KR102032784B1 - Composition containing pineapple ceramide with high level of stability - Google Patents

Abstract

The present invention relates to a composition having excellent precipitation stability. The composition according to the present invention is ceramide; And even if it contains components such as collagen, hyaluronic acid, tube extract, etc. excellent precipitation stability and can be utilized as a food composition, preferably a beverage composition.

Description

Composition containing pineapple ceramide with high level of stability

The present invention relates to a composition having excellent precipitation stability.

Ceramide is a sphingolipid consisting of sphingosine and fatty acids, which accounts for more than 40% of the keratinocyte lipids that make up the stratum corneum of the skin, and is essential for the formation and function of the stratum corneum. Ceramides serve as lipid barriers to inhibit evaporation of water and maintain the structure of the stratum corneum.

In addition, it decomposes into phytosphingosine on the surface of the skin, acting as an antimicrobial barrier against harmful harmful microorganisms, and plays an important role in controlling inflammation and wound healing. Extraction and synthesis methods exist for the preparation of ceramides. The production of ceramide from nature can be extracted from animal origin, plant origin and mold, but the ceramide of animal origin is rapidly reduced in supply, and the amount of ceramide derived from plants and fungi is also known to be extremely small. Therefore, as a solution to this problem, a method of chemically synthesizing ceramide is being sought, and the major production method of ceramide currently on the market is also a synthesis method. Ceramide is widely used in cosmetics and foods as a representative beauty ingredient and has been reported to moisturize the skin.

Collagen is a major component of connective tissue and is mainly present in bones and skin, but is a kind of light protein distributed throughout the body such as joints, membranes of each organ, and hair. As a fibrous structural protein, the basic structural unit of collagen is tropocollagen, which has a triple helix structure composed of three polypeptides of about 100,000 molecular weight. Since constituent amino acids contain a lot of glycine and proline and other proteins contain relatively little hydroxyproline or hydroxylysine, these two amino acid contents are a measure of collagen content in the organ. Collagen is not degraded by proteolytic enzymes other than collagenase. When collagen is denatured by heat or chemical treatment, it becomes gelatin. In particular, type 2 collagen is a matrix that forms cartilage, and is mainly distributed in glass cartilage and vitreous bodies, and is reported to play an important role in overall joint health. Non-denatured type 2 collagen refers to type 2 collagen that is glycosylated and not denatured. Non-denatured type 2 collagen is often extracted from chicken breast cartilage and is reported to be useful for osteoarthritis and rheumatoid arthritis in very small amounts.

Cauliflower extract has long been used as a food ingredient in Europe and Asia, such as Garnish and Italian Sibrio, and it can replace the function of hyaluronic acid by containing various substances including natural hyaluronic acid. In addition, some broth extracts are generally recognized as safe by the FDA as GRAS (Generally Recognized As Safe).

As described above, in the case of the composition containing ceramide, which is beneficial to the human body, and added collagen and / or hyaluronic acid, there is a problem that precipitation problem occurs and the sensory characteristics are deteriorated.

Republic of Korea Patent Publication No. 10-2013-0125523

The problem to be solved by the present invention is to provide a composition, preferably a food composition, more preferably a beverage composition with improved sedimentation stability, including the ceramide and the ceramide and the components that can be precipitated together.

In order to solve the above problems, the present invention is ceramide; And collagen, hyaluronic acid and composition comprising any one or more selected from the group consisting of, extract, comprising a gellan gum improved stability, preferably a food composition, more preferably a beverage composition.

The present inventors have studied to improve the sedimentation stability of the composition containing the ceramide and the component included with the ceramide to cause precipitation, the result of the addition of gellan gum, preferably low acyl gellan gum It was confirmed that the precipitation stability was significantly improved and completed the present invention.

Hereinafter, the present invention will be described in detail.

In the present invention, the ceramide may be used without limitation to conventional materials known in the art, preferably glyceramide (glucoceramide) can be used.

The sugar ceramide may be a conventional substance known in the art without particular limitation as long as it is a substance having a structure in which sugar is bonded to ceramide. Examples include, but are not limited to, glucosyl ceramide, galactosyl ceramide, glucocerebroside, galactose cerboxide, lactosyl ceramide or mixtures of two or more thereof, and more preferably glucosyl ceramide. Can be used.

Most preferably, glucosyl ceramide derived from pineapple represented by the following formula (1) may be used among the glucosyl ceramides. The present inventors experimentally confirmed that the precipitation stability was improved by preparing a beverage composition together with a substance in which precipitation occurs using glucosyl ceramide derived from pineapple represented by the following Chemical Formula 1 among sugar ceramides.

That is, the ceramide in the present invention may be a ceramide derived from pineapple.

[Formula 1]

The ceramide may be included in 0.000001 to 5% by weight relative to the total weight of the composition, preferably 0.00001 to 3% by weight, and more preferably 0.0001 to 1% by weight.

The present invention includes, but is not limited to, a substance that causes precipitation with the ceramide, including collagen, hyaluronic acid, and / or broth extract. When the above-mentioned material is further included in the composition containing ceramide, precipitation is induced and the product value decreases because the precipitate is visible to the naked eye, and there is a problem that the sensory deterioration is caused by precipitation, but the present invention solves such a problem. It provides a composition that improves precipitation stability but does not affect organoleptic properties.

In the present invention, the collagen is not limited to anything as long as it is commonly used in the art, but is not limited thereto, and mammals such as fish, cattle, pigs, horses and sheep such as cod, carp, shark, flounder and sea bass, etc. It may be collagen extracted from algae, such as chicken, goose, turkey and duck.

The collagen may be included in an amount of 0.1 to 20% by weight, preferably 0.5 to 15% by weight, and more preferably 0.5 to 10% by weight, based on the total weight of the composition.

In the present invention, the hyaluronic acid is a substance having a repetitive structure of disaccharides in which N-acetyl D glucosamine (GlcNAc) and D-glucuronic acid (GlcA) are connected through β1-4 glycoside bonds, and the disaccharide is β1-3. The bond forms a hyaluronic acid. Hyaluronic acid is present in, but not limited to, almost all tissues of vertebrates, such as placenta, synovial fluid, free fluids, chicken clams, etc., and is present in large amounts in connective tissue areas, ie skin, synovial fluid, and the like.

The hyaluronic acid may be included in 0.00001 to 1% by weight relative to the total weight of the composition, preferably 0.00005 to 0.5% by weight may be included, more preferably 0.0001 to 0.1% by weight.

In the present invention, the cassia extract, that is, chicken rice extract refers to the extract extracted from chicken rice shackle, the chicken rice shackle can be used without being limited to the chicken rice shackle of any kind of chicken. The tube extract may be prepared by a conventional extraction method in the art, for example, may be extracted by treating the hydrolase. The tube extract may include 0.1 to 15% by weight of hyaluronic acid, but is not limited thereto.

The tube extract may be included in an amount of 0.01 to 10% by weight, preferably 0.05 to 5% by weight, and more preferably 0.1 to 3% by weight, based on the total weight of the composition.

In the present invention, the gellan gum is not limited thereto, but the microorganisms in the carbohydrate (for example, Pseudomonas elodea ), a heteropolysaccharide consisting of 1: 1: 2 of rhamnose, glucuronic acid and glucose. Microorganisms can be prepared by direct culturing, and commercially available gellan gum can be used.

Preferably, among the gellan gums, the present inventors have experimented to improve the precipitation stability in a composition containing a component precipitated together with ceramide and ceramide by using a low acyl gellan gum represented by Formula 2 below. Confirmed.

[Formula 2]

Here, n is 50 to 2000, preferably 50 to 1500, more preferably 100 to 1000.

Preferably, the low acyl gellan gum may have a molecular weight of 1 × 10 ⁵ to 7 × 10 ⁵ daltons, more preferably 1 × 10 ⁵ to 5 × 10 ⁵ daltons.

In other words, the present inventors are ceramide; And the addition of gellan gum, preferably low acyl gellan gum, to a composition comprising a component precipitated with ceramide including any one or more selected from the group consisting of collagen, hyaluronic acid, and broth extract. It was confirmed experimentally that the precipitation stability was remarkably improved without giving.

The gellan gum may be included in an amount of 0.0001 to 1% by weight, preferably 0.0005 to 1% by weight, and more preferably 0.001 to 0.5% by weight, based on the total weight of the composition.

In one aspect of the invention, the present invention is a food composition comprising any one or more selected from the group consisting of collagen, hyaluronic acid and cayenne extract, food composition, preferably a beverage composition with improved sedimentation stability including gellan gum To provide.

The food of the present invention may be prepared by a method commonly used in the art, and may be prepared by adding raw materials and ingredients commonly added in the art.

For example, the composition may include additional ingredients that are commonly used in food compositions to enhance odor, taste, visual appearance, and the like. For example, sugars, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, pantothenic acid, and the like can be included. . The sugars include monosaccharides, disaccharides, polysaccharides, and the like, and include, for example, glucose, lactose, trehalose, xylitol, and the like. Also, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), and copper (Cu) may be included. It may also contain amino acids such as lysine, tryptophan, cysteine, valine and the like. Also preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetic acid, etc.), fungicides (bleaching powder and highly bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisol (BHA), butylhydroxytoluene ( BHT), etc.), colorants (such as tar pigments), colorants (such as sodium nitrite, sodium nitrite), bleach (sodium sulfite), seasonings (such as MSG (sodium glutamate)), sweeteners (ducin, cyclate, saccharin, sodium Etc.), fragrances (fruit flavors, vanillin, lactones, etc.), swelling agents (alum, D-potassium hydrogen titanate, etc.), reinforcing agents, emulsifiers, thickeners (foils), coating agents, gum herbicides, foam inhibitors, solvents, modifiers, etc. Food additives may be added. The additive is selected according to the type of food and used in an appropriate amount.

In addition, a residual amount of water is included.

The composition according to the present invention is ceramide; And even if it contains components such as collagen, hyaluronic acid, tube extract, etc. excellent precipitation stability and can be utilized as a food composition, preferably a beverage composition.

Hereinafter, examples and the like will be described in detail to help understand the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example  Preparation of 1-3: preparation of beverage containing ceramide

After heating purified water, low acyl gellan gum (low acyl gellan gum) was added and dissolved in 0.005, 0.01 and 0.1% by weight, respectively, and used as stabilizers of Examples 1 to 3. When completely dissolved and the solution became transparent, pineapple extract and collagen, broth extract, sugar, and flavoring were added and dissolved in the composition shown in Table 1 below. When the formulation is completed, the homogeneous, sterilized process was put in a glass bottle to prepare a beverage. The gellan gum used in the manufacture was purchased and used in the market. Ceramide was used as the pineapple ceramide emulsion of Hwansun Pharmaceutical Co., Ltd., collagen and broth extracts were used as API raw materials, and sugars and spices were distributed at the market. The product was purchased and used.

Comparative example  Preparation of 1 to 5: Preparation of ceramide containing beverage

Comparative Examples 1 to 5 were prepared in the same manner as in Examples 1 to 3, but by changing the composition of the stabilizer. Stabilizers were prepared using high acyl gellan gum, xanthan gum, pectin, soybean polysaccharide, which are representative stabilizers used in beverages. The composition of Comparative Examples including Examples 1 to 3 is shown in Table 1. Stabilizers used in the manufacture were purchased from a commercially available stabilizer.

unit
(weight%) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 stabilizator low
Acyl
Gellan gum 0.005 0.01 0.1 - - - - - Xanthan Gum - - - - 0.03 - - - pectin - - - - - 0.3 - - Big head
Polysaccharides - - - - - - 0.3 - Hi
Acyl
Gellan gum - - - - - - - 0.01 Add
Raw material pineapple
Ceramide 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Collagen 10 10 10 10 10 10 10 10 Official
extract One One One One One One One One Trehalose 5 5 5 5 5 5 5 5 Peach flavor 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Purified water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100

Experimental Example  1: Confirmation of precipitation stability

The present inventors prepared Examples 1 to 3 and Comparative Examples 1 to 5 in order to confirm the precipitation stability of the beverage composition according to the present invention was observed for 4 months at 40 ℃, 75% humidity conditions to confirm the precipitation stability and the results It is shown in Table 2 below.

Precipitation 1 day past 10 days past 1 month 4 months Example 1 X X X X Example 2 X X X X Example 3 X X X X Comparative Example 1 O O O O Comparative Example 2 X O O O Comparative Example 3 O O O O Comparative Example 4 X O O O Comparative Example 5 X O O O

As a result, as shown in Table 2, precipitation was observed in other comparative groups except Examples 1 to 3, and in particular, all the comparative examples showed precipitation within 10 days. Examples 1 to 3 confirmed that no precipitation occurs for 4 months even under severe conditions, thereby maintaining stable properties.

Experimental Example  2: sensory evaluation

The present inventors proceeded to the sensory evaluation of the beverage prepared in Examples 1 to 3 and Comparative Examples 1 to 5.

The experiment is shown in Table 3 to the value of the body, aroma, and taste of 20 people who have 20 to 40 beverage type health functional food intake experience living in Daejeon area. The sensory evaluation was performed by dividing into Examples 1 to 3 and Comparative Examples 1 to 5 after 10 days of manufacture were shaken and ingested as they were.

In the above evaluation, 5 points were considered to be the best, 4 points were considered to be a little good, 3 points were moderate, 2 points were unsatisfactory, and 1 point was very unsatisfactory.

Body incense flavor Comprehensive Evaluation Shake before intake Example 1 4.4 4.0 4.0 4.1 Example 2 4.3 3.9 4.0 4.1 Example 3 4.7 3.7 3.8 4.1 Comparative Example 1 2.6 4.0 3.2 3.3 Comparative Example 2 4.4 4.0 3.8 4.1 Comparative Example 3 4.0 4.0 3.8 3.9 Comparative Example 4 4.1 3.8 3.9 3.9 Comparative Example 5 4.3 4.1 4.1 4.2 Intake Example 1 4.4 4.0 4.0 4.1 Example 2 4.3 3.9 4.0 4.1 Example 3 4.7 3.7 3.8 4.1 Comparative Example 1 2.6 4.0 3.2 3.3 Comparative Example 2 2.3 3.8 3.3 3.1 Comparative Example 3 2.5 3.8 3.1 3.1 Comparative Example 4 2.8 4.0 3.3 3.4 Comparative Example 5 2.7 3.9 3.1 3.2

As a result, in the case of Comparative Examples 1 to 5 in which precipitation occurred as shown in Table 3, the overall sensory performance including body feeling was significantly decreased when ingested as it is, but in Examples 1 to 3, precipitation did not occur according to the intake method. There was no difference in sensuality.

Example  Preparation of 4 to 6: Preparation of Ceramide-Containing Beverage

After heating purified water, 0.01% by weight of low acyl gellan gum was added to dissolve and used as a stabilizer of Examples 4 to 6. When the solution is completely dissolved and the solution becomes transparent, Examples 4 to 6 including ceramide must be included in order to identify a substance causing precipitation, and collagen and broth extracts are not included, and ceramide, collagen and broth extract are combined. The composition of the beverage containing all of the above was referred to Example 2. Sugars and fragrances that were completely dissolved without affecting the precipitation were combined together, and when the mixing was completed, the beverage was prepared in a glass bottle through a homogeneous and sterilization process. The gellan gum and xanthan gum used in the production were purchased from commercially available products, and pineapple ceramide was used as the pineapple ceramide emulsion of Hwansun Pharmaceutical Co., Ltd. Purchased and used a commercially available product.

Comparative example  Preparation of 6-9: Preparation of Ceramide-Containing Beverage

The present inventors prepared a beverage in the same manner as in Examples 4 to 6, but prepared a comparative example except for gellan gum and xanthan gum in order to determine the role of the precipitation inhibitor and the composition thereof is shown in Table 4 below.

unit
(weight%) Example 4 Example 5 Example 6 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 stabilizator Low acyl
Gellan gum 0.01 0.01 0.01 - - - - Add
Raw material pineapple
Ceramide 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Collagen - 10 - - 10 10 Official
extract - - One - - One One Trehalose 5 5 5 5 5 5 5 Peach flavor 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Purified water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100

Experimental Example  3: Check the precipitation stability

The inventors prepared Examples 4 to 6 and Comparative Examples 6 to 9 to observe precipitation stability at 40 ° C. and 75% humidity for 4 months to confirm precipitation stability, and the results are shown in Table 5 below.

division Precipitation Example 4 X Example 5 X Example 6 X Comparative Example 6 X Comparative Example 7 O Comparative Example 8 O Comparative Example 9 O

As shown in Table 5, in Comparative groups except for Examples 4 to 6 and Comparative Example 6, precipitation appeared and it was confirmed that there was a problem in stability. In other words, pineapple ceramide alone is not a problem when included in the beverage, but when contained together with collagen or cabbage extract is precipitated, it was confirmed that it can be suppressed by low acyl gellan gum.

Claims (7)

Ceramides; And
In the food composition comprising any one or more selected from the group consisting of collagen, hyaluronic acid and cayenne extract,
Food composition improved sedimentation stability, characterized in that it comprises a low acyl gellan gum (low acyl gellan gum) represented by the formula (2):
[Formula 2]

Here, n is 50 to 2000. The method of claim 1,
The ceramide is a food composition, characterized in that the sugar ceramide. The method of claim 1,
The ceramide is a food composition, characterized in that the ceramide derived from pineapple. The method of claim 1,
The ceramide is a food composition, characterized in that 0.000001 to 5% by weight based on the total weight of the composition. delete The method of claim 1,
The gellan gum is a food composition, characterized in that 0.0001 to 1% by weight based on the total weight of the composition. The method according to any one of claims 1 to 4, and 6,
The food composition is characterized in that the beverage.