WO2023109290A1 - Composition containing microbial oil and vegetable oil and use thereof - Google Patents

Abstract

The present invention provides an oil composition. The oil comprises a microbial oil and a vegetable oil. Calculating on the basis of the total weight of the composition, the composition comprises at least 0.5 wt% of the oils. The present invention further relates to a use of the oil composition in enhancing a skin barrier function and/or in preparing a drug and/or an external skin agent which enhances a skin barrier function.

Description

Composition comprising microbial oil and vegetable oil and application thereof technical field

The invention belongs to the field of cosmetics, and in particular relates to a composition containing microbial oil and vegetable oil and its application in skin barrier function.

Background technique

The skin is the largest organ in the human body, covering the entire body surface and playing an important role as a barrier. In a broad sense, the skin barrier function not only refers to its physical barrier function, but also includes skin pigment barrier function, nerve barrier function, immune barrier function and many other aspects related to skin function; narrow skin barrier function usually Refers to the physical or mechanical barrier structure of the epidermis, especially the stratum corneum. Having a healthy skin barrier is tantamount to having beautiful, natural-looking skin. However, due to factors such as environment, diet and wrong skin care, the skin barrier is damaged, which makes the skin's own defense ability insufficient, and the skin is extremely sensitive and damaged. Used in cosmetics, oil can make the skin soft, lubricated and purify the surface. It has a friction effect on the skin surface, and at the same time forms a hydrophobic film to protect it and prevent the invasion of harmful substances from the outside. It can also inhibit the moisture of the skin epidermis when it is cold. Evaporation protects skin and hair, leaving hair shiny and soft.

Microbial oil is another new edible oil resource developed by humans after vegetable oil and animal oil. Microorganisms capable of producing oil include yeast, mold, bacteria, and algae. Among them, eukaryotic yeast, mold, and algae can synthesize triglycerides similar to vegetable oil, while prokaryotic bacteria can synthesize special lipids. Microalgae contains essential nutrients such as protein, fat, carbohydrates and vitamins, etc., and the content of oil is relatively high. The content of neutral fat accounts for about 20-50% of the dry weight of cells, and a few microalgae can reach 75%. The amount of oil is significantly higher than that of agricultural oil crops. Therefore, microalgae oil is considered to be the oil resource with the most development potential, and has received great attention internationally. Most microalgae are rich in long-chain polyunsaturated fatty acids. The common unsaturated fatty acids in nature are mainly divided into three categories: omega-3 series, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid Dienoic acid (DHA); omega-6 linoleic acid series and omega-9 oleic acid series. Polyunsaturated fatty acids can regulate blood lipids, enhance the body's immunity, nourish the brain and improve the symptoms of arthritis and relieve pain. For example, the literature Jiang Mulan, the research progress of oily microalgae, Chinese Journal of Oil Crops [J], 2010, 32 (4): 571-576 pointed out that omega-3 series fatty acids are beneficial to the health of brain and retina, skin and kidney function. It is very important. DHA is called melatonin. It has important functions for the nervous system. It has the functions of strengthening the brain, improving memory and vision. It can especially promote the development of fetal brain cells and the growth of infant brain cells, and promote the improvement of memory in teenagers. Prevention and treatment of senile dementia. For example, the literature Liu Jihong, Significance of ω-3 polyunsaturated fatty acids in tumor prevention and treatment, parenteral and enteral nutrition [J], 2004, 11 (1): 54-56 pointed out that polyunsaturated fatty acids have better anticancer The role of postmenopausal breast cancer patients, the intake of EPA and DHA in the diet and the ratio of DHA to phospholipids in mammary adipose tissue are significantly lower than those of benign breast disease patients, so intake of omega-3 fatty acids can prevent the occurrence of breast cancer .

According to the source and chemical composition of oils, oils can be mainly divided into: vegetable oils, animal oils, mineral oils and synthetic oils. Vegetable oils are skin-friendly, close to the structure of human sebum membrane, with good permeability, deep penetration and nourishment. The skin is favored by many skin care products. Vegetable oil is a natural compound formed by the reaction of higher fatty acids and glycerol, which is widely distributed in nature. All fats extracted from plant seeds, pulp and other parts are collectively referred to as vegetable oils. Vegetable oils used in cosmetics mainly come from plant fruits, seeds and germs, and some come from other parts of plants such as leaves, bark, roots, petals and stamens. Common vegetable oils include avocado (Butyrospermum parkii) fruit fat (also known as shea butter), olive (Olea europaea) fruit oil (also known as olive oil), apricot (Prunus armeniaca) kernel oil (also known as sweet almond oil), Avocado (Persea gratissima) fruit oil, jojoba (Simmondsia chinensis) seed oil, safflower (Carthamus tinctorius) seed oil, white pond flower (Limnanthes alba) seed oil, sunflower (Helianthus annuus) seed oil, etc. Vegetable oil has a skin-friendly texture and is close to the human sebum membrane structure. It is easier to be absorbed by the skin than water and has better permeability, so it can penetrate deeply and nourish the skin.

The skin barrier generally refers to the physical barrier of the skin, including the stratum corneum of the skin epidermis and the sebum film on the surface of the stratum corneum. The stratum corneum is the outermost layer of the epidermis and has a "brick-wall structure", where "bricks" refer to corneocytes and "mortar" refers to intercellular lipids. In the process of skin barrier formation, more than 200 genes are highly related to skin barrier. For example, the barrier factors filaggrin (FLG), loricrin (LOR), endothelin (IVL), transglutaminase 1 (TGM1), and barrier moisturizing factor caspase 14 are related to the formation of the corneous envelope. (CASP14), HMGCoA reductase (HMGCR), aquaporin 3 (AQP3) and keratinocyte intercellular junction-associated factor zona occlusin 1 (ZO-1).

Specifically, filaggrin (FLG) is an important constituent protein of the cornified envelope of the outer layer of the skin epidermis, mainly present in the granular layer and transparent layer of the epidermis, and is an important component of the skin barrier; Hydrophilic free amino acids and water-absorbing derivatives help the skin store moisture, maintain skin moisture, and have moisturizing effects. Loricrin (LOR) is the main component of the corneous envelope and plays an important role in the barrier function of the epidermis. Indothelin (IVL) is mainly expressed in the upper part of the spinous cell layer and the granular layer. It is a marker protein for keratinocyte differentiation. Lipid matrix and keratinocytes are connected to function. The characteristic resistance and insolubility of the cornified envelope is based on the formation of very stable isopeptide bonds catalyzed by transglutaminase 1 (TGM1).

HMGCoA reductase (HMGCR) is the rate-limiting enzyme for synthesizing cholesterol. It exists in the endoplasmic reticulum and catalyzes the synthesis of methyldivalenic acid. Afterwards, it undergoes multi-step reactions such as decarboxylation and phosphorylation to generate squalene, and finally squalene is cyclized into wool. sterols are converted to cholesterol. Aquaporin 3 (AQP3) is the most abundant subtype of aquaporin in the AQPs family. Its expression in the epidermis has a spatial hierarchy, mainly expressed in the basal layer of the human epidermis, the spinous cell layer, and the granular layer, and gradually disappears in the stratum corneum . This spatial distribution is consistent with the water content distribution of the skin: the basal and suprabasal layers are about 75% water, while the stratum corneum is only about 10%-15%. The high expression of AQP3 in the basement membrane zone can promote the transport of water, glycerol and urea, making the extracellular environment of the basal layer closer to a neutral equilibrium state; the closer to the stratum corneum, the lower the expression of AQP3, and the lower the water content. The decline is obvious, and the internal environment of the skin gradually becomes acidic. In addition, AQP3 is responsible for the important role of glycerol transport in the stratum corneum. AQP3 transports endogenous glycerol into the epidermis, making it directly or indirectly affect the moisturizing effect of the skin.

Tight junctions are an important part of the physical barrier, mostly appearing in the epidermis of the skin, located on the top side of the gap between adjacent cells. Tight junctions are composed of different types of transmembrane proteins as well as intracellular cytoplasmic proteins. Transmembrane proteins such as water gate protein 1 (CLDN1), occlusion protein (OCLN), etc.; cytoplasmic proteins such as zonular occlusion protein-1 (ZO-1) and so on. ZO-1 is a cytoplasmic protein that belongs to the family of membrane-linked guanylate kinase proteins. It gathers occludins and waterlock proteins through extracellular signal transduction pathways to form basic cell junction strips; ZO-1 is a protein in the skin barrier function. One of the most characterized cytoplasmic proteins.

Plant bionic lipid technology (Phyto Bionic Sebum, referred to as PBS technology): select a variety of natural vegetable oils, through scientific proportioning, to simulate the natural lipid components in the skin barrier structure, to supplement the essential unsaturated fatty acids in the stratum corneum of the skin, such as flax Acid, linoleic acid, etc., supplement the lipid components that the skin lacks, and are compatible with sebum, so as to quickly repair the skin barrier and restore the skin to a healthy, moist and shiny state.

Among them: vegetable oil mainly comes from including but not limited to plant fruits (for example: shea butter, olive oil, almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, orange fruit oil, bergamot fruit oil , citron fruit oil, tomato fruit oil, rosehip oil, pepper fruit oil, brazil nectarine fruit oil, flat leaf pimento fruit oil, anise fruit oil, mulberry fruit oil, juniper fruit oil, Capsicum fruit oil, satsuma fruit oil, star fruit oil, rosehip oil and coriander fruit oil, etc.), seeds (for example: jojoba seed oil, safflower seed oil, white pond flower seed oil, sunflower seed oil, Macadamia Seed Oil, Castor Seed Oil, Peony Seed Oil, Grape Seed Oil, Grapefruit Seed Oil, Apple Seed Oil, Pomegranate Seed Oil, Watermelon Seed Oil, Zucchini Seed Oil, Flax Seed Oil, Rapeseed Oil, Sesame Seed Oil , borage seed oil, Burr walnut seed oil, cottonseed oil, tea seed oil, millet seed oil, papaya seed oil, raspberry seed oil, baobab seed oil, calendula seed oil, oleifera Seed Oil, Moringa Seed Oil, Pecan Seed Oil, Perilla Seed Oil, Babassu Seed Oil, and White Lupine Seed Oil, etc.) and Germ (Examples: Rice Germ Oil, Wheat Germ Oil, Oat Germ Oil, and Corn Germ Oil Oil, etc.), and some are derived from other parts of plants such as leaves, bark, roots, petals and stamens.

The present invention unexpectedly finds that microbial oils (such as microalgae oil, etc.) and vegetable oils (such as jojoba oil, safflower seed oil, sunflower seed oil, etc.) have skin barrier-related effects, which can enhance the vitality of the skin barrier and promote skin barrier function. Expression of related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, the composition comprising microbial oil and vegetable oil can be used as an efficacy additive in medicines and external skin preparations.

Contents of the invention

In one aspect, the present invention provides an oil composition, the oil includes microbial oil and vegetable oil, wherein, based on the total weight of the composition, the composition comprises at least 0.5% by weight of oil. In a preferred embodiment, the weight ratio of microbial oil to vegetable oil in the oil composition of the present invention is 1:8 to 8:1.

In a preferred embodiment, the oil composition of the present invention also includes a natural surfactant selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, sugars, alkanes base polyglycosides or their combinations. In a preferred embodiment, the natural surfactant is hydrogenated lecithin.

In a preferred embodiment, the weight ratio of oil and natural surfactant in the oil composition of the present invention is 1:1 to 20:1. In a more preferred embodiment, the weight ratio of vegetable oil to natural surfactant in the oil composition of the present invention is 10:1 to 20:1.

In a preferred embodiment, the vegetable oil in the oil composition of the present invention is vegetable seed oil, vegetable fruit oil or a combination thereof. For example, the vegetable seed oil is selected from: jojoba seed oil, safflower seed oil, white pond flower seed oil, sunflower seed oil, macadamia nut seed oil, castor oil, peony seed oil, grape seed oil, grapefruit seed oil Oil, Apple Seed Oil, Pomegranate Seed Oil, Watermelon Seed Oil, Zucchini Seed Oil, Flax Seed Oil, Rapeseed Oil, Sesame Seed Oil, Borage Seed Oil, Burr Hard Walnut Seed Oil, Cottonseed Oil, Tea Seed Oil, Guava Seed Oil, Papaya Seed Oil, Raspberry Seed Oil, Baobab Seed Oil, Calendula Seed Oil, Oleifera Seed Oil, Moringa Seed Oil, American Pecan Seed Oil, Perilla Seed Oil, Babassu seed oil, white lupine seed oil, or a combination thereof. For example, the plant fruit oil is selected from: shea butter, olive oil, sweet almond oil, avocado fruit oil, sea buckthorn fruit oil, coconut fruit oil, orange fruit oil, bergamot fruit oil, citron fruit oil , tomato fruit oil, dog tooth rosehip oil, pepper fruit oil, Brazilian nectarine fruit oil, flat-leaf pimento fruit oil, anise fruit oil, mulberry fruit oil, juniper fruit oil, pheasant fruit oil, Satsuma fruit oil, star palm fruit oil, rosehip oil, coriander fruit oil or a combination thereof.

In a preferred embodiment, the microbial oil in the oil composition of the present invention is selected from: oil of microalgae, oil of Haematococcus pluvialis, oil of Dentate algae or combinations thereof.

On the other hand, the present invention also relates to the application of the oil composition in enhancing skin barrier function. In a preferred embodiment, the enhancement of the skin barrier function is achieved by enhancing the vitality of the skin barrier and/or promoting the expression of skin barrier-related factors.

On the other hand, the present invention also relates to the application of the oil composition in the preparation of medicines and/or skin external preparations for enhancing skin barrier function. In a preferred embodiment, the pharmaceutical and/or external skin preparation contains 0.001-100% by weight of the composition.

Detailed ways

The present invention relates to a composition comprising microbial oil and vegetable oil and its application. It has been found in the research that the composition has the ability to enhance the vitality of the skin barrier and promote the skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and Effect of ZO-1 Expression. Therefore, the composition comprising microbial oil and vegetable oil can be used as an efficacy additive for the preparation of medicines and/or skin external preparations for enhancing the skin barrier function.

In order to provide a more concise description, some quantitative expressions given herein are not modified by the term "about". It is to be understood that, whether or not the term "about" is explicitly used, each quantity given herein is intended to refer to the actual given value, and also to refer to what one of ordinary skill in the art could reasonably infer. Approximations to these given values include approximations to these given values that result from experimental and/or measurement conditions.

In order to provide a more concise description, some quantitative expressions are described herein as ranging from about X amount to about Y amount. It should be understood that when a range is recited, that range is not limited to the upper and lower limits recited, but includes the entire range from about X amount to about Y amount or any amount therebetween.

microbial oil

The composition of the present invention comprises microbial oil selected from: microalgae oil, Haematococcus Pluvialis (HAEMATOCOCCUS PLUVIALIS) oil, long ear algae (ODONTELLA AURITA) oil. Microalgae oil is an additive in health products, medicine and cosmetics, and can also be used as a renewable energy source for edible oil. In terms of food, microalgae oil can be used as a functional nutritional enhancer or directly made into health care products. Microalgae oil not only contains unsaturated fatty acids such as DHA and EPA, but also contains hydrocarbons and trace alcohols that are beneficial to human health . In terms of medicine, functional polyunsaturated fatty acids in microalgae oil can be used as medicine. At present, linoleic acid and arachidonic acid in microalgae oil are widely used in the medical field. Congestion and digestive system ulcers and other diseases are very effective. In the farming industry, microalgae oil can be added to the feed to obtain the functional agricultural and sideline products we want.

The microalgae oil in the present invention is glyceryl trioleate, which is rich in more than 90% of omega-9 unsaturated fatty acid beneficial to human body, ie oleic acid. Oleic acid is a monounsaturated fatty acid commonly found in the diet and is not an essential fatty acid, but it still has benefits for general health. Omega-9s are found in olives, almonds, queensland nuts, hazelnuts, sesame, avocado, flaxseed and their oils. High consumption of olive oil in Mediterranean countries has been linked to lower rates of breast cancer and heart disease. Research from the University of California pointed out that a diet high in monounsaturated fatty acids can prevent the oxidation of low-density lipoprotein cholesterol and protect blood vessel walls. This type of fat can also promote the function of high-density cholesterol to control low-density cholesterol. Saturated fat makes the antioxidant vitamin E more effective in protecting cells from free radical damage.

The present invention surprisingly found that compositions comprising microbial oils (e.g., microalgae oil) have skin barrier-related effects, can enhance skin barrier vitality and promote skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and Expression of ZO-1.

In some embodiments, the composition of the present invention comprises 0.1-100% by weight of microbial oil. In a preferred embodiment, the composition of the present invention comprises 0.1-50% by weight of microbial oil. In a preferred embodiment, the composition of the present invention comprises 0.1-20% by weight of microbial oil. In a preferred embodiment, the composition of the present invention comprises 0.5-10% by weight of microbial oil.

vegetable oil

Vegetable oil is widely used in food, industry, cosmetics and medicine. The most widely used vegetable oil in cosmetics is shea butter. Shea butter has many effects and functions, such as excellent nourishing and moisturizing functions. This is because Shea butter is rich in unsaponifiable substances, contains a lot of fat and fatty acids, etc., and has a strong moisturizing function. In addition, high-quality shea butter has good UV protection because it contains an excellent natural sunscreen ingredient-cinnamic acid, which can absorb ultraviolet rays in the sun and protect the skin from sun damage; moreover, high-quality milk Argan oil also has very effective anti-inflammatory and anti-allergic functions, so it is suitable for relatively weak and sensitive parts such as eyes and lips, and its phytosterol component has a good anti-inflammatory and repairing effect on injured skin.

The compositions of the present invention may comprise vegetable fats and oils from various sources. For example, compositions of the invention comprise vegetable fruit oils. Vegetable fruit oil, also known as plant fruit oil, mainly comes from plant fruits, seeds and germs, and some come from other parts of plants such as leaves, bark, roots, petals and stamens. Plant fruit oils include, but are not limited to, avocado (Butyrospermum parkii) fruit butter (also known as shea butter), olive (Olea europaea) fruit oil (also known as olive oil), apricot (Prunus armeniaca) kernel oil (also known as sweet Almond Oil), Avocado (Persea gratissima) Fruit Oil, Sea Buckthorn (Hippophae rhamnoides) Fruit Oil, Coconut (Cocos nucifera) Fruit Oil, Orange (Citrus junos) Fruit Oil, Bergamot (Citrus aurantium bergamia) Fruit Oil, Citron (Citrus bedica limonum) Fruit Oil, Tomato (Solanum lycopersicum) Fruit Oil, Rosa canina (Rosa canina) Fruit Oil, Pepper (Piper nigrum) Fruit Oil, Brazilian Nectarine (Caryocar brasiliense) Fruit Oil, Flat Leaf Pansy ( Vanilla planifolia) fruit oil, Pimpinella anisum fruit oil, Mauritia flexuosa fruit oil, Juniperus communis fruit oil, Litsea cubeba fruit oil, Citrus unshiu fruit oil Oil, Astrocaryum vulgare, Rosa multiflora, Coriandrum sativum, etc.

For example, shea butter is relatively close to the indicators of human sebum secretion and oil. It is rich in non-saponifiable ingredients, easy to absorb by the human body, can prevent dryness and cracking, further restore and maintain the natural elasticity of the skin, protect the skin barrier function, and at the same time Can play an anti-inflammatory effect. Olive oil has a slightly heavy skin feel and is very suitable for dry skin. The squalene and fatty acids contained in it can be quickly absorbed by the skin, effectively keeping the skin elastic and moisturized. It is rich in monounsaturated fatty acids, vitamins, and phenols The substance can eliminate facial wrinkles, prevent skin aging, have the effects of skin care and hair care, and prevent chapped hands and feet. Sweet Almond Oil softens, conditions, and is great for repairing and moisturizing dry, cracked, itchy or irritated skin, especially for dry and sensitive skin. Avocado oil is used as a lubricant for cosmetics, which can make the skin smooth, has good permeability and moisturizing properties, and can promote skin cell regeneration. It has a good effect on rebuilding skin structure, repairing sunburn and dry skin. Coconut oil is a saturated fat, stable lipid, not easy to oxidize and produce free radical attack, its strong antioxidant capacity can help the human body prevent the generation of free radicals, so it can be used for beauty and skin care, its emulsification stability and antioxidant properties can make Makeup is more even and delicate.

The present invention unexpectedly finds that compositions comprising vegetable oils (for example, shea butter) have skin barrier-related effects, can enhance skin barrier vitality and promote skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 expression.

In some embodiments, the composition of the present invention comprises 0.1-100% by weight of vegetable oil. In a preferred embodiment, the composition of the invention comprises 0.1-50% by weight of vegetable oil. In a preferred embodiment, the composition of the invention comprises 0.1-20% by weight of vegetable oil. In a preferred embodiment, the composition of the invention comprises 0.5-10% by weight of vegetable oil.

Compositions comprising microbial oils and vegetable oils

In some embodiments of the present invention, a composition comprising microbial oil and vegetable oil is provided, wherein the weight ratio of the microbial oil to the vegetable oil is 1:8 to 8:1. In a preferred embodiment, the weight ratio of the microbial oil to the vegetable oil is 1:1.

In some embodiments, the composition of the present invention comprises 0.1-100% by weight of microbial oil and vegetable oil. In a preferred embodiment, the composition of the present invention comprises 0.1-50% by weight of microbial fats and vegetable fats. In a preferred embodiment, the composition of the invention comprises 0.1-20% by weight of microbial fats and vegetable fats. In a preferred embodiment, the composition of the present invention comprises 0.5-10% by weight of microbial fats and vegetable fats.

natural surfactant

The compositions of the present invention may also contain natural surfactants. Natural surfactants mostly come from plants and animals, which are relatively complex polymer organic matter. Due to its strong hydrophilicity, it can form emulsions. Most of these substances have higher viscosity, which is beneficial to emulsification stability.

The natural surfactant contained in the composition of the present invention includes but not limited to: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, sugars, alkyl polyglycosides and the like. Lecithin exists in biological cells, such as animal eggs, brains and other tissues and plant seeds or germs, and egg yolk phosphatidylcholine is extracted from egg yolk; soybeans are rich in lecithin. Lecithin has physiological activities such as emulsification, dispersion, and anti-oxidation. It is a natural and excellent surfactant and an important emulsifier.

Hydrogenated lecithin has strong hydrophilicity and moisturizing properties, and has a strong affinity for skin and mucous membranes. It can be used in cosmetic formulations to moisturize, emulsify and disperse; as a surfactant, it can also condition the skin , to achieve a good oil-water balance effect, the use of hydrogenated lecithin can also develop skin care cream, hand cream, lipstick, sunscreen and other advanced cosmetic products.

In some embodiments, the compositions of the present invention comprise 0.1-20% by weight of natural surfactants. In a preferred embodiment, the compositions of the invention comprise 0.1-10% by weight of natural surfactants. In a preferred embodiment, the compositions of the invention comprise 0.1 to 5% by weight of natural surfactants. In a preferred embodiment, the compositions of the invention comprise 0.5-2% by weight of natural surfactants.

In a preferred embodiment, the weight ratio of oil (including microbial oil and vegetable oil) to natural surfactant in the composition of the present invention is 1:1 to 20:1. In a more preferred embodiment, the weight ratio of the oil in the composition (including microbial oil and vegetable oil) to the natural surfactant is 10:1 to 20:1.

Medicines and/or skin topical agents

The composition containing microbial oil and vegetable oil can be applied to pharmaceuticals and/or skin external preparations for enhancing skin barrier function.

In some embodiments, the drug is selected from: tablets, capsules, emulsions, suspensions, powders, granules, solutions, and various drug dosage forms known in the art. Add different dosages according to different types of dosage forms.

In some embodiments, the external skin preparation is selected from: cleanser, lotion, lotion, cream, gel, mask. Add different dosages according to different types of preparations.

The skin external preparation is a general term for all ingredients that are usually used on the outside of the skin, and may be, for example, a cosmetic composition. The cosmetic composition can be basic cosmetics, facial makeup cosmetics, body cosmetics, hair care cosmetics, etc. There is no special limitation on the dosage form, which can be reasonably selected according to different purposes. The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different dosage forms and purposes.

External preparations for skin contain a dermatologically acceptable carrier or vehicle (eg, lotion, cream, ointment, cleanser, etc.). Those of ordinary skill in the art can select a carrier capable of dissolving or dispersing these components at the above-mentioned concentrations according to common knowledge in the art.

Those of ordinary skill in the art can select suitable carriers based on common knowledge and their ability to dissolve or disperse in the active ingredient at the concentration most suitable for processing the active ingredient. Said carrier includes, for example, water, alcohols, oils, etc. wait.

The external preparation for skin of the present invention may be in the form of a topical product which can be externally applied to the skin and which can be prepared by those ordinary techniques known in the art. The carrier may take various practical forms, such as creams, dressings, gels, lotions, ointments or liquids, including compositions for application and for rinsing off, and their addition to, for example, dry or Wet application, hydrogel matrix, or adhesive (or non-adhesive) patch material carrier. Preferably, the carrier is a gel or moisturizing lotion, or an applicator in dry or wet form.

Typical carriers include emulsions containing water and/or alcohol and emollients such as oils and waxes of hydrocarbons, silicone oils, hyaluronic acid, vegetable, animal or marine fats or oils, glyceride derivatives, Fatty acids, or fatty acid esters or alcohols or alcohol ethers, lanolin and its derivatives, polyols or esters, wax esters, sterols, phospholipids, etc., and generally emulsifiers (nonionic, cationic or anionic), Although some emollients have inherent emulsifying properties. Additionally, these same components can be formulated into creams, gels, or solid sticks using different proportions of their components and/or by incorporating thickeners such as gums or other forms of hydrocolloids.

The skin external preparation of the present invention may contain additional components commonly found in skin care compositions, such as emollients, skin conditioners, emulsifiers, preservatives, antioxidants, fragrances, chelating agents, etc., as long as they are compatible with the skin Other components in the external preparation are physically and chemically compatible and do not affect the effect of the microbial oil and vegetable oil of the present invention.

In some embodiments of the external preparation for skin of the present invention, one or more preservatives may be used. Suitable preservatives include p-hydroxyacetophenone, C1-C4 alkylparabens and phenoxyethanol. Preservatives are used in an amount of about 0.5 to about 2% by weight, preferably about 0.5 to 1% by weight, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more antioxidants can be used. Suitable antioxidants include butylated hydroxytoluene (BHT), ascorbyl palmitate (BHA), butylated hydroxyanisole, phenyl-alpha-naphthylamine, hydroquinone, propyl gallate, nordihydroguaiaretic acid , vitamin E or vitamin E derivatives, vitamin C and its derivatives, calcium pantothenate, green tea extract and mixed polyphenols, and mixtures of the foregoing. The antioxidant is used in an amount ranging from about 0.02 to 0.5% by weight of the total composition, more preferably from about 0.002 to 0.1% by weight.

In one example of the skin external preparation of the present invention, one or more emollients may be used, which act as lubricants by their ability to remain on the skin surface or in the stratum corneum to reduce flaking, improve The appearance of the skin. Typical emollients include fatty esters, fatty alcohols, mineral oil, polyether siloxane copolymers, and the like. Examples of suitable emollients include, but are not limited to, Polypropylene Glycol ("PPG")-15 Stearyl Ether, PPG-10 Cetyl Ether, Steareth-10, Oleth-8, PPG-4 Lauryl Ether Cetyl Ether, Vitamin E Acetate, Lanolin, Cetyl Alcohol, Cetearyl Ethylhexanoate, Cetostearyl Alcohol, Glyceryl Stearate, Capryl Hydroxystearate, Dimethicone Alkanes, and combinations thereof. Cetyl alcohol, cetearyl ethylhexanoate, cetostearyl alcohol, glyceryl stearate and combinations thereof are preferred. When used, emollients are present in amounts ranging from about 0.1 to about 30%, preferably from about 1 to about 30% by weight, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more moisturizing agents can also be used. Humectants, also known as humectants, help to enhance the effectiveness of emollients, reduce flaking, stimulate removal of constituent scales and improve skin feel. Polyols may be used as humectants including, but not limited to, glycerin, polyalkylene glycols, alkylene polyols and their derivatives, including butylene glycol, propylene glycol, dipropylene glycol, polyglycerol, polyethylene glycol Alcohol and its derivatives, sorbitol, hydroxypropyl sorbitol, hexanediol, 1,3-dibutylene glycol, 1,2,6-hexanetriol, ethoxylated glycerin, propoxylated glycerin, and their combinations. When used, humectants are used in amounts of about 0.1 to about 20% by weight, preferably about 1 to about 15% by weight, based on the total weight of the composition.

In one example of the external preparation for skin of the present invention, one or more emulsifiers may be used. Emulsifiers can be used in effective stabilizing amounts. Preferably, emulsifiers are used in amounts of from about 1.0 to about 10.0 percent by weight, more preferably from about 3.0 to about 6.0 percent by weight, based on the total weight of the composition. Any emulsifier compatible with the ingredients of the composition may be used. Suitable emulsifiers include Stearic Acid, Cetyl Alcohol, Glyceryl Stearate, Lecithin, Stearyl Alcohol, Steareth-2, Steareth-20, Acrylates/C10-30 Alkyl Acrylates Crosslinked Polymers, and their combinations.

In one example of the external preparation for skin of the present invention, one or more pH adjusting agents can be used. Beneficial pH regulators in the external preparation for skin of the present invention include tromethamine. When used, the pH adjuster is used in an amount of about 0.1 to about 2% by weight, preferably about 0.1 to about 1% by weight, based on the total weight of the composition.

In a specific embodiment of the present invention, the skin external preparation comprises acrylic acid (ester) type/C10-30 alkanol acrylate crosslinked polymer, glycerin, p-hydroxyacetophenone, glyceryl stearate and lecithin, Hexetearyl Alcohol, Cetearyl Ethylhexanoate, Tromethamine, or combinations thereof.

In some embodiments of the present invention, the dosage of the composition of the present invention in external skin preparations is 0.001%-100% (w/w), preferably 0.01%-60% (w/w) by weight, more The preferred weight percentage is 0.01%-40% (w/w).

Example

Below in conjunction with specific embodiment, to further illustrate the present invention. It is necessary to point out here that embodiment is only used to further illustrate the present invention, can not be interpreted as the limitation of protection scope of the present invention, those skilled in the art can make some non-essential improvements according to the content of the present invention above and adjust. The test methods for which specific conditions are not indicated in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer. All percentages and parts are by weight unless otherwise indicated.

Experimental materials adopted in the following examples include:

Triolein, trade name microalgae oil, purchased from LUBRIZOL ADVANCED MATERIALS, INC.

Avocado tree (Butyrospermum parkii) fruit fat, trade name refined shea butter, purchased from BASF Advanced Materials Co., Ltd.;

Hydrogenated lecithin, trade name hydrogenated lecithin, purchased from NIKKO CHEMICALS CO.,LTD.

The experimental equipment adopted in the embodiment includes:

Weighing balance: METTLER TOLEDO company PB4002-N type;

pH meter: SEVENMULTI type from METTLER TOLEDO;

Water bath: Shanghai Yiheng Technology Co., Ltd. HWS28 electric constant temperature water bath;

Homogenizer: POLYTRON company PT 3100D type;

Stand mixer: IKA@EUROSTAR, power control-visc..

Embodiment 1: preparation composition

Weigh 0.5 parts by mass of hydrogenated lecithin into a beaker, add 99.5 parts by mass of deionized water, stir evenly, and set aside.

Embodiment 2: Preparation comprises the composition of vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, then weigh 94.5 parts by mass of deionized water at 75-80°C in the beaker, and turn on Homogenizing, the speed is 3000 rpm, homogenizing for 5-10 minutes, and set aside.

Embodiment 3: Preparation comprises the composition of microbial grease

Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of microalgae oil in a beaker, heat to 75-80°C while stirring, then weigh 94.5 parts by mass of deionized water at 75-80°C in the beaker, turn on the homogenizer Homogenize at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 4: Preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 1 part by mass of microalgae oil and 8 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 5: preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 3 parts by mass of microalgae oil and 6 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 6: Preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 4.5 parts by mass of microalgae oil and 4.5 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 7: Preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 6 parts by mass of microalgae oil and 3 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 8: Preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 7 parts by mass of microalgae oil and 2 parts by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Embodiment 9: Preparation comprises the composition of microbial oil and vegetable oil

Weigh 0.5 parts by mass of hydrogenated lecithin, 8 parts by mass of microalgae oil and 1 part by mass of shea butter in a beaker, heat to 75-80°C while stirring, and then weigh 90.5 parts by mass of 75-80°C detoxified Put the ionized water in the beaker, turn on the homogenizer, the speed is 3000 rpm, homogenize for 5-10 minutes, and set aside.

Test example 1: 3D recombinant skin epidermis model barrier-related gene expression test

The 3D epidermal reconstruction model is a human-derived 3D epidermal model that can simulate the structure of each layer of the epidermis differentiated by keratinocytes. Cover an appropriate amount of samples on the epidermal model for 24 hours, then collect the total ribonucleic acid (RNA) of the epidermal tissue, use RNA reverse transcription technology and gene microarray technology to quantitatively detect skin barrier-related genes, and then evaluate the skin barrier moisturizing and repair ability.

1. Test material

1.1 Cell: skin model is 3D epidermal skin model

(EpiSkin).

1.2 Reagents:

3D skin model maintenance medium (EpiSkin); DPBS containing calcium and magnesium (Gibco); isopropanol, 75% absolute ethanol (Sinopharm Group); DEPC water (Tiangen Biology); total RNA extraction reagent TRIzol (ThermoFisher); Reverse transcription kit iScript cDNA Synthesis Kit, fluorescence real-time quantitative PCR reagent iTaq ^TM Universal

Green Supermix (Bio-rad).

1.3 Main equipment

CO ₂ incubator HERAcell 240i, biological safety cabinet 1300SERIES A2, ultra-micro ultraviolet spectrophotometer NanoDrop ONE (Thermo); gene amplification instrument C1000Touch, real-time quantitative PCR instrument CFX Connect (Bio-rad); high-speed refrigerated centrifuge MIKRO 220R ( Hittich); positive pipette M25 (Gilson); 50 mL continuous pipette tip Multipette plus (Eppendorf); skin punch (area 0.38 cm ² ).

2. Experimental method:

3D reconstructed epidermis model reception and recovery

Receive the 3D epidermal remodeling model, check the integrity of the package, check the number of models, check the maintenance medium, and detect the color and volume of the medium. After confirming that it is correct, start model recovery.

Perform resuscitation operations in a biological safety cabinet. Take a certain number of 12-well plates, add 2 ml/well of maintenance medium to the first row, then take out the 3D recombinant epidermal model with tweezers, carefully remove the surface gel with a cotton swab, and put it into the well containing maintenance medium , recovery for 24 hours. One 12-well plate can recover 4 3D reconstructed epidermal models. Culture conditions: 5% CO ₂ , cultured in a constant temperature incubator at 37°C.

3D Reconstructed Epidermis Model Sample Processing

The model was divided into sample groups of Examples 1-9 (3 repetitions for each sample) and a positive control group (rosiglitazone, 3 repetitions).

First add 2 ml/well of maintenance medium to the second row of the 12-well plate, and then use tweezers to transfer the 3D recombinant epidermal model located in the first row to the corresponding second row.

Use a piston displacement pipette (M25) to add 15 microliters of rosiglitazone solution (100 micromoles, dissolved in 1×DPBS) to the positive control group, add 15 microliters of samples to the sample group, and Gently spread the sample evenly on the surface of the skin model, and each sample was repeated three times. All models were placed in a cell culture incubator and cultured for 24 hours. Culture conditions: 5% CO ₂ , cultured in a constant temperature incubator at 37°C.

3D Reconstituted Epidermis Model to Collect Total RNA Samples

Use a 50ml continuous pipette to absorb 1×DPBS, rinse off the sample on the surface of the model (do not flush directly at the sample, but aim at the side wall of the skin model support frame), and then use a cotton swab to absorb the residual liquid. Cut the epidermal tissue with a hole punch, and separate the epidermal tissue from the underlying collagen base with two small elbow tweezers, put the epidermal tissue into an EP tube containing 1 ml of total RNA extraction reagent (TRIzol), and use Pipette the epidermal tissue repeatedly with a 1 ml RNase-free pipette until only the transparent stratum corneum remains. Subsequently, 0.2 ml of chloroform was added to the original EP tube, shaken vigorously, and centrifuged at low temperature and high speed for 15 minutes. Use an RNase-free pipette nozzle to take 500 microliters of the upper aqueous phase, then add an equal volume of pre-cooled (minus 20 degrees) isopropanol, mix by inversion, and centrifuge at low temperature and high speed for 10 minutes. After centrifugation, the RNA forms a white pellet at the bottom of the EP tube. Rinse twice with 75% ethanol, remove residual ethanol with a centrifuge and a suction nozzle, and open the lid to dry at room temperature until it becomes transparent. Dissolve with 40 µl RNase-free H ₂ O and mix well. The RNA concentration was measured with a micro-spectrophotometer Nanodrop, and the integrity of the RNA was detected by agarose gel electrophoresis. The dissolved total RNA of the epidermis model tissue was used for subsequent experiments or stored at -80°C for long-term storage after aliquoting.

Quantitative detection of skin barrier-related genes by RT-qPCR

Take 1 microgram of total RNA for reverse transcription, and use the reverse transcription kit iScript cDNA Synthesis Kit to reverse transcribe to obtain cDNA.

The reverse transcription system is as follows:

The reverse transcription program was as follows: 25°C, 5 minutes; 42°C, 30 minutes; 85°C, 5 minutes.

Fluorescent real-time quantitative polymerase chain reaction (PCR) reagent iTaq ^TM Universal

Green Supermix performs fluorescence real-time quantitative detection to analyze the expression of barrier-related gene messenger RNA (mRNA).

The reaction system is as follows:

The reaction program is as follows: 95°C, 30 seconds; 95°C, 15 seconds, 60°C, 30 seconds, 40 cycles; 65-95°C, 0.5°C decrease each time (melting curve).

The primer sequences used in this example are designed as follows:

The 2 ^-ΔΔCt method was used to analyze the gene expression changes of the sample group of the example and the positive control group (rosiglitazone) relative to the control group of Example 1.

3. Result judgment

The amount of gene expression in the sample group in Example 1 is taken as 100%, and compared with it in each group, it is greater than 100%, indicating that the gene expression is up-regulated, and less than 100%, indicating that the gene expression is down-regulated. The up-regulation of genes such as FLG and LOR indicates that this sample group has the ability to moisturize and promote barrier repair; the down-regulation of genes such as FLG and LOR indicates that this sample group does not have the ability to moisturize and promote barrier repair.

Table 2: Expression of skin barrier factors in each group of samples after treatment

As shown in Table 2, the sample of Example 1 (microalgae oil 0%+shea butter 0%) is used as a control, and its gene relative expression is 100%. The gene expression of samples 8 and 9 and the positive control sample were compared to obtain the corresponding percentages.

Specifically, the barrier factor FLG in the sample of Example 2 (shea butter 5%), the sample of Example 3 (microalgae oil 5%), the sample of Example 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 222%, 340%, 381%, 439%, 767%, 457%, 381%, 340%, and 362%.

Specifically, the barrier factor LOR in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 240%, 319%, 422%, 504%, 839%, 544%, 422%, 323%, and 351%.

Specifically, the barrier factor IVL in Example 2 sample (shea butter 5%), embodiment 3 sample (microalgae oil 5%), embodiment 4 sample (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 113%, 75%, 116%, 132%, 120%, 129%, 116%, 113%, and 103%.

Specifically, the barrier factor TGM1 in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 134%, 134%, 178%, 211%, 246%, 195%, 178%, 149%, and 161%.

Specifically, the barrier factor CASP14 in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 188%, 316%, 300%, 485%, 667%, 424%, 300%, 279%, and 330%.

Specifically, the barrier factor HMGCR in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 170%, 281%, 291%, 326%, 450%, 342%, 291%, 284%, and 229%.

Specifically, the barrier factor AQP3 in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8% ), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6% + shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), embodiment 9 sample (microalgae oil 8%+shea butter 1%) and positive control ( Rosiglitazone) after treatment, compared with Example 1 sample (microalgae oil 0%+shea butter 0%), respectively 112%, 131%, 151%, 197%, 204%, 167%, 151%, 171%, and 131%.

Specifically, the barrier factor ZO-1 in the sample of embodiment 2 (shea butter 5%), the sample of embodiment 3 (microalgae oil 5%), the sample of embodiment 4 (microalgae oil 1%+shea butter 8%), Example 5 sample (microalgae oil 3%+shea butter 6%), embodiment 6 sample (microalgae oil 4.5%+shea butter 4.5%), embodiment 7 sample (microalgae oil 6%+shea butter 3%), Example 8 sample (microalgae oil 7%+shea butter 2%), example 9 sample (microalgae oil 8%+shea butter 1%) and positive After the control (rosiglitazone) was treated, it was 143%, 214%, 314%, 318%, 402%, 303%, respectively, compared with the sample of Example 1 (microalgae oil 0%+shea butter 0%) %, 314%, 240%, and 246%.

In summary, the sample in Example 2 (5% shea butter) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the function of promoting skin moisturizing and barrier repair. Protective effect. The sample of Example 3 (microalgae oil 5%) promotes the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the effect of promoting skin moisturizing and barrier repair. The sample of Example 4 (microalgae oil 1%+shea butter 8%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, is conducive to barrier formation, and has the function of promoting skin moisturizing and barrier repair. The sample of Example 5 (microalgae oil 3%+shea butter 6%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the function of promoting skin moisturizing and barrier repair. The sample of Example 6 (microalgae oil 4.5%+shea butter 4.5%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, is conducive to barrier formation, and has the function of promoting skin moisturizing and barrier repair. The sample of Example 7 (microalgae oil 6%+shea butter 3%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the function of promoting skin moisturizing and barrier repair. The sample of Example 8 (microalgae oil 7%+shea butter 2%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the function of promoting skin moisturizing and barrier repair. The sample of Example 9 (microalgae oil 8%+shea butter 1%) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is beneficial to barrier formation and has the function of promoting skin moisturizing and barrier repair.

Application example

The compositions comprising microbial oils and vegetable oils of the present invention can be used as intermediate raw materials or functional additives for the preparation of medicines or external skin preparations, and the external skin preparations are preferably cosmetic compositions, including but not limited to creams, Preparation of lotion, gel, lotion, essence, mask, eye cream, aerosol (cleaning foam), spray, shower gel, massage oil, facial cleanser and other dosage forms.

The weight percent of the composition comprising microbial oil and vegetable oil in the skin external preparation of the present invention is 0.001%-100% (w/w); the preferred weight percent is 0.01%-60% (w/w); more The preferred weight percentage is 0.01%-40% (w/w).

The following are specific application examples of the compositions containing microbial oils and vegetable oils obtained in Examples 3-6 and 8 in external skin preparations, as well as the formulations and preparation methods of these dosage forms. Specific application examples are as follows:

Application example 1: Preparation of face cream

Application Example 2: Preparation of Emulsion

Application example 3: Preparation of eye cream

Application Example 4: Preparation of facial mask

Application Example 5: Preparation of Essence

Application example 6: Examples 2-9 in the present invention can also be used alone as skin external preparations.

Claims (12)

1. An oil composition, the oil includes microbial oil and vegetable oil, wherein, based on the total weight of the composition, the composition contains at least 0.5% by weight of oil.
2. The composition according to claim 1, the weight ratio of the microbial oil to the vegetable oil is 1:8 to 8:1.
3. The composition according to claim 1, wherein the composition also comprises a natural surfactant selected from the group consisting of: lecithin, cholesterol, lanolin, tea saponin, protein, saponins, Sugars, alkyl polyglycosides or combinations thereof.
4. The composition of claim 2, wherein said natural surfactant is hydrogenated lecithin.
5. The composition according to claim 3 or 4, wherein the weight ratio of the oil to the natural surfactant is 1:1 to 20:1.
6. The composition according to claim 5, wherein the weight ratio of the vegetable oil to the natural surfactant is 10:1 to 20:1.
7. The composition according to claim 1, wherein the vegetable oil is plant seed oil, plant fruit oil or their combination, wherein the plant seed oil is selected from the group consisting of jojoba seed oil, safflower seed oil, white pool Flower Seed Oil, Sunflower Seed Oil, Macadamia Seed Oil, Castor Seed Oil, Peony Seed Oil, Grape Seed Oil, Grapefruit Seed Oil, Apple Seed Oil, Pomegranate Seed Oil, Watermelon Seed Oil, Zucchini Seed Oil, Flax Seed Oil , Rapeseed Oil, Sesame Seed Oil, Borage Seed Oil, Burr Hard Walnut Seed Oil, Grass Cotton Seed Oil, Tea Seed Oil, Millet Seed Oil, Papaya Seed Oil, Raspberry Seed Oil, Baobab Seed Oil, Gold Calendula seed oil, oleifera seed oil, Moringa seed oil, American hickory seed oil, perilla seed oil, babassu seed oil, white lupine seed oil or their combination, wherein the plant fruit oil is selected from From: Shea Butter, Olive Fruit Oil, Sweet Almond Oil, Avocado Fruit Oil, Sea Buckthorn Fruit Oil, Coconut Fruit Oil, Orange Fruit Oil, Bergamot Fruit Oil, Citron Fruit Oil, Tomato Fruit Oil, Rosa Dogtooth Fruit Oil, Pepper Fruit Oil, Brazil Nectarine Fruit Oil, Pimento Fruit Oil, Anise Fruit Oil, Mauritius Fruit Oil, Juniper Fruit Oil, Litsea Pepper Fruit Oil, Wenzhou Satsuma Fruit Oil, Star Fruit Palm Fruit oil, rosehip oil, coriander fruit oil, or a combination thereof.
8. The composition according to claim 1, wherein the microbial oil is selected from the group consisting of microalgae oil, Haematococcus pluvialis oil, Dentate algae oil or their combination.
9. Application of the composition as claimed in claim 1 in enhancing the skin barrier function.
10. The application according to claim 9, wherein said enhancement of skin barrier function is achieved by enhancing the vitality of the skin barrier and/or promoting the expression of skin barrier-related factors.
11. Application of the composition as claimed in claim 1 in the preparation of medicines and/or skin external preparations for enhancing skin barrier function.
12. The use according to claim 11, the medicine and/or skin external preparation comprises 0.001-100% by weight of the composition.