KR101223762B1 - Biosensor using bragg grating waveguide for surface plasmon and detection method for target material using the same - Google Patents
Biosensor using bragg grating waveguide for surface plasmon and detection method for target material using the same Download PDFInfo
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- KR101223762B1 KR101223762B1 KR1020100066227A KR20100066227A KR101223762B1 KR 101223762 B1 KR101223762 B1 KR 101223762B1 KR 1020100066227 A KR1020100066227 A KR 1020100066227A KR 20100066227 A KR20100066227 A KR 20100066227A KR 101223762 B1 KR101223762 B1 KR 101223762B1
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Abstract
The biosensor device using the surface plasmon Bragg grating waveguide of the present invention is a metal layer formed for the surface plasmon waveguide, a dielectric layer disposed in contact with the upper side of the metal layer, and an upper dielectric layer and a metal layer including the Bragg grating portion formed on the dielectric layer in the longitudinal reference region. The target material receptor may be coated on and attached to the lower dielectric layer and the Bragg grating portion disposed in contact with the lower side.
In the biosensor device and the target material detection method according to the present invention, a Bragg grating is generated in the waveguide through which the SPP is transmitted, and the target material is detected simply and effectively by measuring the SPP wavelength.
Description
The present invention relates to a biosensor and a detection method for detecting a target substance. In particular, the present invention relates to a biosensor and a method for detecting a target substance bound to a receptor by measuring a reflected wave generated in a surface plasmon polariton (SPP) Bragg grating waveguide.
It has recently been experimentally confirmed that light waves interact with free electrons on the metal surface and cause resonance when certain conditions are met at the boundary between metal and dielectric. This resonance corresponds to resonance between electromagnetic waves outside the metal and free electrons in the metal.
The result is Surface Plasmon, a traveling wave of dense electrons that resembles the shape of a wave flowing along a surface. Surface Plasmon (SP) or Surface Plasmon Polariton (SPP) is a light or photon that travels along the surface in the form of light or photons combined with plasma at the interface between the metal and the dielectric.
When the light waves incident on the interface between the metal and the dielectric with TM polarization (Transverse Magnetic Polarization) satisfy the phase matching conditions by an appropriate method, they create a collective movement of electrons (plasma) on the metal surface, and the combination causes the interface between the metal and the dielectric. Produces the near field of the jacket.
The magnitude of the electric field of these surface plasmon waves can be made from several nanometers to several tens of micrometers or more, and has the characteristics of strong local near field, distinctive dispersion and resonance phenomena (SPR).
Since the surface plasmons generated in this way are concentrated in the vicinity of the metal surface, they are frequently used to measure the properties of the materials present on the metal surface and the refractive index of the metal. In addition, since the shape, refractive index, and the like of the metal surface or the dielectric layer are sensitive to changes that may affect the surface plasmon wave that is in progress, the structure and the characteristics thereof may be used in applications such as sensors.
In addition, waveguides for surface plasmon waves are used for waveguide and modulation, and are widely applied to optical devices such as plasmon sources, receivers, dividers, and combiners.
Biosensors are devices or devices designed to detect and measure substances related to life phenomena, ranging from proteins such as DNA, antigens, and antibodies to cells, and are used for diagnosing diseases, developing new drugs, monitoring the environment, and food safety. It is applied in many fields. In recent years, development of a non-labeled biosensor, which is simple to prepare a sample, is more active than a conventional biosensor which detects a target substance by attaching a label such as radioisotope or fluorescent substance.
In particular, optical biosensors that detect optical characteristics changed by biochemical reactions such as antigen-antibody reactions occurring on the surface of a sensor such as a surface plasmon resonant biosensor, an optical waveguide biosensor, or an interferometric biosensor have been attracting attention.
Sensing methods of general optical biosensors include fluorescence method, absorption type, non-dispersive infrared absorption method, transmission light spectrum SPR bulk and optical waveguide type, dielectric optical waveguide type, and the optical module can be integrated and integrated. There is a waveguide type.
Dielectric optical waveguide type and SPR optical waveguide type have a problem of inferior measurement accuracy compared to SPR bulk type. In addition, the SPR bulk type is formed only on the surface of the metal thin film, and has a problem in that a short traveling distance and a complicated structure are required.
In the present invention, by forming a dielectric Bragg grating coated with a target material receptor on a surface plasmon polaritone waveguide made of a metal made of a very thin transparent electron ink, the detection limit of the material to be detected can be raised, and the optical modularization, integration and The present invention was completed by finding out that it can be manufactured at low cost.
The biosensor device and the target material detection method using the surface plasmon Bragg grating waveguide according to the present invention aims to solve the following problems.
First, the detection limit of a target material to be detected is increased by using a metal layer made of transparent electronic ink.
Second, a waveguide having a Bragg grating is used to detect whether the target material is coupled and / or the concentration of the target material through the wavelength of the reflected wave.
Third, it is possible to manufacture a micro biosensor device through optical modularization and integration using SPP Bragg grating.
Fourth, through the optical module and integration using SPP Bragg grating, it is possible to manufacture a high-efficiency biosensor device at low cost.
The solution to the problem of the present invention is not limited to those mentioned above, and other solutions not mentioned can be clearly understood by those skilled in the art from the following description.
The biosensor device using the surface plasmon Bragg grating waveguide for solving the above problems is a metal layer formed for the surface plasmon waveguide, a dielectric layer disposed in contact with the upper side of the metal layer, the upper portion including the Bragg grating portion formed on the dielectric layer in the longitudinal reference region range And a receptor portion coated with a target material receptor on the dielectric layer, the lower dielectric layer disposed under the metal layer, and the Bragg grating portion.
The biosensor device using the surface plasmon Bragg grating waveguide according to another aspect of the present invention is a metal layer formed for the surface plasmon waveguide, the metal layer including the Bragg grating portion formed in the metal layer in the longitudinal reference region range, the upper portion disposed in contact with the upper metal layer The dielectric layer, the lower dielectric layer disposed under the metal layer, and the receptor portion coated with the target material receptor on the upper dielectric layer in the longitudinal reference region range of the metal layer.
The Bragg grating portion of the metal layer according to the present invention is characterized in that the Bragg grating is formed to protrude on either side of the upper or lower side of the metal layer.
The Bragg grating portion of the metal layer according to the present invention includes that the Bragg grating is formed to protrude on either side of the left or right side of the metal layer.
According to another aspect of the present invention, a biosensor device using a surface plasmon Bragg grating waveguide includes a metal layer formed for a surface plasmon waveguide, an upper dielectric layer disposed in contact with an upper side of the metal layer, a lower dielectric layer disposed in contact with a lower side of the metal layer, and an upper dielectric layer in a longitudinal direction A receptor portion coated with a target material receptor on an upper dielectric layer in a region range, wherein the receptor portion is a receptor portion having different heights or a receptor portion having the same height is alternately arranged with reference intervals. And a receiver portion spaced apart and spaced apart.
According to another aspect of the present invention, a biosensor device using a surface plasmon Bragg grating waveguide includes a metal layer formed for a surface plasmon waveguide, an upper dielectric layer disposed in contact with an upper side of the metal layer, a lower dielectric layer disposed in contact with a lower side of the metal layer, and an upper dielectric layer in a longitudinal direction The target material receptor is coated on the upper dielectric layer in the region range, including a receptor portion is attached, wherein the metal layer is characterized in that disposed in the form spaced apart at a reference interval in the upper dielectric layer longitudinal reference region range.
The metal layer according to the present invention is characterized in that it is formed of any one of a precious metal or a transition metal.
The metal layer according to the present invention includes one formed of one selected from the group consisting of gold (Au), silver (Ag), copper (Cu), aluminum (Al), and alloys thereof.
The metal layer has a thickness of 5 nm to 50 nm and a width of 1 μm to 100 μm.
The dielectric layer according to the present invention is characterized in that it is made of any one material of silicon (Si), quartz (SiO 2 ) or polymer (Polymer).
Target substance receptors according to the present invention comprise one or more of antigens, antibodies, enzymes, proteins, rapton, DNA, RNA, cells, hormone receptors, biofilms or tissues.
The length of the longitudinal reference region range according to the invention is characterized in that 10μm to 10mm.
In the Bragg grating portion according to the present invention is characterized in that the regular cross-sectional shape of the grating is at least one of square, rectangular, triangular or semi-circular.
The grid spacing Λ g of the Bragg grating portion according to the present invention is characterized by the following equation (1).
The reference spacing of the receptor end according to the invention is characterized in that the same as the lattice spacing of the Bragg grating portion.
The method for detecting a target substance using the surface plasmon grating waveguide according to the present invention includes a step S1 in which an optical signal is input to an input terminal of a biosensor and the optical signal is converted into surface plasmon polaritone (SPP).
The target material detection method using the surface Plasmon lattice waveguide according to the present invention includes an S2 step in which the SPP converted in step S1 is transmitted through the Bragg lattice waveguide coated with the target material receptor.
The target material detection method using the surface plammonic lattice waveguide according to the present invention includes a step S3 in which at least one of a transmission wavelength or a reflection wavelength generated while the SPP passes through the lattice waveguide is measured in step S2.
The target material detection method using the surface plasmon lattice waveguide according to the present invention includes a step S4 by analyzing the wavelength measured in step S3 to calculate one or more of the presence or absence of the binding of the target material or the concentration of the bound material.
Bragg lattice waveguide of step S2 according to the present invention is a dielectric layer disposed in contact with the metal layer formed for the surface plasmon waveguide, the upper layer, the upper dielectric layer including the Bragg lattice formed on the dielectric layer in the longitudinal reference region range, the contact below the metal layer And a receptor portion coated with a target material receptor on the disposed lower dielectric layer and the Bragg grating portion.
Bragg grating waveguide of step S2 according to another aspect of the present invention, a metal layer formed for the surface plasmon waveguide, the metal layer including the Bragg grating portion formed in the metal layer in the longitudinal reference region range, the upper dielectric layer, the upper dielectric layer disposed on the upper metal layer, the lower metal layer And a receptor portion coated with a target material receptor on the upper dielectric layer in the longitudinal reference region range of the lower dielectric layer and the metal layer disposed in contact with the lower dielectric layer.
The Bragg grating portion of the metal layer used in the present invention is characterized in that the Bragg grating is formed to protrude on either side of the upper or lower side of the metal layer.
The Bragg grating portion of the metal layer used in the present invention is characterized in that the Bragg grating is formed to protrude on either side of the left or right side of the metal layer.
Bragg lattice waveguide of step S2 according to another aspect of the present invention, the upper portion in the metal layer formed for the surface plasmon waveguide, the upper dielectric layer disposed in contact with the upper metal layer, the lower dielectric layer disposed in contact with the lower metal layer and the upper dielectric layer longitudinal reference region range A receptor portion coated with a target material receptor coated on a dielectric layer, wherein the receptor portions are spaced apart with reference intervals or that the receptor portions having the same height are alternately arranged in order with the receiver ends having different heights spaced apart from each other. Characterized in that the receptor portion disposed in the form.
Bragg lattice waveguide of step S2 according to another aspect of the present invention, the upper portion in the metal layer formed for the surface plasmon waveguide, the upper dielectric layer disposed in contact with the upper metal layer, the lower dielectric layer disposed in contact with the lower metal layer and the upper dielectric layer longitudinal reference region range The target material receptor is coated on the dielectric layer to include a receptor portion, wherein the metal layer is characterized in that disposed in the form spaced apart at a reference interval in the upper dielectric layer longitudinal reference region range.
The step S4 according to the present invention is performed by comparing and analyzing the wavelength measured in step S3 with a reference wavelength which is one or more of a transmission wavelength or a reflection wavelength generated when no material is bound to the target material receptor.
The biosensor device and the target material detection method according to the present invention include a very thin metal layer formed of transparent electronic ink, so that the detection efficiency and the limit of the target material are high.
In the biosensor device and the target material detection method according to the present invention, a Bragg grating is generated in the waveguide through which the SPP is transmitted, and the target material is detected simply and effectively by measuring the SPP wavelength.
The biosensor device according to the present invention coats a target material receptor on a surface plasmon polaritone Bragg lattice waveguide including a very thin metal layer formed of transparent electronic ink, thereby making it very compact, low power consumption, and can be optically modularized and integrated. Provide a sensor.
The effects of the present invention are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.
1 (a) to 1 (c) respectively show an exploded perspective view, a cross-sectional view, and an enlarged view of a Bragg grating portion coupled to a receptor for a biosensor device using a surface plasmon Bragg grating waveguide according to an embodiment of the present invention.
2 (a) to 2 (c) respectively show an exploded perspective view, a cross-sectional view, and an enlarged view of a Bragg grating portion coupled to a receptor for a biosensor device according to another embodiment of the present invention.
3 (a) to 3 (e) show a form of Bragg grating which can be formed in a metal layer as another embodiment of the present invention.
4 (a) and 4 (b) show the shape of the receptor coated on the upper dielectric as another embodiment of the present invention.
FIG. 5 illustrates another embodiment of the present invention in which the metal layer optical waveguides are arranged in a form of periodically shorted.
FIG. 6 shows a schematic flowchart of a biomaterial detection method using a surface plasmon Bragg grating waveguide according to the present invention.
Figure 7 (a) shows the magnetic field distribution when there is no substance bound to the receptor, Figure 7 (b) shows the initial reference input light power distribution, Figure 7 (c) shows the SPP transmission optical power distribution, Figure 7 (d) shows the SPP reflected optical power distribution.
FIG. 8 (a) shows the magnetic field distribution when the target substance is bound to the target substance receptor, FIG. 8 (b) shows the input light power distribution when the target substance is bound, and FIG. 8 (c) shows the target. The SPP transmission optical power distribution when the materials are combined is shown, and FIG. 8 (d) shows the SPP reflected optical power distribution when the target materials are combined.
FIG. 9 (a) shows the initial reference wavelength and the shifted wavelength for the transmission wavelength and the reflection wavelength, and FIG. 9 (b) shows the initial reference wavelength and the shifted wavelength (Δλ) for the transmission wavelength in comparison. .
Hereinafter, a biosensor device and a target material detection method using the surface plasmon Bragg grating waveguide according to the present invention will be described in detail with reference to the accompanying drawings.
1 is an exploded perspective view, a cross-sectional view, and an enlarged view of a receptor coupled to a biosensor device using a surface plasmon Bragg grating waveguide according to an embodiment of the present invention.
The biosensor device using the surface plasmon Bragg grating waveguide according to the present invention is basically a
Although not shown in FIG. 1, when an optical signal is output from a light source, the optical signal is converted into a surface plasmon polaritone (SPP) signal and then transmitted through a metal waveguide.
The
Transparent electronic ink (Transparent electronic ink or Transparent silver ink) is a transparent ink containing a metal component. Transparent electronic ink is used to form a thin metal layer containing a metal component on an object of various materials by outputting a printed matter in a device such as a printer.
After all, the
The
It is preferable that the thickness of the
The dielectric layer 200 of the present invention has a form disposed in contact with the upper side and the lower side of the
Since the present invention detects the binding of the target material by measuring the SPP wavelength, unlike the conventional biosensor, the lower
Substrate configurations commonly used in biosensors are well known to those skilled in the art, and a detailed description thereof will be omitted.
The dielectric is an insulator through which no current flows, and the dielectric layer is preferably made of any one of silicon nitride (Si 3 N 4 ), quartz (SiO 2 ), or polymer.
The target substance receptor is the site to which the target substance which the biosensor wants to measure binds. As the target material, biological materials such as antigens, antibodies, DNA, RNA, and proteins produced when a specific disease occurs, are usually used, but the present invention is not limited thereto, and a chemical material may be the target material.
The target substance receptor is one that selectively recognizes and binds the target substance. Only specific target substances can be bound through specific protein structures. Receptors are usually biomaterials such as antigens, antibodies, enzymes, proteins, raptons, DNA, RNA, cells, hormone receptors, biofilms or tissues.
Bragg grid Waveguide
Hereinafter, a structure for forming a Bragg grating waveguide will be described in detail. The Bragg grating is formed in the form and combined form of each of the
The Bragg grating, which is one of the key components of the present invention, causes the SPP to be transmitted through the metal waveguide to produce a specific reflected wave. This creates a specific transmission wavelength as described below.
As an embodiment of the present invention, the biosensor device using the surface plasmon Bragg grating waveguide of the present invention, as shown in Figure 1 (a) to Figure 1 (c) is a
The Bragg grating is created on the
The sensitivity of the biosensor can be adjusted according to the distance between the dielectric Bragg grating portion and the waveguide metal layer. The sensitivity increases as the distance between the dielectric Bragg grating portion and the waveguide metal layer decreases.
The refractive index of the dielectric must be lower than the refractive index of 1.334 in saline solution to act as a Bragg grating. Therefore, it is desirable to have a refractive index of 1.33 or less.
As another embodiment of the present invention, as shown in Figs. 2 (a) to 2 (c), the
The Bragg grating is formed on the
3 (a) to 3 (e) illustrate the form of Bragg gratings that can be formed in the
In an exemplary embodiment, the Bragg grating 150 of the
In another embodiment, the Bragg grating portion of the
Although it is assumed that the Bragg grating is formed on any one of the
In the Bragg
The lattice spacing Λ g of the
The Bragg operating wavelength λ B is the traveling wavelength of the SPP, and the effective refractive index n eff means the effective refractive index of the portion where the Bragg grating is formed. Equation 1 is a Bragg equation showing the correlation between the lattice spacing, Bragg operating wavelength (λ B ) and the effective refractive index. The grating spacing is determined by dividing the effective refractive index of the waveguide by half of the wavelength to be reflected. This is the principle of Fresnel reflection, that is, when the irregularities of the periodic effective refractive index are the working wavelength divided by the effective refractive index, and half the length of the wavelength propagated in the actual waveguide, the light reflects the change of the refractive index. When the reflected light differs by half the wavelength, constructive interference occurs between the reflected lights, and proceeds in the opposite direction of the traveling direction. The wavelength at this time is called Bragg operating wavelength. Light outside of the Bragg operating wavelength will proceed.
The length of the longitudinal reference region range is the length of the Bragg
The Bragg grating itself is preferably formed in the
4 (a) to 4 (b) show the shape of the receptor coated on the upper dielectric as another embodiment of the present invention.
As another embodiment of the present invention, the biosensor device using the surface plasmon Bragg grating waveguide is basically a
Alternating arrangement of receptor ends with different heights can have various embodiments. Assuming that there are acceptor groups A, B and C, each of different lengths, they may be arranged in the form of ABCABCABC or in the same order as ACBACBACBACB.
After all, various combinations are possible as long as they have a uniform order and a uniform shape as a whole. Most preferably, the arrangement of the receptor ends has a shape similar to that of Bragg lattice.
4 (a) is limited to two receptor ends, and shows that the first and second receptor ends having a constant length are alternately arranged. For example, it corresponds to the form arranged in ABABABAB order.
In an embodiment in which receptor groups capable of sensing different materials are alternately arranged, two receptor groups having the same height may be used. Alternatively, the height of the receptor stage used may vary slightly.
Refractive indexes of the first and second receptor ends coupled to the target material are different from each other, and the first and second receptor ends are alternately arranged. As a result, a difference in refractive index between the first receptor end coupled to the first target material and the second receptor end coupled to the second target material may occur to form a lattice shape with respect to the refractive index.
As another embodiment, as shown in Figure 4 (b), the receptor end having the same height may be spaced apart at a constant reference interval. This case also has a form similar to the Bragg grating.
FIG. 5 illustrates another embodiment of the present invention in which the optical waveguide of the
As another embodiment of the present invention, the biosensor device using the surface plasmon Bragg grating waveguide is a
The metal layers 100 are spaced apart at regular intervals in a length region corresponding to the reference region range of the
The length of one receptor end, the separation distance of the receptor end, and the separation distance of the
Therefore, the reference interval may be expressed in the same manner as in Equation 1 described above. The equation of reference spacing = lattice spacing Λ g is established.
surface Plasmon Bragg grid Waveguide Used target Substance detection method
Hereinafter, a method of detecting a target substance using the surface plasmon lattice waveguide will be described in detail. However, parts common to the biosensor device using the surface plasmon Bragg grating waveguide will be omitted, and the description will be mainly focused on the core configuration of the detection method.
FIG. 6 shows a schematic flowchart of a biomaterial detection method using a surface plasmon Bragg grating waveguide according to the present invention.
In the target material detection method using the surface plasmon lattice waveguide according to the present invention, the optical signal is input to the input terminal of the biosensor, and the SPP converted in the step S1 and S1 is converted into the surface plasmonic polaritone (SPP). By analyzing the wavelengths measured in steps S3 and S3 where the target operating material receptor is transmitted through the coated lattice waveguide, and the Bragg operating wavelengths generated as the SPP passes through the lattice waveguide in S2, S4 step of calculating at least one of the presence or absence of bound substance.
In step S1, an optical signal from a light source is converted into an SPP signal. This process is a general step for using the SPP signal, so a detailed description thereof will be omitted.
One of the key components of the detection method of the present invention is the step S2. This is because the target material is bound to the receptor in the form of the wavelength of the transmitted wave that appears as the SPP is transmitted through the Bragg grating waveguide.
The Bragg grating waveguide has the same configuration as the biosensor element described above. In other words, a Bragg grating is formed on the
The Bragg grating waveguide of step S2 is a dielectric layer disposed in contact with the
In another embodiment, the Bragg grating waveguide of step S2 is a
The Bragg grating 150 of the
As another example, the Bragg grating 150 of the
As another embodiment of the present invention, the Bragg grating waveguide of step S2 is disposed in contact with the
As another embodiment of the present invention, the Bragg grating waveguide of step S2 is disposed in contact with the
As the SPP signal passes through the Bragg grating waveguide, the Bragg grating causes a specific transmitted and reflected wavelength at the Bragg operating wavelength. Since the reflected wave is generated at a certain Bragg wavelength due to the Bragg grating, the Bragg operating wavelength region of the transmitted wave wavelength is recessed as shown in FIG. 7 (c), and since the wave of the recessed portion is reflected, FIG. 7 (d). It has the same reflected wave characteristics.
When the target material is bound to the target material receptor, the refractive index of the bonded portion is changed, thereby changing the effective refractive index n eff in the vertical direction, thereby changing the SPP transmission wave and the reflected Bragg operating wavelength λ B. The change in wavelength Δλ B may be measured to detect whether the target substance is bound to the target substance receptor.
In step S3, at least one of a transmission wavelength generated while the SPP passes through the grating waveguide or a reflection wavelength generated when the SPP is reflected may be measured.
Step S4 is performed by comparing and comparing the wavelength measured in step S3 with a reference wavelength, which is a transmission and / or reflected wave Bragg operating wavelength generated when no material is bound to the target material receptor.
When no substance is bound to the receptor, it has the same shape as the transmission wave wavelength shown in FIG. 7 (b) or the reflection wave wavelength shown in FIG. 7 (c). The wavelength at this time is called an initial reference wavelength. When the target material is bound to the receptor, the wavelength is shifted as shown in FIG. 8 (b) or 8 (c). It can be seen that the recessed area caused by the reflected wave moved to the right side.
As a reference wavelength, a transmission wave wavelength may be used, or a reflected wave wavelength may be used. Furthermore, both the transmission wave wavelength and the reflection wave wavelength may be used.
In FIG. 7 and FIG. 8, the x axis represents wavelength and the y axis represents optical power representing intensity of the wavelength.
FIG. 9 shows the distance (Δλ) shifted relative to the initial reference wavelength when the biomaterial binds to the receptor. FIG. 9 (a) shows the initial reference wavelength and the shifted wavelength for the transmission wavelength and the reflection wavelength, and FIG. 9 (b) compares the initial reference wavelength and the shifted wavelength for the transmission wave.
If Δλ is greater than or equal to a certain size, it can be seen that the target substance is bound to the target substance receptor. Through this, the binding of the target material can be confirmed.
Furthermore, the concentration of the bound substance can be determined by measuring in advance the size of Δλ according to the bound amount of the target substance. Depending on the concentration of the bound substance can be detected in several steps, or compared to the measurement graph that previously measured the degree of movement in accordance with the concentration of the substance can be accurately measured the concentration of the substance bound to the receptor.
The above-described biosensor or target material detection method has been described on the premise that the
Since the present invention detects the target material by measuring a wavelength rather than checking whether the target material is detected through an electrical signal, the substrate does not necessarily need to be disposed in contact with the lower
The embodiments and drawings attached to this specification are merely to clearly show some of the technical ideas included in the present invention, and those skilled in the art can easily infer within the scope of the technical ideas included in the specification and drawings of the present invention. Modifications that can be made and specific embodiments will be apparent that all fall within the scope of the present invention.
100: metal layer 150: Bragg grating portion of the
200: dielectric layer 210: upper dielectric layer
220: lower dielectric layer 250: Bragg grating portion of the upper dielectric layer
300: receptor portion
Claims (32)
A metal layer formed for the surface plasmon waveguide;
A dielectric layer disposed on and in contact with the metal layer, the upper dielectric layer including a Bragg grating portion formed on the dielectric layer in a longitudinal reference region;
A lower dielectric layer disposed below and in contact with the metal layer; And
A biosensor device using a surface plasmon Bragg grating waveguide, characterized in that it comprises a receptor portion coated with a target material receptor coated on the Bragg grating portion.
A metal layer formed for a surface plasmon waveguide, the metal layer comprising a Bragg grating portion formed in the metal layer in a longitudinal reference region;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
The biosensor device using the surface plasmon Bragg grating waveguide, characterized in that it comprises a receptor portion coated with a target material receptor on the upper dielectric layer in the longitudinal reference region of the metal layer.
The Bragg grating portion of the metal layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that the Bragg grating is formed to protrude on at least one side of the upper or lower side of the metal layer.
The Bragg grating portion of the metal layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that the Bragg grating is formed to protrude on at least one side of the left or right side of the metal layer.
A metal layer formed for the surface plasmon waveguide;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
Including the receptor portion is coated with a target material receptor on the upper dielectric layer in the upper dielectric layer longitudinal reference region range,
The receptor portion is a surface plasmon Bragg grating, characterized in that the receptor ends having different heights are arranged in alternating order and the receptor ends having the same height or the receptor ends are arranged in a spaced apart form with reference spacing Biosensor device using waveguide.
A metal layer formed for the surface plasmon waveguide;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
Including the receptor portion is coated with a target material receptor on the upper dielectric layer in the upper dielectric layer longitudinal reference region range,
The metal layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that disposed in the form of spaced apart at a reference interval in the longitudinal reference region range of the upper dielectric layer.
The metal layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that formed of any one of a precious metal or a transition metal.
The metal layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that formed of any one selected from the group consisting of gold (Au), silver (Ag), copper (Cu), aluminum (Al) and alloys thereof.
The thickness of the metal layer is 5 nm to 50 nm, the width of the biosensor device using a surface plasmon Bragg grating waveguide, characterized in that 1μm to 100μm.
The dielectric layer is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that the material is made of any one of silicon (Si), quartz (SiO 2 ) or polymer (Polymer).
The target material receptor is a biosensor device using a surface plasmon Bragg grating waveguide, characterized in that it comprises one or more of antigen, antibody, enzyme, protein, racton, DNA, RNA, cell, hormone receptor, biofilm or tissue.
The biosensor device using the surface plasmon Bragg grating waveguide, characterized in that the length of the longitudinal reference region range is 10μm to 10mm.
The biosensor device using the surface plasmon Bragg grating waveguide, characterized in that the regular cross-sectional shape of the grating in the Bragg grating portion is at least one of square, rectangular, triangular or semi-circular.
The lattice spacing (Λ g ) of the Bragg grating portion is determined by the following equation biosensor device using a surface plasmon Bragg grating waveguide.
The reference interval is a biosensor device using a surface plasmon Bragg grating waveguide, characterized by the following equation.
An optical signal is input to an input terminal of the biosensor, and the optical signal is converted into surface plasmon polaritone (SPP);
S2 step of transmitting the SPP converted in the step S1 through the Bragg grating waveguide coated with the target material receptor;
Step S3 of measuring at least one of a transmission wavelength or a reflection wavelength generated while the SPP passes through the grating waveguide in step S2; And
Analyzing the wavelength measured in the step S3, the target material detection method using the surface plasmon grating waveguide comprising the step S4 of calculating at least one of the presence or absence of the binding of the target material or the concentration of the bound material.
The Bragg grating waveguide of step S2
A metal layer formed for the surface plasmon waveguide;
A dielectric layer disposed on and in contact with the metal layer, the upper dielectric layer including a Bragg grating portion formed on the dielectric layer in a longitudinal reference region;
A lower dielectric layer disposed below and in contact with the metal layer; And
A target material detection method using a surface plammonic lattice waveguide, characterized in that it comprises a receptor portion coated with a target material receptor coated on the Bragg grating portion.
The Bragg grating waveguide of step S2
A metal layer formed for a surface plasmon waveguide, the metal layer comprising a Bragg grating portion formed in the metal layer in a longitudinal reference region;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
The target material detection method using a surface plasmon grating waveguide, characterized in that it comprises a receptor portion coated with a target material receptor on the upper dielectric layer in the longitudinal reference region range of the metal layer.
The Bragg grating portion of the metal layer is a target material detection method using a surface Plasmon grating waveguide, characterized in that the Bragg grating is formed to protrude on at least one side of the upper or lower side of the metal layer.
The Bragg grating portion of the metal layer is a target material detection method using a surface plazione grating waveguide, characterized in that the Bragg grating is formed to protrude on at least one of the left or right side of the metal layer.
The Bragg grating waveguide of step S2
A metal layer formed for the surface plasmon waveguide;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
Including the receptor portion is coated with a target material receptor on the upper dielectric layer in the upper dielectric layer longitudinal reference region range,
The receptor portion is characterized in that the receptor ends having different heights are arranged in alternating order and the receptor portion having the same height or the receptor portion is arranged in a spaced apart form having a reference interval, the surface plammonic lattice Target material detection method using a waveguide.
The Bragg grating waveguide of step S2
A metal layer formed for the surface plasmon waveguide;
An upper dielectric layer disposed in contact with the metal layer;
A lower dielectric layer disposed below and in contact with the metal layer; And
Including the receptor portion is coated with a target material receptor on the upper dielectric layer in the upper dielectric layer longitudinal reference region range,
The metal layer is a target material detection method using a surface plyjimon grating waveguide, characterized in that the spaced apart spaced apart from the reference region in the longitudinal reference region range of the upper dielectric layer.
The metal layer is a target material detection method using a surface plasmon grating waveguide, characterized in that formed of any one selected from the group consisting of gold (Au), silver (Ag), copper (Cu), aluminum (Al) and alloys thereof.
The target layer detection method using the surface plasmon Bragg grating waveguide, characterized in that the metal layer formed for the surface plasmon waveguide is made of any one of a precious metal or a transition metal.
The metal layer has a thickness of 5 nm to 50 nm and a width of 1μm to 100μm Target material detection method using a surface plasmon Bragg grating waveguide.
The dielectric layer is a target material detection method using a surface plasmon Bragg grating waveguide, characterized in that made of any one material of silicon (Si), quartz (SiO 2 ) or polymer (Polymer).
The target material receptor is a target material detection method using a surface plasmon Bragg lattice waveguide, characterized in that it comprises one or more of an antigen, an antibody, an enzyme, a protein, racton, DNA, RNA, cells, hormone receptors, biofilm or tissue.
The length of the longitudinal reference region range is 10μm to 10mm Target material detection method using a surface plasmon Bragg grating waveguide.
The target cross-sectional shape of the grating in the Bragg grating unit is a target material detection method using a surface plasmon Bragg grating waveguide, characterized in that at least one of square, rectangular, triangular or semi-circular.
The lattice spacing (Λ g ) of the Bragg grating portion) is determined by the following equation.
The reference interval is a target material detection method using a surface plasmon Bragg grating waveguide, characterized in that the equation below.
The step S4
Target material detection using a surface plasmon Bragg grating waveguide, which is performed by comparing and analyzing a wavelength measured in step S3 with a reference wavelength that is one or more of a transmission wavelength or a reflection wavelength generated when no material is bound to a target material receptor. Way.
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| KR20080083922A (en) * | 2007-03-14 | 2008-09-19 | 한양대학교 산학협력단 | Long range surface plasmon optical waveguide sensors having double metal layers |
| KR100922578B1 (en) * | 2007-12-17 | 2009-10-21 | 한국전자통신연구원 | Disposable surface plasmon resonance biosensor and system using the same |
| KR20100068179A (en) * | 2008-12-12 | 2010-06-22 | 한국전자통신연구원 | Bio-sensor for measuring magnetoresistive and method for manufacturing the same |
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| KR100922578B1 (en) * | 2007-12-17 | 2009-10-21 | 한국전자통신연구원 | Disposable surface plasmon resonance biosensor and system using the same |
| KR20100068179A (en) * | 2008-12-12 | 2010-06-22 | 한국전자통신연구원 | Bio-sensor for measuring magnetoresistive and method for manufacturing the same |
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| US9865246B2 (en) | 2013-11-11 | 2018-01-09 | Samsung Electronics Co., Ltd. | Laser-induced ultrasound generator and method of manufacturing the same |
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