KR102062790B1 - Collagen of dried yellow pollack and manufacturing method thereof - Google Patents
Collagen of dried yellow pollack and manufacturing method thereof Download PDFInfo
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- KR102062790B1 KR102062790B1 KR1020180058680A KR20180058680A KR102062790B1 KR 102062790 B1 KR102062790 B1 KR 102062790B1 KR 1020180058680 A KR1020180058680 A KR 1020180058680A KR 20180058680 A KR20180058680 A KR 20180058680A KR 102062790 B1 KR102062790 B1 KR 102062790B1
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 28
- 108010035532 Collagen Proteins 0.000 title claims abstract description 28
- 229920001436 collagen Polymers 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 241001098054 Pollachius pollachius Species 0.000 title 1
- 239000006227 byproduct Substances 0.000 claims abstract description 30
- 239000002244 precipitate Substances 0.000 claims abstract description 18
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052717 sulfur Inorganic materials 0.000 claims abstract description 14
- 239000011593 sulfur Substances 0.000 claims abstract description 14
- 239000008188 pellet Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 10
- 239000008213 purified water Substances 0.000 claims abstract description 9
- 230000002797 proteolythic effect Effects 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 5
- 230000007935 neutral effect Effects 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 239000004382 Amylase Substances 0.000 claims description 4
- 102000013142 Amylases Human genes 0.000 claims description 4
- 108010065511 Amylases Proteins 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 235000019418 amylase Nutrition 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 230000037319 collagen production Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 230000018044 dehydration Effects 0.000 abstract description 2
- 238000006297 dehydration reaction Methods 0.000 abstract description 2
- 239000003245 coal Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 2
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 235000001258 Cinchona calisaya Nutrition 0.000 description 1
- 241001660788 Cinchona calisaya Species 0.000 description 1
- 241001313700 Gadus chalcogrammus Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 102000005158 Subtilisins Human genes 0.000 description 1
- 241000610628 Trichoptilium incisum Species 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002220 antihypertensive agent Substances 0.000 description 1
- 229940127088 antihypertensive drug Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- -1 flavorzyme Proteins 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/342—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of collagen; of gelatin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/10—Fish meal or powder; Granules, agglomerates or flakes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/06—Function of food ingredients pH modification agent
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/34—Membrane process
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 황태 콜라겐 제조방법에 관한 것으로, (a) 황태 부산물 분말을 이용하여 황태 부산물 펠렛을 제조하는 단계와; (b) 정제수 100 중량부에 대하여, 상기 황토 부산물 펠렛 20 ~ 25 중량부를 충전한 후 원심 분리하여 상등액을 제거하고 침전물을 탈수하는 단계와; (c) 탈수된 침전물을 0.3 ~ 1.5N HCl로 적정한 다음 10 ~ 14시간 교반한 후 상기 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 첨가하여 5 ~ 10일간 정치하는 단계와; (d) 산도 조절제를 첨가하여 중성 용액을 만든 후, 원심 분리하여 상등액은 제거하는 단계와; (e) 상기 (d)단계의 침전물을 여과, 탈수 건조하여 분말 성상의 황태 콜라겐을 최종 제조하는 단계로 구성됨으로써, 황태 부산물을 이용하여 황태 콜라겐을 대량 제조할 수 있는 효과가 있다.The present invention relates to a method for producing yellow collagen, the method comprising the steps of: (a) preparing a yellow coal by-product pellet using yellow coal by-product powder; (b) per 100 parts by weight of purified water, followed by centrifugation to remove 20 to 25 parts by weight of the ocher by-product pellets to remove the supernatant and dehydrate the precipitates; (c) titrating the dehydrated precipitate with 0.3-1.5N HCl, stirring for 10-14 hours, and then adding 1-5 parts by weight of the proteolytic agent to 100 parts by weight of the dehydrated precipitate, and then leaving the mixture for 5 to 10 days; (d) adding a pH regulator to make a neutral solution, and then centrifuging to remove the supernatant; (e) By filtration, dehydration drying the precipitate of step (d) is composed of the step of final production of the powdery yellow collagen, there is an effect that can be produced a large amount of yellow collagen using the by-products of sulfur.
Description
본 발명은 황태 콜라겐 및 이의 제조방법에 관한 것이다.The present invention relates to yellow collagen and a method for producing the same.
어류 콜라겐은 3천개 이상의 아미노산이 뭉쳐진 동물성 고분자 콜라겐과는 달리 저분자 콜라겐으로 체내 흡수율이 84%에 이르는 고급 콜라겐이다. 이러한 어류 콜라겐 중 황태 콜라겐은 황태 가공시 발생되는 부산물을 이용하여 제조 단가는 낮으나 대량 생산이 불가능한 단점이 있었다.Fish collagen, unlike animal polymer collagen, which contains more than 3,000 amino acids, is a high-molecular collagen with 84% absorption. Among these fish collagen, yellow collagen has a disadvantage in that the manufacturing cost is low but mass production is impossible using the by-product generated during the processing of the yellow.
이러한 단점을 해소하기 위하여 창안된 대한민국 등록특허 제0946997호(등록일자: 2010.3.4., 발명의 명칭: 황태 가수분해물, 2차 가수분해물 또는 이로부터 분리한 활성 펩티드를 유효성분으로 함유하는 조성물)는 1) 황태(Theragra chalcogramma) 분쇄한 후 증류수를 혼합하여 믹서를 통해 고압균질화된 혼합물을 수득하는 단계; 2) 상기 단계 1)의 혼합물을 원심분리한 후 분리된 3개의 층 중에서 중간층을 수득하는 단계; 3) 상기 단계 2)의 중간층을 동결건조하여 가용성 단백질을 수득하는 단계; 4) 상기 단계 3)의 가용성 단백질에 펩신을 처리하여 1차 가수분해물을 수득하는 단계; 및 5) 상기 단계 4)의 1차 가수분해물을 추가로 알칼라제(alcalase), 플라보르자임(Flavourzyme), 프로나제 E(pronase E) 및 트립신(trypsin)으로 이루어진 군으로부터 선택된 어느 하나의 효소를 처리하여 2차 가수분해물을 수득하는 단계를 포함하는 방법에 의해 제조된, 황태의 가용성 단백질의 2차 가수분해물을 유효성분으로 함유하는 고혈압 예방 및 치료제가 개시되어 있다.Republic of Korea Patent No. 0946997 (Registration Date: 2010.3.4., Name of the invention: sulfur hydrolyzate, secondary hydrolyzate or composition containing the active peptide separated therefrom as an active ingredient) 1) the step (Theragra chalcogramma) pulverized and mixed with distilled water to obtain a high pressure homogenized mixture through a mixer; 2) centrifuging the mixture of step 1) to obtain an intermediate layer from the three separated layers; 3) lyophilizing the intermediate layer of step 2) to obtain a soluble protein; 4) plycine the soluble protein of step 3) to obtain a primary hydrolysate; And 5) any one enzyme selected from the group consisting of alcalase, flavorzyme, pronase E and trypsin, further comprising the first hydrolyzate of step 4). Disclosed is a hypertension prevention and treatment agent containing a secondary hydrolyzate of soluble protein of the sulfur produced by the method comprising the step of obtaining a secondary hydrolyzate as an active ingredient.
이러한 종래 기술은, 황태의 가용성 단백질에 효소를 처리하여 얻은 가수분해물, 2차 가수분해물 또는 이로부터 분리한 활성 펩티드는 과산화지질 형성 억제 활성, ACE(angiotensin-converting enzyme) 저해 활성 또는 프로테아제(protease) 활성을 나타냄으로써 항산화제 또는 고혈압 치료제에 유용하게 이용될 수 있다고 주장하는 반면, 제조 공정이 복잡하여 대량생산이 용이하지 못한 근원적인 문제점이 있었다.This prior art, hydrolysates, secondary hydrolysates or active peptides obtained by treating enzymes with soluble proteins in the yellow are characterized in that the lipid peroxidation inhibitory activity, angiotensin-converting enzyme (ACE) inhibitory activity or protease Insisting that the activity can be useful for antioxidants or antihypertensive drugs, while the manufacturing process is complex, there is a fundamental problem that mass production is not easy.
상기와 같은 문제점을 해결하고자 창출된 본 발명의 목적은, 황태 부산물을 활용하여 콜라겐을 대량 생산할 수 있는 황태 콜라겐 제조방법 및 이러한 방법에 의해 제조된 황토 콜라겐을 제공하는 데 있다.An object of the present invention created to solve the above problems is to provide a method for producing yellow collagen, which can mass-produce collagen by using yellow by-products, and to provide ocher collagen produced by such a method.
이러한 본 발명의 목적은, (a) 황태 부산물 분말을 이용하여 황태 부산물 펠렛을 제조하는 단계와; (b) 정제수 100 중량부에 대하여, 상기 황토 부산물 펠렛 20 ~ 25 중량부를 충전한 후 원심 분리 후 여과를 통해 상등액을 제거하고 침전물을 탈수하는 단계와; (c) 탈수된 침전물을 0.3 ~ 1.5N HCl로 적정한 다음 10 ~ 14시간 교반한 후 상기 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 첨가하여 5 ~ 10일간 정치하는 단계와; (d) 산도 조절제를 첨가하여 중성 용액을 만든 후, 원심 분리하여 상등액은 제거하는 단계와; (e) 상기 (d)단계의 침전물을 여과, 탈수 건조하여 분말 성상의 황태 콜라겐을 최종 제조하는 단계로 이루어진 황태 콜라겐 제조방법에 의해 달성될 수 있다.The object of the present invention, (a) using the yellow by-product powder to prepare a yellow by-product pellet; (b) filling the 20 to 25 parts by weight of the ocher byproduct pellets with respect to 100 parts by weight of purified water, followed by centrifugation to remove the supernatant by filtration and dehydrating the precipitates; (c) titrating the dehydrated precipitate with 0.3-1.5N HCl, stirring for 10-14 hours, and then adding 1-5 parts by weight of the proteolytic agent to 100 parts by weight of the dehydrated precipitate, and then leaving the mixture for 5 to 10 days; (d) adding a pH regulator to make a neutral solution, and then centrifuging to remove the supernatant; (e) The precipitate of step (d) may be achieved by filtration, dehydration drying, and the preparation of yellow collagen, which comprises the final preparation of powdery yellow collagen.
바람직하게는, 본 발명의 상기 (a)단계는, (가) 황태 부산물을 분쇄기를 이용하여 분쇄하여 황태 부산물 분말을 제조하는 단계와; (나) 황태 부산물 분말 100 중량부에 대하여, 정제수 30 ~ 35 중량부 및 전분 3 ~ 5 중량부를 혼합하여 교반하여 황태 반죽물을 만드는 단계와; (다) 상기 황태 반죽물을 압출기에 충전하여 직경 5 ~ 15㎜ 길이 10 ~ 20㎜로 절단한 후 제습 건조하여 황태 부산물 펠렛을 완성하는 단계로 이루어지는 것을 특징으로 한다.Preferably, the step (a) of the present invention comprises the steps of: (A) pulverizing the by-products of the sulfur by using a mill to produce a powder of the by-products; (B) mixing 100 to 5 parts by weight of the yellow powder by-product, and mixing 30 to 35 parts by weight of purified water and 3 to 5 parts by weight of starch to make a yellow dough; (C) characterized in that it comprises the step of filling the dough dough in the extruder to cut into diameter 5 ~ 15㎜ 10 ~ 20㎜ in length and then dehumidifying and drying the finished by-product pellets.
바람직하게는, 본 발명의 상기 (b)단계는 정제수 100 중량부에 대하여 아밀라아제 0.1 ~ 0.3 중량부를 더 첨가하고, 상기 (c)단계는 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 더 첨가하는 것을 특징으로 한다.Preferably, step (b) of the present invention further adds 0.1 to 0.3 parts by weight of amylase, based on 100 parts by weight of purified water, and step (c) is based on 1 to 5 parts by weight of protein degrading agent based on 100 parts by weight of the dehydrated precipitate. It is characterized by further adding part.
바람직하게는, 본 발명의 상기 (e)단계 이후에, 분말 성상의 황태 콜라겐을 멸균 증류수 또는 완충 용액을 첨가하여 현탁액을 만든 후 2 ~ 5℃에서 10 ~ 15일간 숙성하여 겔 상태의 콜라겐을 제조하는 (f)단계를 더 포함하는 것을 특징으로 한다.Preferably, after step (e) of the present invention, the powdery yellow collagen is added to sterile distilled water or a buffer solution to make a suspension, and then aged at 2 to 5 ° C. for 10 to 15 days to prepare gel collagen. It characterized in that it further comprises the step (f).
또한, 본 발명의 다른 목적은, 위 방법들 중 어느 하나에 의해 생산된 황토 콜라겐에 의해 달성될 수 있다.In addition, another object of the present invention can be achieved by ocher collagen produced by any of the above methods.
이상과 같은 본 발명은 황태 부산물을 이용하여 황태 콜라겐을 대량 제조할 수 있는 효과가 있다.The present invention as described above has the effect of mass production of yellow collagen using the by-products of yellow.
이하, 본 발명의 실시예를 참조하여 설명하기로 한다.Hereinafter, with reference to the embodiment of the present invention will be described.
1. 황태 부산물 펠렛 만들기1. Making the By-product Pellets
본 실시예의 황태 부산물은 황태 머리, 황태 꼬리, 황태 껍질, 황태 뼈 및 황태 가공시 발생되는 황태 분말을 의미한다. 그리고 본 실시예에서 황태 부산물 펠렛을 만드는 이유는 황태 콜라겐의 원료를 펠렛으로 규격화하여 보관이나 정량 투입이 용이하며 추후 진행되는 반응 속도를 증가시키기 위한 것이다.By-products of the present embodiment means yellow head, yellow tail, yellow bark, yellow bone, and yellow powder generated during processing. And the reason for making the sulfur by-product pellets in the present embodiment is to standardize the raw material of the collagen into pellets for easy storage or quantitative input, and to increase the reaction rate to be carried out later.
(가) 황태 부산물을 분쇄기를 이용하여 분쇄하여 황태 부산물 분말을 제조한다.(A) Sulfur by-products are pulverized using a grinder to prepare sulfur by-product powders.
(나) 황태 부산물 분말 100 중량부에 대하여, 정제수 30 ~ 35 중량부 및 전분 3 ~ 5 중량부를 혼합하여 교반하여 황태 반죽물을 만든다.(B) With respect to 100 parts by weight of sulfur by-product powder, 30 to 35 parts by weight of purified water and 3 to 5 parts by weight of starch are mixed and stirred to make a yellow dough.
(다) 황태 반죽물을 압출기에 충전하여 직경 5 ~ 15㎜ 길이 10 ~ 20㎜로 절단한 후 제습 건조하여 황태 부산물 펠렛을 완성한다. 여기서, 압출기의 온도는 120 ~ 145℃이다.(C) Fill the yellow dough with an extruder, cut into diameters of 5 to 15 mm and 10 to 20 mm in length, then dehumidify and dry to complete the by-product pellets. Here, the temperature of an extruder is 120-145 degreeC.
2. 정제수 100 중량부에 대하여, 황토 부산물 펠렛 20 ~ 25 중량부 및 아밀라아제 0.1 ~ 0.3 중량부를 충전한 후 원심 분리하여 상등액을 제거하고 침전물을 탈수한다. 여기서, 상등액에는 황태 부산물을 펠렛화할 때 사용된 전분은 아밀라아제에 의해 용해되어 있다.2. To 100 parts by weight of purified water, 20 to 25 parts by weight of ocher by-product pellets and 0.1 to 0.3 parts by weight of amylase were charged, followed by centrifugation to remove the supernatant and dewatering the precipitate. Here, the starch used when pelleting the sulfur by-products is dissolved in amylase in the supernatant.
3. 탈수된 침전물을 0.3 ~ 1.5N HCl로 적정한 다음 10 ~ 14시간 교반한 후 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 첨가하여 5 ~ 10일간 정치한다.3. The dehydrated precipitate is titrated with 0.3 ~ 1.5N HCl and stirred for 10 to 14 hours, and then 1 to 5 parts by weight of the proteolytic agent is added to 100 parts by weight of the dehydrated precipitate and left to stand for 5 to 10 days.
여기서, 단백질 분해제는 단백질 분해효소 또는 단백질 가수분해제이며, 단백질 분해효소는 펩신이고, 단백질 가수분해제는 매우 다양하며 이는 공지기술로서 이에 대한 상세한 설명은 생략하기로 한다.Here, the proteolytic agent is a protease or a proteolytic agent, the protease is pepsin, and the proteolytic agent is very diverse, which will be omitted as a detailed description thereof.
4. 산도 조절제를 첨가하여 중성 용액을 만든 후, 원심 분리하여 상등액은 제거한다. 여기서, 산도 조절제는 NaCl이며, 제거된 상등액에는 가수분해된 단백질이 존재한다.4. Add acidity regulator to make neutral solution, then centrifuge to remove supernatant. Here, the acidity regulator is NaCl, and there is a hydrolyzed protein in the supernatant removed.
5. 원심 분리하여 상등액을 제거하고 침전물을 여과, 탈수 건조하여 분말 성상의 황태 콜라겐을 최종 제조한다.5. The supernatant is removed by centrifugation, and the precipitate is filtrated and dehydrated and dried to finally prepare powdery yellow collagen.
여기서, 황태 콜라겐을 멸균 증류수 또는 완충 용액을 첨가하여 현탁액을 만든 후 2 ~ 5℃에서 10 ~ 15일간 숙성하여 겔 상태의 콜라겐을 제조할 수 있다.Here, yellow collagen may be prepared by adding sterile distilled water or a buffer solution to prepare a suspension, and then aging at 2 to 5 ° C. for 10 to 15 days to prepare gel collagen.
이상과 같은 본 발명은 일 실시예에 한정되어 설명되었으나, 이에 한정되지 않고 본 발명의 기술적 사상을 토대로 변형되는 실시예들은 모두 본 발명의 권리범위에 속함이 분명하다.The present invention as described above has been described with reference to one embodiment, but not limited to this embodiment is obvious that all modifications based on the technical spirit of the present invention belong to the scope of the present invention.
Claims (5)
(b) 정제수 100 중량부에 대하여, 상기 황태 부산물 펠렛 20 ~ 25 중량부를 충전한 후 원심 분리 후 여과를 통해 상등액을 제거하고 침전물을 탈수하는 단계와;
(c) 탈수된 침전물을 0.3 ~ 1.5N HCl로 적정한 다음 10 ~ 14시간 교반한 후 상기 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 첨가하여 5 ~ 10일간 정치하는 단계와;
(d) 산도 조절제를 첨가하여 중성 용액을 만든 후, 원심 분리하여 상등액은 제거하는 단계와;
(e) 상기 (d)단계의 침전물을 여과, 탈수 건조하여 분말 성상의 황태 콜라겐을 최종 제조하는 단계로 이루어지되,
상기 (a)단계의 황태 부산물 펠렛은 (가) 황태 부산물을 분쇄기를 이용하여 분쇄하여 황태 부산물 분말을 제조하는 단계와; (나) 황태 부산물 분말 100 중량부에 대하여, 정제수 30 ~ 35 중량부 및 전분 3 ~ 5 중량부를 혼합하여 교반하여 황태 반죽물을 만드는 단계와; (다) 상기 황태 반죽물을 압출기에 충전하여 직경 5 ~ 15㎜ 길이 10 ~ 20㎜로 절단한 후 제습 건조하는 단계로 제조되고,
상기 (b)단계는 정제수 100 중량부에 대하여 아밀라아제 0.1 ~ 0.3 중량부를 더 첨가하고, 상기 (c)단계는 탈수된 침전물 100 중량부에 대하여 단백질 분해제 1 ~ 5 중량부를 더 첨가하고,
상기 (e)단계 이후에, 분말 성상의 황태 콜라겐을 멸균 증류수 또는 완충 용액을 첨가하여 현탁액을 만든 후 2 ~ 5℃에서 10 ~ 15일간 숙성하여 겔 상태의 콜라겐을 제조하는 (f)단계를 더 포함하는 것을 특징으로 하는 황태 콜라겐 제조방법.
(a) preparing a byproduct pellet by using a byproduct powder;
(b) charging the sulfur byproduct pellet to 20 to 25 parts by weight based on 100 parts by weight of purified water, followed by centrifugation to remove the supernatant by filtration and dehydrating the precipitate;
(c) titrating the dehydrated precipitate with 0.3-1.5N HCl, stirring for 10-14 hours, and then adding 1-5 parts by weight of the proteolytic agent to 100 parts by weight of the dehydrated precipitate, and then leaving the mixture for 5 to 10 days;
(d) adding a pH regulator to make a neutral solution, and then centrifuging to remove the supernatant;
(e) filtering the precipitate of step (d), and dehydrating and drying to form the final collagen powdery yellow collagen,
The sulfur by-product pellet of step (a) comprises the steps of: (A) pulverizing the sulfur by-product using a grinder to produce a sulfur by-product powder; (B) mixing 100 to 5 parts by weight of the by-product powder of sulfur, mixing 30 to 35 parts by weight of purified water and 3 to 5 parts by weight of starch to make a yellow dough; (C) is prepared by the step of filling the dough dough in the extruder and cut into diameter 5 ~ 15㎜ length 10 ~ 20㎜ and then dehumidifying and drying,
Step (b) further adds 0.1 to 0.3 parts by weight of amylase based on 100 parts by weight of purified water, and step (c) further adds 1 to 5 parts by weight of a proteolytic agent to 100 parts by weight of dehydrated precipitates,
After step (e), the powdery yellow collagen is added to sterile distilled water or a buffer solution to make a suspension, and then aged at 2 to 5 ° C for 10 to 15 days to prepare gel collagen in step (f). Yellow collagen production method comprising a.
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