNA strand when mercury ions are detected FIG. 7 is a diagram showing a (a) basic form of a single DNA strand of SEQ ID NO: 5 according to an embodiment of the present invention and (b) a change in the form of a single DNA strand when detecting mercury ions, FIG. 8 Is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 1 according to an embodiment of the present invention, and FIG. 9 shows the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 2 according to an embodiment of the present invention 10 is a diagram showing the results of a detection limit experiment for a single DNA strand of SEQ ID NO: 3 according to an embodiment of the present invention, and FIG. 11 is a single DNA strand of SEQ ID NO: 4 according to an embodiment of the present invention. It is a diagram showing the results of the detection limit for the experiment, Figure 12 is a diagram showing the results of the detection limit for the single DNA strand of SEQ ID NO: 5 according to an embodiment of the present invention, Figure 13 is a sequence number according to an embodiment of the present invention 1 to It is a diagram showing the results of the detection limit experiment of SEQ ID NO: 5 in a summary table.

Hereinafter, an optical waveguide sensor according to an embodiment of the present invention will be described in more detail with reference to FIGS. 1 to 8.

Referring to FIG. 1, an optical waveguide sensor 100 according to an embodiment of the present invention includes an optical waveguide 110, a measuring unit 120, and an outer shell 130. At this time, the optical waveguide sensor 100 may be formed by having an outer shell 130 outside the optical waveguide 110. In addition, the optical waveguide 110 may be formed of, for example, silica-based, the outer shell 130 may be formed of a polymer type, the refractive index of the optical waveguide 110 may be formed larger than the refractive index of the outer shell 130 have.

The measuring unit 120 contacts the sample and detects a measurement substance contained in the sample. To this end, the measurement unit 120 is formed to include the first plating layer 121 and the second plating layer 123 in the exposed optical waveguide 110 by partially removing the outer shell 130 of the optical waveguide sensor 100.

In other words, the measurement unit 120 is a portion formed by sequentially stacking the first metal layer 121 and the second metal layer 123 after the optical waveguide 110 is exposed to the outside, particularly as illustrated in FIG. 2. Likewise, one side of the single DNA strand 210 may be formed to bind to the second metal layer 123. In addition, the first plating layer 121 may be preferably a chrome plating layer, and the second plating layer 123 may be a gold plating layer.

At least one single DNA strand 210 is provided, and one side is coupled to the second metal layer 123. In this case, one side of the single DNA strand 210 binding to the second metal layer 123 may be a 5 'side to which a siol group (SH-) is coupled, and a 3' side may be formed to contact the sample 230. In addition, the single DNA strand 210 according to an embodiment of the present invention is most preferably using any one of the five single-stranded DNA sequences described below, but is not necessarily limited thereto.

1 and 2 show a cross-sectional view of the optical waveguide sensor 100 for convenience of explanation and understanding. In the present invention, the optical waveguide 110 exposed to the outside by removing the outer shell 130 may appear in a cylindrical shape. . In addition, the optical waveguide sensor 100 of the present invention, for example, the diameter of the optical waveguide 110 may be formed in about 200um, the thickness of the outer shell may be formed in about 15um.

Further, the measurement unit 120 may have a length of 4 cm, but the present invention is not limited thereto and may be formed in various lengths according to a user's setting. In addition, the measurement material included in the sample 230 may be a heavy metal, particularly preferably mercury.

Meanwhile, FIGS. 3 to 7 show basic forms of five single DNA strands used in the optical waveguide sensor 100 according to an embodiment of the present invention, and forms when mercury ions are detected, respectively.

Table 1 below shows the base sequences of five single DNA strands shown in FIGS. 3 to 7.

Sequence Sequence number 5'- TTG TTT GTT GCC CCC TTC TTT CTT -3 ' One 5'- TTC TTT CTT CCC CCC TTC TTT CTT -3 ' 2 5'- TCG TTC GTC GCC CCC CTC CTT CCT -3 ' 3 5'- TTG TCC GCC GCC CCC CCC CCT CTT -3 ' 4 5'- CCG CCT GTT GCC CCC TTC TCC CCC -3 ' 5

Looking at Figure 3a, the single DNA of SEQ ID NO: 1 is formed in a form in which the 5 'side contacts the second plating layer 123, and the internal base sequence is formed in the order of TTG TTT GTT GCC CCC TTC TTT CTT.

At this time, when the measurement unit 120 comes into contact with the sample 230, the single DNA of SEQ ID NO: 1 in FIG. 3A is transformed into the hairpin shape of FIG. 3B so that mercury ions bind to two thymines. At this time, mercury ions are bound between thymine and thymine, and guanine and cytosine are formed in a binding form. That is, the single DNA of SEQ ID NO: 1 shown in FIG. 3 is formed to have a total of 7 thymine-thymine bonds and 3 guanine-cytosine bonds.

Looking at Figure 4a, the single DNA of SEQ ID NO: 2 is formed in a form in which the 5 'side contacts the second plating layer 123, and the internal base sequence is formed in the order of TTC TTT CTT CCC CCC TTC TTT CTT.

At this time, when the measurement unit 120 comes into contact with the sample 230, the single DNA of SEQ ID NO: 2 in FIG. 4A is transformed into the hairpin shape of FIG. 4B so that mercury ions bind to two thymines. At this time, mercury ions are bound between thymine and thymine, and guanine and cytosine are formed in a binding form. That is, the single DNA of SEQ ID NO: 2 shown in FIG. 4 has a total of seven thymine-thymine bonds, and unlike the single DNA of SEQ ID NO: 1, guanine-cytosine bond is not formed.

Looking at Figure 5a, the single DNA of SEQ ID NO: 3 is formed in a form in which the 5 'side contacts the second plating layer 123, and the internal base sequence is formed in the order of TCG TTC GTC GCC CCC CTC CTT CCT.

At this time, when the measurement unit 120 comes into contact with the sample 230, the single DNA of SEQ ID NO: 3 in FIG. 5A is transformed into the hairpin shape of FIG. 5B so that mercury ions bind to two thymines. At this time, mercury ions are bound between thymine and thymine, and guanine and cytosine are formed in a binding form. That is, the single DNA of SEQ ID NO: 3 shown in FIG. 5 has a total of 4 thymine-thymine bonds and 3 guanine-cytosine bonds, and is formed to have 3 thymine-thymine bonds less than the single DNA of SEQ ID NO: 1. .

Referring to Figure 6a, the single DNA of SEQ ID NO: 4 is formed in a form in which the 5 'side is in contact with the second plating layer 123, and the internal base sequence is formed in the order of TTG TCC GCC GCC CCC CCC CCT CTT.

At this time, when the measurement unit 120 comes into contact with the sample 230, the single DNA of SEQ ID NO: 4 in FIG. 6A is transformed into the hairpin shape of FIG. 6B so that mercury ions bind to two thymines. At this time, mercury ions are bound between thymine and thymine, and guanine and cytosine are formed in a binding form. That is, the single DNA of SEQ ID NO: 4 shown in FIG. 6 has a total of three thymine-thymine bonds and three guanine-cytosine bonds, and is formed to have four thymine-thymine bonds less than the single DNA of SEQ ID NO: 1. .

Looking at Figure 7a, the single DNA of SEQ ID NO: 5 is formed in a form in which the 5 'side contacts the second plating layer 123, and the internal base sequence is formed in the order of CCG CCT GTT GCC CCC TTC TCC CCC.

At this time, when the measurement unit 120 comes into contact with the sample 230, the single DNA of SEQ ID NO: 5 in FIG. 7A is transformed into the hairpin shape of FIG. 7B so that mercury ions bind to two thymines. At this time, mercury ions are bound between thymine and thymine, and guanine and cytosine are formed in a binding form. That is, the single DNA of SEQ ID NO: 5 shown in FIG. 7 has a total of three thymine-thymine bonds and three guanine-cytosine bonds, and is formed to have four thymine-thymine bonds less than the single DNA of SEQ ID NO: 1. .

Here, the structure of a single DNA of SEQ ID NO: 4 and a single DNA of SEQ ID NO: 5 may be classified according to whether a thymine-thymine bond exists in the tail portion or a thymine-thymine bond exists in the hairpin portion.

On the other hand, Figure 8 is the detection limit test results for a single DNA strand of SEQ ID NO: 1 according to an embodiment of the present invention, Figure 9 is the detection limit experiment results for a single DNA strand of SEQ ID NO: 2 according to an embodiment of the present invention, 10 is a detection limit test result for a single DNA strand of SEQ ID NO: 3 according to an embodiment of the present invention, Figure 11 is a detection limit experiment result for a single DNA strand of SEQ ID NO: 4 according to an embodiment of the present invention, Figure 12 Is a detection limit test result for a single DNA strand of SEQ ID NO: 5 according to an embodiment of the present invention and FIG. 13 is a diagram showing a detection limit test result of SEQ ID NO: 1 to SEQ ID NO: 5 according to an embodiment of the present invention in a summary table . At this time, the detection limit is generally called a limit of detection (LOD), and can be calculated through the following equation using a standard deviation.

Equation 1

Here, LOD is the detection limit, SD is the standard deviation of the output at the first concentration, ΔP is the output value of the first concentration, and ΔC can be the concentration difference between the first concentration and the second concentration.

8 shows the results of a detection limit experiment using a single DNA strand of SEQ ID NO: 1. The output-mercury ion concentration graph of FIG. 8 is a graph showing measured output values according to changes in mercury ion concentration, and the values are summarized in the table on the right.

At this time, the detection limit of the single DNA strand of SEQ ID NO: 1 can be calculated by the following equation (2).

Equation 2

M의 검출 한계를 가지는 것을 확인하였다.That is, as a result of calculation, a single DNA of SEQ ID NO: 1 is about

It was confirmed to have a detection limit of M.

Next, Figure 9 shows the results of the detection limit experiment using a single DNA strand of SEQ ID NO: 2. The output-mercury ion concentration graph of FIG. 9 is a graph showing measured output values according to changes in mercury ion concentration, and the values are summarized in the table on the right.

At this time, the detection limit of the single DNA strand of SEQ ID NO: 2 can be calculated by the following equation (3).

Equation 3

M의 검출 한계를 가지는 것을 확인하였다.That is, as a result of calculation, the single DNA of SEQ ID NO: 2 is about

It was confirmed to have a detection limit of M.

Next, FIG. 10 shows the results of a detection limit experiment using a single DNA strand of SEQ ID NO: 3. The output-mercury ion concentration graph of FIG. 10 is a graph showing measured output values according to changes in mercury ion concentration, and the values are summarized in the table on the right.

At this time, the detection limit of the single DNA strand of SEQ ID NO: 3 may be calculated by Equation 4 below.

Equation 4

M의 검출 한계를 가지는 것을 확인하였다.That is, as a result of calculation, the single DNA of SEQ ID NO: 3 is about

It was confirmed to have a detection limit of M.

Next, Figure 11 shows the results of the detection limit experiment using a single DNA strand of SEQ ID NO: 4. The output-mercury ion concentration graph of FIG. 11 is a graph showing measured output values according to changes in mercury ion concentration, and the values are summarized in the table on the right.

At this time, the detection limit of the single DNA strand of SEQ ID NO: 4 can be calculated by the following equation (5).

Equation 5

M의 검출 한계를 가지는 것을 확인하였다.That is, as a result of calculation, the single DNA of SEQ ID NO: 4 is about

It was confirmed to have a detection limit of M.

Finally, Figure 12 shows the results of the detection limit experiment using a single DNA strand of SEQ ID NO: 5. The output-mercury ion concentration graph of FIG. 12 is a graph showing measured output values according to changes in mercury ion concentration, and the values are summarized in the table on the right.

At this time, the detection limit of the single DNA strand of SEQ ID NO: 5 may be calculated by Equation 6 below.

Equation 6

M의 검출 한계를 가지는 것을 확인하였다.That is, as a result of calculation, the single DNA of SEQ ID NO: 5 is about

It was confirmed to have a detection limit of M.

Meanwhile, the results of FIGS. 8 to 12 described above are tabulated in FIG. 13. In this case, when comparing SEQ ID NO: 1 and SEQ ID NO: 2, the same number of thymine-thymine bonds exist, but since no guanine-cytosine bond exists in SEQ ID NO: 2, a single DNA structure of SEQ ID NO: 2 detects mercury ions It was confirmed that the ability was lower than that of the single DNA structure of SEQ ID NO: 1.

Further, when comparing SEQ ID NO: 1 and SEQ ID NO: 3, the two single DNAs have the same number of guanine-cytosine bonds, but SEQ ID NO: 3 forms three fewer thymine-thymine bonds than SEQ ID NO: 1, thus mercury It was confirmed that the ion detection ability was lower than that of the single DNA structure of SEQ ID NO: 1. Furthermore, through comparison of detection limit values with SEQ ID NO: 2, it was confirmed that the number of thymine-thymine bonds has a more direct effect on the detection limit.

In addition, when comparing SEQ ID NO: 4 and SEQ ID NO: 5, as shown in FIGS. 6 and 7, SEQ ID NO: 4 forms thymine-thymine bond on the tail side, and SEQ ID NO: 5 forms thymine-thymine bond on the hairpin side. , As a result, it was confirmed that SEQ ID NO: 4 can detect mercury ions more sensitively than SEQ ID NO: 5.

8 to 12, the optical waveguide sensor 100 of the present invention has a single DNA structure that binds to the measurement unit 120, the more thymine-thymine bonds, the more guanine-cytosine bonds. It was confirmed that mercury ions can be sensitively detected, and when the same number of binding ions are present, a single DNA structure in which thymine-thymine binding is present on the tail side can sensitively detect mercury ions.

Meanwhile, FIG. 14 shows a measurement material detection system using an optical waveguide sensor according to an embodiment of the present invention.

Referring to FIG. 14, a measurement material detection system 1400 using an optical waveguide sensor according to an embodiment of the present invention includes a light source 1410, a light conversion unit 1420, an optical waveguide sensor 100 and an optical measurement unit (1430).

The light source 1410 generates first light therein and transmits it to the light conversion unit 1420. At this time, the output of the first light generated by the light source 210 is adjustable according to a user's setting, and is preferably visible light. This is because the amount of light absorbed by the aqueous solution in the visible light region is hardly present. Therefore, the light source 210 may be a device that generates and emits visible light such as a laser diode or an LED, and may be a helium-neon laser or a tungsten-halogen lamp in any one embodiment of the present invention. have.

Next, the light conversion unit 1420 receives the first light and performs light conversion for transmitting parallel light to the optical waveguide sensor 100. In this case, the light conversion unit 1420 may provide parallel light using a lens having various curvatures, for example, and use a mirror, a quarter wave plate, and an optical separator to convert the parallel light to the optical waveguide sensor 100 ). Furthermore, the present invention is not limited to this, and may be sufficiently omitted according to a user's setting.

The optical waveguide sensor 100 receives the first light from the light conversion unit 1420 and emits the second light, and the light measurement unit 1430 receives the second light and outputs the second light and the first light. Compare and confirm that mercury ions are included in the sample. At this time, when the output of the second light is reduced compared to the output of the first light, the light measurement unit 1430 may determine that the sample contains mercury ions.

Although one embodiment of the present invention has been described above, the spirit of the present invention is not limited to the embodiments presented herein, and those skilled in the art to understand the spirit of the present invention may add elements within the scope of the same spirit. However, other embodiments may be easily proposed by changing, deleting, adding, or the like, but this will also be considered to be within the scope of the present invention.

100: optical waveguide sensor 110: optical waveguide
120: measuring unit 121: the first metal layer
123: second metal layer 130: outer shell
210: single DNA strand 230: sample
1400: Measurement substance detection system using optical waveguide sensor
1410: light source 1420: light conversion unit
1430: light measuring unit

<110> Gachon University of Industry-Academic cooperation Foundation <120> Optical waveguide sensor and measuring compound detecting system          with in <130> 180604-0001 <160> 5 <170> KoPatentIn 3.0 <210> 1 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Thiol-modified ssDNA <400> 1 ttctttcttc cccccttctt tctt 24 <210> 2 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Thiol-modified ssDNA <400> 2 ttctttcttc cccccttctt tctt 24 <210> 3 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Thiol-modified ssDNA <400> 3 tcgttcgtcg cccccctcct tcct 24 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Thiol-modified ssDNA <400> 4 ttgtccgccg cccccccccc tctt 24 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Thiol-modified ssDNA <400> 5 ccgcctgttg cccccttctc cccc 24

Claims (18)

An optical waveguide that acquires the first light from one side connected to the light source and transmits the second light to the other side;
An outer shell formed outside the optical waveguide; And
Includes a measuring unit formed by removing a portion of the outer shell so that a portion of the optical waveguide is exposed to the outside,
The measurement unit is in contact with the measurement material to measure the concentration using a single DNA strand,
The single DNA strand is made of any one of SEQ ID NO: 1 to 5,
The measurement material is mercury,
When the single DNA strand is combined with the mercury, the optical waveguide sensor is transformed into a hairpin. According to claim 1,
The measurement unit, the optical waveguide sensor further comprises; a plating layer formed on the surface of the optical waveguide exposed to the outside to bind to the at least one single DNA strand. According to claim 2,
The plating layer may include a chromium plating layer that is a first layer formed on the surface of the optical waveguide; And
An optical waveguide sensor formed of a gold plating layer, which is a second layer formed on the surface of the chrome plating. delete According to claim 3,
The optical waveguide sensor is provided so that the other side is connected to the optical output measuring device for measuring the output of the second light. The method of claim 5,
The light source is an optical waveguide sensor that is a helium-neon laser or a tungsten-halogen lamp. The method of claim 6,
The measurement material is a heavy metal, and an optical waveguide sensor in which the detection limit of the measurement unit is expressed by the following equation.

(Where LOD is the detection limit, SD = standard deviation of output at the first concentration, ΔP = output of the first concentration, ΔC = concentration difference between the first concentration and the second concentration) delete A light source that generates a first light of a certain intensity;
An optical waveguide sensor that receives the first light from the light source and converts the first light into a second light using a measurement material; And
It includes; a detection device for receiving the second light from the optical waveguide sensor and detecting whether a heavy metal to be measured is included in the measurement material using a single DNA strand;
The single DNA strand is made of any one of SEQ ID NO: 1 to 5,
The measurement material is mercury,
When the single DNA strand is combined with the mercury, a measurement substance detection system using an optical waveguide sensor that is transformed into a hairpin shape. The method of claim 9,
The light source is a helium-neon laser or tungsten-halogen lamp, an optical waveguide sensor, a measurement material detection system. The method of claim 10,
An optical waveguide that acquires the first light from one side connected to the light source and transmits the second light to the other side;
An outer shell formed outside the optical waveguide; And
Includes a measuring unit formed by removing a portion of the outer shell so that a portion of the optical waveguide is exposed to the outside,
The measurement unit, a measurement material detection system using an optical waveguide sensor in contact with the measurement material to measure the concentration using the single DNA strand. The method of claim 11,
The measurement unit, a measurement material detection system using an optical waveguide sensor further comprising; a plating layer formed on the surface of the optical waveguide exposed to the outside to bind to the at least one single DNA strand. The method of claim 12,
The plating layer may include a chromium plating layer that is a first layer formed on the surface of the optical waveguide; And a gold plating layer, which is a second layer formed on the surface of the chromium plating; a measurement material detection system using an optical waveguide sensor. delete The method of claim 13,
The optical waveguide, a measurement material detection system using an optical waveguide sensor is provided so that the other side is connected to an optical output meter for measuring the output of the second light. The method of claim 15,
The light source is a helium-neon laser or a tungsten-halogen lamp, an optical waveguide sensor for measuring material detection system. The method of claim 16,
The measurement material is a heavy metal, and a measurement material detection system using an optical waveguide sensor in which the detection limit of the measurement unit is expressed by the following equation.

(Where LOD is the detection limit, SD = standard deviation of output at the first concentration, ΔP = output of the first concentration, ΔC = concentration difference between the first concentration and the second concentration) delete

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