KR102089588B1 - Broccoli sprouts buds increased growth rate and sulforaphane content, and method for producing thereof - Google Patents

Abstract

The present invention relates to a broccoli sprout with increased growth rate and sulforaphane content, and a method for producing the same. (A) Germinating broccoli seeds under water containing lava seawater; (B) supplying the water containing the lava seawater to the germinated broccoli seeds; by including, it can significantly improve the content of sulforaphane compared to the same amount of raw materials and improve yield by increasing the growth rate of broccoli sprouts.

Description

Broccoli sprouts buds increased growth rate and sulforaphane content, and method for producing thereof}

The present invention relates to a broccoli sprout with increased content and growth rate of sulforaphane compared to the same raw material amount and a method for producing the same.

A sprout vegetable is a plant shoot that generally refers to 2 to 7 days after germination.

The sprout vegetable has a great attention in that it is a non-polluting food that perfectly contains essential nutrients that life needs, and has no stress caused by pests and no contamination of pesticides or chemical fertilizers.

When all the seeds are germinated, all nutrients inside the seeds are awakened and supplied to the buds, and the nutrients are easily changed so that the buds can grow at the highest growth rate, so the plants are most vigorous at the time of sprouting.

Seeds that are prepared to emerge from thick embryos sprout when given moisture and temperature, where the plants synthesize new substances as weapons to defend themselves from external enemies, such as mold and bacteria.

In other words, it produces enzymes, vitamins, amino acids, and other substances that were not present in the seed state, but these useful substances are maximized when the buds are in the 2nd to 7th day, that is, in the young cotyledon state before the leaves appear.

The useful substances of sprouts that are many days after germination are up to 20 to 100 times more than 2 to 3 months later. In the case of broccoli, which has been spotlighted as a food that can prevent aging and disease in recent years, 20% of adults when ingesting sprouts A sufficient effect can be seen by ingesting a small amount of about 1-50.

The broccoli is one of cruciferous vegetables containing a large amount of Sulforaphane, which is known to inhibit inflammatory trigger activity in addition to the anti-cancer and Helicobacter pylori inhibitory effect. have.

More specifically, sulforaphane is a type of isocyanate, and is a substance formed by hydrolysis of glucosinolate by myrocinase during digestion. Glutathione is synthesized in the human body to remove peroxide, thereby preventing skin whitening and skin cancer. It has the role of reducing the anti-cancer effect and Helicobacter bacterial proliferation by inhibiting the expression of genes that cause oxidative stress.

In addition, broccoli sprouts are rich in vitamins A, B, C, K, potassium, sulfur, chlorine, and selenium, helping blood circulation and bone health, while indole compounds in broccoli sprouts are estrogen It is known to inhibit the growth and metastasis of breast cancer cells that are sensitive to.

Therefore, there is a need for a technique capable of increasing the content of sulfolapan contained in broccoli sprouts while improving the growth rate of broccoli sprouts with such excellent efficacy.

Republic of Korea Registered Patent No. 10-1321795 Republic of Korea Registered Patent No. 10-1314885

An object of the present invention is to provide a method for producing broccoli sprouts having an increased content and growth rate of sulforaphane compared to the same raw material amount.

In addition, another object of the present invention is to provide a broccoli sprout having an increased content and growth rate of sulforaphane compared to the same raw material amount.

The method for producing broccoli sprouts with increased growth rate and sulfolapan content of the present invention for achieving the above object includes: (A) germinating broccoli seeds under water containing lava seawater; (B) supplying the water containing the lava seawater to the germinated broccoli seeds.

After the step (B) (C) after the broccoli sprouts grown with water containing the lava seawater are introduced into a batch or continuous processing vessel, an out voltage of 50 to 80%, a pulse width of 15 to 35 μs, The method may further include performing a high voltage pulse electric field treatment for 1 to 10 seconds so that the total energy is 1 to 30 kJ at a frequency of 50 to 200 Hz.

The concentration of the lava seawater may be 300 to 3000 ppm.

The electrical conductivity of the lava seawater may be 5 to 30 mS / cm.

The lava seawater may include 0.01 to 0.03 ppm of zinc, 0.01 to 0.03 ppm of iron, 0.006 to 0.02 ppm of manganese, 0.01 to 0.03 ppm of vanadium, 0.01 to 0.03 ppm of selenium, and 0.001 to 0.005 ppm of germanium. .

The batch or continuous processing vessel may be provided with a plurality of electrodes at intervals of 1 to 80 mm.

In addition, the broccoli sprouts with increased growth rate and sulfolapan content of the present invention for achieving the above-mentioned other object may be germinated and grown broccoli seeds with water containing lava seawater.

After the broccoli sprouts grown with the water containing the lava seawater are introduced into a batch or continuous processing vessel, the total energy is used by using an out voltage of 50 to 80%, a pulse width of 15 to 35 μs, and a frequency of 50 to 200 Hz. It may be a high voltage pulse electric field treatment for 1 to 10 seconds to be 1 to 30 kJ.

The broccoli sprouts with increased growth rate and sulforaphane content of the present invention can significantly improve the content of sulfolapan compared to the same raw material amount, and at the same time, improve yield by increasing the growth rate of broccoli sprouts.

In addition, since the broccoli sprout of the present invention has a high content of sulforaphane, it is effective in improving anti-inflammatory, anti-cancer, cognitive, and memory capabilities, and thus can be used as a pharmaceutical composition or a food composition capable of improving, preventing or treating it.

The present invention relates to a broccoli sprout with increased content and growth rate of sulforaphane compared to the same raw material and a method for producing the same.

Hereinafter, the present invention will be described in detail.

The method for producing broccoli sprouts with increased growth rate and sulfolapan content of the present invention includes (A) germinating broccoli seeds under water containing lava seawater; (B) supplying the water containing the lava seawater to the germinated broccoli seeds; includes; (C) After the broccoli sprouts grown with the lava seawater-containing water are put into a batch or continuous processing vessel, the total voltage is 50 to 80%, the pulse width is 15 to 35 us, and the frequency is 50 to 200 Hz. The method may further include performing a high voltage pulse electric field treatment for 1 to 10 seconds so that the energy is 1 to 30 kJ.

First, in step (A), broccoli seeds are germinated under water containing lava seawater.

Using water containing lava seawater as the water required to germinate broccoli seeds may increase the growth rate of broccoli sprouts and the content of sulforaphane than using tap water, ordinary seawater, or deep ocean water.

The concentration of the lava seawater is 300 to 3000 ppm, preferably 500 to 2000 ppm, more preferably 800 to 1300 ppm. When the concentration of lava seawater is less than the lower limit, the growth rate is not significantly high, and the sulforaphane content may not increase even after treatment with a high-voltage pulse electric field. If the concentration exceeds the upper limit, the growth rate may decrease.

In addition, the lava seawater of the present invention is electrodialyzed to have an electrical conductivity of 5 to 30 mS / cm, preferably 10 to 15 mS / cm. When the electrical conductivity is less than the lower limit, the growth rate of the broccoli sprout may not increase, and when it exceeds the upper limit, the broccoli sprout may not grow and the content of sulforaphane may not increase.

Such lava seawater is a saltwater sewage existing in the eastern part of Jeju Island, and is a unique water resource that exists only in Jeju Island, which is always filtered and filtered by basalt and contains clean and useful minerals. The lava seawater contains a lot of useful minerals such as vanadium, selenium, and germanium compared to tap water, general seawater, and deep ocean water. Specifically, zinc is 0.01 to 0.03 ppm, iron is 0.01 to 0.03 ppm, and manganese is 0.006. To 0.02 ppm, vanadium to 0.01 to 0.03 ppm, selenium to 0.01 to 0.03 ppm and germanium to 0.001 to 0.005 ppm.

In step (A), it is preferable to stand for 2 days in dark conditions in order to germinate broccoli seeds. When germination is performed under light conditions other than the dark conditions, the growth rate of broccoli sprouts and the increase in sulforaphane content may be insignificant.

Next, in step (B), the sprouted broccoli seeds are supplied with water containing the lava seawater to grow into broccoli sprouts.

After the broccoli seeds germinate, a light source is irradiated for 4 to 5 days to grow into broccoli sprouts, and the light source is not particularly limited as long as it can grow the germinated broccoli seeds into broccoli sprouts.

Next, in step (C), the grown broccoli sprouts are introduced into a batch or continuous processing container, and then a high voltage pulse electric field (PEF) treatment is performed for 1 to 10 minutes to further amplify the content of sulfolapan in the broccoli sprouts.

The high-voltage pulse electric field technology (PEF) of the present invention is a non-heating treatment technology that selectively collapses the cell membrane at the moment when the potential difference between the cell membranes generated by processing a sample at a high voltage of 1 to 87 kV / cm in microsecond units exceeds 1 V. to be.

The conditions of the high voltage pulse electric field used in the present invention are 50 to 80%, preferably 60 to 70% out voltage (out voltage); A pulse width of 15 to 35 μs, preferably 25 to 30 μs; Frequencies between 50 and 200 Hz, preferably between 100 and 120 Hz; A total energy of 1 to 30 kJ, preferably 5 to 8 kJ; 1 to 10 seconds, preferably 2 to 5 seconds.

When the high voltage pulse electric field is processed, if the out voltage, frequency, pulse width, and / or energy are less than the lower limit, the content of sulfolapan of the broccoli sprout may not be amplified, and if it exceeds the upper limit, the tissue of the broccoli sprout is destroyed and the component Change may appear.

The batch or continuous processing vessel in which the broccoli sprouts grown in the step (B) are introduced are provided with 5 to 30 kW class pulse generators and electrodes for high voltage pulse electric field (PEF) treatment, wherein the electrodes are 50 to 200 mm, It is preferably provided at 2 to 15 pieces at intervals of 60 to 100 mm.

When the gap between the electrodes is less than the lower limit, the content of sulfolapan in the broccoli sprout may not be amplified, and when the electrode is over the upper limit, the tissue of the broccoli sprout is destroyed and the content of sulfolapan is rather reduced and component changes may occur.

The broccoli sprout of the present invention has a high content of sulforaphane and is effective in improving cognitive ability and memory, so it can be used as a pharmaceutical composition or a food composition capable of improving, preventing or treating it.

Hereinafter, a preferred embodiment is provided to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it is apparent to those skilled in the art that various changes and modifications are possible within the scope and technical scope of the present invention. It is no wonder that such variations and modifications fall within the scope of the appended claims.

[By lava seawater concentration]

Control. Use of tap water

Broccoli seeds can be germinated by leaving them in dark conditions under tap water for 2 days, while irradiating the LED light source for 5 days while supplying tap water to obtain broccoli sprouts. At this time, the temperature is 22 to 25 ° C, and the humidity is 40 to 60%.

Example 1. Lava seawater_100 ppm

Broccoli seeds were left to germinate for 2 days in dark conditions under water containing 100 ppm of lava seawater, and then irradiated with LED light for 5 days while supplying tap water to obtain broccoli sprouts. At this time, the lava seawater was treated with an electrodialysis device for 5 minutes to use an electrical conductivity of 12 mS / cm. The temperature during germination and growth was 22 to 25 ° C and the humidity was 40 to 60%.

Example 2. Lava seawater_300 ppm

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 300 ppm.

Example 3. Lava seawater_500 ppm

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 500 ppm.

Example 4. Lava seawater_1000 ppm

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 1000 ppm.

Example 5. Lava seawater_3000 ppm

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 3000 ppm.

Example 6. Lava seawater_5000 ppm

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 5000 ppm.

Example 7. Omit the process of treating lava seawater with an electrodialysis device

The same procedure as in Example 1 was carried out, but lava seawater was used without treatment with an electrodialysis apparatus to obtain broccoli sprouts.

<Test Example_1>

Test Example 1. Growth rate measurement

The growth rate was obtained by measuring the length of the broccoli sprouts obtained according to the control group and Examples 1 to 7.

division Control Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Growth rate
(cm / day) 0.62 1.26 1.30 1.50 1.51 1.31 1.17 0.90

As shown in Table 1 above, it was confirmed that the broccoli sprouts produced according to Examples 1 to 7 of the present invention showed superior growth rates compared to the control group.

In particular, it was confirmed that the broccoli sprouts of Examples 3 and 4 showed significantly better growth rates than the other groups.

Test Example 2. Measurement of sulforaphane content

The content of sulfolapan contained in broccoli sprouts prepared according to Examples 1 to 7 was measured according to the conditions in Table 2 below.

division Control Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Sulforaphane content
(ppm) 781 1,510 2,668 5,116 5,890 2,488 1,438 1,302

As shown in Table 3 above, it was confirmed that the broccoli sprouts produced according to Examples 1 to 7 of the present invention had significantly higher sulforaphane content than the control group.

In particular, it was confirmed that the broccoli sprouts of Examples 3 and 4 had a sulforaphane content of 2 times higher than that of the other groups.

In the following, a test was performed using Example 4, which has the best growth rate and sulforaphane content.

[ PEF  Treatment and other water use]

Example  4. Lava seawater

Although carried out in the same manner as in Example 1, broccoli sprouts were obtained using lava seawater at a concentration of 1000 ppm.

Example  8. Lava seawater  + PEF

1000 mL of lava seawater was added to a batch processing vessel (300 ml) in which electrodes were disposed at intervals of 80 mm with a 5 kW-class pulse generator, and after the broccoli sprouts prepared according to Example 4 were introduced, an out voltage of 70% Broccoli sprouts were obtained by treatment with a high voltage pulsed electric field (PEF) for 3 seconds at (out voltage), pulse width of 25 us, frequency of 110 Hz and energy of 7 kJ / kg.

Comparative example  1. General seawater

Although carried out in the same manner as in Example 8, broccoli sprouts were obtained using normal seawater instead of lava seawater.

Comparative example  2. General seawater + PEF

Although carried out in the same manner as in Example 8, broccoli sprouts were obtained using normal seawater instead of lava seawater, and broccoli sprouts were obtained by treating a high voltage pulse electric field (PEF) under normal seawater.

Comparative example  3. Deep ocean water

Although carried out in the same manner as in Example 8, broccoli sprouts were obtained using deep ocean water instead of lava seawater.

Comparative example  4. Deep ocean water  + PEF

Although carried out in the same manner as in Example 8, broccoli sprouts were obtained using deep ocean water instead of lava seawater, and broccoli sprouts were obtained by treating a high voltage pulse electric field (PEF) under deep ocean water.

Comparative example  5. Tap water + PEF

1000 mL of tap water was added to a batch processing vessel (300 ml) in which electrodes were disposed at intervals of 80 mm with a 5 kW-class pulse generator, and broccoli sprouts prepared according to the control were added, followed by an out voltage of 70%. ), A high voltage pulsed electric field (PEF) for 3 seconds with a pulse width of 25 μs, a frequency of 110 Hz and energy of 7 kJ to obtain broccoli sprouts.

[Table 4] below shows the mineral concentrations of lava seawater before electrodialysis, general seawater, and deep ocean water.

Category (unit: ppm) Lava seawater General seawater Deep ocean water Na 10510 10762 11000 Mg 1295 1294 1270 Ca 399 413 474 K 400 387 403 Br 63.4 67 80.3 Sr 7.70 7.8 8.03 Zn 0.019 0.00035 <0.005 Fe 0.015 0.00003 <0.03 Mn 0.008 0.00002 <0.005 V 0.015 0.002 - Se 0.013 0.001 <0.005 Ge 0.002 - -

As shown in Table 4 above, it was confirmed that lava seawater, general seawater, and deep ocean water are different waters having different mineral concentrations.

In particular, the lava seawater was found to have significantly higher contents of zinc (Zn), iron (Fe), manganese (Mn), vanadium (V), selenium (Se), and germanium (Ge) than normal seawater and deep ocean water. These ingredients seem to influence the growth rate of broccoli sprouts and the improvement of sulforaphane content.

< Test example _2>

Test example  3. Measurement of growth rate

The same method as in Test Example 1 was measured. Since Example 8, Comparative Example 2, Comparative Example 4 and Comparative Example 5 were treated with PEF on the grown broccoli sprout, the growth rate was meaningless and was omitted.

division Example 4 Comparative Example 1 Comparative Example 3 Growth rate
(cm / day) 1.51 0.84 0.79

As shown in Table 5 above, it was confirmed that the broccoli sprouts produced according to Example 4 of the present invention showed significantly superior growth rates compared to Comparative Examples 1 and 3.

The broccoli sprouts of Comparative Examples 1 and 3 were not significantly different from the growth rate of the control group.

Test Example 4. Measurement of sulfolapan content

The same method as in Test Example 2 was measured.

division Example 4 Example 8 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Sulforaphane content
(ppm) 5,890 16,864 1,164 2,048 881 1,560 1,194

As shown in Table 6 above, it was confirmed that the broccoli sprouts produced according to Examples 4 and 8 of the present invention had a significantly higher sulforaphane content compared to Comparative Examples 1 to 5.

In particular, it was confirmed that the broccoli sprouts of Example 8 had a sulforaphane content of about 3 times higher than that of Example 4 broccoli sprouts.

Test example  5. Measurement of some mineral content

The useful mineral content of broccoli sprouts produced according to Example 4 and Comparative Examples 1 and 3 was measured using ICP-MS.

division Example 4 Comparative Example 1 Comparative Example 3 Zn 0.008 0.000021 - Fe 0.005 - - Mn 0.003 - - V 0.007 0.001 - Se 0.006 0.0005 - Ge 0.001 - -

As shown in Table 7 above, the broccoli sprouts prepared according to Example 4 compared to Comparative Examples 1 and 3 were zinc (Zn), iron (Fe), manganese (Mn), vanadium (V), selenium (Se), It was confirmed that the content of germanium (Ge) was significantly higher.

Claims (8)

(A) germinating broccoli seeds under water containing electrodialyzed lava seawater;
(B) supplying the water containing the lava seawater to the germinated broccoli seeds; includes,
The concentration of the lava seawater is 300 to 3000 ppm, and the electrical conductivity of the lava seawater is 5 to 30 mS / cm. The method for producing broccoli sprouts with increased growth rate and sulforaphane content. According to claim 1, After the step (B) (C) after the broccoli sprouts grown with water containing the lava seawater is introduced into a batch or continuous processing vessel, the out voltage (out voltage) 50 to 80%, pulse The growth rate and sulforaphane content of broccoli sprouts increased by 15 to 35 μs in width, 50 to 200 Hz in frequency, and 1 to 10 kJ in energy for 1 to 10 seconds. Production method. delete delete The method of claim 1, wherein the lava seawater is 0.01 to 0.03 ppm of zinc, 0.01 to 0.03 ppm of iron, 0.006 to 0.02 ppm of manganese, 0.01 to 0.03 ppm of vanadium, 0.01 to 0.03 ppm of selenium, and 0.001 to 0.005 ppm of germanium. Method of producing broccoli sprouts with increased growth rate and sulforaphane content, characterized by being included. [3] The method of claim 2, wherein the batch-type or continuous-processing vessel is provided with a plurality of electrodes at intervals of 50 to 200 mm. Germinate and grow broccoli seeds with water containing electrodialyzed lava seawater,
The concentration of the lava seawater is 300 to 3000 ppm, and the electrical conductivity of the lava seawater is a broccoli sprout with increased growth rate and sulforaphane content, characterized in that 5 to 30 mS / cm. The method of claim 7, wherein after the broccoli sprouts grown with water containing lava seawater are introduced into a batch or continuous processing vessel, an out voltage of 50 to 80%, a pulse width of 15 to 35 μs, and a frequency of 50 to 200 Broccoli sprouts with increased growth rate and sulforaphane content, characterized by treatment with a high voltage pulsed electric field for 1 to 10 seconds to give a total energy of 1 to 30 kJ in Hz.