KR102038366B1 - Cosmetic composition for improving acne skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne skin. In detail, the present invention is applied to the skin without irritation to provide an excellent acne skin improvement effect and at the same time help to normalize acne skin. Thus, the cosmetic composition applied on the skin effectively improves acne skin by simultaneously eliminating comedonal acne and pustular acne.

Description

Cosmetic composition for improving acne skin {COSMETIC COMPOSITION FOR IMPROVING ACNE SKIN}

The present invention relates to a cosmetic composition for improving acne skin.

Acne refers to chronic, refractory and inflammatory skin disorders that occur in the hair follicles and sebaceous glands around the hair follicles, and the exact cause of acne is unknown.

The causes of these acne can be divided into several ways. One cause is the excessive secretion of sebum. Sebum is a substance secreted by the sebaceous glands, and in normal amounts it acts to prevent harmful substances outside the skin from penetrating keratinocytes. However, under puberty, before and after menstruation, early pregnancy, contraceptives, and stress, the human body shows a different hormonal balance than normal. This altered hormonal balance stimulates sebaceous glands, which induces an oversecretion of sebum. In addition, sebum secretion may be genetically increased and may be increased by changes in external temperature or skin temperature caused by poor eating habits. Another cause is infection of the pores. Excessively secreted sebum increases the pH of the skin surface and dissolves the lipids of the skin, thereby lowering the defense of the skin itself. On the surface of the skin where pH is high, the causative agent of acne becomes easy to proliferate, and the acne proliferating acne causes purulent inflammation through weakened skin.

In general, acne can be divided into four stages according to symptoms. The first stage is scleroderma, which causes the sebum overly secreted to accumulate in the pores around the sebaceous glands, forming a scrim. Stage 1 scleroderma acne acne lesion is an early mild stage, but it is not good for cosmetics is a skin concern. Good care at this stage can also inhibit the development of inflammation. In the second stage papule acne stage acne bacteria are multiply inflammatory reactions occur red acne, which is called papules, if not properly managed can cause scars. In the third stage purulent acne, the inflammatory response of the red acne becomes more severe, and pus inside is formed. This is called pustules, and at this stage, scars may remain even after treatment. The final stage 4 nodular acne is the stage where the pus ruptures in the skin and causes more inflammation around the acne area, which forms a lump that is the size of a finger. This is called a nodule. Can also be prolonged.

Treatment of acne includes topical formulations and oral medications, and surgical treatments such as laser and dermabrasion that have recently been performed. Topical application therapy uses antibacterial agents such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, which is known as the causative agent of acne, or for benzoyl peroxide to dissolve cotton wool. peroxide) is used as external ointment type. However, azelaic acid is difficult to formulate, in the case of salicylic acid is classified as a preservative in the country there is a problem that can not contain more than 0.5% in the formulation. In the case of benzoyl peroxide, there is a problem that it cannot be used as a cosmetic raw material in Korea. Oral drug therapy uses retinoids and corticosteroids to inhibit sebaceous gland function and inhibit hair follicle wall destruction. These preparations have side effects such as skin rash, dry skin, and allergies may occur depending on the individual.

Also, the problem of acne and acne and other skin diseases that are already developed and advanced is that the external preparations used directly on the skin are more effective considering various aspects such as the speed and economical effects. As an example of the external preparation, it can be used for a long time and less damage to normal skin than the therapeutic drug acne skin cosmetics have been presented in various aspects, but after use, it causes skin side effects with therapeutic effects such as skin keratin occurs or consumers There are not many cosmetic ingredients that can provide the desired medical effect.

Retinol, on the other hand, is a type of vitamin A that plays an important role in maintaining the epidermal cells of the skin. For example, retinol is known to have an effect of enhancing collagen synthesis of the skin and promoting turnover of the stratum corneum to promote cell production. However, despite these effects, retinol causes skin irritation even when used in small amounts on the skin, and the retinol itself is very unstable and denatures or decomposes over time, reducing the titer and reducing the effect. The use of is extremely limited. In an attempt to solve this problem, there have been many attempts to add other ingredients together with retinol, but only with insufficient effects.

The present inventors conducted an in-depth study on the cosmetic raw material for acne skin containing deoxycholic acid. As a result, the present invention was completed by discovering that the nano-carrier including the glycine-based compound and the retinol-based compound at the same time expresses excellent efficacy in improving the desired acne skin and solves the problems of the retinol described above.

It is an object of the present invention to provide a nano-carrier excellent for improving acne skin and having excellent safety for skin and a cosmetic composition comprising the same.

Still another object of the present invention is to provide a nanocarrier and a cosmetic composition containing the same, which can stably encapsulate an unstable retinol-based compound.

Still another object of the present invention is to provide a manufacturing method that can provide a very stable nano-carrier in a simple process through a complex emulsification process.

In order to achieve the above object, there is provided a nano-carrier in which an active ingredient including a dioxycholic acid, a glycine compound and a retinol compound is collected therein.

The nano-carrier according to an embodiment of the present invention may be in the form of a vesicle.

The nano-carrier according to an embodiment of the present invention may be made of a gemini-type surfactant.

The nano-carrier according to an embodiment of the present invention may include a gemini-type surfactant and polyoxyethylene sorbitan fatty acid ester.

In the nano-carrier according to an embodiment of the present invention, the polyoxyethylene sorbitan fatty acid ester may be included in an amount of 1 to 10 parts by weight based on 100 parts by weight of the gemini surfactant.

In the nano-carrier according to an embodiment of the present invention, the deoxycholic acid may be included in an amount of 1 to 100 parts by weight based on 100 parts by weight of the gemini-type surfactant.

The glycine-based compound according to an embodiment of the present invention may be included in an amount of 1 to 100 parts by weight based on 100 parts by weight of the gemini-type surfactant.

In the nano-carrier according to an embodiment of the present invention, the glycine-based compound may be one or a mixture of two or more selected from undecylenoyl glycine, C12-14 alkyldiaminoethylglycine HCI, capryloyl glycine, and the like. have.

The retinol-based compound according to an embodiment of the present invention may be one or a mixture of two or more selected from retinol, retinoic acid, retinyl retinoate, retinyl palmitate, and the like.

In order to achieve the above object, a method for producing the nano-carrier is provided.

In the method for preparing a nanocarrier according to an embodiment of the present invention, an oil phase and an aqueous phase including glycine-based compounds and retinol-based compounds are sequentially added to a gel containing deoxycholic acid, and the primary particles are prepared by emulsification. Preparing an oil-in-water emulsion formed; And injecting an oil-in-water emulsion in which the primary particles are formed on a liquid crystal emulsification phase including a gemini-type surfactant, to emulsify a high pressure.

In the method for producing a nano-carrier according to an embodiment of the present invention, the gel phase may be one in which the oil is dispersed in the form of a D-phase (Detergent phase).

In order to achieve the above object, there is provided a cosmetic composition for improving acne skin comprising the nano-carrier.

The composition according to an embodiment of the present invention may be to antibacterial propionibacterium acnes causing the acne.

The composition according to an embodiment of the present invention may be a minimum growth inhibition concentration (MIC) of less than 300ppm against acne causing bacteria.

The composition according to an embodiment of the present invention may be to inhibit sebum secretion and lipid degradation of the skin.

The composition according to an embodiment of the present invention may be formulated in softening cream, converging cream, nutrient cream, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack, etc. .

According to the present invention, as well as excellent antimicrobial effect against acne-causing bacteria, by slightly acidifying the pH of the skin surface of the acne, it enables a normal skin protection function.

In addition, according to the present invention, by effectively inhibiting sebum secretion and lipolysis of the skin, by extinguishing the speculative acne, effectively improves acne skin. In particular, the nanocarrier of the present invention effectively removes excessively secreted sebum or fatty acids decomposed therefrom, and normalizes the skin's keratinization cycle by restoring the oil / moisture balance necessary to alleviate pore keratosis.

In addition, according to the present invention, it provides a nano-carrier, that is, nano vesicles in a very simple process, which has the advantage that it can stably collect poorly soluble active ingredients.

Therefore, the nano-carrier and the cosmetic composition comprising the same of the present invention is applied to the skin without irritation to provide an excellent acne skin improvement effect and at the same time helps to normalize acne skin.

The cosmetic composition for improving acne skin according to the present invention will be described in detail below. However, unless there is another definition in the technical terms and scientific terms used at this time, a person having ordinary knowledge in the technical field to which the present invention belongs generally means The description of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

As used herein, the term "GEMINI-type surfactant" means a surfactant having two hydrophobic chains and two hydrophilic chains in one molecule, and an interface including a structure in which a hydrophilic group or its vicinity is connected with a linking group. It may also include an active agent.

In addition, the term "antibacterial" in the present specification means the ability to resist the bacteria, it means all the activities made to protect the skin from the action of the acne causing bacteria.

In addition, the term "Detergent phase Emulsification Method" in the present specification is to produce an isotropic gel phase (surfactant phase (D phase) containing water and polyhydric alcohol, and to add oil, O / D gel emulsion It refers to an emulsification method for forming an O / D emulsion by adding water to the O / D gel emulsion to change the continuous phase from the gel to water.

In addition, the term "phase inversion temperature (PIT)" in the present specification uses a composition consisting of a three-component system of water, oil and surfactant, and the hydrogen of the hydrophilic group and water of the surfactant as the temperature rises Emulsification method using the principle that the bond is reduced to reduce the hydrophilic properties.

In addition, the term "5α-Reductase" in the present specification is present in sebaceous glands, hair follicles, etc., and is an enzyme involved in reducing Testosterone to Dihydrotestosteron (DHT). By inhibiting 5α-reductase, hypersecretion of sebum can be suppressed.

The inventors of the present invention while studying the cosmetic raw material for acne skin containing dioxycholic acid, when synthesizing glycine compound and retinol compound with deoxycholic acid at the same time, synergistic effect on acne skin improvement compared to the use of dioxycholic acid alone I confirmed it. Therefore, in order to apply them to the formulation more stably, a nano-carrier including them was devised.

According to the present invention, the synergistic effect of the antibacterial effect on the acne-causing bacteria, as well as effectively inhibit the sebum secretion and lipid degradation of the skin, it is possible to provide a composition for external application to the skin to improve acne and acne skin improvement activity.

In addition, according to the present invention, according to the change in the solubility of the deoxycholic acid changes according to the pH of the skin surface, the nanocarrier including the same effectively performs the role of steep percutaneous absorption, showing that it is excellent in the desired acne skin improvement activity.

Hereinafter, the nanocarrier according to the present invention will be described.

Nano-carrier according to an embodiment of the present invention may be in the form of a vesicle.

Nano-carrier according to an embodiment of the present invention may be a collection of dioxycholic acid, glycine-based compound and retinol-based compound as an active ingredient inside the vesicle.

The nanocarrier shows an excellent antimicrobial activity against acne causing bacteria, and expresses an excellent effect on the removal of solid fatty acids. Moreover, the nanocarrier acts as a 5α-reductase inhibitor, effectively inhibiting the excessive secretion of sebum found in acne skin.

In addition, the nano-carrier to adjust the acne skin to weak acidity, to create an environment that is not suitable for acne-causing bacteria growth on the surface of the skin, and reduce skin irritation.

Nano-carrier according to an embodiment of the present invention may be formed using a Gemini type surfactant.

The nano-carrier may be formed using a lipid component (eg, phosphatidyl choline, etc.) having a packing parameter close to 1, but for more stable formulation application, it includes a gemini-type surfactant. .

In general, in case of using a lipid component having a packing parameter close to 1, the flexibility of the membrane is insufficient, but the nanocarrier prepared using the Gemini-type surfactant can capture the active ingredient more stably because of the flexibility of the membrane and the stable formation of the membrane. Can be.

The gemini-type surfactant may be an anionic surfactant.

For example, the gemini-type surfactant may be an anionic sulfate group (* -SO ₃ M, M is an alkali metal ion, such as sodium ion) at the terminal.

For example, the gemini-type surfactant may be sodium dicoyl ethylenediamine PEG-15 sulfate.

In one example, the gemini surfactant may be used in the form of a mixture comprising the sodium dicoyl ethylenediamine PEG-15 sulfate. Examples include, but are not limited to, the trade names Ceralution ^® H, Ceralution ^® F, Ceralution ^® C, etc., from Sasol.

Nano-carrier according to an embodiment of the present invention can increase the flexibility of the membrane and the stability of the nano-carrier, including the dioxycholic acid and glycine-based compounds. Moreover, some of the deoxycholic acid can be introduced to the membrane surface of the nanocarrier to effectively perform the steep role of transdermal absorption.

The dioxycholic acid and glycine-based compound according to an embodiment of the present invention may be used in an amount of 1 to 100 parts by weight, based on 100 parts by weight of the gemini-type surfactant. The dioxycholic acid and glycine-based compound may be specifically used in 5 to 80 parts by weight, and more specifically in each 10 to 50 parts by weight.

In addition, the nano-carrier according to an embodiment of the present invention can further increase the stability of the nano-carrier further comprises a polyoxyethylene sorbitan fatty acid ester.

For example, the polyoxyethylene sorbitan fatty acid ester is polyoxyethylene sorbitan oleate, polyoxyethylene sorbitan stearate, polyoxyethylene sorbitan laurate, polyoxyethylene sorbitan sesquioleate, polyoxyethylene sorbitan It may be one or a mixture of two or more selected from palmitate, polyoxyethylene sorbitan trioleate and the like.

For example, the polyoxyethylene sorbitan fatty acid ester may include a saturated fatty acid ester group.

The polyoxyethylene sorbitan fatty acid ester according to an embodiment of the present invention may be used in an amount of 1 to 10 parts by weight based on 100 parts by weight of the gemini surfactant. The polyoxyethylene sorbitan fatty acid ester may be specifically used in 3 to 10 parts by weight, and more specifically in 5 to 10 parts by weight.

Nano-carrier according to an embodiment of the present invention can be utilized in various formulations by stably collecting glycine-based compounds and retinol-based compounds that are one of the poorly soluble active ingredients. In addition, the nano-carrier including the same is applied to the acne skin, coating the skin slightly acidic. Thus, synergism is given to the improvement effect of acne skin, and help normalize acne skin can effectively suppress skin irritation.

For example, the glycine-based compound may be one or a mixture of two or more selected from undecylenoyl glycine, C12-14 alkyldiaminoethylglycine HCI, capryloyl glycine, and the like.

For example, the retinol-based compound may be one or a mixture of two or more selected from retinol, retinoic acid, retinyl retinoate, retinyl palmitate, and the like.

Nano-carrier according to an embodiment of the present invention effectively improves the problem of retinol that can cause irritation even in normal skin. Specifically, the nanocarrier and the formulation including the same can be applied to the acne skin without irritation, thereby effectively expressing the activity of the retinol.

Hereinafter, the manufacturing method of the said nanocarrier is demonstrated.

Nano-carrier according to an embodiment of the present invention can be prepared through a complex emulsification process. Specifically, the complex emulsification process may be a process in which the D-phase emulsification and total-phase emulsification are combined.

In the method for preparing a nanocarrier according to an embodiment of the present invention, an oil phase and an aqueous phase including glycine-based compounds and retinol-based compounds are sequentially added to a gel containing deoxycholic acid, and the primary particles are prepared by emulsification. Preparing an oil-in-water emulsion formed; And injecting an oil-in-water emulsion in which the primary particles are formed on a liquid crystal emulsification phase including a gemini-type surfactant, to emulsify a high pressure.

According to the manufacturing method, it is possible to form fine primary particles.

In addition, according to the production method, it is possible to use a variety of oils as well as to form a stable phase even when using a high content of oil.

By employing the complexed emulsifying process, the oil which can be used in the gel phase and the oil phase is not limited.

For example, the oil may include a hydrocarbon oil, an ester oil, a diester oil, a triglyceride oil, a carbonate oil, an alcohol oil and a silicone oil.

For example, the oil may use one or more triglyceride oils selected from caprylic / capric triglycerides, caprylic / capric / stearic triglycerides, triethyloctanoin, tricapryline, and the like. have.

At this time, the oil may be a combination of the above-described oils as well as the triglyceride oil, depending on the formulation or purpose of use.

In the above method, the step of preparing the oil-in-water emulsion in which the primary particles are formed may specifically include the following two steps.

Step 1 may be a step of emulsifying D phase by sequentially inputting an oil phase and an aqueous phase including a glycine compound and a retinol compound on a gel containing dioxycholic acid.

Step 1 may use the first composition.

For example, the first composition is a gel phase containing 0.1 to 5% by weight of dioxycholic acid based on the total weight of the first composition, 0.1 to 5% by weight of glycine-based compound and 0.01 to 0.5% by weight of retinol-based compound It may be to include an oil phase containing and an aqueous phase containing 30 to 90% by weight of water.

 For example, the first composition is a gel phase containing 0.5 to 5% by weight of dioxycholic acid based on the total weight of the first composition, 0.5 to 5% by weight of glycine-based compound and 0.01 to 0.3% by weight of retinol-based compound It may be to include an oil phase containing and an aqueous phase containing 30 to 90% by weight of water.

For example, the first composition is a gel phase containing 0.5 to 3% by weight of dioxycholic acid, 0.5 to 3% by weight of glycine-based compound and 0.05 to 0.2% by weight of retinol-based compound, based on the total weight of the first composition. It may be to include an oil phase containing and an aqueous phase containing 30 to 90% by weight of water.

In the first composition, the gel phase may be an oil dispersed in a D-phase form.

The gel phase may include an alkylene oxide adduct-type nonionic surfactant.

For example, the alkylene oxide adduct-type nonionic surfactant may be selected from one or two or more selected from ceteareth-12, vinceteareth-20, vinceteareth-25, vincetearese-30, steares-10 and steares-20. It may be a mixture.

Step 1 may be performed at 65 to 95 ° C., specifically, at 75 to 95 ° C., more specifically, at 80 to 90 ° C.

After step 1, step 2 of total phase emulsification of the O / D gel emulsion formed by D phase emulsification may be performed.

Step 2 may be performed by rapidly cooling to a temperature below the phase temperature.

Step 2 may use a second composition.

For example, the second composition may include an aqueous phase including 5 to 30 wt% of an O / D gel emulsion prepared by the preparation method and 1 to 10 wt% of a gemini surfactant based on the total weight of the second composition. It may be to include an oil phase containing.

For example, the second composition is based on the total weight of the second composition, the aqueous phase including 5 to 20% by weight of the O / D gel emulsion prepared by the preparation method and 3 to 10% by weight of the Gemini-type surfactant It may be to include an oil phase containing.

For example, the second composition may include an aqueous phase including 8 to 15 wt% of an O / D gel emulsion prepared by the preparation method and 3 to 8 wt% of a gemini surfactant based on the total weight of the second composition. It may be to include an oil phase containing.

For example, the oil phase of the second composition may further include polyoxyethylene sorbitan fatty acid ester, and the polyoxyethylene sorbitan fatty acid ester may be included in an amount of 1 to 10 parts by weight based on 100 parts by weight of the gemini surfactant. have.

The phase temperature may be 30 ° C or less, specifically 15 to 30 ° C, more specifically 20 to 30 ° C.

Through the complex emulsification process as described above, it is possible to more stably form an oil-in-water emulsion in which primary particles including dioxycholic acid, glycine-based compound and retinol-based compound are formed.

For example, the oil-in-water emulsion in which the primary particles are formed may have an average particle size (D ₅₀ ) of 1,000 nm or less.

For example, the oil-in-water emulsion in which the primary particles are formed may have an average particle size (D ₅₀ ) of 500 to 1,000 nm.

In addition, the gel phase, oil phase and water phase of the first composition and the oil phase and water phase of the second composition may further include various additives according to the purpose.

In this case, the additive may be selected from an oil, a polyol, a stabilizer, an emulsifier, a thickener, a liquid crystal film strengthening agent, a preservative, a pH adjusting agent, and the like, but is not limited thereto.

In addition, in the above production method, the oil-in-water emulsion in which the primary particles are formed may be manufactured through a step of high-pressure emulsifying, to further manufacture the atomized nanocarrier.

The high pressure emulsifying step may be performed by repeating the oil-in-water emulsion in which the primary particles are formed in a high pressure emulsifier 1 to 10 times at a pressure of 500 to 1,500 bar.

For example, the nano-carrier through the high pressure emulsification may have an average particle size (D ₅₀ ) of less than 500nm.

For example, the nanocarrier may have an average particle size (D ₅₀ ) of 100 to 450nm.

For example, the nanocarrier may have an average particle size (D ₅₀ ) of 250 to 350nm.

The solution containing the nanocarrier prepared by the above method may have a translucent or transparent appearance.

In the manufacturing method, Degassing and cooling after the high pressure emulsifying step; Of course, it may further include. The degassing and cooling may be carried out in a conventional method and the conditions "temperature", but is not limited thereto.

Hereinafter, a cosmetic composition for improving acne skin including the nanocarrier will be described.

The cosmetic composition for improving acne skin according to an embodiment of the present invention may include a nanocarrier in which an active ingredient including deoxycholic acid, a glycine-based compound, and a retinol-based compound is collected.

The cosmetic composition for improving acne skin has an excellent effect on the antimicrobial activity against acne causing bacteria propionibacterium acnes. In addition, the cosmetic composition for improving acne skin effectively inhibits sebum secretion and lipid degradation of the skin, and excellent scalp dissolving ability.

Thus, when applying the cosmetic composition for improving acne skin on the skin, by simultaneously extinguishing the scum acne and purulent acne, effectively improves acne skin.

In addition, the cosmetic composition for improving acne skin may be utilized in various formulations as it stably collects glycine-based compounds and retinol-based compounds having an active concentration or more.

In addition, the cosmetic composition for improving acne skin improves acne skin, normalizes the skin's keratinization cycle by restoring the oil / moisture balance, and enables a normal skin protection function.

In addition, the cosmetic composition for improving acne skin effectively improves the problem of retinol, and can be applied to the acne skin without irritation.

The cosmetic composition for improving acne skin according to an embodiment of the present invention may be to improve acne skin with excellent antibacterial activity against acne causing bacteria Propionibacterium acnes. Its effect corresponds to a significantly improved synergistic effect compared to the example used alone.

In the cosmetic composition for improving acne skin, the minimum growth inhibition concentration (MIC value) for the Propionibacterium acnes may be less than 300ppm.

The MIC value may correspond to the concentration of the nanocarrier, the MIC value may be specifically 0.1 to 100ppm range, more specifically 0.1 to 10ppm range.

Cosmetic composition for acne skin improvement according to an embodiment of the present invention, by inhibiting sebum secretion and lipid degradation of the skin, may be to improve acne skin.

The cosmetic composition for improving acne skin, the antimicrobial activity against acne causing bacteria can be further enhanced to implement the desired antimicrobial activity in a small amount as well as effectively inhibit sebum secretion and lipid degradation of the skin to extinguish the scum acne.

In addition, the cosmetic composition for improving acne skin does not have any side effects on the skin and does not cause precipitation or degeneration (eg, change in titer of active ingredients, discoloration, odor, etc.) even during long-term storage. Furthermore, according to the present invention, the glycine-based compound and the retinol-based compound, which are poorly soluble active ingredients, are collected and provided in the nano-carrier so as to be more stably formulated.

The cosmetic composition for improving acne skin according to an embodiment of the present invention may be formulated into various forms of dosage forms by minimizing changes in titers of effective ingredients as well as implementing a stable formulation.

For example, the cosmetic composition for improving acne skin may be formulated as a flexible cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack .

In addition, the cosmetic composition for improving acne skin according to an embodiment of the present invention may be included in an amount of 1 to 20% by weight based on the total weight of the composition, specifically 5 to 20% by weight, in terms of expressing the acne skin improvement effect. , More specifically, it may be included in 8 to 12% by weight, but is not limited thereto.

In addition, the cosmetic composition for improving acne skin according to an embodiment of the present invention contains various anti acne cosmetic raw materials or plant extracts that can give a synergistic effect to the main effect within the range of not impairing the main effect of the present invention. Of course, it may further include a variety of additives to further include or increase the formulation stability.

For example, when the cosmetic composition for improving acne skin is an emulsion formulation, it is selected from a solvent, an emulsifier, a thickener, an emulsion, an oil, a wax, a lubricant, an alcohol, a water-soluble polymer, a gelling agent, a stabilizer, an inorganic powder, a pharmaceutical, and the like. One or more additives may be used.

For example, when the cosmetic composition for improving acne skin is a solubilized formulation, one or more additives selected from a solvent, a solvating agent and an emulsifying agent may be used.

Hereinafter, the cosmetic composition for acne skin improvement according to an embodiment of the present invention will be described in more detail with reference to Examples. However, the following examples are only one reference for describing the present invention in detail, and the present invention is not limited thereto and may be implemented in various forms. In addition, the unit of the additive which is not specifically described in the specification may be wt%.

(Assessment Methods)

1. Determination of Minimum Developmental Concentration Concentration (MIC)

Propionibacterium Acnes was inoculated in BHI broth and prepared as a test bacterial solution. 0.15 ml of the test bacteria was added to 15 ml of the BHI broth (pH 6.8), followed by mixing. In addition, each of the nano-carriers prepared in Examples and Comparative Examples was used as a sample.

Each sample was placed in line 1 of a 96-well cell culture tube (96well plate) and the total amount was adjusted to 200 μl with the dilution solution. The remaining wells were put in 100 μl of the dilution solution. The mixed solution of the first row was evenly mixed, and then 100 μl of the mixed solution was added to the second row, the mixture was evenly mixed, and then 100 μl of the mixed solution was added to the third row. After incubation for 24 hours and 48 hours at 32 ° C., the growth of bacteria was measured using a spectrophotometer, and the absorbance was measured at 650 nm to confirm the growth of microorganisms, and compared with the control group. The minimum concentration showing the low proliferation of the Propionibacterium acnes strain was measured as the minimum inhibitory concentration (MIC), and the results are shown in Table 4 below.

2. Cytotoxicity Check

Using the nanocarriers prepared in Examples and Comparative Examples according to the present invention, cytotoxicity was measured in HaCaT cells and HDF-N cells. HaCaT cells were dispensed in 96 well plates at a concentration of 5 × 104 cells per well, and HDF-N cells in 96 well plates at a concentration of 6 × 103 cells per well and incubated in cell culture conditions for 24 hours. After the medium was removed and washed with DPBS, starvation was maintained for 12 hours, sample solution and fresh medium (except for media supplement) were added, and cultured for 24 hours. After removing the sample solution and the medium and washing with PBS, in order to measure the survival rate of the cells, the WST-1 reaction solution was diluted 1/10 in the medium without supplement and treated with 100 μl of each well for 1 hour. After the absorbance was measured at 450 nm, the results are shown in Table 5 below.

3. Acne improvement, sebum secretion reduction and confirmation of irritation

Twelve people with acne were allowed to use the toner prepared with the composition shown in Table 7 for one month.

The scale of inflammatory acne improvement was from 1 to 5 points, with 1 being 'no', 3 being 'normal' and 5 being 'very so'. The results are shown in Table 6, expressed as the average score of 12 people.

In addition, the acne extinction time was based on the number of days the extinction was read, and acne recurrence was based on the results after one month. The results are shown in Table 6 below.

In addition, the scale of sebaceous glands decreases from 1 point to 5 points, 1 point is ‘no’, 3 points are ‘normal’, and 5 points ‘very yes’. The results are shown in Table 6, expressed as the average score of 12 people.

In addition, the presence or absence of skin irritation was expressed as (number of irritants) / (total number of testers). The results are shown in Table 6 and FIG. 1.

4. Confirmation of Titer and Formulation Stability of Retinol Compounds

The formulation stability of each sample (Examples and Comparative Examples) and the titer of the retinol-based compound contained therein were confirmed. Each sample should be refrigerated bath (4 ± 2 ℃), frozen bath (-20 ± 2 ℃), high temperature bath (45 ± 2 ℃) and cycle chamber (Freeze-Thaw Chamber, -20 ℃ to 45 ℃ once a day). Cycles), and each sample was visually observed for phase separation at weekly intervals, and the retinol-based compound content of each sample was measured by liquid chromatography.

(Example 1 and Example 2)

To the compositions shown in Table 1 and Table 2, the nano-carrier was prepared through the following manufacturing method.

Step 1. Oil-in-water emulsion with primary particles formed (see composition in Table 1)

A was weighed and heated to 82 ° C. to completely dissolve it (phase D and PIT emulsified atmosphere composition).

B was completely dissolved, added to A, and stirred for 3 minutes with an azimixer (1000 rpm).

Thereafter, the mixture was added to C, followed by stirring for 10 minutes using an azimixer (1000 rpm), gradually cooled (˜25 ° C.), and phase-impacted with an oil-in-water emulsion in which primary particles were formed.

Step 2. Preparation of Nanocarriers (see Composition in Table 2)

A was weighed and completely dissolved at 40 ° C.

B was separately weighed into A, and emulsified first with a homomixer (3500 rpm) for 10 minutes.

After completion of the first emulsification, each C was added, stirred for 3 minutes with an azimixer (500 rpm), and then put into a high pressure homogenizer (aka: microfluidizer) and passed through a round of 500 Bar to prepare a nanocarrier ( pH 6.0, particle size of the nanocarrier (D ₅₀ ) = 300 nm.

(Comparative Example 1 to Comparative Example 3)

To the compositions shown in Table 1 and Table 2, was carried out in the same manner as in Example 1 to prepare a nano-carrier.

(Example 3 and Example 4)

Using the oil-in-water emulsion in which the primary particles prepared in step 1 of Example 1 was formed, and the composition shown in Table 3, was carried out in the same manner as in the production method of Example 1 to prepare a nano-carrier.

As shown in Table 4, it can be seen that the nano-carrier according to the present invention exhibits excellent antimicrobial activity even at a concentration of 10 ppm or less against propionibacterium acnes, which is a cause of acne. On the contrary, the nanocarriers prepared in the comparative example had no antimicrobial activity against the acne causing bacterium Propionibacterium acnes (Comparative Examples 1 and 2), or exhibited significantly lower MIC values than the nanocarriers according to the present invention. Could confirm.

As shown in Table 5, it can be confirmed that the nanocarrier according to the present invention shows a survival rate of 99.0% or more even in the above-described sample concentration. Thus, the nano-carrier according to the present invention and the cosmetic composition for improving acne skin comprising the same did not show cytotoxicity and was confirmed to have excellent stability to the skin.

As shown in Table 6, the cosmetic composition for improving acne skin comprising the nano-carrier according to the present invention exhibits excellent effects on acne improvement and disappearance, as well as effectively reduce sebum secretion, excellent overall improvement in acne It was confirmed that acne did not recur.

In particular, the cosmetic composition for acne skin improvement comprising the nano-carrier according to the present invention effectively suppresses the skin irritation caused by retinol did not cause problems of skin irritation even in long-term use.

As a result of confirming the formulation stability according to the evaluation method, it was confirmed that the nano-carrier according to the present invention is very excellent in stability, there was almost no change in the particle size of the nano-carrier even after a lapse of 4 weeks or more. On the contrary, in the case of the comparative example, it was confirmed that the stability was poor in the temperature condition of room temperature or more.

In addition, as a result of confirming the change in the titer of the retinol-based compound in the nanocarrier according to the present invention after 4 weeks of high temperature thermostat, a titer of 90% or more was confirmed. In contrast, in Comparative Example 1 and Comparative Example 2, a titer of less than 40% was confirmed.

In short, the present invention can provide a cosmetic composition for improving acne skin having excellent anti-inflammatory effect and sebum secretion effect on acne causing bacteria without causing side effects on the skin. Thus, according to the present invention can be given a pharmacological action and efficacy and effects for improving acne skin, it is expected that it can be formulated and utilized in various aspects as a new acne skin improvement cosmetic raw material.

Formulation Example 1

Toner for improving acne skin was prepared according to a conventional method with the composition of Table 7.

Formulation Example 2

To the composition of Table 8 was prepared according to the conventional method for improving acne skin essence.

In the present invention as described above has been described by specific embodiments and limited embodiments and drawings, but this is only provided to help a more general understanding of the present invention, the present invention is not limited to the above embodiments, the present invention Those skilled in the art can make various modifications and variations from this description.

Therefore, the spirit of the present invention should not be limited to the described embodiments, and all the things that are equivalent to or equivalent to the claims as well as the following claims will belong to the scope of the present invention. .

Claims (16)

A nanocarrier in which an active ingredient comprising a dioxycholic acid, a glycine-based compound and a retinol-based compound is collected inside a nanocarrier made of a gemini-type surfactant. The method of claim 1,
The nano carriers,
Nanocarrier in vesicle form. delete The method of claim 1,
The nano carriers,
The nanocarrier which further comprises a polyoxyethylene sorbitan fatty acid ester. The method of claim 4, wherein
The polyoxyethylene sorbitan fatty acid ester,
Based on 100 parts by weight of the Gemini-type surfactant, it will be included in 1 to 10 parts by weight, nano-carrier. The method of claim 1,
The deoxycholic acid,
Based on 100 parts by weight of the gemini-type surfactant, will be included in 1 to 100 parts by weight, nano-carrier. The method of claim 1,
The glycine compound,
Based on 100 parts by weight of the gemini-type surfactant, will be included in 1 to 100 parts by weight, nano-carrier. The method of claim 1,
The glycine compound,
A nanocarrier, which is undecylenoyl glycine, C12-14 alkyldiaminoethylglycine HCI, capryloyl glycine, or mixtures thereof. The method of claim 1,
The retinol-based compound,
A nanocarrier, which is retinol, retinoic acid, retinyl retinoate, retinyl palmitate or mixtures thereof. Preparing an oil-in-water emulsion in which primary particles are formed by sequentially introducing an oil phase and an aqueous phase including glycine-based compounds and retinol-based compounds on a gel containing deoxycholic acid, respectively,
Injecting an oil-in-water emulsion in which the primary particles are formed on a liquid crystal emulsification phase comprising a gemini-type surfactant, comprising the step of high-pressure emulsification. The method of claim 10,
The gel phase is,
Oil is dispersed in the form of a D phase (Detergent phase), a method for producing a nano-carrier. A cosmetic composition for improving acne skin, comprising a nanocarrier in which an active ingredient including a dioxycholic acid, a glycine-based compound, and a retinol-based compound is collected inside a nanocarrier made of a gemini-type surfactant. The method of claim 12,
The composition,
It is antibacterial to acne causing bacteria propionibacterium acne, cosmetic composition for improving acne skin. The method of claim 12,
The composition,
Minimal growth inhibition concentration (MIC) against acne causative bacteria 300 ppm or less, cosmetic composition for improving acne skin. The method of claim 12,
The composition,
To suppress sebum secretion and lipid degradation of the skin, cosmetic composition for improving acne skin. The method of claim 12,
The composition,
A cosmetic composition for improving acne skin, which is formulated as a softening cream, astringent makeup cream, nutrient cream, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack.