KR102054861B1 - Aquaculture method using a reducing stress and enhancing immunity by irradiation of light emitting diode in the shrimp - Google Patents

Abstract

The present invention relates to a method for farming shrimp using stress reduction and immune system enhancement by irradiation of light emitting diode light source, and more specifically, stress by irradiating LED light source of specific wavelength to shrimp exposed to the environment of rapid salinity change and water temperature change. It relates to a method of farming to be able to suppress and enhance immunity.
The present invention, the first step of preparing a shrimp; A second step of stocking shrimps in a water bath containing salt water; By irradiating the light source of any one or more of red LED or green LED under the condition of changing the salinity and temperature of water to suppress the oxidative stress of shrimps to enhance the immunity; by a light emitting diode light source comprising a Shrimp farming methods using stress reduction and immunity enhancement are technical points.

Description

Aquaculture method using a reducing stress and enhancing immunity by irradiation of light emitting diode in the shrimp}

The present invention relates to a method for farming shrimp using stress reduction and immune system enhancement by irradiation of light emitting diode light source, and more specifically, stress by irradiating LED light source of specific wavelength to shrimp exposed to the environment of rapid salinity change and water temperature change. It relates to a method of farming to be able to suppress and enhance immunity.

In general, among the environmental factors that cause stress in aquatic life, light is an important environmental factor that regulates biorhythms and endocrine signals, and directly affects growth, maturation, immunity and stress response.

Fish are classified as swimming organisms that can choose their habitat. However, shrimps are less mobile than fish, so they are directly exposed to environmental changes and pollution.

In particular, since shrimps are often reared for indoor ornamental use, a large amount of death occurs due to frequent exposure to stress factors derived from a closed environment.

For example, cleaner shrimps, which are widely known as expensive ornamental shrimps around the world, including Korea, are generally characterized by ingesting (cleaning) parasites in and out of fish while maintaining a symbiotic relationship with fish. It occupies a very important position in the ornamental biological industry.

However, shrimps, such as cleaner shrimp, cannot solve the stressors caused by the closed environment, so mass production technology for shrimps has not been developed yet. For this reason, it is time to study the (physical) physiological aspects such as light reaction and environmental changes using shrimp.

Korean Patent Publication No. 10-2016-0149760, published on December 28, 2016. Registered in Korea Registration No. 20-0401512, registered on November 10, 2005. Registered Korean Patent Publication No. 10-1800810, filed on November 17, 2017.

The present invention has been invented to solve the above problems, the light emitting diode that can suppress the stress and enhance the immunity by irradiating the LED light source of a specific wavelength to shrimp exposed to the environment of rapid salinity change and water temperature change The purpose of the present invention is to provide a farming method using stress reduction and immune system enhancement by shrimp irradiation.

The present invention for achieving the above object, to reduce the mortality of shrimp exposed to various environments, the first step of preparing a shrimp; A second step of stocking the shrimps in a water tank containing salt water; And the shrimp is exposed to a condition in which the salinity of the water is any one of 25 psu, 30 psu, 35 psu, and 40 psu, or the temperature of the water is 26 ° C. for 2 weeks, followed by any one of 21 ° C., 26 ° C., and 31 ° C. When exposed to a red LED light source of 600 ~ 650nm wavelength, the third step of reducing the oxidative stress of shrimps to increase the immunity; In the third step, Brain, gill ( By receiving light from Eyetalk, which has higher melatonin activity than Gill, Digestive duct and Hepatopancreas, it reduces oxidative stress of shrimp caused by reactive oxygen species, Melatonin The method of the present invention focuses on a method of reducing the stress of shrimps by a light emitting diode light source and enhancing the immunity to increase the immunity due to increased activity.

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Aquaculture method using stress reduction and immunity enhancement of shrimps by the light emitting diode light source irradiation according to the present invention by the solution of the above problem, red LED having a specific wavelength to shrimps under conditions exposed to rapid salinity changes and water temperature changes environment By irradiating the light source, not only can reduce the salt stress of shrimp, but also has the effect of enhancing the immunity.

1 is a graph of the LED light source wavelength according to a preferred embodiment of the present invention.
Figure 2 is a light cycle and optical rhythm graph of the brain (Brain) according to a preferred embodiment of the present invention.
3 is a light cycle and optical rhythm graph of the eye cycle (Eyestalk) according to a preferred embodiment of the present invention.
Figure 4 is a graph of the light cycle and biorhythm of the gill (Gill) according to a preferred embodiment of the present invention.
FIG. 5 is a graph illustrating light response and biorhythm of light cycles in a digestive duct according to a preferred embodiment of the present invention. FIG.
Figure 6 is a light cycle and biorhythm graph of the light cycle of the hepatopancreas (Hepatopancreas) according to a preferred embodiment of the present invention.
Figure 7 is a light response and biorhythm graph for each light source of the brain (Brain) according to a preferred embodiment of the present invention.
8 is a light response and biorhythm graph for each light source of Eyestalk according to a preferred embodiment of the present invention.
9 is a light response and biorhythm graph of each gill (Gill) according to a preferred embodiment of the present invention.
10 is a light response and biorhythm graph of light source of hepatopancreas according to a preferred embodiment of the present invention.
11 is a light response and biorhythm graph for each light source of the digestive duct according to a preferred embodiment of the present invention.
FIG. 12 is a graph showing light response and clock gene (Cry1) expression biorhythms of light sources according to a preferred embodiment of the present invention.
FIG. 13 is a graph of light response and clock gene (Cry1) expression rhythm of light source of eye disease according to a preferred embodiment of the present invention.
14 is a graph of light response and clock gene (Cry1) expression biorhythm of hepatopancreas according to a preferred embodiment of the present invention.
FIG. 15 is a graph illustrating light response and time gene expression (Per2) according to a light source of the brain according to a preferred embodiment of the present invention.
16 is a graph of light response and visual gene (Per2) expression rhythm for each light source of Eyestalk according to a preferred embodiment of the present invention.
FIG. 17 is a graph showing light response and clock gene (Per2) expression biorhythm of hepatopancreas according to a preferred embodiment of the present invention. FIG.
18 is a tissue-specific optical response and salt stress analysis (NKA) graph according to a preferred embodiment of the present invention.
19 is a tissue-specific optical response and salt stress analysis (SOD) graph according to a preferred embodiment of the present invention.
20 is a tissue-specific optical response and salt stress analysis (CAT) graph according to a preferred embodiment of the present invention.
21 is an activity indicator of the cleaner shrimp by the water temperature change in accordance with a preferred embodiment of the present invention.

I. Instrument of the invention

Prior to describing the present invention, since shrimps are in the spotlight as high-value coronary organisms, the technique for producing a large amount of aquaculture is still under development, and thus, a point of need for a study of (reproduction) physiological aspects has been reached.

For this reason, not only maturation but also stress-related studies are required for stable production of shrimps. Especially, shrimps are sensitive to light, and therefore, various LED wavelengths and photoreaction mechanisms need to be studied.

In other words, the reason why the research required when shrimp were exposed to various environmental changes ^{_{Superoxide (O 2 -), Hydrogen}} peroxide (H 2 O 2), Hydroxyl radical radical oxidative stress and paper occurs, such as (HO) (Oxidative As stress occurs, disease resistance and reproduction ability decrease due to DNA destruction, lipid (over) oxidation, and oxidation of amino acids in proteins.

Accordingly, by understanding the light reaction mechanism of shrimps, the optimal light wavelength range is selected, the stress is controlled by the selected specific wavelength and the maturation is induced. Finally, the eco-friendly aquaculture environment using the specific light wavelength of the LED is created. It is intended to promote stable production technology by inducing the maturation and spawning of shrimps.

However, in the present invention, the experiment was conducted with a cleaner shrimp that is spotlighted as a tubular organism.

Ⅱ. Summary of the invention

Hereinafter, the preferred embodiment of the present invention will be described in detail.

Aquaculture method using the stress reduction and immune system of the shrimp by the light emitting diode light source irradiation of the present invention, the first step (S10) to prepare the shrimp, the second step of stocking the shrimp in the water tank containing the salt (S20) ), By irradiating a light source of any one or more of red LED or green LED under the condition of changing the salinity and temperature of water, it can be carried out in the third step (S30) to suppress the oxidative stress of shrimps to enhance immunity, in particular, the present invention In this paper, we will present a detailed description of the third stage.

As such, the method of confirming the inhibition of oxidative stress and the enhancement of immunity of shrimps according to the irradiation of the light source in the third step, first, the light response and biorhythm analysis of the shrimps, and second, (oxidation) according to the salinity and water temperature change factors by light wavelength There is an analysis of stress reduction effects.

First, photoreaction and biorhythm analysis of shrimps were performed by ① confirming the change of Melatonin concentration by light cycle to confirm the photoreaction characteristics, and ② ② confirming the expression of photoreaction genes and biorhythm-related clock genes by light wavelength. Refers to gene search and tissue expression analysis.

Second, analysis of (oxidation) stress reduction effect according to salinity change factors by light wavelength was performed by ① analysis of Na / K-ATPase activity change of shrimps by light wavelength and ② analysis of antioxidant enzyme changes of shrimp by light wavelength. Means an analysis of abatement effects.

Third, the analysis of stress reduction effect according to water temperature factors by light wavelength means the analysis of antioxidant enzymes, stress and immune marker activity in cleaner shrimps according to the water temperature change.

III. Method for Promoting Research with LED Light Sources of the Invention

(1) LED lighting production

1 is a graph showing an LED light source wavelength according to a preferred embodiment of the present invention. Referring to Figure 1, it shows a light source according to the irradiance (Watts / m ² ) for each wavelength band (nm), UV LED (350 ~ 410nm, more specifically 380nm), blue LED ( It can be confirmed that the experiment with 400 ~ 470nm, more specifically 460nm), green LED (500 ~ 550nm, more specifically 520nm), red LED (600 ~ 650nm, more specifically 630nm).

For reference, the reason why the LED is applied in the present invention is that the LED has low power consumption (half level of incandescent and fluorescent lamps) and long life (50,000 hours / 10 years or more), and does not use gas. This is because it is possible to make a light source that includes a large number of elements and emit a specific wavelength, and thus it is being spotlighted as a next generation light with low energy and high efficiency.

(2) Search and Cloning of Photoreactions and Clock Genes

Extraction of messenger RNA in brain, eye disease, gill, digestive duct and hepatopancreas by measuring changes in Melatonin concentration of cleaner shrimps The sequence is searched using the nucleotide sequence of other crustaceans.

(3) Exploration and Cloning of Stress and Antioxidant Related Genes

Antioxidant related gene cloning and molecular biological characteristics are investigated to explore and clone stress and antioxidant related genes.

(4) Analysis of stress reduction effect by salinity change by LED wavelength and intensity

Exposed shrimps to salinity changing environment (40psu, 35psu (general seawater), 30psu, 25psu) respectively to check the effect of stress reduction (modulation) by LED wavelength / intensity, and change of Na ⁺ / K ⁺ -ATPase in muscle and gill Check.

(5) Quantitative real-time PCR (QPCR)

The changes in the optical response and clock gene (Cry1, Per2) mRNA expression levels by LED wavelength and intensity are investigated.

(6) hormone analysis

The changes of antioxidant enzyme concentrations according to salinity change according to LED wavelength / intensity are investigated, and Melatonin concentration change of cleaner shrimp in the LED wavelength / intensity experiments is measured.

Ⅳ. Experimental setting of the present invention

(1) Photoreaction and biorhythm investigation of cleaner shrimp-light cycle

Light cycle

LD 12h Light: 12h Dark LL Continued light DD Continued dark

Breeding salt: 35 psu

Breeding period: 24 hours (2, 6, 10, 14, 18, 22h after light source irradiation)

Size and weight: 4.28 ± 0.23cm, 2.23 ± 0.15g

(2) Photo reaction and biorhythm investigation of cleaner shrimp-LED reaction

Light cycle: 12h Light: 12h Dark

Breeding salt: 35 psu

Breeding period: 24 hours (2, 6, 10, 14, 18, 22h after irradiation)

Size and weight: 4.28 ± 0.23cm, 2.23 ± 0.15g

Control zone Experiment: Fluorescent lamp (Cont.)

LED experiment

Experimental ZoneⅠ red wavelength (surface at 0.5 / 1.0 W / m ² ) Experimental Zone II green wavelength (surface level, 0.5 / 1.0W / m ² ) Experimental Section III blue wavelength (based on surface, 0.5 / 1.0 W / m ² ) Experimental IV UV wavelength (0.2 / 0.4 W / m ^{2 at} surface)

(3) Stress Reduction Effect by Salinity Factors by Light Wavelength

Light cycle: 12h Light: 12h Dark

Control salt: 35 psu (normal seawater)

Experimental salinity: 25, 30, 35, 40 psu

Size and weight: 4.42 ± 0.20cm, 2.41 ± 0.25g

LED experiment

Experiment Fluorescent lamp (Cont.) Experimental ZoneⅠ 25psu (70% SW) red wavelength (Based on surface, 0.5 / 1.0W / m ² ) Experimental Zone II 30psu (80% SW) green wavelength (Based on surface, 0.5 / 1.0W / m ² ) General seawater 35psu (100% SW) blue wavelength (Based on surface, 0.5 / 1.0W / m ² ) Experimental Section IV 40psu (115% SW) UV wavelength (Based on surface, 0.2 / 0.4W / m ² )

(4) Stress Reduction Effect According to Water Temperature Factors by Light Wavelength

Light cycle: 12h Light: 12h Dark

Control water temperature: 21 ℃ (general seawater)

Experimental water temperature: 21, 26, 31 ℃

Size and weight: 4.42 ± 0.20cm, 2.41 ± 0.25g

LED experiment

Control Cont. Experiment R 0.5 red LED (0.5W / m ² ) R 1.0 red LED (1.0W / m ² ) G 0.5 green LED (0.5W / m ² ) G 1.0 green LED (1.0W / m ² ) B 0.5 blue LED (0.5W / m ² ) B 1.0 blue LED (1.0W / m ² ) UV 0.2 UV LED (0.2W / m ² ) UV 0.4 UV LED (0.4W / m ² )

Ⅴ. Experiment of the present invention

(1) Light cycle-specific light response and biorhythm analysis (Melatonin)

FIG. 2 is a light cycle and light rhythm graph of a brain according to a preferred embodiment of the present invention, and FIG. 3 is a light cycle and light response of an eye bottle according to a preferred embodiment of the present invention. Figure 4 is a rhythm graph, Figure 4 is a light cycle and biorhythm graph of the gill (Gill) according to a preferred embodiment of the present invention, Figure 5 is a light cycle of the digestive duct (Digestive duct) according to a preferred embodiment of the present invention Star light response and biorhythm graph, Figure 6 is a light cycle and light rhythm graph of the hepatopancreas (Hepatopancreas) according to a preferred embodiment of the present invention.

Referring to the bar shown in Figures 2 to 6, it can be seen that Melatonin is the highest expression in the eye bottle (Eyestalk) of Figure 3 first. In particular, it was confirmed that the highest amount of secretion was maintained in the DD experiment (Continued dark) according to the light cycle, the highest Melatonin activity, and on the contrary, the secretion was kept low in the LL experiment (Continued light). 2 to 6, it can be seen that Melatonin is secreted the highest at 1 am (18h).

These results show a similar secretion pattern to mammals and fish, but at night time, Melatonin is secreted mainly from ophthalmic bottles, so the effects of light on all tissues of the cleaner shrimp (brain, eye disease, gills, liver pancreas, digestive tract). It can be concluded by receiving sensitively.

(2) Light response and biorhythm analysis (Melatonin) by light source

FIG. 7 is a light response and biorhythm graph for each light source of a brain according to a preferred embodiment of the present invention, and FIG. 8 is a light response and biorhythm graph for each light source of an eyetalk according to a preferred embodiment of the present invention. 9 is a light response and biorhythm graph of gills (Gill) according to a preferred embodiment of the present invention, Figure 10 is a light response and light response of the hepatopancreas (Hepatopancreas) according to a preferred embodiment of the present invention FIG. 11 is a biorhythm graph, and FIG. 11 is a light response and biorhythm graph for each light source of a digestive duct according to a preferred embodiment of the present invention.

Referring to FIGS. 7 to 11, unlike FIG. 2 to FIG. 6, in which photoreactions and biorhythms are analyzed for light cycles, photoreaction and biorhythms are analyzed for light sources. Melatonin is mainly used for brain and eye diseases. (Eyestalk), and the tendency to increase the amount of Melatonin secretion at night rather than daytime. In particular, unlike the wavelengths of other light sources, the most amount of Melatonin secreted in the wavelength of the red LED light source is confirmed.

As a result of analysis, Melatonin is highly expressed in brain and ophthalmic bottles, while secretion is maintained at high red LED wavelength, while Melatonin is relatively low at green LED, blue LED, and UV LED wavelength. Except for the UV LED, there is no difference in the amount of secretion according to the intensity of the LED, but the stronger the intensity of the UV LED was confirmed that the secretion decreases.

Accordingly, it can be seen that the secretion of Melatonin is mainly made in the brain and eye bottles. Since the red LED is dark enough to be memorized, it can be seen that the red LED light source promotes Melatonin secretion of cleaner shrimp and enhances immunity.

However, Cont. Shown in FIGS. 7 to 11 is a control, R0.5 is a red LED (0.5W / m ² ), R1.0 is a red LED (1.0W / m ² ), and G0.5 is a green LED (0.5). W / m ² ), G1.0 is green LED (1.0W / m ² ), B0.5 is blue LED (0.5W / m ² ), B1.0 is blue LED (1.0W / m ² ), UV0. 2 means UV LED (0.2 W / m ² ), UV 0.4 means UV LED (0.4 W / m ² ).

(3) Light response and biorhythm analysis for each light source (Clock gene-Cry1, Per2)

FIG. 12 is a graph of light response and clock gene Cry1 expression biorhythm of each brain according to a preferred embodiment of the present invention, and FIG. 13 is a light source of eye bottles according to a preferred embodiment of the present invention. Optical response and clock gene (Cry1) expression biorhythm graph, Figure 14 is a light response and clock gene (Cry1) expression biorhythm graph of the liver pancreas (Hepatopancreas) according to a preferred embodiment of the present invention.

FIG. 15 is a graph illustrating light response and a clock gene (Per2) expression biorhythm according to light sources of a brain according to a preferred embodiment of the present invention, and FIG. 16 is a light source of eye bottles according to a preferred embodiment of the present invention. Optical response and clock gene (Per2) expression biorhythm graph, Figure 17 is a light source and clock gene (Per2) expression biorhythm graph of hepatopancreas according to a preferred embodiment of the present invention.

12 to 17, it can be seen that the secretion of the clock gene is mainly made in the ophthalmic bottle, the secretion amount is maintained high at the green LED wavelength, while the red LED is recognized as dark brightness secretion is low at the red LED wavelength It was maintained and secretion was low at the blue LED and UV LED wavelengths.

Except for the UV LED, there was no difference in the amount of secretion according to the intensity of the LED, the secretion amount was decreased as the intensity of the UV. For reference, there was no significant difference in eye diseases at night time without light.

12 to 17 is a control, R0.5 is a red LED (0.5W / m ² ), R1.0 is a red LED (1.0W / m ² ), G0.5 is a green LED (0.5) W / m ² ), G1.0 is green LED (1.0W / m ² ), B0.5 is blue LED (0.5W / m ² ), B1.0 is blue LED (1.0W / m ² ), UV0. 2 means UV LED (0.2 W / m ² ), UV 0.4 means UV LED (0.4 W / m ² ).

(4) Optical response and salinity stress analysis by tissue

FIG. 18 is a tissue-specific optical response and salt stress analysis (NKA) graph according to a preferred embodiment of the present invention. Referring to FIG. 18, as the time of exposure to salinity changes (0day → 1day → 2day → 3day), NKA is decreased in low salinity and high salinity, especially in 40psu (115% SW), which is a high salinity experiment. It became.

Significantly high levels are maintained by the red and green LEDs because the red and green LEDs are suitable light sources for reducing oxidative stress. In particular, it is thought that the decrease of ion control ability occurred due to the decrease of NKA activity of Gill.

19 is a tissue-specific optical response and salt stress analysis (SOD) graph according to a preferred embodiment of the present invention. Figure 19- (a) shows the salinity stress (SOD) of the liver exposed to different concentrations of salt (Liver), Figure 19- (b) shows the gills (Gill) in different concentrations of salinity The salinity stress according to the exposure is analyzed (SOD), and FIG. 19- (c) shows the salinity stress according to the exposure of muscles to different concentrations of salinity (SOD).

According to FIG. 19, the salinity stress is expressed in the liver (Liver), the secretion amount is increased in the low salt (25 (70% SW)) and high salt (40 (115% SW)) than the control (Cont.). Secretions remain low at the red and green LED wavelengths, while secretions are high at the blue and UV LED wavelengths.

Therefore, it shows strong resistance to low salinity rather than high salinity, and red LED and green LED can be seen as a suitable light source for reducing oxidative stress.

20 is a tissue-specific optical response and salt stress analysis (CAT) graph according to a preferred embodiment of the present invention. Figure 20- (a) shows the salinity stress (CAT) of the liver exposed to different concentrations of salinity (Liver), Figure 20- (b) shows the gills (Gill) in different concentrations of salinity The salinity stress according to exposure is analyzed (CAT), and FIG. 20- (c) shows the salinity stress according to the exposure of muscles to different concentrations of salinity (CAT).

According to FIG. 20, the salinity stress was higher in the liver, the secretion amount was increased in the low and high salinity than the control, and the same trend as the SOD secretion was observed, but the expression (change) range was smaller than the SOD. Accordingly, it can be seen that the red LED and the green LED are suitable light sources for reducing oxidative stress.

18, 19 and 20, Cont. Is a control, R0.5 is a red LED (0.5W / m ² ), R1.0 is a red LED (1.0W / m ² ), G0.5 is green LED (0.5W / m ² ), G1.0 is green LED (1.0W / m ² ), B0.5 is blue LED (0.5W / m ² ), B1.0 is blue LED (1.0W / m ² ) , UV0.2 means UV LED (0.2W / m ² ), UV0.4 means UV LED (0.4W / m ² ).

(5) Activity of antioxidant enzymes, stress and immunomarker in cleaner shrimps according to water temperature change

21 is an activity indicator of the cleaner shrimp by the water temperature change in accordance with a preferred embodiment of the present invention.

Figure 21- (a) shows the SOD after the cleaner shrimp were incubated for two weeks in a 26 ° C water bath and then transferred to different baths with 21 ° C, 26 ° C and 31 ° C water temperatures. 21- (b) shows the following CAT after 26 ° C cleaner shrimp was incubated for 2 weeks in a 26 ° C water bath and then transferred to different baths with 21 ° C, 26 ° C, and 31 ° C water temperatures. 21- (c) shows the LPO after the cleaner shrimp was incubated for two weeks in a 26 ° C. water bath and then transferred to different baths having 21 ° C., 26 ° C. and 31 ° C. water temperatures. 21- (d) shows Melatonin after the cleaner shrimp was incubated for 2 weeks in a 26 ° C. water bath and then transferred to different baths having 21 ° C., 26 ° C. and 31 ° C. water temperatures.

That is, the SOD of FIG. 21- (a) and the CAT of FIG. 21- (b) indicate the degree of antioxidant activity according to the water temperature change, and the LPO of FIG. 21- (c) indicates the degree of oxidative stress according to the water temperature change. As the water temperature changes, the value decreases. In addition, Melatonin of Figure 21- (d) indicates the degree of immunity with Melatonin activity according to the change in water temperature, the better the value increases as the water temperature changes.

21 is a fluorescent lamp (control), R0.5 is a red LED (0.5W / m ² ), R1.0 is a red LED (1.0W / m ² ), G0.5 is a green LED (0.5) W / m ² ), G1.0 is green LED (1.0W / m ² ), B0.5 is blue LED (0.5W / m ² ), B1.0 is blue LED (1.0W / m ² ), UV0. 2 means UV LED (0.2 W / m ² ), UV 0.4 means UV LED (0.4 W / m ² ).

Ⅵ. Conclusion of the present invention

Through the experiments as described above, the physiological response of the cleaner shrimp according to various kinds of LED light source irradiation could be confirmed.

This means that by exposing the cleaner shrimp to a rapid salinity change and water temperature change environment, it was confirmed that the stress and immunity deterioration of the cleaner shrimp can be controlled by a specific light source of the LED.

In the case of the blue LED and the UV LED, it was confirmed that the stress increased due to the negative control of the bio rhythm of the cleaner shrimp, the negative control of the osmolality, and the mortality due to the salt stress increased.

On the other hand, red LEDs among red LEDs and green LEDs, in particular, are positive for controlling the biorhythm of the cleaner shrimp, which is effective in reducing stress, positive in controlling osmolality, and reduced mortality due to salt stress. It was found that it is effective in controlling the biorhythm and reducing stress of cleaner shrimp at the wavelength.

In particular, red LED has been known to increase and induce stress when irradiated with fish, but unlike shrimp, it is important to reduce the mortality of shrimp by reducing stress and increasing immunity. It is meaningful.

The above description is merely illustrative of the technical idea of the present invention, and those skilled in the art to which the present invention pertains may make various modifications and changes without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to describe the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments.

The protection scope of the present invention should be interpreted by the claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

Claims (4)

To reduce the mortality of shrimps exposed to various environments,
A first step of preparing shrimps;
A second step of stocking the shrimps in a water tank containing salt water; And
The shrimps are exposed to a condition in which the salinity of the water is any one of 25 psu, 30 psu, 35 psu, and 40 psu, or at a temperature of 21 ° C., 26 ° C. and 31 ° C. When exposed, the third step of reducing the oxidative stress of shrimps to increase the immunity by irradiating a red LED light source of 600 ~ 650nm wavelength;
In the third step,
Shrimp generated by reactive oxygen species by receiving light from eyetalk with high melatonin activity compared to brain, gill, digestive duct and hepatopancreas Reduction of oxidative stress of the melatonin (Melatonin) activity to increase the immunity due to the increase in the activity of the shrimp, characterized in that the stress reduction and shrimp farming method by using a light emitting diode light source irradiation. delete delete delete

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