KR101629848B1

KR101629848B1 - Method for reproducing sensor head of biosensor

Info

Publication number: KR101629848B1
Application number: KR1020140160598A
Authority: KR
Inventors: 주형규; 안성수
Original assignee: 가천대학교 산학협력단
Priority date: 2014-11-18
Filing date: 2014-11-18
Publication date: 2016-06-14
Also published as: KR20160059500A

Abstract

The present invention is a method of regenerating a sensor head of a biosensor, which comprises a sensor providing step, a first rinsing step, and a second rinsing step. The sensor providing step includes a sensor head composed of an optical fiber core, a metal layer laminated on the optical fiber core, and a nickel layer laminated on the metal layer, a histidine tag peptide bonded to the nickel layer at one end of histidine and a peptide tag at the other end of the histidine tag peptide And an antibody that binds to the target antigen and captures the target antigen. The first rinsing step injects a separate solution in the nickel layer to break the binding of the histidine and nickel layers to separate the histidine tag peptide and antibody from the nickel layer. The second rinsing step injects the acidic solution into the nickel layer to clean the separating solution. After the first rinsing step and the second rinsing step, the histidine tag peptide and the antibody are removed from the sensor head so that the sensor head can be regenerated.

Description

TECHNICAL FIELD [0001] The present invention relates to a method of regenerating a sensor head of a biosensor,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method of regenerating a sensor head of a biosensor in which a sensor head of a biosensor is regenerated so that a sensor head can be reused after using a non- .

A biosensor is a biosensor that recognizes a specific substance by combining it with an electric or optical transducer to convert biological interaction and recognition reaction into electrical or optical signals to selectively detect trace amounts of biochemicals .

Conventionally, a biosensor adopts a method of attaching a fluorescent label to a target biomaterial and then detecting a biomaterial using an optical scanner or the like, and a large-scale analysis There is a problem that a device is required and a variety of target materials must be uniformly labeled with a fluorescent material. In addition, there was a fear that structural changes of biomaterials due to labeling could occur, real-time monitoring was impossible, various preparatory steps for labeling were cumbersome, and there was a problem of contamination.

Non-labeled biosensor technologies are being studied to solve these problems. A non-labeled biosensor using optical components is composed of a sensor head to which a light signal is transmitted and an antibody to be coupled to the sensor head. After capturing a target antigen to be detected in the antibody, a light signal is transmitted through the sensor head, Lt; RTI ID = 0.0 > quantitatively < / RTI >

However, in the case of a non-labeled biosensor using a conventional optical component, there is a problem that the biosensor must be discarded because reproduction after use is impossible.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a sensor head in which an optical signal is transmitted and a biosensor composed of an antibody coupled to the sensor head, And a method of regenerating a sensor head of a biosensor which can prevent waste of resources and costs by removing antibodies.

In order to accomplish the above object, the present invention provides a method of regenerating a sensor head of a biosensor, the method comprising: an optical fiber core; a metal layer laminated on the optical fiber core; a sensor head composed of a nickel layer laminated on the metal layer; Providing a biosensor having a histidine tag peptide bound to the nickel layer and an antibody binding to a peptide at the other end of the histidine tag peptide and capturing a target antigen; In order to prevent the signal distortion of the biosensor and improve the durability of the nickel layer, a barrier layer for blocking the binding of the target antigen to the nickel layer is applied to the nickel layer to form a barrier layer step; A first rinsing step of injecting into the nickel layer a separation solution which breaks bonds of the histidine and the nickel layer to separate the histidine tag peptide and the antibody from the nickel layer; And a second rinsing step of injecting an acidic solution into the nickel layer to clean the separation solution, wherein the histidine tag peptide and the antibody from the sensor head after the first rinsing step and the second rinsing step So that the sensor head can be regenerated.

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In the method of regenerating the sensor head of the biosensor according to the present invention, the separation solution in the first rinsing step may include an immidazole solution.

In the method of regenerating the sensor head of the biosensor according to the present invention, the acid solution in the second rinsing step may include an acetic acid solution.

In the method of regenerating the sensor head of the biosensor according to the present invention, the metal layer may include a double layer structure including a gold (Au) layer laminated on the optical fiber core and an aluminum (Al) layer laminated on the gold layer, Layer structure including a silver (Ag) layer laminated on the core and an aluminum (Al) layer laminated on the silver layer.

According to the method for regenerating the sensor head of the biosensor of the present invention, waste of resources and cost can be prevented.

In addition, according to the method for regenerating the sensor head of the biosensor of the present invention, signal distortion of the biosensor can be prevented and durability of the nickel layer repeatedly used in the regenerated sense head can be improved.

1 is a view showing a biosensor provided in a method for regenerating a sensor head of a biosensor according to an embodiment of the present invention,
FIG. 2 is a view for explaining a principle of detecting a target antigen using the biosensor of FIG. 1,
FIG. 3 is a diagram sequentially illustrating a method of regenerating a sensor head of a biosensor according to an embodiment of the present invention,
FIG. 4 is a diagram for illustrating the performance of the sensor head reproduced by the method of reproducing the sensor head of the biosensor of FIG. 3;

Hereinafter, embodiments of a method of regenerating a sensor head of a biosensor according to the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a view showing a biosensor provided in a method of regenerating a sensor head of a biosensor according to an embodiment of the present invention. FIG. 2 illustrates a principle of detecting a target antigen using the biosensor of FIG. FIG. 3 is a view sequentially showing a method of reproducing a sensor head of a biosensor according to an embodiment of the present invention, FIG. 4 is a view illustrating a method of reproducing a sensor head reproduced by the method of reproducing a sensor head of the biosensor of FIG. Fig. 6 is a view for showing the performance of the head. Fig.

Referring to FIGS. 1 to 4, the method for regenerating the sensor head of the biosensor of the present embodiment is for reproducing the sensor head of the biosensor so that the sensor head can be reused after using the biosensor of the non- (S10), a barrier layer forming step (S20), a first rinsing step (S30), and a second rinsing step (S40).

The sensor providing step S10 provides the biosensor 20 in which the sensor head 10 is to be reproduced. In this embodiment, the biosensor 20 includes a sensor head 10, a histidine tag peptide 21, and an antibody 22.

1, the sensor head 10 is composed of an optical fiber core 11, a metal layer 12 stacked on the optical fiber core 11, and a nickel layer 13 stacked on the metal layer 12. The cladding is stripped from the optical fiber including the silica core 11 and the cladding made of the polymer material and the metal layer 12 is deposited on the optical fiber core 11 from which the cladding is removed. At this time, the metal layer 12 is formed of a bilayer structure (bi-metal) including gold (Au) 12a laminated on the optical fiber core 11 and aluminum (Al) 12b laminated on the gold 11 Layer structure including silver (Ag) laminated on the optical fiber core 11 and aluminum (Al) 12b laminated on the silver. After the metal layer 12 is formed, a nickel layer 13 is laminated on the metal layer 12.

Metal vapor deposition (Metal Vapor Deposition) is useful as a method of laminating the metal layer 12 and the nickel layer 13, among which thermal evaporation can be used. The metal layer 12 and the nickel layer 13 may be formed by various methods such as sputtering and E-beam evaporation.

A histidine-tagged peptide 21 has a structure in which histidine is connected to one end 21a and peptide is connected to the other end 21b. One end (21a) of the histidine tag peptide to which histidine is linked is bound to the nickel layer (13).

Antibody 22 binds to the peptide at the other end 21b of histidine tag peptide 21 and captures target antigen 1. For example, after the antibody 22, such as IgG, is bound to the histidine tag peptide 21, a target antigen 1 such as a fibrinogen can be captured. Fibrinogen is a protein extracted from blood plasma and is known as a protein that acts as a marker for dementia.

2, circularly polarized incident light S1 incident on a waveguide, such as an optical fiber core 11, is guided along the optical fiber core 11 (S2) and is sensed with the metal layer 12 through the antibody 22 The polarization of the emitted light S3 is changed by experiencing excitation according to the surface plasmon resonance (SPR) via the target antigen 1 and analyzed to find the concentration of the target antigen 1 . The progress of dementia can be determined through the concentration information of the target antigen (1) such as fibrinogen.

The blocking layer forming step S20 forms a blocking layer 50 by applying a blocking solution for blocking the binding of the target antigen 1 to the nickel layer 13 to the nickel layer 13. At this time, the barrier layer formation step (S20) is preferably performed before the first rinsing step (S30).

In the absence of the blocking layer 50, a portion of the target antigen 1 may be bound to the antibody 22, while the other portion may be bound to the nickel layer 13. The non-specific binding between the target antigen 1 and the nickel layer 13 causes distortion of the optical signal propagating along the optical fiber core 11 by the target antigen 1 bonded to the nickel layer 13, The information of the antigen 1 can not be accurately grasped.

If the non-specific binding between the target antigen 1 and the nickel layer 13 is performed, the nickel layer 13 may be damaged in the first and second rinsing steps S30 and S40. The biosensor 20 can be manufactured by recycling the sensor head 10 even after regenerating the sensor head 10 of the biosensor as long as the nickel layer 13 is maintained in a good state.

Therefore, before using the biosensor 20, the barrier layer 50 may be formed on the nickel layer 13 to prevent signal distortion of the biosensor 20 and enhance the durability of the nickel layer 13 .

In the first rinsing step S30, a separation solution 30 for dissociating the binding of histidine and the nickel layer 13 is injected into the nickel layer 13. The histidine tag peptide 21 and the antibody 22 must first be separated from the nickel layer 13 in order to regenerate the sensor head 10 of the biosensor. Thus, the blocking layer 50, the histidine tag peptide 21 and the antibody (SEQ ID NO: 2) can be removed by injecting the separation layer 30, which breaks the bond between the histidine of the histidine tag peptide 21 and the nickel layer 13, 22 may be removed from the nickel layer 13.

The separation solution in the first rinsing step (S30) of this embodiment is preferably an immidazole solution.

The second rinsing step (S40) injects the acidic solution (40) into the nickel layer (13). After performing the first rinsing step S30, residues of the histidine tag peptide 21 and the separation solution 30 may be left on the nickel layer 13. By injecting the acidic solution 40 into the nickel layer 13, the residue left in the nickel layer 13 can be cleanly cleaned.

The acid solution 40 of the second rinsing step S40 of the present embodiment is preferably an acetic acid solution.

The histidine tag peptide 21 and the antibody (hereinafter, referred to as " antibody ") are separated from the sensor head 10 through the sensor providing step S10, the barrier layer forming step S20, the first rinsing step S30 and the second rinsing step S40. The sensor head 10 composed of the optical fiber core 11, the metal layer 12 and the nickel layer 13 can be obtained again and the histidine tag 10 The peptide 21 and the antibody 22 can be combined to be reused as the biosensor 20.

4 is a graph for confirming the performance of the sensor head 10 reproduced by the method of reproducing the sensor head of the biosensor of the present embodiment.

4, when the histidine tag peptide 21 is bound to the sensor head 10, the output value of the optical signal output via the optical fiber core 11 and the output value of the separation solution 30 and the acid solution 40 are used The output values of the optical signals output through the optical fiber core 11 after the first rinsing step S30 and the second rinsing step S40 are displayed substantially the same.

It is confirmed that the performance of the sensor head 10 reproduced through the first rinsing step S30 and the second rinsing step S40 is restored to that of the sensor head 10 before the regeneration.

The method of regenerating the sensor head of the biosensor according to the present invention as described above includes the steps of using a biosensor comprising a sensor head to which an optical signal is transmitted and an antibody coupled to the sensor head, By removing the antibody from the head, it is possible to prevent waste of resources and cost.

The method of regenerating the sensor head of the biosensor according to the present invention configured as described above includes the steps of forming a barrier layer for blocking the binding of the target antigen to the nickel layer on the nickel layer to prevent signal distortion of the biosensor And the durability of the nickel layer repeatedly used in the recovered sensing head can be improved.

The scope of the present invention is not limited to the above-described embodiments and modifications, but can be implemented in various forms of embodiments within the scope of the appended claims. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the present invention as defined by the appended claims.

10: Sensor head
11: Optical fiber core
12: metal layer
13: Nickel layer
20: Biosensor
21: histidine tag peptide
22: Antibody
30: Separation solution
40: Acid solution

Claims

A histidine tag peptide in which histidine at one end is bonded to the nickel layer, and a histidine tag peptide at the other end of the histidine tag peptide, Providing a biosensor having an antibody that binds to the target antigen and captures the target antigen;
In order to prevent the signal distortion of the biosensor and improve the durability of the nickel layer, a barrier layer for blocking the binding of the target antigen to the nickel layer is applied to the nickel layer to form a barrier layer step;
A first rinsing step of injecting into the nickel layer a separation solution which breaks bonds of the histidine and the nickel layer to separate the histidine tag peptide and the antibody from the nickel layer; And
And a second rinse step of injecting an acidic solution into the nickel layer to clean the separation solution,
Wherein the histidine tag peptide and the antibody are removed from the sensor head after the first rinsing step and the second rinsing step so that the sensor head can be regenerated.

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The method according to claim 1,
Wherein the separation solution in the first rinsing step comprises an immidazole solution.

The method according to claim 1,
Wherein the acid solution of the second rinsing step comprises an acetic acid solution.

The method according to claim 1,
The metal layer may include,
A silver (Ag) layer laminated on the optical fiber core or a double layer structure including an aluminum (Al) layer laminated on the gold layer; and an aluminum (Al Layer of the sensor head of the biosensor.