KR102054194B1 - Anti-obesity composition comprising Suaeda japonica extract as effective component - Google Patents

Abstract

The present invention relates to a composition for anti-obesity containing the extract of turkey vinegar as an active ingredient, the extract of the present invention of the present invention shows the effect of reducing the body weight, subcutaneous fat tissue and visceral adipose tissue of obesity-induced animal model, subcutaneous fat tissue Increase in UCP-1 expression and increase the body temperature when exposed to the cold in the effect of promoting energy metabolism, anti-obesity composition containing the extract of the present invention as an active ingredient is anti-obesity health functional food or obesity It can be usefully used as a therapeutic agent.

Description

Anti-obesity composition comprising Suaeda japonica extract as effective component}

The present invention relates to a composition for anti-obesity containing the extract of turkey vinegar as an active ingredient.

Obesity is a condition in which excessive body fat is accumulated in the body, and in the case of obesity, people usually gain a lot of weight. do. Excessive intake of nutrients relative to energy consumption over long periods of time leads to obesity caused by energy imbalances, as well as a combination of hormonal changes, heredity, mental health problems and socioeconomic factors. As the obesity population is increasing all over the world, it has recently been identified as an important factor causing adult disease, and the severity of obesity has emerged as an important problem.

In order to prevent and improve obesity and maintain a healthy weight, you need to take care of your life through balanced diet and regular exercise. In particular, once the fat cells are made, they can be reduced in size, but they are impossible to remove naturally, so they remain permanently in the human body.

In order to prevent, improve or treat obesity, a method of taking drugs such as reducing calorie intake, increasing activity and exercise amount, and reducing fat absorption are used.

Current treatments for obesity include fenfluramine, which inhibits the serotonin nervous system, ephedrine and caffeine via the noradrenaline nervous system, and sibutramine and pancreatic lipase, which simultaneously act on the serotonin and noradrenaline nervous system, to reduce fat absorption. Drugs such as start. However, among the previously used drugs, fenfluramine has been banned due to side effects such as primary pulmonary hypertension and heart valve lesions, and other drugs are also used for patients with heart failure or kidney disease due to problems such as blood pressure reduction or lactic acidosis. There is a problem that can not be. Therefore, development of a substance for preventing, improving or treating obesity with less side effects is required.

Recently, as a methodological study for relieving obesity, the brown adipogenesis of white fat cell tissue, which is a report of fat storage, has become a hot topic. Adipose tissue is known to have white and brown fat. In general, white fat, which mainly consists of abdominal obesity, which causes harmful obesity or metabolic syndrome, functions as a fat storage, storing excess energy sources in the form of fat and supplying it to other organs as needed, and in a relatively small amount. It is located in local parts of the body (between the shoulder blades, around the kidneys, behind the neck), and brown fat, which contributes to maintaining body temperature by inducing fever using a fat energy source, is responsible for the function of fat consumption. In other words, the activation of brown adipose tissue that eliminates excess fat energy has emerged as a new way of resolving obesity. Recently, research on brown adipose tissue has been actively conducted. Brown adipocytes specifically express UCP-1 (uncoupling protein) in the inner membrane of the mitochondria, through the associated pairing reaction, which generates heat instead of ATP, a bioenergy form, contributing to energy consumption. This suggests that it is difficult to endure the cold systemically or coldly when interpreted clinically, and that abnormal hypothermia is a physiological condition that is not easy to relieve obesity. In other words, the tissue formation size of brown fat is poor or the activation is low, so the efficiency of energy consumption through heat generation is low. In addition, the brown adipose tissue has a large number of sympathetic nerves are more lipolysis and fatty acid oxidation is bound to be greater.

Recently, the traits of white adipocytes are reduced by stimulation such as exercise or cold in the white adipocytes, which are harmful to health, but have the characteristics of brown adipocytes and increase the expression of UCP-1. It is called a cell. These beige adipocytes are known to be beneficial for obesity and metabolic diseases, similar to the brown adipocytes found in mice. In addition, since most of the beneficial brown fat cells found in humans are known as beige fat cells, there is increasing interest in inducing the conversion or differentiation of unhealthy white fat cells into beige fat cells, which are relatively beneficial to health.

Therefore, the regulation of UCP-1 protein activity and the production of beige (or brown) adipocytes have been the subject of major research related to obesity, and the research on this has been done, but it is still inadequate.

On the other hand, Chil-myeoncho is a representative salt vegetation of pearl tidal flat, soaked in seawater and distributed in high soil salt concentration. Stems grow straight up to about 10-50cm in height, and branches do not spread much from the back. Alternately running leaves have fleshy short clubs or linear pointed ends. Along with the stem, the entire body is reddish to color the mud flat. In August-September, flowers of small miscellaneous flowers bloom on the top of stems or branches. There is no stalk, it is green at first, but gradually turns purple. The calyx leaves are deeply divided into 5 branches, with 5 stamens and 2 stamens. Poaceae is wrapped with calyx and contains one seed.

Chil-myeoncho is resistant to flooding and grows mainly in the lower part of the basin where it is periodically flooded in the tidal flats and in the lower part of the river in brackish areas. In the early reclaimed land, it is one of the pioneer plants, and prefers to be humid, and grows mainly in the mud flats and in the low soil where the soil moisture is high. However, over time after reclamation, the soil dries up and most of it disappears.

On the other hand, Korean Patent No. 1261029 discloses a diabetic prevention or improvement composition including a turkey vinegar, but does not disclose a composition for anti-obesity containing the turkey vinegar of the present invention as an active ingredient.

The present invention was derived by the above requirements, to provide a composition for anti-obesity containing the extract of turkey vinegar as an active ingredient, the composition reduces body weight and fat tissue in obesity-induced animal model, blood leptin The present invention was completed by reducing the concentration and confirming increased expression of UCP-1 in subcutaneous fat.

In order to solve the above problems, the present invention provides a health functional food composition for the prevention or improvement of obesity containing the extract of the turkey vinegar as an active ingredient.

In another aspect, the present invention provides a pharmaceutical composition for the prevention or treatment of obesity containing the extract of turkey vinegar as an active ingredient.

The present invention relates to an anti-obesity composition containing an extract of turkey vinegar as an active ingredient, the extract of the present invention is a high-fat diet in obesity-induced animal model to reduce weight without difference in feed intake, fat tissue and fat cells Decreases the area of, decreases the expression of FAS, leptin, PPAR-γ and AP2 in epididymal adipose tissue, significantly enhances the expression of UCP-1 in subcutaneous fat tissue, and increases body temperature by promoting metabolism There is.

Figure 1 is a result of confirming the weight loss effect by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, Garcinia cambogia ) is a positive control group orally administered 245mg / kg / day, HFD-SJE_100 is a high-fat diet and a group of 100mg / kg / day oral administration, HFD-SJE_200 is a high-fat diet 200mg / kg Oral administration of / day.
Figure 2 shows the results of confirming the change in dietary intake (a) and the dietary efficiency (b) by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract. +++ means that the dietary efficiency of the high fat diet group is significantly increased compared to the normal diet group, and *, **, *** are significantly reduced the dietary efficiency of the extract administration group compared to the high fat diet group. +++ is p <0.001, * is p <0.05, ** is p <0.01, and *** is p <0.001.
Figure 3 is a result of confirming the change in weight of fat tissue by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract. +++ means a significant increase in the weight of adipose tissue in the high-fat diet group compared to the normal diet group, *, ** indicates a significant decrease in the weight of adipose tissue in the extract group compared to the high-fat diet group. +++ is p <0.001, * is p <0.05, and ** is p <0.01.
Figure 4 is a result of confirming the change in the area of fat cells by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract. +++ means that the fat cell area of the high fat diet group was significantly increased compared to the normal diet group, and *** indicates that the fat cell area of the extract administration group was significantly reduced compared to the high fat diet group. +++ is p <0.001 and *** is p <0.001.
5 is a result of confirming the change in weight of liver, kidney and spleen by the extract of turkey vinegar in the animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract. +++ means that the long-term weight of the high-fat diet group was significantly increased compared to the normal diet group, * means that the liver weight of the extract-treated group was significantly reduced compared to the high-fat diet group, + ++ is p <0.001 and * is p <0.05.
Figure 6 is a result of confirming the concentrations of leptin (a) and adiponectin (b) in the blood by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract.
Figure 7 is a result of confirming the gene expression change in the adrenal adipose tissue caused by the extract of turkey vinegar in animal models in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract.
8 is a result of confirming the change in the expression of UCP-1 in subcutaneous fat tissue by the extract of turkey vinegar in an animal model in which obesity is induced by a high fat diet. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract.
9 is a result of confirming the effect of body temperature rise by the extract of chil-cho in an obese animal model induced by high fat diet when exposed to low temperature. NCD is a normal diet group, HFD-CTL is a high-fat diet group, induction of obesity, HFD-GK_245 is a high-fat diet, a positive control group orally administered Garcinia cambogia 245mg / kg / day, HFD- SJE_100 is a high-fat diet with oral administration of turkey extract 100mg / kg / day, and HFD-SJE_200 is a high-fat diet with oral administration of 200mg / kg / day of turkey extract.

The present invention relates to a health functional food composition for the prevention or improvement of obesity containing the extract of turkey vinegar as an active ingredient.

In one embodiment of the present invention, the turkey vinegar extract is extracted using water, a lower alcohol of C ₁ to C ₄ or a mixture thereof as a solvent, preferably to extract using ethanol as a solvent, but is not limited thereto. Do not.

The turkey extract increases the expression of UCP-1 in subcutaneous fat, inducing the formation of beige adipocytes from white fat. Beige fat cells are cells that perform functions similar to brown fat cells in white adipose tissue, and consume energy.

In the dietary supplement composition according to an embodiment of the present invention, the dietary supplement composition can inhibit the increase in weight or accumulation of fat.

In the dietary supplement composition according to an embodiment of the present invention, the dietary supplement composition is fatty acid synthase (FAS), leptin, PPAR-γ (peroxisome proliferators-) in the white adipose tissue around the epididymis It is possible to reduce the expression of activated receptror-γ) and adipocyte protein 2 (AP2), increase the expression of uncoupling protein-2 (UCP-2), and increase body temperature by promoting metabolism.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). In general, in the preparation of food or beverages, the nutraceutical composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of dietary supplement. Examples of foods to which the health functional food composition may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products, including ice cream, various soups, drinks, tea Drinks, alcoholic beverages, vitamin complexes, and the like, and include all health foods in the conventional sense.

In addition, the nutraceutical composition of the present invention may be prepared as a food, in particular a functional food. Functional foods of the present invention include ingredients that are commonly added in food production, and include, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when prepared with a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates can be monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g. dextrins, cyclodextrins, etc.) or sugar alcohols (e.g. , Xylitol, sorbitol, erythritol and the like). The flavourant may be a natural flavourant (eg, taumartin, stevia extract, etc.) and a synthetic flavourant (eg, saccharin, aspartame, etc.).

Various nutritional supplements, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid The carbonation agent etc. which are used for a drink can be contained further. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention relates to a pharmaceutical composition for the prevention or treatment of obesity, containing the extract of turkey vinegar as an active ingredient.

In one embodiment of the invention, the pharmaceutical composition may be prepared in capsules, powders, granules, tablets or pills, but is not limited thereto.

The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier in addition to the active ingredient, and such carriers are commonly used in the preparation of lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, Calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals Oils and the like, but are not limited thereto. In addition to the above components, the pharmaceutical composition of the present invention may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences, 19th ed., 1995.

Suitable dosages of the pharmaceutical compositions according to the present invention may be variously prescribed by such factors as the formulation method, mode of administration, age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion and response to the patient. Can be.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered topically to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration and the like.

The pharmaceutical composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the inhibition and treatment of obesity.

The concentration of the active ingredient included in the composition of the present invention can be determined in consideration of the purpose of treatment, the condition of the patient, the period of time, etc., and is not limited to a specific range of concentration.

The pharmaceutical compositions of the present invention may be prepared in unit dosage form by formulating with a pharmaceutically acceptable carrier or excipient according to methods which can be easily carried out by those skilled in the art. It can be prepared by incorporation into a dose container. In this case, the formulation may be prepared in any one formulation selected from injections, creams, patches, sprays, ointments, warnings, lotions, linings, pastas, and cataplasmas, and may further include dispersants or stabilizers. have.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples and Examples. These preparations and examples are only intended to explain the present invention more specifically, it is apparent to those skilled in the art that the scope of the present invention is not limited thereto.

Production Example  One. Turkey  Preparation of Extract

After drying, 15 liters of 70% (v / v) ethanol aqueous solution was added to 1 kg of crushed turkey vinegar, followed by stirring. The mixture was refluxed at 85 ° C. for 3 hours using a heating mantle (MS-DM609 / 20L). The filtrate was then filtered through Whatsman filter paper No. 5A, concentrated under reduced pressure with a rotary vacuum evaporator (Rotavapor R-121, Buchi, Switzerland), and the concentrate was freeze-dried (PF-10 / ALPHA 1-2LD, Germany). Into a chamber temperature of −45 ° C., and dried under a vacuum pressure of 10 Torr for 72 hours to obtain 112 g of N. chinensis extract powder. The obtained turkey extract powder was used as a sample for the experiment while frozen storage, and suspended in physiological saline for the administration of the mouse during the experiment.

Example  1. Experimental animal model design

For this experiment, 8-week-old male C57BL / 6J mice were fed from Jackson Laboratories (Bar Harbor, ME, USA) for 2 weeks at constant temperature (21 ± 2 ° C), humidity (50 ± 5%), and 12-hour intervals After adapting to the environment with light and shade control (light on 07: 00 ~ 19: 00), randomly fed general diet, high-fat diet, high-fat diet + Garcinia cambogia extract treated group, and high-fat diet + turkey vinegar extract 100mg / kg The treatment group, high-fat diet + Chil-myeoncho extract 200mg / kg treatment group was divided into five groups of 10 each.

High-fat diets to induce obesity (Rodent Diet D12492, Research Diet, New Brunswick, NL, USA) consist of 60% fat, 20% protein, and 20% carbohydrates, and feed (AIN-76A, Research Diet, New Brunswick) , NL, USA) consists of 11.5% fat, 20.8% protein and 67.7% carbohydrates.

High fat diet and extracts were administered orally to 10-week-old mice at the same time every day for a total of 9 weeks, with Garcinia cambogia extract (GK) of 245 mg / kg / day and turkey herb extract of 100 mg / kg / day and 200 ㎎ / ㎏ / day was administered, the normal diet group and high fat diet group orally administered the same amount of saline.

Body weight and dietary intake were measured and recorded every week at regular times, and the animals were bred for 15 hours after sacrifice. Blood was collected by cardiac puncture method, and plasma was separated by centrifugation at 3000rpm and 4 ° C for 15 minutes within 30 minutes after blood collection to conduct blood biochemical tests. The separated plasma was stored at minus 70 ° C. Used. Organs and adipose tissues were extracted immediately after blood collection, weighed, and stored for RNA experiments in RNA stabilized solution, RNA was extracted within a week, and the rest was quenched with liquid nitrogen and stored at -70 ° C.

Example  2. In animal models induced obesity by high fat diet Turkey  Identify the effects of extracts on your weight

Body weight, dietary intake and dietary efficiency of the five groups of Example 1 were measured.

Body weight and dietary intake were measured at the same time each week, and daily average dietary intake was calculated by dividing the measured intake by 7 days. The food efficiency ratio (FER) was calculated by the following equation.

[Equation 1]

FER = [Total Weight Gain / Total Dietary Intake] × 100

As a result, as shown in Figure 1 it was confirmed that the body weight of the high-fat diet mouse was significantly increased compared to the normal diet mouse, in the group orally administered the turkey extract (SJE) after 2 weeks of administration of the weight gain effect It was confirmed that the decrease.

In addition, as shown in Figure 2, there was no significant difference in dietary intake between groups, but it was confirmed that the dietary efficiency decreased.

Example  3. Obesity-induced animal model in high fat diet Turkey  Determine the effect of extract on adipose tissue

The adipose tissue extracted in Example 1 may be abdominal subcutaneous fat, epididymal adipose tissue, kidney adipose tissue, and intestine adipose tissue depending on the location. Each weight was measured by dividing by.

As a result, as shown in Figure 3, the weight of the adipose tissue was increased by the high fat diet, it was confirmed that the visceral and subcutaneous fat tissue was reduced compared to the high fat diet group, when the SJE administration, especially abdominal subcutaneous fat The reduction effect was the greatest.

The extracted white adipose tissue around the epididymis was cut to 6 ㎛ thickness to measure the size of fat cells, and then subjected to H & E or Oil-red-O staining and stained with an optical microscope (Olympus BX51, Olympus Optical Co., Japan). Twenty fat cells in the affected area were measured and averaged using an image analyzer (Image-Pro Plus 5.0, Media Cybernetics, Silver Spring, MD, USA).

As a result, as shown in Figure 4, the area of fat cells increased by the high fat diet was reduced by administering the extract of chinensis.

On the other hand, as shown in Figure 5 as a result of measuring the weight of the respective organs extracted in Example 1, the weight of the liver during administration of the extract of the tannincho showed a tendency to decrease slightly, the weight of the kidney and spleen did not differ. .

Example  4. In animal model induced obesity by high fat diet Turkey  Changes of Adipokine in Blood by Extracts

The blood leptin and adiponectin contents from the blood collected in Example 1 were analyzed using an ELISA kit (R & D Systems, Minneapolis, MN, USA).

As a result, the concentration of leptin in the blood increased by high-fat diet was decreased when the extract of chile was extracted (Fig. 6 (a)), but the concentration of adiponectin was not changed by the high-fat diet, and it was a high-fat diet and garcinia cambogia (GK). ), But increased in the high-fat diet, and the administration of the extract of the turkey vinegar, the concentration change by the extract of the turkey vinegar did not appear (Fig. 6 (b)).

Example  5. High Fat By diet  Obesity-induced Animal Models Turkey  Changes in Gene Expression in Adipose Peripheral Adipose Tissue by Extracts

Using the epididymal adipose tissue extracted in Example 1, we analyzed the effect of the extracts from the extracts of Mt.

After epididymal adipose tissue is homogenized, total RNA is extracted using TRI reagents (Sigma, St. Louis, MO, USA), and treated with DNase I (Life Technologies, Grand Island, NY, USA) to process DNA. (chromosomal DNA) was removed.

The extracted RNA was synthesized using SYBR Green one-step qRT-PCR kit (Invitrogen, Carlsbad, CA) and Light Cycler (Roche, Indianapolis, IN) according to the method of Boden (Diabetes, 2005, 54: 3458-3465). Expression level was confirmed.

As a result, as shown in Figure 7, the expression of AP2, FAS, leptin, SREBP1c and PPAR-γ was increased by a high fat diet, it was confirmed that the decrease by the administration of the extract of chile. The expression of UCP-2 was reduced by the high fat diet, but showed a slightly increased pattern when the turkey extract was administered. UCP is known as UCP-1, 2 and 3, and is located inside the mitochondrial membrane. It is used to produce heat instead of synthesizing hydrogen ions generated by oxidative phosphorylation into ATP. By playing a role, it is known to increase the metabolic rate and fat removal effect.

Example  6. In an animal model of obesity induced by a high fat diet Turkey  In subcutaneous fat by extract UCP -1 expression change

Using the subcutaneous fat tissue extracted in Example 1, we analyzed the effect of the extract from the turkeys on the expression of UCP-1 gene in mice with obesity induced by high fat diet.

RNA extraction and polymerase chain reaction (PCR) were carried out in the same manner as in Example 5.

As a result, as shown in FIG. 8, the expression of UCP-1 in subcutaneous fat tissue was decreased by high fat diet, but in the group administered with the high fat diet, the expression of UCP-1 was significantly enhanced compared to the general diet group. It was confirmed that it was done. UCP-1 is a brown fat specific marker that plays an energy consuming role. Increasing the expression of UCP-1 in the subcutaneous fat, a white adipose tissue, means that beige fat cells, which act as brown fat, form in the white fat.

Example  7. In an animal model with obesity induced by a high fat diet Turkey  Body temperature increase effect by extract

Changes in body temperature with extract administration time (30, 60, 90, 120, 180 minutes) in a cold chamber maintained at 10 ° C. at the end of 8 weeks of high fat diet and extract administration in the animal model of Example 1 Was confirmed by rectal temperature measurement. The temperature was measured using a Thermalert model TH-8 temperature monitor and the probe was placed in the rectum 2.5 cm deep and timed within 30 seconds per horse.

As a result, as shown in Figure 9, 30 minutes after the administration of the extract, there was no difference in body temperature, but after 60 minutes, the body temperature of the turkey vinegar extract administration group was higher than that of the normal diet group and the high fat diet group, and the turkey vinegar extract 100mg / kg administration group The synergistic effect of body temperature was excellent when exposed to cold compared to the 200 mg / kg administration group of the turkey vinegar extract. The increase in body temperature when exposed to low temperature means that the energy metabolism is active, and the anti-obesity effect was determined by the increased energy metabolism.

Claims (5)

Decreased expression of adipocyte protein 2 (AP2), fatty acid synthase (APS), and peroxisome proliferators-activated receptor-γ genes increased by high-fat diet without changes in blood adiponectin content, which contains ethanol extract as turkey active Health functional food composition for inhibiting fat synthesis through. delete The method of claim 1, wherein the ethanol extract of turkey vinegar increases the expression of UCP-1 in subcutaneous fat to induce the formation of beige adipocytes, characterized in that it does not change high adiponectin content in blood A dietary supplement for inhibiting fat synthesis by reducing the expression of adiocyte cell 2 (AP2), fatty acid synthase (FAS), and peroxisome proliferators-activated receptor-γ (PPAR-γ) genes increased by diet. delete delete

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