KR102018240B1 - (r)-레티큘린 및 그 전구체를 제조하기 위한 조성물 및 방법 - Google Patents

(r)-레티큘린 및 그 전구체를 제조하기 위한 조성물 및 방법 Download PDF

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KR102018240B1
KR102018240B1 KR1020167017344A KR20167017344A KR102018240B1 KR 102018240 B1 KR102018240 B1 KR 102018240B1 KR 1020167017344 A KR1020167017344 A KR 1020167017344A KR 20167017344 A KR20167017344 A KR 20167017344A KR 102018240 B1 KR102018240 B1 KR 102018240B1
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피터 제임스 파치니
스콧 카메론 패로우
기욤 아서 웰치 보두앙
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에피메론 인코포레이티드
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Abstract

화합물 (R)-레티큘린 및 그 합성 전구체의 제조에 이용될 수 있는 방법. 합성 (R)-레티큘린 및 합성 전구체에 유용한 조성물도 제공된다.

Description

(R)-레티큘린 및 그 전구체를 제조하기 위한 조성물 및 방법{COMPOSITIONS AND METHODS FOR MAKING (R)-RETICULINE AND PRECURSORS THEREOF}
본 특허 협력 조약 출원은 35 USC §119(e) 하에서 2013년 12월 4일 출원된 미국 특허 가출원 제61/911,759호 및 2014년 9월 15일 출원된 미국 특허 가출원 제62/050,399호의 이익을 주장하며, 이들 모두는 전체로 본원에 참조로 통합된다.
본원에 개시된 조성물 및 방법은 2차 대사 물질 및 그 제조 공정에 관한 것이다. 더 구체적으로, 본 발명은 (R)-레티큘린과 이의 특정 전구체, 및 (R)-레티큘린과 이러한 전구체를 제조하기 위한 방법 및 조성물에 관한 것이다.
다음 단락들은 본 발명의 배경으로서 제공된다. 그러나, 다음 단락들이 이하 설명하는 무엇이든 종래 기술 또는 당업자의 지식의 일부임을 인정하는 것은 아니다.
생물의 생화학적 경로는 일반적으로 1차 물질대사의 일부이거나 2차 물질대사의 일부인 것으로 분류된다. 생세포의 1차 물질대사의 일부인 경로는 에너지 생성을 위한 이화작용 또는 세포에 대한 블록 생성 구축을 위한 동화작용에 관여된다. 반면, 2차 대사 물질은 어떠한 명백한 동화 또는 이화 기능을 갖지 않고 생세포에 의해 생성된다. 그러나, 많은 2차 대사 물질은, 예를 들어 치료제 또는 천연 억제제를 포함한 여러 측면에서 유용한 것으로 오랫동안 인식되어 왔다.
2차 대사 물질 (R)-레티큘린은 양귀비(Papaver somniferum) 및 양귀비과, 녹나무과, 포포나무과, 대극과 및 뽕나무과 식물과의 다른 구성원에 의해 생성되며, 모르핀과 코데인을 포함하는 약학적 활성 화합물을 제조하기 위한 원료로서 사용될 수 있다.
(R)-레티큘린은 식물체 내에서 (S)-레티큘린으로부터 생성되는 것으로 알려져 있다. 그러나, 어느 유전자 및 폴리펩티드가 변환 반응(들)의 촉매작용에 관여되는지는 불분명하다.
현재 (R)-레티큘린은 양귀비와 같은 천연 원료로부터 채취될 수 있다. 대안적으로, (R)-레티큘린은 합성에 의해 제조될 수 있다. 그러나, 기존의 (R)-레티큘린 제조 방법은 (R)-레티큘린의 낮은 수율 및/또는 고가의 비용 문제를 겪는다. (S)-레티큘린으로부터 (R)-레티큘린을 생합성적으로 만드는 방법은 존재하지 않는다. 따라서, 본 기술분야에서 (R)-레티큘린의 합성을 위한 개선된 방법에 대한 필요성이 존재한다.
다음 단락들은 이어지는 보다 상세한 설명을 읽는 이에게 소개하고자 함이며, 본 발명의 청구 요지를 정의하거나 제한하고자 하는 것은 아니다.
본 발명은 2차 대사 물질 (R)-레티큘린 및 이의 특정 전구체, 뿐만 아니라 (R)-레티큘린 및 이의 특정 전구체를 제조하는 방법에 관한 것이다.
따라서, 본 발명은 적어도 하나의 양태에서, (R)-레티큘린 또는 (R)-레티큘린의 전구체를 제조하는 방법으로서,
(a) 벤질이소퀴놀린 유도체를 제공하는 단계; 및
(b) 벤질이소퀴놀린 유도체를, 벤질이소퀴놀린 유도체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서, 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하는, 방법의 적어도 하나의 구현예를 제공한다.
본 발명은 적어도 하나의 양태에서, (R)-레티큘린 또는 그 전구체를 제조하는 방법으로서,
(a) 벤질이소퀴놀린 유도체를 제공하는 단계; 및
(b) 벤질이소퀴놀린 유도체를, 벤질이소퀴놀린 유도체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서, 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하되,
벤질이소퀴놀린 유도체는 화학식 (I)
[화학식 I]
Figure 112016062482227-pct00001
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타냄)을 가지며,
(R)-레티큘린 전구체는 화학식 (II)
[화학식 II]
Figure 112016062482227-pct00002
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타내며, 단, 화학식 (II)는 (R)-레티큘린을 제외함)를 갖는 방법의 적어도 하나의 구현예를 더 제공한다.
바람직한 구현예에 있어서, 벤질이소퀴놀린 유도체에서 R1은 메톡시기이고, R2는 수산기이고, R3는 수산기이고, R4는 메톡시기이며, R5는 메틸기로서, (S)-레티큘린으로도 알려진 화학식 (III)을 제공한다.
[화학식 III]
Figure 112016062482227-pct00003
더 바람직한 구현예에서, 효소 혼합물은, 벤질이소퀴놀린 유도체를 산화시켜 화학식 (IV)
[화학식 IV]
Figure 112016062482227-pct00004
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타냄)의 산화 벤질이소퀴놀린 유도체를 형성할 수 있는 제1 폴리펩티드, 및
산화 벤질이소퀴놀린 유도체 (IV)를 환원시켜 (R)-레티큘린 또는 화학식 (II)
[화학식 II]
Figure 112016062482227-pct00005
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타내며, 단, 화학식 (II)는 (R)-레티큘린을 제외함)의 (R)-레티큘린 전구체를 형성할 수 있는 제2 폴리펩티드를 포함한다.
더 바람직한 구현예에서, 효소 혼합물은 (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 포함한다.
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체를 산화시켜 산화 벤질이소퀴놀린 유도체를 형성할 수 있는 제1 폴리펩티드는 시토크롬 P450이고, 산화 벤질이소퀴놀린 유도체를 환원시켜 (R)-레티큘린 또는 (R)-레티큘린 전구체를 형성할 수 있는 제2 폴리펩티드는 알도-케토 환원효소(AKR)이다.
본 발명에 따르면, 상기 방법은, 식물, 식물 세포 배양체, 미생물, 및 무세포 시스템에서의 수행을 포함하여(그러나, 이들로 한정되는 것은 아님), 생체 내에서 또는 생체 외에서 수행될 수 있다.
본원에서는, CYP450 및 AKR, 또는 이들의 혼합물로 이루어진 군으로부터 선택되는 효소를 제조하는 방법으로서,
(a) (i) CYP450 및 AKR로 이루어진 군으로부터 선택되는 하나 이상의 폴리펩티드를 암호화하는 하나 이상의 핵산 서열; 및
(ii) 숙주 세포에서 발현을 제어할 수 있는 하나 이상의 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 제공하는 단계;
(b) 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR로 이루어진 군으로부터 선택되는 폴리펩티드를 생성하는 단계; 및
(c) 숙주 세포로부터 CYP450 및 AKR로 이루어진 군으로부터 선택되는 폴리펩티드를 회수하는 단계를 포함하는, 방법이 더 제공된다.
본원에서는, (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 제조하는 방법으로서,
(a) (i) CYP450 폴리펩티드를 암호화하는 제1 핵산 서열;
(ii) AKR 폴리펩티드를 암호화하는 제2 핵산 서열; 및
(iii) 숙주 세포에서 발현을 제어할 수 있는 하나 이상의 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 제공하는 단계;
(b) 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR을 생성하고, (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 생성하는 단계; 및
(c) (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 회수하는 단계를 포함하는, 방법이 더 제공된다.
바람직한 구현예에서, 제1 핵산 서열 및 제2 핵산 서열은 CYP450 및 AKR을 포함하는 융합 폴리펩티드를 생성하기 위해 작동 가능하게 연결된다.
본원에서는, (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 제조하는 방법으로서,
(a) CYP450 폴리펩티드를 암호화하는 제1 핵산 서열 및 세포에서 이 제1 핵산 서열의 발현을 제어하는 제1 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 제1 키메라 핵산 서열을 제공하는 단계;
(b) AKR 폴리펩티드를 암호화하는 제2 핵산 서열 및 세포에서 이 제2 핵산 서열의 발현을 제어하는 제2 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 제2 키메라 핵산 서열을 제공하는 단계;
(c) 제1 키메라 핵산 서열 및 제2 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR을 생성하고, (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 생성하는 단계; 및
(d) (R)-레티큘린 또는 화학식 (II)의 (R)-레티큘린 전구체를 회수하는 단계를 포함하는, 방법이 더 제공된다.
본 발명은, (R)-레티큘린을 제조하기 위한 조성물로서, (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 포함하는 효소 혼합물을 포함하는 조성물을 더 제공한다.
바람직한 구현예에서, 효소 혼합물은 (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 포함한다.
더 바람직한 구현예에서, (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드는 시토크롬 P450이고, 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드는 알도-케토 환원효소(AKR)이다.
본 발명은, (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 암호화하는 핵산 서열을 포함하는 조성물을 더 제공한다. 바람직한 구현예에서, 핵산 서열은, (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 동시에, 시토크롬 P450 및 알도-케토 환원효소, 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 암호화하는 핵산 서열이다.
본 발명은, AKR 및/또는 CYP450을 암호화하는 핵산 서열을 이용하여, 샘플, 예컨대 식물 세포를 포함하는 샘플 내에서 이들의 존재 유무를 검출하는 방법, AKR 및/또는 CYP450을 암호화하는 핵산 서열을 이용하여 식물 세포 및 다른 세포에서 AKR 및/또는 CYP450의 발현을 조절하는 방법, 및 식물 개체에서 유전적으로 연결된 AKR 및/또는 CYP450 유전자의 분리를 평가하기 위한 표지로서 AKR 및/또는 CYP450을 암호화하는 핵산 서열을 이용하는 방법을 더 포함한다.
다른 구현예에서, 본 발명은, AKR 및/또는 CYP450을 암호화하는 핵산 서열의 존재 유무를 검출하는 방법으로서,
(a) AKR 및/또는 CYP450을 암호화하는 핵산 서열을 포함하는 것으로 의심되는 샘플을 제공하는 단계; 및
(b) AKR 및/또는 CYP450을 암호화하는 뉴클레오티드 서열의 존재에 대해 샘플을 분석하는 단계를 포함하는, 방법을 제공한다.
다른 구현예에서, 본 발명은, AKR 및/또는 CYP450을 자연적으로 발현하는 세포에서 핵산 서열의 발현을 조절하는 방법으로서,
(a) AKR 및/또는 CYP450을 자연적으로 발현하는 세포를 제공하는 단계;
(b) 세포에 돌연변이를 일으키는 단계;
(c) 세포를 성장시켜 복수의 세포를 얻는 단계; 및
(d) 복수의 세포가, 조절된 수준의 AKR 및/또는 CYP450을 포함하는 세포를 포함하는지 결정하는 단계를 포함하는, 방법을 제공한다.
또 다른 구현예에서, 본 발명은, 세포에서 AKR 및/또는 CYP450의 발현을 감소시키는 방법으로서,
(a) AKR 및/또는 CYP450을 발현하는 세포를 제공하는 단계; 및
(b) 세포에서 AKR 및/또는 CYP450의 발현을 침묵시키는 단계를 포함하는, 방법을 제공한다.
본 발명의 다른 특징들 및 장점들은 다음의 상세한 설명으로부터 명백해질 것이다. 그러나, 본 발명의 사상 및 범위 내에서의 다양한 변경 및 수정은 상세한 설명으로부터 당업자에게 명백해질 것이므로, 상세한 설명은 본 발명의 바람직한 구현을 나타내면서, 단지 예시로써 제공된다는 것을 이해해야 한다.
이하 도면에 관하여 설명된 단락에서 본 발명이 제공된다. 본원에 제공된 도면은 예시 목적으로 제공되며, 본 발명을 제한하고자 하는 것은 아니다.
도 1은 다양한 벤질이소퀴놀린 전구체의 (R)-레티큘린, (R)-레티큘린 전구체, 모르핀 및 살루타리딘으로의 합성 경로를 도시한다. 표시된 화합물의 화학 구조가 포함되어 있다.
도 2는 (S)-레티큘린 및 이의 합성 중간체들로부터 (R)-레티큘린을 제조하기 위한 합성 경로를 도시하다. 합성 중간체 및 합성 중간체의 화학적 변환의 촉매작용을 할 수 있는 효소의 화학 구조가 포함되어 있다.
도 3은 실시예 1에서 더 설명되는 바와 같이, (S)-레티큘린에서 (R)-레티큘린으로의 변환을 제공하는 본 발명의 구현예의 일련의 HPLC 기록을 도시한다.
도 4는 실시예 2에서 더 설명되는 바와 같이, (S)-레티큘린에서 1,2-디하이드로레티큘린으로의 변환을 제공하는 본 발명의 구현예의 일련의 HPLC 기록을 도시한다.
도 5는 실시예 3에서 더 설명되는 바와 같이, 1,2-디하이드로레티큘린에서 (R)-레티큘린으로의 변환을 나타내는 본 발명의 구현예의 일련의 HPLC 기록을 도시한다.
도 6은 실시예 4에서 더 설명되는 바와 같이, (S)-N-메틸코클라우린에서 (R)-N-메틸코클라우린으로의 변환을 나타내는 본 발명의 구현예의 일련의 HPLC 기록을 도시한다.
도 7은 실시예 5에서 더 설명되는 바와 같이, 유전자 침묵 실험에 관하여 얻어진 결과를 도시한다. REPI 유전자에서 두 개의 다른 영역(도 7a, 도 7c) 및 COR1.3 유전자의 하나의 영역(도 7b)을 표적으로 하였다. 각각의 도 7a 내지 도 7c에서, 상이한 그림들은 다음을 나타낸다: (그림 A) pTRV2 구조체를 조립하는 데 사용되는 REPI 또는 COR1.3 cDNA의 단편(회색 박스). 검은 박스는 암호 영역을 나타내는 반면, 검은 선은 측면 미번역 영역이다. 화살표는 qRT-PCR 분석에 사용된 유전자 프라이머의 결합(annealing) 부위를 나타낸다. (그림 B) pTRV2-REPI-a, pTRV2-REPI-5', 또는 pTRV2-COR1.3 구조체를 가지고 있는 근두암종균(Agrobacterium tumefaciens)이 침투된 개별 식물로부터 추출된 전체 RNA를 이용한 RT-PCR에 의한 pTRV2 벡터의 검출을 보여주는 브롬화 에티듐 염색 아가로오스 겔, 또는 pTRV2 빈 벡터 대조군. PCR 프라이머(TRV2-MCS)는 pTRV2의 다중 복제 부위(MCS)의 측면 영역에 결합하도록 설계되었다. (그림 C) 대조군(pTRV2) 대비 REPI-침묵(pTRV2-REPI-a; pRTV2-REPI-5') 또는 COR1.3-침묵(pRTV2-COR1.3) 식물의 줄기 및 뿌리에서의 상대적 REPI 또는 COR1.3 전사 수준. (그림 D) 대조군(pTRV2) 대비 REPI-침묵 (pTRV2-REPI-a; pRTV2-REPI-5') 또는 COR1.3 침묵(pRTV2-COR1.3) 식물의 주요 알칼로이드 프로파일을 보여주는 총 이온 크로마토그램. (그림 E) 주요 유액 알칼로이드와 대조군(pTRV2) 대비 REPI-침묵(pTRV2-REPI-a; pRTV2-REPI-5') 식물 또는 COR1.3-침묵(pRTV2-COR1.3) 식물에서 억제된 수준을 나타내는 기타 알칼로이드의 상대 존재비. (그림 F) 대조군(pTRV2) 대비 REPI-침묵(pTRV2-REPI-a; pRTV2-REPI-5') 식물 또는 COR1.3-침묵(pRTV2-COR1.3) 식물에서의 (S)-레티큘린 대 (R)-레티큘린의 비. 별표는 쌍을 이루지 않은 양방 스튜던트 t 검정(p<0.05)을 이용하여 결정된 상당한 차이를 나타낸다. 막대는 6개의 개별적으로 침투된 식물 각각에 대한 3개의 기술적 복제로부터 얻은 평균 ± 표준 편차를 나타낸다.
도 8은 실시예 6에서 더 설명되는 바와 같이, 환원제 및 산화제의 존재 시 AKR 폴리펩티드의 활성 평가 시 얻은 결과를 도시한다. 도 8a는 Papaver somniferum 레티큘린 에피머화 효소(REPI)의 1,2-디하이드로레티큘린 환원효소(PsDRR) 성분의 활성을 나타낸다. NADH 또는 NADPH의 존재 시, PsDRR은 1,2-디하이드로레티큘린 [1]을 (R)-레티큘린 [2]로 변환시킨다(도 8a, 그림 A). NAD+ 또는 NADP+의 존재 시, PsDRR은 (R)-레티큘린 [2]를 1,2-디하이드로레티큘린 [1]로 변환시킨다(도 8a, 그림 B). 도 8b는 Papaver rhoeas로부터 1,2-디하이드로레티큘린 환원효소(PrDRR)의 활성을 나타낸다. NADH 또는 NADPH의 존재 시, PrDRR은 1,2-디하이드로레티큘린 [1]을 (R)-레티큘린 [2]로 변환시킨다(도 8b, 그림 A). NAD+ 또는 NADP+의 존재 시, PrDRR은 (R)-레티큘린 [2]를 1,2-디하이드로레티큘린 [1]로 변환시킨다(도 8b, 그림 B).
도 9는 실시예 7에서 더 설명되는 바와 같이, CYP450 및 AKR 폴리펩티드의 pH 의존성 평가 시 얻은 결과를 도시한다. NADPH의 존재 시(PsDRS 정반응) 및 NADP+의 존재 시(PsDRS 역반응) Papaver somniferum CYP450(PsDRS) 및 AKR을 사용하여 얻은 결과들이 도시되어 있다(그림 A). NADPH의 존재 시(PrDRS 정반응) 및 NADP+의 존재 시(PrDRS 역반응) Papaver rhoeas CYP450(PrDRS) 및 AKR을 사용하여 얻은 결과들 또한 도시되어 있다(그림 B).
도 10은 실시예 8에서 더 설명되는 바와 같이, 바이러스 유도 유전자 침묵(VIGS)을 받은 양귀비 식물에서 REPI 및 COR 전사 수준의 공동 억제를 도시한다. COR의 침묵을 표적으로 한 식물(pTRV2-COR1.3)은 COR의 상당한 억제를 나타내었고(도 10 - 아래 그림), 추가적으로 REPI의 억제를 나타내었다(도 10 - 위 그림). REPI와 COR 모두에서 발견되는 보존 영역을 이용하여 REPI의 침묵을 표적으로 한 식물(pTRV2-REPIa) 또한 COR(도 10 - 아래 그림) 및 REPI(도 10 - 위 그림)의 상당한 억제를 나타내었다. REPI의 고유 영역, 또한 COR에서 발견되지 않는 영역을 이용하여 REPI의 침묵을 표적으로 한 식물(pTRV2-REPIb)은 COR의 공동-침묵을 나타내지 않았다(도 10 - 아래 그림). pTRV2는 빈 벡터 대조군이다. 별표는 쌍을 이루지 않은 스튜던트 t 검정(P<0.05)을 이용하여 대조군에 비해 상당히 다른 값을 나타낸다. 에러바는 평균 ± 표준 편차를 나타낸다.
각각의 청구 요지의 구현예를 제공하기 위해 다양한 조성물 및 방법을 이하 설명한다. 이하 설명된 어떠한 구현예도 청구 요지를 제한하는 것은 아니며, 임의의 청구 요지는 이하 설명된 것과 다른 방법, 공정, 조성물 또는 시스템을 포함할 수 있다. 청구 요지가, 이하 설명된 어느 하나의 조성물, 방법, 시스템 또는 공정의 모든 특징을 갖는 조성물 또는 방법으로 한정되거나, 이하 설명된 다수의 또는 모든 조성물, 시스템 또는 방법에 공통된 특징으로 한정되는 것은 아니다. 이하 설명된 조성물, 시스템, 방법 또는 공정은 임의의 청구 요지의 구현예가 아닐 수 있다. 본 문헌에서 청구되지 않은 이하 설명된 조성물, 시스템, 방법 또는 공정에 개시된 임의의 요지는 다른 보호 수단, 예를 들어 계속 특허 출원의 요지일 수 있으며, 본 문헌에서의 개시에 의해 출원인, 발명자 또는 특허권자가 임의의 이러한 요지를 포기하거나 거부하거나 공중에게 바치고자 하는 것은 아니다.
본원에 사용된 "실질적으로", "필수적으로", "약" 및 "대략"과 같은 정도의 용어는 최종 결과가 크게 변하지 않을 정도로 합리적인 양의 수식된 용어의 편차를 의미한다는 점에 주목해야 한다. 이러한 정도의 용어는, 이러한 편차가 수식된 용어의 의미를 무효로 하지 않는다면, 수식된 용어의 편차를 포함하는 것으로 이해해야 한다.
본원에 사용된 표현 "및/또는"은 포함적 논리합을 나타내기 위한 것이다. 즉, "X 및/또는 Y"는, 예를 들어 X 또는 Y, 또는 둘 다를 의미하기 위한 것이다. 또 다른 예로서, "X, Y, 및/또는 Z"는 X 또는 Y 또는 Z, 또는 이들의 임의의 조합을 의미하기 위한 것이다.
모든 공개, 특허 및 특허 출원은 각각의 개별 공개와 동일한 정도로 그 전체가 본원에 참조로 통합된다.
전술한 바와 같이, 본 발명은 2차 대사 물질 (R)-레티큘린 및 그 전구체, 뿐만 아니라 (R)-레티큘린 및 그 전구체를 제조하는 방법에 관한 것이다. 본 발명은 또한 (S)-레티큘린의 변환을 유발하여 (R)-레티큘린을 형성하는 반응의 촉매작용을 할 수 있는 특정 효소에 관한 것이다. 본원에 제공된 방법은 (R)-레티큘린 및 그 전구체를 제조하는 신규의 효율적인 수단을 나타낸다. 본원에 제공된 방법은 화학적 합성을 필요로 하지 않으며, 상업적 규모로 수행될 수 있다. 발명자가 아는 한, 본 발명은 일반적으로 (R)-레티큘린 및 그 전구체를 합성할 수 없는 생세포를 이용하여 (R)-레티큘린 및 그 전구체를 제조하는 방법론을 처음으로 제공한다. 이러한 세포는 (R)-레티큘린 및 그 전구체가 경제적으로 추출될 수 있는 원료로서 사용될 수 있다. 본 발명에 따라 제조된 (R)-레티큘린 및 그 전구체는 모르핀 및 코데인을 포함한 제약 조성물의 제조에 특히 유용하다.
따라서, 본 발명은 적어도 하나의 양태에서, (R)-레티큘린 또는 그 전구체를 제조하는 방법으로서,
(a) 벤질이소퀴놀린 유도체를 제공하는 단계; 및
(b) 벤질이소퀴놀린 유도체를, 벤질이소퀴놀린 유도체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서, 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하는, 방법의 적어도 하나의 구현예를 제공한다.
본 발명은 적어도 하나의 양태에서, (R)-레티큘린 또는 (R)-레티큘린의 전구체를 제조하는 방법으로서,
(a) 벤질이소퀴놀린 유도체를 제공하는 단계; 및
(b) 벤질이소퀴놀린 유도체를, 벤질이소퀴놀린 유도체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서, 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하되,
벤질이소퀴놀린 유도체는 화학식 (I)
[화학식 I]
Figure 112016062482227-pct00006
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타냄)을 가지며,
(R)-레티큘린 전구체는 다음의 화학식
[화학식 II]
Figure 112016062482227-pct00007
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타냄)을 갖는 방법의 적어도 하나의 구현예를 더 제공한다.
정의
본원에 사용된 용어 "벤질이소퀴놀린 유도체"는 화학식 (VII)을 갖는 화합물을 의미한다.
[화학식 VII]
Figure 112016062482227-pct00008
여기서, R1, R2, R3 및 R4 각각은 독립적으로 또는 동시에 수소 원자, 수산기, 알킬기(예를 들어 C1-C10 알킬) 또는 알콕시기(예를 들어 C1-C10 알콕시)를 나타내고, R5는 수소 원자 또는 알킬기(예를 들어 C1-C10 알킬)를 나타낸다.
본원에 사용된 용어 "(R)-레티큘린 전구체"는 화학식 (VIII)을 갖는 화합물을 의미한다.
[화학식 VIII]
Figure 112016062482227-pct00009
여기서, R1, R2, R3 및 R4 각각은 독립적으로 또는 동시에 수소 원자, 수산기, 알킬기(예를 들어 C1-C10 알킬) 또는 알콕시기(예를 들어 C1-C10 알콕시)를 나타내고, R5는 수소 원자 또는 알킬기(예를 들어 C1-C10 알킬)를 나타내며, 단, 화학식 (VIII)은 (R)-레티큘린을 제외한다. 즉, 구체적으로 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 메톡시기, R5가 메틸기인 화합물은 본원에 사용된 용어 (R)-레티큘린 전구체로부터 제외된다.
본원에 사용된 용어 "(S)-레티큘린"은 레티큘린의 (S)-광학이성질체 및 화학식 (III)을 갖는 화합물을 의미한다.
[화학식 III]
Figure 112016062482227-pct00010
용어 "산화 벤질이소퀴놀린 유도체"는 화학식 (IX)을 갖는 화합물을 의미한다.
[화학식 IX]
Figure 112016062482227-pct00011
여기서, R1, R2, R3 및 R4 각각은 독립적으로 또는 동시에 수소 원자, 수산기, 알킬기(예를 들어 C1-C10 알킬) 또는 알콕시기(예를 들어 C1-C10 알콕시)를 나타내고, R5는 수소 원자 또는 알킬기(예를 들어 C1-C10 알킬)를 나타낸다.
본원에 사용된 용어 "(R)-레티큘린"은 레티큘린의 (R)-광학이성질체 및 화학 구조식 (V)를 갖는 화합물을 의미한다.
[구조식 V]
Figure 112016062482227-pct00012
본원에 사용된 용어 "1,2-디하이드로레티큘린"은 화학 구조식 (VI)을 갖는 화합물을 의미한다.
[구조식 VI]
Figure 112016062482227-pct00013
본원에서 상호 교환적으로 사용될 수 있는 용어 "(R)-레티큘린 경로" 또는 "(R)-레티큘린 합성 경로"는 도 1에 도시된 (R)-레티큘린의 합성을 위한 물질대사 경로를 의미한다. (R)-레티큘린 경로 내의 제1 화합물이 그 경로에서 제2 화합물의 ""로서 참조된 경우, 본원에서는 제1 화합물의 합성이 제2 화합물의 합성보다 먼저 일어나는 것을 의미한다. 반대로, 제1 화합물이 (R)-레티큘린 경로에서 제2 화합물로부터 "하위(downstream)"로서 참조된 경우, 본원에서는 제2 화합물의 합성이 제1 화합물의 합성보다 먼저 일어나는 것을 의미한다.
본원에 사용된 용어 "효소 혼합물"은 하나 이상의 효소를 포함하는 혼합물을 의미한다. 둘 이상의 효소를 함유한 혼합물에서 효소들은 혼합물을 형성하기 위한 상호작용 또는 조정 없이 독립적으로 생리 활성일 수 있다는 점에 주목해야 한다. 일 구현예에서, 효소 혼합물에 함유된 효소들은 독립적인 비인접 폴리펩티드 사슬들로서 결합 또는 상호작용할 수 있다. 다른 구현예에서, 효소 혼합물은 두 폴리펩티드 간의 융합 폴리펩티드로 제조될 수 있다.
본원에서 상호 교환적으로 사용될 수 있는 용어 "시토크롬 P450", "CYP450" 또는 "P450"은, (i) 예를 들어, 서열 번호 219 내지 서열 번호 321, 서열 번호 325, 및 서열 번호 338을 포함하는 본원에 기재된 임의의 CYP450 폴리펩티드를 구성하는 아미노산 서열과 실질적으로 동일하거나, (ii) 동의 코돈의 사용이 아니라면, 적어도 적당히 엄격한 조건에서 본원에 기재된 임의의 CYP450 폴리펩티드를 암호화하는 임의의 핵산 서열에 혼성화될 수 있는 핵산 서열에 의해 암호화된 일련의 아미노산 잔기를 포함하는 임의의 모든 효소를 의미한다.
본원에서 상호 교환적으로 사용될 수 있는 용어 "알도-케토 환원효소" 또는 "AKR"은, (i) 예를 들어, 서열 번호 59 내지 서열 번호 115, 서열 번호 327, 서열 번호 329, 서열 번호 330, 및 서열 번호 340을 포함하는 본원에 기재된 임의의 AKR 폴리펩티드를 구성하는 아미노산 서열과 실질적으로 동일하거나, (ii) 동의 코돈이 아니라면, 적어도 적당히 엄격한 조건에서 본원에 기재된 임의의 AKR 폴리펩티드를 암호화하는 임의의 핵산 서열에 혼성화될 수 있는 핵산 서열에 의해 암호화된 일련의 아미노산 잔기를 포함하는 임의의 모든 효소를 의미한다.
본원에 사용된 용어 "핵산 서열"은 자연적으로 존재하는 염기, 당 및 당간의(골격) 연결로 이루어진 일련의 뉴클레오시드 또는 뉴클레오티드 단량체를 의미한다. 이 용어는 또한 비자연적으로 존재하는 단량체 또는 그 일부를 포함하는 변형되거나 치환된 서열을 포함한다. 본 발명의 핵산 서열은 데옥시리보핵산 서열(DNA) 또는 리보핵산 서열(RNA)일 수 있으며, 아데닌, 구아닌, 시토신, 티미딘 및 우라실을 포함하는 자연적으로 존재하는 염기를 포함할 수 있다. 이 서열은 변형 염기를 함유할 수도 있다. 이러한 변형 염기의 예는 아자 및 데아자 아데닌, 구아닌, 시토신, 티미딘 및 우라실, 및 크산틴 및 하이포크산틴을 포함한다.
본원에서 상호 교환적으로 사용된 용어 "CYP450을 암호화하는 핵산 서열" 및 "CYP450 폴리펩티드를 암호화하는 핵산 서열"은, 예를 들어 서열 번호 116 내지 서열 번호 218, 서열 번호 324, 및 서열 번호 337을 포함하여 CYP450 폴리펩티드를 암호화하는 임의의 모든 핵산 서열을 의미한다. CYP450 폴리펩티드를 암호화하는 핵산 서열은, (i) 본원에 기재된 CYP450 폴리펩티드 서열과 실질적으로 동일한 폴리펩티드를 암호화하거나, (ii) 동의 코돈의 사용이 아니라면, 적어도 적당히 엄격한 혼성화 조건에서 본원에 기재된 임의의 CYP450 핵산 서열에 혼성화되거나 적어도 적당히 엄격한 조건에서 이러한 핵산 서열에 혼성화될 임의의 모든 핵산 서열을 더 포함한다.
본원에서 상호 교환적으로 사용된 용어 "AKR을 암호화하는 핵산 서열" 및 "AKR 폴리펩티드를 암호화하는 핵산 서열"은, 예를 들어 서열 번호 1 내지 서열 번호 58, 서열 번호 326, 서열 번호 328, 및 서열 번호 339를 포함하여 AKR 폴리펩티드를 암호화하는 임의의 모든 핵산 서열을 의미한다. AKR 폴리펩티드를 암호화하는 핵산 서열은, (i) 본원에 기재된 AKR 폴리펩티드 서열과 실질적으로 동일한 폴리펩티드를 암호화하거나, (ii) 동의 코돈의 사용이 아니라면, 적어도 적당히 엄격한 혼성화 조건에서 본원에 기재된 임의의 AKR 핵산 서열에 혼성화되거나 적어도 적당히 엄격한 조건에서 이러한 핵산 서열에 혼성화될 임의의 모든 핵산 서열을 더 포함한다.
"실질적으로 동일한"이란 용어는, 두 폴리펩티드 서열이 바람직하게는 적어도 70% 동일, 더 바람직하게는 적어도 85% 동일, 가장 바람직하게는 적어도 95% 동일, 예를 들어 96%, 97%, 98% 또는 99% 동일한 것을 의미한다. 두 폴리펩티드 서열 사이의 동일성의 백분율을 결정하기 위해, 예를 들어 Smith and Waterman (Adv. Appl. Math., 1981, 2: 482)에 의해 수정된 Needleman and Wunsch (J. Mol. Biol., 1970, 48: 443)의 정렬 방법을 이용하여 두 서열 간에 가장 높은 순서 일치율이 얻어지도록 이러한 두 서열의 아미노산 서열이 정렬되고 두 서열 간에 동일한 아미노산의 수가 결정된다. 두 아미노산 서열 간의 백분율 동일성을 계산하는 방법은 일반적으로 알려진 기술이며, 예를 들어 Carillo and Lipton (SIAM J. Applied Math., 1988, 48:1073)이 설명한 방법 및 Computational Molecular Biology (Lesk 편저, Oxford University Press, New York, 1988), Biocomputing: Informatics and Genomics Projects에 설명된 방법들을 포함한다. 일반적으로, 이러한 계산을 위해 컴퓨터 프로그램이 이용될 것이다. 이와 관련하여 사용될 수 있는 컴퓨터 프로그램은 GCG (Devereux et al., Nucleic Acids Res., 1984, 12: 387) BLASTP, BLASTN 및 FASTA (Altschul et al., J. Molec. Biol., 1990:215:403)를 포함하지만, 이들로 한정되는 것은 아니다. 두 폴리펩티드 간의 백분율 동일성을 결정하기 위한 특히 바람직한 방법은, 10의 간극 개방 페널티와 0.1의 간극 확장 페널티를 이용해서 두 서열 중 하나의 총 길이의 적어도 50%가 정렬에 포함된 두 서열 간에 가장 높은 순서 일치율이 얻어지는, BLOSUM 62 scoring matrix (Henikoff S & Henikoff, J G, 1992, Proc. Natl. Acad. Sci. USA 89: 10915-10919)와 더불어 Clustal W algorithm (Thompson, J D, Higgines, D G and Gibson T J, 1994, Nucleic Acid Res 22(22): 4673-4680)을 포함한다.
"적어도 적당히 엄격한 혼성화 조건"은, 용액 내 두 개의 상호 보완적인 핵산 분자 사이에 선택적 혼성화를 촉진하는 조건이 선택되는 것을 의미한다. 혼성화는 핵산 서열 분자 전부 또는 일부에 발생할 수 있다. 일반적으로 혼성화 부분은 길이로 적어도 15(예를 들어, 20, 25, 30, 40 또는 50) 뉴클레오티드이다. 당업자는 핵산 2중가닥 또는 혼성체의 안정성이 나트륨 함유 완충제에서 나트륨 이온 농도 및 온도의 함수인 Tm(Tm=81.5℃-16.6(LoglO [Na+])+0.41(% (G+C)-600/l), 또는 유사식)에 의해 결정된다는 것을 알 것이다. 따라서, 세정 조건에서 혼성체 안정성을 결정하는 파라미터는 나트륨 이온 농도 및 온도이다. 알려진 핵산 분자와 유사하지만 동일하지는 않은 분자를 식별하기 위해 1% 불일치는 약 1℃의 Tm 감소를 초래하는 것으로 가정될 수 있다. 예를 들어, 95%가 넘는 동일성을 갖는 핵산 분자를 탐색할 경우 최종 세정 온도는 약 5℃만큼 감소될 것이다. 이러한 고려 사항들에 근거하여, 당업자는 적절한 혼성화 조건을 용이하게 선택할 수 있을 것이다. 바람직한 구현예에서는, 엄격한 혼성화 조건이 선택된다. 엄격한 혼성화를 달성하기 위해 일례로 다음의 조건이 사용될 수 있다: Tm(상기 식에 기초)-5℃에서 5×염화나트륨/구연산나트륨(SSC)/5×덴하르트 용액/1.0% SDS로 혼성화 이후 60℃에서 0.2×SSC/0.1% SDS의 세정. 적당히 엄격한 혼성화 조건은 42℃에서 3×SSC에서의 세정 단계를 포함한다. 그러나, 대안적인 완충제, 염 및 온도를 이용하여 동등한 엄격성이 달성될 수 있는 것으로 이해된다. 혼성화 조건에 관한 추가 지침은 Current Protocols in Molecular Biology, John Wiley & Sons, N.Y., 1989, 6.3.1-6.3.6 및 Sambrook et al., Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989, Vol. 3에서 확인할 수 있다.
본원에서 핵산 서열의 문맥에 사용된 용어 "키메라(chimeric)"는 자연적으로 연결되지 않은 적어도 두 개의 연결된 핵산 서열을 의미한다. 키메라 핵산 서열은 천연 유래가 다른 연결된 핵산 서열을 포함한다. 예를 들어, COR 단백질을 암호화하는 핵산 서열에 연결된 효모 프로모터를 구성하는 핵산 서열은 키메라로 간주된다. 키메라 핵산 서열은, 자연적으로 연결되지 않는다면, 천연 유래가 동일한 핵산 서열을 포함할 수도 있다. 예를 들어, 특정 세포 타입으로부터 얻은 프로모터를 구성하는 핵산 서열은, 동일한 세포 타입으로부터 얻었지만 일반적으로는 그 프로모터를 구성하는 핵산 서열에 연결되지 않은 폴리펩티드를 암호화하는 핵산 서열에 연결될 수 있다. 키메라 핵산 서열은 비자연적으로 존재하는 임의의 핵산 서열에 연결된 자연적으로 존재하는 임의의 핵산 서열을 포함하는 핵산 서열도 포함한다.
본원에서 상호 교환적으로 사용될 수 있는 용어 "실질적으로 순수한" 및 "격리된"은 자연적으로 함께 하는 성분들로부터 분리된 화합물, 예를 들어 경로 합성 중간체 또는 폴리펩티드를 설명한다. 일반적으로, 샘플 내에서 총 재료의 적어도 60%, 더 바람직하게는 적어도 75%, 더 바람직하게는 적어도 90%, 95%, 96%, 97%, 또는 98%, 가장 바람직하게는 적어도 99%(부피, 건조되거나 건조되지 않은 무게, 또는 몰 퍼센트 또는 몰 분율 기준)가 관심 있는 화합물인 경우 화합물은 실질적으로 순수하다. 순도는 임의의 적절한 방법에 의해, 예를 들어 폴리펩티드의 경우, 크로마토그래피, 겔 전기영동 또는 HPLC 분석법에 의해 측정될 수 있다.
본원에서 효소 또는 단백질과 관련하여 사용된 용어 "회수된"은 효소 또는 단백질의 다소 순수한 형태를 의미한다.
본원에서 (R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 방법을 설명하는 데 사용된 용어 "생체 내에서"는, 예를 들어 미생물 세포 또는 식물 세포를 포함한 생세포 내에서 벤질이소퀴놀린 유도체를 벤질이소퀴놀린 유도체의 변환에 촉매작용을 할 수 있는 효소와 접촉시켜 (R)-레티큘린 또는 (R)-레티큘린 전구체를 형성하는 것을 의미한다.
본원에서 (R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 방법을 설명하는 데 사용된 용어 "생체 외에서"는, 예를 들어 마이크로웰플레이트, 튜브, 플라스크, 비이커, 탱크, 반응기 등을 제한 없이 포함한 생세포 외부 환경에서 벤질이소퀴놀린 유도체를 벤질이소퀴놀린 유도체의 변환에 촉매작용을 할 수 있는 효소와 접촉시켜 (R)-레티큘린 또는 (R)-레티큘린 전구체를 형성하는 것을 의미한다.
일반적 구현예
(R) -레티큘린 및 (R) -레티큘린 전구체의 합성
본 발명은 적어도 하나의 양태에서, (R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 일 구현예로서,
(a) 벤질이소퀴놀린 유도체를 제공하는 단계; 및
(b) 벤질이소퀴놀린 유도체를, 벤질이소퀴놀린 유도체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서, 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하되,
벤질이소퀴놀린 유도체는 화학식 (I)
[화학식 I]
Figure 112016062482227-pct00014
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타냄)을 가지며,
(R)-레티큘린 전구체는 다음의 화학식 (II)
[화학식 II]
Figure 112016062482227-pct00015
(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타내며, 단, 화학식 (II)는 (R)-레티큘린을 제외함)을 갖는, 적어도 하나의 구현예를 제공한다.
바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수소 원자 또는 수산기, R4가 수산기 또는 메톡시기, R5가 수소 원자 또는 메틸기인 유도체이다.
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 수소 원자인 유도체이다. 이 화합물은 (S)-코클라우린으로도 알려져 있다(도 1 참조).
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 메틸기인 유도체이다. 이 화합물은 (S)-N-메틸-코클라우린으로도 알려져 있다(도 1 참조).
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 수산기, R5가 메틸기인 유도체이다. 이 화합물은 (S)-3'-하이드록시-N-메틸코클라우린으로도 알려져 있다(도 1 참조).
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 메톡시기, R5가 메틸기인 유도체이다. 이 화합물은 (S)-레티큘린으로도 알려져 있다(도 1; 화합물 (III) 참조).
더 바람직한 구현예에서, (R)-레티큘린 유도체 (II)는 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 메틸기인 유도체이다.
더 바람직한 구현예에서, (R)-레티큘린 유도체 (II)는 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 메틸기인 유도체이다. 이 화합물은 (R)-N-메틸코클라우린으로도 알려져 있다(도 1 참조).
더 바람직한 구현예에서, (R)-레티큘린 유도체 (II)는 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 수산기, R5가 메틸기인 유도체이다. 이 화합물은 (R)-3'-하이드록시-N-메틸코클라우린으로도 알려져 있다(도 1 참조).
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 메틸기인 유도체이고, (R)-레티큘린 유도체 (II)는 R1이 메톡시기, R2가 수산기, R3가 수소 원자, R4가 수산기, R5가 메틸기인 유도체이다.
더 바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)은 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 수산기, R5가 메틸기인 유도체이고, (R)-레티큘린 유도체 (II)는 R1이 메톡시기, R2가 수산기, R3가 수산기, R4가 수산기, R5가 메틸기인 유도체이다.
본 발명의 바람직한 구현예에서, (R)-레티큘린을 제조하는 방법으로서,
(a) (S)-레티큘린을 제공하는 단계; 및
(b) (S)-레티큘린을, (S)-레티큘린이 (R)-레티큘린으로 변환될 수 있는 조건에서, (S)-레티큘린을 (R)-레티큘린으로 변환시킬 수 있는 효소 혼합물과 접촉시키는 단계를 포함하는, 방법이 제공된다.
바람직한 구현예에서, 효소 혼합물은 (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 포함한다(도 2 참조).
바람직한 구현예에서, 효소 혼합물은, 벤질이소퀴놀린 유도체 (I)을 산화시켜 화학식 (IV)
[화학식 IV]
Figure 112016062482227-pct00016
(바람직한 구현예에서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, 바람직한 구현예에서, R5는 수소 원자 또는 메틸기를 나타냄)의 산화 벤질이소퀴놀린 유도체를 형성할 수 있는 제1 폴리펩티드, 및 화학식 (IV)의 산화 벤질이소퀴놀린 유도체를 환원시켜 (R)-레티큘린 또는 화학식 (II)(여기서, R1, R2, R3 및 R4 각각은 수소 원자, 수산기 또는 메톡시기를 나타내고, R5는 수소 원자 또는 메틸기를 나타내며, 단, 화학식 (II)는 (R)-레티큘린을 제외함)의 (R)-레티큘린 유도체를 형성할 수 있는 제2 폴리펩티드를 포함한다.
바람직한 구현예에서, 벤질이소퀴놀린 유도체 (I)을 산화시켜 산화 벤질이소퀴놀린 유도체 (IV)를 형성할 수 있는 제1 폴리펩티드는 시토크롬 P450이고, 산화 벤질이소퀴놀린 유도체를 환원시켜 (R)-레티큘린 또는 (R)-레티큘린 유도체를 형성할 수 있는 제2 폴리펩티드는 알도-케토 환원효소(AKR)이다. 특히 바람직한 구현예에서, AKR 폴리펩티드는 P. somniferum, P. bracteatum 및 P. rhoeas로부터 얻거나 얻을 수 있다.
특정 구현예에서, 제1 폴리펩티드 및 제2 폴리펩티드는 두 개의 분리된 폴리펩티드, 즉 공유 화학 결합에 의해 연결되지 않은 폴리펩티드의 형태로 제공된다. 바람직한 특정 구현예에서, 제1 폴리펩티드 및 제2 폴리펩티드는 CYP450 폴리펩티드를 암호화하는 제1 부분 및 AKR 폴리펩티드를 암호화하는 제2 부분을 포함하는 융합 폴리펩티드로서 제조된다. 이러한 융합 폴리펩티드는 재조합형으로 제조될 수 있거나, 서열 번호 323에 명시된 Papaver somniferum 폴리펩티드와 같이 자연적으로 존재하는 융합 폴리펩티드일 수 있다.
본 발명에 따라 사용될 수 있는 CYP450 폴리펩티드의 예는 서열 번호 219 내지 서열 번호 321, 서열 번호 325, 및 서열 번호 338에 명시된 폴리펩티드를 포함한다. 본 발명에 따라 사용될 수 있는 AKR 폴리펩티드의 예는 서열 번호 59 내지 서열 번호 115, 서열 번호 327, 서열 번호 329, 서열 번호 330, 및 서열 번호 340에 명시된 폴리펩티드를 포함한다.
상기 반응은 벤질이소퀴놀린 전구체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서 수행된다. 이하 더 상세히 설명되는 바와 같이, 조건은 생체 내 조건 또는 생체 외 조건을 포함한다. 조건은 더 일반적으로 물과 완충제의 존재를 포함한다. 더 일반적으로, 산화 벤질이소퀴놀린 전구체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시키는 환원 반응을 가능케 하기 위해 환원제가 포함된다. 환원제는 니코틴아미드 아데닌 디뉴클레오티드(NADH)일 수 있으며, 다른 구현예에서, 환원제는 니코틴아미드 아데닌 디뉴클레오티드 인산(NADPH)이다. 더 일반적으로, 벤질이소퀴놀린 유도체를 산화 벤질이소퀴놀린으로 변환시키는 효소를 환원시킬 수 있는 환원효소 및 환원제가 반응에 포함된다. 바람직한 구현예에서, 환원효소는, 예를 들어 양귀비 시토크롬 P450 환원효소와 같이 CYP450을 환원시킬 수 있는 시토크롬 P450 환원효소이며, 환원제는 NADH, 또는 더 바람직하게 NADPH이다. 더욱 주목할 것은, 다양한 pH, 예를 들어 약 pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, 또는 pH 10에서 반응이 수행될 수 있다는 것이다. 본원의 실시예 7에 예시한 바와 같이, 서로 다른 pH의 범위에서 반응을 수행하고 반응 속도를 평가하여 반응에 대한 최적 pH를 확인할 수 있음은 당업자에게 명백할 것이다. 최적의 pH는, 예를 들어 본원에 따라 선택되는 기질 및 효소에 따라 다를 수 있다. 따라서, 단지 예로써, 실시예 7은 (S)-레티큘린에서 1,2-디하이드로레티큘린으로의 변환에 대해 약 pH 8의 최적 pH, 1,2-디하이드로레티큘린에서 (R)-레티큘린으로의 변환에 대해 약 pH 7의 최적 pH, 및 (R)-레티큘린에서 1,2-디하이드로레티큘린으로의 변환에 대해 약 pH 9의 최적 pH를 제시한다.
본원에 따르면, 선택된 반응 조건에 따라, 1,2-디하이드로레티큘린에서 (R)-레티큘린으로의 변환을 포함하는 반응이 역으로 진행되거나, 또는 부분적으로 역으로 진행될 수 있다는 것에 유의해야 한다. 따라서, 실시예 6에 제시된 바와 같이, (R)-레티큘린은 1,2-디하이드로레티큘린으로 변환될 수 있다. 따라서, 본 발명은 1,2-디하이드로레티큘린을 제조하는 방법으로서,
(a) (R)-레티큘린을 제공하는 단계; 및
(b) (R)-레티큘린을, (R)-레티큘린이 1,2-디하이드로레티큘린으로 변환될 수 있는 조건에서, (R)-레티큘린을 1,2-디하이드로레티큘린으로 변환시킬 수 있는 AKR 폴리펩티드와 접촉시키는 단계를 포함하는, 방법을 더 제공한다. 상기 반응을 수행하는 데 사용될 수 있는 AKR 폴리펩티드는 서열 번호 59 내지 서열 번호 115, 서열 번호 327, 서열 번호 329, 서열 번호 330, 및 서열 번호 340에 명시된 임의의 폴리펩티드를 포함한다. 변환을 가능케 하는 반응 조건은 반응 혼합물 내에 산화제, 바람직하게는 NAD+ 또는 NADP+의 존재를 포함한다. 앞서 언급한 바와 같이, 반응에 대한 pH는 최적화될 수 있다. 상기 반응의 가역성은 도 2에 더 도시되어 있다.
바람직한 구현예에서, 벤질이소퀴놀린 유도체를 산화시켜 산화 벤질이소퀴놀린 유도체를 형성할 수 있는 제1 폴리펩티드는 시토크롬 P450이고, 산화 벤질이소퀴놀린 유도체를 환원시켜 (R)-레티큘린 또는 (R)-레티큘린 유도체를 형성할 수 있는 제2 폴리펩티드는 알도-케토 환원효소(AKR)이다. 특히 바람직한 구현예에서, AKR은 P. somniferum, P. bracteatum 및 P. rhoeas로부터 얻거나 얻을 수 있다.
특정 구현예에서, 제1 폴리펩티드 및 제2 폴리펩티드는 두 개의 분리된 폴리펩티드, 즉 공유 화학 결합에 의해 연결되지 않은 폴리펩티드의 형태로 제공된다. 바람직한 특정 구현예에서, 제1 폴리펩티드 및 제2 폴리펩티드는 CYP450 폴리펩티드를 암호화하는 제1 부분 및 AKR 폴리펩티드를 암호화하는 제2 부분을 포함하는 융합 폴리펩티드로서 제조된다. 이러한 융합 폴리펩티드는 재조합형으로 제조될 수 있거나, 서열 번호 323에 명시된 Papaver somniferum 폴리펩티드와 같이 자연적으로 존재하는 융합 폴리펩티드일 수 있다.
본 발명에 따라 사용될 수 있는 CYP450 폴리펩티드의 예는, Papaver somniferum, Papaver rhoeas 및 Papaver bracteatum을 포함하는 Papaver 종; Argemone mexicana를 포함하는 Argemone 종; Berberis thunbergii를 포함하는 Berberis 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Chelidonium majus를 포함하는 Chelidonium 종; Cissampelos mucronata를 포함하는 Cissampelos 종; Cocculus trilobus를 포함하는 Cocculus 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Glaucium flavum을 포함하는 Glaucium 종; Hydrastis canadensis를 포함하는 Hydrastis 종; Jeffersonia diphylla를 포함하는 Jeffersonia 종; Mahonia aquifolium을 포함하는 Mahonia 종; Menispermum canadense를 포함하는 Menispermum 종; Nandina domestica를 포함하는 Nandina 종; Nigella sativa를 포함하는 Nigella 종; Sanguinaria canadensis를 포함하는 Sanguinaria 종; Stylophorum diphyllum을 포함하는 Styplophorum 종; Thalictrum flavum을 포함하는 Thalictrum 종; Tinospora cordifolia를 포함하는 Tinospora 종; 및 Xanthoriza simplicissima를 포함하는 Xanthoriza 종의 다양한 종으로부터 얻을 수 있는 CYP450 폴리펩티드를 포함한다. 상기 폴리펩티드는 구체적으로 서열 번호 219 내지 서열 번호 321, 서열 번호 325, 및 서열 번호 338에 명시된 앞서 언급한 종들로부터 얻은 폴리펩티드를 포함한다. 본 발명에 따라 사용될 수 있는 AKR 폴리펩티드의 예는, Papaver somniferum, Papaver rhoeas 및 Papaver bracteatum을 포함하는 Papaver 종; Argemone mexicana를 포함하는 Argemone 종; Berberis thunbergii를 포함하는 Berberis 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Chelidonium majus를 포함하는 Chelidonium 종; Cissampelos mucronata를 포함하는 Cissampelos 종; Cocculus trilobus를 포함하는 Cocculus 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Glaucium flavum을 포함하는 Glaucium 종; Hydrastis canadensis를 포함하는 Hydrastis 종; Jeffersonia diphylla를 포함하는 Jeffersonia 종; Mahonia aquifolium을 포함하는 Mahonia 종; Menispermum canadense를 포함하는 Menispermum 종; Nandina domestica를 포함하는 Nandina 종; Nigella sativa를 포함하는 Nigella 종; Sanguinaria canadensis를 포함하는 Sanguinaria 종; Stylophorum diphyllum을 포함하는 Styplophorum 종; Thalictrum flavum을 포함하는 Thalictrum 종; Tinospora cordifolia를 포함하는 Tinospora 종; 및 Xanthoriza simplicissima를 포함하는 Xanthoriza 종의 다양한 종으로부터 얻을 수 있는 AKR 폴리펩티드를 포함한다. 상기 폴리펩티드는 구체적으로 서열 번호 59 내지 서열 번호 115, 서열 번호 327, 서열 번호 329, 서열 번호 330, 및 서열 번호 340에 명시된 앞서 언급한 종들로부터 얻은 폴리펩티드를 포함한다.
상기 반응은 벤질이소퀴놀린 전구체가 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환될 수 있는 조건에서 수행된다. 이하 더 상세히 설명되는 바와 같이, 조건은 생체 내 조건 또는 생체 외 조건을 포함한다. 조건은 더 일반적으로 물과 완충제의 존재를 포함한다. 더 일반적으로, 산화 벤질이소퀴놀린 전구체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시키는 환원 반응을 가능케 하기 위해 환원제가 포함된다. 환원제는 니코틴아미드 아데닌 디뉴클레오티드(NADH)일 수 있으며, 다른 구현예에서, 환원제는 니코틴아미드 아데닌 디뉴클레오티드 인산(NADPH)이다. 더 일반적으로, 벤질이소퀴놀린 유도체를 산화 벤질이소퀴놀린으로 변환시키는 효소를 환원시킬 수 있는 환원효소 및 환원제가 반응에 포함된다. 바람직한 구현예에서, 환원효소는 CYP450을 환원시킬 수 있는 시토크롬 P450 환원효소이며, 환원제는 NADH, 또는 더 바람직하게 NADPH이다.
(R) -레티큘린 또는 (R) -레티큘린 유도체의 생체 외 합성
본 발명의 특정 양태에 따르면, 벤질이소퀴놀린 유도체는 생체 외 반응 조건에서 벤질이소퀴놀린 유도체의 효소 촉매 화학 변환을 가능케 하는 반응 조건에서 효소 CYP450 및 AKR의 촉매량과 접촉된다. 이러한 생체 외 반응 조건에서, 초기 반응 구성 성분들은 다소 순수한 형태로 제공되고, 필요한 화학 반응이 실질적으로 진행될 수 있는 조건에서 혼합된다. 실질적으로 순수한 형태의 초기 벤질이소퀴놀린 유도체는 구입할 수 있다. (S)-레티큘린은, 예를 들어 실질적으로 순수한 화합물로서 (예를 들어, Santa Cruz Biotechnology사로부터) 구입하거나, 전구체 화합물로부터 화학적으로 합성되거나, Papaver somniferum 및 원하는 화합물을 포함하는 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과의 다른 구성원을 포함하는 천연 원료로부터 격리될 수 있다. 적합한 양귀비과 구성원은 Papaver, Corydalis, Chelidonium, 및 Romeria 속(genus)에 속하는 종을 포함하지만, 이들로 한정되는 것은 아니다. 이러한 종은 (S)-레티큘린을 만들 수 있으며, Chelidonium majus; Corydalis bulbosa; Corydalis cava; Corydalis ochotenis; Corydalis ophiocarpa; Corydalis platycarpa; Corydalis tuberosa; Papaver armeniacum; Papaver Bracteatum; Papaver cylindricum; Papaver decaisnei; Papaver fugax; Papaver oreophyllum; Papaver orientale; Papaver paeonifolium; Papaver persicum; Papaver pseudo-orientale; Papaver rhoeas; Papaver rhopalothece; Papaver setigerum; Papaver somniferum; Papaver tauricolum; Papaver triniaefolium; 및 Romeria carica 종들로부터 선택되는 식물종을 포함하지만, 이들로 한정되는 것은 아니다. (S)-레티큘린의 화학적 합성은, 예를 들어 S. Teitel and A. Bross, Journal of Heterocyclic Chemistry 5, 825-829, 1968에 설명된 표준 방법을 이용하여 수행될 수 있다.
본원에 따르면, 다소 순수한 형태의 효소는 Papaver somniferum, Papaver bracteatum 및 Papaver rhoeas를 제한 없이 포함하는 천연 원료로부터 격리되거나, 재조합형으로 또는 합성적으로 제조될 수 있다. 따라서, 본원에서, CYP450 및 AKR, 또는 이들의 혼합물로 이루어진 군으로부터 선택되는 효소를 제조하는 방법으로서,
(a) (i) CYP450 및 AKR로 이루어진 군으로부터 선택되는 하나 이상의 폴리펩티드를 암호화하는 하나 또는 둘의 핵산 서열; 및
(ii) 숙주 세포에서 발현을 제어할 수 있는 하나 이상의 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 제공하는 단계;
(b) 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR로 이루어진 군으로부터 선택되는 폴리펩티드를 생성하는 단계; 및
(c) 숙주 세포로부터 CYP450 및 AKR로 이루어진 군으로부터 선택되는 폴리펩티드를 회수하는 단계를 포함하는, 방법이 더 제공된다.
핵산 서열은 이러한 서열을 포함하는 임의의 천연 원료, 예를 들어 식물 원료로부터 얻을 수 있다. 바람직한 식물 원료는 Papaver somniferum, Papaver rhoeas 및 Papaver bracteatum을 포함하는 Papaver 종; Argemone mexicana를 포함하는 Argemone 종; Berberis thunbergii를 포함하는 Berberis 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Chelidonium majus를 포함하는 Chelidonium 종; Cissampelos mucronata를 포함하는 Cissampelos 종; Cocculus trilobus를 포함하는 Cocculus 종; Corydalis chelantifolia를 포함하는 Corydalis 종; Glaucium flavum을 포함하는 Glaucium 종; Hydrastis canadensis를 포함하는 Hydrastis 종; Jeffersonia diphylla를 포함하는 Jeffersonia 종; Mahonia aquifolium을 포함하는 Mahonia 종; Menispermum canadense를 포함하는 Menispermum 종; Nandina domestica를 포함하는 Nandina 종; Nigella sativa를 포함하는 Nigella 종; Sanguinaria canadensis를 포함하는 Sanguinaria 종; Stylophorum diphyllum을 포함하는 Styplophorum 종; Thalictrum flavum을 포함하는 Thalictrum 종; Tinospora cordifolia를 포함하는 Tinospora 종; 및 Xanthoriza simplicissima를 포함하는 Xanthoriza 종을 포함한다. CYP450에 대하여, 상기 식물종으로부터 얻을 수 있거나 얻은 핵산 서열은 서열 번호 116 내지 서열 번호 218, 서열 번호 324, 및 서열 번호 337에 명시된 핵산 서열을 포함한다. AKR에 대하여, 상기 식물종으로부터 얻을 수 있거나 얻은 핵산 서열은 서열 번호 1 내지 서열 번호 58, 서열 번호 326, 서열 번호 328, 및 서열 번호 339에 명시된 핵산 서열을 포함한다. 더 바람직한 구현예에서, 서열 번호 322에 명시된 핵산 서열을 포함하여, CYP450과 AKR 간의 천연 융합 폴리펩티드를 암호화하는 핵산 서열이 사용될 수 있다.
숙주 세포의 성장은 CYP450 및/또는 AKR 폴리펩티드의 생성으로 이어진다. 이후, 예를 들어 이온 교환 크로마토그래피, 크기 배제 크로마토그래피, 친화 크로마토그래피, 소수성 상호작용 크로마토그래피, 역상 크로마토그래피, 겔 여과 등을 포함한 상이한 다양한 단백질 정제 기술에 의해, 다른 숙주 세포 구성요소로부터 폴리펩티드를 회수, 격리, 분리할 수 있다. 단백질 정제에 관한 더 일반적인 지침은, 예를 들어 Cutler, P. Protein Purification Protocols, Humana Press, 2004, 2판에서 확인할 수 있다. 따라서, CYP450 및/또는 AKR 폴리펩티드의 실질적으로 순수한 제조를 얻을 수 있다.
본원에 따르면, 벤질이소퀴놀린 유도체는 벤질이소퀴놀린 유도체의 효소 촉매 화학 변환을 가능케 하는 반응 조건에서 CYP450과 AKR의 하나 이상의 효소의 촉매량과 접촉된다. 바람직한 구현예에서, 이들 제제는 서로 접촉되고 혼합되어 혼합물을 형성한다. 바람직한 구현예에서, 혼합물은 물뿐만 아니라, 완충제, 염, pH 조절제를 포함하여 효소 촉매작용을 촉진하기 위한 추가 제제를 선택적으로 더 포함하는 수성 혼합물이다. 전술한 바와 같이, 반응 혼합물은 NADPH 및 환원효소를 포함하는 것이 특히 바람직하다. 반응은 서로 다른 온도의 범위에서 수행될 수 있다. 바람직한 구현예에서, 반응은 약 18℃와 약 37℃ 사이의 온도에서 수행된다. 생체 외 반응의 종료 시, 다소 순수한 형태로 (R)-레티큘린 또는 (R)-레티큘린 전구체를 얻을 수 있다.
(R) -레티큘린 또는 (R) -레티큘린 전구체의 생체 내 합성
본 발명의 특정 양태에 따르면, 벤질이소퀴놀린 유도체는 생체 내 반응 조건에서 벤질이소퀴놀린 유도체의 효소 촉매 화학 변환을 가능케 하는 반응 조건에서 CYP450과 AKR의 하나 이상의 효소의 촉매량과 접촉된다. 이러한 생체 내 반응 조건에서, 생세포는 (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성하는 방식으로 변형된다. 특정 구현예에서, 생세포는 박테리아 세포 및 진균 세포를 포함한 미생물이다. 다른 구현예에서, 생세포는 식물 및 식물 세포 배양체를 포함한 다세포 생물이다.
일 구현예에서, 생세포는 벤질이소퀴놀린 유도체, (S)-레티큘린, (R)-레티큘린 전구체 또는 (R)-레티큘린을 자연적으로 생성할 수 없는 숙주 세포로 선택된다. 다른 구현예에서, 숙주 세포는 (S)-레티큘린 또는 벤질이소퀴놀린 유도체를 자연적으로 생성할 수 있지만 (R)-레티큘린 또는 (R)-레티큘린 전구체를 자연적으로 생성할 수 없다. 즉, 세포는 (S)-광학이성질체로부터 (R)-광학이성질체로의 에피머화 반응을 자연적으로 수행할 수 없다. 다른 구현예에서, 세포는 벤질이소퀴놀린 유도체 또는 (S)-레티큘린 및 (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성할 수 있지만, (R)-레티큘린 또는 (R)-레티큘린 전구체의 수준은 원하는 것보다 낮아서, (R)-레티큘린 또는 (R)-레티큘린 전구체의 수준은 변형되지 않은 세포에서의 수준에 대해 조절된다. 이러한 세포는 박테리아, 효모, 기타 진균 세포, 식물 세포, 또는 동물 세포를 제한 없이 포함한다.
(R)-레티큘린 또는 (R)-레티큘린 전구체를 생성하기 위해, 본원에서는 (R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 방법으로서,
(a) (i) CYP450 폴리펩티드를 암호화하는 제1 핵산 서열;
(ii) AKR 폴리펩티드를 암호화하는 제2 핵산 서열; 및
(iii) 숙주 세포에서 발현을 제어할 수 있는 하나 이상의 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 제공하는 단계;
(b) 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR을 생성하고, (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성하는 단계; 및
(c) (R)-레티큘린 또는 (R)-레티큘린 전구체를 회수하는 단계를 포함하는, 방법이 더 제공된다.
바람직한 구현예에서, 제1 핵산 서열 및 제2 핵산 서열은, CYP450 및 AKR을 포함하는 융합 폴리펩티드를 생성하기 위해 작동 가능하게 연결된다.
(R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 방법으로서,
(a) CYP450 폴리펩티드를 암호화하는 제1 핵산 서열 및 세포에서 제1 핵산 서열의 발현을 제어하는 제1 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 제1 키메라 핵산 서열을 제공하는 단계;
(b) AKR 폴리펩티드를 암호화하는 제2 핵산 서열 및 세포에서 제2 핵산 서열의 발현을 제어하는 제2 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 제2 키메라 핵산 서열을 제공하는 단계;
(c) 제1 키메라 핵산 서열 및 제2 키메라 핵산 서열을 숙주 세포 내에 도입시키고 숙주 세포를 성장시켜 CYP450 및 AKR을 생성하고, (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성하는 단계; 및
(d) (R)-레티큘린 또는 (R)-레티큘린 전구체를 회수하는 단계를 포함하는, 방법이 더 제공된다.
바람직한 구현예에서, CYP450 및 AKR을 암호화하는 핵산 서열은, Papaver somniferum 및 원하는 화합물을 포함하는 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과의 다른 구성원으로부터 얻을 수 있거나 얻은 CYP450 및 AKR을 암호화하는 핵산 서열로부터 선택된다. 적합한 양귀비과 구성원은 Papaver, Corydalis, Chelidonium, 및 Romeria 속(genus)에 속하는 종을 포함하지만, 이들로 한정되는 것은 아니다. 이러한 종은 (R)-레티큘린을 만들 수 있으며, Chelidonium majus; Corydalis bulbosa; Corydalis cava; Corydalis ochotenis; Corydalis ophiocarpa; Corydalis platycarpa; Corydalis tuberosa; Papaver armeniacum; Papaver Bracteatum; Papaver cylindricum; Papaver decaisnei; Papaver fugax; Papaver oreophyllum; Papaver orientale; Papaver paeonifolium; Papaver persicum; Papaver pseudo-orientale; Papaver rhoeas; Papaver rhopalothece; Papaver setigerum; Papaver somniferum; Papaver tauricolum; Papaver triniaefolium; 및 Romeria carica 종들로부터 선택되는 식물종을 포함하지만, 이들로 한정되는 것은 아니다. 특히 바람직한 구현예에서, CYP450 및 AKR을 암호화하는 핵산 서열은 Papaver somniferum, Papaver bracteatum 및 Papaver rhoeas로부터 얻을 수 있거나 얻은 CYP450 및 AKR을 암호화하는 핵산 서열로부터 선택되는 핵산 서열이다. 더 바람직한 구현예에서, CYP450을 암호화하는 핵산 서열은 본원에서 서열 번호 116 내지 218, 서열 번호 324, 및 서열 번호 337로서 명시된 CYP450을 암호화하는 핵산 서열 중 하나이다. 바람직한 구현예에서, AKR을 암호화하는 핵산 서열은 본원에서 서열 번호 1 내지 서열 번호 58, 서열 번호 326, 서열 번호 328, 및 서열 번호 339로서 명시된 AKR을 암호화하는 핵산 서열 중 하나이다. 특히 바람직한 다른 구현예에서, CYP450 및 AKR을 암호화하는 핵산 서열은 CYP450-AKR 융합 폴리펩티드를 생성할 수 있는 핵산 서열로서, 서열 번호 322에 명시된 서열을 제한 없이 포함한다.
본원에 따르면, CYP450 및 AKR을 암호화하는 핵산 서열은 숙주 세포에서 CYP450 및 AKR의 발현을 제어할 수 있는 핵산 서열에 연결된다. 따라서, 본 발명은 숙주 세포에서 발현을 제어할 수 있는 프로모터에 연결된 CYP450 및 AKR을 암호화하는 핵산 서열을 또한 제공한다. 본원에서 사용될 수 있는 숙주 세포에서 발현을 제어할 수 있는 핵산 서열은 숙주 세포에서 폴리펩티드의 발현을 제어할 수 있는 임의의 전사 프로모터를 포함한다. 일반적으로, 본원에 따라 박테리아 숙주가 선택되는 경우 박테리아 세포에서 얻은 프로모터가 사용되는 반면, 진균 숙주가 선택되는 경우 진균 프로모터가 사용될 것이며, 식물 세포가 선택되는 경우 식물 프로모터가 사용될 것이다. 숙주 세포에서 발현을 제어할 수 있는 다른 핵산 요소는 전사 종결자, 증폭자 등을 포함하며, 이들 모두는 본 발명의 키메라 핵산 서열에 포함될 수 있다. 핵산 서열의 작동 가능한 연결이 프로모터 및 발현을 제어할 수 있는 서열을 5'에서 3' 전사 방향으로 암호 서열에 연결하는 것을 포함한다는 것은 당업자가 이해할 것이다.
본 발명에 따르면, CYP450 및 AKR을 암호화하는 핵산 서열에 연결된 숙주 세포에서 발현을 제어할 수 있는 프로모터를 포함하는 키메라 핵산 서열은 숙주 세포에서의 우수한 발현을 보장하는 재조합 발현 벡터로 통합될 수 있다. 따라서, 본 발명은,
(i) 숙주 세포에서 발현을 제어할 수 있는 핵산 서열; 및
(ii) CYP450을 암호화하는 핵산 서열을 작동 가능하게 연결된 구성요소로서 포함하되, 숙주 세포에서의 발현에 적합한 재조합 발현 벡터를 포함한다.
본 발명은,
(i) 숙주 세포에서 발현을 제어할 수 있는 핵산 서열; 및
(ii) AKR을 암호화하는 핵산 서열을 작동 가능하게 연결된 구성요소로서 포함하되, 숙주 세포에서의 발현에 적합한 재조합 발현 벡터를 포함한다.
본 발명은,
(i) 숙주 세포에서 발현을 제어할 수 있는 핵산 서열; 및
(ii) CYP450 및 AKR을 암호화하는 핵산 서열을 작동 가능하게 연결된 구성요소로서 포함하되, 숙주 세포에서의 발현에 적합한 재조합 발현 벡터를 더 포함한다. "숙주 세포에서의 발현에 적합한"이란 용어는 재조합 발현 벡터가 숙주 세포에서의 발현을 달성하는 데 필요한 유전 인자에 연결된 본 발명의 키메라 핵산 서열을 포함한다는 것을 의미한다. 이와 관련하여 발현 벡터에 포함될 수 있는 유전 인자는 전사 종결 영역, 표지 유전자를 암호화하는 하나 이상의 핵산 서열, 하나 이상의 복제 원점 등을 포함한다. 바람직한 구현예에서, 발현 벡터는 숙주 세포의 게놈에서 벡터 또는 그 일부를 통합하는 데 필요한 유전 인자(예를 들어, 식물 숙주 세포가 사용되는 경우 식물의 핵 게놈으로의 통합을 용이하게 하는 T-DNA 좌우 경계 서열)를 더 포함한다.
본 발명에 따라, 발현 벡터는 표지 유전자를 더 포함할 수 있다. 본 발명에 따라 사용될 수 있는 표지 유전자는, 모든 선택 및 선별 표지 유전자를 포함하여, 형질 전환된 세포를 형질 전환되지 않은 세포와 구별할 수 있도록 하는 모든 유전자를 포함한다. 표지 유전자는, 예를 들어 카나마이신 또는 암피실린에 대한 항생 저항 표지와 같은 저항 표지일 수 있다. 육안 검사를 통해 형질 전환체를 식별하는 데 이용될 수 있는 선별 표지는 β-글루쿠로니다아제(GUS)(미국 특허 제5,268,463호 및 제5,599,670호) 및 녹색 형광 단백질(GFP)(Niedz et al., 1995, Plant Cell Rep., 14: 403)을 포함한다.
특히 편리하게 사용될 수 있는 하나의 숙주 세포는 대장균이다. 대장균 벡터의 제조는 일반적으로 알려진 기술, 예컨대 제한효소 분해, 리게이션, 겔 전기영동, DNA 염기서열 분석, 중합효소 연쇄 반응(PCR) 및 기타 방법을 이용하여 달성될 수 있다. 재조합 발현 벡터를 제조하는 데 필요한 필수 단계를 수행하기 위해 다양한 클로닝 벡터가 이용될 수 있다. 대장균에서 작용하는 복제 시스템을 가진 벡터들 중에는, pBR322, pUC 계열 벡터, M13 mp 계열 벡터, pBluescrpt 등과 같은 벡터들이 있다. 일반적으로, 이러한 클로닝 벡터는 형질 전환된 세포의 선택을 가능케 하는 표지를 포함한다. 핵산 서열들이 이들 벡터 내에 도입될 수 있고, 그 벡터들은 수용성 세포(competent cells)를 제조하거나 전기천공법 또는 당업자에게 잘 알려진 기타 방법을 이용하여 대장균 내에 도입될 수 있다. 대장균은 Luria-Broth 배지를 제한 없이 포함한 적절한 배지에서 성장하고 채취될 수 있다. 재조합 발현 벡터는 세포의 채취 및 용해 시 세포로부터 용이하게 회수될 수 있다. 재조합 벡터의 제조 및 재조합 생물의 성장에 관한 더 일반적인 지침은, 예를 들어 Sambrook et al., Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, 2001, 3판에서 확인할 수 있다.
본 발명은 숙주 세포를 더 포함하며, 이 숙주 세포는 CYP450 및 AKR로 이루어진 군으로부터 선택되는 하나 이상의 폴리펩티드를 암호화하는 하나 이상의 핵산 서열을 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 포함한다. 전술한 바와 같이, 숙주 세포는 바람직하게 벤질이소퀴놀린 유도체, (S)-레티큘린, 또는 (R)-레티큘린 또는 (R)-레티큘린 전구체를 자연적으로 생성할 수 없는 숙주 세포이다. 다른 구현예에서, 숙주 세포는 (S)-레티큘린 또는 벤질이소퀴놀린 유도체를 자연적으로 생성할 수 있지만 (R)-레티큘린 또는 (R)-레티큘린 전구체를 자연적으로 생성할 수 없다. 다른 구현예에서, 숙주 세포는 벤질이소퀴놀린 유도체, (S)-레티큘린, 또는 (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성할 수 있지만, (R)-레티큘린 또는 (R)-레티큘린 전구체의 수준은 원하는 것보다 낮아서, (R)-레티큘린 또는 (R)-레티큘린 전구체의 수준은 본래의 변형되지 않은 세포에서의 (R)-레티큘린 또는 (R)-레티큘린 전구체의 수준에 대해 조절된다. 세포가 (S)-레티큘린 또는 벤질이소퀴놀린 유도체를 자연적으로 생성할 수 없는 구현예에서, (S)-레티큘린 또는 벤질이소퀴놀린 유도체는 세포의 성장 배지의 일부로서 세포에 제공될 수 있다. 세포가 (S)-레티큘린 또는 벤질이소퀴놀린 유도체를 자연적으로 생성할 수 없는 다른 구현예에서, 세포에 의해 (S)-레티큘린 또는 벤질이소퀴놀린 유도체로 각각 변환될 수 있는 (S)-레티큘린 또는 벤질이소퀴놀린 유도체의 전구체 화합물이 제공될 수 있다. 세포 성장 배지의 일부로서 세포에 제공될 수 있는 대안적인 기질은 (S)-노르코클라우린, (S)-N-메틸노르코클라우린, (S)-노르라우다노살린, (S)-N-메틸노르라우다노살린, (S)-코클라우린, (S)-N-메틸코클라우린, (S)-3'-하이드록시코클라우린, (S)-3'-하이드록시-N-메틸코클라우린, (S)-히게나민, (S)-N-메틸히게나민, (S)-라우다노솔린, (S)-노르레티큘린, (S)-콜레틴, 및 (S)-오리엔타린을 포함하지만, 이들로 한정되는 것은 아니다. 본원에 따라 사용될 수 있는 세포는 박테리아, 효모, 또는 기타 진균 세포, 식물 세포, 동물 세포, 또는 합성 세포를 제한 없이 포함한다.
본 발명은 (S)-광학이성질체를 (R)-광학이성질체로 에피머화하기 위한 조성물을 더 포함하며, 이 조성물은 벤질이소퀴놀린 유도체를 산화 벤질이소퀴놀린 유도체로 산화시킬 수 있는 제1 폴리펩티드 및 산화 벤질이소퀴놀린 유도체를 (R)-레티큘린 전구체로 환원시킬 수 있는 제2 폴리펩티드를 포함하는 효소 혼합물을 포함하며, (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있는 제1 폴리펩티드 및 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 제2 폴리펩티드를 포함하는 효소 혼합물을 더 포함한다. 바람직한 구현예에서, 제1 폴리펩티드는 시토크롬 P450이고, 제2 폴리펩티드는 AKR이다.
일부 구현예에서, AKR 및 CYP450 폴리펩티드는 작동 가능하게 연결되어 융합 폴리펩티드를 형성한다. 따라서, 본 발명은, 서열 번호 323을 포함하거나 서열 번호 323으로 이루어진 폴리펩티드를 더 포함한다.
본 발명은 (S)-광학이성질체를 (R)-광학이성질체로 에피머화할 수 있는 폴리펩티드를 암호화하는 핵산 서열을 포함하는 조성물을 더 포함한다. 바람직한 구현예에서, 핵산 서열은, CYP450 및 AKR을 암호화하는 동시에 (S)-광학이성질체를 (R)-광학이성질체로 에피머화할 수 있는, 바람직하게는 벤질이소퀴놀린 유도체를 산화 벤질이소퀴놀린 유도체로 산화시킬 수 있고 벤질이소퀴놀린 유도체를 (R)-레티큘린 전구체로 환원시킬 수 있는, 더 바람직하게는 (S)-레티큘린을 산화시켜 1,2-디하이드로레티큘린을 형성할 수 있고 1,2-디하이드로레티큘린을 환원시켜 (R)-레티큘린을 형성할 수 있는 핵산 서열이다. 바람직한 구현예에서, AKR 및 CYP450을 암호화하는 핵산 서열은 작동 가능하게 연결되어 CYP450-AKR 융합 폴리펩티드를 생성한다. 따라서, 본 발명은 서열 번호 322를 더 포함한다.
숙주 세포에 축적되는 (R)-레티큘린의 양은 다를 수 있다. 숙주 세포가 (R)-레티큘린 및 (S)-레티큘린을 자연적으로 생성하는(예를 들어, Papaver somniferum 세포) 발명의 구현예에서, 본 발명에 따른 키메라 핵산 서열을 포함하도록 제조된 세포에 의해 생체 내에서 합성된 (R)-레티큘린 대 (S)-레티큘린의 비는 본래 숙주 세포(즉, 키메라 핵산 서열을 포함하지 않은 세포) 또는 숙주 세포 추출물에 존재하는 (R)-레티큘린 대 (S)-레티큘린의 비를 초과한다. 바람직하게, 숙주 세포 또는 숙주 세포 추출물에서 (R)-레티큘린 대 (S)-레티큘린의 비는 21:79보다 크며, 예를 들어, 적어도 0.3:1, 적어도 0.4:1, 적어도 0.5:1, 적어도 1:1, 적어도 2:1, 적어도 3:1, 또는 적어도 4:1이다.
(R) -레티큘린 및 (R) -레티큘린 전구체의 용도
본 발명에 따라 얻은 (R)-레티큘린은 의약품, 치료제 또는 의약제로서의 사용을 위해 제형화될 수 있다. 따라서, 본 발명은 본 발명의 방법에 따라 제조된 (R)-레티큘린을 포함하는 제약 조성물을 더 포함한다. 본 발명에 따른 (R)-레티큘린을 포함하는 의약품 조제물은 바람직하게 부형약, 부형제, 희석제, 보조 물질, 예컨대 습윤제 또는 유화제, pH 완충 물질 등을 더 포함한다. 이러한 부형약, 부형제 및 보조 물질은 일반적으로 과도한 독성 없이 투여될 수 있는 약제이다. 약학적으로 허용 가능한 부형제는 물, 식염수, 폴리에틸렌글리콜, 히알루론산, 글리세롤, 및 에탄올 등의 액체를 포함하지만, 이들로 한정되는 것은 아니다. 약학적으로 허용 가능한 염, 예를 들어 염산염, 인산염, 황산염 등의 무기산 염, 및 아세트산염, 프로피온산염, 벤조산염 등의 유기산의 염이 포함될 수도 있다. 반드시 필요한 것은 아니지만, 조제물이 안정제 역할을 하는 약학적으로 허용 가능한 부형제를 포함하는 것도 바람직하다. 폴리펩티드의 안정제 역할도 하는 적합한 담체는 제약 등급의 덱스트로스, 수크로스, 락토스, 소르비톨, 이노시톨, 덱스트란 등을 제한 없이 포함한다. 기타 적합한 담체는 전분, 셀룰로오스, 인산나트륨 또는 인산칼슘, 시트르산, 글리신, 폴리에틸렌 글리콜(PEG), 및 이들의 조합을 제한 없이 포함한다. 제약 조성물은 경구용, 정맥 투여용, 및 원하는 다른 경로의 투여용으로 제형화될 수 있다. 투여량은 다를 수 있고, 통상적인 실험을 이용하여 최적화될 수 있다. (R)-레티큘린을 포함하는 제약 조성물은 탈모 또는 근육 긴장을 치료하는 데 사용될 수 있다.
다른 구현예에서, 본 발명은 본 발명에 따라 제조된 (R)-레티큘린을 포함하는 제약 조성물로 환자를 치료하는 방법을 제공한다. 따라서, 본 발명은 본 발명의 방법에 따라 제조된 (R)-레티큘린으로 환자를 치료하는 방법으로서, (R)-레티큘린을 포함하는 조성물을 환자에게 투여하는 단계를 포함하되, (R)-레티큘린은 환자의 건강 상태를 개선하기에 충분한 양으로 투여되는, 방법을 더 제공한다. 바람직한 구현예에서, 건강 상태는 탈모 및 근육 긴장의 완화로 이루어진 군으로부터 선택된다.
또한, 본원에 제공된 (R)-레티큘린은, 살루타리딘, 코데인 및 모르핀을 제한 없이 포함하고, 테바인, 파파베린, 노스카핀, 코다민, 라우딘 및 라우다노신 (+)-팔리딘, (-)-이소볼딘, 및 (-)-코리투베린을 더 포함하는 기타 2차 대사 물질 또는 의약 조성물을 제조하기 위한 제제로서 유용하다.
본원에 제공된 (R)-레티큘린 전구체는 기타 2차 대사 물질 특히 (R)-레티큘린을 제조하기 위한 제제로서 유용하다. 도 1에 도시된 바와 같이, (R)-N-메틸코클라우린은 (R)-3'-하이드록시-N-메틸코클라우린을 제조하기 위한 제제로서 사용될 수 있다. (R)-3'-하이드록시-N-메틸코클라우린은 (R)-레티큘린을 제조하기 위한 제제로서 사용될 수 있다.
AKR 및 CYP450 폴리펩티드를 암호화하는 뉴클레오티드 서열의 타용도
다른 양태에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열은 샘플에서 유전자의 존재 유무를 검출하는 데 이용될 수 있다. 따라서, 본 발명의 일 구현예에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열의 존재 유무를 검출하는 방법으로서,
(a) AKR 및/또는 CYP450을 암호화하는 핵산 서열을 포함하는 것으로 의심되는 샘플을 제공하는 단계; 및
(b) AKR 및/또는 CYP450을 암호화하는 뉴클레오티드 서열의 존재에 대해 샘플을 분석하는 단계를 포함하는, 방법이 제공된다.
바람직한 구현예에서, 샘플은 게놈 DNA를 포함하는 세포를 포함한다. 따라서, 바람직한 구현예에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열의 존재 유무를 검출하는 방법으로서,
(a) 세포를 제공하는 단계;
(b) 세포로부터 게놈 DNA를 추출하는 단계; 및
(c) AKR 및/또는 CYP450을 암호화하는 핵산 서열의 존재에 대해 게놈 DNA를 분석하는 단계를 포함하는, 방법이 제공된다.
게놈 DNA를 분석하는 방법은 일반적으로 본 기술분야에 알려져 있으며, 예를 들어 중합효소 연쇄 반응(PCR) 및 AKR 및/또는 CYP450을 암호화하는 뉴클레오티드 서열의 특정 일부를 증폭하기 위한 특정 폴리뉴클레오티드 프라이머의 사용을 포함한다. 또한, 제한효소 분해 및 서던 블롯(Southern blot) 분석이 이용될 수 있다. 분석은 또한, 인트론, 엑손 또는 AKR 및/또는 CYP450을 암호화하는 핵산 서열의 상위 또는 하위 영역에 관한 것일 수 있다. 분석은 또한, AKR 및/또는 CYP450을 암호화하는 핵산 서열을 포함하는 게놈 좌위 식별에 관한 것일 수 있고, 이러한 좌위는 조절된 수준의 AKR 및/또는 CYP450 발현에 연결된다.
바람직한 구현예에서, 세포는 식물 세포이다. 더 바람직한 구현예에서, 세포는 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하는 식물로부터 얻은 식물 세포이며, 더 바람직하게 식물은 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속한다.
바람직한 구현예에서, 상기 분석을 수행하기 위해 사용되는 CYP450 및/또는 AKR 서열은 서열 번호 116 내지 서열 번호 218, 서열 번호 324, 및 서열 번호 337에 명시되어 있거나, 서열 번호 1 내지 서열 번호 58, 서열 번호 326, 서열 번호 328, 및 서열 번호 339에 명시된 서열, 또는 서열 번호 322에 명시된 서열이다.
다른 양태에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열은, 조절된 수준의 AKR 및/또는 CYP450 발현을 갖는 세포를 생성하는 데 사용될 수 있다. 이러한 세포는 바람직하게 AKR 및/또는 CYP450을 천연적으로 발현하는 식물 세포이며, 더 바람직하게는 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하는 식물로부터 얻은 식물세포이며, 가장 바람직하게 식물은 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속한다. 따라서, 본 발명은 AKR 및/또는 CYP450을 자연적으로 발현하는 세포에서 핵산 서열의 발현을 조절하는 방법으로서,
(a) AKR 및/또는 CYP450을 자연적으로 발현하는 세포를 제공하는 단계;
(b) 세포에 돌연변이를 일으키는 단계;
(c) 세포를 성장시켜 복수의 세포를 얻는 단계; 및
(d) 복수의 세포가, 조절된 수준의 AKR 및/또는 CYP450을 포함하는 세포를 포함하는지 결정하는 단계를 포함하는, 방법을 더 제공한다.
바람직한 구현예에서, 이 방법은,
(e) 조절된 수준의 AKR 및/또는 CYP450을 포함하는 세포를 선택하고, 이러한 세포를 성장시켜 복수의 세포를 얻는 단계를 더 포함한다.
더 바람직한 구현예에서, 돌연변이를 유발시키기 위해 식물 종자 세포가 사용된다. 사용될 수 있는 돌연변이 유발제는, 염기 유사체, 탈아미노화제, 알킬화제, 삽입물질, 트랜스포존, 브롬, 아지드화나트륨, 에틸 메탄설포네이트(EMS)를 제한 없이 포함하는 화학 작용제, 뿐만 아니라 이온화 방사선 및 자외선과 같은 방사선을 제한 없이 포함하는 물리 작용제이다. 따라서, 본 발명은 AKR 및/또는 CYP450을 자연적으로 발현하는 세포에서 핵산 서열의 조절된 발현을 포함하는 종자 식물을 생성하는 방법으로서,
(a) AKR 및/또는 CYP450을 자연적으로 발현하는 종자 식물을 제공하는 단계;
(b) 식물의 종자에 돌연변이를 일으켜 돌연변이 종자를 얻는 단계;
(c) 돌연변이 종자를 다음 세대 종자를 뿌릴 수 있는 다음 세대 돌연변이 식물로 성장시키는 단계; 및
(d) 돌연변이 식물의 다음 세대 종자 또는 다른 부분을 얻어서, 다음 세대 식물 또는 다음 세대 종자가, 조절된 AKR 및/또는 CYP450 발현을 나타내는지 분석하는 단계를 포함하는, 방법을 더 제공한다.
바람직한 구현예에서, 복수 세대의 식물 및/또는 종자가 얻어질 수 있으며, 이러한 세대 중 임의의 세대 또는 모든 세대의 식물 및/또는 종자의 일부가 분석될 수 있다. 분석은 일반적으로 비돌연변이(야생형) 식물 또는 종자에서의 발현 수준(예를 들어, RNA 수준 또는 단백질 수준)을 돌연변이 식물 또는 종자에서의 발현과 비교함으로써 수행된다. 더 바람직한 구현예에서, 단계 (d)에서의 분석은 야생형 DNA와 돌연변이 DNA 간의 이형2중가닥 형성을 분석함으로써 수행될 수 있다. 따라서, 바람직한 구현예에서, 단계 (d)에서의 분석은,
i. 돌연변이 식물로부터 DNA를 추출하는 단계;
ii. AKR 및/또는 CYP450을 암호화는 핵산 서열을 포함하는 DNA의 일부를 증폭하여 증폭된 돌연변이 DNA를 얻는 단계;
iii. 야생형 식물로부터 DNA를 추출하는 단계;
iv. 야생형 식물에서 추출한 DNA를 증폭된 돌연변이 DNA와 혼합하여 이형2중가닥 폴리뉴클레오티드를 형성하는 단계;
v. 불일치 이형2중가닥 폴리뉴클레오티드의 영역에서 제한할 수 있는 단일가닥 제한효소와 함께 이형2중가닥 폴리뉴클레오티드를 배양시키는 단계; 및
vi. 이형2중가닥 폴리뉴클레오티드에서 불일치 부위를 결정하는 단계를 포함한다.
바람직한 구현예에서, 사용되는 AKR 및/또는 CYP450을 암호화하는 핵산 서열은 서열 번호 116 내지 서열 번호 218, 서열 번호 324, 및 서열 번호 337에 명시되어 있으며, 서열 번호 1 내지 서열 번호 58, 서열 번호 326, 서열 번호 328, 및 서열 번호 339에 명시된 서열, 또는 서열 번호 322에 명시된 서열이다.
다른 양태에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열은 유전자 침묵에 의해 조절된 수준의 AKR 및/또는 CYP450의 발현을 가진 세포를 생성하는 데 사용될 수 있다. 따라서, 본 발명은 세포에서 AKR 및/또는 CYP450의 발현을 감소시키는 방법으로서,
(a) AKR 및/또는 CYP450을 발현하는 세포를 제공하는 단계; 및
(b) 세포에서 AKR 및/또는 CYP450의 발현을 침묵시키는 단계를 포함하는, 방법을 더 포함한다.
바람직한 구현예에서, 세포는 식물 세포이다. 바람직하게, 식물은 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하는 구성원이며, 식물은 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속한다. 사용되는 AKR 및/또는 CYP450을 침묵시키는 바람직한 방법은 (본 기술분야에서는 VIGS로 알려진) 바이러스 유도 유전자 침묵이다. 일반적으로, 변형되지 않은 바이러스에 감염된 식물에서, 바이러스 게놈을 대상으로 한다. 그러나, 바이러스 벡터가 숙주 유전자 (예를 들어, AKR 및/또는 CYP450을 암호화하는 서열의 일부)로부터 유도된 삽입 유전자를 운반하도록 변형되었을 경우, 공정은 추가적으로 해당 mRNA를 대상으로 한다. 따라서, 본 발명은 감소된 수준의 AKR 및/또는 CYP450을 발현하는 식물을 생성하는 방법으로서,
(a) AKR 및/또는 CYP450을 발현하는 식물을 제공하는 단계; 및
(b) 바이러스 유도 유전자 침묵을 이용하여 식물에서 AKR 및/또는 CYP450의 발현을 감소시키는 단계를 포함하는, 방법을 더 포함한다.
이 양태의 발명은 실시예 5에 더 상세히 설명되어 있다.
AKR 및/또는 CYP450의 발현을 조절하기 위한 전술한 방법은, 모르핀, 코데인, 테바인, 파파베린, 노스카핀, (S)-레티큘린, (R)-레티큘린, 코다민, 라우다닌 및 라우다노신을 제한 없이 포함하는 식물 내 식물 알칼로이드 수준의 조절을 일으킬 수 있다. 방법은 (S)-레티큘린, (R)-레티큘린의 비의 조절을 더 일으킬 수 있다. 바람직하게, 이러한 조절은 식물 세포 또는 식물 세포 추출물에서 (R)-레티큘린 대 (S)-레티큘린의 비가 21:79 미만, 더 바람직하게는 0.1 미만, 더 바람직하게는 0.05 미만, 더 바람직하게는 0.025 미만, 더 바람직하게는 0.01 미만이 되도록 한다. 이러한 조절은 실시예 5에 예시되어 있다. 따라서, 본 발명은 식물 알칼로이드를 자연적으로 생성할 수 있는 식물에서 식물 알칼로이드 생성 수준을 조절하기 위한 방법의 용도를 포함한다. 바람직하게, 이러한 식물은 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하며, 더 바람직하게, 식물은 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속한다.
본 발명의 또 다른 양태에서, AKR 및/또는 CYP450을 암호화하는 핵산 서열은 유전자형 식물에 사용될 수 있다. 바람직하게, 식물은 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하는 구성원이며, 더 바람직하게 식물은 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속한다. 일반적으로, 유전형질 분석은 염색체 쌍의 상동염색체를 구별하는 수단을 제공하며, 식물 개체의 다음 세대에서 분리개체를 식별하는 데 이용될 수 있다. 분자 표지 방법은 계통 발생 연구, 식물 품종들 간의 유전적 관계의 특성화, 교차 또는 체세포 잡종의 식별, 단일 유전자 특성에 영향을 미치는 염색체 단편 국소화, 맵 기반 복제, 및 양적 유전 연구에 이용될 수 있다. 예를 들어, Plant Molecular Biology: A Laboratory Manual, 7장, Clark 편저, Springer-Verlag, Berlin (1997)을 참조할 수 있다. 분자 표지 방법에 대해서는, 일반적으로 Genome Mapping in Plants (Andrew H. Paterson 편저) Academic Press/R. G. Landis Company 출판, Austin, Tex., pp.7-21의 The DNA Revolution, Andrew H. Paterson저, 1996 (Chapter 2)를 참조할 수 있다. 본 발명에 따른 유전형질 분석의 특정 방법은 제한효소 단편 길이 다형성(RFLP)을 제한 없이 포함하는 임의의 분자 표지 분석 기법의 이용을 포함할 수 있다. RFLP는 뉴클레오티드 서열 변이성에 의해 야기되는 DNA 제한효소 단편들 간의 대립유전자 차이를 반영한다. 당업자에게 알려진 바와 같이, RFLP는 일반적으로 식물 게놈 DNA의 추출 및 하나 이상의 제한효소를 이용한 게놈 DNA의 분해에 의해 검출된다. 일반적으로, 생성된 단편은 크기에 따라 분리되고 핵산 프로브로 혼성화된다. 상동염색체로부터 제한효소 단편이 드러난다. 대립유전자 간의 단편 크기 차이가 RFLP를 나타낸다. 따라서, 본 발명은 RFLP 분석과 같은 기법을 이용하여, AKR 및/또는 CYP450을 암호화하는 게놈 DNA의 일부, 뿐만 아니라 이러한 AKR 및/또는 CYP450을 암호화하는 핵산 서열에 유전적으로 연결된 염색체 핵산 서열을 분리하기 위한 수단을 더 제공한다. 연결된 염색체 핵산 서열은 AKR 및/또는 CYP450을 암호화하는 게놈 핵산 서열의 50 센티모건(cM) 이내, 흔히 40 또는 30 cM 이내, 바람직하게는 20 또는 10 cM 이내, 더 바람직하게는 5, 3, 2, 또는 1 cM 이내이다. 따라서, 본 발명에 따르면, 본 발명의 AKR 및/또는 CYP450을 암호화하는 서열은 식물 개체에서 유전적으로 연결된 핵산 서열의 분리를 평가하기 위한 표지로서 사용될 수 있다. 바람직하게, 식물 개체는 양귀비과, 녹나무과, 포포나무과, 대극과 또는 뽕나무과 식물과에 속하는 식물을 포함하거나 그러한 식물로 이루어지며, 더 바람직하게, 식물 개체는 Papaver somniferum, Papaver bracteatum 또는 Papaver rhoeas 종에 속하는 식물을 포함하거나 그러한 식물로 이루어진다.
본 발명에 따르면, 식물 핵 게놈의 분자 표지 매핑에 이용되는 핵산 프로브는, 선택적인 혼성화 조건에서, AKR 및/또는 CYP450을 암호화하는 게놈 서열에 선택적으로 혼성화된다. 바람직한 구현예에서, 프로브는 본 발명에 의해 제공되는 AKR 및/또는 CYP450을 암호화하는 핵산 서열로부터 선택된다. 일반적으로, 이러한 프로브는 cDNA 프로브이다. 일반적으로, 이러한 프로브의 길이는 적어도 15 염기, 더 바람직하게는 적어도 20, 25, 30, 35, 40, 또는 50 염기이다. 그러나, 프로브의 길이는 일반적으로 약 1 킬로염기 미만이다. 바람직하게, 프로브는 반수체 식물 염색체 보체에서 고유 좌위에 혼성화되는 단일 복제 프로브이다. RFLP 매핑에 이용되는 일부 예시적인 제한효소는 EcoRI, EcoRv, 및 SstI이다. 본원에 사용된 용어 "제한효소"는 특정 뉴클레오티드 서열에서 폴리뉴클레오티드를 인지하고, 단독으로 또는 다른 조성물과 함께, 절단하는 조성물에 대한 참조를 포함한다.
1) 단일가닥 형태 분석(SSCP), 2) 변성 구배 겔 전기영동(DGGE), 3) 리보뉴클레아제(RNase) 보호 분석법, 4) 대립유전자 특이 올리고뉴클레오티드(ASO), 5) 뉴클레오티드 불일치를 인지하는 단백질, 예컨대 대장균 mutS 단백질의 사용, 및 6) 대립유전자 특이 PCR을 제한 없이 포함하여 당업자에게 잘 알려진 분자 표지 기법을 활용하여 본 발명의 핵산 서열의 다형성(대립유전자) 변이를 구별하는 다른 방법이 이용될 수 있다. 2개의 상보적 DNA 가닥 간의 불일치 검출에 기반을 둔 다른 접근법은 클램프형 변성 겔 전기영동(CDGE), 이형2중가닥 분석(HA), 및 화학 불일치 절단(CMC)을 제한 없이 포함한다. 따라서, 본 발명은, 엄격한 혼성화 조건에서, AKR 및 CYP450을 암호화하는 핵산을 포함하는 것으로 의심되는 샘플을, 이에 혼성화될 수 있는 핵산 프로브와 접촉시키는 단계를 포함하는, 유전형질 분석 방법을 더 제공한다. 일반적으로, 샘플은 식물 샘플, 바람직하게는 AKR 및/또는 CYP450을 암호화하는 Papaver somniferum 핵산 서열(예를 들어 유전자, mRNA)을 포함하는 것으로 의심되는 샘플이다. 핵산 프로브는, 엄격한 조건에서, 다형성 표지를 포함하는 AKR 및/또는 CYP450를 암호화하는 핵산 서열의 하위서열에 선택적으로 혼성화된다. 다형성 표지 핵산 서열에 대한 핵산 프로브의 선택적 혼성화는 혼성 복합체를 생성한다. 혼성 복합체의 검출은 샘플에 다형성 표지가 존재한다는 것을 나타낸다. 바람직한 구현예에서, 핵산 프로브는 AKR 및/또는 CYP450을 암호화하는 핵산 서열의 일부를 포함한다.
실시예
이하 본 발명의 방법을 수행하기 위한 구체적 실시예와 더불어 본 발명의 조성물을 나타내는 실시예를 제공한다. 실시예는 단지 예시적 목적으로 제공되며, 어떤 식으로든 본 발명의 범위를 제한하고자 하는 것은 아니다.
실시예 1 - (S) -레티큘린에서 (R) -레티큘린으로의 변환
본 실시예는 CYP450 및 AKR 모이어티로 이루어진 Papaver somniferum의 융합 폴리펩티드(서열 번호 323) 형태의 효소 혼합물을 이용하여 (S)-레티큘린이 (R)-레티큘린으로 생체 외 변환되는 것을 설명한다.
pESC-leu2d::PsCPR/PsREPI를 지닌 맥주 효모균(Saccharomyces cerevisiae) 균주 YPH499를 성장시키고 마이크로솜을 이하 설명된 바와 같이 정제하였다. 간략히, 2% 글루코오스로 보충된, 류신 없는 합성 완전(SC) 배지에서, 30℃ 및 250 rpm에서 16시간 동안 효모 균주를 성장시켰다. 1 mL의 배양액을 1.8% 갈락토오스, 0.2% 글루코오스 및 1% 라피노오스로 보충된, 류신 없는 SC 배지 50 mL에 첨가하고, 30℃ 및 250 rpm에서 72시간 동안 성장시켰다. 이후 4,000 g에서 5분 동안 배양액을 원심분리하고, 5 mL의 TEK 완충액(50 mM Tris-HCl pH8, 1mM EDTA, 100 mM KCl)으로 세정하였다. 펠릿을 1 mL TESB 완충액(50 mM Tris-HCl pH 8, 1 mM EDTA, 0.6 M 소르비톨)에 재부유시키고, 동일한 부피의 0.5 mm 글래스 비드를 첨가하였다. 시험관을 10℃에서 4분 동안 손으로 흔들었다. TESB로 비드를 세정하고 세정액을 모아 14,000 g에서 10분 동안 원심분리하였다. 상층액을 125,000 g에서 1 시간 동안 초원심분리하고, 상층액을 버렸다. 이후 마이크로솜을 pH 7.5의 50 mM HEPES에 재부유시켰다.
효소 분석물은 HEPES 완충액(pH 7.5)에 500 μM NADPH 및 50 μM의 (S)-레티큘린을 함유하였고, 마이크로솜을 전술한 바와 같이 제조하였다. 분석물을 30℃에서 밤새 배양하였다. 반응 후, Agilent 1260 HLPC에 분석 샘플을 0.2 ml/분의 유량으로 통과시키고, LUX 셀룰로오스-1 키랄 컬럼(150 mm × 2.1 mm 내경, Phenomenex)을 이용하여 0.1% 디에틸아민으로 보충된 75% 중탄산 암모늄(용매 A) 및 0.1% 디에틸아민으로 보충된 25% 아세토니트릴(용매 B)로 화합물을 분리하였다. 284 nm의 파장에서 (R)-레티큘린 및 (S)-레티큘린을 모니터링하였다.
결과를 도 3에 나타내었다. 도 3에서 알 수 있는 바와 같이, 신뢰할 수 있는 표준 대조군 (R)-레티큘린 및 (S)-레티큘린의 키랄 컬럼에서의 체류 시간은 각각 약 13.5분(맨 위 그림) 및 15분(위에서 두 번째 그림)이다. 맨 아래 그림은 혼합물에 효소가 존재하지 않는 분석물의 결과를 나타내며 이러한 반응 조건에서 (S)-레티큘린은 (R)-레티큘린으로 에피머화되지 않음을 보여준다. 위에서 세 번째 그림은, 효소 혼합물의 존재 시 (S)-레티큘린이 (R)-레티큘린으로 에피머화되는 것을 나타낸다(화살표 참조, 약 15분의 체류 시간에서 피크의 등장).
실시예 2 - (S) -레티큘린에서 1,2-디하이드로레티큘린으로의 변환
본 실시예는 Papaver rhoeas의 CYP450(서열 번호 325)을 이용하여 효모에서 (S)-레티큘린이 1,2-디하이드로레티큘린으로 생체 외 변환되는 것을 설명한다. pESC-leu2d::PsCPR/PrDRS를 지닌 맥주 효모균 균주 YPH499를 성장시키고 마이크로솜을 이하 설명된 바와 같이 정제하였다. 간략히, 2% 글루코오스로 보충된, 류신 없는 합성 완전(SC) 배지에서, 30℃ 및 250 rpm에서 16시간 동안 효모 균주를 성장시켰다. 이 배양액 1 mL를 1.8% 갈락토오스, 0.2% 글루코오스 및 1% 라피노오스로 보충된, 류신 없는 SC 배지 50 mL에 첨가하고, 30℃ 및 250 rpm에서 72시간 동안 성장시켰다. 이후 4,000 g에서 5분 동안 배양액을 원심분리하고, 5 mL의 TEK 완충액(50 mM Tris-HCl pH8, 1mM EDTA, 100 mM KCl)으로 세정하였다. 이후 펠릿을 1 mL TESB 완충액(50 mM Tris-HCl pH 8, 1 mM EDTA, 0.6 M 소르비톨)에 재부유시키고, 동일한 부피의 0.5 mm 글래스 비드를 첨가하였다. 시험관을 10℃에서 4분 동안 손으로 흔들었다. TESB로 비드를 세정하고 세정액을 모아 14,000 g에서 10분 동안 원심분리하였다. 이후 상층액을 125,000 g에서 1 시간 동안 초원심분리하고, 상층액을 버렸다. 이후 마이크로솜을 pH 7.5의 50 mM HEPES에 재부유시켰다.
효소 분석물은 HEPES 완충액(pH 7.5)에 500 μM NADPH 및 50 μM의 (S)-레티큘린을 함유하였고, 마이크로솜을 전술한 바와 같이 제조하였다. 분석물을 30℃에서 밤새 배양하였다. 반응 후, 양이온 모드에서 작동하는 전자분무 이온화 소스를 가진 6400 B 질량 분석기에 연결된 Agilent 1260 HLPC에 분석 샘플을 통과시켰다. 질량 분석기는 200 내지 400 m/z를 스캔하였다. HLPC 방법을 이용하여 앞서 설명한 효소 분석물에 대해 화합물을 분리하였다(Farrow SC and Facchini, P], (2013), J. Biol. Chem. (288) pp 28,997-29,012; dioxygenases catalyze O-demethylation and 0,0-demethylation with widespread roles in benzylisoquinoline alkaloid metabolism in opium poppy).
결과를 도 4에 나타내었다. 도 4(맨 위 그림)에서 알 수 있는 바와 같이, 효소를 포함하지 않은 대조군 샘플에서 약 3.1분의 체류 시간을 갖는 피크가 HPLC 컬럼 상에서 발견된다. 이 피크는 m/z 330에서 (S)-레티큘린에 대한 충돌 유도 해리 스펙트럼의 가장 큰 단편에 있어서 3.13분의 예측된 체류 시간과 부합한다(표 1 참조). 위에서 두 번째 그림은 동일한 대조군 샘플의 m/z 328에서 피크가 발견되지 않음을 보여주므로, 대조군 샘플에 1,2-디하이드로레티큘린이 존재하지 않는다는 것을 나타낸다. 위에서 세 번째 그림은 효소를 포함하는 샘플의 m/z 330에서 약 3.1분의 체류 시간에서 피크가 관찰되는 것을 보여주므로, 분석 샘플에 (S)-레티큘린이 존재한다는 것을 나타낸다. 맨 아래 그림은 분석 샘플의 m/z 328에서 약 3.0의 체류 시간을 갖는 피크가 관찰되는 것을 보여준다. 이 피크는 m/z 328에서 1,2-디하이드로레티큘린에 대한 충돌 유도 해리 스펙트럼의 가장 큰 단편의 3.02분의 예측된 체류 시간과 부합하며(표 1 참조), 효소의 존재 시 분석 샘플에 1,2-디하이드로레티큘린이 존재한다는 것을 나타낸다.
실시예 3 - 1,2-디하이드로레티큘린에서 (R) -레티큘린으로의 변환
본 실시예는 Papaver rhoeas의 AKR(서열 번호 327)을 이용하여 효모에서 1,2-디하이드로레티큘린이 (R)-레티큘린으로 생체 외 변환되는 것을 설명한다.
50 ㎍/mL 황산 카나마이신과 pET47b::PrDRR을 지닌 대장균 균주 로제타(DE3)의 클로람페니콜 배양액 35 ㎍/mL로 보충된 50 mL LB를 1 L의 동일한 배지에 첨가하고 0.6의 OD600까지 37℃, 180 rpm에서 16시간 동안 성장시켰다. 이후 IPTG를 1 mM의 최종 농도에 첨가하고 25℃, 180 rpm에서 4시간 동안 성장하도록 하고, 원심분리에 의해 세포 펠릿을 회수하였다. 세포를 완충액 A(100 mM 인산나트륨 완충액 pH 7.0, 300 mM NaCl, 10% (v/v) 글리세롤)에 용해시키고 프렌치 프레스를 이용하여 2 mM 페닐메탄설포닐플루오라이드(PMSF)로 보충하였다. 14,000 g에서 15분 동안 원심분리하여 세포 파편을 제거하였다. 총 가용성 단백질 추출물을 4℃, 65 rpm에서 45분 동안 완충액 A-평형 TALON(Clone tech) 수지와 화합시켰다. 수지를 완충액 A로 2번 세정하고, 완충액 A 내 이미다졸(2.5, 10, 100, 200 mM)의 구배를 이용하여 단계적으로 단백질을 용출시켰다. 정제된 단백질을 100 mM 이미다졸에서 용출시켰다.
효소 분석물은 인산나트륨 완충액(pH 7.0)에 500 μM NADPH 및 50 μM의 1,2-디하이드로레티큘린을 함유하였고, 단백질을 전술한 바와 같이 제조하였다. 분석물을 30℃에서 밤새 방치하였다. 반응 후, 양이온 모드에서 작동하는 전자분무 이온화 소스를 가진 6400 B 질량 분석기에 연결된 Agilent 1260 HLPC에 분석 샘플을 통과시켰다. 질량 분석기는 200 내지 400 m/z를 스캔하였다. HLPC 방법을 이용하여 앞서 설명한 효소 분석물에 대해 화합물을 분리하였다(Farrow SC and Facchini, P], (2013), J. Biol. Chem. (288) pp 28,997-29,012; dioxygenases catalyze O-demethylation and 0,0-demethylation with widespread roles in benzylisoquinoline alkaloid metabolism in opium poppy).
결과를 도 5에 나타내었다. 도 5(맨 위 그림)에서 알 수 있는 바와 같이, 효소를 포함하지 않은 대조군 샘플에서 약 3.0분의 체류 시간을 갖는 피크가 HPLC 컬럼 상에서 발견된다. 이 피크는 m/z 328에서 1,2-디하이드로레티큘린에 대한 충돌 유도 해리 스펙트럼의 가장 큰 단편에 있어서 3.02분의 예측된 체류 시간과 부합한다(표 1 참조). 위에서 두 번째 그림은 약 3.1분의 체류 시간에서 피크가 발견되지 않음을 보여주므로, 샘플에 (R)-레티큘린이 존재하지 않는다는 것을 나타낸다. 동일한 대조군 샘플에서 약 3.0분에 작은 피크가 발견된다. 이 피크는 기질 1,2-디하이드로레티큘린의 동위원소 형태를 나타낸다. 위에서 세 번째 그림은 효소를 포함하는 샘플의 m/z 328에서 약 3.0분의 체류 시간을 갖는 피크가 관찰되는 것을 보여주므로, 분석 샘플에 소량의 소모되지 않은 1,2-디하이드로레티큘린이 존재한다는 것을 나타낸다. 맨 아래 그림은 분석 샘플의 m/z 330에서 약 3.1의 체류 시간을 갖는 피크가 관찰되는 것을 보여준다. 이 피크는 m/z 330에서 (R)-레티큘린에 대한 충돌 유도 해리 스펙트럼의 가장 큰 단편의 3.13분의 예측된 체류 시간과 부합하며(표 1 참조), 효소의 존재 시 분석 샘플에 (R)-레티큘린이 존재한다는 것을 나타낸다.
실시예 4 - (S)-N -메틸코클라우린에서 (R)-N -메틸코클라우린으로의 변환
본 실시예는 CYP450 및 AKR 모이어티로 이루어진 Papaver somniferum의 융합 폴리펩티드(서열 번호 2) 형태의 효소 혼합물을 이용하여 효모에서 (S)-N-메틸코클라우린이 (R)-N-메틸코클라우린으로 생체 외 변환되는 것을 설명한다.
pESC-leu2d::PsCPR/PsREPI를 지닌 맥주 효모균 균주 YPH499를 성장시키고 마이크로솜을 이하 설명된 바와 같이 정제하였다. 간략히, 2% 글루코오스로 보충된, 류신 없는 합성 완전(SC) 배지에서, 30℃ 및 250 rpm에서 16시간 동안 효모 균주를 성장시켰다. 1 mL의 배양액을 1.8% 갈락토오스, 0.2% 글루코오스 및 1% 라피노오스로 보충된, 류신 없는 SC 배지 50 mL에 첨가하고, 30℃ 및 250 rpm에서 72시간 동안 성장시켰다. 이후 4,000 g에서 5분 동안 배양액을 원심분리하고, 5 mL의 TEK 완충액(50 mM Tris-HCl pH8, 1mM EDTA, 100 mM KCl)으로 세정하였다. 펠릿을 1 mL TESB 완충액(50 mM Tris-HCl pH 8, 1 mM EDTA, 0.6 M 소르비톨)에 재부유시키고, 동일한 부피의 0.5 mm 글래스 비드를 첨가하였다. 시험관을 10℃에서 4분 동안 손으로 흔들었다. TESB로 비드를 세정하고 세정액을 모아 14,000 g에서 10분 동안 원심분리하였다. 상층액을 125,000 g에서 1 시간 동안 초원심분리하고, 상층액을 버렸다. 이후 마이크로솜을 pH 7.5의 50 mM HEPES에 재부유시켰다.
효소 분석물은 HEPES 완충액(pH 7.5)에 500 μM NADPH 및 50 μM의 (S)-N-메틸코클라우린을 함유하였고, 마이크로솜을 전술한 바와 같이 제조하였다. 분석물을 30℃에서 밤새 배양하였다. 반응 후, Agilent 1260 HLPC에 분석 샘플을 0.2 ml/분의 유량으로 통과시키고, LUX 셀룰로오스-1 키랄 컬럼(150 mm × 2.1 mm 내경, Phenomenex)을 이용하여 0.1% 디에틸아민으로 보충된 75% 중탄산 암모늄(용매 A) 및 0.1% 디에틸아민으로 보충된 25% 아세토니트릴(용매 B)로 화합물을 분리하였다. 230 nm의 파장에서 (R)- 및 (S)-N-메틸코클라우린을 모니터링하였다.
결과를 도 6에 나타내었다. 도 6에서 알 수 있는 바와 같이, 신뢰할 수 있는 표준 대조군 (S)-N-메틸코클라우린의 키랄 컬럼에서의 체류 시간은 약 13.9분(맨 위 그림)이다. 맨 아래 그림은 혼합물에 효소가 존재하지 않는 분석물의 결과를 나타내며 이러한 반응 조건에서 (S)-N-메틸코클라우린은 (R)-N-메틸코클라우린으로 에피머화되지 않음을 보여준다. 가운데 그림은, 효소 혼합물의 존재 시 (S)-N-메틸코클라우린이 (R)-N-메틸코클라우린으로 에피머화되는 것을 나타낸다(화살표 참조, 약 16.3분의 체류 시간에서 피크의 등장).
실시예 5 - AKR 및 AKR-CYP450 융합 유전자의 유전자 침묵
본 실시예는 바이러스 유도 유전자 침묵(VIGS)을 이용한 AKR 및/또는 CYP450을 암호화하는 유전자의 침묵을 나타낸다.
양귀비(Papaver somniferum)의 Bea 선택 화학형에서 (서열 번호 322 및 서열 번호 328에 의해 각각 전사되는) 양귀비(Papaver somniferum)로부터의 (코데이논 환원효소를 암호화하는) REPI 및/또는 COR1.3 전사 수준을 담배 얼룩 바이러스(TRV) 벡터 시스템을 이용하여 억제하였다. REPI cDNA의 두 영역(REPI-a(도 7a, 그림 A) 및 REPI-5'(도 7c, 그림 A))과 COR1.3 cDNA의 한 영역(도 7b, 그림 A)을 다음 프라이머 쌍들을 이용하여 증폭하였다.
pTRV2-COR1.3
COR1.3-F, ggatccCATCAGTTCCATGCTCTGGT
COR1.3-R, ggtaccGGGCTCATCTCCACTTGATT
pTRV2-REPI-a
REPI-a-F, ggatccCATCACTTCCAAGCTCTGGT
REPI-a-R, ggtaccGGGCTCATCTCCACTTGAT
pTRV2-REPI-5'
REPI-5'-F, gaattcCCTACATACTGTATTGGGTTGAATCATG
REPI-5'-R, ggtaccTAACGGGATAGGACGGTTT
REPI-a 영역 및 COR1.3 영역은 각각의 경우에 REPI 및 COR1.3의 상호 공동 침묵을 유발하는 상당한 유사성을 나타낸다. 반면, REPI-5' 영역은 유일하며 REPI의 침묵만을 유발할 뿐 COR1.3의 침묵을 유발하지는 않는다.
전술한 바와 같이, 증폭제들을 개별적으로 pTRV2로 복제하고 벡터들을 근두암종균에서 기동시켰다. pTRV1을 지닌 근두암종균과 유전자 특이 단편을 함유한 구조체 pTRV2의 1:1 혼합물을 2 내지 3주 지난 모종의 정단 분열조직에 침투시켰다. 빈 pTRV2를 음성 대조군으로 사용하고, 피토엔 불포화효소를 암호화하는 pTRV2-PDS 구조체를 양성 침투 대조군으로 사용하였다. 침투된 식물을 온실에서 8 내지 10주 동안 재배하였다. 근두암종균의 침투, 및 알칼로이드 및 전사 분석을 위한 유액, 줄기 및 뿌리 샘플의 회수 및 처리를 전술한 바와 같이 수행하였다. 일반적으로, pTRV1을 지닌 근두암종균 및 하나의 pTRV2 구조체를 20 내지 30개의 식물에 침투시켰다. 침투된 식물의 약 70 내지 80%에서, pTRV2 구조체의 기동 단편이 RT-PCR에 의해 검출되었고(도 7a(그림 B); 도 7b(그림 B); 도 7c, 그림 B), 이는 이 식물들이 성공적으로 감염되었음을 나타낸다. 메탄올을 사용하여, 동결 건조된 유액으로부터 알칼로이드를 추출하였다. qRT-PCR로 상대적 전사 존재비를 결정하였다(도 7a(그림 C); 도 7b(그림 C); 도 7c, 그림 C). 6개의 개별적으로 침투된 식물로부터 알칼로이드 함량 및 상대적 전사 존재비 데이터를 생성하였고, 각 샘플에 대해 3개의 기술적 복제를 수행하였다. 침투된 식물에 대한 유액 샘플을 LC-MS/MS로 분석하였다. 총 이온 크로마토그램을 이용하여(도 7a(그림 D); 도 7b(그림 D); 도 7c, 그림 D), 그리고 유액 및 뿌리에서 9개의 상이한 알칼로이드(모르핀, 코데인, 테바인, 파파베린, 노스카핀, 코다민, 라우딘 및 라우다노신)의 상대 존재비를 결정함으로써(도 7a(그림 E); 도 7b(그림 E); 도 7c, 그림 E), pTRV1을 지닌 근두암종균 및 별도의 pTRV2 구조체에서의 REPI 또는 COR1.3의 두 영역 각각이 침투된 양귀비 식물의 알칼로이드 함량에 미치는 영향을 평가하였다. 더 낮은 모르핀 함량뿐만 아니라, REPI 또는 COR1.3의 침묵은 코데인 및 테바인 수준의 상당한 감소를 야기하였다. COR1.3(즉, AKR 유전자)의 침묵뿐만 아니라 REPI(즉, AKR-CYP450 유전자)의 침묵은 레티큘린, 코다민, 라우다닌, 라우다노신 축적의 상당한 증가, 및 덜 일관적으로 파파베린 및 노스카핀 축적의 상당한 증가를 유발하였다. 대조군(pTRV2) 식물의 유액에서 (R)-레티큘린 대 (S)-레티큘린의 비는 약 21:79이었지만, (R)-레티큘린 대 (S)-레티큘린의 비는 pTRV2-REPI-a 식물(도 7a (그림 F)) 및 pTRV2-COR1.3 식물(도 7b (그림 F))의 유액에서 약 2:98로, pTRV2-REPI-5' 식물(도 7c (그림 F))의 유액 및 뿌리에서 약 5:95로 감소하였다.
실시예 6 - NADPH/NADH 및 NADP + /NAD + 의 존재 시 AKR의 촉매 활성
본 실시예는 환원제 NADH 또는 NADPH의 존재 시 AKR 폴리펩티드가 촉매작용을 하는 1,2-디하이드로레티큘린에서 (R)-레티큘린으로의 변환을 나타낸다. 본 실시예는 또한 산화제 NAD+ 또는 NADP+의 존재 시 상기 반응의 가역성을 나타낸다.
Papaver somniferum 및 Papaver rhoeas로부터 얻은 AKR을 사용하여 반응을 수행하였고, 역반응에서 기질로서 (R)-레티큘린이 제공되었고, NAD+ 또는 NADP+를 산화제로 사용하여 효소 반응을 수행한 것을 제외하고, 기본적으로 상기 실시예 3에 설명한 바와 같이 실험을 수행하였다. 마지막에 언급한 반응은 pH 9에서 수행하였다. 도 8에 도시된 바와 같이, NADH 및 NADPH가 모두 존재할 때, Papaver somniferum(PsDRR)(도 8a, 그림 A)과 Papaver rhoeas(PrDRR)(도 8b, 그림 A) 모두로부터의 AKR 폴리펩티드의 촉매량을 사용하여, 1,2-디하이드로레티큘린은 (R)-레티큘린으로 변환된다. 도 8에 더 도시된 바와 같이, Papaver somniferum AKR 폴리펩티드 PsDRR(도 8a, 그림 B)과 Papaver rhoeas 폴리펩티드 PrDRR(도 8b, 그림 B)을 모두 사용하면 반응이 역으로 진행될 수 있으며, NAD+ 또는 NADP+의 존재 시 (R)-레티큘린은 1,2-디하이드로레티큘린으로 변환된다.
실시예 7 - AKR 활성의 pH 의존성
본 실시예는 환원제와 산화제가 모두 존재할 때 AKR 폴리펩티드의 pH 의존성을 나타낸다.
Papaver somniferum과 Papaver rhoeas의 CYP450 및 AKR 폴리펩티드 모두의 pH 의존성을 조사하였다. 각 반응에서 pH를 pH 3.5에서 pH 10까지 점진적으로 증가시킨 것을 제외하고 기본적으로 실시예 3과 실시예 6에 설명한 바와 같이 효소 반응을 수행하였다. 양이온 모드에서 작동하는 전자분무 이온화 소스를 가진 6400 B 질량 분석기에 연결된 Agilent 1260 HLPC에서 샘플을 분석하여 각각의 평가된 pH에서의 효소 활성을 정량화하였다. 질량 분석기는 200 내지 400 m/z를 스캔하였다. HLPC 방법을 이용하여 앞서 설명한 효소 분석물에 대해 화합물을 분리하였다(Farrow SC and Facchini, P], (2013), J. Biol. Chem. (288) pp 28,997-29,012; dioxygenases catalyze O-demethylation and 0,0-demethylation with widespread roles in benzylisoquinoline alkaloid metabolism in opium poppy).
결과를 도 9에 제공하였다. NADPH의 존재 시(PsDRS 정반응) 및 NADP+의 존재 시(PsDRS 역반응), Papaver somniferum CYP450(PsDRS) 및 AKR을 사용하여 효소 활성을 pH의 함수로 나타낸 그래프를 그림 A에 나타내었다. NADPH의 존재 시(PrDRS 정반응) 및 NADP+의 존재 시(PrDRS 역반응), Papaver rhoeas CYP450(PrDRS) 및 AKR을 사용하여 효소 활성을 pH의 함수로 나타낸 그래프를 그림 B에 나타내었다. 도 9에서 알 수 있는 바와 같이, PsDRS 및 PrDRS는 약 pH 8의 최적조건에서 (S)-레티큘린을 1,2-디하이드로레티큘린으로 변환시킨다. NADPH의 존재 시, PsDRR 및 PrDRR은 약 pH 7의 최적조건에서 1,2-디하이드로레티큘린을 (R)-레티큘린으로 변환시킨다. NADP+의 존재 시, PsDRR 및 PrDRR은 약 pH 9의 최적조건에서 (R)-레티큘린을 1,2-디하이드로레티큘린으로 변환시킨다.
실시예 8 - AKR 및 AKR-CYP450 융합 유전자의 유전자 침묵
본 실시예는 바이러스 유도 유전자 침묵(VIGS)을 이용하여 AKR 및/또는 CYP450을 암호화하는 유전자의 추가 침묵을 나타낸다.
REPIa, REPIb 및 COR.1.3 구조체를 사용하여 COR(AKR) 및 REPI(CYP450) 유전자를 표적으로 한 것을 제외하고, 기본적으로 실시예 5에 설명된 바와 같이 유전자 침묵 실험을 수행하였다. REPIa는 COR 유전자와 REPI 유전자 모두에서 보존된 서열을 표적으로 하는 구조체를 나타낸다. 반면, REPIb는 REPI에 고유한 영역을 표적으로 한다. COR1.3은 COR에 고유한 영역을 표적으로 한다. 실시예 5에 설명한 바와 같이 REPI 및 COR의 전사 수준을 결정하였다. 대조군(PTRV2)으로 빈 벡터를 사용하였다. 도 10에서 알 수 있는 바와 같이, REPIb를 통해 고유하게 REPI를 표적으로 하는 식물은 대조군에 비해 감소된 REPI 전사 수준을 나타내는(도 10 - 위 그림) 반면, COR 전사 수준은 실질적으로 동일하게 유지된다(도 10 - 아래 그림). REPIa를 통해 REPI와 COR 모두를 표적으로 하는 식물은 REPI(도 10 - 위 그림) 및 COR(도 10 - 아래 그림)의 감소된 전사 수준을 나타낸다. COR1.3을 사용하여 COR을 표적으로 한 경우, COR 전사 수준은 감소하였다(도 10 - 아래 그림). 또한, COR1.3에 의한 COR 전사 수준의 침묵에 대응하여 REPI 전사 수준도 대조군에 비해 감소하였다(도 10 - 위 그림).
화합물
(HPLC 컬럼)		 체류 시간(분) 충돌 유도 해리 스펙트럼 충돌 에너지 (eV) μmax (nm)
(R)-레티큘린
(키랄 컬럼)		 13.5 없음 없음 284
(S)-레티큘린
(키랄 컬럼)		 15.0 없음 없음 284
(S)-레티큘린
(C18 컬럼)		 3.13 330.2 (10), 210.1 (6), 192.1 (100)
177.1 (4), 175.1 (14), 151.2 (4)
143.1 (16), 137.1 (38)		 25 없음
(R)-레티큘린
(C18 컬럼)		 3.13 330.1 (30), 210.1 (31), 192.1 (100)
175.1 (16), 142.9 (17), 136.9 (28)		 25 없음
디하이드로레티큘린
(C18 컬럼)		 3.02 328.3 (100), 313.2 (83), 312.2 (80)
296.4 (6), 284.2 (26), 252.1 (5)
190.2 (4), 162.4 (7)		 25 없음
SEQUENCE LISTING <110> EPIMERON INC. <120> COMPOSITIONS AND METHODS FOR MAKING (R)-RETICULINE AND PRECURSORS THEREOF <130> IPA160566-US <140> PCT/CA2014/051164 <141> 2014-12-03 <150> US 61/911,759 <151> 2013-12-04 <150> US 62/050,399 <151> 2014-09-15 <160> 340 <170> PatentIn version 3.5 <210> 1 <211> 966 <212> DNA <213> Papaver somniferum <400> 1 atggagagta atggtgtacc tatgatcact ctcagttccg gcattcggat gcctgcttta 60 ggtatgggaa cagctgaaac aatggtaaaa ggaacagaaa gagagaaatt ggcgtttttg 120 aaagcgatag aggtcggtta cagacacttc gatacagctg ctgcatacca aactgaagag 180 tgtcttggtg aagctatagc tgaagcactt caacttggtc taataaaatc tcgagatgaa 240 ctcttcatca cttccaagct ctggtgcgct gatgctcacg ctgatcttgt cctccctgct 300 cttcagaatt ctctgaggaa tcttaaattg gactatcttg atctatattt gatacaccat 360 ccggtaagct tgaagccagg gaagtttgtt aacgaaatac caaaggatca tatccttcca 420 atggactaca aatctgtatg ggcagccatg gaagagtgtc agacccttgg cttcactagg 480 gcaatcgggg tctgtaattt ctcatgcaaa aggcttcaag agttgatgga aacagccaac 540 agccctccag ttgtgaatca agtggagatg agcccgactt tacatcaaaa aaatctgagg 600 gaatattgca aggccaataa tatcatgatc accgcacact cagttttggg agccgtaggt 660 gccgcctggg gcaccaatgc agttatgcat tctaaggtgc ttcaccagat tgctgtggcc 720 agaggaaaat ctgttgccca ggttagtatg agatgggttt accagcaagg cgcgagtctt 780 gtggtgaaaa gtttcaatga agcgaggatg aaggaaaacc ttaagatatt tgattgggaa 840 ctaacggcag aagacatgga aaagatcagt gagattccac aatctagaac aagctctgct 900 gctttcttgt tatcaccgac tggacctttc aaaactgaag aagagttctg ggatgagaag 960 gattga 966 <210> 2 <211> 978 <212> DNA <213> Argmenone mexicana <400> 2 atggaggaag ttattggctt ggaaactgtg gttccaaaag taaccctaag tagtagttca 60 ggtgacaatt ccatgccagt aataggtttt gggacagctg catctccaat ggctgatcat 120 gaaattacga aagctgctgt tctcgttggg atcgaaaatg gttatcgtca cttcgatact 180 gccgctgctt atggaacgga aaaggctgtt ggtgaagcca tagctgaagc cctacgtctc 240 ggtttgatca aatctcgagc tgaagttttt attactacta aactttggtg tagaagttgc 300 gaacgtgacc ttgttgtccc ctcactcaag aatagcctcc aggatttaca aacggattac 360 gtagatcttt tcctcataca ttggccagtg agactacgtc atgacgcagg acgacctcct 420 atttcgagag accaaatcct aacttttgat acaaaatcag tttgggaagg aatggaagaa 480 tgtcatgaat tagggctcgc aaaaaatatc ggagttagta acttttctat caagaagctc 540 gaagaattac tagccacggc aaagattccc ccggtgctcg atcaagtgga actgagccca 600 acttggcacc agaagaatct gatcgagtat tgtaaggaaa agggtattca tgtaacggct 660 tattcagctt taggagccaa taacacacat tggggaaaca atcgagtcgt ggagagtgat 720 gtgttaggtg agatagctaa ggctcgggga aaaactacag ctcagatcgc attgagatgg 780 gtatatgagc aaggtgtgag tatggtggtc aagagtttta accaagaaag gatgaaacaa 840 aaccttcaga tcttcgattt tgagttaact gaagaagagt caaataaaat cagtcaattt 900 ccacagcaca aaggagtaag cctgtctgca gtttatggtg atcatgatat actcaaggag 960 atggaagctg aactttga 978 <210> 3 <211> 969 <212> DNA <213> Argmenone mexicana <400> 3 atggagaatg taattcctag agtgacattg agctctggaa gtgtgatgcc tatattaggt 60 atgggtacag catcataccc atttgttagt tcagaagaag ggaaattggc aatactgaat 120 gcaatcaaag taggttatag acactttgat acagcttcta cttacatgtt tgaggagttt 180 cttggtgaag ctatagctga agcaattcaa cttggactga tcaaatcccg taaagaactg 240 ttcattactt ccaaattatg gtgctgcgat gctcatcctg atcttgtcgt tccttctctt 300 cgaaactcat tacggaatct taagttggag taccttgatt tatatctgat acactttcca 360 gtaagcttga aggcagggag gtatgagtat ccacccctaa aggaagcttt agttctgatg 420 gattacaagt ctgtatgggc agccatggaa gagtgccaaa atcttggcct taccaagtca 480 attggtgtaa gcaacttctc ttgcaagaag cttcaaacta ttttggacta tgccaaaatc 540 cctccctcag ttaatcaagt ggagatgaat ccagtttggc aacagaagaa actgagagaa 600 ttttgcaagg ccaacgatat tgttatcact gcttactcac ctttaggagc tagtggaacc 660 ccgtggggat cgagcagagt acttgatgca caagttctgc atgaaattgc tagggctaaa 720 gggaaaactc atgctcaggt ttgtttgaga tgggtatacg aacaaggagt gagtttgctt 780 gtaaagacct tcaatgaaga gaggatgaag gaaaacctaa ggatatttga ctgggaactg 840 actgaagaag ataagcaaaa gatcagtaaa ataccacagc atagaggact gactggtgat 900 gcttttgtct cagaacatga aggggcaccc ttcaagactg cagaagagtt atgggatgga 960 gaagtttga 969 <210> 4 <211> 969 <212> DNA <213> Argmenone mexicana <400> 4 atggatatga ataaacatgc ggttgtttat gttccaacaa aagtcatcat ggggattcca 60 gtgattggct ttggtacatc agctgatccc cctgtcgact ttgatacgac taggctggca 120 attatgcaag ctattgaaaa tggttacagg cattttgata cagctgctct ttataactcg 180 gaacagcctc ttggtgaagc tattaacgaa gccattagtc gtggattgat taaatctcga 240 gatgaacttt tcatcacttc caaattatgg tgcagcgata cccatccaca acatattctt 300 cctgccatac aaaagactct cagaaatctg aatatggagt atcttgatct gtatcttatc 360 cactggccag tgagttcgag gcacgaaaat tttgaatatc cgataaagaa agaagatttt 420 cttcctatgg attttaagaa agtgtgggcc gcaatggaag aatgtcataa acaggggctc 480 gcaaagttta ttggagtcag caatttctct tgcaagaaac ttgacaatat catggcctct 540 gcaacaattt ccccttcagt tcttcaagtg gaagtaaatc catgttggca acaaaaaagg 600 ttgatagatt tttgcaaagc taatggaata tttgtagttg cttatgctcc tttaggagca 660 gtggggacaa tttatggaac taatagagtt atggaatctg atgttctcaa gcaaattgcc 720 aagaaaagag gaaaaagtgt tgcacaggtt tgtttgagat gggcatatga gcaaggcatt 780 ggtgtggtgg tgaaaagctt taacaaggag aggatgaagc agaaccttga aatattcgat 840 tggaaactga gtgaagagga tacgaagaaa attagtgaaa ttcaacaaga tcgagcttgt 900 cttggaatgg attatacatc accatatgga ccttacaaga caataaaaca actatgggat 960 gaagagtaa 969 <210> 5 <211> 969 <212> DNA <213> Berberis thunbergii <400> 5 atggaaattg ttcctaaagt gacattgaat tctggctatc aaatgcctct tttaggcttc 60 ggaacagccg aaaattggcc gtttaccaaa tctgaagcag ccaaacgggc aatactgtgt 120 gcaatcaaga atggctacag acactttgac acagcttcca tgtaccaatc agaggagtca 180 cttggtgaag ctatagtcga agcgcttcac cttggcctta tcaaatctcg gaatgaactc 240 ttcatcactt ccaagctatg gtgtagtgat tgtcaccaag atcgcgttct ccctgccctt 300 agggagactc tcaagaatct tcacttggat taccttgatc tttacctcat acattggccg 360 ctaagcgcaa agccagggaa gcatgagtac ccaatactaa aagataagct tcttcctatg 420 gactatgaat ccgtatgggg agcaatggaa gaatgccaga ggcttggcct ctctaagtcc 480 attggagtca gcaacttctc tagcaagaag ctggaacagc tactcaaaac tgcaaagatt 540 attcctgcag ttaatgaagt ggaagtgaac cctgtttggc atcaaaataa gctaatagag 600 ttctgtaagg ccaaaggtat tgttgtgact ggttactctc ctttaggtgc caaaggaaca 660 acttggggaa ccaataaagt catggacagt gaggtcctga aagagattgc taagactaga 720 ggaaagacac atgctcaggt ttgtctgaga tggatatacg agcaaggagt tggcctggtg 780 gcgaagagct ttaatgaaga gaggatgaag gagaatttgg agatatttga ttgggcattg 840 actgaagaag agtccaaaca gatcagccag ttaccacaat ataaagggca aaatggagaa 900 acgtttctct cagctgaagg gcctttcaag accttagagg agctatggga tgaagaggta 960 gcagcttga 969 <210> 6 <211> 963 <212> DNA <213> Berberis thunbergii <400> 6 atggaaattg ttcctaaagt gacattgaat tctggctatc aaatgcctct tttaggcttc 60 ggaacagccg aaaattggcc gtttaccaaa tctgaagcag ccaaacgggc aatactgtgt 120 gcaatcaaga atggctacag acactttgac acagcttcca tgtaccaatc agaggagtca 180 cttggtgaag ctatagtcga agcgcttcac cttggcctta tcaaatctcg gaatgaactc 240 ttcatcactt ccaagctatg gtgtagtgat tgtcaccaag atcgcgttct ccctgccctt 300 aagaacacac tccagaatct tcaactggat taccttgatc tttaccttat tcattttccg 360 ctgagttcaa agcgaggtaa acttgagtat ccactacagg aagacgagac ctatcccatg 420 gatttcggat ctgtatggga agagatggaa aagtgccaaa gggttggtct caccaaatcc 480 attggagtca gcaatttctc tgctaagaag attgaacacc tactcacgac cgcaaagatt 540 atccccgccg ttgatcaagt tgagatgaat ccattgtggc aacaaaggac tctgagagag 600 tattgtaaga ccaaaggtat cgttgtgaca gcttactcac ctttaggtgc caaaggtgca 660 atttggggat ccaatatcgt tatggagaat gaggtgctag cagagattgc taaggctaaa 720 ggaaagaccc atgctcaggt atgtctgaga tcagtgtacg aaaaaggcgt tgcattggta 780 acgagaagct acaatgaaga aaggatgaag cagaatctaa acatatttga ttggaacttg 840 agcaaagaag agaccaaacg tatcgacaac ttgccacaaa aaagagtctt ccttggagaa 900 ggatttctgt ctcccctagg acccttcaag tctccagagg aactatggga tggagagatg 960 tga 963 <210> 7 <211> 975 <212> DNA <213> Corydalis chelantifolia <400> 7 atgatggtga gtgttcctga ggtgacactg cagagtagtt ccagtgataa aaagatgcct 60 attatgggca tgggcactgc agcctatcct ttcgccgaat cagatgaagc aaaggctgca 120 ctcttaactt caatcaagct tggttacaga cattttgata cagcttcact gtaccacaat 180 gagaagtctg taggtaaagc tatatctgag gctcttaaac tcggactcgt caaatctcga 240 gatgaattct acatcaactc caagctatgg tgctctgatt ctcacccaga ccgagtcatc 300 cctgcccttc aagagtcact caagaagctt gggttggagt accttgatat gtatcttatt 360 cattggccat tgagcttgaa gaaagggagt tatgagttcc ctataccaac ggacgaggaa 420 atactaccta tggacttcaa ggcagtatgg gcagccatgg aggagtgcca aaagcttggc 480 ctcactaagt ccattggtgt cagcaacttt tcttgtaaga agcttgaaac aattctctct 540 acggccaaga tacctcctgt tgttaatcag gtggagttga acacactttg gcaacagacg 600 aaactaaaca aattttgtaa ggacaatggt atcgttctta tggcattttc ccctttggga 660 gctcacggaa gtccttgggg aacaaaccgt gttatggaat gcgagttact tcatgagatt 720 gcaaaggcaa aaggaaagac tcttgctcag gtttgtctga gatggatata tgagcaaggt 780 gctggtatat tagtaaagag ctacaatgaa gtgcggatga aggaaaacct ggagatattc 840 gattgggaat tgagtgcaga agagttgaaa agtatcagtg aacttccaca gcatagaggc 900 ttcccagctg attctctcct ctcagctaaa gggccattta gaactgaaga agagctctgg 960 gatggagaga tctga 975 <210> 8 <211> 1020 <212> DNA <213> Corydalis chelantifolia <400> 8 atggagagtg ttgcttgtga gcctggagta atcgagcagc agcagtactt tattcccgag 60 gtgacctgcg gtggaggcag cagcaccacc aggatgagga tgcctgttgt aggcatgggt 120 acagcctcgt acccgtttgc tggatcagaa ggtgtaaagc aagctctctt acatgcaatc 180 aagcttggtt actggcattt tgatacagct tctttttacc agaccgagca atctctgggt 240 gaagctatct ctgaggcaat ccaacttggg ctgatcaaat ctagagatga actcttcatc 300 acttctaagc tgtggtgcac tgatgcacac aaagatcttg tcctccctgc ccttcaaaac 360 accctcagga atcttcaatt ggagtacctt gacctttacc ttatacattt tccgataagc 420 ttgaagccag ggagatatga tttccctgta caatcaaagg aggacctcct tccaatggac 480 tttaaatcta tatgggcaaa aatggaagag tgtcagggac ttgggctcac taaatccatt 540 ggtgtcagca atttttcatg caagaaactt gaaaatttac ttgccacagc caagattcct 600 cctgctgtta atcaggtaga gatgaatcca ctttggcaac aaaagaaact aagggagttc 660 tgtaaagcta atggtatcac tattacggcc cactcccctt taggagccaa aggaactaat 720 tggggaacca accgtgtagt agaatgtgaa gtgttgcacc agattgccaa ggctaaagga 780 aaatctcatg ctcaggtttg tttgagatgg gtatatgagc aaggagtgag tctgttggtg 840 aagagcttca acaaagaaag gatgaaagag aacctgggga tatttgactg ggaattgaat 900 gctgaagatt taaagaagat cagtgagatt ccacagggta gaggactacc tgttgatggt 960 tttctttcac caaatggtcc tttcaagtct gaagaagaat tttgggatgg agagatttga 1020 <210> 9 <211> 906 <212> DNA <213> Corydalis chelantifolia <400> 9 atgcctgtag taggctttgg cacggctaaa tttccctttg gtgatgatga agcgattaaa 60 ttggcagttc ttgaaggaat caagcttggt tacagacact tcgacactgc tactaaatat 120 aaatcagaga agcctctcgg tgaaaccatt gttgaagcta ttaaccttgg tttaatcaaa 180 tctcgtgatg aattattcat cacgtccaaa ttatggtgtg gaagcactga gcgcagcctc 240 atcgtacctt cactcaagac gagtttacag aatctccaat tggactacct agatctttat 300 ctcatacatt ggcccctgag gtttagcacc gacgaaactc caacaccagt cccaaaggaa 360 caacttcttc cctttgacac caagtcagtt tggcaagcaa tggaagaatg ccaaaatctc 420 gggctcacga aatcgattgg tgtgagtaat ttctcatgca aaaaacttga agaattacta 480 tccactgcga agatcactcc ggcagttaat caagtggagt tgaacacatc ttggcaacag 540 acgaagatga gagagttctg taaaggaaaa ggtatacata taactggtta ttcacctcta 600 ggttcctatg gaacaaaatg gggagacaac agagttgttg agaatcaagt gttggaggag 660 attggcaagc ctagaggaaa aactgctgtt caggtttgct tgagatggat atatgagcag 720 ggagcaagta tggttgtcaa aagctttaac aaagagagga tgaaggctaa ccttgacatt 780 tttgattgga agttaactga ggaagagttg aagaggatca gtcagcttcc acagcataaa 840 gcatccctac ctactgcctc ctttggcgac catgatgaag ttagggagct tgactatgag 900 atctga 906 <210> 10 <211> 984 <212> DNA <213> Chelidonium majus <400> 10 atggagagtg atagtgcagc agcagcagta gtagttccag taacgactct gagctcaggc 60 attgagatgc cgatgttagg tatgggaact gttgaaaact ttcttccagg ttccgaaaca 120 gtgaagttgg ctcttctgaa tgcaataaaa ttagggtaca ggcacttcga cacagctgct 180 atataccaaa ctgaggagtg tcttggtgaa gctgtagctg aagcacttca acttggtctg 240 atcaaatcta gagaccaact cttcatcact tccaagcttt ggtgttctga cgctcatcct 300 gatcttgtca tccctgctct tcagaactct ctcaggaagc tgaagttgga gtatcttgat 360 ttatatctgg tacattggcc gttaagctca aagccaggga actgtgtttt tccaatactc 420 aaggaggacc tccttccttt ggacttcaag tctgtgtggg cagccatgga agaatgtcaa 480 aagcttggcc tcacaaagtc aattggtgtt agcaacttct cttgtaagaa gcttcaagat 540 ttattgactt cggctatcat ccctcccgtt gttaatcaag tggagatgaa cccacgttgg 600 caacaaaaga aactgagaga gttttgtaag gccagagata ttgtggttac cgcctactcg 660 cctctgggag ccaaaggcac cgtgtatgga tccggtgcag ttatggactg cgaggtgttg 720 caccagattg ccaagattag agggaagtct gttgctcagg tttctctaag atgggtatac 780 cagcaaggtg tgacaccgtt agtgaagagt ttcaatgaag aaaggatgaa ggagaacatg 840 aagatattcg attgggaatt gtacgaagaa gacttaaaaa tgatcgacga gatcccacaa 900 agtagagtca acccttgtta ttttttcctc tcagaaaacg gacccttcaa gactgtagaa 960 gaattctggg atggagaagt ctaa 984 <210> 11 <211> 984 <212> DNA <213> Chelidonium majus <400> 11 atggagagta gtaataatgc agtattagtt ccagtaataa ctctgaactc aggcagggag 60 atgccaattg ttggtatggg aacagctgaa aacctttttc aaggttcaga aagagtgaag 120 ttggctcttt tgactgcaat aaaggtgggt tatagacact ttgatacagc tgctgtttac 180 cagactgagg agtctcttgg tgaagctata gctgaagcac ttcaacttgg actgatcaaa 240 tctagagatg aactcttcat cacttccaag ctgtggttgc ctgattgtca ccatgatctt 300 gtcctccctg ctcttcagaa ttctctcagg aagcttaagt tggagtacct tgacctatat 360 ttgatacatt ggccggtaag ctcgaagcca ggagagatca agcatgttat accaaaggaa 420 gagctcctcc caatggattt caagtctgtg tgggcagcca tggaagaatg tcacaagctt 480 ggcctcgcca agtcaattgg agtcagcaac ttctcttgca agaagcttca agatttatta 540 gtcactgcca acatccctcc tgatgttaac caagtggaga tgaacccact ttggcagcag 600 acgaaactga gggaattttg taaggctcac ggcatcctcg ttgcagctta ctcgccttta 660 ggagccaaag gcactgcatg gggaagaacc aacggagtta tggactctga ggtgctacaa 720 cagattgcca aggctagagg aaagtctatt gcgcaggttt ctctacgatg ggtatatgaa 780 caaggggtgg ttttgttggt gaagagtttc aacgaggaaa ggatgaagga aaacctgaag 840 atattcgatt gggaattaag tggagaagac ttgaaaaaga tcagcgagat cctcccacag 900 cgtagaggac tccctagtca tgtttttgtc tccgatgacg ggccattcaa gtctgaggaa 960 gaactctggg atggagaagt gtga 984 <210> 12 <211> 984 <212> DNA <213> Chelidonium majus <400> 12 atggagagta gtaataatgc agtattagtt ccagtaataa ctctgaactc aggcagggag 60 atgccaattg ttggtatggg aacagctgaa aacctttttc aaggttcaga aagagtgaag 120 ttggctcttt tgactgcaat aaaggtgggt tatagacact ttgatacagc tgctgtttac 180 cagactgagg agtctcttgg tgaagctata gctgaagcac ttcaacttgg actgatcaaa 240 tccagagatg aactcttcgt cacttccaag ttgtggattc ctgattgtca ccatgatctt 300 gtcctccctg ctcttcaaaa ttctctcagg aagcttaagt tggagtacct tgacctctat 360 ttgatacatt ggccagtaag ctctaagcca ggggagctca ggtcacttat accaaaggag 420 gagctccttc caatggattt caagtctgtg tgggtggcca tggaagaatg tcacaagctt 480 ggcctcacaa agtcaattgg tgttagcaac ttctcttgca agaagcttcg agatttattg 540 ctcactgcca acatccctcc tgctgttaac caagtggaga tgaacccgct ttggcaacag 600 aagaaactga gggagttttg taaggccaac ggtatcgtca ttgcagctta ctcgccttta 660 ggagccaaag gcactgcatg gggaagaacc aacgacggag ttatggactc tgagttgcta 720 caaaagattg ccaaggctag aggaaagtct acggcgcagg tttctctacg atggggatat 780 gaacaagggg tgattttgtt ggtgaagagt ttcaatgaag agaggatgaa ggaaaacctg 840 aagacattcg attgggaatt aagtggagaa gacttgaaac agatcaacga gatcctccca 900 cagcgtagag gacatagcca tgaatttatc tcagataacg ggccattcaa gtctgaggaa 960 gagctatggg atggagaagt gtga 984 <210> 13 <211> 987 <212> DNA <213> Chelidonium majus <400> 13 atgggtgagg aaatgatcaa gacgagtagt gttgaaattc cagtagcgac tctaagcact 60 gggaggacga tgccgttgtt aggtatgggg acagcagcat acccatttgt ggggtcagat 120 ggagtggtga aagcgattct gcacgcgatt aagctagggt acaggcactt tgatacagct 180 gctctatact ttacagagga atgtcttggt gatgctgtag ctgaagcact tcgccttgga 240 ttgatcaaat ctcgagatga actcttcatc acctcaaagt tatattgctg tgatgctcat 300 cctgatcgcg tcgtccctgc tcttcagaac tctctcagga agctgaaaat ggagtacctt 360 gatctatatt tgatacattg gccggcaagc acacacgaag ggaacttcga atttccttta 420 caaaagcagg atatccttcc tttggatttc aagtctgtgt gggcagctat ggaagaatgt 480 caaaagcttg gcctcaccaa gtcaattggt gtcagcaact tctcttgcaa gaaacttcaa 540 gatttactag cctcggctaa gatccctccc gctgttaatc aagtggagat gaacccaatt 600 tggcaacaga ataaattgag ggaattctgc aaggcaaatg atatccacat tacagcgtac 660 tcgcctttgg gggccaatgg aactccttgg ggctctagtg gagttgccga aactcaagtg 720 ctgcacgaca ttgctaaggc tagagggaag actcatgctc aggtttgttt gcgatgggta 780 tatgagcaag gtgtgagtgt gttggttaag agttacaatg aagagaggat gaaggagaat 840 ctggctgtat tggattggga attgagcgaa gagaacttga agaagattag tgaaatccca 900 caacgtagag gacttcctgg tgatatcttc gtctcagata acgggccttt caaaactgta 960 gaagagctct gggatggaga agtatga 987 <210> 14 <211> 921 <212> DNA <213> Cocculus trilobus <400> 14 atgccagtgg tggggatggg gttggcagcc tatccatttc aagaatcaga ggtagtgaag 60 ttggcaatgc taagggccat tgagatgggt tacaggcatt tcgacacggc tgcgctgtac 120 cagacagagc agtcacttgg tctagccatt gcagaagcgc ttgagcttgg cttgatcaag 180 tcccgtgatg agctcttcat cacttccaag ctctggtgca gtgatgctca tcctcacctt 240 gtcattcctg ctcttcaaag gactctcaag aatcttgggt tggactatgt tgatctctat 300 ctcatacact ggccagtgag ctcaaacaaa ccaggaaagt atgattttcc agtaccaaag 360 gaagaactag ttcaaatgga ttatgaatct gtatgggcag ccatggaaga gggacatagg 420 cttggcctca caaagtccat tggtgtaagc aacttttctt gtaagaagct tgagaattta 480 ctcacaatag caaaggtccc tcctactgtc aatcaagtgg agatgaaccc cttttggcga 540 caatataaac tgaaagagtt ctgcaaggaa aagggaatta ttatcactgc ttactcacca 600 ttgggagcca aaggaactat ttggggcacc aacaaagtta tggaatctaa agtgcttgaa 660 gacattggaa ttgctagtgg aaagactctt gctcaggtct gtctgagatg ggtgtatgag 720 caaggagtga ctcttttggt aaaaagcttc aatgaggaga ggatgaaggg gaacctagac 780 atatttgatt gggaattaag tgagaaagag ctgaaaaaga tcaatgagat tccacagagc 840 agaggactcc ctggtgacat ctttgtgtca gatgatggac ccttcaagtc tgaagcagag 900 ctctgggatg gagaagtttg a 921 <210> 15 <211> 966 <212> DNA <213> Eschscholzia californica <400> 15 atggggagtt gtagtatacc agtgaagagt ctgagctcag gaagggagat gcctgtaata 60 ggtatgggaa ctgctgacag caatctagca ggctctgatg cttcaaggat agcactagtg 120 aaagctatag aggttggtta tagacacttt gatacagctt caatttacca gagtgagcaa 180 aaccttggag atgccattgc tcaagcactt gaacttggtc tcatcaaatc tagagatgaa 240 ctattcatta cttccaagct atggtcttca gattctcacc ccgatcgtgt cgtccctgct 300 cttcaagaat ccctcaggaa gcttaaattg gagtatcttg atctatatct aatacattgg 360 ccggcaagtt cgacaccagg gatttatgag ttccctatac caaaagatga gatctttcca 420 ttggagtatg agactatttg ggcagccatg gaagaatgtc agaggcttgg gcttacaaaa 480 tctattggtg ttagcaactt ttcttccaag aagctccaaa ctatattgaa caccgccacc 540 atccctccgg ctgccaatca agtggagatg aacccagttt ggcaacagaa gaaattgaaa 600 gagttttgtg acgccaacaa tatcctgctt actgcatatt cgcctttggg tgctaaagga 660 acctcgtggg gaagcaataa agttatggag tctgaggttc tgaaacaaat tgctatggcg 720 aacgggaagt ctgttgctca ggttagtctg agatggttat accaaatagg tgcaactatg 780 gtagtgaaaa gttacaatgc agaaaggatg aaggagaacc tgaagatatt cgattgggag 840 ttgagtgatg aagacatgaa agcaatcagc gagattcctc agcgtcgaaa ttttgctggt 900 gatattctga tttcaagtaa cgggcccttc aaatccgaag acgaactctg ggatggagaa 960 gtatga 966 <210> 16 <211> 972 <212> DNA <213> Eschscholzia californica <400> 16 atgagtagtt atggtagtgt accaatgaag tcactaaact caggaagtaa aattcatgtt 60 ttagggtttg gaacagctgc ttacccattt gtgggatcag atggtgtgaa gaatgccatt 120 cttaatgcta taaagttagg ttataggcat tttgacacag ctgctctcta cttcactgag 180 gagtctcttg gtgaagctgt ttctgaagca cttcaacttg gtctcatcaa atctagagat 240 gaactcttca tcacttccaa gctttggatt tctgatgctt atcctgatcg tgtcctcccc 300 gccattcaaa aaacacttcg gaaccttaaa atggagtacc ttgatctata cctgatacac 360 tggcccatta gtgggaaaga ggggaatttt gatcttcctg tacctaaaga ttgtctccag 420 gaattggact acaagccagt gtgggcagcc atggaagaat gtcaaaagct tggactcact 480 aaatccattg gtgttagcaa tttctcttgt aagaaacttc aaatcattct ctcctcagct 540 accatccctc ctgctgttaa tcaggtggag atgaacccaa tttggcacca gaagaagttg 600 agagaatttt gtaaggccaa taatatcatg attgctgctt attcgccgtt aggagctgct 660 gggaccccat ggggatcctc cggtgtcgtc gagaccgaag tgctacacga gatagccaga 720 agtagaggca aaactcatgc tcaggtttgt ttgagatggg tatatgagca aggggtgatt 780 gtgttggtga agagctttaa tgaagagagg atgaaggaaa acctatctgt gtttgactgg 840 gagttgacgg acgaagactt gaagaaaatc agtcaaattc ctcaacgtag aggacttcct 900 agtggtgata tttgggtctc gacagaagga cctttcagat ctgaagaaga gctttgggat 960 ggagaaatct aa 972 <210> 17 <211> 975 <212> DNA <213> Glaucium Flavum <400> 17 atggagatga gtagtgcaca agtagttcca ctaatgactc taaactcagg caaggagata 60 cctgtattgg gcatgggtac tgctgaaaac catttccaag ggtctgatgg aacaaaagat 120 gcacttgtta atgcaataaa aattggttac agacactttg acacagctgc tatatataac 180 gttgaggagt gtcttggtga tgctatagct gaagcacttc aacatggtct catcaaatcc 240 cgtgacgaac ttttcatcac ttcaaagcta tggatatctg attcccaccc tgaccgtgtc 300 ctctttgctc ttcaaaattc tctccggaag cttaagttgg agtaccttga tctatatctg 360 atacattggc cgttaagctc aacgccaggg aaatcggtgt ttccggtacc caaggaggac 420 ttcctacctt tggacttcaa atctgtgtgg gcagccatgg aagaatgcca aaaacttggc 480 ctcaccaagt caattggtgt cagcaacttc tcttgcaaga agctccaaga tttattggac 540 attgccaaca tccctcctgc agttaatcaa gtggagatga gcccactttg gcaacagaag 600 aaacttaggg agttttgtaa ggtcaatggt atacttgtta cagcttactc gcctttagga 660 gccaaaggca ccttctgggg ctctaatgaa attatggact ctcatgtgtt acaagagatt 720 gctaaggcta gagggaagtc tgttgctcag gttactctca gatgggtata tgagcaagga 780 gtgattttgt tggtgaagag ttttaatgaa aatagaatga aggagaatat ggcgatattt 840 gattgggatt tgagcatcga cgacttgaaa aaattcgatg agatctcaca gcgcagagga 900 aaccttggcg atttttttgt ttcagaaaac gggcccttca agtctgtaga catggtttgg 960 gatggagagg tctaa 975 <210> 18 <211> 981 <212> DNA <213> Glaucium Flavum <400> 18 atggagagta gtagtagtag tgcagtagta gttccagtaa tgactctgaa ctcaggcaag 60 gagatgcctg tattaggtat gggaactgct gaaaacaatc ttcaagggtc tgagaaaaca 120 aaattggctc ttttgactgc tataaaagcc ggttacagac actttgacac agcttctgca 180 tacaatactg aggaggcact aggtgaggct gtggctgaag cacttcaact tggtctcatt 240 aaatctcgag acgaactctt catcacttcc aagctatggt gctctgatgc tcaccctgga 300 cttgttgtcc ctgctcttca aaattcacta cggaatctaa agttggagta tcttgatcta 360 tatctgatac attggccagt aagtttaaag ccgggaaaat tcgtgattcc tttttccaag 420 gaggacatcc ttcctttgga cttcaaaaat gtctgggcag ctatggaaga atgtcaaaag 480 cttggccttg ccaagtcaat tggtgtcagc aacttctctt gcaagaaact ccaagactta 540 ttggcaattg ccaacatccc tcctgctgtt aatcaagtgg agatgagccc actttggcaa 600 cagaagaaac tgagggagtt ttgtaaggtc aatggtatac ttgttacagc ttactcgcct 660 ttaggagcca aaggcacctt ctggggctct aatgaaatta tggactctga tgtgctaaac 720 caaattgcta aggctagagg aaagtctgtt gctcaggttt ctctcagatg ggtacacgag 780 caaggggtga ttttgttagt gaagagtttt aatgaaaata gaatgaagga gaatatggcc 840 atatttgact gggaattgag caacgacgac ttgaaaaaga tggatgagat ctcacagcgt 900 agaggacccc ttgctgatat ttttgtttca gaaaacgggc ccttcaagtc tgtagacatg 960 ctttgggatg gagggttctg a 981 <210> 19 <211> 981 <212> DNA <213> Glaucium Flavum <400> 19 atggggagta gtgatgcagt agtagttcca gtaaagactt tgagctcggg gaggaagatg 60 cctgtaatag gcatgggaac tgctgaaatc cttcttgtgg acgggtccga aaaagtgaag 120 ttggctcttc taaatgctat taaaattggt tacagacact ttgatacagc tgctgtgtac 180 cgaactgaag aggctttggg tgaggttgtg gctgaagcaa ttcaacttgg tctcatcaaa 240 tctagagacg aactattcat cacttcaaag ttatggttat ctgattctca ccctgatctt 300 gttctccctg ctcttcagaa ctctctaagg aagcttaagt tcgagtatct tgacctatat 360 ttgatacatt ggccattaag ctcaaagcca ggggagctga aggttcttat accgaaggag 420 gaactccttc ctttggactt caaatctgtg tgggcagcca tggaagaatg tcaaaaactt 480 ggtctgacaa agtcaattgg agtcagcaac ttctcttgca agaagcttca agatttattg 540 gacattgcca acatcccacc cgcggttaac caagtggaga tgaacccact ttggcaacag 600 aaaaaattga atgagttttg taaagccaat gatatcgtca ttacagctta ctcgcctttg 660 ggagccaaag gcaccccttg gggatccaat agagttatgg actctgaggt attaaaccag 720 attgccaagg ctagaggaaa gtctgttgca caggttgctc ttagatgggt atatgaacaa 780 ggtgtgagtc tagtggtaaa aagttttaac gaagagcgga tgaaggaaaa cgtgacgaag 840 atatttgatt gggaattaag cgcagaagac ttgaaaaaga tcgaggaaat cccacaatgt 900 agaggagtcc ctagccatgt ttttgtctca gttaacgggc ctttcaaatc tgaagaagag 960 ctgtgggatg gtgaaatcta a 981 <210> 20 <211> 984 <212> DNA <213> Glaucium Flavum <400> 20 atgggtgagg aaatgaagat caagagtgtt gaaattccag tagtgacact aagctctgga 60 aggaccatgc ctgtgtttgg tatgggtacc gcagcattcc catttgtagg gtcagatgga 120 gtggtgaaag ccattactca tgctataaag cttgggtata ggcactttga tacggctgca 180 ctctacttca ctgaggagtc tcttggtgat gctatagctg aagcacttcg acttggactg 240 atcaaatctc gagatgaact attcatcact tctaagctat ggtgctgtga tgctcatcct 300 gatcgtgtcc tccctgctct gcgcaactct ttgaggaatc tgaaaataga gtatctagat 360 ctatacttga tacattggcc ggcaagcaca cacgaaggga acttggaatt tccaatacaa 420 aagcaggaca tccatccttt ggattacaat tctgtatggg cagccatgga ggagtgtcaa 480 acgcttggcc tcaccaagtc aattggagtc agcaacttct cttgcaagaa gcttcaacat 540 atattagcca ttgccaagat ccctcctgct gttaatcaag tggagatgaa cccaatttgg 600 caacagaaga aattaagaga attctgcaag gccaatgata tccatattac agcttactcg 660 cctttaggag ctaatgggac cccttggggg tctagtggag ttgttgagac tcaagtacta 720 catgacattg ctaaggctag agggaagact catgctcagg tttgtctaag atgggtatac 780 gagcaagggg tgattgtatt ggtgaagagc tacaatgaag aaaggatgaa agagaatctg 840 actgtattcg attgggaatt gagcgcagag gactcgatga agatcagtga gatcccacag 900 catagaggac ttcctggtga tattttcgtt tcagacagtg ggcctttcaa gtctgaagag 960 gagctttggg atggagaagt ctga 984 <210> 21 <211> 960 <212> DNA <213> Hydrastis canadensis <400> 21 atgggtagtg ttcctaatgt aattcttagc tctggtcatc caatgcctct tgttggcttt 60 ggcactgcgg gttttccatt tggaacatca gaaggtataa agtcagcaat actctgtggg 120 atcaagaatg gttacagaca cttcgatacg gcttccgtgt accaaacaga acagatactt 180 ggtgaagcta tagctgaagc acttgagcta ggcctcatta aatctagaga tgaactcttt 240 ttaacctcca agctatggtg cagtgatgct catcaacaac atgttctccc tgcccttcag 300 aagactctaa ggactcttca gctggattat cttgatcttt atctagtaca ctggccactg 360 agctcaaaac cagggaagta tgagtaccca ataccaaagg aagagcttct tcccatggat 420 ttcaaatctg tctgggctgc aatggaagag tgccaggctc ttggcctcac aaagtccatt 480 ggggtcagca atttctcttg taagaaactt gaacaattac tctccacttc aaacatccct 540 cctgctgtca atcaagtgga ggtcaaccca atttggcaac aaaataagct gagagagttt 600 tgtaaggcca aaggtataat tgttgctgct ttttctcctt tgggtgctaa aggaacatct 660 tggggaacta ataaagttat ggattctgag gtgctgaatg agattgccca ggctagagga 720 aagactactg ctcagaattg tcttagatgg ttacatgagc aaggtgtgtg tgtggtggtg 780 aagagcttca atgaggagag gatgaagggg aacctgaaat tatttgattg ggaattgagt 840 aaggaagagt ctaagaagat cagccagtta ccgcagagta aaggacatac cggagatgac 900 atggtctcgg ccaatgggcc atttaagtct ttagaggagc tatgggatgg agagatttga 960 <210> 22 <211> 960 <212> DNA <213> Hydrastis canadensis <400> 22 atgacaagaa ttccagagat tgtgttgaac tcaggatgga ggatgccagt tttggggatg 60 ggaacagcaa cctttcctat ccaatcccca gaagtcattg agtcttccat tgttaacgcc 120 atcgaattgg gttatcgaca cttcgacaca gctagtgtat atcagtctga gtcacctcta 180 ggccgtgcca tatcagaagc tatacgtcga ggcctcattg agagtcgaaa agaggtcttc 240 atcacctcga agctatggtg cactgatgca caccatgacc ttgttatccc agctctccac 300 aaaactctcc agaatctggg gttggagtac ttggacttgt acctgattca tttcccagtg 360 agactgaaag gagacatatc ctttgacatt aagaaggcag accttatacc ctttgatgta 420 aagggtacat gggaggccat ggagaagtgt caagagctgg gacttaccag atctattggt 480 gttagcaact tttcatctaa gaagctttcc gagcttttaa cccatgctac catttctccc 540 gctgtgaatc aggtggaaat gcacccattt tggcaacaga aggaattgag agctttctgt 600 gcagacaaag gtatacatgt aagtgcttat tctcctttgg gaggaaaggg tgctttatgg 660 ggctccgaca tattactcaa ctccaaagag atcgaacgga tcgctcaagc taaaggaaag 720 agcatcgcgc aggtatgctt gagatgggca tatgaacaag gggtgagtta cttaccaaaa 780 agctacaata agggaaggat gaaagagaac atggaaattt ttgactggca attgagtgaa 840 gatgagttac aaaagatcag tcacctgcca caaggaaaga tatacacagg acatcatttt 900 atatcagatg atggggaata taaatctcct attgatttat gggattgtga gatatgttag 960 <210> 23 <211> 963 <212> DNA <213> Hydrastis canadensis <400> 23 atgggtatag ttcgtgaggt agtgttgaat tctggggaaa gaatgccatt gctaggaatg 60 ggaacagcaa catacccagt tgctccattt gaactagttg agtcttcagt aattgctgcc 120 attgaacttg gttataggca ttttgacaca gcaagtgtgt atgagacaga gcaacctcta 180 ggcctagcca tatctgaagc cgtccggcaa ggccttatcg ctagtcgtga tgaaatcttc 240 ataaccacta agctttggtg tggtcatgca cattatgatc ttgttctacc agctcttcgg 300 gactcactag aaacgatggg gttggattat gttgatctgt acttgattca ttttcctgca 360 agattcaaca tgaaagagaa gagcttaaat gtgaataaga aagatcttct tcctttggat 420 ataagaggga catggaaagc tatggaggaa tgttatgaac ttggcttagc aaagtcaatt 480 ggtgtcagca acttcagttg caaaaagcta tctcaacttt tgtctgttgc taacattccg 540 cctgcagtga atcaggtgga gatgcatcct ctttggcaac aacagaagtt gagagagttt 600 tgtgaagaaa aaggtataca tgtgagtgct tattctcctt taggaggaaa agggaccata 660 tggggctcca acgcagtttt ggactcagat caaatcaaac aaatagctaa ggctaaaggg 720 aagagtattg ctcagatttg tttgagatgg ggatttgaac atggagtgag cattttacca 780 aagagcttca acaaagaaag gttgaaagaa aatatggaga tatttgattg ggagctaagc 840 aaggaagagt tacaaaagat gaataccttt cctcaaaata gaatttttca agctgagcat 900 ttagtctcac ctgatgaggg actgtttaaa tctgttgctg atttgtggga tggtgaaatc 960 taa 963 <210> 24 <211> 954 <212> DNA <213> Jeffersonia diphylla <400> 24 atggggattg ttcctgaggt gacattgaac tctggccatc agatgcctct tgtaggcttt 60 ggaacagcac agtttccttt tccagagggg gatgaagcaa aacaaattat agctccaata 120 ctatgtggaa tcaagagtgg ttacagacac ttcgacacag cttccctgta caaaactgag 180 gaagcacttg gagaagctat aaaagaggca cttcgtcttg gccttatcaa atctcgagac 240 gagctattca ttacctcgaa gctttggtgt actgattctc atcaagatct tgttcttcct 300 gcccttaaga agactctcca gaatcttcag atggactacc ttgatctata ccttgtacat 360 tttccagtaa gctcaaagcc gggaaagcca gagttcccac cacagaaaga ggaccttctt 420 cctatggatt ttggatcggt atgggcagca atggaagagt gccagaggct cggtctaacc 480 aaatcaattg gagtcagtaa tttctcttgc aaaaagttag agcaactact cacaacagca 540 aagattattc ccgcggttaa cgaagtggag atgagcccag tttgccaaca gaataagctg 600 agagagtttt gtaaggtaaa aaacattgtt gtgactgctt actctcctct aggtggagga 660 agcaatgcag ttaaggacaa taaggtgctg aaagaaattg ccaaggctaa aggaaaaaca 720 tgtgctcagg tcactctgag atgggtatac gagcaaggtg ttgctctggt gcctaagagc 780 ttcaatgagg ggaggatgaa ggaaaacctg gatatattta attggacaat aagtgaagaa 840 gagttcaaac agatcagcca gttaccacag ggtagagtgg caactggaga atggtttgtg 900 tcggttgaag ggccattcaa gtctctagag gaactatggg atgaagtgat ctga 954 <210> 25 <211> 966 <212> DNA <213> Mahonia aquifolium <400> 25 atgggtgttg tacctgtggt aaccctgaac tctggtcatg agatgccact agtaggcttt 60 ggaacagcag tgcctgcctt cgggggatca gacgccataa agcaagcaat actatgtgga 120 atcaagaatg gttacagaca cttcgacact gcttctgtat accaaacaga gaagtcgctt 180 ggagaagcta tagtggaagc gcttcgcctt ggcctcatca aatctcgaga tgaactcttc 240 attacctcca agttatggtg tacagatgct catcaagatg gcgttctccc tgcccttagg 300 gagactctca agaatcttca gttggattac cttgatcttt accttataca ttggccgcta 360 agcgcaaagc cagggaagca tgagtaccaa ataccaaagg atgagcttct tcctatggac 420 tatgaatccg tatggggagc aatggaagaa tgccagaggc ttgacctctc taagtccatt 480 ggagtcagca acttctctat caagaagctt gaagagctac tcaaaactgc aaagattatt 540 cctgcagtta atgaagtgga agtgaaccct gtttggcatc aaaataagct aatagagttc 600 tgtaaggcca aaggtattgt tgtgactggt tactctcctt taggtgccaa aggaacaact 660 tggggaacca atagagtcat ggacaatgag gtcctgaaag agattgctaa gactagagga 720 aagacccatg ctcaggtttg tctgagatgg atatacgagc aaggagttgg cctggtggcg 780 aagagcttta atgaagagag gatgaaggag aatttagaga tatttgattg ggcattgact 840 gaagaagagt ctaaacagat cagccagtta ccacaatata aagggcaaaa tggagaaacg 900 tttctctcag ctgaagggcc tttcaagacc ttagaggagc tatgggatga agagatagca 960 gcttga 966 <210> 26 <211> 963 <212> DNA <213> Menispermum canadense <400> 26 atggcgagtg ttccagaggt gacactgagc tctggccatc caatgcctct ctttggcttc 60 ggcaccgcag cctggccttt tgtgtcttca gaaggagcta agtctgcaat attgtgtggg 120 atggagctcg gttacagaca cttcgacact gcttccattt acggcacaga gagctctctc 180 ggcgaagcca tagccgaagc tcttaagctc ggcatcatca agtctcgcga cgagctcttc 240 atcacctcta agctctggtg ctgcgacggg caccatgacc gagtcttgcc tgcccttcaa 300 atgactctaa agaatcttca attggactac attgatctgt atctcgaaca ctggccattg 360 agctcagtcc ctaaaggtga gcttgagtac ccaataccaa aagaaaaact gtttcctatg 420 gatttgaagg ctgtgtggga agccatggaa gagtgccaga ggcttggcct caccaagtcc 480 attggagtca gcaacttctc ttgcaagaag cttgaagatt tactcgcctt cgcgaagatc 540 cctccggccg tcaatgaagt ggagatgaac ccagtttggc aacagaagaa actgagggac 600 ttctgtaagg ctaaaggtat tgttttgacg gcttactcag tgttaggagc caaaggaacc 660 ctttggggca ctaacaaagt tatggactct gaggtgctag gaaagattgc caaggcaaga 720 ggcaagactg ttggtcaggt ttgtttgaga tgggcatatg agcaaggtgc gtgtgtgttg 780 gtgaagagct tcaatgagga aagaatgaaa gagaacttag agacatttaa ctgggagtta 840 ggagaggaag acttgaagat gattagtgaa ataccacaat ataaaggact tcgtggtgag 900 gatatggtgg ctcctaatgg gccttacaag actttagagg aactttggga tggagagata 960 tga 963 <210> 27 <211> 1026 <212> DNA <213> Menispermum canadense <400> 27 atgggagttg ataagacaaa cgagttctct aatggcaacg cagcgagccc agtactgatc 60 aaagaggcta acctctttaa gattgccgag gtgacgttga actccggtca ccatatgccg 120 gttctgggaa ctgggacggc ttcgtttccg tttcctccgt tgaaggagct gaagaaggcg 180 atcgtggaag ccatggaagt gggctatcgc cacttcgact cggccgcgct gtaccagagc 240 gaggaagggc tcggtgcagc catagctgag gctttggaga agggacttgt gaagaagaga 300 gaagaactat tcatcaccac caagctatgg tgcaataatg cccactctga tcgtgtcctg 360 cccgccattc gtgaatctct caggaaacta agactagact atgtagattt gtatttgatt 420 catttcccag taaggctgaa ggaagattta ttggatatga attgtaagaa gggtgggatt 480 tttgaacttg acctcaaatc agtatgggca gcaatggagc agatacaaca cctaggtctt 540 gcaaagtcaa ttggagtcag caacttcact tgtaagaagc ttactgacct tctttcttat 600 gcaaagatcc ctcctgcagt taatcaggta gaaatgcatc cagtgtggca acaaaagaag 660 cttagagact tctgcaagga gaaaggcatc catgttagtg catattctcc tctaggagca 720 aagcaatggg gatttgatgt tgttctcggt aacaaaatct tgaaagagat tgcacaagac 780 aaaggaaaga caattgctca gattgcttta agatggggat atgagcaagg agtgattttg 840 atcccaaaga gctttaacaa gggaaggttg actgaaaatc ttcgcatatt tgactggaag 900 ctaactgaag atgaattaaa gaagatttca tcaattcaac agagccgagt agctataatg 960 cctgaatttg tgtttcctga aagtccattc aagacctttg aagatttctg ggatggggaa 1020 atgtga 1026 <210> 28 <211> 969 <212> DNA <213> Menispermum canadense <400> 28 atggaaggag gaggagttcc taagatactc ttgaattcag ggcataggat gcctgtgctt 60 ggaatgggca cagcttcctt cccaatctca ccacaacacc tcgtcaagtc ttccatacta 120 accgctatcg aacttggcta cacccacttc gacaccgcaa gcgtctatga gactgagcct 180 acactcagcc gggctatccg ccaagccctc gagggtagac acattgcgag ccgagaccag 240 ctcttcatca ccaccaagct ctggtgcggc caagcacaaa gcgatctcgt cgtcccagct 300 cttcgcgaat cacttcagga acttgggttg gactatgttg atctgtactt gattcatttt 360 ccagctagat ttaagtgtgt ggagaagaca tttaatgtca ccaaagagga tcttcttcct 420 ttggacatga aggggacatg gcaggccatg gaggagtgtt gtaaacttgg cctggccaag 480 tccattggtg tcagcaactt cagctgcaag aagctctctc agattctcac ctttgccacc 540 atccctcctg cagtcaatca ggtggaaatg cacccattat ggcagcaaag gaaactgaga 600 gagttctgta aggagaaagg catccatgtg agtgcctact ctcctttagg tgggaaaggt 660 gccaagtggg gttccaatgc agtgtttgaa tgtgaagaaa tcaaacagat tgctgaagct 720 aaagggaaga gtttggctca gatttgtctg aggtgggctt ttgagcaagg tgtgagtttt 780 gtgccaaaga gctttaacaa ggaaaggttg aaagagaaca tggagatata tggttgggag 840 ctgagcaagg aggaattgca aaagttaagt gttctgccac agtgcaggat cttcaaagga 900 gaatggctag tgtcagctga tcatggagtg ttcaaatcag ttgaagattt gtgggatgga 960 gaaatataa 969 <210> 29 <211> 960 <212> DNA <213> Nandina domestica <400> 29 atggtgagtg ttccagaggt aaccctgaac tccggtcacc ggatgcctct tgtgggtatg 60 ggcacggcat gctttcctct tccaggatca gaagtggtga agtcggcgat ggtaacggca 120 atcaagcttg gttatcgcca cttcgatacc gctgcgcttt accagagcga gcagcctcta 180 ggtgaagcta tagctgaagc acttcacctt ggcctcataa tatctaggga tgaacttttt 240 atcacctcca agctatggtg tactgatgct caccaagatc ttgttctccc tgctcttaag 300 acaagtctcc gaaatcttca gttggattac cttgatcttt accttataca ttttccggtg 360 agtttgaagc cagggaagat acagctgccg gtaccaaagg aagagcttct tccaatggat 420 ttcaagtctg tgtgggcagc catggaggaa tgtcagaagc ttggcctcac aaagtccatt 480 ggagtaagca atttctcttg caagaaactc gaacacatac tttcattggc aaagattcct 540 cctgcagtta atcaagtgga gctgaaccca atttggcgac aaaaaaagct catcgagttt 600 tgtaaggcca aaggtattgt tgttacggca tattctcctt tgggagctgt agcacccggt 660 caaggcagca atagagtcat ggaatgtgaa gtgctgagcg agattgcaaa agctcgagga 720 aaaactcatg cgcaggtttg tcttagatgg ataattgagc aaggggtcag tttagttgtg 780 aaaagcttca atgaggacag gaagaagaag aacttggaga tattcgactg ggaattgagc 840 gaagaagatt ccaaaaaaat cagccagata ccagagacta gaggaaaccc cggagatttt 900 gtcatctctg ttgatggtcc ttataagtct agagaggagc tttgggatgg agaaatctga 960 <210> 30 <211> 960 <212> DNA <213> Nandina domestica <400> 30 atgggaagag ttcctgagac taccttgagt tctgggcata acatgccatt gttggggatg 60 ggaacagcaa ccttccctct ccctccatct gaattgatag aatcttatat tcttgctgcc 120 attgaaatgg ggtatagaca ctttgacact gctagtgttt acgacacaga agtacccctt 180 ggtcgagcca tatctgaagc cctccggaga ggtcttgtgg caaatcgtga tgaactcttc 240 attaccacca aactctggtg tggtcatgca catccagatc tcgttgttcc agctcttcac 300 caatctctca tggaaatggg attggaatat gtggatttgt atttgattca ttttccagct 360 aggtttaaca aaaaggaaaa gaactttgat gtgaagaaag aggaagtcat tcccttggac 420 atgaagggaa catgggaagc catggaagag tgtagcaaac ttggacttgc aaagtccatt 480 ggtgtaagca acttcagctg caaaaagctc tctcaactcc tcacctatgc cactatccct 540 cctgcagtga atcaggtaga aatgcaccct ctttggcaac aatggaaatt gagagaattt 600 tgcgtagcaa atgatataca tgtgactgcc tattctcctc taggggggaa aggagcattg 660 tggggttcga attcagtgct cgattcaaaa gaaatccaac aaatggctga agcaaaagga 720 aagagtatcg ctcagatatg tttgagatgg gcatttgaac aaggagtaag cttcataccg 780 aaaagcttca atgtcgaaag gctaaaagaa aatatggaaa tatttgattg ggaaatgaac 840 aagaatgagt tgttgaaaat gagtttgctt ccacaaaaaa ggactttcac tgcagaatat 900 ttggtatcac ctgatggggc gtttaaatcg gttgacgatt tatgggatgg ggagatataa 960 <210> 31 <211> 960 <212> DNA <213> Nigella sativa <400> 31 atggcaagag tacctgagat aaccttaaac tccggccacc ggatgcctct cttgggtatg 60 ggaactgcag tagttccctt taaagaacca gaaccagtga agtcagcaat actcactgca 120 atcaagaatg gttacaggca cttcgataca gcttctctgt accgaactga gccagccctt 180 ggagatgcta tagctgaagc acttcaactt ggcctcatca actccaggga cgaccttttc 240 atcacctcca agctctggtg cactgacaac caccctgatc tggtcctccc tgcactgcac 300 acaacacttc gaaatctgaa gttggactac cttgatctat atctaataca ttggccagta 360 agcatgactc cagggagaat tcggttccct gggccggatg agaagatact tccaatggat 420 tacaagtctg tatgggcagc catggaagag tgtcaaaagc ttggcctcac aaaatcaatt 480 ggagtcagca attttacctg caagaagctt gaattgttac tggcatcagc tactatccct 540 cctgctgtta atcaagtgga ggtgaaccct atgtggcaac aggaaaagct gatagagttt 600 tgtaggacta aaggtattgt tgtaacagct tacgctccct tggggactgt tggaacattt 660 ataggtaaca acgatattat gaactgtgag ttactgaaag aaattgccca agctaaagga 720 aagacaaatg gtcagatttg tctcagatgg gtacatgaac aaggagtggg tctgctagta 780 aagagcttca ctgaggtgag aatgaaacag aatcttgaga tatttgattg ggaactaagt 840 gaagaagaat caatgaaaat taaacaactg ccacaaagaa agagtcatca agggaagggc 900 tttgttactg ctgatggacc attcaaatcg ttgacagagc tttgggatgg agagatgtga 960 <210> 32 <211> 954 <212> DNA <213> Nigella sativa <400> 32 atggtttatg agatcatgtt gaactctgga tacagaattc cattgctggg aatgggaaca 60 gcagcctatc cagtgcctcc accagagcta gtggagtctt ctgttcttgc tgcaattgaa 120 ctcggttata ggcactttga cactgcccat atttatggaa cggaagcacc tttgggaaga 180 gccatatcag aagccttgag gaagggattg atcaaaagtc gagatgaact cttcataact 240 actaagctct ggcctggcca tgcacgccct gatcttgttc tacaagcttt gcatcaatct 300 ttagaagctt tgaggattga ttacgttgat ctgttcctaa ttcattttcc agcacggtac 360 aacacaacag aaataagaat ggatataaag aaggaagaca tccttccttt ggatatcaag 420 ggaacatggc aggccatgga agagtgttgt aaacttggct tggccaagtc catcggtgtc 480 agcaacttcg gttgcaagaa gctatcccaa ctattggatt cagccactat cccacctgca 540 gttaatcagg tggaaatgca tcctctctgg caacaagcaa aactgagaga gttttgtgca 600 gaaaggggta tccatgtcag tgcctattct cctttaggag gaaaggggac tctctgggga 660 tccaatgcag tgcttgattc tgagcaaatc aaacagattg cacttgctaa ggggaagagt 720 gtagcgcaga tatgcttgag atggggtttt caacaaggag taagcatcat agcaaagagc 780 ttcaacagag aaagactcaa agaaaatatg gaaatatttg attgggaatt gaacaaggaa 840 gaactataca agctaagcat ccttcctcaa aataggattt caacccttga atatttagtg 900 tcaccggatg cagttttcaa atcaattaac gatttgtggg atgaagaagt ttga 954 <210> 33 <211> 957 <212> DNA <213> Nigella sativa <400> 33 atggataaca gtgtagtagt gctgaattct ggacatagaa tgccattgtt agggatggga 60 acagcagcca ctcctttacc tcccaatgaa gttgtccagt cttcagttct tgctgcaatc 120 gaactgggtt acaggcattt tgatacagca agtgtgtatg gctcagaagt gattttaggt 180 agagccatat ctgaagcttt aaggagaggt ttggttgcta gtagagatga gcttttcata 240 actactaagc tttggtgtgg taatggacac cctgatcttg ttcttggtgc tcttcaagaa 300 tctttaagaa ttatggagct ggattatgtg gacttgtact tgattcattt tcctgtgaga 360 ttcaatataa cagagaagct cttaaatatg aagaatgttc ctcttcttcc ttttgacatg 420 gaagggacat ggcaagctat ggaggagtgt tgtagactgg gtttagccaa gtccattggt 480 gttagcaatt ttagttgcaa aaggctatct gaacttctac tttccgcttc catccctcct 540 gctgtgaatc aggtggaaat gcatccgctt tggcagcaaa gaaatatgag agagttttgt 600 agaaaagaag gaattcatgt gagtgcttat tctcctttag gaggaaaagg agcagcttgg 660 gggtcgaatg cagtgctaga ttctgaagat atccaacaaa tagctcaaca taaagaaaag 720 agtgttgctc aggtatgctt gagatgggcg tttgagcaag gagtgagcat tatagtaaag 780 agtttcaata aagaaaggct taaagaaaat acacaaattt tcgactggga gctcaacaaa 840 caagagttgc agagaatcaa tactcttcca caaaataaga tttttaaagc ttcaaatttg 900 atttcagcag atggaccata taaaacattt gaggaattgt gggatggaga ggtatga 957 <210> 34 <211> 960 <212> DNA <213> Papaver Bracteatum <400> 34 atggagagta atggtgtacc tatgatcact ctcagttccg gcattcggat gcctgcttta 60 ggtatgggaa cagttgaaac aatggaaaag ggaacagaaa gagagaaatt ggcgtttttg 120 aaagcgatag aggtcggtta cagacacttc gatgcagctg ctgcatatca aactgaagag 180 tgtcttggtg aagctatagc tgaagcactt caacttggtc taattaaatc tcgagatgaa 240 ctcttcatca cttccaagct ctggtgcact gatgctcacg ctgatctcgt cctccctgct 300 cttcagaatt ctctgaggaa tcttaaattg gagtatctgg atctatattt gatacacttt 360 ccgctaagct tgaaaccagg gaagattgtt aacgaaatac caaaggatca aatgcttcca 420 atggactaca aatctgtgtg ggcagccatg gaagagtgtc agacccttgg cttcactagg 480 gcaatcggtg tcagcaattt ctcatgcaaa aagcttcaag agttgatggc gacagccaac 540 agccctccag ttgtaaatga agtggagatg agcccgattt tccaacaaaa aaaattgaga 600 gcatattgca aggccaataa tatcatgatc actgcatact cggttttggg atccagagga 660 gccgcatggg gcagcaatgc agttatggat tctaaggtgc ttcacgagat tgctgtgtcc 720 agaggaaaat ctgttgctca ggttagtatg agatgggttt accagcaagg cgcgtgtctt 780 gtggtgaaaa gtttcaatga agagagaatg aaggaaaacc ttaagatatt cgattgggag 840 ctatcggcag aagacatgga aaagatcagt gagattccgc aatgtagaac aagctctgtt 900 gatttcttgt tatcaccgac tgggcctttc aaaactgaag aagagttctg ggatgagtag 960 <210> 35 <211> 966 <212> DNA <213> Papaver Bracteatum <400> 35 atggagatta atggtgtacc agtgatcagt ctaagctcgg gcgttcggat gcctgcttta 60 ggtatgggaa cagctgaaac aatggaaaaa ggaaccgaca gagagagatc agcgtttttg 120 aaagcgattg aggtcggtta caggcacttc gatacagctg ctgcttatca aactgaagag 180 tgtcttggtg aagctatagc tgaagcactt caacttggtc taattaaatc tcgagatgaa 240 ctcttcatca cttccaagct ctggtgcact gatgctcacg ctgatctcgt cctccctgct 300 cttcagaatt ctctgaggaa tctcaaattg gagtatcttg atctatattt gatacacgct 360 ccggtaagct tgaagccagg gaagattctt aacgaaatac caaaggatca aatgcttcca 420 atggactaca aatctgtgtg ggcagccatg gaagagtgtc agacccttgg cttcactagg 480 gcaatcggtg tcagcaattt ctcatgcaaa aagcttcaag agttgatggc gacagctaat 540 agccctccag tggtaaatga agtggagatg agccccactt tacatcaaaa aaatctgaga 600 gaatattgca aggccaataa tatcatgatc attgcatact cggttttggg agccagagga 660 accggatggg gcagcaacgc agttatggat tctaaggtgc ttcaccagat tgctgtggcc 720 agaggaaaat ctgttgctca ggttagcatg agatgggttt accagcaagg tgcgtgtctt 780 gtggtaaaaa gtttcaatga agagaggatg aaggaaaacc ttaaaatatt cgattgggaa 840 ctaacggaag aagacatgga taagatcagt gagattccgc aatccagaac actatctgct 900 gatttcttgt tatcaccgac tggacctttc aaaactgaag aagagttctg ggatgaaatg 960 gtttga 966 <210> 36 <211> 972 <212> DNA <213> Papaver Bracteatum <400> 36 atgaggaata ctggtgtacc tgtaatcact ctgagctcgg ggaaggggat acctgtttta 60 ggtatgggaa catttgaaac agttggtaaa gggggtgaac gagagaggtt ggcgtttttg 120 aaagcgatag aggtgggtta cagacacttc gatactgctg cgtgctacca aactgaagag 180 tgtcttggtg aagctataga ggaagcactt caacttggcc taatcaaatc tcgagatgaa 240 ctctttatca cttctaagct ctggtgcacc gacgctcacc ctgaccgtgt cctgttggct 300 cttcagaatt ctctgaggaa tcttaagttg gagtatctgg atctatacct gatacacttt 360 ccagtaagct tgaagccagg gaatgaggtt actatggatg cagcaggggg cgaaattttt 420 ctaatggact acaagtctgt atgggcagcc atggaagagt gtcagaacct tggcttcact 480 aagtcaatcg gtgtcagcaa tttctcctgc aaaaagcttc aggaattatt ggcgacagca 540 aacatccctc cagttgtaaa tcaagtggag atgagcccag ttttccacca aaagaatctg 600 agagagtatt gcaaggccaa taatatactg gtggctgcat actcgatttt gggaggcaag 660 ggaaccgcat ggggctccaa ttcagttttg ggttccgagg ggcttaacca gattgctatc 720 gccagaggaa agtctattgc tcaggttagc atgagatggg tatacgagca aggcgcgatt 780 cttgtggtga aaagtttcag tgaaaagaat atgagggaaa acttgaacat attcgattgg 840 gaactgacta aggaagacct ggaaaggatt ggtgagattc ctcagcgcag attaataatt 900 caggaattta tggtatcatc taatggacct ttcaaatctc tagaagagtt ctgggatgaa 960 aaagctgatt ga 972 <210> 37 <211> 966 <212> DNA <213> Papaver Bracteatum <400> 37 atggagaatg taatacctgc agtgacatta agttctggaa gtgtgatgcc tatattaggt 60 atgggcactg ctgcataccc attagttgaa ccagaagaag cgaaattggc gtttttgaat 120 gctatcaaga ttggttacag acattttgat acagctgctt cttaccattg tgaaggattt 180 cttggtgaag ctatagctga agcacttcaa cttggtttga taaaatctcg agacgaactg 240 tttatcactt ctaagctctg gccctgtgat gctcaccctg accttgtcat ccccgcaatt 300 cagaactctc taaggaatct aaagttggag taccttgatc tatatctgat acattttcca 360 ataagcacga aaccagctgc agggcttgtt tttccgccac caaaggatgc tttgcttcca 420 atggattaca agtctgtatg ggcagctatg gaagagtgtc aaaaacttgg tcttacaaag 480 tcaattggtg tcagcaactt ctcttgtaag aagcttcaaa ccattttgga cattgctaac 540 atccctccag ctgttaacca agtggagatg aacccagttt ggcagaactt gaaattgagg 600 gacttctgca aggctaacaa tatcgttctc actgcctact cacctttagg agccagggga 660 accccgtggg ggtctaatgc agtctatgag gagcgagttc tgcatgaaat tgctgaggct 720 aaagggaaaa ctcatgcaca ggtgtgtcta agatgggtat acgagcaagg agtgagtctt 780 atagtcaaga gcttcaatga gttgaggatg aaggagaata tgatgatttt cgactgggaa 840 ctgactgaag atgaactgca aaagataggc aaaatcccac agaggagagg actcccaggt 900 gatttttttg tctcggaagc agcgcctttc aagactgtcg aagaattttg ggacggagaa 960 atttga 966 <210> 38 <211> 969 <212> DNA <213> Papaver Bracteatum <400> 38 atggaggttg taatccctaa agtggcactg agttctggta gagtgatgcc tgtgctagga 60 atgggcacat catcattccc gccagttggt ccggaagatg gaaaagccgc cattttgaat 120 gctatcaaga ttggttatag acactttgat acagcttctg tttacaagtc tgaagacttc 180 ctcggtgaag ctatagctga agcacttcta cttggattga tccaatctcg agaccagctt 240 ttcattactt ccaagttata ttgcaatgat gctcaccctg atcttgttgt ccctgcttta 300 cagaactctc tcaggaatct gaggctggag taccttgatc tatatctgat acactttccg 360 gtaagctcaa agccagtcaa gtacgagtat catctaaaaa aggagcatct cctccctatg 420 gactacgaat ctgtgtgggc agccatggaa aagtgtcaaa agcttggact tacaaagtca 480 attggagtca gcaatttctc ttgcaagaag cttcaaacta ttttggacac tgctaacatc 540 tctcctgctg ttaatcaagt ggagatgaat ccagtttggc aaaacttgaa actgagggat 600 ttctgcaaag ctaaaggtat cgttgtcact gcctactcac ctttgggagc cagcggaacc 660 ccctggggat ctaatgcagt caaagaagca caggaattgc acgaaatcgc aaaagctaga 720 gggaaaactc atgctcaggt ttgtcttaga tgggtatacg agcaaggagt gagcttgcta 780 gtcaagagct tcaaagaaga acggatgaaa gagaacctaa tgatctttga ttgggagttg 840 actaaagatg atttgttcaa aatcagcaaa attacacaac gcagaggact accaggttat 900 aggttcatct cgaaacttga agggtcaccc ttcaagacgg tcgaagaatt ctgggatgga 960 gaagtttaa 969 <210> 39 <211> 966 <212> DNA <213> Papaver Bracteatum <400> 39 atggagagta gtggtgtacc agtaatcact ctgagatcgg gcaaggtgat gcctgtttta 60 ggcatgggaa catttgagaa agctggtaaa gggtctgaaa gagagaggtt ggcgatatta 120 aaagcgatag aggtgggtta cagatacttc gacacagctg ctgcatacga aactgaagag 180 gttcttggag aagctattgc tgaagcactt caacttggcc taatcaaatc tcgagatgaa 240 cttttcatca gttccatgct ctggtgcact gatgctcacc ctgatcgtgt cctcctcgct 300 cttcagaatt ctctgaggaa tcttaaattg gagtatgtgg atctatatat gttacccttc 360 ccggcaagct tgaagccagg gaagataacg atggacatac cagaggaaga tatttgtcca 420 atggactaca ggtctgtatg gtcagccatg gaagagtgtc aaaaccttgg cctcactaaa 480 tcaatcggtg ttagcaattt ctcctgcaaa aaacttgagg aattgatggc gaccgccaac 540 atccctccag ccgtgaatca agtggagatg agcccggctt tccaacaaaa gaagctgaga 600 gagtattgca acgcaaataa tatattagtc agcgcagtct ctatactggg atcaaacgga 660 accccatggg gctccaatgc agttttgggt tctgaggtgc ttaagaaaat tgctatggcc 720 aaaggaaaat ctgttgctca ggttagtatg agatgggttt acgagcaagg cgcgagtctt 780 gtggtaaaaa gtttcagtga agagagattg agggaaaact tgaacatatt cgactggcaa 840 ctcaccaagg aagacaatga aaagatcggt gagattccac agtgcagaat cttgagtgct 900 tattttttgg tctcacctaa agggcctttc aaatctcaag aagagttgtg ggatgacaaa 960 gcttga 966 <210> 40 <211> 972 <212> DNA <213> Papaver Bracteatum <400> 40 atgggaagta gttgtattcc tgttctcact ttgaactcgg gcaataagat gcctgtttta 60 ggtatgggaa catttgaaac atttgctaaa gggggtgaaa gagaaagatt ggcgtatttg 120 aaagcgatta aagtgggtta cagatacttc gatacaggcg ctgcatacgg aacggaagag 180 gttcttggcc aagctatagc tgaagcactt caacttggcc taataaaatc tcgagatgaa 240 ctcttcatca gtaccatgat ctgggccact gatgctcacc ctgatggtgt cctccccgct 300 gttcagagat ctttaaggaa tcttaaattg gactatgtgg atctctacct gataccattt 360 ccggcaagct tgaacccaga aggggagata cctaattaca taccagagaa cgaaactttt 420 ttaaaaatgg actacaagtc tgtatgggca gctatggaag agtgtcaaac ccttggcttt 480 accaagtcta tcggtgtcag caatttctcc tgcaaaaagc ttcaggaagt gatggagacc 540 gcaaatatcc ctccggctgt caacatggtg gaaatgaacc ctactttcca acaaaaatat 600 ctgagagagt actgcaaggc caataatata ttagtcaatg cgtactctgt tttgggatcc 660 acgggaactt catggggctc caatgcagtt atgggttctg aggtgcttaa gcaaattgct 720 acggacatag gaaaatctat tgctcaggtt agtatgagat gggtttacga gcaaggtgcg 780 ggttttgtgg taaaaagttt cagtgaagag aggatgaggg aaaacttgaa catattcgac 840 tgggagctga ctaaggaaga cttggagaag atcagtcaga ttccacaatg cagagtacta 900 cctatggatt ttttggtatc atctgatgga gctttcaaat ctttggaaga cttgtgggat 960 ggtgaagctt ga 972 <210> 41 <211> 912 <212> DNA <213> Papaver Bracteatum <400> 41 atgccgatct taggttttgg aacagctgaa aacctttttg aagggggtga taaagtgaag 60 ttggcgattt tgaaagcgat agaggtgggt tacagataca ttgatacagc tgccgtgtac 120 cgaactgaag agtctgttgg tgaagctgta gcagaagcac ttcaacttgg cttgataaaa 180 tctcgagacg agctattcat aacctccaag ctatggggtg tcgatgctca tcctgatctt 240 gtcctcccag ctcttcagaa ctcattgagg aaacttaaat tggagtattt ggatctgtat 300 ctgatacact atccggtaag cttgaagcca ggggagatgg tcgatgatat accgaaggac 360 gaaatatttc cactggacta caagtctgta tgggcagcca tggaagagtg tcagaagctt 420 ggctacacta agtcaatcgg ggtcagtaat ttctcttgca aaaagcttca acagcttatg 480 gccacagcta acatacctcc agctgtaaat caagtggaga tgaacccaac ttggcaacaa 540 aagaatctaa gggaatactg taaggccaac aatatcttca ttacagcata ctcgactttg 600 ggagctaaag accttttatg gggctccaat gcagttttag gctctaaagt gttgaaccag 660 attgccgtgg ccagaggaaa atcagttgcc caggttagtc tgagatgggt ttacgaacaa 720 ggtgtgagtc ttgtggtgaa aagttttaac gaggagagga tgaaggagaa cttgaagata 780 tttgattggg aactgaccac agaagacttg aaaatgatca gtgagatccc tcaacgcaga 840 gtagcaactg cggatttttt tgtgtcagat attggaccct tcaaatcttt agaagagctg 900 tgggatgaat aa 912 <210> 42 <211> 921 <212> DNA <213> Papaver Bracteatum <400> 42 atgccggttt taggactggg aacagctgaa aaccttacta aaggttctga aagagagatg 60 ctggcgattt tgaaggcgat agaggtgggt tacagacact ttgatacagc tttcatatac 120 caaactcaag agtgtgttgg tgaagctata gctgaagcac ttcaactagg tatcatcaaa 180 tctcgggatg aactctttat cacttccaag ctctggggtt ctgatgctca ccctgattgc 240 gtcctcctgg ctcttcagaa ttctctaagg aatcttaaac tgcagtatct ggatctatat 300 ctgatacact atccagtaag cttgaagcca gggacaactt tgaaagattt ggggaataag 360 gacaacttcc ttccaatgga ctacaagtct gtatgggcag ctatggaaga gtgtcagaag 420 cttggcctca ctaagtcgat aggtgtcagc aacttctcct ccaaaaagat tcaagagcta 480 atgagcacag acagcatccc tcctgccgta aatcaagtag agatgaaccc cacatggcaa 540 caaaagaaac tgagagaata ttgtcaggcc aacaatatct tagttactgc atactctcct 600 ttgggagcta aaggaaccac atggggatcc aatgcggtta tgggatctga ggtactgaac 660 cagattgccc ttgccagagg gaaatctgtt gctcagatta gtctaagatg ggtttacgag 720 caaggtgtga gtctcgtggt gaaaagcttc aatgaagaga ggatgaggga gaacctgaaa 780 atatttgact gggagctaac tgccgaagac ttgaaaaaga tagatgagct tccacagagc 840 agagtggcaa ctgctgaatt tgttgtatca gagaatgggc ccttcaagtc tttagaagaa 900 ttttgggatg acgagtcctg a 921 <210> 43 <211> 966 <212> DNA <213> Papaver Bracteatum <400> 43 atggagaatg caatacccgc ggtaacattg aattctggaa gtgtgatgcc tgtattaggt 60 atgggaactg ctgcataccc atttgtcgaa tcagaagaag cgaaattggc gattttgaat 120 gctatcaaga ctggttacag acatttagat actgctgctc tttaccagtc tgaagaatca 180 cttggtgaag ctgtatctga agcaattcaa cttggtttga taaaatctcg agacgaactg 240 tttatcactt ctaagctctg gccctgtgat gctcaccctg accttgtcat ccccgcaatt 300 cagaactctc taaggaatct aaagttggag taccttgatc tatatctgat acattttcca 360 ataagcacga aaccagctgc agggcttgtt tttccgccac caaaggatgc tttgcttcca 420 atggattaca agtctgtatg ggcagctatg gaagagtgtc aaaaacttgg tcttacaaag 480 tcaattggtg tcagcaactt ctcttgtaag aagcttcaaa ccattttgga cattgctaac 540 atccctccag ctgttaacca agtggagatg aacccagttt ggcagaactt gcaattgagg 600 gacttctgca aggataagag tatcattctc actgcctact caccattaga agggaaggga 660 accccgtggg ggtctaatgc agtctacgga gcacaagttt tgcatgaaat tgctgaggct 720 aaaggcaaaa ctcacgcaca ggtttgtctg agatgggtat atgagcaagg agtgagtctg 780 ctagtgaaga gcttcaatga tcagaggatg aaggaaaaca tgatgatttt cgattgggaa 840 ctgaccgagg atgaactgga aaagataagc agaatcccac agaggagagg actcccaggt 900 gatttttttg tctcggaagc agcgcctttc aagactgtcg aagaattttg ggacggagaa 960 atttga 966 <210> 44 <211> 966 <212> DNA <213> Papaver Bracteatum <400> 44 atggagaatg caatacccgc ggtaacattg aattctggaa gtgtgatgcc tgtattaggt 60 atgggaactg ctgcataccc atttgtcgaa tcagaagaag cgaaattggc gattttgaat 120 gctatcaaga ctggttacag acatttagat actgctgctc tttaccagtc tgaagaatca 180 cttggtgaag ctgtagctga agcaattcaa cttggtttaa taaaatctcg agacgaactt 240 tttatcactt ctaagctatg gccttgtgat gcacaccctg accgtgtcgt ccctactctt 300 cagaactctc taaggaagtt aaagttggag taccttgatc tatatctaat acattggcca 360 gtaagctcga acccagtggc agggcatgtg ttatcgctgc caaaggattc tttggttaca 420 atggattacg agtctgtatg ggcagctatg gaagagtgtc aaaaacttgg tcttacaaag 480 tcaattggtg tcagcaactt ctcttgtaag aagcttcaaa ccattttgga cattgctaac 540 atccctccag ctgttaacca agtggagatg aacccagttt ggcagaactt gaaattgagg 600 gacttctgca aggctaacaa tatcgttctc actgcctact cacctttagg agccagggga 660 accccgtggg ggtctaatgc agtctatgag gagcgagttc tgcatgaaat tgctgaggct 720 aaagggaaaa ctcatgcaca ggtgtgtcta agatgggtat acgagcaagg agtgagtctt 780 atagtcaaga gcttcaatga gttgaggatg aaggagaata tgatgatttt cgactgggaa 840 ctgactgaag atgaactgca aaagataggc aaaatcccac agaggagagg actcccaggt 900 gatttttttg tctcggaagc agcgcctttc aagactgtcg aagaattttg ggacggagaa 960 atttga 966 <210> 45 <211> 972 <212> DNA <213> Papaver Bracteatum <400> 45 atggagaatg taatacctgc agtgacatta agttctggaa gtgtgatgcc tatattggct 60 atgggcactg ctgcataccc attagttgaa ccagaagaag cgaaattggc gtttttgaat 120 gctatcaaga ttggttacag acattttgat acagctgctt cttaccattg tgaaggattt 180 cttggtgaag ctatagctga agcacttcaa cttggtttga taaaatctcg agacgaactg 240 tttatcactt ctaagctctg gccctgtgat gctcaccctg accttgtcat ccccgcaatt 300 cagaactctc taaggaatct aaagttggag taccttgatc tatatctgat acattttcca 360 ataagcacga aaccagctgc agggcttgtt tttccgccac caaaggatgc tttgcttcca 420 atggattaca agtctgtatg ggcagctatg gaagagtgtc aaaaacttgg tcttacaaag 480 tcaattggtg tcagcaactt ctcttgtaag aagcttcaaa ccattttgga cattgctaac 540 atccctccag ctgttaacca agtggagatg aacccagttt ggcagaactt gcaattgagg 600 gacttctgca aggataagag tatcattctc actgcctact caccattaga agggaaggga 660 accccgtggg ggtctaatgc agtctacgga gcacaagttt tgcatgaaat tgctgaggct 720 aaaggcaaaa ctcacgcaca ggtttgtctg agatgggtat atgagcaagg agtgagtctg 780 ctagtgaaga gcttcaatga tcagaggatg aaagaaaaca tgatgatttt cgattgggaa 840 ctcactgaag atgaactgga aaagataggc aaaatcccac agagtagagg actcccaggt 900 gatgtttttg tctcggaact tgaagctgcc cctttcaaga ctgtcgaaga attctgggac 960 ggagaggttt ga 972 <210> 46 <211> 960 <212> DNA <213> Sanguinaria canadensis <400> 46 atggagaacg tctctgtagt gactttgaac tcaggaagag agatgccaat tctaggtatg 60 ggcaccgctg catacccttc tgttggctca gaagaagcca aattggcgat tcttcttgcg 120 ataaaggtcg gctacagaca ctttgacaca gctgctgctt accagattga gtcttctctt 180 ggcgaagctg tagctgaagc acttcaactt ggactgctca aatctcgaga tgaactcttc 240 atcacctcca agttatggtg ctctgattct caccctgaac gtgtcctccc cgctcttcag 300 aactccctcc ggaatcttaa gttggagtat cttgatctat atctgataca ttggccggta 360 agcttgaagc cagggaactt tgatctgact atacccaaag aggatttgct ccctatggac 420 tacaagtctg tgtgggcagc catggaagaa tgtcagaaac ttggcctcac aaagtcgatt 480 ggtgtcagca atttctcttg caagaagctt caagatttat tggccaccgc caacatccct 540 cccgctgtta atcaagtgga gatgaaccca atttggcaac agaagaaatt gagggagttc 600 tgcaaggcca acggtatcct tattacagcc tactcaccgt tgggagccaa tggaactcct 660 tggggatcca gcggagtcgc caatactgag gtgctacacc aaattgctaa ggctagaggg 720 aagactcatg ctcaggtttg tctaaggtgg gtatacgagc aaggagtgag tttgttagta 780 aagagcttca atgaagagag gatgaaggag aacctgaaga tattcgattg ggaactgact 840 gcagaagatt tgaaacagat cagcgagatc ccacagcatc gaggactccc tgctgatatt 900 ttcgtctcag ttaacgggcc cttcaagact gtcgaagagt tctgggatgg cgaggtttga 960 <210> 47 <211> 966 <212> DNA <213> Sanguinaria canadensis <400> 47 atggagatta gtgttccaat aacgactctg agctccggga gggagatgcc aatcttaggt 60 atgggaactg ctgaaaatct attcaatgga tccgaaaaag tgaaattggc aattctagat 120 gcgataaaag tgggatacag acactttgat acagctgctg tgtaccaaac tgagtcgtct 180 ttgggtgaag cagtagctga agcacttcaa catggactgc tcaaatctcg agacgaactc 240 ttcatcacct ccaaattatg gtgctctgat tctcaccctg atcgtgtcct ccctgctctt 300 caaacctctc tcaggaagct taagttggag tatcttgatc tatatctgat acattggccg 360 ttaagctcta agcctgggag ccatgattat cctataccca aggaggattt gctccctttg 420 gactacaagt ctgtgtgggc agccatggaa gaatgtcaga agcttggcct cacaaagtcg 480 attggtgtca gcaatttctc ttgcaagaag cttcaagatt tattggacac agccaacatc 540 cctcccgctg ttaatcaagt ggagatgaac ccgctttggc aacagaagaa actgctggag 600 ttttgtaagg ggaacggtat tatcatcact gccttttcgc cgttgggtgc caaaggcacc 660 tcctggggcg ccaccaacgg agttatggac tctgaggtgc tacaccagat tgcccaggct 720 agaggaaagt ctattgctca ggtttctctg aggtggttat acgagcaagg tgtgagtctg 780 gttgtgaaga gtttcaatgt agagaggatg aaggagaacc tgaagatatt cgattgggaa 840 ttaagcgcag aagacttaaa aaagatcaac gagatcccac agcgtagagg actccctagt 900 ggtagtttca tctcagctaa cgggccattc aagtctgaag aagagctctg ggacggagaa 960 gtctga 966 <210> 48 <211> 957 <212> DNA <213> Sanguinaria canadensis <400> 48 atggccatgg aaaccgttcc taagctaccc ttaagctccg gtgacaattc catcccggta 60 ataggtttcg gcaccgccgc gtttccactc cccgatgacg aaactttaaa atcggcgttt 120 ctcaatggga ttgaagccgg ttaccgtcac tttgacacgg cggctgccta tggttcggag 180 aaggctctcg gcgaagctat aaccgaagct ttacgtctcg gtctgctaaa atctcgagaa 240 gaagttttca tcaccactaa gctttggtgt agcagttgtg aacgcagcct cgtggttcct 300 tcactcaaga atagcctccg gaatctacaa atggagtacg tagatctttt cctcatacat 360 tggcctgtaa gattaagtat tgatgcacag cgacctccaa ttccaaggga acaaattctt 420 actttcgata ctaaatcagt ttgggaagga atggaagaat gtcacgaact tggcctcgcc 480 aaaaatatcg gacttagtaa tttttatccc aagaagatcg atgaattgct cgccaccgca 540 aaaattcccc cggccgtcct tcaagtggaa ttgagcccaa cttggcaaca gaagaatttg 600 atcgaatatt gtagggaaaa gggtatcctt gtcacggctt attctgcttt aggagccaac 660 ggcacgcatt ggggagacaa tagagtggtg gagagcgatg tattaggaga catcgccaag 720 gctagaggaa aaaccactgc tcagattgcg ttgagatggg tgtatgaaca aggggtgtgt 780 atggtggtga agagcttcaa caaggaaagg atgaaactaa acctccagat ctttgatttt 840 gagttgaccg aggaagagtc aaacaagatc agtcaacttc cacaaatcaa aggagttaaa 900 ctatctccca tgtttgggaa ccatgatgta ctcaaggagc ttgaggatca actctga 957 <210> 49 <211> 987 <212> DNA <213> Sanguinaria canadensis <400> 49 atgggtgagg aaatgaagtg tggtagggtt gttccagtgg cgactctgaa ctccggaagg 60 acgatgccgc tcttaggtat gggaacagcc gcatatccat ttgtcgggtc ggaaaaggta 120 aagtctgcga ttcttcatgc gataaagctg gggtaccggc actttgatac cgctgcgctg 180 taccaaactg aggagtgtct tggtgaagtt gtagctgaag cacttcaact tggactgctc 240 aaatctcgag acgaactctt catcacttcc aagctatggt gctctgattc tcaccctgac 300 cgtgtcctcc ctgctcttca gacctccctt cggaagctta agttggagta ccttgatcta 360 tatctgatac attggccgct aagtttgaag cctgggaact acggatttcc catacccaaa 420 gaggacatgc tccctatgga ctacaagtct gtgtgggcag ccatggaaga atgtcaaaag 480 cttggcctca caaagtcgat cggtgtcagc aatttctctt gcaagaagct tcaagattta 540 ttggccacag ccaacatccc tcccgctgtt aatcaagtgg agatgaaccc aatttggcaa 600 caaaagaaac tgagagagtt ttgcgaggcc aatggtatcc ttattacagc ctactcgcct 660 ttaggagcca atggaacccc ttggggctcc agcggagtcg cccagactca ggtgctgcac 720 gacattgcta aggctagagg gaagactcat gctcaggttt gtgtgagatg ggtatacgag 780 caaggggtga gtgtgttggt gaaaagctac aatgaagaga ggatgaagga gaacctgacg 840 gtattcgatt gggaattgga cgtagaggac ctgaagaaga tcagtactga aatcccacag 900 cgtagaggac ttcctagtga cattttcgtt tcagtactta ctggaccctt caagtctgca 960 gaagagctct gggatggaga gctctga 987 <210> 50 <211> 981 <212> DNA <213> Stylophorum diphyllum <400> 50 atggagagtg atagtacagc agtagcagtt ccagttacga ctctgagctc aggtattgag 60 atgccaatgt taggtctggg aactgctgat gaaaaacttc ttccaagttc cgaaacagtg 120 aagttggctt ttctgactgc gataaaacta gggtacaggc acttcgacac agctgctgtg 180 taccaaactg aggagtgtct tggtgaagct gtagctgaag cacttcaact tggtctgatc 240 aaatctagag acgaactctt catcacttcc aagctgtggt gctctgatgc tcaccctgat 300 cttgtcatcc ctgctcttca gaactctctc cggaagctga agttggagta tcttgattta 360 tatctgatac attggccgtt aagctcaaag ccagggaact gtgtttttcc aatacccaag 420 gaggacctcc ttcctttgga cttcaagtct gtgtgggcag ccatggaaga atgtcaaaag 480 cttggcctca caaagtcaat tggtgttagc aacttctctt gcaagaagct tcaagattta 540 ctggccatgg ctatcatccc gcccgctgtt aatcaagtgg agatgaaccc acattggcaa 600 cagaagaaac tgagggagtt ttgtaaggtc aaagatattg tcgttacagc ctactcgcct 660 ctgggagcca aaggcacccc atggggctcc aacgcagtta tggactccaa ggtgctacat 720 cagattgcca aggctagagg gaagtctgtt gctcaggttt ctcttagatg ggtataccag 780 caaggtgtgg ttttgttggt gaagagtttc aatgaagaaa ggatgaagga gaacatgaag 840 atattcaatt gggaactgaa cgaagaagac ttaaaaatga tcgacgagat cccacaatgt 900 agaggcaacc ctagtcatta ttacgtctca gaaaacggac cattcaagac tgttgaagaa 960 ttctgggatg gagaagtcta a 981 <210> 51 <211> 924 <212> DNA <213> Stylophorum diphyllum <400> 51 atgccaattg ttggtatggg aacagctgaa aacctttttg aaggttcaga aagagtgaag 60 ttggctcttc tgactgcaat aaaggtgggt tatagacact ttgatacagc tgctgtttac 120 cagactgagg ggtctcttgg tgaagctata gctgaagcac ttcaacttgg actgatcaaa 180 tctagagacg aactctttat aacttccaag ctgtggattc atgattgtca ccatgatctt 240 gtcctccctg ctcttcagaa ttctcttagg aagcttaagt tggagtacct tgacctctat 300 ttgatacatt ggccagtaag ctcgaagcca ggggagctca ggtctcttat accaaaggag 360 gagctcctcc caatggattt caagtctgtg tgggtagcca tggaagaatg tcacaagatt 420 ggcctcacaa agtcaattgg tgttagcaac ttctcttgca agaagcttca agatttattg 480 ctcactgcca acatccctcc tgctgttaac caaaaggtgg agatgaaccc actttggcaa 540 cagaagaaac tgagggagtt ttgtaaggcc aacggtatcg tcattgcagc ttactcgcct 600 ttaggagcca aaggaacctt gtggggaacc aacggtgtta tggactctga ggtgctacaa 660 cagattgcca aggcaagacg aaagtctatt gcgcaggttt ctctacgatg ggtatatgaa 720 caaggggtgg ttttgttggt gaagagtttt aatgaaggga ggatgaagga aaacctgaag 780 atattcgatt gggaattaag tagagaagac ttgaaacaaa tcaacgagat cctcccacag 840 cgtagaggac tccctagtca tgtttttgtc tccgatgacg ggccattcaa gtctgaggaa 900 gaactgtggg atggagaagt gtga 924 <210> 52 <211> 957 <212> DNA <213> Stylophorum diphyllum <400> 52 atgaccatgg aagctgttcc aaaggtaacc ttaagcacca gtggcaaatc catgcccata 60 attggtttcg gcaccgcctt gtttccgact ggcaatgacg aagccgtgaa atcagcggtt 120 ctaagtggga ttgaagctgg ttatcgtcac ttcgacacag cagtcgccta cagatccgaa 180 aagggtctcg gtgaaggtat agcagaagct ctacgtctag gtctgatctt atctcgagac 240 gaagttttca tcaccaccaa gctttggtgt actagttgcg agcgcagcct cgttgtacct 300 tcactcaaga acagcctccg aaatctacaa atggaatacg tagatctttt tctcatacat 360 tggcctttac gactaagtgc cgatgcacaa cgacctccga ttcgaaacga tcagatactt 420 catttcgata cgaaatcagt ttgggaagga atggaagaat gttatgaact aggcctcgcc 480 aaaaatatag gagttagtaa cttctatccc aagaagattg atgagttact cgccactgca 540 aaaattcctc cagcaatcaa tcaagtggaa ttaaacccag cttggaacca gaaggatctg 600 atcaagtatt gtaagtcaaa gggtattctt atcacggctt actctgcttt aggagccaac 660 ggaacgcatt ggggagataa ccgagttgtg gacagcgatg tgctgaagga catcgcgaaa 720 gctcgaggaa aatccacagc tcaggttgca ttgagatggg tatatgagca aggggtgagt 780 atggtggtca agagctttaa caaggaaagg atgaaacaga accttcagat cttcgatttt 840 gagttgacgg aggaagagtc aaacaagatc agtcaacttc cacaacacaa aggtgttaaa 900 cttactccag tgtatggcga ccatgatgcc atgaagaaga ttgacaatga gatctga 957 <210> 53 <211> 960 <212> DNA <213> Tinospora cordifolia <400> 53 atggtgagtg tacctgaggt ggttctgagc aacgggcacc tcatgcctgt ggttggtatg 60 ggcatggcgg cctacccatt ccaagaatct gaaacagtga agcaggcaat gatcagggca 120 attaagatgg gctacaggca ttttgacaca gctgccttgt accaaacaga gaagtccctt 180 ggagatgcaa ttgtagaagc actaaagctt ggcctcatta aatctcgtga cgagcttttc 240 atcacttcca agctgtggtg cagtgatgct catccccatc acgtcattcc ggctcttcaa 300 agaactctga ggaatcttgg gttggagtac cttgatctct atctcataca ctggccagta 360 agctcagaac ctggcaaata cgagttccca gtacgaaagg aagagctaat tctgatggat 420 tttgaaaatg tatgggcaga tatggaagaa ggatataggc ttggcctcac aaagtcaatt 480 ggtgtaagca acttctcttg taagaagctc gaaagtttac tcactatggc aaagatccct 540 cctgcagtga atcaagtgga gatgaatccc ctctggagac agaataaact actaaggttt 600 tgtaaggaca agggcattgc tatcactgct tactcaccac tgggagccaa aggaactata 660 tggggcacta acagagtgat ggaaagcgag gtgctaaacg atactgccaa tgctagagaa 720 aagactcttg ctcaggtttg tctaagatgg gtttatgagc aagaagtatc catattggtc 780 aaaagcttca acgaagagag gatgaaggag aacttagaca tttttgattg gaaattaagt 840 gacgaggact tggaaaagat caatgagatt ccacaatgca gaggacttcc aggtgatatc 900 tttgtatcag atgatgggcc cttcaaatct gaagcagagc tctgggatgg agaaatctga 960 <210> 54 <211> 990 <212> DNA <213> Tinospora cordifolia <400> 54 atggtgggag ttaacaagga gaacgtggtt tcatcagtag caacggtgcc ggtggtgacg 60 ctgaactccg gtcataaaat gccagtgctg ggaacgggca cagcttcgtt tcctgttcct 120 ccattagagg aactgaagaa ggtgatcatg gaagccatgg aagtgggcta ccgccacttt 180 gatacagcag ccatgtatca aagcgaggaa gggctcggtg cggccattaa agaggcctta 240 gagaagggtc taattaagag cagagatgag cttttcatca ctaccaaact ttggtgcaat 300 aatgcccaac cccatcttgt gctccctgcc atccgcgact ctctcaggag gctaaggttg 360 gaatatgtgg acttatattt gattcattat ccagtaaggc tgaaggaaga tttattgagt 420 atggactgca aggaggatga aatatttccc attgacataa aatcagtatg gtcggcaatg 480 gaggaaatac acaatcttgg tcttgcaaaa tccattggag tcagcaactt cacttgcaag 540 aagctcactg atctacttgc tcatgcaaag atccctcctg cggttaatca ggtggaattg 600 cacccagcat ggcaacaaaa aaaattaaga gaattctgcc aggagaaagg catccaagtc 660 agtgcatatt ctcctcttgg agctaagcaa tggggatttg atgttgttct tagcaataag 720 atcatcaaag agattgccca tcacaaagga aaaacggttg ctcagattgc tttaaggtgg 780 ggacatgagc aaggaataat tttaatccca aagagcttca acaaggaaag attgattcag 840 aatctcctca tatttgattg ggagctaact caagatgaac tgaagaagat ggcatcaatt 900 caacagtcta gaatagccat agcccctgag tttgtgttcc ctggcagccc tttcaaatcc 960 tttgaagagt tctgggatgg agaaatgtga 990 <210> 55 <211> 966 <212> DNA <213> Tinospora cordifolia <400> 55 atgggaatta gcatgcctga cgcaaccctt aattccggcc atcggattcc tctcatcgga 60 ttcggaaccg cgagtttccc cttccctctt gaaggatcag aaactgctac acgggcaata 120 ctggaagcca tcaagattgg ttaccggcac tttgacacgg ctgccttgta ccagacagag 180 gttggacttg gtgaagcaat agaagaagct cttcatcttg gtctcatcga gtcccgtgat 240 gagctcttca ttacctccaa gctttggtgc actcatggcc gccctgagcg cgtccttcct 300 gcaattcgag aatctcttaa gaatctgaag ctggactacc ttgatcttta tctgatacac 360 atgccaatga gcttcaagtc agaaaagcca tggttcccat tacctggaga gtttgaagcc 420 atggatttca agtctgtatg ggaacagatg gaagcatgcc agaggcttgg ccttgcaaga 480 tccattggag ttagcaactt ctcctgcaaa aagctccaac tcatcctcac tactgcaaat 540 attcctcctg ctgtgaatca agtggagatg aacgcccttt ggcaacagaa taagctgaga 600 gagttttgca agagtgaagg cattgttatc actgcttact ctcccttggg aggcaaagga 660 accagttggg gttcaaatag agttatggaa tgtgaagtga taaaagagat tgccacggaa 720 aaaggaaaaa cacacgctca ggtttgtctt agatggctgt acgagcaagg cgtgagcatg 780 gtggtgaaga gcttcaacaa agagagaatg aaagagaact tggagatatt cgactgggag 840 ctcacaagta aagagtgtga gcagatcaag aagctaccgc aaagcagagg atttactgca 900 caagggttgg tctcagaaga tgggcctttc aagtctatag aagatatatg ggatggagag 960 atttga 966 <210> 56 <211> 1002 <212> DNA <213> Thalictrum flavum <400> 56 atgaggaggc cacacagcag actagctagt actagagata tgagtagtat tgttcctgat 60 gtggttctta gctctggcca taaaatgcct cttattggct tcggcacagt tgcgtatcct 120 atcgcagcat cagactccat aaagacagct atagtcaatg gaattaagca tggttacaga 180 cactttgata ctgcttcagt ttaccaaaca gaacaactac ttggtgaagc tatagctgaa 240 gcacttcaat ttggtcttat taaatctaga caagaacttt tcataacttc taagctatgg 300 tgcagtgatt ctcatcatga tcgtgttctc cctgctctgc agaatactct caagactctt 360 cagctagatt accttgatct gtatctcgta cattggccaa taagctctaa accagggaag 420 cacgagttcc caataccgaa agaggaactt cttcccctgc attttgagtc tgtatggaca 480 gccatggaag agtgtcaggc tgttggcctt acaaaatcga ttggtgtcag taacttttct 540 tccaagaagc ttgagcagct actctccact tccaagattc cacctgcggt taatcaagtg 600 gagatgaatc caatttggca acaatataag ttgcgagagt tttgtaaatc caaaggtata 660 gttattactg ccttttctcc attgggtgcc aaaggaacgt cttggggaac taataaagtt 720 atggactcgg aggtgttgaa tgagattgct caggctaaag gaaagactca tgctcaggtt 780 tgtcttcgat ggttgcatga acaaggcata tgtgtggtag tgaaaagctt caatgaagaa 840 aggatgaagg agaacctgga gatattcgac tgggaattga gcccagagga gtccgcatta 900 atccagcaat taccgcagag tagaggaaat actggagagg actttatctc agtcgacggg 960 ccattcaagt ccttagaaga gctatgggat ggagagattt ga 1002 <210> 57 <211> 963 <212> DNA <213> Thalictrum flavum <400> 57 atgggaagta tacctgaggt aagcttaaac tccggtcacc ggatgccact cataggcatg 60 ggcacagcat catacccttt tgcaggatca gaagtagtaa agtcagctat actcagtgca 120 atcaaacttg gtaacagaca ctttgacact gctgctttat acaaaacaga gcaaattatt 180 ggagaagcta tagctgaagc acttcaactt ggtcttatca aatctagaga agagcttttc 240 attactacca agctatgggt gaatgatggt cacaaagatt gcatcctccc tgctcttcaa 300 acatctctca aaaacctgca gctggactac gttgatctat atcttataca ttggccagta 360 tgcatcccac caggggagtt gcgaatgcct ggaccaaatg aaaaggttct tcccatagat 420 tatgcatctg tatgggaagc aatggaagag tgtcaaaggc atggccttac aaaatcaatt 480 ggagtcagca atttcacctg taagaaactt gaattgatac tggcctcagc aaagatccca 540 ccggctgtta atcaggtgga ggtgaatcca ctatggagac aagaaaaagt gataaggttt 600 tgcaaggaca gaggcattgc tgtcacagct tactctcctt tgggcactgc tggatcagat 660 tttatgggca acagaaacgt ggtgcaaagt gagttactga aagagattgc caaagctaca 720 ggaaagacgc atgctcaggt ttgtataaga tgggtttttg agcaaggagt aggggtaata 780 gttaagagct tcaaggagat gagactggag gagaacattg acatatttga ttgggaacta 840 agcaaagaag actctctgaa aattagccga ttaccagaga gtaaaggcta tcccggacat 900 ggcttcatta gagttgaagg gccattcaaa tctcttgagg agctttggga tggagagatc 960 tga 963 <210> 58 <211> 963 <212> DNA <213> Xanthoriza simplicissima <400> 58 atgggtagtg ttccagagat aaccttgaac tccggccaca gtatgcctgt cttaggcttg 60 ggtaccgcat ctgttccctt tgctggatat gaagtagtga agtcagcgat actcagtgcg 120 attaagcttg gttatagaca ctttgataca gcagcgttat acagaacaga gcagcctcta 180 ggagatgcta tagctgaagc aattcgcctt ggcatcatca aatccagaga agagcttttt 240 attacctcca gaccatggct caatgatact caccacgatc gaatcctccc cgctcttcag 300 acgactctcc aaaatctcca actggattac cttgatcttt atcttataca ttggccactg 360 agcataaaac cagggaatat acgattccct ggaccagacg aaaagattct tccaatggat 420 ttcgagtctg tatgggcagc catggaagag tgccaaaggc ttggtctcac aaggtcgatt 480 ggagtcagta atttctcctg caagaaactt gagaagattc ttgcctcagc aacgatccct 540 cctgctgtta atcaagtgga ggtgaaccca ctatggcgac aagagaaact gatacagttt 600 tgcaagtcta aaggtattgt tataacagct tattctcctt tgggaactgt tggttcaagt 660 tttcaaggta acaacaatat catggaatgt gaggttctga aagagattgc agggaacaga 720 ggaaagactc atgctcaggt ttgtcttaga tgggtatatg agcaaggggt ttgtttgctg 780 gtaaagagct tcaatgaggc gaggctgaag gaaaacatgg aaatatttgg ctgggcattg 840 agtgaggaag aagcaaacca gatcaatcag ctgccacagg gtagaggata tcctggacat 900 aatttcatta cgcctgatgg gcctttcaaa tctgaagagg agctttggga tggagagata 960 taa 963 <210> 59 <211> 325 <212> PRT <213> Argemone Mexicana <400> 59 Met Glu Glu Val Ile Gly Leu Glu Thr Val Val Pro Lys Val Thr Leu 1 5 10 15 Ser Ser Ser Ser Gly Asp Asn Ser Met Pro Val Ile Gly Phe Gly Thr 20 25 30 Ala Ala Ser Pro Met Ala Asp His Glu Ile Thr Lys Ala Ala Val Leu 35 40 45 Val Gly Ile Glu Asn Gly Tyr Arg His Phe Asp Thr Ala Ala Ala Tyr 50 55 60 Gly Thr Glu Lys Ala Val Gly Glu Ala Ile Ala Glu Ala Leu Arg Leu 65 70 75 80 Gly Leu Ile Lys Ser Arg Ala Glu Val Phe Ile Thr Thr Lys Leu Trp 85 90 95 Cys Arg Ser Cys Glu Arg Asp Leu Val Val Pro Ser Leu Lys Asn Ser 100 105 110 Leu Gln Asp Leu Gln Thr Asp Tyr Val Asp Leu Phe Leu Ile His Trp 115 120 125 Pro Val Arg Leu Arg His Asp Ala Gly Arg Pro Pro Ile Ser Arg Asp 130 135 140 Gln Ile Leu Thr Phe Asp Thr Lys Ser Val Trp Glu Gly Met Glu Glu 145 150 155 160 Cys His Glu Leu Gly Leu Ala Lys Asn Ile Gly Val Ser Asn Phe Ser 165 170 175 Ile Lys Lys Leu Glu Glu Leu Leu Ala Thr Ala Lys Ile Pro Pro Val 180 185 190 Leu Asp Gln Val Glu Leu Ser Pro Thr Trp His Gln Lys Asn Leu Ile 195 200 205 Glu Tyr Cys Lys Glu Lys Gly Ile His Val Thr Ala Tyr Ser Ala Leu 210 215 220 Gly Ala Asn Asn Thr His Trp Gly Asn Asn Arg Val Val Glu Ser Asp 225 230 235 240 Val Leu Gly Glu Ile Ala Lys Ala Arg Gly Lys Thr Thr Ala Gln Ile 245 250 255 Ala Leu Arg Trp Val Tyr Glu Gln Gly Val Ser Met Val Val Lys Ser 260 265 270 Phe Asn Gln Glu Arg Met Lys Gln Asn Leu Gln Ile Phe Asp Phe Glu 275 280 285 Leu Thr Glu Glu Glu Ser Asn Lys Ile Ser Gln Phe Pro Gln His Lys 290 295 300 Gly Val Ser Leu Ser Ala Val Tyr Gly Asp His Asp Ile Leu Lys Glu 305 310 315 320 Met Glu Ala Glu Leu 325 <210> 60 <211> 322 <212> PRT <213> Argemone Mexicana <400> 60 Met Glu Asn Val Ile Pro Arg Val Thr Leu Ser Ser Gly Ser Val Met 1 5 10 15 Pro Ile Leu Gly Met Gly Thr Ala Ser Tyr Pro Phe Val Ser Ser Glu 20 25 30 Glu Gly Lys Leu Ala Ile Leu Asn Ala Ile Lys Val Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ser Thr Tyr Met Phe Glu Glu Phe Leu Gly Glu Ala 50 55 60 Ile Ala Glu Ala Ile Gln Leu Gly Leu Ile Lys Ser Arg Lys Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Cys Cys Asp Ala His Pro Asp Leu Val 85 90 95 Val Pro Ser Leu Arg Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Val Ser Leu Lys Ala Gly Arg Tyr 115 120 125 Glu Tyr Pro Pro Leu Lys Glu Ala Leu Val Leu Met Asp Tyr Lys Ser 130 135 140 Val Trp Ala Ala Met Glu Glu Cys Gln Asn Leu Gly Leu Thr Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu Asp 165 170 175 Tyr Ala Lys Ile Pro Pro Ser Val Asn Gln Val Glu Met Asn Pro Val 180 185 190 Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys Ala Asn Asp Ile Val 195 200 205 Ile Thr Ala Tyr Ser Pro Leu Gly Ala Ser Gly Thr Pro Trp Gly Ser 210 215 220 Ser Arg Val Leu Asp Ala Gln Val Leu His Glu Ile Ala Arg Ala Lys 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln Gly 245 250 255 Val Ser Leu Leu Val Lys Thr Phe Asn Glu Glu Arg Met Lys Glu Asn 260 265 270 Leu Arg Ile Phe Asp Trp Glu Leu Thr Glu Glu Asp Lys Gln Lys Ile 275 280 285 Ser Lys Ile Pro Gln His Arg Gly Leu Thr Gly Asp Ala Phe Val Ser 290 295 300 Glu His Glu Gly Ala Pro Phe Lys Thr Ala Glu Glu Leu Trp Asp Gly 305 310 315 320 Glu Val <210> 61 <211> 322 <212> PRT <213> Argemone Mexicana <400> 61 Met Asp Met Asn Lys His Ala Val Val Tyr Val Pro Thr Lys Val Ile 1 5 10 15 Met Gly Ile Pro Val Ile Gly Phe Gly Thr Ser Ala Asp Pro Pro Val 20 25 30 Asp Phe Asp Thr Thr Arg Leu Ala Ile Met Gln Ala Ile Glu Asn Gly 35 40 45 Tyr Arg His Phe Asp Thr Ala Ala Leu Tyr Asn Ser Glu Gln Pro Leu 50 55 60 Gly Glu Ala Ile Asn Glu Ala Ile Ser Arg Gly Leu Ile Lys Ser Arg 65 70 75 80 Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp Thr His Pro 85 90 95 Gln His Ile Leu Pro Ala Ile Gln Lys Thr Leu Arg Asn Leu Asn Met 100 105 110 Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser Arg His 115 120 125 Glu Asn Phe Glu Tyr Pro Ile Lys Lys Glu Asp Phe Leu Pro Met Asp 130 135 140 Phe Lys Lys Val Trp Ala Ala Met Glu Glu Cys His Lys Gln Gly Leu 145 150 155 160 Ala Lys Phe Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Asp Asn 165 170 175 Ile Met Ala Ser Ala Thr Ile Ser Pro Ser Val Leu Gln Val Glu Val 180 185 190 Asn Pro Cys Trp Gln Gln Lys Arg Leu Ile Asp Phe Cys Lys Ala Asn 195 200 205 Gly Ile Phe Val Val Ala Tyr Ala Pro Leu Gly Ala Val Gly Thr Ile 210 215 220 Tyr Gly Thr Asn Arg Val Met Glu Ser Asp Val Leu Lys Gln Ile Ala 225 230 235 240 Lys Lys Arg Gly Lys Ser Val Ala Gln Val Cys Leu Arg Trp Ala Tyr 245 250 255 Glu Gln Gly Ile Gly Val Val Val Lys Ser Phe Asn Lys Glu Arg Met 260 265 270 Lys Gln Asn Leu Glu Ile Phe Asp Trp Lys Leu Ser Glu Glu Asp Thr 275 280 285 Lys Lys Ile Ser Glu Ile Gln Gln Asp Arg Ala Cys Leu Gly Met Asp 290 295 300 Tyr Thr Ser Pro Tyr Gly Pro Tyr Lys Thr Ile Lys Gln Leu Trp Asp 305 310 315 320 Glu Glu <210> 62 <211> 322 <212> PRT <213> Berberis thunbergii <400> 62 Met Glu Ile Val Pro Lys Val Thr Leu Asn Ser Gly Tyr Gln Met Pro 1 5 10 15 Leu Leu Gly Phe Gly Thr Ala Glu Asn Trp Pro Phe Thr Lys Ser Glu 20 25 30 Ala Ala Lys Arg Ala Ile Leu Cys Ala Ile Lys Asn Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ser Met Tyr Gln Ser Glu Glu Ser Leu Gly Glu Ala 50 55 60 Ile Val Glu Ala Leu His Leu Gly Leu Ile Lys Ser Arg Asn Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp Cys His Gln Asp Arg Val 85 90 95 Leu Pro Ala Leu Arg Glu Thr Leu Lys Asn Leu His Leu Asp Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Trp Pro Leu Ser Ala Lys Pro Gly Lys His 115 120 125 Glu Tyr Pro Ile Leu Lys Asp Lys Leu Leu Pro Met Asp Tyr Glu Ser 130 135 140 Val Trp Gly Ala Met Glu Glu Cys Gln Arg Leu Gly Leu Ser Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Ser Lys Lys Leu Glu Gln Leu Leu Lys 165 170 175 Thr Ala Lys Ile Ile Pro Ala Val Asn Glu Val Glu Val Asn Pro Val 180 185 190 Trp His Gln Asn Lys Leu Ile Glu Phe Cys Lys Ala Lys Gly Ile Val 195 200 205 Val Thr Gly Tyr Ser Pro Leu Gly Ala Lys Gly Thr Thr Trp Gly Thr 210 215 220 Asn Lys Val Met Asp Ser Glu Val Leu Lys Glu Ile Ala Lys Thr Arg 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Ile Tyr Glu Gln Gly 245 250 255 Val Gly Leu Val Ala Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn 260 265 270 Leu Glu Ile Phe Asp Trp Ala Leu Thr Glu Glu Glu Ser Lys Gln Ile 275 280 285 Ser Gln Leu Pro Gln Tyr Lys Gly Gln Asn Gly Glu Thr Phe Leu Ser 290 295 300 Ala Glu Gly Pro Phe Lys Thr Leu Glu Glu Leu Trp Asp Glu Glu Val 305 310 315 320 Ala Ala <210> 63 <211> 320 <212> PRT <213> Berberis thunbergii <400> 63 Met Glu Ile Val Pro Lys Val Thr Leu Asn Ser Gly Tyr Gln Met Pro 1 5 10 15 Leu Leu Gly Phe Gly Thr Ala Glu Asn Trp Pro Phe Thr Lys Ser Glu 20 25 30 Ala Ala Lys Arg Ala Ile Leu Cys Ala Ile Lys Asn Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ser Met Tyr Gln Ser Glu Glu Ser Leu Gly Glu Ala 50 55 60 Ile Val Glu Ala Leu His Leu Gly Leu Ile Lys Ser Arg Asn Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp Cys His Gln Asp Arg Val 85 90 95 Leu Pro Ala Leu Lys Asn Thr Leu Gln Asn Leu Gln Leu Asp Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Leu Ser Ser Lys Arg Gly Lys Leu 115 120 125 Glu Tyr Pro Leu Gln Glu Asp Glu Thr Tyr Pro Met Asp Phe Gly Ser 130 135 140 Val Trp Glu Glu Met Glu Lys Cys Gln Arg Val Gly Leu Thr Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Ala Lys Lys Ile Glu His Leu Leu Thr 165 170 175 Thr Ala Lys Ile Ile Pro Ala Val Asp Gln Val Glu Met Asn Pro Leu 180 185 190 Trp Gln Gln Arg Thr Leu Arg Glu Tyr Cys Lys Thr Lys Gly Ile Val 195 200 205 Val Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Ala Ile Trp Gly Ser 210 215 220 Asn Ile Val Met Glu Asn Glu Val Leu Ala Glu Ile Ala Lys Ala Lys 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Leu Arg Ser Val Tyr Glu Lys Gly 245 250 255 Val Ala Leu Val Thr Arg Ser Tyr Asn Glu Glu Arg Met Lys Gln Asn 260 265 270 Leu Asn Ile Phe Asp Trp Asn Leu Ser Lys Glu Glu Thr Lys Arg Ile 275 280 285 Asp Asn Leu Pro Gln Lys Arg Val Phe Leu Gly Glu Gly Phe Leu Ser 290 295 300 Pro Leu Gly Pro Phe Lys Ser Pro Glu Glu Leu Trp Asp Gly Glu Met 305 310 315 320 <210> 64 <211> 324 <212> PRT <213> Corydalis chelantifolia <400> 64 Met Met Val Ser Val Pro Glu Val Thr Leu Gln Ser Ser Ser Ser Asp 1 5 10 15 Lys Lys Met Pro Ile Met Gly Met Gly Thr Ala Ala Tyr Pro Phe Ala 20 25 30 Glu Ser Asp Glu Ala Lys Ala Ala Leu Leu Thr Ser Ile Lys Leu Gly 35 40 45 Tyr Arg His Phe Asp Thr Ala Ser Leu Tyr His Asn Glu Lys Ser Val 50 55 60 Gly Lys Ala Ile Ser Glu Ala Leu Lys Leu Gly Leu Val Lys Ser Arg 65 70 75 80 Asp Glu Phe Tyr Ile Asn Ser Lys Leu Trp Cys Ser Asp Ser His Pro 85 90 95 Asp Arg Val Ile Pro Ala Leu Gln Glu Ser Leu Lys Lys Leu Gly Leu 100 105 110 Glu Tyr Leu Asp Met Tyr Leu Ile His Trp Pro Leu Ser Leu Lys Lys 115 120 125 Gly Ser Tyr Glu Phe Pro Ile Pro Thr Asp Glu Glu Ile Leu Pro Met 130 135 140 Asp Phe Lys Ala Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly 145 150 155 160 Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu 165 170 175 Thr Ile Leu Ser Thr Ala Lys Ile Pro Pro Val Val Asn Gln Val Glu 180 185 190 Leu Asn Thr Leu Trp Gln Gln Thr Lys Leu Asn Lys Phe Cys Lys Asp 195 200 205 Asn Gly Ile Val Leu Met Ala Phe Ser Pro Leu Gly Ala His Gly Ser 210 215 220 Pro Trp Gly Thr Asn Arg Val Met Glu Cys Glu Leu Leu His Glu Ile 225 230 235 240 Ala Lys Ala Lys Gly Lys Thr Leu Ala Gln Val Cys Leu Arg Trp Ile 245 250 255 Tyr Glu Gln Gly Ala Gly Ile Leu Val Lys Ser Tyr Asn Glu Val Arg 260 265 270 Met Lys Glu Asn Leu Glu Ile Phe Asp Trp Glu Leu Ser Ala Glu Glu 275 280 285 Leu Lys Ser Ile Ser Glu Leu Pro Gln His Arg Gly Phe Pro Ala Asp 290 295 300 Ser Leu Leu Ser Ala Lys Gly Pro Phe Arg Thr Glu Glu Glu Leu Trp 305 310 315 320 Asp Gly Glu Ile <210> 65 <211> 339 <212> PRT <213> Corydalis chelantifolia <400> 65 Met Glu Ser Val Ala Cys Glu Pro Gly Val Ile Glu Gln Gln Gln Tyr 1 5 10 15 Phe Ile Pro Glu Val Thr Cys Gly Gly Gly Ser Ser Thr Thr Arg Met 20 25 30 Arg Met Pro Val Val Gly Met Gly Thr Ala Ser Tyr Pro Phe Ala Gly 35 40 45 Ser Glu Gly Val Lys Gln Ala Leu Leu His Ala Ile Lys Leu Gly Tyr 50 55 60 Trp His Phe Asp Thr Ala Ser Phe Tyr Gln Thr Glu Gln Ser Leu Gly 65 70 75 80 Glu Ala Ile Ser Glu Ala Ile Gln Leu Gly Leu Ile Lys Ser Arg Asp 85 90 95 Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Lys Asp 100 105 110 Leu Val Leu Pro Ala Leu Gln Asn Thr Leu Arg Asn Leu Gln Leu Glu 115 120 125 Tyr Leu Asp Leu Tyr Leu Ile His Phe Pro Ile Ser Leu Lys Pro Gly 130 135 140 Arg Tyr Asp Phe Pro Val Gln Ser Lys Glu Asp Leu Leu Pro Met Asp 145 150 155 160 Phe Lys Ser Ile Trp Ala Lys Met Glu Glu Cys Gln Gly Leu Gly Leu 165 170 175 Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Asn 180 185 190 Leu Leu Ala Thr Ala Lys Ile Pro Pro Ala Val Asn Gln Val Glu Met 195 200 205 Asn Pro Leu Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys Ala Asn 210 215 220 Gly Ile Thr Ile Thr Ala His Ser Pro Leu Gly Ala Lys Gly Thr Asn 225 230 235 240 Trp Gly Thr Asn Arg Val Val Glu Cys Glu Val Leu His Gln Ile Ala 245 250 255 Lys Ala Lys Gly Lys Ser His Ala Gln Val Cys Leu Arg Trp Val Tyr 260 265 270 Glu Gln Gly Val Ser Leu Leu Val Lys Ser Phe Asn Lys Glu Arg Met 275 280 285 Lys Glu Asn Leu Gly Ile Phe Asp Trp Glu Leu Asn Ala Glu Asp Leu 290 295 300 Lys Lys Ile Ser Glu Ile Pro Gln Gly Arg Gly Leu Pro Val Asp Gly 305 310 315 320 Phe Leu Ser Pro Asn Gly Pro Phe Lys Ser Glu Glu Glu Phe Trp Asp 325 330 335 Gly Glu Ile <210> 66 <211> 301 <212> PRT <213> Corydalis chelantifolia <400> 66 Met Pro Val Val Gly Phe Gly Thr Ala Lys Phe Pro Phe Gly Asp Asp 1 5 10 15 Glu Ala Ile Lys Leu Ala Val Leu Glu Gly Ile Lys Leu Gly Tyr Arg 20 25 30 His Phe Asp Thr Ala Thr Lys Tyr Lys Ser Glu Lys Pro Leu Gly Glu 35 40 45 Thr Ile Val Glu Ala Ile Asn Leu Gly Leu Ile Lys Ser Arg Asp Glu 50 55 60 Leu Phe Ile Thr Ser Lys Leu Trp Cys Gly Ser Thr Glu Arg Ser Leu 65 70 75 80 Ile Val Pro Ser Leu Lys Thr Ser Leu Gln Asn Leu Gln Leu Asp Tyr 85 90 95 Leu Asp Leu Tyr Leu Ile His Trp Pro Leu Arg Phe Ser Thr Asp Glu 100 105 110 Thr Pro Thr Pro Val Pro Lys Glu Gln Leu Leu Pro Phe Asp Thr Lys 115 120 125 Ser Val Trp Gln Ala Met Glu Glu Cys Gln Asn Leu Gly Leu Thr Lys 130 135 140 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Glu Leu Leu 145 150 155 160 Ser Thr Ala Lys Ile Thr Pro Ala Val Asn Gln Val Glu Leu Asn Thr 165 170 175 Ser Trp Gln Gln Thr Lys Met Arg Glu Phe Cys Lys Gly Lys Gly Ile 180 185 190 His Ile Thr Gly Tyr Ser Pro Leu Gly Ser Tyr Gly Thr Lys Trp Gly 195 200 205 Asp Asn Arg Val Val Glu Asn Gln Val Leu Glu Glu Ile Gly Lys Pro 210 215 220 Arg Gly Lys Thr Ala Val Gln Val Cys Leu Arg Trp Ile Tyr Glu Gln 225 230 235 240 Gly Ala Ser Met Val Val Lys Ser Phe Asn Lys Glu Arg Met Lys Ala 245 250 255 Asn Leu Asp Ile Phe Asp Trp Lys Leu Thr Glu Glu Glu Leu Lys Arg 260 265 270 Ile Ser Gln Leu Pro Gln His Lys Ala Ser Leu Pro Thr Ala Ser Phe 275 280 285 Gly Asp His Asp Glu Val Arg Glu Leu Asp Tyr Glu Ile 290 295 300 <210> 67 <211> 327 <212> PRT <213> Chelidonium majus <400> 67 Met Glu Ser Asp Ser Ala Ala Ala Ala Val Val Val Pro Val Thr Thr 1 5 10 15 Leu Ser Ser Gly Ile Glu Met Pro Met Leu Gly Met Gly Thr Val Glu 20 25 30 Asn Phe Leu Pro Gly Ser Glu Thr Val Lys Leu Ala Leu Leu Asn Ala 35 40 45 Ile Lys Leu Gly Tyr Arg His Phe Asp Thr Ala Ala Ile Tyr Gln Thr 50 55 60 Glu Glu Cys Leu Gly Glu Ala Val Ala Glu Ala Leu Gln Leu Gly Leu 65 70 75 80 Ile Lys Ser Arg Asp Gln Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser 85 90 95 Asp Ala His Pro Asp Leu Val Ile Pro Ala Leu Gln Asn Ser Leu Arg 100 105 110 Lys Leu Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Val His Trp Pro Leu 115 120 125 Ser Ser Lys Pro Gly Asn Cys Val Phe Pro Ile Leu Lys Glu Asp Leu 130 135 140 Leu Pro Leu Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln 145 150 155 160 Lys Leu Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys 165 170 175 Lys Leu Gln Asp Leu Leu Thr Ser Ala Ile Ile Pro Pro Val Val Asn 180 185 190 Gln Val Glu Met Asn Pro Arg Trp Gln Gln Lys Lys Leu Arg Glu Phe 195 200 205 Cys Lys Ala Arg Asp Ile Val Val Thr Ala Tyr Ser Pro Leu Gly Ala 210 215 220 Lys Gly Thr Val Tyr Gly Ser Gly Ala Val Met Asp Cys Glu Val Leu 225 230 235 240 His Gln Ile Ala Lys Ile Arg Gly Lys Ser Val Ala Gln Val Ser Leu 245 250 255 Arg Trp Val Tyr Gln Gln Gly Val Thr Pro Leu Val Lys Ser Phe Asn 260 265 270 Glu Glu Arg Met Lys Glu Asn Met Lys Ile Phe Asp Trp Glu Leu Tyr 275 280 285 Glu Glu Asp Leu Lys Met Ile Asp Glu Ile Pro Gln Ser Arg Val Asn 290 295 300 Pro Cys Tyr Phe Phe Leu Ser Glu Asn Gly Pro Phe Lys Thr Val Glu 305 310 315 320 Glu Phe Trp Asp Gly Glu Val 325 <210> 68 <211> 327 <212> PRT <213> Chelidonium majus <400> 68 Met Glu Ser Ser Asn Asn Ala Val Leu Val Pro Val Ile Thr Leu Asn 1 5 10 15 Ser Gly Arg Glu Met Pro Ile Val Gly Met Gly Thr Ala Glu Asn Leu 20 25 30 Phe Gln Gly Ser Glu Arg Val Lys Leu Ala Leu Leu Thr Ala Ile Lys 35 40 45 Val Gly Tyr Arg His Phe Asp Thr Ala Ala Val Tyr Gln Thr Glu Glu 50 55 60 Ser Leu Gly Glu Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys 65 70 75 80 Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Leu Pro Asp Cys 85 90 95 His His Asp Leu Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Lys Leu 100 105 110 Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser 115 120 125 Lys Pro Gly Glu Ile Lys His Val Ile Pro Lys Glu Glu Leu Leu Pro 130 135 140 Met Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys His Lys Leu 145 150 155 160 Gly Leu Ala Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu 165 170 175 Gln Asp Leu Leu Val Thr Ala Asn Ile Pro Pro Asp Val Asn Gln Val 180 185 190 Glu Met Asn Pro Leu Trp Gln Gln Thr Lys Leu Arg Glu Phe Cys Lys 195 200 205 Ala His Gly Ile Leu Val Ala Ala Tyr Ser Pro Leu Gly Ala Lys Gly 210 215 220 Thr Ala Trp Gly Arg Thr Asn Gly Val Met Asp Ser Glu Val Leu Gln 225 230 235 240 Gln Ile Ala Lys Ala Arg Gly Lys Ser Ile Ala Gln Val Ser Leu Arg 245 250 255 Trp Val Tyr Glu Gln Gly Val Val Leu Leu Val Lys Ser Phe Asn Glu 260 265 270 Glu Arg Met Lys Glu Asn Leu Lys Ile Phe Asp Trp Glu Leu Ser Gly 275 280 285 Glu Asp Leu Lys Lys Ile Ser Glu Ile Leu Pro Gln Arg Arg Gly Leu 290 295 300 Pro Ser His Val Phe Val Ser Asp Asp Gly Pro Phe Lys Ser Glu Glu 305 310 315 320 Glu Leu Trp Asp Gly Glu Val 325 <210> 69 <211> 327 <212> PRT <213> Chelidonium majus <400> 69 Met Glu Ser Ser Asn Asn Ala Val Leu Val Pro Val Ile Thr Leu Asn 1 5 10 15 Ser Gly Arg Glu Met Pro Ile Val Gly Met Gly Thr Ala Glu Asn Leu 20 25 30 Phe Gln Gly Ser Glu Arg Val Lys Leu Ala Leu Leu Thr Ala Ile Lys 35 40 45 Val Gly Tyr Arg His Phe Asp Thr Ala Ala Val Tyr Gln Thr Glu Glu 50 55 60 Ser Leu Gly Glu Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys 65 70 75 80 Ser Arg Asp Glu Leu Phe Val Thr Ser Lys Leu Trp Ile Pro Asp Cys 85 90 95 His His Asp Leu Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Lys Leu 100 105 110 Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser 115 120 125 Lys Pro Gly Glu Leu Arg Ser Leu Ile Pro Lys Glu Glu Leu Leu Pro 130 135 140 Met Asp Phe Lys Ser Val Trp Val Ala Met Glu Glu Cys His Lys Leu 145 150 155 160 Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu 165 170 175 Arg Asp Leu Leu Leu Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val 180 185 190 Glu Met Asn Pro Leu Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys 195 200 205 Ala Asn Gly Ile Val Ile Ala Ala Tyr Ser Pro Leu Gly Ala Lys Gly 210 215 220 Thr Ala Trp Gly Arg Thr Asn Asp Gly Val Met Asp Ser Glu Leu Leu 225 230 235 240 Gln Lys Ile Ala Lys Ala Arg Gly Lys Ser Thr Ala Gln Val Ser Leu 245 250 255 Arg Trp Gly Tyr Glu Gln Gly Val Ile Leu Leu Val Lys Ser Phe Asn 260 265 270 Glu Glu Arg Met Lys Glu Asn Leu Lys Thr Phe Asp Trp Glu Leu Ser 275 280 285 Gly Glu Asp Leu Lys Gln Ile Asn Glu Ile Leu Pro Gln Arg Arg Gly 290 295 300 His Ser His Glu Phe Ile Ser Asp Asn Gly Pro Phe Lys Ser Glu Glu 305 310 315 320 Glu Leu Trp Asp Gly Glu Val 325 <210> 70 <211> 328 <212> PRT <213> Chelidonium majus <400> 70 Met Gly Glu Glu Met Ile Lys Thr Ser Ser Val Glu Ile Pro Val Ala 1 5 10 15 Thr Leu Ser Thr Gly Arg Thr Met Pro Leu Leu Gly Met Gly Thr Ala 20 25 30 Ala Tyr Pro Phe Val Gly Ser Asp Gly Val Val Lys Ala Ile Leu His 35 40 45 Ala Ile Lys Leu Gly Tyr Arg His Phe Asp Thr Ala Ala Leu Tyr Phe 50 55 60 Thr Glu Glu Cys Leu Gly Asp Ala Val Ala Glu Ala Leu Arg Leu Gly 65 70 75 80 Leu Ile Lys Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Tyr Cys 85 90 95 Cys Asp Ala His Pro Asp Arg Val Val Pro Ala Leu Gln Asn Ser Leu 100 105 110 Arg Lys Leu Lys Met Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro 115 120 125 Ala Ser Thr His Glu Gly Asn Phe Glu Phe Pro Leu Gln Lys Gln Asp 130 135 140 Ile Leu Pro Leu Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys 145 150 155 160 Gln Lys Leu Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys 165 170 175 Lys Lys Leu Gln Asp Leu Leu Ala Ser Ala Lys Ile Pro Pro Ala Val 180 185 190 Asn Gln Val Glu Met Asn Pro Ile Trp Gln Gln Asn Lys Leu Arg Glu 195 200 205 Phe Cys Lys Ala Asn Asp Ile His Ile Thr Ala Tyr Ser Pro Leu Gly 210 215 220 Ala Asn Gly Thr Pro Trp Gly Ser Ser Gly Val Ala Glu Thr Gln Val 225 230 235 240 Leu His Asp Ile Ala Lys Ala Arg Gly Lys Thr His Ala Gln Val Cys 245 250 255 Leu Arg Trp Val Tyr Glu Gln Gly Val Ser Val Leu Val Lys Ser Tyr 260 265 270 Asn Glu Glu Arg Met Lys Glu Asn Leu Ala Val Leu Asp Trp Glu Leu 275 280 285 Ser Glu Glu Asn Leu Lys Lys Ile Ser Glu Ile Pro Gln Arg Arg Gly 290 295 300 Leu Pro Gly Asp Ile Phe Val Ser Asp Asn Gly Pro Phe Lys Thr Val 305 310 315 320 Glu Glu Leu Trp Asp Gly Glu Val 325 <210> 71 <211> 306 <212> PRT <213> Cocculus trilobus <400> 71 Met Pro Val Val Gly Met Gly Leu Ala Ala Tyr Pro Phe Gln Glu Ser 1 5 10 15 Glu Val Val Lys Leu Ala Met Leu Arg Ala Ile Glu Met Gly Tyr Arg 20 25 30 His Phe Asp Thr Ala Ala Leu Tyr Gln Thr Glu Gln Ser Leu Gly Leu 35 40 45 Ala Ile Ala Glu Ala Leu Glu Leu Gly Leu Ile Lys Ser Arg Asp Glu 50 55 60 Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp Ala His Pro His Leu 65 70 75 80 Val Ile Pro Ala Leu Gln Arg Thr Leu Lys Asn Leu Gly Leu Asp Tyr 85 90 95 Val Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser Asn Lys Pro Gly 100 105 110 Lys Tyr Asp Phe Pro Val Pro Lys Glu Glu Leu Val Gln Met Asp Tyr 115 120 125 Glu Ser Val Trp Ala Ala Met Glu Glu Gly His Arg Leu Gly Leu Thr 130 135 140 Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Asn Leu 145 150 155 160 Leu Thr Ile Ala Lys Val Pro Pro Thr Val Asn Gln Val Glu Met Asn 165 170 175 Pro Phe Trp Arg Gln Tyr Lys Leu Lys Glu Phe Cys Lys Glu Lys Gly 180 185 190 Ile Ile Ile Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr Ile Trp 195 200 205 Gly Thr Asn Lys Val Met Glu Ser Lys Val Leu Glu Asp Ile Gly Ile 210 215 220 Ala Ser Gly Lys Thr Leu Ala Gln Val Cys Leu Arg Trp Val Tyr Glu 225 230 235 240 Gln Gly Val Thr Leu Leu Val Lys Ser Phe Asn Glu Glu Arg Met Lys 245 250 255 Gly Asn Leu Asp Ile Phe Asp Trp Glu Leu Ser Glu Lys Glu Leu Lys 260 265 270 Lys Ile Asn Glu Ile Pro Gln Ser Arg Gly Leu Pro Gly Asp Ile Phe 275 280 285 Val Ser Asp Asp Gly Pro Phe Lys Ser Glu Ala Glu Leu Trp Asp Gly 290 295 300 Glu Val 305 <210> 72 <211> 321 <212> PRT <213> Eschscholzia californica <400> 72 Met Gly Ser Cys Ser Ile Pro Val Lys Ser Leu Ser Ser Gly Arg Glu 1 5 10 15 Met Pro Val Ile Gly Met Gly Thr Ala Asp Ser Asn Leu Ala Gly Ser 20 25 30 Asp Ala Ser Arg Ile Ala Leu Val Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 His Phe Asp Thr Ala Ser Ile Tyr Gln Ser Glu Gln Asn Leu Gly Asp 50 55 60 Ala Ile Ala Gln Ala Leu Glu Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Thr Ser Lys Leu Trp Ser Ser Asp Ser His Pro Asp Arg 85 90 95 Val Val Pro Ala Leu Gln Glu Ser Leu Arg Lys Leu Lys Leu Glu Tyr 100 105 110 Leu Asp Leu Tyr Leu Ile His Trp Pro Ala Ser Ser Thr Pro Gly Ile 115 120 125 Tyr Glu Phe Pro Ile Pro Lys Asp Glu Ile Phe Pro Leu Glu Tyr Glu 130 135 140 Thr Ile Trp Ala Ala Met Glu Glu Cys Gln Arg Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Ser Lys Lys Leu Gln Thr Ile Leu 165 170 175 Asn Thr Ala Thr Ile Pro Pro Ala Ala Asn Gln Val Glu Met Asn Pro 180 185 190 Val Trp Gln Gln Lys Lys Leu Lys Glu Phe Cys Asp Ala Asn Asn Ile 195 200 205 Leu Leu Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr Ser Trp Gly 210 215 220 Ser Asn Lys Val Met Glu Ser Glu Val Leu Lys Gln Ile Ala Met Ala 225 230 235 240 Asn Gly Lys Ser Val Ala Gln Val Ser Leu Arg Trp Leu Tyr Gln Ile 245 250 255 Gly Ala Thr Met Val Val Lys Ser Tyr Asn Ala Glu Arg Met Lys Glu 260 265 270 Asn Leu Lys Ile Phe Asp Trp Glu Leu Ser Asp Glu Asp Met Lys Ala 275 280 285 Ile Ser Glu Ile Pro Gln Arg Arg Asn Phe Ala Gly Asp Ile Leu Ile 290 295 300 Ser Ser Asn Gly Pro Phe Lys Ser Glu Asp Glu Leu Trp Asp Gly Glu 305 310 315 320 Val <210> 73 <211> 323 <212> PRT <213> Eschscholzia californica <400> 73 Met Ser Ser Tyr Gly Ser Val Pro Met Lys Ser Leu Asn Ser Gly Ser 1 5 10 15 Lys Ile His Val Leu Gly Phe Gly Thr Ala Ala Tyr Pro Phe Val Gly 20 25 30 Ser Asp Gly Val Lys Asn Ala Ile Leu Asn Ala Ile Lys Leu Gly Tyr 35 40 45 Arg His Phe Asp Thr Ala Ala Leu Tyr Phe Thr Glu Glu Ser Leu Gly 50 55 60 Glu Ala Val Ser Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp 65 70 75 80 Glu Leu Phe Ile Thr Ser Lys Leu Trp Ile Ser Asp Ala Tyr Pro Asp 85 90 95 Arg Val Leu Pro Ala Ile Gln Lys Thr Leu Arg Asn Leu Lys Met Glu 100 105 110 Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Ile Ser Gly Lys Glu Gly 115 120 125 Asn Phe Asp Leu Pro Val Pro Lys Asp Cys Leu Gln Glu Leu Asp Tyr 130 135 140 Lys Pro Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr 145 150 155 160 Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Ile Ile 165 170 175 Leu Ser Ser Ala Thr Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn 180 185 190 Pro Ile Trp His Gln Lys Lys Leu Arg Glu Phe Cys Lys Ala Asn Asn 195 200 205 Ile Met Ile Ala Ala Tyr Ser Pro Leu Gly Ala Ala Gly Thr Pro Trp 210 215 220 Gly Ser Ser Gly Val Val Glu Thr Glu Val Leu His Glu Ile Ala Arg 225 230 235 240 Ser Arg Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu 245 250 255 Gln Gly Val Ile Val Leu Val Lys Ser Phe Asn Glu Glu Arg Met Lys 260 265 270 Glu Asn Leu Ser Val Phe Asp Trp Glu Leu Thr Asp Glu Asp Leu Lys 275 280 285 Lys Ile Ser Gln Ile Pro Gln Arg Arg Gly Leu Pro Ser Gly Asp Ile 290 295 300 Trp Val Ser Thr Glu Gly Pro Phe Arg Ser Glu Glu Glu Leu Trp Asp 305 310 315 320 Gly Glu Ile <210> 74 <211> 324 <212> PRT <213> Glaucium flavum <400> 74 Met Glu Met Ser Ser Ala Gln Val Val Pro Leu Met Thr Leu Asn Ser 1 5 10 15 Gly Lys Glu Ile Pro Val Leu Gly Met Gly Thr Ala Glu Asn His Phe 20 25 30 Gln Gly Ser Asp Gly Thr Lys Asp Ala Leu Val Asn Ala Ile Lys Ile 35 40 45 Gly Tyr Arg His Phe Asp Thr Ala Ala Ile Tyr Asn Val Glu Glu Cys 50 55 60 Leu Gly Asp Ala Ile Ala Glu Ala Leu Gln His Gly Leu Ile Lys Ser 65 70 75 80 Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Ile Ser Asp Ser His 85 90 95 Pro Asp Arg Val Leu Phe Ala Leu Gln Asn Ser Leu Arg Lys Leu Lys 100 105 110 Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Leu Ser Ser Thr 115 120 125 Pro Gly Lys Ser Val Phe Pro Val Pro Lys Glu Asp Phe Leu Pro Leu 130 135 140 Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly 145 150 155 160 Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln 165 170 175 Asp Leu Leu Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu 180 185 190 Met Ser Pro Leu Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys Val 195 200 205 Asn Gly Ile Leu Val Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr 210 215 220 Phe Trp Gly Ser Asn Glu Ile Met Asp Ser His Val Leu Gln Glu Ile 225 230 235 240 Ala Lys Ala Arg Gly Lys Ser Val Ala Gln Val Thr Leu Arg Trp Val 245 250 255 Tyr Glu Gln Gly Val Ile Leu Leu Val Lys Ser Phe Asn Glu Asn Arg 260 265 270 Met Lys Glu Asn Met Ala Ile Phe Asp Trp Asp Leu Ser Ile Asp Asp 275 280 285 Leu Lys Lys Phe Asp Glu Ile Ser Gln Arg Arg Gly Asn Leu Gly Asp 290 295 300 Phe Phe Val Ser Glu Asn Gly Pro Phe Lys Ser Val Asp Met Val Trp 305 310 315 320 Asp Gly Glu Val <210> 75 <211> 326 <212> PRT <213> Glaucium flavum <400> 75 Met Glu Ser Ser Ser Ser Ser Ala Val Val Val Pro Val Met Thr Leu 1 5 10 15 Asn Ser Gly Lys Glu Met Pro Val Leu Gly Met Gly Thr Ala Glu Asn 20 25 30 Asn Leu Gln Gly Ser Glu Lys Thr Lys Leu Ala Leu Leu Thr Ala Ile 35 40 45 Lys Ala Gly Tyr Arg His Phe Asp Thr Ala Ser Ala Tyr Asn Thr Glu 50 55 60 Glu Ala Leu Gly Glu Ala Val Ala Glu Ala Leu Gln Leu Gly Leu Ile 65 70 75 80 Lys Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp 85 90 95 Ala His Pro Gly Leu Val Val Pro Ala Leu Gln Asn Ser Leu Arg Asn 100 105 110 Leu Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ser 115 120 125 Leu Lys Pro Gly Lys Phe Val Ile Pro Phe Ser Lys Glu Asp Ile Leu 130 135 140 Pro Leu Asp Phe Lys Asn Val Trp Ala Ala Met Glu Glu Cys Gln Lys 145 150 155 160 Leu Gly Leu Ala Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys 165 170 175 Leu Gln Asp Leu Leu Ala Ile Ala Asn Ile Pro Pro Ala Val Asn Gln 180 185 190 Val Glu Met Ser Pro Leu Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys 195 200 205 Lys Val Asn Gly Ile Leu Val Thr Ala Tyr Ser Pro Leu Gly Ala Lys 210 215 220 Gly Thr Phe Trp Gly Ser Asn Glu Ile Met Asp Ser Asp Val Leu Asn 225 230 235 240 Gln Ile Ala Lys Ala Arg Gly Lys Ser Val Ala Gln Val Ser Leu Arg 245 250 255 Trp Val His Glu Gln Gly Val Ile Leu Leu Val Lys Ser Phe Asn Glu 260 265 270 Asn Arg Met Lys Glu Asn Met Ala Ile Phe Asp Trp Glu Leu Ser Asn 275 280 285 Asp Asp Leu Lys Lys Met Asp Glu Ile Ser Gln Arg Arg Gly Pro Leu 290 295 300 Ala Asp Ile Phe Val Ser Glu Asn Gly Pro Phe Lys Ser Val Asp Met 305 310 315 320 Leu Trp Asp Gly Gly Phe 325 <210> 76 <211> 326 <212> PRT <213> Glaucium flavum <400> 76 Met Gly Ser Ser Asp Ala Val Val Val Pro Val Lys Thr Leu Ser Ser 1 5 10 15 Gly Arg Lys Met Pro Val Ile Gly Met Gly Thr Ala Glu Ile Leu Leu 20 25 30 Val Asp Gly Ser Glu Lys Val Lys Leu Ala Leu Leu Asn Ala Ile Lys 35 40 45 Ile Gly Tyr Arg His Phe Asp Thr Ala Ala Val Tyr Arg Thr Glu Glu 50 55 60 Ala Leu Gly Glu Val Val Ala Glu Ala Ile Gln Leu Gly Leu Ile Lys 65 70 75 80 Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Leu Ser Asp Ser 85 90 95 His Pro Asp Leu Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Lys Leu 100 105 110 Lys Phe Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Leu Ser Ser 115 120 125 Lys Pro Gly Glu Leu Lys Val Leu Ile Pro Lys Glu Glu Leu Leu Pro 130 135 140 Leu Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu 145 150 155 160 Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu 165 170 175 Gln Asp Leu Leu Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val 180 185 190 Glu Met Asn Pro Leu Trp Gln Gln Lys Lys Leu Asn Glu Phe Cys Lys 195 200 205 Ala Asn Asp Ile Val Ile Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly 210 215 220 Thr Pro Trp Gly Ser Asn Arg Val Met Asp Ser Glu Val Leu Asn Gln 225 230 235 240 Ile Ala Lys Ala Arg Gly Lys Ser Val Ala Gln Val Ala Leu Arg Trp 245 250 255 Val Tyr Glu Gln Gly Val Ser Leu Val Val Lys Ser Phe Asn Glu Glu 260 265 270 Arg Met Lys Glu Asn Val Thr Lys Ile Phe Asp Trp Glu Leu Ser Ala 275 280 285 Glu Asp Leu Lys Lys Ile Glu Glu Ile Pro Gln Cys Arg Gly Val Pro 290 295 300 Ser His Val Phe Val Ser Val Asn Gly Pro Phe Lys Ser Glu Glu Glu 305 310 315 320 Leu Trp Asp Gly Glu Ile 325 <210> 77 <211> 327 <212> PRT <213> Glaucium flavum <400> 77 Met Gly Glu Glu Met Lys Ile Lys Ser Val Glu Ile Pro Val Val Thr 1 5 10 15 Leu Ser Ser Gly Arg Thr Met Pro Val Phe Gly Met Gly Thr Ala Ala 20 25 30 Phe Pro Phe Val Gly Ser Asp Gly Val Val Lys Ala Ile Thr His Ala 35 40 45 Ile Lys Leu Gly Tyr Arg His Phe Asp Thr Ala Ala Leu Tyr Phe Thr 50 55 60 Glu Glu Ser Leu Gly Asp Ala Ile Ala Glu Ala Leu Arg Leu Gly Leu 65 70 75 80 Ile Lys Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Cys 85 90 95 Asp Ala His Pro Asp Arg Val Leu Pro Ala Leu Arg Asn Ser Leu Arg 100 105 110 Asn Leu Lys Ile Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Ala 115 120 125 Ser Thr His Glu Gly Asn Leu Glu Phe Pro Ile Gln Lys Gln Asp Ile 130 135 140 His Pro Leu Asp Tyr Asn Ser Val Trp Ala Ala Met Glu Glu Cys Gln 145 150 155 160 Thr Leu Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys 165 170 175 Lys Leu Gln His Ile Leu Ala Ile Ala Lys Ile Pro Pro Ala Val Asn 180 185 190 Gln Val Glu Met Asn Pro Ile Trp Gln Gln Lys Lys Leu Arg Glu Phe 195 200 205 Cys Lys Ala Asn Asp Ile His Ile Thr Ala Tyr Ser Pro Leu Gly Ala 210 215 220 Asn Gly Thr Pro Trp Gly Ser Ser Gly Val Val Glu Thr Gln Val Leu 225 230 235 240 His Asp Ile Ala Lys Ala Arg Gly Lys Thr His Ala Gln Val Cys Leu 245 250 255 Arg Trp Val Tyr Glu Gln Gly Val Ile Val Leu Val Lys Ser Tyr Asn 260 265 270 Glu Glu Arg Met Lys Glu Asn Leu Thr Val Phe Asp Trp Glu Leu Ser 275 280 285 Ala Glu Asp Ser Met Lys Ile Ser Glu Ile Pro Gln His Arg Gly Leu 290 295 300 Pro Gly Asp Ile Phe Val Ser Asp Ser Gly Pro Phe Lys Ser Glu Glu 305 310 315 320 Glu Leu Trp Asp Gly Glu Val 325 <210> 78 <211> 319 <212> PRT <213> Hydrastis canadensis <400> 78 Met Gly Ser Val Pro Asn Val Ile Leu Ser Ser Gly His Pro Met Pro 1 5 10 15 Leu Val Gly Phe Gly Thr Ala Gly Phe Pro Phe Gly Thr Ser Glu Gly 20 25 30 Ile Lys Ser Ala Ile Leu Cys Gly Ile Lys Asn Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Val Tyr Gln Thr Glu Gln Ile Leu Gly Glu Ala Ile 50 55 60 Ala Glu Ala Leu Glu Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe 65 70 75 80 Leu Thr Ser Lys Leu Trp Cys Ser Asp Ala His Gln Gln His Val Leu 85 90 95 Pro Ala Leu Gln Lys Thr Leu Arg Thr Leu Gln Leu Asp Tyr Leu Asp 100 105 110 Leu Tyr Leu Val His Trp Pro Leu Ser Ser Lys Pro Gly Lys Tyr Glu 115 120 125 Tyr Pro Ile Pro Lys Glu Glu Leu Leu Pro Met Asp Phe Lys Ser Val 130 135 140 Trp Ala Ala Met Glu Glu Cys Gln Ala Leu Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Gln Leu Leu Ser Thr 165 170 175 Ser Asn Ile Pro Pro Ala Val Asn Gln Val Glu Val Asn Pro Ile Trp 180 185 190 Gln Gln Asn Lys Leu Arg Glu Phe Cys Lys Ala Lys Gly Ile Ile Val 195 200 205 Ala Ala Phe Ser Pro Leu Gly Ala Lys Gly Thr Ser Trp Gly Thr Asn 210 215 220 Lys Val Met Asp Ser Glu Val Leu Asn Glu Ile Ala Gln Ala Arg Gly 225 230 235 240 Lys Thr Thr Ala Gln Asn Cys Leu Arg Trp Leu His Glu Gln Gly Val 245 250 255 Cys Val Val Val Lys Ser Phe Asn Glu Glu Arg Met Lys Gly Asn Leu 260 265 270 Lys Leu Phe Asp Trp Glu Leu Ser Lys Glu Glu Ser Lys Lys Ile Ser 275 280 285 Gln Leu Pro Gln Ser Lys Gly His Thr Gly Asp Asp Met Val Ser Ala 290 295 300 Asn Gly Pro Phe Lys Ser Leu Glu Glu Leu Trp Asp Gly Glu Ile 305 310 315 <210> 79 <211> 319 <212> PRT <213> Hydrastis canadensis <400> 79 Met Thr Arg Ile Pro Glu Ile Val Leu Asn Ser Gly Trp Arg Met Pro 1 5 10 15 Val Leu Gly Met Gly Thr Ala Thr Phe Pro Ile Gln Ser Pro Glu Val 20 25 30 Ile Glu Ser Ser Ile Val Asn Ala Ile Glu Leu Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Val Tyr Gln Ser Glu Ser Pro Leu Gly Arg Ala Ile 50 55 60 Ser Glu Ala Ile Arg Arg Gly Leu Ile Glu Ser Arg Lys Glu Val Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His His Asp Leu Val Ile 85 90 95 Pro Ala Leu His Lys Thr Leu Gln Asn Leu Gly Leu Glu Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Phe Pro Val Arg Leu Lys Gly Asp Ile Ser Phe 115 120 125 Asp Ile Lys Lys Ala Asp Leu Ile Pro Phe Asp Val Lys Gly Thr Trp 130 135 140 Glu Ala Met Glu Lys Cys Gln Glu Leu Gly Leu Thr Arg Ser Ile Gly 145 150 155 160 Val Ser Asn Phe Ser Ser Lys Lys Leu Ser Glu Leu Leu Thr His Ala 165 170 175 Thr Ile Ser Pro Ala Val Asn Gln Val Glu Met His Pro Phe Trp Gln 180 185 190 Gln Lys Glu Leu Arg Ala Phe Cys Ala Asp Lys Gly Ile His Val Ser 195 200 205 Ala Tyr Ser Pro Leu Gly Gly Lys Gly Ala Leu Trp Gly Ser Asp Ile 210 215 220 Leu Leu Asn Ser Lys Glu Ile Glu Arg Ile Ala Gln Ala Lys Gly Lys 225 230 235 240 Ser Ile Ala Gln Val Cys Leu Arg Trp Ala Tyr Glu Gln Gly Val Ser 245 250 255 Tyr Leu Pro Lys Ser Tyr Asn Lys Gly Arg Met Lys Glu Asn Met Glu 260 265 270 Ile Phe Asp Trp Gln Leu Ser Glu Asp Glu Leu Gln Lys Ile Ser His 275 280 285 Leu Pro Gln Gly Lys Ile Tyr Thr Gly His His Phe Ile Ser Asp Asp 290 295 300 Gly Glu Tyr Lys Ser Pro Ile Asp Leu Trp Asp Cys Glu Ile Cys 305 310 315 <210> 80 <211> 320 <212> PRT <213> Hydrastis canadensis <400> 80 Met Gly Ile Val Arg Glu Val Val Leu Asn Ser Gly Glu Arg Met Pro 1 5 10 15 Leu Leu Gly Met Gly Thr Ala Thr Tyr Pro Val Ala Pro Phe Glu Leu 20 25 30 Val Glu Ser Ser Val Ile Ala Ala Ile Glu Leu Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Val Tyr Glu Thr Glu Gln Pro Leu Gly Leu Ala Ile 50 55 60 Ser Glu Ala Val Arg Gln Gly Leu Ile Ala Ser Arg Asp Glu Ile Phe 65 70 75 80 Ile Thr Thr Lys Leu Trp Cys Gly His Ala His Tyr Asp Leu Val Leu 85 90 95 Pro Ala Leu Arg Asp Ser Leu Glu Thr Met Gly Leu Asp Tyr Val Asp 100 105 110 Leu Tyr Leu Ile His Phe Pro Ala Arg Phe Asn Met Lys Glu Lys Ser 115 120 125 Leu Asn Val Asn Lys Lys Asp Leu Leu Pro Leu Asp Ile Arg Gly Thr 130 135 140 Trp Lys Ala Met Glu Glu Cys Tyr Glu Leu Gly Leu Ala Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Ser Gln Leu Leu Ser Val 165 170 175 Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met His Pro Leu Trp 180 185 190 Gln Gln Gln Lys Leu Arg Glu Phe Cys Glu Glu Lys Gly Ile His Val 195 200 205 Ser Ala Tyr Ser Pro Leu Gly Gly Lys Gly Thr Ile Trp Gly Ser Asn 210 215 220 Ala Val Leu Asp Ser Asp Gln Ile Lys Gln Ile Ala Lys Ala Lys Gly 225 230 235 240 Lys Ser Ile Ala Gln Ile Cys Leu Arg Trp Gly Phe Glu His Gly Val 245 250 255 Ser Ile Leu Pro Lys Ser Phe Asn Lys Glu Arg Leu Lys Glu Asn Met 260 265 270 Glu Ile Phe Asp Trp Glu Leu Ser Lys Glu Glu Leu Gln Lys Met Asn 275 280 285 Thr Phe Pro Gln Asn Arg Ile Phe Gln Ala Glu His Leu Val Ser Pro 290 295 300 Asp Glu Gly Leu Phe Lys Ser Val Ala Asp Leu Trp Asp Gly Glu Ile 305 310 315 320 <210> 81 <211> 328 <212> PRT <213> Jeffersonia diphylla <400> 81 Met Cys Phe Cys Val Leu Val Thr Lys Arg Ala Met Gly Ile Val Pro 1 5 10 15 Glu Val Thr Leu Asn Ser Gly His Gln Met Pro Leu Val Gly Phe Gly 20 25 30 Thr Ala Gln Phe Pro Phe Pro Glu Gly Asp Glu Ala Lys Gln Ile Ile 35 40 45 Ala Pro Ile Leu Cys Gly Ile Lys Ser Gly Tyr Arg His Phe Asp Thr 50 55 60 Ala Ser Leu Tyr Lys Thr Glu Glu Ala Leu Gly Glu Ala Ile Lys Glu 65 70 75 80 Ala Leu Arg Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe Ile Thr 85 90 95 Ser Lys Leu Trp Cys Thr Asp Ser His Gln Asp Leu Val Leu Pro Ala 100 105 110 Leu Lys Lys Thr Leu Gln Asn Leu Gln Met Asp Tyr Leu Asp Leu Tyr 115 120 125 Leu Val His Phe Pro Val Ser Ser Lys Pro Gly Lys Pro Glu Phe Pro 130 135 140 Pro Gln Lys Glu Asp Leu Leu Pro Met Asp Phe Gly Ser Val Trp Ala 145 150 155 160 Ala Met Glu Glu Cys Gln Arg Leu Gly Leu Thr Lys Ser Ile Gly Val 165 170 175 Ser Asn Phe Ser Cys Lys Lys Leu Glu Gln Leu Leu Thr Thr Ala Lys 180 185 190 Ile Ile Pro Ala Val Asn Glu Val Glu Met Ser Pro Val Cys Gln Gln 195 200 205 Asn Lys Leu Arg Glu Phe Cys Lys Val Lys Asn Ile Val Val Thr Ala 210 215 220 Tyr Ser Pro Leu Gly Gly Gly Ser Asn Ala Val Lys Asp Asn Lys Val 225 230 235 240 Leu Lys Glu Ile Ala Lys Ala Lys Gly Lys Thr Cys Ala Gln Val Thr 245 250 255 Leu Arg Trp Val Tyr Glu Gln Gly Val Ala Leu Val Pro Lys Ser Phe 260 265 270 Asn Glu Gly Arg Met Lys Glu Asn Leu Asp Ile Phe Asn Trp Thr Ile 275 280 285 Ser Glu Glu Glu Phe Lys Gln Ile Ser Gln Leu Pro Gln Gly Arg Val 290 295 300 Ala Thr Gly Glu Trp Phe Val Ser Val Glu Gly Pro Phe Lys Ser Leu 305 310 315 320 Glu Glu Leu Trp Asp Glu Val Ile 325 <210> 82 <211> 321 <212> PRT <213> Mahonia aquifolium <400> 82 Met Gly Val Val Pro Val Val Thr Leu Asn Ser Gly His Glu Met Pro 1 5 10 15 Leu Val Gly Phe Gly Thr Ala Val Pro Ala Phe Gly Gly Ser Asp Ala 20 25 30 Ile Lys Gln Ala Ile Leu Cys Gly Ile Lys Asn Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Val Tyr Gln Thr Glu Lys Ser Leu Gly Glu Ala Ile 50 55 60 Val Glu Ala Leu Arg Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Gln Asp Gly Val Leu 85 90 95 Pro Ala Leu Arg Glu Thr Leu Lys Asn Leu Gln Leu Asp Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Leu Ser Ala Lys Pro Gly Lys His Glu 115 120 125 Tyr Gln Ile Pro Lys Asp Glu Leu Leu Pro Met Asp Tyr Glu Ser Val 130 135 140 Trp Gly Ala Met Glu Glu Cys Gln Arg Leu Asp Leu Ser Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Ile Lys Lys Leu Glu Glu Leu Leu Lys Thr 165 170 175 Ala Lys Ile Ile Pro Ala Val Asn Glu Val Glu Val Asn Pro Val Trp 180 185 190 His Gln Asn Lys Leu Ile Glu Phe Cys Lys Ala Lys Gly Ile Val Val 195 200 205 Thr Gly Tyr Ser Pro Leu Gly Ala Lys Gly Thr Thr Trp Gly Thr Asn 210 215 220 Arg Val Met Asp Asn Glu Val Leu Lys Glu Ile Ala Lys Thr Arg Gly 225 230 235 240 Lys Thr His Ala Gln Val Cys Leu Arg Trp Ile Tyr Glu Gln Gly Val 245 250 255 Gly Leu Val Ala Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn Leu 260 265 270 Glu Ile Phe Asp Trp Ala Leu Thr Glu Glu Glu Ser Lys Gln Ile Ser 275 280 285 Gln Leu Pro Gln Tyr Lys Gly Gln Asn Gly Glu Thr Phe Leu Ser Ala 290 295 300 Glu Gly Pro Phe Lys Thr Leu Glu Glu Leu Trp Asp Glu Glu Ile Ala 305 310 315 320 Ala <210> 83 <211> 320 <212> PRT <213> Menispermum canadense <400> 83 Met Ala Ser Val Pro Glu Val Thr Leu Ser Ser Gly His Pro Met Pro 1 5 10 15 Leu Phe Gly Phe Gly Thr Ala Ala Trp Pro Phe Val Ser Ser Glu Gly 20 25 30 Ala Lys Ser Ala Ile Leu Cys Gly Met Glu Leu Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Ile Tyr Gly Thr Glu Ser Ser Leu Gly Glu Ala Ile 50 55 60 Ala Glu Ala Leu Lys Leu Gly Ile Ile Lys Ser Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Cys Asp Gly His His Asp Arg Val Leu 85 90 95 Pro Ala Leu Gln Met Thr Leu Lys Asn Leu Gln Leu Asp Tyr Ile Asp 100 105 110 Leu Tyr Leu Glu His Trp Pro Leu Ser Ser Val Pro Lys Gly Glu Leu 115 120 125 Glu Tyr Pro Ile Pro Lys Glu Lys Leu Phe Pro Met Asp Leu Lys Ala 130 135 140 Val Trp Glu Ala Met Glu Glu Cys Gln Arg Leu Gly Leu Thr Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Asp Leu Leu Ala 165 170 175 Phe Ala Lys Ile Pro Pro Ala Val Asn Glu Val Glu Met Asn Pro Val 180 185 190 Trp Gln Gln Lys Lys Leu Arg Asp Phe Cys Lys Ala Lys Gly Ile Val 195 200 205 Leu Thr Ala Tyr Ser Val Leu Gly Ala Lys Gly Thr Leu Trp Gly Thr 210 215 220 Asn Lys Val Met Asp Ser Glu Val Leu Gly Lys Ile Ala Lys Ala Arg 225 230 235 240 Gly Lys Thr Val Gly Gln Val Cys Leu Arg Trp Ala Tyr Glu Gln Gly 245 250 255 Ala Cys Val Leu Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn 260 265 270 Leu Glu Thr Phe Asn Trp Glu Leu Gly Glu Glu Asp Leu Lys Met Ile 275 280 285 Ser Glu Ile Pro Gln Tyr Lys Gly Leu Arg Gly Glu Asp Met Val Ala 290 295 300 Pro Asn Gly Pro Tyr Lys Thr Leu Glu Glu Leu Trp Asp Gly Glu Ile 305 310 315 320 <210> 84 <211> 341 <212> PRT <213> Menispermum canadense <400> 84 Met Gly Val Asp Lys Thr Asn Glu Phe Ser Asn Gly Asn Ala Ala Ser 1 5 10 15 Pro Val Leu Ile Lys Glu Ala Asn Leu Phe Lys Ile Ala Glu Val Thr 20 25 30 Leu Asn Ser Gly His His Met Pro Val Leu Gly Thr Gly Thr Ala Ser 35 40 45 Phe Pro Phe Pro Pro Leu Lys Glu Leu Lys Lys Ala Ile Val Glu Ala 50 55 60 Met Glu Val Gly Tyr Arg His Phe Asp Ser Ala Ala Leu Tyr Gln Ser 65 70 75 80 Glu Glu Gly Leu Gly Ala Ala Ile Ala Glu Ala Leu Glu Lys Gly Leu 85 90 95 Val Lys Lys Arg Glu Glu Leu Phe Ile Thr Thr Lys Leu Trp Cys Asn 100 105 110 Asn Ala His Ser Asp Arg Val Leu Pro Ala Ile Arg Glu Ser Leu Arg 115 120 125 Lys Leu Arg Leu Asp Tyr Val Asp Leu Tyr Leu Ile His Phe Pro Val 130 135 140 Arg Leu Lys Glu Asp Leu Leu Asp Met Asn Cys Lys Lys Gly Gly Ile 145 150 155 160 Phe Glu Leu Asp Leu Lys Ser Val Trp Ala Ala Met Glu Gln Ile Gln 165 170 175 His Leu Gly Leu Ala Lys Ser Ile Gly Val Ser Asn Phe Thr Cys Lys 180 185 190 Lys Leu Thr Asp Leu Leu Ser Tyr Ala Lys Ile Pro Pro Ala Val Asn 195 200 205 Gln Val Glu Met His Pro Val Trp Gln Gln Lys Lys Leu Arg Asp Phe 210 215 220 Cys Lys Glu Lys Gly Ile His Val Ser Ala Tyr Ser Pro Leu Gly Ala 225 230 235 240 Lys Gln Trp Gly Phe Asp Val Val Leu Gly Asn Lys Ile Leu Lys Glu 245 250 255 Ile Ala Gln Asp Lys Gly Lys Thr Ile Ala Gln Ile Ala Leu Arg Trp 260 265 270 Gly Tyr Glu Gln Gly Val Ile Leu Ile Pro Lys Ser Phe Asn Lys Gly 275 280 285 Arg Leu Thr Glu Asn Leu Arg Ile Phe Asp Trp Lys Leu Thr Glu Asp 290 295 300 Glu Leu Lys Lys Ile Ser Ser Ile Gln Gln Ser Arg Val Ala Ile Met 305 310 315 320 Pro Glu Phe Val Phe Pro Glu Ser Pro Phe Lys Thr Phe Glu Asp Phe 325 330 335 Trp Asp Gly Glu Met 340 <210> 85 <211> 322 <212> PRT <213> Menispermum canadense <400> 85 Met Glu Gly Gly Gly Val Pro Lys Ile Leu Leu Asn Ser Gly His Arg 1 5 10 15 Met Pro Val Leu Gly Met Gly Thr Ala Ser Phe Pro Ile Ser Pro Gln 20 25 30 His Leu Val Lys Ser Ser Ile Leu Thr Ala Ile Glu Leu Gly Tyr Thr 35 40 45 His Phe Asp Thr Ala Ser Val Tyr Glu Thr Glu Pro Thr Leu Ser Arg 50 55 60 Ala Ile Arg Gln Ala Leu Glu Gly Arg His Ile Ala Ser Arg Asp Gln 65 70 75 80 Leu Phe Ile Thr Thr Lys Leu Trp Cys Gly Gln Ala Gln Ser Asp Leu 85 90 95 Val Val Pro Ala Leu Arg Glu Ser Leu Gln Glu Leu Gly Leu Asp Tyr 100 105 110 Val Asp Leu Tyr Leu Ile His Phe Pro Ala Arg Phe Lys Cys Val Glu 115 120 125 Lys Thr Phe Asn Val Thr Lys Glu Asp Leu Leu Pro Leu Asp Met Lys 130 135 140 Gly Thr Trp Gln Ala Met Glu Glu Cys Cys Lys Leu Gly Leu Ala Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Ser Gln Ile Leu 165 170 175 Thr Phe Ala Thr Ile Pro Pro Ala Val Asn Gln Val Glu Met His Pro 180 185 190 Leu Trp Gln Gln Arg Lys Leu Arg Glu Phe Cys Lys Glu Lys Gly Ile 195 200 205 His Val Ser Ala Tyr Ser Pro Leu Gly Gly Lys Gly Ala Lys Trp Gly 210 215 220 Ser Asn Ala Val Phe Glu Cys Glu Glu Ile Lys Gln Ile Ala Glu Ala 225 230 235 240 Lys Gly Lys Ser Leu Ala Gln Ile Cys Leu Arg Trp Ala Phe Glu Gln 245 250 255 Gly Val Ser Phe Val Pro Lys Ser Phe Asn Lys Glu Arg Leu Lys Glu 260 265 270 Asn Met Glu Ile Tyr Gly Trp Glu Leu Ser Lys Glu Glu Leu Gln Lys 275 280 285 Leu Ser Val Leu Pro Gln Cys Arg Ile Phe Lys Gly Glu Trp Leu Val 290 295 300 Ser Ala Asp His Gly Val Phe Lys Ser Val Glu Asp Leu Trp Asp Gly 305 310 315 320 Glu Ile <210> 86 <211> 319 <212> PRT <213> Nandina domestica <400> 86 Met Val Ser Val Pro Glu Val Thr Leu Asn Ser Gly His Arg Met Pro 1 5 10 15 Leu Val Gly Met Gly Thr Ala Cys Phe Pro Leu Pro Gly Ser Glu Val 20 25 30 Val Lys Ser Ala Met Val Thr Ala Ile Lys Leu Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ala Leu Tyr Gln Ser Glu Gln Pro Leu Gly Glu Ala Ile 50 55 60 Ala Glu Ala Leu His Leu Gly Leu Ile Ile Ser Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Gln Asp Leu Val Leu 85 90 95 Pro Ala Leu Lys Thr Ser Leu Arg Asn Leu Gln Leu Asp Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Phe Pro Val Ser Leu Lys Pro Gly Lys Ile Gln 115 120 125 Leu Pro Val Pro Lys Glu Glu Leu Leu Pro Met Asp Phe Lys Ser Val 130 135 140 Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu His Ile Leu Ser Leu 165 170 175 Ala Lys Ile Pro Pro Ala Val Asn Gln Val Glu Leu Asn Pro Ile Trp 180 185 190 Arg Gln Lys Lys Leu Ile Glu Phe Cys Lys Ala Lys Gly Ile Val Val 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Ala Val Ala Pro Gly Gln Gly Ser Asn 210 215 220 Arg Val Met Glu Cys Glu Val Leu Ser Glu Ile Ala Lys Ala Arg Gly 225 230 235 240 Lys Thr His Ala Gln Val Cys Leu Arg Trp Ile Ile Glu Gln Gly Val 245 250 255 Ser Leu Val Val Lys Ser Phe Asn Glu Asp Arg Lys Lys Lys Asn Leu 260 265 270 Glu Ile Phe Asp Trp Glu Leu Ser Glu Glu Asp Ser Lys Lys Ile Ser 275 280 285 Gln Ile Pro Glu Thr Arg Gly Asn Pro Gly Asp Phe Val Ile Ser Val 290 295 300 Asp Gly Pro Tyr Lys Ser Arg Glu Glu Leu Trp Asp Gly Glu Ile 305 310 315 <210> 87 <211> 319 <212> PRT <213> Nandina domestica <400> 87 Met Gly Arg Val Pro Glu Thr Thr Leu Ser Ser Gly His Asn Met Pro 1 5 10 15 Leu Leu Gly Met Gly Thr Ala Thr Phe Pro Leu Pro Pro Ser Glu Leu 20 25 30 Ile Glu Ser Tyr Ile Leu Ala Ala Ile Glu Met Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Val Tyr Asp Thr Glu Val Pro Leu Gly Arg Ala Ile 50 55 60 Ser Glu Ala Leu Arg Arg Gly Leu Val Ala Asn Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Thr Lys Leu Trp Cys Gly His Ala His Pro Asp Leu Val Val 85 90 95 Pro Ala Leu His Gln Ser Leu Met Glu Met Gly Leu Glu Tyr Val Asp 100 105 110 Leu Tyr Leu Ile His Phe Pro Ala Arg Phe Asn Lys Lys Glu Lys Asn 115 120 125 Phe Asp Val Lys Lys Glu Glu Val Ile Pro Leu Asp Met Lys Gly Thr 130 135 140 Trp Glu Ala Met Glu Glu Cys Ser Lys Leu Gly Leu Ala Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Ser Gln Leu Leu Thr Tyr 165 170 175 Ala Thr Ile Pro Pro Ala Val Asn Gln Val Glu Met His Pro Leu Trp 180 185 190 Gln Gln Trp Lys Leu Arg Glu Phe Cys Val Ala Asn Asp Ile His Val 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Gly Lys Gly Ala Leu Trp Gly Ser Asn 210 215 220 Ser Val Leu Asp Ser Lys Glu Ile Gln Gln Met Ala Glu Ala Lys Gly 225 230 235 240 Lys Ser Ile Ala Gln Ile Cys Leu Arg Trp Ala Phe Glu Gln Gly Val 245 250 255 Ser Phe Ile Pro Lys Ser Phe Asn Val Glu Arg Leu Lys Glu Asn Met 260 265 270 Glu Ile Phe Asp Trp Glu Met Asn Lys Asn Glu Leu Leu Lys Met Ser 275 280 285 Leu Leu Pro Gln Lys Arg Thr Phe Thr Ala Glu Tyr Leu Val Ser Pro 290 295 300 Asp Gly Ala Phe Lys Ser Val Asp Asp Leu Trp Asp Gly Glu Ile 305 310 315 <210> 88 <211> 319 <212> PRT <213> Nigella sativa <400> 88 Met Ala Arg Val Pro Glu Ile Thr Leu Asn Ser Gly His Arg Met Pro 1 5 10 15 Leu Leu Gly Met Gly Thr Ala Val Val Pro Phe Lys Glu Pro Glu Pro 20 25 30 Val Lys Ser Ala Ile Leu Thr Ala Ile Lys Asn Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ser Leu Tyr Arg Thr Glu Pro Ala Leu Gly Asp Ala Ile 50 55 60 Ala Glu Ala Leu Gln Leu Gly Leu Ile Asn Ser Arg Asp Asp Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Thr Asp Asn His Pro Asp Leu Val Leu 85 90 95 Pro Ala Leu His Thr Thr Leu Arg Asn Leu Lys Leu Asp Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Val Ser Met Thr Pro Gly Arg Ile Arg 115 120 125 Phe Pro Gly Pro Asp Glu Lys Ile Leu Pro Met Asp Tyr Lys Ser Val 130 135 140 Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Thr Cys Lys Lys Leu Glu Leu Leu Leu Ala Ser 165 170 175 Ala Thr Ile Pro Pro Ala Val Asn Gln Val Glu Val Asn Pro Met Trp 180 185 190 Gln Gln Glu Lys Leu Ile Glu Phe Cys Arg Thr Lys Gly Ile Val Val 195 200 205 Thr Ala Tyr Ala Pro Leu Gly Thr Val Gly Thr Phe Ile Gly Asn Asn 210 215 220 Asp Ile Met Asn Cys Glu Leu Leu Lys Glu Ile Ala Gln Ala Lys Gly 225 230 235 240 Lys Thr Asn Gly Gln Ile Cys Leu Arg Trp Val His Glu Gln Gly Val 245 250 255 Gly Leu Leu Val Lys Ser Phe Thr Glu Val Arg Met Lys Gln Asn Leu 260 265 270 Glu Ile Phe Asp Trp Glu Leu Ser Glu Glu Glu Ser Met Lys Ile Lys 275 280 285 Gln Leu Pro Gln Arg Lys Ser His Gln Gly Lys Gly Phe Val Thr Ala 290 295 300 Asp Gly Pro Phe Lys Ser Leu Thr Glu Leu Trp Asp Gly Glu Met 305 310 315 <210> 89 <211> 317 <212> PRT <213> Nigella sativa <400> 89 Met Val Tyr Glu Ile Met Leu Asn Ser Gly Tyr Arg Ile Pro Leu Leu 1 5 10 15 Gly Met Gly Thr Ala Ala Tyr Pro Val Pro Pro Pro Glu Leu Val Glu 20 25 30 Ser Ser Val Leu Ala Ala Ile Glu Leu Gly Tyr Arg His Phe Asp Thr 35 40 45 Ala His Ile Tyr Gly Thr Glu Ala Pro Leu Gly Arg Ala Ile Ser Glu 50 55 60 Ala Leu Arg Lys Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe Ile Thr 65 70 75 80 Thr Lys Leu Trp Pro Gly His Ala Arg Pro Asp Leu Val Leu Gln Ala 85 90 95 Leu His Gln Ser Leu Glu Ala Leu Arg Ile Asp Tyr Val Asp Leu Phe 100 105 110 Leu Ile His Phe Pro Ala Arg Tyr Asn Thr Thr Glu Ile Arg Met Asp 115 120 125 Ile Lys Lys Glu Asp Ile Leu Pro Leu Asp Ile Lys Gly Thr Trp Gln 130 135 140 Ala Met Glu Glu Cys Cys Lys Leu Gly Leu Ala Lys Ser Ile Gly Val 145 150 155 160 Ser Asn Phe Gly Cys Lys Lys Leu Ser Gln Leu Leu Asp Ser Ala Thr 165 170 175 Ile Pro Pro Ala Val Asn Gln Val Glu Met His Pro Leu Trp Gln Gln 180 185 190 Ala Lys Leu Arg Glu Phe Cys Ala Glu Arg Gly Ile His Val Ser Ala 195 200 205 Tyr Ser Pro Leu Gly Gly Lys Gly Thr Leu Trp Gly Ser Asn Ala Val 210 215 220 Leu Asp Ser Glu Gln Ile Lys Gln Ile Ala Leu Ala Lys Gly Lys Ser 225 230 235 240 Val Ala Gln Ile Cys Leu Arg Trp Gly Phe Gln Gln Gly Val Ser Ile 245 250 255 Ile Ala Lys Ser Phe Asn Arg Glu Arg Leu Lys Glu Asn Met Glu Ile 260 265 270 Phe Asp Trp Glu Leu Asn Lys Glu Glu Leu Tyr Lys Leu Ser Ile Leu 275 280 285 Pro Gln Asn Arg Ile Ser Thr Leu Glu Tyr Leu Val Ser Pro Asp Ala 290 295 300 Val Phe Lys Ser Ile Asn Asp Leu Trp Asp Glu Glu Val 305 310 315 <210> 90 <211> 318 <212> PRT <213> Nigella sativa <400> 90 Met Asp Asn Ser Val Val Val Leu Asn Ser Gly His Arg Met Pro Leu 1 5 10 15 Leu Gly Met Gly Thr Ala Ala Thr Pro Leu Pro Pro Asn Glu Val Val 20 25 30 Gln Ser Ser Val Leu Ala Ala Ile Glu Leu Gly Tyr Arg His Phe Asp 35 40 45 Thr Ala Ser Val Tyr Gly Ser Glu Val Ile Leu Gly Arg Ala Ile Ser 50 55 60 Glu Ala Leu Arg Arg Gly Leu Val Ala Ser Arg Asp Glu Leu Phe Ile 65 70 75 80 Thr Thr Lys Leu Trp Cys Gly Asn Gly His Pro Asp Leu Val Leu Gly 85 90 95 Ala Leu Gln Glu Ser Leu Arg Ile Met Glu Leu Asp Tyr Val Asp Leu 100 105 110 Tyr Leu Ile His Phe Pro Val Arg Phe Asn Ile Thr Glu Lys Leu Leu 115 120 125 Asn Met Lys Asn Val Pro Leu Leu Pro Phe Asp Met Glu Gly Thr Trp 130 135 140 Gln Ala Met Glu Glu Cys Cys Arg Leu Gly Leu Ala Lys Ser Ile Gly 145 150 155 160 Val Ser Asn Phe Ser Cys Lys Arg Leu Ser Glu Leu Leu Leu Ser Ala 165 170 175 Ser Ile Pro Pro Ala Val Asn Gln Val Glu Met His Pro Leu Trp Gln 180 185 190 Gln Arg Asn Met Arg Glu Phe Cys Arg Lys Glu Gly Ile His Val Ser 195 200 205 Ala Tyr Ser Pro Leu Gly Gly Lys Gly Ala Ala Trp Gly Ser Asn Ala 210 215 220 Val Leu Asp Ser Glu Asp Ile Gln Gln Ile Ala Gln His Lys Glu Lys 225 230 235 240 Ser Val Ala Gln Val Cys Leu Arg Trp Ala Phe Glu Gln Gly Val Ser 245 250 255 Ile Ile Val Lys Ser Phe Asn Lys Glu Arg Leu Lys Glu Asn Thr Gln 260 265 270 Ile Phe Asp Trp Glu Leu Asn Lys Gln Glu Leu Gln Arg Ile Asn Thr 275 280 285 Leu Pro Gln Asn Lys Ile Phe Lys Ala Ser Asn Leu Ile Ser Ala Asp 290 295 300 Gly Pro Tyr Lys Thr Phe Glu Glu Leu Trp Asp Gly Glu Val 305 310 315 <210> 91 <211> 321 <212> PRT <213> Papaver bracteatum <400> 91 Met Glu Ser Asn Gly Val Pro Met Ile Thr Leu Ser Ser Gly Ile Arg 1 5 10 15 Met Pro Ala Leu Gly Met Gly Thr Val Glu Thr Met Glu Lys Gly Thr 20 25 30 Glu Arg Glu Lys Leu Ala Phe Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 His Phe Asp Ala Ala Ala Ala Tyr Gln Thr Glu Glu Cys Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Glu Glu 65 70 75 80 Leu Phe Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Ala Asp Leu 85 90 95 Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Leu Asp Leu Tyr Leu Ile His Phe Pro Val Ser Leu Lys Pro Gly Lys 115 120 125 Ile Val Ser Asp Ile Pro Lys Asp Gln Met Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Thr Leu Gly Phe Thr Arg 145 150 155 160 Ala Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu Met 165 170 175 Ala Thr Ala Asn Ser Pro Pro Val Val Asn Glu Val Glu Met Ser Pro 180 185 190 Ile Phe Gln Gln Lys Lys Leu Arg Ala Tyr Cys Lys Ala Asn Asn Ile 195 200 205 Met Ile Thr Ala Tyr Ser Val Leu Gly Ala Arg Gly Ala Ala Trp Gly 210 215 220 Ser Asn Ala Val Met Asp Ser Lys Val Leu His Glu Ile Ala Val Ser 225 230 235 240 Arg Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Gln Gln 245 250 255 Gly Ala Cys Leu Val Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu 260 265 270 Asn Leu Lys Ile Phe Asp Trp Glu Leu Ser Ala Glu Asp Met Glu Lys 275 280 285 Ile Ser Glu Ile Pro Gln Ser Arg Thr Ser Ser Ala Asp Phe Leu Leu 290 295 300 Ser Pro Thr Gly Pro Phe Lys Thr Glu Glu Glu Phe Trp Asp Glu Lys 305 310 315 320 Asp <210> 92 <211> 321 <212> PRT <213> Papaver bracteatum <400> 92 Met Glu Ile Asn Gly Val Pro Val Ile Ser Leu Ser Ser Gly Val Arg 1 5 10 15 Met Pro Ala Leu Gly Met Gly Thr Ala Glu Thr Met Glu Lys Gly Thr 20 25 30 Asp Arg Glu Arg Ser Ala Phe Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 His Phe Asp Thr Ala Ala Ala Tyr Gln Thr Glu Glu Cys Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Ala Asp Leu 85 90 95 Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Leu Asp Leu Tyr Leu Ile His Ala Pro Val Ser Leu Lys Pro Gly Lys 115 120 125 Ile Leu Asn Glu Ile Pro Lys Asp Gln Met Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Thr Leu Gly Phe Thr Arg 145 150 155 160 Ala Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu Met 165 170 175 Ala Thr Ala Asn Ser Pro Pro Val Val Asn Glu Val Glu Met Ser Pro 180 185 190 Thr Leu His Gln Lys Asn Leu Arg Glu Tyr Cys Lys Ala Asn Asn Ile 195 200 205 Met Ile Ile Ala Tyr Ser Val Leu Gly Ala Arg Gly Thr Gly Trp Gly 210 215 220 Ser Asn Ala Val Met Asp Ser Lys Val Leu His Gln Ile Ala Val Ala 225 230 235 240 Arg Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Gln Gln 245 250 255 Gly Ala Cys Leu Val Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu 260 265 270 Asn Leu Lys Ile Phe Asp Trp Glu Leu Thr Glu Glu Asp Met Asp Lys 275 280 285 Ile Ser Glu Ile Pro Gln Ser Arg Thr Leu Ser Ala Asp Phe Leu Leu 290 295 300 Ser Pro Thr Gly Pro Phe Lys Thr Glu Glu Glu Phe Trp Asp Glu Met 305 310 315 320 Val <210> 93 <211> 323 <212> PRT <213> Papaver bracteatum <400> 93 Met Arg Asn Thr Gly Val Pro Val Ile Thr Leu Ser Ser Gly Lys Gly 1 5 10 15 Ile Pro Val Leu Gly Met Gly Thr Phe Glu Thr Val Gly Lys Gly Gly 20 25 30 Glu Arg Glu Arg Leu Ala Phe Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 His Phe Asp Thr Ala Ala Cys Tyr Gln Thr Glu Glu Cys Leu Gly Glu 50 55 60 Ala Ile Glu Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Thr Ser Lys Leu Trp Cys Thr Asp Ala His Pro Asp Arg 85 90 95 Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Leu Asp Leu Tyr Leu Ile His Phe Pro Val Ser Leu Lys Pro Gly Asn 115 120 125 Glu Val Thr Met Asp Ala Ala Gly Gly Glu Ile Phe Leu Met Asp Tyr 130 135 140 Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Asn Leu Gly Phe Thr 145 150 155 160 Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu 165 170 175 Leu Ala Thr Ala Asn Ile Pro Pro Val Val Asn Gln Val Glu Met Ser 180 185 190 Pro Val Phe His Gln Lys Asn Leu Arg Glu Tyr Cys Lys Ala Asn Asn 195 200 205 Ile Leu Val Ala Ala Tyr Ser Ile Leu Gly Gly Lys Gly Thr Ala Trp 210 215 220 Gly Ser Asn Ser Val Leu Gly Ser Glu Gly Leu Asn Gln Ile Ala Ile 225 230 235 240 Ala Arg Gly Lys Ser Ile Ala Gln Val Ser Met Arg Trp Val Tyr Glu 245 250 255 Gln Gly Ala Ile Leu Val Val Lys Ser Phe Ser Glu Lys Asn Met Arg 260 265 270 Glu Asn Leu Asn Ile Phe Asp Trp Glu Leu Thr Lys Glu Asp Leu Glu 275 280 285 Arg Ile Gly Glu Ile Pro Gln Arg Arg Leu Ile Ile Gln Glu Phe Met 290 295 300 Val Ser Ser Asn Gly Pro Phe Lys Ser Leu Glu Glu Phe Trp Asp Glu 305 310 315 320 Lys Ala Asp <210> 94 <211> 321 <212> PRT <213> Papaver bracteatum <400> 94 Met Glu Asn Val Ile Pro Ala Val Thr Leu Ser Ser Gly Ser Val Met 1 5 10 15 Pro Ile Leu Gly Met Gly Thr Ala Ala Tyr Pro Leu Val Glu Pro Glu 20 25 30 Glu Ala Lys Leu Ala Phe Leu Asn Ala Ile Lys Ile Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ala Ser Tyr His Cys Glu Gly Phe Leu Gly Glu Ala 50 55 60 Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Pro Cys Asp Ala His Pro Asp Leu Val 85 90 95 Ile Pro Ala Ile Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Ile Ser Thr Lys Pro Ala Ala Gly 115 120 125 Leu Val Phe Pro Pro Pro Lys Asp Ala Leu Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu 165 170 175 Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro 180 185 190 Val Trp Gln Asn Leu Lys Leu Arg Asp Phe Cys Lys Ala Asn Asn Ile 195 200 205 Val Leu Thr Ala Tyr Ser Pro Leu Gly Ala Arg Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Tyr Glu Glu Arg Val Leu His Glu Ile Ala Glu Ala 225 230 235 240 Lys Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln 245 250 255 Gly Val Ser Leu Ile Val Lys Ser Phe Asn Glu Leu Arg Met Lys Glu 260 265 270 Asn Met Met Ile Phe Asp Trp Glu Leu Thr Glu Asp Glu Leu Gln Lys 275 280 285 Ile Gly Lys Ile Pro Gln Arg Arg Gly Leu Pro Gly Asp Phe Phe Val 290 295 300 Ser Glu Ala Ala Pro Phe Lys Thr Val Glu Glu Phe Trp Asp Gly Glu 305 310 315 320 Ile <210> 95 <211> 322 <212> PRT <213> Papaver bracteatum <400> 95 Met Glu Val Val Ile Pro Lys Val Ala Leu Ser Ser Gly Arg Val Met 1 5 10 15 Pro Val Leu Gly Met Gly Thr Ser Ser Phe Pro Pro Val Gly Pro Glu 20 25 30 Asp Gly Lys Ala Ala Ile Leu Asn Ala Ile Lys Ile Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ser Val Tyr Lys Ser Glu Asp Phe Leu Gly Glu Ala 50 55 60 Ile Ala Glu Ala Leu Leu Leu Gly Leu Ile Gln Ser Arg Asp Gln Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Tyr Cys Asn Asp Ala His Pro Asp Leu Val 85 90 95 Val Pro Ala Leu Gln Asn Ser Leu Arg Asn Leu Arg Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Val Ser Ser Lys Pro Val Lys Tyr 115 120 125 Glu Tyr His Leu Lys Lys Glu His Leu Leu Pro Met Asp Tyr Glu Ser 130 135 140 Val Trp Ala Ala Met Glu Lys Cys Gln Lys Leu Gly Leu Thr Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu Asp 165 170 175 Thr Ala Asn Ile Ser Pro Ala Val Asn Gln Val Glu Met Asn Pro Val 180 185 190 Trp Gln Asn Leu Lys Leu Arg Asp Phe Cys Lys Ala Lys Gly Ile Val 195 200 205 Val Thr Ala Tyr Ser Pro Leu Gly Ala Ser Gly Thr Pro Trp Gly Ser 210 215 220 Asn Ala Val Lys Glu Ala Gln Glu Leu His Glu Ile Ala Lys Ala Arg 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln Gly 245 250 255 Val Ser Leu Leu Val Lys Ser Phe Lys Glu Glu Arg Met Lys Glu Asn 260 265 270 Leu Met Ile Phe Asp Trp Glu Leu Thr Lys Asp Asp Leu Phe Lys Ile 275 280 285 Ser Lys Ile Thr Gln Arg Arg Gly Leu Pro Gly Tyr Arg Phe Ile Ser 290 295 300 Lys Leu Glu Gly Ser Pro Phe Lys Thr Val Glu Glu Phe Trp Asp Gly 305 310 315 320 Glu Val <210> 96 <211> 321 <212> PRT <213> Papaver bracteatum <400> 96 Met Glu Ser Ser Gly Val Pro Val Ile Thr Leu Arg Ser Gly Lys Val 1 5 10 15 Met Pro Val Leu Gly Met Gly Thr Phe Glu Lys Ala Gly Lys Gly Ser 20 25 30 Glu Arg Glu Arg Leu Ala Ile Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 Tyr Phe Asp Thr Ala Ala Ala Tyr Glu Thr Glu Glu Val Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Ser Ser Met Leu Trp Cys Thr Asp Ala His Pro Asp Arg 85 90 95 Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Val Asp Leu Tyr Met Leu Pro Phe Pro Ala Ser Leu Lys Pro Gly Lys 115 120 125 Ile Thr Met Asp Ile Pro Glu Glu Asp Ile Cys Pro Met Asp Tyr Arg 130 135 140 Ser Val Trp Ser Ala Met Glu Glu Cys Gln Asn Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Glu Leu Met 165 170 175 Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Ser Pro 180 185 190 Ala Phe Gln Gln Lys Lys Leu Arg Glu Tyr Cys Asn Ala Asn Asn Ile 195 200 205 Leu Val Ser Ala Val Ser Ile Leu Gly Ser Asn Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Leu Gly Ser Glu Val Leu Lys Lys Ile Ala Met Ala 225 230 235 240 Lys Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Glu Gln 245 250 255 Gly Ala Ser Leu Val Val Lys Ser Phe Ser Glu Glu Arg Leu Arg Glu 260 265 270 Asn Leu Asn Ile Phe Asp Trp Gln Leu Thr Lys Glu Asp Asn Glu Lys 275 280 285 Ile Gly Glu Ile Pro Gln Cys Arg Ile Leu Ser Ala Tyr Phe Leu Val 290 295 300 Ser Pro Lys Gly Pro Phe Lys Ser Gln Glu Glu Leu Trp Asp Asp Lys 305 310 315 320 Ala <210> 97 <211> 323 <212> PRT <213> Papaver bracteatum <400> 97 Met Gly Ser Ser Cys Ile Pro Val Leu Thr Leu Asn Ser Gly Asn Lys 1 5 10 15 Met Pro Val Leu Gly Met Gly Thr Phe Glu Thr Phe Ala Lys Gly Gly 20 25 30 Glu Arg Glu Arg Leu Ala Tyr Leu Lys Ala Ile Lys Val Gly Tyr Arg 35 40 45 Tyr Phe Asp Thr Gly Ala Ala Tyr Gly Thr Glu Glu Val Leu Gly Gln 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Ser Thr Met Ile Trp Ala Thr Asp Ala His Pro Asp Gly 85 90 95 Val Leu Pro Ala Val Gln Arg Ser Leu Arg Asn Leu Lys Leu Asp Tyr 100 105 110 Val Asp Leu Tyr Leu Ile Pro Phe Pro Ala Ser Leu Asn Pro Glu Gly 115 120 125 Glu Ile Pro Asn Tyr Ile Pro Glu Asn Glu Thr Phe Leu Lys Met Asp 130 135 140 Tyr Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Thr Leu Gly Phe 145 150 155 160 Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu 165 170 175 Val Met Glu Thr Ala Asn Ile Pro Pro Ala Val Asn Met Val Glu Met 180 185 190 Asn Pro Thr Phe Gln Gln Lys Tyr Leu Arg Glu Tyr Cys Lys Ala Asn 195 200 205 Asn Ile Leu Val Asn Ala Tyr Ser Val Leu Gly Ser Thr Gly Thr Ser 210 215 220 Trp Gly Ser Asn Ala Val Met Gly Ser Glu Val Leu Lys Gln Ile Ala 225 230 235 240 Thr Asp Ile Gly Lys Ser Ile Ala Gln Val Ser Met Arg Trp Val Tyr 245 250 255 Glu Gln Gly Ala Gly Phe Val Val Lys Ser Phe Ser Glu Glu Arg Met 260 265 270 Arg Glu Asn Leu Asn Ile Phe Asp Trp Glu Leu Thr Lys Glu Asp Leu 275 280 285 Glu Lys Ile Ser Gln Ile Pro Gln Cys Arg Val Leu Pro Met Asp Phe 290 295 300 Leu Val Ser Ser Asp Gly Ala Phe Lys Ser Leu Glu Asp Leu Trp Asp 305 310 315 320 Gly Glu Ala <210> 98 <211> 303 <212> PRT <213> Papaver bracteatum <400> 98 Met Pro Ile Leu Gly Phe Gly Thr Ala Glu Asn Leu Phe Glu Gly Gly 1 5 10 15 Asp Lys Val Lys Leu Ala Ile Leu Lys Ala Ile Glu Val Gly Tyr Arg 20 25 30 Tyr Ile Asp Thr Ala Ala Val Tyr Arg Thr Glu Glu Ser Val Gly Glu 35 40 45 Ala Val Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 50 55 60 Leu Phe Ile Thr Ser Lys Leu Trp Gly Val Asp Ala His Pro Asp Leu 65 70 75 80 Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Lys Leu Lys Leu Glu Tyr 85 90 95 Leu Asp Leu Tyr Leu Ile His Tyr Pro Val Ser Leu Lys Pro Gly Glu 100 105 110 Met Val Asp Asp Ile Pro Lys Asp Glu Ile Phe Pro Leu Asp Tyr Lys 115 120 125 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Tyr Thr Lys 130 135 140 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Gln Leu Met 145 150 155 160 Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro 165 170 175 Thr Trp Gln Gln Lys Asn Leu Arg Glu Tyr Cys Lys Ala Asn Asn Ile 180 185 190 Phe Ile Thr Ala Tyr Ser Thr Leu Gly Ala Lys Asp Leu Leu Trp Gly 195 200 205 Ser Asn Ala Val Leu Gly Ser Lys Val Leu Asn Gln Ile Ala Val Ala 210 215 220 Arg Gly Lys Ser Val Ala Gln Val Ser Leu Arg Trp Val Tyr Glu Gln 225 230 235 240 Gly Val Ser Leu Val Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu 245 250 255 Asn Leu Lys Ile Phe Asp Trp Glu Leu Thr Thr Glu Asp Leu Lys Met 260 265 270 Ile Ser Glu Ile Pro Gln Arg Arg Val Ala Thr Ala Asp Phe Phe Val 275 280 285 Ser Asp Ile Gly Pro Phe Lys Ser Leu Glu Glu Leu Trp Asp Glu 290 295 300 <210> 99 <211> 306 <212> PRT <213> Papaver bracteatum <400> 99 Met Pro Val Leu Gly Leu Gly Thr Ala Glu Asn Leu Thr Lys Gly Ser 1 5 10 15 Glu Arg Glu Met Leu Ala Ile Leu Lys Ala Ile Glu Val Gly Tyr Arg 20 25 30 His Phe Asp Thr Ala Phe Ile Tyr Gln Thr Gln Glu Cys Val Gly Glu 35 40 45 Ala Ile Ala Glu Ala Leu Gln Leu Gly Ile Ile Lys Ser Arg Asp Glu 50 55 60 Leu Phe Ile Thr Ser Lys Leu Trp Gly Ser Asp Ala His Pro Asp Cys 65 70 75 80 Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Gln Tyr 85 90 95 Leu Asp Leu Tyr Leu Ile His Tyr Pro Val Ser Leu Lys Pro Gly Thr 100 105 110 Thr Leu Lys Asp Leu Gly Asn Lys Asp Asn Phe Leu Pro Met Asp Tyr 115 120 125 Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr 130 135 140 Lys Ser Ile Gly Val Ser Asn Phe Ser Ser Lys Lys Ile Gln Glu Leu 145 150 155 160 Met Ser Thr Asp Ser Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn 165 170 175 Pro Thr Trp Gln Gln Lys Lys Leu Arg Glu Tyr Cys Gln Ala Asn Asn 180 185 190 Ile Leu Val Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr Thr Trp 195 200 205 Gly Ser Asn Ala Val Met Gly Ser Glu Val Leu Asn Gln Ile Ala Leu 210 215 220 Ala Arg Gly Lys Ser Val Ala Gln Ile Ser Leu Arg Trp Val Tyr Glu 225 230 235 240 Gln Gly Val Ser Leu Val Val Lys Ser Phe Asn Glu Glu Arg Met Arg 245 250 255 Glu Asn Leu Lys Ile Phe Asp Trp Glu Leu Thr Ala Glu Asp Leu Lys 260 265 270 Lys Ile Asp Glu Leu Pro Gln Ser Arg Val Ala Thr Ala Glu Phe Val 275 280 285 Val Ser Glu Asn Gly Pro Phe Lys Ser Leu Glu Glu Phe Trp Asp Asp 290 295 300 Glu Ser 305 <210> 100 <211> 321 <212> PRT <213> Papaver bracteatum <400> 100 Met Glu Asn Ala Ile Pro Ala Val Thr Leu Asn Ser Gly Ser Val Met 1 5 10 15 Pro Val Leu Gly Met Gly Thr Ala Ala Tyr Pro Phe Val Glu Ser Glu 20 25 30 Glu Ala Lys Leu Ala Ile Leu Asn Ala Ile Lys Thr Gly Tyr Arg His 35 40 45 Leu Asp Thr Ala Ala Leu Tyr Gln Ser Glu Glu Ser Leu Gly Glu Ala 50 55 60 Val Ser Glu Ala Ile Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Pro Cys Asp Ala His Pro Asp Leu Val 85 90 95 Ile Pro Ala Ile Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Ile Ser Thr Lys Pro Ala Ala Gly 115 120 125 Leu Val Phe Pro Pro Pro Lys Asp Ala Leu Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu 165 170 175 Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro 180 185 190 Val Trp Gln Asn Leu Gln Leu Arg Asp Phe Cys Lys Asp Lys Ser Ile 195 200 205 Ile Leu Thr Ala Tyr Ser Pro Leu Glu Gly Lys Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Tyr Gly Ala Gln Val Leu His Glu Ile Ala Glu Ala 225 230 235 240 Lys Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln 245 250 255 Gly Val Ser Leu Leu Val Lys Ser Phe Asn Asp Gln Arg Met Lys Glu 260 265 270 Asn Met Met Ile Phe Asp Trp Glu Leu Thr Glu Asp Glu Leu Glu Lys 275 280 285 Ile Ser Arg Ile Pro Gln Arg Arg Gly Leu Pro Gly Asp Phe Phe Val 290 295 300 Ser Glu Ala Ala Pro Phe Lys Thr Val Glu Glu Phe Trp Asp Gly Glu 305 310 315 320 Ile <210> 101 <211> 321 <212> PRT <213> Papaver bracteatum <400> 101 Met Glu Asn Ala Ile Pro Ala Val Thr Leu Asn Ser Gly Ser Val Met 1 5 10 15 Pro Val Leu Gly Met Gly Thr Ala Ala Tyr Pro Phe Val Glu Ser Glu 20 25 30 Glu Ala Lys Leu Ala Ile Leu Asn Ala Ile Lys Thr Gly Tyr Arg His 35 40 45 Leu Asp Thr Ala Ala Leu Tyr Gln Ser Glu Glu Ser Leu Gly Glu Ala 50 55 60 Val Ala Glu Ala Ile Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Pro Cys Asp Ala His Pro Asp Arg Val 85 90 95 Val Pro Thr Leu Gln Asn Ser Leu Arg Lys Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser Asn Pro Val Ala Gly 115 120 125 His Val Leu Ser Leu Pro Lys Asp Ser Leu Val Thr Met Asp Tyr Glu 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu 165 170 175 Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro 180 185 190 Val Trp Gln Asn Leu Lys Leu Arg Asp Phe Cys Lys Ala Asn Asn Ile 195 200 205 Val Leu Thr Ala Tyr Ser Pro Leu Gly Ala Arg Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Tyr Glu Glu Arg Val Leu His Glu Ile Ala Glu Ala 225 230 235 240 Lys Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln 245 250 255 Gly Val Ser Leu Ile Val Lys Ser Phe Asn Glu Leu Arg Met Lys Glu 260 265 270 Asn Met Met Ile Phe Asp Trp Glu Leu Thr Glu Asp Glu Leu Gln Lys 275 280 285 Ile Gly Lys Ile Pro Gln Arg Arg Gly Leu Pro Gly Asp Phe Phe Val 290 295 300 Ser Glu Ala Ala Pro Phe Lys Thr Val Glu Glu Phe Trp Asp Gly Glu 305 310 315 320 Ile <210> 102 <211> 323 <212> PRT <213> Papaver bracteatum <400> 102 Met Glu Asn Val Ile Pro Ala Val Thr Leu Ser Ser Gly Ser Val Met 1 5 10 15 Pro Ile Leu Ala Met Gly Thr Ala Ala Tyr Pro Leu Val Glu Pro Glu 20 25 30 Glu Ala Lys Leu Ala Phe Leu Asn Ala Ile Lys Ile Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ala Ser Tyr His Cys Glu Gly Phe Leu Gly Glu Ala 50 55 60 Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Pro Cys Asp Ala His Pro Asp Leu Val 85 90 95 Ile Pro Ala Ile Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Phe Pro Ile Ser Thr Lys Pro Ala Ala Gly 115 120 125 Leu Val Phe Pro Pro Pro Lys Asp Ala Leu Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Thr Ile Leu 165 170 175 Asp Ile Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro 180 185 190 Val Trp Gln Asn Leu Gln Leu Arg Asp Phe Cys Lys Asp Lys Ser Ile 195 200 205 Ile Leu Thr Ala Tyr Ser Pro Leu Glu Gly Lys Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Tyr Gly Ala Gln Val Leu His Glu Ile Ala Glu Ala 225 230 235 240 Lys Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln 245 250 255 Gly Val Ser Leu Leu Val Lys Ser Phe Asn Asp Gln Arg Met Lys Glu 260 265 270 Asn Met Met Ile Phe Asp Trp Glu Leu Thr Glu Asp Glu Leu Glu Lys 275 280 285 Ile Gly Lys Ile Pro Gln Ser Arg Gly Leu Pro Gly Asp Val Phe Val 290 295 300 Ser Glu Leu Glu Ala Ala Pro Phe Lys Thr Val Glu Glu Phe Trp Asp 305 310 315 320 Gly Glu Val <210> 103 <211> 319 <212> PRT <213> Sanguinaria canadensis <400> 103 Met Glu Asn Val Ser Val Val Thr Leu Asn Ser Gly Arg Glu Met Pro 1 5 10 15 Ile Leu Gly Met Gly Thr Ala Ala Tyr Pro Ser Val Gly Ser Glu Glu 20 25 30 Ala Lys Leu Ala Ile Leu Leu Ala Ile Lys Val Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ala Ala Tyr Gln Ile Glu Ser Ser Leu Gly Glu Ala Val 50 55 60 Ala Glu Ala Leu Gln Leu Gly Leu Leu Lys Ser Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Ser Asp Ser His Pro Glu Arg Val Leu 85 90 95 Pro Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Val Ser Leu Lys Pro Gly Asn Phe Asp 115 120 125 Leu Thr Ile Pro Lys Glu Asp Leu Leu Pro Met Asp Tyr Lys Ser Val 130 135 140 Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Asp Leu Leu Ala Thr 165 170 175 Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro Ile Trp 180 185 190 Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys Ala Asn Gly Ile Leu Ile 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Ala Asn Gly Thr Pro Trp Gly Ser Ser 210 215 220 Gly Val Ala Asn Thr Glu Val Leu His Gln Ile Ala Lys Ala Arg Gly 225 230 235 240 Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln Gly Val 245 250 255 Ser Leu Leu Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn Leu 260 265 270 Lys Ile Phe Asp Trp Glu Leu Thr Ala Glu Asp Leu Lys Gln Ile Ser 275 280 285 Glu Ile Pro Gln His Arg Gly Leu Pro Ala Asp Ile Phe Val Ser Val 290 295 300 Asn Gly Pro Phe Lys Thr Val Glu Glu Phe Trp Asp Gly Glu Val 305 310 315 <210> 104 <211> 321 <212> PRT <213> Sanguinaria canadensis <400> 104 Met Glu Ile Ser Val Pro Ile Thr Thr Leu Ser Ser Gly Arg Glu Met 1 5 10 15 Pro Ile Leu Gly Met Gly Thr Ala Glu Asn Leu Phe Asn Gly Ser Glu 20 25 30 Lys Val Lys Leu Ala Ile Leu Asp Ala Ile Lys Val Gly Tyr Arg His 35 40 45 Phe Asp Thr Ala Ala Val Tyr Gln Thr Glu Ser Ser Leu Gly Glu Ala 50 55 60 Val Ala Glu Ala Leu Gln His Gly Leu Leu Lys Ser Arg Asp Glu Leu 65 70 75 80 Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp Ser His Pro Asp Arg Val 85 90 95 Leu Pro Ala Leu Gln Thr Ser Leu Arg Lys Leu Lys Leu Glu Tyr Leu 100 105 110 Asp Leu Tyr Leu Ile His Trp Pro Leu Ser Ser Lys Pro Gly Ser His 115 120 125 Asp Tyr Pro Ile Pro Lys Glu Asp Leu Leu Pro Leu Asp Tyr Lys Ser 130 135 140 Val Trp Ala Ala Met Glu Glu Cys Gln Lys Leu Gly Leu Thr Lys Ser 145 150 155 160 Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Asp Leu Leu Asp 165 170 175 Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro Leu 180 185 190 Trp Gln Gln Lys Lys Leu Leu Glu Phe Cys Lys Gly Asn Gly Ile Ile 195 200 205 Ile Thr Ala Phe Ser Pro Leu Gly Ala Lys Gly Thr Ser Trp Gly Ala 210 215 220 Thr Asn Gly Val Met Asp Ser Glu Val Leu His Gln Ile Ala Gln Ala 225 230 235 240 Arg Gly Lys Ser Ile Ala Gln Val Ser Leu Arg Trp Leu Tyr Glu Gln 245 250 255 Gly Val Ser Leu Val Val Lys Ser Phe Asn Val Glu Arg Met Lys Glu 260 265 270 Asn Leu Lys Ile Phe Asp Trp Glu Leu Ser Ala Glu Asp Leu Lys Lys 275 280 285 Ile Asn Glu Ile Pro Gln Arg Arg Gly Leu Pro Ser Gly Ser Phe Ile 290 295 300 Ser Ala Asn Gly Pro Phe Lys Ser Glu Glu Glu Leu Trp Asp Gly Glu 305 310 315 320 Val <210> 105 <211> 318 <212> PRT <213> Sanguinaria canadensis <400> 105 Met Ala Met Glu Thr Val Pro Lys Leu Pro Leu Ser Ser Gly Asp Asn 1 5 10 15 Ser Ile Pro Val Ile Gly Phe Gly Thr Ala Ala Phe Pro Leu Pro Asp 20 25 30 Asp Glu Thr Leu Lys Ser Ala Phe Leu Asn Gly Ile Glu Ala Gly Tyr 35 40 45 Arg His Phe Asp Thr Ala Ala Ala Tyr Gly Ser Glu Lys Ala Leu Gly 50 55 60 Glu Ala Ile Thr Glu Ala Leu Arg Leu Gly Leu Leu Lys Ser Arg Glu 65 70 75 80 Glu Val Phe Ile Thr Thr Lys Leu Trp Cys Ser Ser Cys Glu Arg Ser 85 90 95 Leu Val Val Pro Ser Leu Lys Asn Ser Leu Arg Asn Leu Gln Met Glu 100 105 110 Tyr Val Asp Leu Phe Leu Ile His Trp Pro Val Arg Leu Ser Ile Asp 115 120 125 Ala Gln Arg Pro Pro Ile Pro Arg Glu Gln Ile Leu Thr Phe Asp Thr 130 135 140 Lys Ser Val Trp Glu Gly Met Glu Glu Cys His Glu Leu Gly Leu Ala 145 150 155 160 Lys Asn Ile Gly Leu Ser Asn Phe Tyr Pro Lys Lys Ile Asp Glu Leu 165 170 175 Leu Ala Thr Ala Lys Ile Pro Pro Ala Val Leu Gln Val Glu Leu Ser 180 185 190 Pro Thr Trp Gln Gln Lys Asn Leu Ile Glu Tyr Cys Arg Glu Lys Gly 195 200 205 Ile Leu Val Thr Ala Tyr Ser Ala Leu Gly Ala Asn Gly Thr His Trp 210 215 220 Gly Asp Asn Arg Val Val Glu Ser Asp Val Leu Gly Asp Ile Ala Lys 225 230 235 240 Ala Arg Gly Lys Thr Thr Ala Gln Ile Ala Leu Arg Trp Val Tyr Glu 245 250 255 Gln Gly Val Cys Met Val Val Lys Ser Phe Asn Lys Glu Arg Met Lys 260 265 270 Leu Asn Leu Gln Ile Phe Asp Phe Glu Leu Thr Glu Glu Glu Ser Asn 275 280 285 Lys Ile Ser Gln Leu Pro Gln Ile Lys Gly Val Lys Leu Ser Pro Met 290 295 300 Phe Gly Asn His Asp Val Leu Lys Glu Leu Glu Asp Gln Leu 305 310 315 <210> 106 <211> 328 <212> PRT <213> Sanguinaria canadensis <400> 106 Met Gly Glu Glu Met Lys Cys Gly Arg Val Val Pro Val Ala Thr Leu 1 5 10 15 Asn Ser Gly Arg Thr Met Pro Leu Leu Gly Met Gly Thr Ala Ala Tyr 20 25 30 Pro Phe Val Gly Ser Glu Lys Val Lys Ser Ala Ile Leu His Ala Ile 35 40 45 Lys Leu Gly Tyr Arg His Phe Asp Thr Ala Ala Leu Tyr Gln Thr Glu 50 55 60 Glu Cys Leu Gly Glu Val Val Ala Glu Ala Leu Gln Leu Gly Leu Leu 65 70 75 80 Lys Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp 85 90 95 Ser His Pro Asp Arg Val Leu Pro Ala Leu Gln Thr Ser Leu Arg Lys 100 105 110 Leu Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Leu Ser 115 120 125 Leu Lys Pro Gly Asn Tyr Gly Phe Pro Ile Pro Lys Glu Asp Met Leu 130 135 140 Pro Met Asp Tyr Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys 145 150 155 160 Leu Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys 165 170 175 Leu Gln Asp Leu Leu Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln 180 185 190 Val Glu Met Asn Pro Ile Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys 195 200 205 Glu Ala Asn Gly Ile Leu Ile Thr Ala Tyr Ser Pro Leu Gly Ala Asn 210 215 220 Gly Thr Pro Trp Gly Ser Ser Gly Val Ala Gln Thr Gln Val Leu His 225 230 235 240 Asp Ile Ala Lys Ala Arg Gly Lys Thr His Ala Gln Val Cys Val Arg 245 250 255 Trp Val Tyr Glu Gln Gly Val Ser Val Leu Val Lys Ser Tyr Asn Glu 260 265 270 Glu Arg Met Lys Glu Asn Leu Thr Val Phe Asp Trp Glu Leu Asp Val 275 280 285 Glu Asp Leu Lys Lys Ile Ser Thr Glu Ile Pro Gln Arg Arg Gly Leu 290 295 300 Pro Ser Asp Ile Phe Val Ser Val Leu Thr Gly Pro Phe Lys Ser Ala 305 310 315 320 Glu Glu Leu Trp Asp Gly Glu Leu 325 <210> 107 <211> 326 <212> PRT <213> Stylophorum diphyllum <400> 107 Met Glu Ser Asp Ser Thr Ala Val Ala Val Pro Val Thr Thr Leu Ser 1 5 10 15 Ser Gly Ile Glu Met Pro Met Leu Gly Leu Gly Thr Ala Asp Glu Lys 20 25 30 Leu Leu Pro Ser Ser Glu Thr Val Lys Leu Ala Phe Leu Thr Ala Ile 35 40 45 Lys Leu Gly Tyr Arg His Phe Asp Thr Ala Ala Val Tyr Gln Thr Glu 50 55 60 Glu Cys Leu Gly Glu Ala Val Ala Glu Ala Leu Gln Leu Gly Leu Ile 65 70 75 80 Lys Ser Arg Asp Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Ser Asp 85 90 95 Ala His Pro Asp Leu Val Ile Pro Ala Leu Gln Asn Ser Leu Arg Lys 100 105 110 Leu Lys Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Leu Ser 115 120 125 Ser Lys Pro Gly Asn Cys Val Phe Pro Ile Pro Lys Glu Asp Leu Leu 130 135 140 Pro Leu Asp Phe Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Lys 145 150 155 160 Leu Gly Leu Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys 165 170 175 Leu Gln Asp Leu Leu Ala Met Ala Ile Ile Pro Pro Ala Val Asn Gln 180 185 190 Val Glu Met Asn Pro His Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys 195 200 205 Lys Val Lys Asp Ile Val Val Thr Ala Tyr Ser Pro Leu Gly Ala Lys 210 215 220 Gly Thr Pro Trp Gly Ser Asn Ala Val Met Asp Ser Lys Val Leu His 225 230 235 240 Gln Ile Ala Lys Ala Arg Gly Lys Ser Val Ala Gln Val Ser Leu Arg 245 250 255 Trp Val Tyr Gln Gln Gly Val Val Leu Leu Val Lys Ser Phe Asn Glu 260 265 270 Glu Arg Met Lys Glu Asn Met Lys Ile Phe Asn Trp Glu Leu Asn Glu 275 280 285 Glu Asp Leu Lys Met Ile Asp Glu Ile Pro Gln Cys Arg Gly Asn Pro 290 295 300 Ser His Tyr Tyr Val Ser Glu Asn Gly Pro Phe Lys Thr Val Glu Glu 305 310 315 320 Phe Trp Asp Gly Glu Val 325 <210> 108 <211> 307 <212> PRT <213> Stylophorum diphyllum <400> 108 Met Pro Ile Val Gly Met Gly Thr Ala Glu Asn Leu Phe Glu Gly Ser 1 5 10 15 Glu Arg Val Lys Leu Ala Leu Leu Thr Ala Ile Lys Val Gly Tyr Arg 20 25 30 His Phe Asp Thr Ala Ala Val Tyr Gln Thr Glu Gly Ser Leu Gly Glu 35 40 45 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 50 55 60 Leu Phe Ile Thr Ser Lys Leu Trp Ile His Asp Cys His His Asp Leu 65 70 75 80 Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Lys Leu Lys Leu Glu Tyr 85 90 95 Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ser Ser Lys Pro Gly Glu 100 105 110 Leu Arg Ser Leu Ile Pro Lys Glu Glu Leu Leu Pro Met Asp Phe Lys 115 120 125 Ser Val Trp Val Ala Met Glu Glu Cys His Lys Ile Gly Leu Thr Lys 130 135 140 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Asp Leu Leu 145 150 155 160 Leu Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Lys Val Glu Met Asn 165 170 175 Pro Leu Trp Gln Gln Lys Lys Leu Arg Glu Phe Cys Lys Ala Asn Gly 180 185 190 Ile Val Ile Ala Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr Leu Trp 195 200 205 Gly Thr Asn Gly Val Met Asp Ser Glu Val Leu Gln Gln Ile Ala Lys 210 215 220 Ala Arg Arg Lys Ser Ile Ala Gln Val Ser Leu Arg Trp Val Tyr Glu 225 230 235 240 Gln Gly Val Val Leu Leu Val Lys Ser Phe Asn Glu Gly Arg Met Lys 245 250 255 Glu Asn Leu Lys Ile Phe Asp Trp Glu Leu Ser Arg Glu Asp Leu Lys 260 265 270 Gln Ile Asn Glu Ile Leu Pro Gln Arg Arg Gly Leu Pro Ser His Val 275 280 285 Phe Val Ser Asp Asp Gly Pro Phe Lys Ser Glu Glu Glu Leu Trp Asp 290 295 300 Gly Glu Val 305 <210> 109 <211> 318 <212> PRT <213> Stylophorum diphyllum <400> 109 Met Thr Met Glu Ala Val Pro Lys Val Thr Leu Ser Thr Ser Gly Lys 1 5 10 15 Ser Met Pro Ile Ile Gly Phe Gly Thr Ala Leu Phe Pro Thr Gly Asn 20 25 30 Asp Glu Ala Val Lys Ser Ala Val Leu Ser Gly Ile Glu Ala Gly Tyr 35 40 45 Arg His Phe Asp Thr Ala Val Ala Tyr Arg Ser Glu Lys Gly Leu Gly 50 55 60 Glu Gly Ile Ala Glu Ala Leu Arg Leu Gly Leu Ile Leu Ser Arg Asp 65 70 75 80 Glu Val Phe Ile Thr Thr Lys Leu Trp Cys Thr Ser Cys Glu Arg Ser 85 90 95 Leu Val Val Pro Ser Leu Lys Asn Ser Leu Arg Asn Leu Gln Met Glu 100 105 110 Tyr Val Asp Leu Phe Leu Ile His Trp Pro Leu Arg Leu Ser Ala Asp 115 120 125 Ala Gln Arg Pro Pro Ile Arg Asn Asp Gln Ile Leu His Phe Asp Thr 130 135 140 Lys Ser Val Trp Glu Gly Met Glu Glu Cys Tyr Glu Leu Gly Leu Ala 145 150 155 160 Lys Asn Ile Gly Val Ser Asn Phe Tyr Pro Lys Lys Ile Asp Glu Leu 165 170 175 Leu Ala Thr Ala Lys Ile Pro Pro Ala Ile Asn Gln Val Glu Leu Asn 180 185 190 Pro Ala Trp Asn Gln Lys Asp Leu Ile Lys Tyr Cys Lys Ser Lys Gly 195 200 205 Ile Leu Ile Thr Ala Tyr Ser Ala Leu Gly Ala Asn Gly Thr His Trp 210 215 220 Gly Asp Asn Arg Val Val Asp Ser Asp Val Leu Lys Asp Ile Ala Lys 225 230 235 240 Ala Arg Gly Lys Ser Thr Ala Gln Val Ala Leu Arg Trp Val Tyr Glu 245 250 255 Gln Gly Val Ser Met Val Val Lys Ser Phe Asn Lys Glu Arg Met Lys 260 265 270 Gln Asn Leu Gln Ile Phe Asp Phe Glu Leu Thr Glu Glu Glu Ser Asn 275 280 285 Lys Ile Ser Gln Leu Pro Gln His Lys Gly Val Lys Leu Thr Pro Val 290 295 300 Tyr Gly Asp His Asp Ala Met Lys Lys Ile Asp Asn Glu Ile 305 310 315 <210> 110 <211> 319 <212> PRT <213> Tinospora cordifolia <400> 110 Met Val Ser Val Pro Glu Val Val Leu Ser Asn Gly His Leu Met Pro 1 5 10 15 Val Val Gly Met Gly Met Ala Ala Tyr Pro Phe Gln Glu Ser Glu Thr 20 25 30 Val Lys Gln Ala Met Ile Arg Ala Ile Lys Met Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ala Leu Tyr Gln Thr Glu Lys Ser Leu Gly Asp Ala Ile 50 55 60 Val Glu Ala Leu Lys Leu Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe 65 70 75 80 Ile Thr Ser Lys Leu Trp Cys Ser Asp Ala His Pro His His Val Ile 85 90 95 Pro Ala Leu Gln Arg Thr Leu Arg Asn Leu Gly Leu Glu Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Val Ser Ser Glu Pro Gly Lys Tyr Glu 115 120 125 Phe Pro Val Arg Lys Glu Glu Leu Ile Leu Met Asp Phe Glu Asn Val 130 135 140 Trp Ala Asp Met Glu Glu Gly Tyr Arg Leu Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Ser Leu Leu Thr Met 165 170 175 Ala Lys Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro Leu Trp 180 185 190 Arg Gln Asn Lys Leu Leu Arg Phe Cys Lys Asp Lys Gly Ile Ala Ile 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Ala Lys Gly Thr Ile Trp Gly Thr Asn 210 215 220 Arg Val Met Glu Ser Glu Val Leu Asn Asp Thr Ala Asn Ala Arg Glu 225 230 235 240 Lys Thr Leu Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln Glu Val 245 250 255 Ser Ile Leu Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn Leu 260 265 270 Asp Ile Phe Asp Trp Lys Leu Ser Asp Glu Asp Leu Glu Lys Ile Asn 275 280 285 Glu Ile Pro Gln Cys Arg Gly Leu Pro Gly Asp Ile Phe Val Ser Asp 290 295 300 Asp Gly Pro Phe Lys Ser Glu Ala Glu Leu Trp Asp Gly Glu Ile 305 310 315 <210> 111 <211> 329 <212> PRT <213> Tinospora cordifolia <400> 111 Met Val Gly Val Asn Lys Glu Asn Val Val Ser Ser Val Ala Thr Val 1 5 10 15 Pro Val Val Thr Leu Asn Ser Gly His Lys Met Pro Val Leu Gly Thr 20 25 30 Gly Thr Ala Ser Phe Pro Val Pro Pro Leu Glu Glu Leu Lys Lys Val 35 40 45 Ile Met Glu Ala Met Glu Val Gly Tyr Arg His Phe Asp Thr Ala Ala 50 55 60 Met Tyr Gln Ser Glu Glu Gly Leu Gly Ala Ala Ile Lys Glu Ala Leu 65 70 75 80 Glu Lys Gly Leu Ile Lys Ser Arg Asp Glu Leu Phe Ile Thr Thr Lys 85 90 95 Leu Trp Cys Asn Asn Ala Gln Pro His Leu Val Leu Pro Ala Ile Arg 100 105 110 Asp Ser Leu Arg Arg Leu Arg Leu Glu Tyr Val Asp Leu Tyr Leu Ile 115 120 125 His Tyr Pro Val Arg Leu Lys Glu Asp Leu Leu Ser Met Asp Cys Lys 130 135 140 Glu Asp Glu Ile Phe Pro Ile Asp Ile Lys Ser Val Trp Ser Ala Met 145 150 155 160 Glu Glu Ile His Asn Leu Gly Leu Ala Lys Ser Ile Gly Val Ser Asn 165 170 175 Phe Thr Cys Lys Lys Leu Thr Asp Leu Leu Ala His Ala Lys Ile Pro 180 185 190 Pro Ala Val Asn Gln Val Glu Leu His Pro Ala Trp Gln Gln Lys Lys 195 200 205 Leu Arg Glu Phe Cys Gln Glu Lys Gly Ile Gln Val Ser Ala Tyr Ser 210 215 220 Pro Leu Gly Ala Lys Gln Trp Gly Phe Asp Val Val Leu Ser Asn Lys 225 230 235 240 Ile Ile Lys Glu Ile Ala His His Lys Gly Lys Thr Val Ala Gln Ile 245 250 255 Ala Leu Arg Trp Gly His Glu Gln Gly Ile Ile Leu Ile Pro Lys Ser 260 265 270 Phe Asn Lys Glu Arg Leu Ile Gln Asn Leu Leu Ile Phe Asp Trp Glu 275 280 285 Leu Thr Gln Asp Glu Leu Lys Lys Met Ala Ser Ile Gln Gln Ser Arg 290 295 300 Ile Ala Ile Ala Pro Glu Phe Val Phe Pro Gly Ser Pro Phe Lys Ser 305 310 315 320 Phe Glu Glu Phe Trp Asp Gly Glu Met 325 <210> 112 <211> 321 <212> PRT <213> Tinospora cordifolia <400> 112 Met Gly Ile Ser Met Pro Asp Ala Thr Leu Asn Ser Gly His Arg Ile 1 5 10 15 Pro Leu Ile Gly Phe Gly Thr Ala Ser Phe Pro Phe Pro Leu Glu Gly 20 25 30 Ser Glu Thr Ala Thr Arg Ala Ile Leu Glu Ala Ile Lys Ile Gly Tyr 35 40 45 Arg His Phe Asp Thr Ala Ala Leu Tyr Gln Thr Glu Val Gly Leu Gly 50 55 60 Glu Ala Ile Glu Glu Ala Leu His Leu Gly Leu Ile Glu Ser Arg Asp 65 70 75 80 Glu Leu Phe Ile Thr Ser Lys Leu Trp Cys Thr His Gly Arg Pro Glu 85 90 95 Arg Val Leu Pro Ala Ile Arg Glu Ser Leu Lys Asn Leu Lys Leu Asp 100 105 110 Tyr Leu Asp Leu Tyr Leu Ile His Met Pro Met Ser Phe Lys Ser Glu 115 120 125 Lys Pro Trp Phe Pro Leu Pro Gly Glu Phe Glu Ala Met Asp Phe Lys 130 135 140 Ser Val Trp Glu Gln Met Glu Ala Cys Gln Arg Leu Gly Leu Ala Arg 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Leu Ile Leu 165 170 175 Thr Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Ala 180 185 190 Leu Trp Gln Gln Asn Lys Leu Arg Glu Phe Cys Lys Ser Glu Gly Ile 195 200 205 Val Ile Thr Ala Tyr Ser Pro Leu Gly Gly Lys Gly Thr Ser Trp Gly 210 215 220 Ser Asn Arg Val Met Glu Cys Glu Val Ile Lys Glu Ile Ala Thr Glu 225 230 235 240 Lys Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Leu Tyr Glu Gln 245 250 255 Gly Val Ser Met Val Val Lys Ser Phe Asn Lys Glu Arg Met Lys Glu 260 265 270 Asn Leu Glu Ile Phe Asp Trp Glu Leu Thr Ser Lys Glu Cys Glu Gln 275 280 285 Ile Lys Lys Leu Pro Gln Ser Arg Gly Phe Thr Ala Gln Gly Leu Val 290 295 300 Ser Glu Asp Gly Pro Phe Lys Ser Ile Glu Asp Ile Trp Asp Gly Glu 305 310 315 320 Ile <210> 113 <211> 333 <212> PRT <213> Thalictrum flavum <400> 113 Met Arg Arg Pro His Ser Arg Leu Ala Ser Thr Arg Asp Met Ser Ser 1 5 10 15 Ile Val Pro Asp Val Val Leu Ser Ser Gly His Lys Met Pro Leu Ile 20 25 30 Gly Phe Gly Thr Val Ala Tyr Pro Ile Ala Ala Ser Asp Ser Ile Lys 35 40 45 Thr Ala Ile Val Asn Gly Ile Lys His Gly Tyr Arg His Phe Asp Thr 50 55 60 Ala Ser Val Tyr Gln Thr Glu Gln Leu Leu Gly Glu Ala Ile Ala Glu 65 70 75 80 Ala Leu Gln Phe Gly Leu Ile Lys Ser Arg Gln Glu Leu Phe Ile Thr 85 90 95 Ser Lys Leu Trp Cys Ser Asp Ser His His Asp Arg Val Leu Pro Ala 100 105 110 Leu Gln Asn Thr Leu Lys Thr Leu Gln Leu Asp Tyr Leu Asp Leu Tyr 115 120 125 Leu Val His Trp Pro Ile Ser Ser Lys Pro Gly Lys His Glu Phe Pro 130 135 140 Ile Pro Lys Glu Glu Leu Leu Pro Leu His Phe Glu Ser Val Trp Thr 145 150 155 160 Ala Met Glu Glu Cys Gln Ala Val Gly Leu Thr Lys Ser Ile Gly Val 165 170 175 Ser Asn Phe Ser Ser Lys Lys Leu Glu Gln Leu Leu Ser Thr Ser Lys 180 185 190 Ile Pro Pro Ala Val Asn Gln Val Glu Met Asn Pro Ile Trp Gln Gln 195 200 205 Tyr Lys Leu Arg Glu Phe Cys Lys Ser Lys Gly Ile Val Ile Thr Ala 210 215 220 Phe Ser Pro Leu Gly Ala Lys Gly Thr Ser Trp Gly Thr Asn Lys Val 225 230 235 240 Met Asp Ser Glu Val Leu Asn Glu Ile Ala Gln Ala Lys Gly Lys Thr 245 250 255 His Ala Gln Val Cys Leu Arg Trp Leu His Glu Gln Gly Ile Cys Val 260 265 270 Val Val Lys Ser Phe Asn Glu Glu Arg Met Lys Glu Asn Leu Glu Ile 275 280 285 Phe Asp Trp Glu Leu Ser Pro Glu Glu Ser Ala Leu Ile Gln Gln Leu 290 295 300 Pro Gln Ser Arg Gly Asn Thr Gly Glu Asp Phe Ile Ser Val Asp Gly 305 310 315 320 Pro Phe Lys Ser Leu Glu Glu Leu Trp Asp Gly Glu Ile 325 330 <210> 114 <211> 320 <212> PRT <213> Thalictrum flavum <400> 114 Met Gly Ser Ile Pro Glu Val Ser Leu Asn Ser Gly His Arg Met Pro 1 5 10 15 Leu Ile Gly Met Gly Thr Ala Ser Tyr Pro Phe Ala Gly Ser Glu Val 20 25 30 Val Lys Ser Ala Ile Leu Ser Ala Ile Lys Leu Gly Asn Arg His Phe 35 40 45 Asp Thr Ala Ala Leu Tyr Lys Thr Glu Gln Ile Ile Gly Glu Ala Ile 50 55 60 Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Glu Glu Leu Phe 65 70 75 80 Ile Thr Thr Lys Leu Trp Val Asn Asp Gly His Lys Asp Cys Ile Leu 85 90 95 Pro Ala Leu Gln Thr Ser Leu Lys Asn Leu Gln Leu Asp Tyr Val Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Val Cys Ile Pro Pro Gly Glu Leu Arg 115 120 125 Met Pro Gly Pro Asn Glu Lys Val Leu Pro Ile Asp Tyr Ala Ser Val 130 135 140 Trp Glu Ala Met Glu Glu Cys Gln Arg His Gly Leu Thr Lys Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Thr Cys Lys Lys Leu Glu Leu Ile Leu Ala Ser 165 170 175 Ala Lys Ile Pro Pro Ala Val Asn Gln Val Glu Val Asn Pro Leu Trp 180 185 190 Arg Gln Glu Lys Val Ile Arg Phe Cys Lys Asp Arg Gly Ile Ala Val 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Thr Ala Gly Ser Asp Phe Met Gly Asn 210 215 220 Arg Asn Val Val Gln Ser Glu Leu Leu Lys Glu Ile Ala Lys Ala Thr 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Ile Arg Trp Val Phe Glu Gln Gly 245 250 255 Val Gly Val Ile Val Lys Ser Phe Lys Glu Met Arg Leu Glu Glu Asn 260 265 270 Ile Asp Ile Phe Asp Trp Glu Leu Ser Lys Glu Asp Ser Leu Lys Ile 275 280 285 Ser Arg Leu Pro Glu Ser Lys Gly Tyr Pro Gly His Gly Phe Ile Arg 290 295 300 Val Glu Gly Pro Phe Lys Ser Leu Glu Glu Leu Trp Asp Gly Glu Ile 305 310 315 320 <210> 115 <211> 320 <212> PRT <213> Xanthoriza simplicissima <400> 115 Met Gly Ser Val Pro Glu Ile Thr Leu Asn Ser Gly His Ser Met Pro 1 5 10 15 Val Leu Gly Leu Gly Thr Ala Ser Val Pro Phe Ala Gly Tyr Glu Val 20 25 30 Val Lys Ser Ala Ile Leu Ser Ala Ile Lys Leu Gly Tyr Arg His Phe 35 40 45 Asp Thr Ala Ala Leu Tyr Arg Thr Glu Gln Pro Leu Gly Asp Ala Ile 50 55 60 Ala Glu Ala Ile Arg Leu Gly Ile Ile Lys Ser Arg Glu Glu Leu Phe 65 70 75 80 Ile Thr Ser Arg Pro Trp Leu Asn Asp Thr His His Asp Arg Ile Leu 85 90 95 Pro Ala Leu Gln Thr Thr Leu Gln Asn Leu Gln Leu Asp Tyr Leu Asp 100 105 110 Leu Tyr Leu Ile His Trp Pro Leu Ser Ile Lys Pro Gly Asn Ile Arg 115 120 125 Phe Pro Gly Pro Asp Glu Lys Ile Leu Pro Met Asp Phe Glu Ser Val 130 135 140 Trp Ala Ala Met Glu Glu Cys Gln Arg Leu Gly Leu Thr Arg Ser Ile 145 150 155 160 Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Glu Lys Ile Leu Ala Ser 165 170 175 Ala Thr Ile Pro Pro Ala Val Asn Gln Val Glu Val Asn Pro Leu Trp 180 185 190 Arg Gln Glu Lys Leu Ile Gln Phe Cys Lys Ser Lys Gly Ile Val Ile 195 200 205 Thr Ala Tyr Ser Pro Leu Gly Thr Val Gly Ser Ser Phe Gln Gly Asn 210 215 220 Asn Asn Ile Met Glu Cys Glu Val Leu Lys Glu Ile Ala Gly Asn Arg 225 230 235 240 Gly Lys Thr His Ala Gln Val Cys Leu Arg Trp Val Tyr Glu Gln Gly 245 250 255 Val Cys Leu Leu Val Lys Ser Phe Asn Glu Ala Arg Leu Lys Glu Asn 260 265 270 Met Glu Ile Phe Gly Trp Ala Leu Ser Glu Glu Glu Ala Asn Gln Ile 275 280 285 Asn Gln Leu Pro Gln Gly Arg Gly Tyr Pro Gly His Asn Phe Ile Thr 290 295 300 Pro Asp Gly Pro Phe Lys Ser Glu Glu Glu Leu Trp Asp Gly Glu Ile 305 310 315 320 <210> 116 <211> 1599 <212> DNA <213> Argemone mexicana <400> 116 atggataatt ttcttctcca gttccaacca atttcaattg cagctttttt tgttgcccta 60 gtttttctgt attggagtta tggaagaaaa agcaacaaaa ccctaaaacc aagagcacca 120 gaagcagcag gaggaagacc aataacaggt catcttcatc ttttttatgg agaagaatta 180 actcatcgaa aactaggttc catggctgat aaatatggac cagttttcaa tattagattt 240 ggttcacata aaaccctagt tgtaagtaat tgggaaatcg ttaaagaatg tttcactaca 300 aatgatcgat tattctcaaa tcgaccaggt actttagcta ttaaactaat gttttatgat 360 actgattcag taggttatgc accttatgga aattactgga gagaattaag aaaaatttca 420 actttaaaac ttctctctaa tcatagaata gaaaccctaa aacatttaag aacttctgaa 480 gttgaatcct gttttaaaca gctttataat caatgggaaa tgaaaaatga aaacagggta 540 gaagttgata aattagggtt tgcttcagta aggatggata attggtttgg ggatttaact 600 ttcaatgttg tagcaagaat tgttgctgga aaaaagaatt ttgcaggagg tgcagtaaat 660 ggtgatattg gagctcagag atataaagta gccatggatg aatcttttag attaatgcta 720 acatttgcat attcagatgt gattccatca ctgaaatggt tagacaaatt aagaggttta 780 attagagata tgaagcgttg tggatctgaa attgattcaa ttgttgctag ttgggtagaa 840 gaacatcgtt taaagagaaa ttcttctgaa aataataatt tagatcttga agaagatttt 900 attgatgttt gtttggatat tatagagagt tcttcattac ctggtgatga tccggatact 960 gtcatcaaat ctacttgtct ggatatgata ttgggtggga gtgataccac gacagtaacc 1020 ctaacatggg ccatctcctt aatattgaac aatcctcacg tgttgaaaag ggcaaaagag 1080 gaattaaatt ccgtagttgg aaaagatcga cgtgtagaag attcagacat ccctcatctt 1140 acatacatcc atgcaatcat caaagaaacc atgagactct accctgctgg accactgatc 1200 gaaaggagga caatggaaga ttgtgaagta ggtgggtacc acgttccagc tggcacacgt 1260 ttattggtta atgtatggaa gatgcaaaga gacggggatg tgtataaaga cgatccattg 1320 gtgtttagac ccgagagatt catgactagt aatgctgatg tggatctaaa gggtcagcat 1380 tttgaactga taccattcgg tgcgggtcgc aggatatgcc cgggtgtgtc atttgcggtt 1440 cagttgatgc atttggtatt ggctcgtctt ctacatgagt ttgaaattac tacaatacct 1500 tctgaaccaa aggtagatat gtctgaaagt ggaggattaa tctgtcataa gataaagcca 1560 ctagaagtgc taatcaaacc ccgactcgag ctgaactaa 1599 <210> 117 <211> 1614 <212> DNA <213> Argemone mexicana <400> 117 atggatttct catcaatact actacttctc aatattaatt gcttcacaac ttccttggtc 60 acccttcttg ttcttgtcat ggtttttctc tacaataata caagatttac taaacccaaa 120 ggtaataaaa agatgattaa accacccaga ctacccggtg cacgccctct tttaggtcac 180 ctccatctgt ttggaccagg tgagcttcct cacaaggttc tctcaaccat ggcagataaa 240 tacggtcctg cctttacaat taagttcggt aaacacacaa cattagttgt gagcgatatc 300 cataccataa aagaatgttt aactactaat gacatcctct tttctaaccg tccttcttct 360 atagcctttg atctcatgac ttacgctgat gactccgtag ctttcgcaca ttatagtcct 420 tattggcgtg agcttagaaa gatatctact ctcaaacttc tatctaataa ccgtcttcaa 480 tctatcaaac aacttcgact ctctgaggtg aacgtatgtt ttaaagaact atatagttta 540 tgcaacaata ataataaaag tggagctccg gttttgattg atatgaagaa atggttcaaa 600 gaggtcacga ctaatatagt cattagagtt attgttggta aacaaaattt tgggtctaag 660 attgtgcgag gcgaagatca agaagctgcc aattacaaga aaatcatgga tgaactttca 720 cgccttgcta aattgtctat gttctctgat tatgctcctt tacttagttt gtttgattac 780 ttccgaggaa acgtgagtgc tatgaaaaga aatggtaaag aattaaacgc gatgcttcag 840 aattggttgg aagagcataa gaggaagaag aactcatcaa tatcgaacga tgatgaacaa 900 gatttcatgg atcttatgtt gtcaattatt gacgaaacta agctatacgg acgtgacgct 960 gatactttca ttaaagctat ttccctttct atgatcgtgg gtggaataga caccgttgtc 1020 gtgactctaa catggattct cgccttaatt atgaagaatc cttttgcttt gaaaaaggct 1080 caagaagagt tagacttttt cgtcggaaag gaaagacaag tagaagattc agatctcaag 1140 aacttggtat acatgaatgc catcgttaaa gaaacgctgc gattataccc attaagttgt 1200 attcttgaac gtgatacgaa ggaagactgt gaagttggtg gctactacgt cgaaggtggc 1260 acacgtttac taataaacgc atggaagtta caacgggacc cgaatgtatg gacagaccct 1320 tcggaattta agccggagag atttctaaat gagaatgcag acatagatgt tggtggccaa 1380 cattacgaat taataccatt tggagctggt agaagagtgt gtcctggtgt atcttttgca 1440 ctccagttta tggatttggt tctggctcgt ctcatccatg ggtatgaatt gggaactcta 1500 aatggtgaag atgttgacct aactgaaaaa actgaaggac aaattaattt caaagcaacc 1560 cctctggatc tcatcgttac tcctcgtctc caccccaatc tttatgatta ttag 1614 <210> 118 <211> 1569 <212> DNA <213> Berberis thunbergii <400> 118 atggaatcaa ttgagtttct agtaactctg ctactcagcc tgcttgccct attttgtgct 60 tatgcatgga agaggaaaaa ccatacaaag gatagcaaaa tcaaagagcc accccaacca 120 gccggagcat ggccgataat cggccacctt catctaatat cccgcggcgg ccttcctcat 180 ataaacatgg gggccatggc ggacaagtac ggtccagttt tcatgatccg gctaggagta 240 aatcgagctg tgattgtgag taacacggag atcgtcaaag agtgcttcac aaccaacgat 300 aaattcctac taaaccgtcc agttgggtta gccttaaacc tcatgtgcta caacaacgcc 360 atgttcgggt tctgtcggta cggtccatat tggcgagaga tacacaaaat agtcatgctt 420 gagctcctct cgaacagccg gctcgagtcc ctgaagcacg tatgggactc ggaaatatcg 480 acatccatcg aagagttgta ccaattatgt caaacacaaa acaagaccca tgaaggccca 540 gttttggttg aaatgaagga ttggtttgca gatatggcac tcaatgtgtc ccttagaatg 600 attgctggga aaagatactt tggtgcaagt gccggtggta ataaagatga ggcaagaaag 660 tgtcaaaaga ccataagaga tttctttaga cttgttggct tgtttatagt gtctgatgca 720 cttccatttc ttaggtggct agacttggga gggcatcaga aggagatgaa gagaacattt 780 gaagatcttg actatatgct tcaaggatgg ttggatgagc ataaattgaa tagaaaatct 840 ggtgtgaatg gtgatcaaga tttcatggat gtgatgttat ctattcttga tgattcaaaa 900 tttccggatt acgacgttga caccgtcaac aaggctacgt gcttccaatt gatcatagga 960 ggaactgata ctacaacggt cactctaaca tgggcccttt ccttgatgtt aaaccaccca 1020 catgtattga aaaaggccca agatgagttg gacatccaag taggcaaaca cagacaagtg 1080 gaggaatcag acatcaagaa cctaaaatac cttgaagctg tgatcaaaga aaccttacgg 1140 atgcgcccag taggcccact gttaggccca cgagagacga tcgaggactg caccattggt 1200 ggatatcgag ttcgagcagg cacacgtgta atggtcaatg cttggaaggt gcatcgtgac 1260 ccatcggttt ggtcaaaccc tgatgacttc aaaccggaga ggtttctcaa gaaggatatt 1320 gacttcaggg accaaaactt tgaattcatc cctttcgggt ccggtagaag ggcctgccct 1380 ggaatatcat ttgccgtgga agtactgccc atggctttgg cccgtttact acatgggttt 1440 gagcttaaga ctcaattggg ttgcaaggtg gatatgacag agcatgccgg tttagttcat 1500 gctaaagcta cccctttgga agttcttgtc agcccacgcc tgtctcgaga gttgtatgta 1560 tcgtcttag 1569 <210> 119 <211> 1543 <212> DNA <213> Berberis thunbergii <400> 119 gggatcactt gctctcattg ccattattat tctttccaac attgtcaaaa aatcttcgaa 60 atctctttcc agtaaaggtg gcaagagtgc acccgtggtt catggtgcgt ggccagtcat 120 cggtcacctg cacctactga tgggtaagga gttgcctcat catgcattag ccaaactggc 180 tgataagtac gggccagcct tcacactcaa cattggcgcg tatcaagaac tagtgatcag 240 tactcgggat cttgcaaaag aatgtttcac caccaaagac aagttcttcc taaatcgtcc 300 aagcaataag gccatgaaga tcctgacata tggtgaagca tcagttgggt tcgcaccata 360 tagcccactt tgggtcgaaa tgcgcaaggt tacgaaatct aatctcttat ctcaccaaag 420 ggtcttgatg caaaatcgtt cacgagcatc agaaattgat gcaagtttta aacaactata 480 tgaacaatgc aatgggaaga gtggagcttc ggtggaaatg aaaaactggt ttgaagaagt 540 gatgttgaat gtggttacta ggaatgtttc tgggaaaaga agttttggta ccaaggcaag 600 acttggtgat gcagaggccg tgaagtataa aagggttatt gatgagaccg cacggtttat 660 ggggaacctt gtgatctcag atatggttcc aagtctttgg tggttggata acatgttggg 720 tgctgagagt gctatgaaga aacttgccat agaattggat tctctacttg gtggttgggt 780 ggaggaacat agagggaaga agttgagtga cgacgaggag ggggacttta tcagtcttac 840 atgggagatg atcgatcaga ttcagcttca tggtcttgcc cctgagacgt tcatcaagtc 900 tatgtgtgtc ggcgtgctgt tgggtgggag tgataccaca tcagtagccc taacatgggc 960 actctcgcta atgttgaaca atcgcgaggt gttgaagaag gcccaagaag aattggactt 1020 ccacgtagga aaggaccgca aagtggacga ctcggacctc aagaatcttg tttacttaca 1080 agccattatg aaggaaacaa tgaggatata ccaagtaggt cctctaattg aacgagaggc 1140 cgttgaggac tgtgaagtgg gtggattcca aatcaagaag gggaaccgta tactagtgaa 1200 cctgtggaag ttgcaacgtg accctgaagt gtggtcagat ccgtccgaat ttcgacctga 1260 aagatttctt gaggataagt ctaaggtgga cttgaagggc caaaatgctg aactaatgcc 1320 gtttggcagt ggtcgacgta tgtgtccagg agtttggttt ggggtgcact ccacttcttt 1380 ggtacttgct cgtctcatcc aagggtttga aatagagaca ccactggatg caaaagttga 1440 catgacggag acatcaagtg tgaccaatta taagggaagt cctcttgaag ttattgctcg 1500 actccccggc tcgaacctaa gctttatgat ttgtagttat gta 1543 <210> 120 <211> 1638 <212> DNA <213> Corydalis chelantifolia <400> 120 atgactccct caatttcaca accttttgag cttcttcttc ttctgatgac aatggagtct 60 cttcttctcc aatggcaacc aacttccatt gctgttcttc ttctcgccat cacaattttc 120 ctctacacct tcacaaaatc accccctaaa acccacaaaa aactagcccc agacgcaaca 180 ggaggacggc cattaatggg tcatcttcat ctcttcaatt caaacgaatt aacacatcga 240 actctaggat ccatggctga taaatatggt ccagctttca acattcgatt cggttcacac 300 aaaaccctag ttgtatctac ttgggaaatc gtcaaagaat gtttcaccac caacgatcga 360 tcattctcaa atcgcccagg aactctatac atcaaactca tgttctataa cgaagattca 420 gtagggtatg caccttatgg agcttactgg agagatcttc gtaagctatc aacactgaaa 480 ctactctcaa accatcgatt ggaatcattg aaacatttga gggtttctga aatgaatgaa 540 tgtttcaaac agctttatga gatttgtaac aaaggaagta aatctgttgc tgttagaatg 600 gataattggt ttggtgattt gacttttaat gttgttgcaa ggattgttgc agggaaaagg 660 aattttgctt caattagtaa tgatgttgga gctatgagat ataagaatgc aatggatgaa 720 gcttttagat tgatgactgt tttttcattt tctgatgtga ttccatcact tggttggttg 780 gataatttga gaggattagt gggtgatatg aaaaaagctg ggaaagaaat tgattcagtg 840 gtttcaggtt ggattgaaga acatcgtgag aagagaaaga gaagatcatc tggtggtaat 900 aacagagatg gagaattaga agaggaggat ttcattgata ttactttgtc tatcttggaa 960 acttcaagtc tccctggtga tgatcctgac atgactatca aatctacttg cttggatatg 1020 atcttgggtg gtagcgacac tacaacggtg actatgactt gggctctctc cttgctatta 1080 aacaatgctc acgctttgaa aagagcaaga gaagaactgg acttgcacgt cgggagagat 1140 agacaagtgg aagattcaga tatcaacaat ttggtctaca tccaagcaat catcaaagaa 1200 acaatgagac tatacccagc tggacccctc attgaacgga tgacaaagga agattgtgag 1260 gtaggtggat tccacatccc agccggaaca cgtttgttag ttaatctctg gaagatgcaa 1320 cgagacccaa atgtgtggga ggatcctttg gaatttcgac ctgaaagatt ccttaccaac 1380 aatgcagaaa tagatctaaa gggtcagcat ttcgaactca tcccatttgg gacgggtaga 1440 aggatatgcc caggtgtttc atttgcctta caattgatgc atttggctct cgctcgttta 1500 cttcacagtt ttgaagttgc atcccccatg gatgccaaga ttgatatgac tgaaaccgca 1560 ggccttataa gccacaaggt aattccgcta gaagtcctca tgactccacg ccttgactct 1620 aagctttatg attattag 1638 <210> 121 <211> 1572 <212> DNA <213> Corydalis chelantifolia <400> 121 acacacacaa ccgaaaagaa tttaccaccg gaagtgcctg gtgcattgcc tgtcatcggt 60 cacctccaca agttaggtgg tcggaaaggt cttctaccat atcatgttct tggagccatg 120 gctgataagt acggacctgc cttcacattc cagtttggtt ctaaccgaac ccttgtgatt 180 agtaattggg aaatggtaaa agaatgtttc acgtctacca acgataagtt attcgcttat 240 cgtccaacag cattgttcaa taaggaagta ttctgtagtg accattcttt tggattcgga 300 ccttatggcc cgttctggag ggagatgcgt aaggtttgtt cgcttcatct tctctccaat 360 tatcgcctcg agctcttaaa acactctcga aaatccgaga tggatgagtg tttcaaggag 420 ttgtaccaac tatggagtgc ttcaaatatt agcaaagaca agcaacctgc tgttttggtg 480 gtggaaatga agaaatggtt cgaagatgcg atgttcaatg ttgcttcgag agttgtggtg 540 ggaaaaggaa gtttgaaatc tcatgagaag gctggaggga gtgataatga agtagcgagg 600 aacaaatata agaaaggcat ggatgaggct agacgacata tgggaacatt tgttatatcg 660 gataggatcc cgtttctttg gtggattgat tatttacggg gtatccatag ttctatgaag 720 cgaacggcga aagaccttca tgccattgta gagacgtggt tagaggaaca ccgtcttcat 780 tgtcacaaac ggagagtatt gcaactcgat gccaaccatg aggttgaaga tatggcgaaa 840 gaggaagaaa aagattttat ggatgtttta ttgacgatgg cggacgaggg agccatcaaa 900 atctttgacc atgatcttga taccgttatc aaagctaatt gtctggatat ggttatcgct 960 ggaaccgata caccaaccgt aatgcttaca tggacccttt cactattact aaacaatcca 1020 gatgttttga aaagagttcg agatgaactg gatttacatg ttggaaaaga aagacaagta 1080 gaagaatctg atgtcaagaa tttggtctat ctccaagcag ttttcaaaga aaccctgcgt 1140 ttatacccag ctgtacctct aaatgaacgt ttgacaatgg aggattgcaa tgttgggggt 1200 tacgatatcc cagccggtac ccgtctatta atcaacattt ggaaggttca aagagaccca 1260 aatgtgtggc aggatccctc agaattccgt cctgaaagat tcttgagtaa ggaaaaatcc 1320 accatagatg tgaggggtct agattttgaa ctcattccat tcgggacagg tagacggatg 1380 tgcccaggga tttcattttc cctccagctc atgcatttgg gactcgctcg cctcattcac 1440 ggctttgaat tagcaactcc gatggatttg gatgtcgaca tgtctgcaaa tcctggagct 1500 ttcaactata aatcaaccga tcttcaagtt cttgttagcc cacggtttca ttctaagttc 1560 tacggttgtt aa 1572 <210> 122 <211> 1611 <212> DNA <213> Corydalis chelantifolia <400> 122 atggatttcc ttgctcttca atggttccca gcttccttga ctgcccttct tgccttggct 60 gtcctttaca acttctggac caagcaaagg acttacaaga atggaaagac gagtactaaa 120 caggcacctg tagctgccgg tgcatggcca gttcttggtc accttcactt gtttggtgga 180 ggtgaactgc ctcacaagat gcttgctact atggctgata aatatggacc tgccttcaca 240 atgaagtttg gtacacatca aacactagta gtcagtaaca ccaagattgt aaaagaatgt 300 ttcacaacca atgatacttt gtttgcaaac cgtccatcca ccacagcctt tcacctcatg 360 acctacgata acgagtcggt agccttcaca ccatatggtc cattctggcg tgagctgaga 420 aaaatctcaa cactcaagct tctttctaac caccgtctcc aagcaattaa ggatgtacgt 480 gcctcggaag tgaatgtgtg cttcagggag ttgtacagtc aatggaggat aaataaaagc 540 gagcctgaaa ccagtgatca tcagggtcca attctggttg atatgaagaa gtggttcgaa 600 gaagtctcag acaatgtggt gcttagagtg attgcaggga aacaaaactt tggctctaag 660 attgtgcatg gagacaaaga ggcccttcac tacaagaaaa tcatggacga gatactacgt 720 cttgcggcag tttccatgct ttcagatgtg gctcctttgc ttggttggtt agatatgttc 780 caagggcatt taagtgccat gaaacgaaat gcgaaagagg tagatactat gctatgtaac 840 tggttggagg agcatcgaac gaaaagactc tccagcgatc atgacggtgt agagcaggat 900 ttcatagatg tcatgctgtc aatcgtggag gagaacaagt tctctggcca cgacaatgat 960 accgtaatca aagctactgt cttggccatg atcatgggtg ggactgatac caccgctgtc 1020 agcttaactt ggattgtgtc cttgttgatg aacaaccgcc acgttttgag aagggcacaa 1080 gatgaacttg acttgcacgt ggggaaggat agacaagttg atgattcaga tctcaagaac 1140 ttggtttatc tcaatgctat agtcaaggaa accatgcgtt tgtacccatt aggtgctctt 1200 cttgaacgtg aaaccaagga ggattgtgag gttggtggtt tccatgtcaa aggtggaaca 1260 cgcctattag tgaatgtatg gaagctacaa cgagacccaa gcttttggac cgatccaaca 1320 gaatttaaac ctgaaagatt tttagcagac aaggcgaaca tagatgtcgg tggtcagcat 1380 tttgaactac tgccatttgg ggctggtaga agagtgtgcc caggagtctc gttcgcactg 1440 caattcatgc atttggtact tgctcgtcta cttcacggat ttgatttggc tacccctatg 1500 aatgcagatg tcgatctaac cgagagtact gaaggacatg tcaaccagaa agcatcccct 1560 ctcaatctac ttgtcactcc acgtctccat tctaagcttt atgaatacta g 1611 <210> 123 <211> 1632 <212> DNA <213> Corydalis chelantifolia <400> 123 atggagtaca cattctcatc agcctcattg ttctcctcct cctcccttca acaatggctc 60 tcaacccttc tacttctaat ttcctccatc accatttctc tttaccttac tcgtcgcggt 120 aagtcatcac cgccaatggt ccctggagca tggccactca ttggtcacct acatttactt 180 ggtgggaacg atccacttca tatcgttctt ggaaccatgg ctgataagta tggacatacc 240 tttgccatgt tattcggtaa acatccatca cttgttgtta gtagtagtga cattgtaaag 300 gaatgtttca cttctacaaa cgataaattg ttttcgcatc gtcatgttcc taccggagtt 360 aaatacatgt tctacaataa tgactcgttt ggttttgcac cttacgggcc ttattggcgt 420 gaaatgcgta aaatgaattc actttctctt ttctcacacc atcgcgttga aatgcttaac 480 cgaattcgaa catctcaaat taacatttgg tttaaaaacc tatatgaaat gtgtaccaag 540 gagaagagta ctagtacaag tagtgatgga gttgtggttg aaatgaagtc atggtttgat 600 gaaatgtttt tcaatgtact tgtgaatatg attgttaaac ctattaatga tgatgaagag 660 ttggtgaaga aatacagaga agtggcgacc gaggcaggac gcctattgtc aagcatggct 720 gtttcggata tggttccatc tctaggatgg ttggatcatt tgtttgggat tgtgggtaaa 780 atgaagaaaa ctgcaaaaga tatggatgct atcctcacta cttggttaga acagcataag 840 atcaaatctc aacagctgag gagaagtaat ggaggagaag gtgctgctga tcaaacagag 900 gaggaggaga aggagggaaa agggctcatt ggtgatttgt tgtctatgca agagtctgct 960 cttttcggcc atgaccgaga cactgtcatc aaatctgctt gccaggcgtt gatcatggct 1020 gggagtgaca gtacatcagc cacattaaca tgggttctct ctttgttact gaaccatcgt 1080 gacgcactca gaaaggctcg agaagaactg gatcaagttg taggaaagga cagacaagta 1140 gatgattcag acattaagga tcttgtatat ctccaagcca ttttaaagga aacgatgcgg 1200 ttatatccag ctgcaccgat attagagcgc ttggcagtag aggactgcat tgtaggtgga 1260 tttcatgttc aagcaggcac aacactgttt gtgaatgtca gcaagctgca acgtgaccca 1320 aatttatgga cggatccttt agaatttaaa cccgagagat ttctcaccgg ctgtaacgcg 1380 gatgtggact tgaagggtca agattacgag ctcgtgcctt ttggttcggg tagacggtca 1440 tgtcccgcgg tatcgtttgc agttcgtgta atgcttttgg tacttgcacg cttcattcac 1500 tcatttgaag tgaagactcg tgaagaagat gggattttgg atatgactga gagttcaggt 1560 cacaccaatt gcaggtctag cccactggaa gtcctcatct caccacggtt ggattcgaag 1620 atctactgtt ga 1632 <210> 124 <211> 1587 <212> DNA <213> Corydalis chelantifolia <400> 124 atggagtact actcatcact accatttctt caacaatggc cactttcatc tatatcatca 60 tcaccaactt ctattgctac aacacttctc attgtagctt gtttcaccat tgttttcctc 120 tacataaaca ccagttctaa gtccaataac aacctgaaat caccaccgaa acttcccggt 180 gcatggccat tcatgggtca cctacattta ctaggtggaa accaaccagc tcatgtcagt 240 cttgcaaaca tagcagagaa gtacaaatcg actcccttca tgatccgaat gggtcaacat 300 tcaaccctcg ttgttagttc ggttgatctc gtaaaggaat gtttcaacaa tacaaatgat 360 aagctgtttt cgaatcgtag tatcgcaaga ggaattagag atatgtttta cgatatggaa 420 tcgtttgggt ttgcacctta tggttattac tggcgtgaac tacgaaaagt atctgccctt 480 actctgttct ctactcatcg tgttgattcg gttatccaaa ttaaaacacc tcaaatcgac 540 ggttggttta agaaacttta cgagcaatgt acgaatacga aaggcggaat tgttgaaata 600 aaatcatggt tggatgaaat gatgtttgat gttcttgtga agatgcttgt tgaacctaag 660 gatgaaggat ctgtggagaa atacagacat gtagcagggg aggctttgga agtgctgggg 720 aatatgagca tctatgattt agtacctagt cttggttggt tggatcactt cactgggctt 780 gtgaagaaat ataaggtaac tggtaagaaa atggacacta tcctcaacag ttggttagaa 840 gaccatcgag gtgagaaagt agctgaggaa cacaagggtt taattggtaa gttgttgtca 900 atgaaggaag gatcaaagct tcttggccat gatggagata ctgccatcaa atctactgtt 960 caggtgttga tcctaggtgc cggcgatagt gcaggagcaa ctttaacatg ggcaatctca 1020 ttattattga accatcctca cataatggag aagcttcgga aagaactgga tgcagccgta 1080 ggaaaggata gacaaatcga agactcggat gtgaaaaaat tcacttatct actagccata 1140 ttaaaagaaa caatgcgatt atacccggtg acaacgcttc tacaacggga aacgatggaa 1200 gattgtgcta taggtggata tcatgttgct gctggcacaa gactgatggt aaatgtttgg 1260 caggtgcaac gtgacccgaa tgcgtggtca gatcctttgg agtttaagcc tgagagattc 1320 ctaactgagt gcgcgcatgt ggatttctcg ggtcagtatt ctgagcttat tccattcgga 1380 acaggtcgac ggatatgccc agctcttacg ctatcggttc ggctcatgtt attgaccctt 1440 gcccgtatta tccattcgtt tgatgtgaaa attcctaatg gggcatcgag tgtggatatg 1500 gcaacaagcg taggttttgt taatctgagg ttgaccccac ttgaagtcga actctcacca 1560 cgcttggatt caaagatcta cggttga 1587 <210> 125 <211> 1614 <212> DNA <213> Corydalis chelantifolia <400> 125 atggattcct ctcttcttca atggtacaca acagcttcaa tggctgccct tcttgcctta 60 gctttcttct acaaactctg gagtaagcca aggacttcta aaaatggaaa aagtactttg 120 caggcaccag tagcagctgg tgcatggcca gttcttggtc accttcatct gtttagtgga 180 ggtgagttac cacacaaaat gttggctgcc atggccgaca agtacggccc tgccttcaca 240 atgaagttcg gtacacaccg aacccttgtg gttagtgaca caaagattgt aaaggaatgt 300 ttcactacca atgatacgtt atttgctaac cgtccatcca ccatggcctt tcatctcatg 360 acctatgaca atgagtcggt agccttcaca ccatatggtc aattttggcg tgagttgaga 420 aagatctcca cactcaaact tctttctaac catcgccttc aggcaatcaa agatgtacgt 480 gcctcagagg tgaatttttg ctttaggacg ttgtacaacc aatggaggat aaataaaagt 540 gagactatcg gaaccggtga tcatcaagga ccaattttgg tggatatgaa gaagtggttc 600 gaagaagtgt caaacaatgt ggtgatcaga gtaattgtag gcaaacataa ctttggatct 660 aagatcgccc gcggaaaggg agaagccctt cactacaaga aagtcatgga cgaggtccta 720 cgtctagcgg cagtgtccat gctctcggat gtggcccctt tacttggttg gttcgatcat 780 ttgcaagggc atgtaagtgc aatgaaacga aatgcaaaag aagtagacac tttgctctgt 840 agttggatgg aggaacatcg aaaaaagaga acctccaacg gcagtaatgg tgtagagcaa 900 gatttcattg atgtcatgct gtccatcgtg gaggagaaca agttctctgg gcatgacagt 960 gacactgtca tcaaagctac cgtcttggcc atgatcatgg gtgggactga tacatcagcc 1020 gtgagcttaa cttggattgt ctccctgttg atgaacaatc gccacgtttt gagacgggca 1080 caagaagaac ttgacacaca tgtgggaaag gatagacaag ttgatgattc agatctcaag 1140 aacttggttt atctcaatgc tatcgtcaag gaaacaatgc gtttgtaccc actaggcgcc 1200 cttcttgaac gggaaactaa ggaggactgt gaggttggcg ggttccatgt taaggctggc 1260 acacgtctat tggtgaatgt atggaagcta caacgagacc caaacttttg gattgatcca 1320 acagaattta aaccagagag atttttaact gacaacgcaa acatagatgt tgggggtcag 1380 cattttgaac tgctaccatt tggtgccggt agaagagtgt gccccggagt ctcattcgca 1440 ttgcagttca tgcatttggt tcttgctcgg ctaattcatg ggttcgaatt ggatacccct 1500 atgaatgcaa atgtcgatat gaccgaaagt acagaagggc atgtcaacca taaagcatcc 1560 gctctcgatc tccttatcac accacgcctc cggtctacac tttatgatta ttaa 1614 <210> 126 <211> 1716 <212> DNA <213> Corydalis chelantifolia <400> 126 atgttcaagt ctgttccaag tgcacaggca attttacwya arctttcatt catccaccag 60 tacttcctgc tctctcaaac aaatatggat tcccttcttt ttcaatggtt tccagcttca 120 atggctgccc ttgttgcctt tgcttttctt tataagcttt ggagtgcacc caaaattacc 180 aaaaatggaa agacaagtac taaacaggca cccatagcag ccggtgcatg gcctgttctt 240 ggtcacctcc atcttttcgg tggaggtgaa ttgcctcaca aaatgcttgc aggcatggct 300 gaaaaatatg gtcctgcctt cacaatgaaa tttggtatgc atcgcacact cgtggtcagt 360 gataccaaga tcgtaaagga atgtttcact acccacgata ctctctttgc taaccgtcca 420 tctaccacag cttttcacct tatgacctac gatcaagagt cggtggcctt caccccttat 480 ggtcctttct ggcgtgaact acgaaagatc tccacgctca aattactttc taaccaccgg 540 ttacaagcaa tcaaggacgt tcgaacctct gaagtaaacg tttgctttag agggttatac 600 aacatatggc aaaccaacaa aaatgagcaa ggtggtactg ttagtgatca tccacgcccc 660 gttttgattg atatgaagaa atggttcgaa gaagtttcga ataatgtggt gattagggtg 720 attgccggta aacaaaactt tgggtctaaa attgtacgtg gtgaagagga ggcagtaaaa 780 tacaagaaaa tcatggatga agtcctacgc cttgcggcag tatctatgct ttctgatgta 840 gctcctttac ttggttggtt agatcttttc caagggaact tgagtgcaat gaaaagaaac 900 gcaaaagaag tagataatat gcttaatgct tgggtggagg agcatcgaag aaagaggctc 960 gcacatagtg ctaaagttga ggctactcat gtagaacaag actttgttga tgttatgttg 1020 tccatcatgg aggagaacaa gctttcatca gtccacgacg atgacactgt catcaaagcc 1080 actgtgttgg ccatgatcat gggtgggact gataccacag ctgtgagcct gacctggaca 1140 gtttccttgt taatgaacca tcgttatgtg ttgaagaagg ctcaagaaga aatcgatcaa 1200 catgtgggaa aagaaagaca agttgaagat tcagatctta agaacttggt ttatcttaat 1260 gccattgtca aagaaactat gcgtatgtac ccgttgggtg ctcttcttga acgtgaaacc 1320 aaggaggatt gtgaagttgg tgggttccat gtcaaaggtg gaacacgtct attggtgaat 1380 gtttggaagt tacaacggga tccaaatgtt tggattgatc caacagaatt taagccagag 1440 agatttttgg cagagaatgc caacatagat gttgggggtc agcattttga actgctacca 1500 tttggtgctg gcagaagggt gtgccctgga gtttcattcg cactccaatt catgcatttg 1560 gttcttgctc gcttaattca tggatttgaa ttggaaaccc caatgaatgc cgatgtcgat 1620 ctaactgaaa gtaccgaagg ccatgtcaac cacaaagcta gccctctcga tctgctcatc 1680 gccccacgac tcaactcgaa gctttatgaa ttgtag 1716 <210> 127 <211> 1602 <212> DNA <213> Corydalis chelantifolia <400> 127 atggattcct tgtttgttct tcttcaatgg ttctcagctt cattggctgc agttcttgct 60 ttggcttttc tttataacct atgggttaag ccaagaatta gtgatggtaa aggaaataaa 120 gggagtacta aacaagcacc tgttgcagcc ggtgcgtggc ctgttctcgg tcaccttcat 180 ctctttggtg ggagtgaatt acctcacaaa atgcttgcag ccatggctga taagtatgga 240 cctgccttca caatgaagtt cggtacacat cgaacacttg tggtcagtaa cacccaaatc 300 gttaaggaat gttttactac caacgataca cacttttcca accgtccgtc caccacagct 360 tttcacctca tgacttatga caacgattcg gtagctttca caccttatgg tccattttgg 420 cgtgagttgc gtaagatctc taccctcaag cttttatcta accaccgtct tcaggcaatt 480 aaggatgtac gtgtttccga ggtgaatgtt tgcttcaagg atctatacaa tcaatggaag 540 acgaatcaaa accaacgtcc ggtcttggtg gacatgaaga aatggtttga agaagtatca 600 aacaatgtgg tgattagagt aattgtgggg aaacaaaact ttgggtctaa gattgtgcgc 660 ggtgaggagg aggctgtcaa atacaagaaa atcatggatg aaatcctacg ccttgcggca 720 gtgcctatgc tttcggatat ggccccttta cttggttggt tggatctttt ccaagctcac 780 aagagtgcaa tgaaacgcaa tgcgaaagaa gtagacaaca tgcttgaaag ctggctggag 840 gagcataaaa ggaaaagact atctggtgta aatggtgcag aggaggaaga tttcatggat 900 gttatgttgt caatcatgga agagaacaag ttcactggac gtgatagcaa tactgtagtc 960 aaatctactg tcctagccat gatcatgggt ggaaccgata ctacagccgt gagcttaact 1020 tggattttct ccttgttaat gaacaaccgt catgcactga aaaaggcaca agaagaattg 1080 gatatgcacg tcgggaaaga cagacaagtt gaagattcag acctgaaaaa cttagtttac 1140 ctcaatgcta tagtcaagga aaccatgcga atgtacccac taggtgcact tcttgaacgt 1200 gaaaccaaag aggattgtga ggtaggtgga taccacgtcc gcgcaggcac acggttgttg 1260 gtgaatgtat ggaaactaca acgagaccca agcttttgga ctgatcctac agaattcaaa 1320 ccagagagat ttttaaccga gaacgcaaag acagatgttg gtggtcaaca tttcgagcta 1380 ctaccatttg gagcaggtag aagggtatgt ccaggagtct catttgcact tcaattcatg 1440 catttggtta ttgctcgttt gattcacggt tttgaattgg gtactccaat ggatgccgat 1500 gttgacttaa ccgaaagtac tgaagggcat gtcaaccaca aagcaactcc actcgatctc 1560 ctcattgccc cacgcctcga ctcaaaagtt tatgattact aa 1602 <210> 128 <211> 1593 <212> DNA <213> Chelidonium majus <400> 128 atgaggagta attacttaaa gccgacgacg acgacgatcg aggataaaag taagaacaag 60 ataagcagga agaagccaat agcagcaaaa ccaccacctg aagcattggg cgcatggcca 120 gtgatcggtc actttcttct atttacaggg aaagggttga ctcatgtgac tctaggagac 180 atggcggata aatacggacc agcatttctt attcggttcg gtagccatag aacacttgtg 240 gtgagtagtt gggagatgat gaaggagtgt ttttcggccc ctaacgataa gatcttttca 300 aaccgtgaat ccaacttatt atggattaag tcaatgttct acggttccaa ttcatatggg 360 ttctcacctt atgggccata ctggaaggag ctacgcaaga tatcgaccca aaagcttctc 420 tcccaccacc gacttgatgc gatgaagcac ttgcagatgg tagaagttga tgcctcattt 480 aaacagattt acaatttatg caacaaaaat ggaggttcac catcacctag tagtattaat 540 actactgctc tggtgaacat ggacgaatgg ttgtcacacc tgatgttcaa cgtgatagca 600 agaatggtca gtggatacca atccgatgat gtcgggacag gtgctacgag tactggggaa 660 agattcaagg tttccatgga tgagacgatg cgtcttatgg ccattttcgc tgtttcagat 720 ttggttccat ggcttgcatg tgtggatcga ttaagaggcc ttacgagaaa aatgaaccgt 780 tgtggtaagg atttggactc gataattggg agactaatcg aagagcatcg tcaaaagaga 840 cgattatgca gaaataataa aggatcaaaa aatgaagatg gcggtcatga tgatgatcaa 900 gacttcattg acatttgctt gtcaattatg gagcaatctc agcttcctgg cgataaccct 960 gaaattgcca tcaaatccat tgtgctagac atgatatttg ctggaagtca caccacgact 1020 ctggcaatga cgtggaccct ctccttattg ttgaaccatc gccacatgtt gaaaaaagcc 1080 aaggaagaaa tagatgcaca cgtgggtaat gaaaggcagg tggatgactc agacatccat 1140 aatcttgtgt acattcaagc catcatcaaa gaatccatgc ggttgtaccc gcccagccca 1200 ctgttcgaac ggatgacaat ggaggattgt gaggtaggtg ggttccacat tccagctggc 1260 acacgcttat ttgtcaatgt atggaagatg caacgagacc caactgtgtg ggaggatcca 1320 ttagagtttc gacccgaaag attcttgacg agcaagagag agatagatgt gaaaggccag 1380 cattatgaac tcataccgtt tggtgcgggt aggcggatat gcccgggcgc gtcatttacc 1440 ttgcaggtgt tgcatttggt gcttgctcgc cttatccatg gcttcgaaat gacgacgccc 1500 atggatgtaa aagtggacat ggcgtcaagt gcagggctat tcagtaacaa gatgacgcct 1560 ttggaagtgc tgatcacacc acgtacagcc tag 1593 <210> 129 <211> 1674 <212> DNA <213> Chelidonium majus <400> 129 atgaagatat tgcaggagtt tcatcaactc accatttcca atattgttct tcttctactt 60 atttctttca tctttttctc tcttagtgca tggttcattg agagtactaa ttattattac 120 agtagtaaga agaagaagag caaaaagaca ccaccacctg aggcatcggg tgcatggcca 180 gtgataggac accttcatca atttaaaccg gacgatttac ctcatgaggc tcttggagac 240 atggcggata agtacggtcc tgtttttatt gttcgattcg gtagttatag aaaacttgtg 300 gtgagtaatt cggagatggt taaggagtgt ttcacggccg caaacgatag gtccttttcg 360 aaccgtccgg ccgtcttagg gattaggatc atgctctata acaccataac gtatgcgttc 420 gcaccttatg gaccgtactg gagggagttg cgtaggatat cgtcgcagaa tctcctctct 480 aaccatcgag ttgatatggt aaagcacttg catgtcgatg aagttaatac cttgtttaaa 540 caactttacg agttatgcaa caaaaatgga ggtcgacccc ctactggtac taatactagt 600 actagtgctg ctctagtgaa catggatgac tggttgtcaa acataatgtt caacgtgata 660 gcaagaattg tcaatggaaa caacaaatcc attaataatg agaggacagg tgccataaat 720 gagaaaagat acaaaacagc tatggatgag gcgaggcgtc ttgtggcaat ttttgcagtt 780 tcagattcag ttccatggct tggatggttg gatcgagtga gaggccttat aagaggaatg 840 aacctttgtg gaaaggaact agactcaatt attgaagata taatcgacga gcatcgtcaa 900 aagagacgat tatgcacaag taaattagga tcatcaggag atgatgatgc tgataatagt 960 aatcttgagg aagaagataa cttcattgat gcgtgcttgt cgatcgagga gcaatcgcag 1020 ttacctggcg aaaatcctga aatcgtcatc aaagctttga tcctggacat gtttgctggt 1080 ggaagtgatg gcccacattt tgtgttgacg tggaccctcg ccttattatt gaaccacccc 1140 cacgtgttga aaaaggcaag agaggaagtt gatgcacacg tggcaaacga tagacacgtg 1200 gatgtctctg atatctctaa tctcgtctac atacaagcaa tcctcaaaga gtcaatgagg 1260 ttgtacccgc ccaacgcact cattgagcga atgacaacag aagattgtga ggtaggtggg 1320 taccacattc cagctggcac atacctattg gttaacgtat ggaaggtaca aagggacccg 1380 accgtgtggc aggatccctc tgagtttcga cccgaaagat tcttattaac aaacacaaaa 1440 acggagatgg agtatgaaca tataccgttt ggtgcgggta ggcgaagatg cccaggaatg 1500 aggtttgcat tgcaggtgat gcatttggtg cttgctcgac ttattcatga attcgaaatc 1560 acgacacccg tggatgtaat aaaagtcgac atgacggcaa ctaatgggtt attcaatcac 1620 aaggccgtgc ctttggaagt gctactcaca ccacgtactc ttatccgagg ttag 1674 <210> 130 <211> 1626 <212> DNA <213> Chelidonium majus <400> 130 atggattatt cagtccttct ccagtacggc tggtttgctc cttccatggc tgcccttctt 60 gccttagctt tcctttacaa tctctttttg gcttcatctc cgaaagctac tagcaagaga 120 acaataagta caaagaaacc gcccatggca gccggtgcat ggccaattct tggtcatctc 180 catctgttta aagagggtga gttgcctcac caaatgctta aatccatggc tgacaagtat 240 ggtcccgcct tcctcatgaa gttcggccaa caccgatccc tagttgtgag tgactaccgc 300 atcgtaaaag aatgttttac tactaatgat acccactttt gcaaccgtcc atccactaca 360 gctttcgatg tcatgactta cgctaatgaa tcggtagctt tcacagaata cagtccttac 420 tggcgcgagc ttagaaagat atccactctc aaacttctct ctaacaaccg tctccaggcc 480 atcaagaacc tccgagaaga ggaggtgaat gttagcttca aggggttgta tgattcatgg 540 aagaataaga acaacaagag taccggcagt gttggtgatg aacgagctcc ggttttggtc 600 gacatgaaga aatggtttga agaggtgtca aacaatgtgg tgattagggt aatcgtgggc 660 aaatgtaatt ttgggactaa gatcgtgcaa ggtgagaagg agggtgtaga gtacaagaca 720 atcatggatg agcttttacg acttgctagt ttgtctttgt tatccgattt tgcccctata 780 cttggtttgt tggatttctt ccaaggtcat gtccgtacca tgaaacgaaa tggcaagaaa 840 ctagacgtgt tacttcagag gtggttggag gagcataaga ggaagaagag cacaccagag 900 gatgagcaag acttcatgga cgttatgctg tcggttattg aggagagcaa gctttctggc 960 tatgacgctg ataccgtcat taaagctact tgtctggcca tgatcatggg tggaacagac 1020 acatccgcag tgagtctaac atggatcgtc tctttactga tgaacaatcg tcatgcacta 1080 aaaaaggctc gagaagaatt ggacgcgcag gtggggaagg atagacaagt tgaagattca 1140 gatctaaaga acttggttta cttgaatgcc atcgttaagg aaacaatgcg attatatcca 1200 ctaggtactc ttcttgaacg cgaaaccaag gaggactgtg aggttggtgg gtttcacctc 1260 gaaggtggta cacgtttact agtgaacgta tggatggtac aacgagaccc aagcgtgtgg 1320 actgatccga caaagttcac accagagagg tttcttacgg agaaggcaga catagatgtt 1380 tggggtggga attttgaact tataccattc ggagcgggta gaagggtgtg ccccggagtg 1440 tcctttgcac ttcaattcct aaatttggta ctagctcgtc tcattcatgg atatgaattg 1500 ggaactccag atgacgcgga tgtggatcta acggagagcc cagaaggaca tctcaatcac 1560 aaagcatcac ctctcgagct tctcctcacc ccacgcctca gtaaccctaa gctctatgat 1620 tattag 1626 <210> 131 <211> 1614 <212> DNA <213> Chelidonium majus <400> 131 atggagtttc tctctctaga accccaacta atttccattt ttgttcttct tcttgcttca 60 tccatattcc tctacaatct cttgaagaat catggaagga aatccaagac ctcaaaacca 120 ccagcacctg tagcatcagg tggatggcca atcatgggtc atctccatct tttcaatgga 180 agcgagttaa ctcatcaaac tctaggttcc atggctgaca aatatggtcc agctttcaat 240 atccaactcg gttctcatca aacactcgtt gtgagtagtt gggagattgt taaagaatgt 300 ttcactacaa acgaccgatt cttttcgaat cggcctggat cgttagcgat taaactcatg 360 ttctacgacg ctgattcagt tggttacgca ccgtacggtg cttactggag agatcttaga 420 aaaatctcta cgttaaaact cctttcgaat catcgagtgg aaaccctaaa acatttgaga 480 acttctgaag ctgaatcatg ttttaaacaa ctgtataatc agtggaagaa caacaaagtt 540 ggcggcgacg acgatgaatt tgctatagtg aggatggata attggtttgg agacttgaca 600 tttaacgttg tggcgagaat tgtcgccgga aaaaagaatt ttgctggagg tgcgacgagt 660 ggcgacgtcg gagctaagag atataaggaa gctatggatg aagcgttccg gttaatgacg 720 attttcgcgt tctcagatgt ggttccgtcg ttaggatggt tggataaatt gagaggtttg 780 gttggaggga tgaagcgttg tggtgcggaa attgattcca ttgttggggg ttgggtggat 840 gaacatagat tgaagagagc ttctagaaag ggaggagatc ataatgatct cgatcttgaa 900 caagatttta tagatgtttg cttggagatt atggaacatt ctactttgcc tggtgatgat 960 cctgaaattg tcatcaaatc tacttgtctg gacatgattt tgggtgggag tgacacaaca 1020 acggtgaccc taacatgggc actctcctta ctattgaaca atcctcacgt attgaaaagg 1080 gctagggagg aattggacac acacgtcgga aaggacagac aagtagacga ctccgatatg 1140 tctaatcttg tgtacatcca agcgatcatc aaagagacga tgagattgta cccagcagga 1200 ccactgatcg agcgaaggac atcggaggat tgtgaggtca gtgggttcca tgtaccggcg 1260 ggcacacgct taatggtgaa cttatggaag atgcaacgag atgggagcgt gtataaggag 1320 gatccattgg agtttagacc agagagattc ttgactagca atgctgatgt tgacctaaag 1380 ggacagaatt atgaactgat accatttggt gcgggtagga ggatatgtcc tggtgtgtcg 1440 ttcgcagtgc agttgatgca tttggtgctt gctcgtcttg ttcatggctt tgaaatgaag 1500 acggccggag gtgcaaaggt tgacatgaca gaaagtgcag gactaattag ccacaaggtt 1560 acaccgctac aagtgctgct caaaccacgc cttgcgatcc aacaagcttt ataa 1614 <210> 132 <211> 1653 <212> DNA <213> Chelidonium majus <400> 132 atggattcga tgaacctgtt tcaacaagcg attgcagtag gtacccttgt catcttcctc 60 tactatctat ggaagtggac atcaataata ttcactcgaa ctcaccaggc ggcgccacca 120 gaacctgccg gagcatggcc tattatagga cacctccatc tcctagcagg aggagcaaac 180 caactcgtat atcacatact tggatccatg gctgacaagt acggcccaat tttcacggtc 240 cgactcggca tgcgacgagc cttaatagtg agcaactccg agttagcaaa agagtgtttc 300 accaccaacg acagaatctt ttccactcgc cctagttcag tagctataaa attgatgggc 360 tacgattcag ccatgtttgg cttcgcacca tacgggcctt actggcggga gatgcgcaag 420 atagcagtaa ctgagcttct ctctaatagg cgcctcgaga tacttaaaaa tattaggatc 480 tccgagatca acatgtctat aaaagattta tatcaactct gggttgagaa taagaacagt 540 ggtgttgatg gcggcggtga cggtggtgag gtggtggtgg gatcaccggt tttggtggag 600 atgaagaggt ggtttgaaga tgtgtcgttc aacgtggtgg ttaggatggt agctgggaaa 660 agatatttcg ggagaagggt tgaaagtgat gatcgtgaag aggaggaggc gaggaggtgg 720 cagaaggcga tgaatgattt tatgcatttg gtggggattt tcgtggtgtc ggatgccatt 780 ccatttttag ggtttttgga tttccaagga tatgagaagg agatgaagaa gacggcggtt 840 gagattgatt atgttatggg aagatgggtt gaggagcacc aacggcggaa gctggagagg 900 gtggcggggg gatctgatga tcaacaggaa gatttcatag atgtgatgtt gtcgatctta 960 aaagatggtg atcagttcta tggttatgat cctgatacgg ttattaagtc ttcatgcctg 1020 tctcttgttt taggtggtag cgacacaata tcagtaacac taacatggac ggtctcctta 1080 ctgctaaaca atcgcaatgt gttagaaaaa gctcaaagtg agttggacat ccacgtaggc 1140 aggggaagac aagtggacga atcagatatc aacaatctta aatatctcca agccatcgtc 1200 aaagaaacca tgcgtttata cccagctgga ccactatcag caccaagaga ggctatggaa 1260 gactgcacca tagctggatt ccatgtccct aaaggcacac agttgatgct caatctatgg 1320 aaattgcatc gagaccctcg cgtttggtcg gatcctttgg agttcaaacc ggagaggttt 1380 cttactactg gcacccatgg agatgttgat gttaaaggac aacatttcga attattaccg 1440 tttggagctg gtaggcggat atgcccaggg atatcttacg cccttcaagt cttgcatttg 1500 acccttgctc gtcttcttca tagttttcat ttatcaacgc catatgatat ccccgtcgat 1560 atgactgaat cttctggact ttcttgccca aaagcaactc ccttggatgt cctccttaca 1620 cctcgtctcc cttcagagct ttatgtgtct taa 1653 <210> 133 <211> 1686 <212> DNA <213> Chelidonium majus <400> 133 atggatttgt tcatcttctt cagccggttc caatatattg taggattatt agctttcctg 60 accttcttct attatctatg gcgggtttca attacaggaa caaggatcaa aaccaaccaa 120 aacatcatga acggcaccaa tatgatggca ccggaagctg ccggtgcgtg gcccatagtt 180 ggtcatcttc ctcagctagt aggccctcag ccactcttca agattcttgg agacatggcc 240 gacaagtacg gttcgatatt catggttcgg ttcgggatgc acccaacctt ggttgtgagc 300 agttgggaaa tggcaaagga gtgcttcact accaatgaca agttccttgc tagtcgtcca 360 actagtgctg gaggcaagta cctcacctac gactttgcta tgtttggctt ctcattttat 420 ggcccctatt ggcgtgagat ccgtaagatc tccacgctcg aactactatc ccatcgtcgc 480 gttgagttgc tcaaacatgt cccttacact gagatcggcg gctctatcaa acaactttac 540 aaactttgga tggaaacaca aaaccaaaat aagcaacggg atgatcatca ggtaaaggtc 600 gacatgagcc aagtgttcgg atatctaaca ttgaatacag tgttaaagct agtggtagga 660 aagggtctat tcaacaacaa tgacatgaat catgaacagg aagaaggtcg aaagctccac 720 gagacagtac ttgaattctt caaactggca ggggtctcag ttgcatcgga tgctcttcca 780 ttccttggct ggctggatgt agatggacag aagagaagca tgaagaggat agccaaagaa 840 atggatttaa tcgctgaaag atggcttcaa gagcatcgac agaaaagact aacatcaaac 900 aacaaggcta gcagcggcca tgacgacttc atgagcgtgt tgctatccat ccttgacgac 960 gattccaatt tcttcaatta caatcgtgat actgtcatta aagccacctc tctgaacctg 1020 atattagcag cttcagacac tacatcagtg tccctgactt gggttctctc gttattagtc 1080 accaaccccg gtgccttgaa aaaggtccaa gatgaactgg atactaaggt gggcaggaac 1140 agacatgtcg aagaacgaga catcgagaaa ctggtctacc tccaagccac agtcaaggaa 1200 actctacgga tgtacccagc tggtccacta tcagtacccc acgaagctac ccaagactgc 1260 accgttggtg ggtatcaggt aacagcaggc acacgtttgg tggtgaacgt gtggaaattg 1320 caacgcgacc cgcgtgtgtg gccaaaccca tctgagttca agccggagag atttctacca 1380 gacgggtgtg aggttggttg tggtgaagca gcaaatatgg atttcagagg acaacatttt 1440 gaatacatac catttgggtc aggaagaagg atgtgtccag ggatcgactt tgccatacag 1500 atcatacaca tgacactggc ctgcctactc catgcattcg agttccaggt gccctcatca 1560 ctagataaac atcttgtacc tgcagtaatc gacatgagcg aaggttcagg actcaccatg 1620 cccaaagtaa ccccacttga agtcctcctt aacccacgcc tacctcttcc gctttatgag 1680 ttataa 1686 <210> 134 <211> 1569 <212> DNA <213> Cissampelos mucronata <400> 134 atggaggcca tgggtgaata ttttcagctt atttcatgga ctgtgttttc ggctttgatc 60 accatctact ttatattcag taggaagact aatggaaaaa agaaggcacc ggaaccaggt 120 ggtgcatggc cgattattgg tcacctccat ctttttggag ctcatgacct gttgtaccga 180 aagcttgggg ccatggccga caaactcggc ccagtattca tgatccgttt tggcatgcat 240 cgagccgtcg tcgtaagtaa ctatgaagta gcaaaagaat gcttcactgt caacgataag 300 atactcgcaa gtcgcccacg gattattgct tcaaaactca tgggctacaa ccatgcggcc 360 gttggattaa gcccttatgg cccttattgg cgagaggcac gtaagatagc caccttggaa 420 cttctgtcta accatcgact ccaactactc aagcacatca ggatatcaga gattgatatg 480 tggataaaag agctaaaaga attttgtatg aaaagcagta gcgatggtcc agttcttgtt 540 caattagatg gatggttcaa tgctttgaca cttaatataa tggttaggaa tatagccggc 600 aagcgatatt gtggcggtgc tgaagccttt gaagatgaag agtcgcagcg atggaagaaa 660 gtttctagag aacttatgtt cttgttcgga gtctttatgg tacccgatgc atttcctgta 720 ctggaaggca tagatgtgca gggtttcgag cgagccatga aaagggttgg taaggaaatg 780 gatttcttct tgagtcgatg gttggaagag catagaagga agaaaataga actctccgag 840 aaaggaaacg aagttaatga tcaaggagct caagatttca tagacgtgat gctgaacaca 900 atcaaggatg ccaagatctt tgagcatgac gctgatacga tgatcaaagc cacttgtatg 960 gctctagttg tagcagggaa cgacacgaca atgatcactc tatcatgggc agtttcattg 1020 ttgctgaata atcgtggagt tttagagaaa gcccaggagg aattgagcaa ccagatagga 1080 aaaaacaggc atgttgagga aggagacatc gagagcttgc cttaccttca cgcaattgtg 1140 aaagagacct tccggctata ccctgctgca ccactttcat taccacatga agctatggaa 1200 gattgcacca ttgccggctt tcatgttcct gcaggaacac gcttgattac taatatttgg 1260 aagctgcata aagatcctaa tatttggcct gacccattgg agttcaagcc agagagattc 1320 ctcactactc atgcccatgt cgattttaaa ggtcaacact tcgaattcat ccctttcgga 1380 tcaggaagaa ggatgtgccc tggatcgtcg ctagcaatca gtgttttgca cctcaccctt 1440 gcacgtttgc tccatgaatt cgagcttaaa actccggatg atgcaccggt ggacatgacc 1500 gaaggtccgg gtatcacact catgagagca aacccccttc acgtgctcat taatccaaga 1560 catgactga 1569 <210> 135 <211> 1605 <212> DNA <213> Cissampelos mucronata <400> 135 atgtcctcgc ccatccaaca actactcctc tccttgacct ccattaatgg cggaaccatc 60 atctcagccg tcataatatc actccttctc atcctcaccc ttcatcgcct cctaaaaccc 120 attaattcca acaaaaccaa acaccctcca agagcatccg gggcatggcc cgtcatgggc 180 cacctccaca agctccgcgg gccggacctc ccccaccgca ccctctcatc catggccgac 240 cagtacggcc caatcttcac aatcaatctg ggccccacca ccgccctcgt catcagcgac 300 ccagccgtcg caaaagagtg cttcaccacc aacgacctca ccttctcctc tcgaccgacc 360 actctcgcca ccactctcat gtgctacaac aacaccatgt tcgggttcgc cccgtacggc 420 ccgtactggc gcgaagtccg caaggtcgtc atggcccaac tcttatcaac ccgtaggctc 480 gagtcactca aaaacatttg ggcctcggag attgaccgct gggttaaggg cctggcccaa 540 aaggcccaaa gtgggtcgca tggtgtggtt gaggtggaga tgggtgattg gctggcgagg 600 ttgactatga acattgggct aaggttggtc gtggggaaga gttgtggtga gatggggagt 660 gaggagtcgg agaggtgctt gggtgctatg cgggagtatc cggagttgct gggtcggttc 720 gcgttggagg acgcgttgcc gtggttgggt cggtttgatt tgcaagggca tcagagggag 780 atgaggaggg ctgcgagggt cttggatgag attcttgatg ggtggttgga tgagcataag 840 cgaaatagat cggggttaag tggtggtgat gatcataggg atcaggattt tatggatgtg 900 atgttgtctg ttcttgaaca agatcaagat tcgagtctta gcgaccatga tgctgatatg 960 atcaacaagt ccacttgcct gaatctaatc ttaggcatgg ctgataccac aatggtggcc 1020 ttaacatggg ccatttctct tctcctcaac aacaaaacgt ccctcaagaa ggcccaagaa 1080 gaattacaag cccaagttgg caatgaaaga cacgtggacg aatctgatgt caagaaccta 1140 gtctacctcc aatccatcat aaaagaagtg ttgaggcttt acccaccaga gccactatcg 1200 ggcccacgag aggcccttga agactgcgag gtggcaggat accatgtccc gcgaggcact 1260 cggcttatcg cgaatttgtg gaagatccat cgtgactcgt ccacgtggca ggattctatg 1320 gagtttcgac cggaaaggtt tctgacggcc cacgaacacg tggatgtgtg gggccagcac 1380 ttcgagttca tgcctttcgg gtcgggtcga aggtcgtgtc cgggagtctc gtttgaggtt 1440 cgggtgttgc cattgatact ggctcgtttg atacacgcat tcgagatgac gacacgtgga 1500 gatgtgccgg tggatatgag tgaaagaccg ggccttgtta tctcaaaagc aagcccactt 1560 gaagttatga tttctccgcg cttgcccctt cacttgtttg aataa 1605 <210> 136 <211> 1611 <212> DNA <213> Cissampelos mucronata <400> 136 atggagtttc atttgcttct ccaagcagtt gcagcaactc tagtgacctt cttcctttat 60 gagctatggg ctctaaggaa gaagttcagc aggcttacca aaactccctc atcaaaggct 120 ttgctcaaag caaagtgcgc accagagccg cccggtgcgt ggccgatcat cggtcacctt 180 cctttgctgg tttcagccaa gcagcctcat cgggcatttg ctgcacttgc tgagaagtat 240 ggtccagctt tcatgctccg catgggcatg agtcctatgc tgattgtgag taccaaagag 300 gttgccaaag aatgttacac cgccaatgat catgtgtttg ctactaggcc tgtcaccacc 360 gctgggaagc tcatggctta tgatcactcg gtcatgggct tcactccttt tggaacctac 420 tggagagaaa tacgaaagat cgcgacggtg gagctgttct cggctcgcag gatcgggatg 480 ttgaagccgg taagacagtc tgaggtctcg ttgtggatga agggattgca tgagaagtgg 540 gtgcataatg gaaatagctc ggtctccgtg gagctgaaga gtcaactgga agaactcact 600 ttcaatttgc ttacacaaat ggttgccgga aagcgttact acggaagtaa cgttgcaaag 660 gtggatgaga agatggcggg gctatttcgc cacgccgtcc aacaattcaa ttatcacctt 720 gggaactcgg agatgtatga tgccttgcca ttcatggcat ggttggactt caagggcgac 780 gccaaggcca tgaaaaagac acaaaaggac ttggatttta ttatgcagac ttggttggac 840 gagcaccgcc taaaagcgga tcaaatgcgc ggggacgcca tcaacaacac cagagacttc 900 ctcgacgtgc tggtgatgat ggagaagacc ggccaatttt catcggcaat taaagacaga 960 gacactacaa ttaaggcctt ggctctgacc caactagtag ccggagtgga cagcatggcc 1020 aacacaatgg tgtgggtgtt ggctctccta atgaacaacc cagaaatgct agccaaagca 1080 caagacgagc tcgacaacaa cgttggcaaa gacagactcg tggaggagtc agatatccct 1140 aatttgaagt acttacaagc cctcctaaag gagaccctcc gaatgtaccc cgtggggcct 1200 ttgttggtgc cgcacgaggc aacggaggat tgccacgtgg ccgggtattt tgttccacgt 1260 ggaactggcc tgttcatcaa cgcttggacc atacaacgtg acccaaatgt atgggctgag 1320 ccgaactgtt ttaatccaga gaggttctta accactcatg cagagatgga cgtgaagggt 1380 caaaatcatg agctgttgcc attcgggtcg ggtaggcgat cgtgcccagg tgtgggactg 1440 gccctccaag tgatgcacct caccttggct cgaatattac aagcgtttga gttgaagact 1500 caatcgggtg ttggaattga cttggaagag tgctccggca tactgctttc catgatgcac 1560 ccattgcaag tgatgatggc acctcgcctt ccttccgaac tctatgattg a 1611 <210> 137 <211> 1575 <212> DNA <213> Eschscholzia californica <400> 137 atggattcct taatccttac caattggttt ccaatatcaa ttgcttctgt tttaacccta 60 gttttccttt acaaagtctt actttcctca agaactctta aagataagaa gattaagact 120 tcacccatgg caaatggtgc atggccaatt cttggtcatc tccatctctt tggttcaggt 180 gaattacctc ataaaatgct agctaccatg gctgacaagt atggctcagc cttcaggatg 240 aagttcggta aacacacaac actagttgtg agtgataccc gtatcgttaa agaatgtttc 300 actaccaacg ataccctctt ctctaaccgt ccttccacta aagcttttca actcatgact 360 tacgataatg agtcggttgc ctttacacct tacggtcctt actggcgtga gcttagaaag 420 atatccactc ttaaacttct atccaaccat cgtctccaag ccatcaagga cgttagagcc 480 tcggaggtaa acgtatgctt caaaagctta tacgatcagt gtaaaaatcc aagtggagct 540 cctattttga ttgatatgaa gaaatggttc gaagaggtgt cgaacaacgt ggtgatgagg 600 gtaattgtgg ggagacaaaa ctttgggtct aagattgtgc aaggtgagga ggaagctatc 660 cattacaaga aggtcatgga tgagctctta cgtctcgcta gcttgtctat gttctcggat 720 tttgctcctt tacttggttt cttggacatc tttcaaggga acttgagtgc catgaaacaa 780 aacgccaaga aggtagacgc aatccttgag aactggttgg aagagcatcg gaagaagaag 840 aactcagttg ctgaaagcga gcaagatttc atggatgtta tgttgtcaat tgccaacgag 900 agcaagttgt ctggtcacga tgccgatact gtcattaaag ctacttgcct agcaatgatt 960 atgggtggaa cagacactac tgctgtaagt ctaacatgga tcatttcttt attaatgaac 1020 aatcgccatg ctttgaagaa agctcgagaa gaattagatg cactagtagg aaaggacaga 1080 caagttgaag attcagattt aaagaattta gtatacatga atgctatcgt taaggaaacg 1140 atgagaatgt acccgttagg tactcttctc gaacgcgata ctaaggagga ctgtgagatc 1200 ggcgggttcc atgtcaaagg tgggacgagg ttgctagtga atgtgtggaa gttgcaacga 1260 gacccaaatg tatgggttga tccaacagaa tttagacctg aaagatttct aaccgagaat 1320 gcggatatag atgttggagg tcagcatttt gagttgctac catttggagc aggacgaagg 1380 gtgtgtcctg gggtgtcgtt tgcactacaa tttatgcatt tagtacttgc tcgtctcatc 1440 catggatacg atttgaatac tctaaacgaa gaaaatgtgg atctgacgga gagcccagaa 1500 ggacatgtga accacaaagc atcgcctctt gatctcatcc tcacccctcg tctacattac 1560 aagttgtacg aatag 1575 <210> 138 <211> 1599 <212> DNA <213> Eschscholzia californica <400> 138 atggatactg cttcatcaat ttcccttctc ctcccttggg cagtagtagt tccctccata 60 gctacccttc ttgctttgct acttttcctc ttcatcactt caccaaaaac acccaagagg 120 aaaacaagtt caaaaccacc acccactgtt cttccaggtg cctggccaat tctaggtcat 180 ctccatctct ttaaagaagg agaatcacct caccacatgc ttaagaactt agctgataaa 240 tatggacctg ctttcgtcat gaaattcgga caacatcgtt ccctagttgt aagcaacact 300 aaaattgtta aagaatgttt tactactaat gacaccctct tttccaaccg tccatctact 360 actgcctttg atctcatgac ttatgctcat gactcggtag ctttcacacc ttatagtcct 420 tactggcgcg agctaagaaa gatatcgacc ctcaaacttc tatctaacaa ccgtctcaaa 480 gcaattaaga agctccgagg agaggaagtg gacgtttgtt ttcgtgggtt gtacggttta 540 tggaagaata aaactaaaaa tggtgctcca gtcttggttg acatgaaaaa atggtttgaa 600 gaggttgcaa ataatgtggt gattagagtg attgtgggga aacttagttt tgggaccaag 660 attgttgatg gtgaagaaga agccgttgaa tataaaacag tcatggatga actcttacgt 720 cttgctagtt tgtctttgtt atccgatatg gctcctatac tcggttggtt ggatttcttc 780 caaggaagtg ttcgtaaaat gaaacaaacc ggtaaaagac tagacgtttt acttgagaaa 840 tggttggggg agcaccgaga gaagaagaac ttggttgggg aagatgagca agatttcatg 900 gatgtcatgt tgtcgatcgt ggaagaaagt aagctatctg gccatgaagc tgatgccgta 960 attaaagcta catgcttggc catgattatg ggtggaacag acacaacagc agtaactctg 1020 acatggatca tctccttatt gatgaacaat cgtcacgcac tagaaaaagc tagagaagag 1080 ttggagacgc acgtcggaaa agatagacaa gtggaagata cggatttgca gaacttggtc 1140 tacttgagtg ctattgttaa ggaaacaatg cgattgtacc ctttgggtac tcttctcgaa 1200 cgagaaacta aagaagactg cgaggttgga gggttccaca tccaaggtgg aacgcgttta 1260 ttggtgaata tatggatggt acaacgagac cccaccgtat ggaatgaccc gtcggcattt 1320 aaaccagaga ggttcttaac agataaatcg gaaatagatg ttgggggtca acatttcgaa 1380 ctgataccat ttggggctgg tagaagagtg tgtcctgccg tgtcgtttgc tcttcaattc 1440 ttgcatttgg ttctcgctcg actcatccat ggatatgact tgggcactcc aagcaacgtg 1500 gaagtagact tgactgagag cctggaagga cacgttaatc ataaagcatc tcccctcgaa 1560 ctcctcctca ctccacgcct caacccgaag ctttattaa 1599 <210> 139 <211> 1647 <212> DNA <213> Eschscholzia californica <400> 139 atggaatctg agatccaata tcaaactgca acaatggctt ccattcttag tactaatttg 60 attctaagca gtatttttac tgcttttctt ctctatttct tactaaagat gagtccattt 120 aggtcatcaa aaaacaagtc aagaaagcaa gcaccaaaac caactggttc atggcccatt 180 ataggtcatc tccttcatct ccaaggacca aaccttcctc acataaactt agcagctttg 240 gctgataagt atggatcagt gttcagcttc cgtattggtc tccgtccagc cctcgttgtc 300 agttcttggg agatagctaa agagtgtcta actacaaatg atagagtctt cgtctctcgc 360 ccgccgttaa tagctatgaa acacatgggt tatgaccacg ccttgttcgg attctcccct 420 tatggtccat actggcgcga actccgtaaa ctagtgaacc aagaacttct ttcgaacact 480 aggattgagt tgataaaaca tgtttgggat actgaaataa atacattcat taatgacctt 540 tatgaagttt gggctatgaa aagtaatgaa ggtgggggtg atgttgtggt tgaaatgaag 600 caatgcttgt acgattttac tctcaatctc acactaaaaa tacttaccgg aaaacggtat 660 ttcggtggtg gtggcagcga agagttaaga gaagaagcag gaagatgtca aaaagcagta 720 agaaactttc ttaggttagt gggtactttt acagctcaag atgtcattcc atttttggaa 780 ggttggttag acttgggtgg tcatgagaag gagatgaaaa ttacaggaaa agaactcgac 840 tccctacttc aaaaatggtt agaagaacat aaagtaaaga aatcgtcagc tggggaagaa 900 ggccaggaga atcgaggtgg tgatgaagaa gatttcatgg gggtgatgct tacaaaactt 960 cgtgatcacg agaagctttt aagttattac gatgctgata ctatcaacaa ggctacttgc 1020 ttgactgtaa tcctaggtgg gagcgacacc acgatgatga ctctagtatg ggctctcgct 1080 ttactagtaa atcatccgca tgtaatgaag aagctccaag atgagttgga tacccacgta 1140 agtaaagaaa gacaggtgga agaatcagat atcaagaacc tcgcgtatct tcagtctgtt 1200 atgaaggaaa caatgcgtcg ttacccaggg acccctctat atgttagaga atcgatcgag 1260 gattgcacta tagctgggta cgacgtccca acaggaaccc gtcttgtagt gaatgcgtgg 1320 aagatccaac atgacccaca agtatggtct aacccattcg agttcaatcc agagagattc 1380 cttacaaccc acaaggacat agatgttaga ggtcagaatt tcgaacttat cccatttgga 1440 gcaggcagga gaatgtgccc cggggcttca ctcggtcttc aagtggtgca cctgactctg 1500 gctcgtctag tacatggatt tgagtttaaa acaccagatg gcgaacccat ggatatgacg 1560 gagagtatag gactaactaa tcttaaagcc actccacttg aaattatgct cactccgcgc 1620 ctctcttcca aactatatgt gtgttga 1647 <210> 140 <211> 1665 <212> DNA <213> Eschscholzia californica <400> 140 atgggtcttt catttgatct ttcttctcat aactttcttc aattctcgaa ccccactatt 60 ctcattggcc tctttggttt actagtttac cttttactag taaatcttcg aaaacgaata 120 tcacgaaaaa atgaggcacc cgaagtggag ggtgggtggc ctatcatagg tcatcttcat 180 catttcatgg gtgggaaaaa caagctactc catgtagcat ttggagcttg ggctgataag 240 tatggaccag tctatactct gaggatgggt ctgaataaag tactagttgt gaatagtgca 300 gaagtagcaa aagagtgttc aaccactaat gacatgcttt ttatggctcg tccatatcga 360 atagcctctg agatcatggg ttatggatat gcgatgtttc ctatcgctcc ttatggacca 420 ttctatctca aaatgcgaaa aatggtcacc caagagcttc tctcaaatag tagggttgat 480 tctctaaaac atgtgtgggg ttctgagata aaaaatgcca ttcaagaact tcacaagaaa 540 gtattatcaa ctaaaggtgg tggtccaatt tcaatggaca tgaagggatg ggtttccaat 600 ttaacgttcc gaacggctat gaaggtgatt tgtggtggtg ttggtgttga tggtggtggt 660 ggtggtggtg gtgctacttc tcctgcaact agtattggag aacataatta tgatgaagtt 720 ggaagttttc aaaaagcatt gaaagaattc tttgttttac taggagaaat taggatttct 780 gatgtgatac cattcctagg gtggttagat atgcgaacag ggtacgtgga aaagatgaag 840 gacaatggaa aatttctaga caatttaatg gaggaaatgt tggaagaaca caaaaggaag 900 agaaggtcac taactgaaga agaggaaaaa gatggaggtt atgtggagca agatttcatg 960 gacgtaatga tatcgaaact taatgatcca aagcttctgt cctattatga cgctgatact 1020 atcaacaagt ctacttgcct gactctaatt ttaagtggga gtgagacaac gatggttagt 1080 atagtttggg ccttagcgtt actagtcagt catccacacg tgttaaagaa agcgcaagat 1140 gagttggata cgcttgttgg tcgggaaaga caggtagacg aatctgatat caaagatttg 1200 gtatacctcc aagctgtagt taaggaagca ttgagactat ttccaccggc tccactttca 1260 actccacgcg ttgcaacaga ggattgtgtc gtatcaggat atcatgtacc tgcagggacc 1320 caactttttg taaatacttg gaagattcaa cgtaacccgg aagtatggcc tgaaccatca 1380 gagtttagac ctgagagatt tttcacaacc cacaaggatt tcgacgttag aggtctacat 1440 tatgacctcc accctttcgg gtctggtaga agagcatgtc caggtgctgg cttcgccctt 1500 caagtggtgc atctaaccct agcttctcta ttacatgggt tcgagattaa aaacccatcg 1560 gatgaaccca ttgatatgac tgagagtcca ggagtaacta acttgaaagc aaccccactg 1620 aaagtcctcc tgacccctcg cctcttctct aaagaagttt attaa 1665 <210> 141 <211> 1686 <212> DNA <213> Eschscholzia californica <400> 141 atggagaagc ccatcctcct ccagctccaa cctggtattt taggcttatt agctttgatg 60 tgcttcttat attatgtaat aaaggtttca ttatctacaa ggaactgtaa ccagttggtg 120 aggcatccgc cggaagctgc aggttcatgg cctatagttg gtcatcttcc tcaactagta 180 ggctctggta agcctctgtt tagagttctc ggagacatgg ctgacaagtt tggaccaatt 240 ttcatggtgc gatttggagt acacccaacc ttggttgtga gcagttggga gatggcaaag 300 gaatgcttca cctccaacga caagttcctg gctagtcgcc cacctagtgc tgctagcatt 360 tacatggcat acgaccacgc tatgcttggc ttctcctctt atggcccata ttggcgtgag 420 attcgtaaaa tatcaaccct ccacctactc tcccatcggc gccttgagtt gcttaagcac 480 gtccctcact tagagatcca caattttatc aagggattgt acggaatttg gaaagatcat 540 caaaaacagc agcagcagcc tactgcgaga gatgatcaag attcggttat gctggagatg 600 agccaattat ttgggtactt gaccttgaac atcgtattga gcttagttgt aggaaagagg 660 gtttgcaact accacgctga tggtcatctt gatgatgggg aagaagctgg ccaaggtcaa 720 aagctccatc agacgataac tgatttcttt aaactgtcag gggtttcagt ggcttcggat 780 gctcttccgt tcttaggctt gtttgatttg gatggacaaa aaaagatcat gaagcgggta 840 gccaaggaga tggactttgt tgcagaaaga tggcttcaag ataagaaatc gagtttgttg 900 ctgtctagca aaagcaacaa caaacagaat gaggcagggg aaggcgatgt ggacgatttc 960 atggacgtat taatgtccac actcccagat gatgacgatt cctttttcac gaagtatagt 1020 cgagatactg ttattaaagc caactccctc tctatggtcg tagcaggctc agacacgaca 1080 tctgtttctt tgacatgggc tctttcctta ttactcaaca acatacaagt cctaagaaag 1140 gctcaagatg agctggacac gaaagtcggt agggacaggc atgtagaaga gaaagacatc 1200 gataacttgg tctatcttca agctatcgtc aaggaaacac tccgcatgta ccctgccggt 1260 ccactttcag taccacacga agccattgaa gactgtaatg ttggcggata ccacataaaa 1320 acaggaaccc gtctgctggt gaacatatgg aagttgcagc gcgacccacg agtgtggtca 1380 aatccatctg agttcaggcc agagagattt cttgacaacc aaagtaatgg aacactgctg 1440 gatttcaggg gtcaacattt tgagtacata ccatttgggt ctgggcggag gatgtgccca 1500 ggcgtgaacc ttgcaacccc gattcttcat atgacactag cacgcttact acaatcattc 1560 gacttgacca caccctcgtc atccccagta gacatgactg aaggttcagg attgacaatg 1620 cccaaagtaa ctccacttaa agtactactc accccacgcc tccctcttcc actttatgat 1680 tattag 1686 <210> 142 <211> 1698 <212> DNA <213> Eschscholzia californica <400> 142 atgaatttgt ctagcaaatt gatgggttca tttgatcttt tctctcttaa ctttctccag 60 ttctcaaaac ccgccagtat ggttattatt ggcatttgta gttttctagt ttacttttca 120 ctactaaatc ttgtaaggcg agtactagca ccgtcttgta cgatgaaaaa cggaaaaact 180 gagccaccgg aggtggaaaa cgggtggcct attataggtc atcttcacca tttcatgggt 240 aaaaagaata agctaatcca tgaaatattt ggagacatgg ctgataagta tggaccgacc 300 tttactttaa ggatgggtct gactaaagtt ctagtagtga gtagtgccga agtagcgaaa 360 gagtgtttaa ctacaaacga tttggtattc attggtcgtc cacctcgtgt agccaattca 420 ctccttggtt atagttttgc catgtttcct ttttcgcctt atggcacata ctatagccaa 480 atgcgaaaaa tagtcaccca tgagcttctc tcaactagta gggttgagtc tctaaaacat 540 gtgtggaatt ctgagataaa caaagccatt caagaacttc atcacaaagt agtatcagtt 600 ggaggtggta gtcctgtttt aatagaattt aagcgttggt tttcggattt aacgttaaga 660 actactgtta agttgatctg tgggaagcag tactttggta cggatggtgc tactcaggcg 720 agtatgacta ttaatggagg aggagatgat gatgaagctg ggaagtttca agaggccttg 780 agagagttct tttgtttact aggaaaattt agggtttctg atgtgatacc atttttaggg 840 tggttagatt ttggaacagg ttataaggaa aagaaaaata gaatgttcat tgatagttta 900 atggaggaat ggttggaaga acacaaaatg aagagaagat tactaaatga agcagataaa 960 aaagaaagtc gtatagagca agatttcatg gatgtaatga tctcgaaact tgatgatcct 1020 aaccttctat cccattacga tgctgatact atcaacaagg ctacttgctt gactttgatt 1080 ttagggggaa gtgacacaac aatggttagt ttggtttggg ccttaacatt gttaatgaat 1140 catccacatg tgttgaaaaa ggtccaagat gagcttgatt tccacgtagg tcgtgagaga 1200 caggtcgaag aatctgatat gaaaaactta gtatatctcc acgctgtaat gaaagaggca 1260 atgagactaa atccagcagg tacgttatcg gctccacgta tgtcaacaaa ggactgcacc 1320 gtatccgggt accacattcc tgcaggaacc catctattca tgaatacttg gaagattcaa 1380 cgcgacccta atgcatgggt tgaaccaaca gagttcagac cagagagatt tctcactacc 1440 cataaggatt tcgatcttag agggcagaac tttgaactcc tccctttcgg gtcgggtaga 1500 agatcatgtc ttggtgctaa ctttgctctt caagtgttgc gccagactct agctcgcttg 1560 ttacatggat tcgatctcaa aactccatca gatgagcccg tcgatatgac tgggtctgct 1620 ggactaatta atatgaaagc cactccactc gaagttctag tcaccccacg cctcttctct 1680 agtgaattat atgggtaa 1698 <210> 143 <211> 1853 <212> DNA <213> Eschscholzia californica <400> 143 atgctcataa aacctctcgg gagcaagata acatctcctc ctccctccag tgtcaaagaa 60 aaaagagaaa tccgaagggt cgtcatgagg tatataagtg ggcttaaacg atgcaaaatc 120 agcaagataa agccaattcc aagatgtaac caaaacattc tcgcatttaa tatcaccatg 180 acaaacacca agtatcttcc aatgaatttg ctcatcttct tccaattcct cctccaattc 240 caagttcttg taggattatc agttttgcta gccttctcct attacctatg ggtttcaaaa 300 aatcccaaaa tcaacaaatt taagggtaag ggtgccttat tagcacccca agctgccggg 360 gcatggccta ttgttggtca tctccctcaa ctagtaggcc ccaagccgct cttcagaatt 420 cttggagcca tggctgacaa ctatggacct atcttcatgc tccgattcgg ggttcatcca 480 actgtcgtcg tgagcagttg ggagatgaca aaggaatgtt tcactaccaa cgataggcac 540 cttgctagtc gtccatctaa tgctgcttcc cagtacctta tctacgaagt ctatgctttg 600 ttcggcttct ccttatatgg tagctcatat tggcgtgatg ctcgtaagat tgctacgctc 660 gaactactct cccatcgtag gcttgagttg ctcaagcatg taccttatac ggagatagat 720 acatgtatca aacaattgca cagactttgg acaaaaaata acaaaaacca aaacaaccct 780 gagcttaaag tcgaaatgaa ccaatttttt actgatctaa ccatgaatgt gatattgaag 840 ttggttgtag gaaagcggtt tttcaacgtt gacgatgcag cggatcatga aaaagaagaa 900 gctcgaaaaa tccaggggac aatatttgag ttttttaaac ttacggaggg ttcagtttca 960 gcaggtgctc ttccattact aaattggctg gatctaaatg gacagaagag agccatgaag 1020 agaacagcca agaagatgga ctccatagct gaaaaattgc ttgacgagca ccgacaaaaa 1080 aggctatcaa aggaaggtgt gaaaggcact catgatcata atgacttcat ggacgtatta 1140 ttgtccattc ttgatgctga tcaaggagat tattcccatc atccattcaa ctacagtcgc 1200 gaccatgtca taaaagccac cactctgtca atgatcctct cttccatgag catatctgtt 1260 agtctttcat gggcactctc tttattactc aacaaccgcc atgtcttaaa aaaggcgcaa 1320 gatgagttag acatgaacgt tggcaaggac agacaagttg aagagggaga catcaagaac 1380 ctggtctatc tccaagccat tgtcaaggaa acattccgta tgtatcctgc aaatccactt 1440 cttctacccc atgaagctat tgaagactgc aaaattggtg ggtttaacgt accagcaggc 1500 acacgtgtgg tagtcaacgc atggaaacta caacacgacc cacgcgtgtg gtcgaaccca 1560 tccgagttta agccagagag gtttctcaat gaccaagcag caaaggtagt agatgtgaga 1620 ggtcaaaatt ttgagtactt accatttggg tctggacgaa gagtgtgtcc aggaatctcc 1680 ttctccctcc aaaccattca catgtcacta gctcgcctag ttcaggcatt cgagctagga 1740 acgccctcga atgagcgcat cgatatgact gagggttccg gcttaaccat gcccaaaaca 1800 accccgcttc atgtccttct taacccacgt cttcctcttc cactctatga atg 1853 <210> 144 <211> 1599 <212> DNA <213> Eschscholzia californica <400> 144 atggagtttc attttcctct catggaacaa ttccaaccat ttttcatttt tgcccttctt 60 ctagcctcct tcatttttct ctacaaactc ttcaattttg gaaacagaat cactaaaaat 120 gggaaaccaa cagctccgga agcatcagga ggaagactaa ttatgggtca tcttcatcta 180 ttcaatggaa ctgagttaac tcaccggaca ctcggttcta tggctgataa atacggtcca 240 gctttcaata tccgattcgg ttcacataaa acacttgtag tgagtagttg gaaaattatt 300 aaagaatgtt ttactacaaa tgaccgattc ttttcgaatc ggcctggttc tttagcaatc 360 aaactcatgt tttatgatgc cgattcagtt gggtatgcac catatggatc gtactggaga 420 gaacttagaa aaatttcaac cttaaaactc ctttcgaacc atcgtttaga aaccctaaaa 480 catttgagaa cttctgaagt tgattcttgt tttaatcaac taatgaattc ttgggctgaa 540 aacaaaaaca ggggagactc tgattttgct ccggtgagga tggatgattg gtttggggat 600 ttgacgttta atgttgtagc aagaattgtt gccggaaaga agaatttcgc cggaggagca 660 gcgagaggtg atgccggagc tcagagatat aaatcagcca tggatgaagc ttttagactg 720 atgacaatat ttgcttattc agatgtgatt ccatctcttg ggtggttaga taaattgaga 780 ggactagttg gagatatgaa aagatgtggg tctgaaattg attcagtagt tgagagttgg 840 gtggatgaac atagattgaa aagaagggtt tctaagaaag gaggtgaatt agatcttgaa 900 caggatttca ttgatgtttg tttggatatc atggaacatt cttctttgcc tggtgatgat 960 cctgaaattg tcatcaaatc tacttgcttg gacatgatat tgggtggaag tgacacaaca 1020 acagtaaccc tgacatgggc cctatccttg cttttgaacc atccccaagt cctgaaaaga 1080 gccaaggaag aattagatag tcaagttgga aaggaaagac aagtagaaga ctctgacatt 1140 cccaaccttc ctctcatcca agctatcatc aaagaaacaa tgagactgta tccagccggc 1200 ccattgatcg aacgacgaac tatggaagat tgcgaggttg ctgggttcca cgtaccggct 1260 ggcacacgtc tattagtgaa tctatggaag atgcagagag acaaggaagt gtggagtgaa 1320 gaacctttag agtttagacc agagagattc ctaacgagca acacagaagt tgatctcaaa 1380 ggacaacatt atgaactgat accatttgga gcaggaagaa ggatatgccc tggtgtgtcc 1440 tttgcggtgc agttgatgca tttggtgctc gcacgtcttc ttcatgggtt cgaaatgaca 1500 acaccaatgg gtgagaaggt tgatatgaca gagagtgcag gattaattag tcacaaaatc 1560 acaccacttg aagttcttat caaaccactc agggtctaa 1599 <210> 145 <211> 1533 <212> DNA <213> Eschscholzia californica <400> 145 atggattcct tcttgcttgc ttattgggtt ccaatttcag ttgcttctat tattgctttt 60 gttttcctct acaatctctt ttcttcaaga actcttcaaa ataagaagat taggacagca 120 cccatggcaa cgggtgcttg gccgattctc ggtcatctcc atctctttgg ttctggtgaa 180 ttgcctcata aaatgctagc tgccatggct gacaagtatg gctcagcctt caggatgaag 240 tttggtaaac acacaacact agttgtgagt gatacccgta tcgttaaaga atgtttcact 300 accaacgata ccctcttctc taaccgtcct tccactaaag cttttcaact catgacttac 360 gataatgagt cggttgcctt tacaccttac ggtycttact ggcgtgagat tagaaagata 420 tccactctta aacttctatc caaccatcgt ctccaagcca tcaaggacgt aagagcctcg 480 gaggtaaacg tctgcttcaa aaccttatac gaccagtgta agaatccaag tggatcagct 540 cctattttga tcgatatgaa gaaatggttc gaagaggtgt cgaacaacgt ggtgatgagg 600 gtaattgtgg ggagacaaaa ctttgggtct aagattgtgc aaggtgagga ggaagctatc 660 cattacaaga aggtcatgga tgagctctta cgtctcgcta gcttgtctat gttctcggat 720 tttgctcctt tacttggttt cgtggacatc tttcaaggaa acttgagtgc catgaaacga 780 aacgccaaga aggtagatgc aatcctggag aactggttgg aagagcatcg caagaagaag 840 aactcagttg ctgaaagcca gcaagatttc atggatgtta tgttgtcgat tgttgaggag 900 agcaagttgt ctggtcacga tgctgatgcc gtaattaaag ctacttgtct agccatgatc 960 atgggcggaa cagataccac agcagtgagt ctaacatgga tcatttcttt attaatgaat 1020 aatcgtcacg ctttgaagaa agctcgagaa gagttagatg cactagtagg aaaggacaga 1080 caagttgaag attcggattt gaagaattta gtttacatga atgctattgt taaggaaaca 1140 atgagaatgt acccattagg tactcttctc gaacgtgaga ctaaggagga ttgtgagatc 1200 gacgggtttc atgtcaaagg tgggactagg ttgctagtga atgtgtggaa gttgcaacga 1260 gacccaaatg tatgggttga tccaacagaa tttagacccg aaagatttct aacggagaat 1320 gcagatatag atgttggagg tcagcatttt gagttgctac catttggagc aggacgaagg 1380 gtgtgccctg gggtgtcgtt tgcactacaa cttcatgcat ttagtacttg ctcgcctcat 1440 ccatggatac gatttgaata ctctaaacga agaaaatgtg gatctgacgg agagcccaga 1500 aggacatgtg aaccacaaag catcgcctct tga 1533 <210> 146 <211> 1605 <212> DNA <213> Glaucium flavum <400> 146 atggagtttc tctctctaca acttcaacca gtttccattt ttgctcttct tgttgcctcc 60 attttcctct acaatttctt aattcacgga aaaaaatcca acaacaagaa gaccacaaaa 120 ccaccagcac cagaagcatc aggtggatgg ccaattatgg gtcatctcca tctcttcaat 180 gaaaacgagt taactcaccg aacactcggt tccatggctg acaaatacgg tccagctttc 240 aacatccgat tcggttctca tcctacactc gttgttagca gttgggacat cgttaaagaa 300 tgtttcacaa caaacgaccg attcttctcg aatcggcctg gttcgttagc gattaaactc 360 atgttctacg atgccgattc agtcggttat gctccctatg gtgcttattg gagagacttg 420 agaaaaattt caactctgaa gcttctttcg aatcatcgat tggaaaccct aaaacatttg 480 agaacttctg aagttgaatc ctgttttaaa gaactttata atcagtggag gaacaacaaa 540 actggtggtg gtggagatgg ttttgctccg gtgaggatgg ataattggtt tggtgatttg 600 acgtttaatg ttgtggcgag aattgttgcc ggaaaaaaga attttgccgg tggtgcggcg 660 agtggtgatg ccggagctca gagatataag gaagctatgg atgaagcgtt taggttgatg 720 acgatttttg cgttttctga tgtggttccc gcgttggggt ggttggataa attgagaggt 780 ttagttggag gaatgaagcg ttgtggggcg gaaattgatt ctatagttgc ggggtgggtt 840 gatgaacatc ggttgaagag aagctctggc aagggaagtg atgctgatct tgaacaggat 900 tttattgatg tttgtttgga gattatggaa cattccacat tgcctggtga tgatcctgaa 960 gttgtcatca agtctacttg cctggacatg attttgggtg ggagtgacac cacaacagtg 1020 accctaacat gggccctttc cttactattg aacaatcccc atgtgttgaa aagggctagg 1080 gaggaattgg atacaaatgt tggaaaggat agacaagtag acgactcaga tatccctaat 1140 cttgtataca tccaagcgat catcaaagag actatgagat tatacccagc tggaccactg 1200 atcgagcgga ggacatcaga ggattgtgag gtaggtgggt tccatgtacc agctggtaca 1260 cgcttattgg taaacttatg gaagatgcaa agggacggga gtgtgtataa ggaggatcct 1320 ttggagttta gcccggagag attcctgact agcaacgcag atgtagattt aaagggacag 1380 aattatgaat tgataccatt tggggcaggt aggaggatat gtccaggtgt gtcatttgca 1440 gtccaattga tgcatttggt gctggctcgc ctcattcatg gcttcgaaat gaagacgcct 1500 gaaggtggaa aggttgacat gacagaaagt gcaggactaa ttagtcacaa ggtgacgcct 1560 ttggaagttc tgctcaaacc acgtctagca atccaacatt catag 1605 <210> 147 <211> 1686 <212> DNA <213> Glaucium flavum <400> 147 atgcatgcga aactgacctt gattaagaaa atggagttca ccatcatcaa ctctctagaa 60 atccaaccaa taatctccac tttcgctctt cttactttct ccattcttct ctacaaaatt 120 ctcttgaacc atggaagaga aaacaagaac aataaaccaa aaacatcatc atcatcatca 180 tcaataccag aagtagcagg tgcatggccg ataatgggtc atctccatct cttcaacgga 240 gacgagttaa tgcaccataa actcggttcc atggccgaaa aatatggtca agctttctat 300 atccgatttg gttctcataa agcggttgtg gttagcaatt gggagatggt taaaacatgt 360 ttcactacaa ataaccaaat cttcttaaat aggcctccca tgttagctat taacctcttg 420 tttttcccca ccgattcgct tagttacata ccgtatggtg accactggag agagttgaga 480 aaattttcaa accaaaaact tctttccaat caccgcatcg aaacccagaa aaatttgaga 540 aaattagaag ttgattactg ttttaaacag ctttgtaatc agtcttctaa gtattttata 600 attaacaaca tggacgacca agacagcaag tttgctctag tgaggatgga tacttggttt 660 gatgatgtga cattgaatgt tctggcaaga attattgccg ggaaaaagaa gtttatatcc 720 ggcggagcaa cgagtagtgg tgatgatgat aatgctgaag ctcggagata catggaagct 780 ttagatgaag gacttcgtct gatgacgagt ttcactttct ccgatgtgct tccgtggttg 840 gggtggttgg ataatttgag aggtttggct ggaaagatga agcgttgtgg tgcagaactt 900 gattcggttt ttgcggggtg ggttgaagaa catcgtgtga agagaggctc tagaaaggat 960 ggtgatgatg ctgatcttga acaggatttt attgatcttt gctgggagag tttggaacag 1020 gtgcctggaa atgatcctgc aaaaatcatc aagttaattt gcatggaaat gattttgaac 1080 gggagtggcg ccacggcagt gaccctaaca tggaccgtct ctttactatt gaacaacccc 1140 gacgtgttaa aaagggcaag ggaggagttg gatacacacg tcggaagtca tcgacaagtg 1200 gacgaatcag acatccctaa tcttgtttac atccaagcaa tcatcaaaga gggaatgaga 1260 ttgtatccac ctggaccatt ccttgagcga agtacaactg aagattttga gatagatggg 1320 gttcatgtac cagctgggac tcggttatgg gtgaacttat ggaagatgca ccgagacgag 1380 agcatgtatc aggaaccact cgagtttaaa ccagagaggt tcttgaatag caattcagat 1440 gtagatctaa agggacagag ttaccaattg ctaccatttg gggcaggtag gaggatatgt 1500 cctggcgtgt cgtttgcgtt gccgttgatg catctgacat tggctcgtct cattcatggc 1560 ttcgaaatga agttgcccgt aggtgttgaa aaggttgaca tgacagaaaa tggaggtata 1620 attaaccgga aggcgacaca tttggatgtg ctgctcaaac cgcgcctcat cgctcaacaa 1680 gcttaa 1686 <210> 148 <211> 1611 <212> DNA <213> Glaucium flavum <400> 148 atggatacac tttcaattca atggattgta ccttccatag ccacccttct tgccttagtt 60 ttcctctaca atctcatttt tacttccaaa aaaactacca agactaacaa cactaggaag 120 gcaccaatgg catccggcgc atggccggtt cttggccacc tccatttatt tggaaccggt 180 gagctacctc acaaaatgct tgcaacaatg gctgaaaact acggtaccgc cttcacaatg 240 aagttcggta accacactac actagtcgtt agcgacactc gcatcgtaaa agaatgtttc 300 actaccaacg acaccctttt cgcgaaccgt ccgtccacca aagcattcga tctcatgact 360 tatgccaatg actccgtagc cttcacacct tatagcccgt attggcgtga gcttcgaaag 420 atatcgactc ttaaacttct ctcgaaccat cgtctccaat cgattaaaga cgttcgcgtc 480 gcggaggtga acgtatgttt tagaggctta cacgttctat gcaagagtaa aatctatgga 540 gctccggttt tagttgacat gaaaaaatgg tttgaagagg tctcgaacaa tatagtcatg 600 agagtgatcg tggggaaaca aaattttggg tctaggattg tgcaaggaca agaggaggct 660 gtctattaca agagtgtcat ggacgagctt ttacgtctag ctagtgtatc cgttttatcg 720 gattttgcac cgttatttgg ttggttggat ttctttcaag gaaacataag tgctatgaaa 780 cgaaatggga agaaactaga tgtgatactt gagagatgga tggaggagca tagacaaaag 840 aagataagct catcgtcgtc gatagccgcg tccggcgccg gtgaagatga tgagcaagac 900 tttatggatg ttatgttgtc tatcattgag gagactaagt tgtccggtcg cgacgccgat 960 accgttatta aagctacttg cttggccatg atcatgggag ggactgacac tacagcggta 1020 agcctaacat ggatagtctc cttattgatg aacaatcgac atgtactgaa aaaggctcga 1080 gaagaattgg actcactagt tggaaaggat agacaagtag aagattcaga tttgaagaat 1140 tttgtataca tgaatgctat tgtcaaggaa acgatgcgat tgtatccgtt tggtgctttg 1200 ctcgaacgcg acaccaagga ggactgtgag gttggtgggt tccatgtcga agccggcacc 1260 cgtttactag taaacgtatg gaagttacaa cgagacccta atgtatggaa agatccatta 1320 gagtttcgac cagaaagatt tctggtcgag aacgtcgata tcgacgtcgg cggtcaacat 1380 ttcgaactat tgccgttcgg ggccggtaga agggtgtgcc ccggcgtgtc gttcgcactt 1440 cagttcatgc atttggtact agctcgtctc atacatggat atgaattgga aacactaaat 1500 ggtgaagatg tggatttaac tgagagcaca gaaggacatg ttaaccataa agcatcccct 1560 cttgatctcc tcatcacccc tcgcctcgac tctaaggtct acaattacta g 1611 <210> 149 <211> 1599 <212> DNA <213> Glaucium flavum <400> 149 atggatagta cccttgttct ccaatgtttt gtaggttcca tggcagctgt ttctgccttg 60 gttttcctat acaatctcat atttagctca tcgaaaacta ccaagggtaa ggttcttagg 120 aaggcaccta tggcagccgg ggcatggcca attcttggtc acctccattt gtttgggtca 180 ggtgagctgc ctcacaaaat gttatccaag atggctgaga agtatggccc tgcgtttaca 240 ttgaagttcg gtaagcacac gacactagtt gtgagtgaca cccgcgtcgt aaaagagtgt 300 ttcactacca atgatacact tttcgctaac cggccttcga ctaccgcatt tgatctcatg 360 acttatgcca atgactctgt agccttcaca ccttatagtc cttattggcg tgagcttaga 420 aagatatcta ctctcaagct tctctctaac caccgtctcc aatcgattaa ggaaatccgt 480 gtctcggagg tgaacgtatg ttttagggag ctatttgaga tgagcaagag caaaaccgat 540 ggagctgctc cggctttggt ggatatgaag aaatggttcg aagaggtgtc gaacaatata 600 gtcatgaggg taatcgttgg aagacaaaat tttgggtcta agattgtgca aggtgatgcg 660 gaggctgtca actacaagaa tgtcatggat gagctcctac gtctcgctag tttgtctatg 720 ttatcggatt tcgctccttt acttggttgg gtggatatgt tccaaggaaa caagaacgca 780 atgaaacgaa atgccaagaa agttgacacc atactagaag gctggttgga ggagcatagg 840 aagaagaaca agaagatgag ctcatcagaa aatgatgaac aagacttcat ggatgttatg 900 ctttcgatta ttgaagagac caagttatct ggccgtgacg ctgataccgt tattaaagct 960 actgtcttgg ccatgataat gggtggaaca gacaccacgg cggttagtct aacatggatt 1020 gtctccttat tgatgaacaa tcgtcatgta ttgaagaagg ctagagagga aatagacgcc 1080 attgttggga aggatagaca agtagaagat tcagatttga agaactttgt atacatgaac 1140 gctatcgtca aggaaacgat gcgattgtat ccacttggtg ctatgctcga acgcgacacc 1200 aaggaggact gtgaggttgg tgggttccaa gtacaagccg gcacacgatt actagtaaac 1260 gtatggaagt tacaacgaga cccaaatgtt tggagtgatc catcagagtt tagaccagag 1320 agatttttat cggagaacgc tgatatagac gtcggcggtc aacatttcga attactacca 1380 tttggtgcag gtagaagggt gtgtcccagg gtgtcgttcg cgctccaatt catgcatttg 1440 gtactggctc gtcttatcca tggatatgaa ttgggaactc aaaatgatga gcttgtggac 1500 ttaactgaga gcacagaagg tcatgttaac catatggctt cccctctaga tctcatcctc 1560 accccacgcc tcagcaaccc taagctctat gattattag 1599 <210> 150 <211> 1557 <212> DNA <213> Glaucium flavum <400> 150 atgttcttta cttcatcttc aaaaaccaca aacaaaaaca caagtaagaa accacccatg 60 gctcccggtg catggccaat actaggtcat cttcatttgt ttaaagaagg tgagttacct 120 caccacatgc ttaaatccat ggctgataaa tacggccccg ccttcctcat gaagttcggc 180 caacaccggt ccctcgtcgt cagcgactac cgcattgtta aagaatgttt cactaccaat 240 gacaccttat tttccaatag accgtctaca actgcctttg ctgtcatgac ttacgctacg 300 gactccgtcg cgttcaccga atatagtcct tattggcgtg agcttcgaaa gatatccact 360 cttaagctac tctcgaacaa ccgtctccag gcgataaaaa aactccgaga aagcgaagtg 420 aacgtctgtt tccgaggttt atatgattcg tggaggaaga ataagagtga acagaatggt 480 gctggtaata gtattgatgg tggtaacgaa cgagcacgtc cggttctcgt cgacatgaaa 540 aaatggttcg aagaggtgtc gaacaactta gtcatgaggg taattgttgg taaacgtaat 600 tttgggacta agattgttga aggggagaag gaggctgttg agtataagac cattatggat 660 gaactcttac gtcttgctag tttgtctttg ttatccgatt ttgcacctat acttcgtttg 720 tttgatcact ttcaaggaca tattcgtact atgaaacgaa atggcaagaa actagacgta 780 ctacttcaac ggtggttgga ggagcatcgg agaaagatga gcacgccgga ggaggagcaa 840 gatttcatgg atgttatgtt gtccattgtt gatgagagca agttgtctgg tcacgacgct 900 gatacggtta ttaaagctac ttgcctggcc atgataatgg gtgggacgga cacatcggcg 960 gtgagtctaa catggatagt ctccttattg atgaacaatc gtcatgcact agcaaaggct 1020 agagaagaat tggacaagca cgtaggtaaa gatagacaag tagaagaatc agatttgaag 1080 aacttggttt acttgcatgc aatcgttaaa gaaacaatgc gattatatcc attgggacct 1140 cttctcgaac gcgaaacgaa gcaggattgc gaggttggag gattcgatat cgccggaggc 1200 actcgtatac tagtaaacat atggatggta caaagagatc cggccgtctg gaacgacgcg 1260 acggagttta taccggagcg gtttctaacg gagaaatcgg atgtagatgt ttggggtggt 1320 agttttgagc ttataccatt tggagccggc cggagggtgt gccccggcgt gtcgtttgct 1380 ctacaattct tacatttagt attggctcgt cttatacatg gatatgaatt gaaaacgcca 1440 aatgatatgc cggtcgactt aacggagagt actgaagggc atgttaacca taaagcatca 1500 cctttggatc ttcttctcgt ccctcgcctt agcgacctga agctctatga ttactag 1557 <210> 151 <211> 1614 <212> DNA <213> Glaucium flavum <400> 151 atggattcca ttcttacaac cgttgttctc ctttccatac ttctctactt tctattctca 60 tggcagctaa acaaatactc tgctactaag aagaatacca aatcatcaaa acagctacca 120 ccagaaccgg ctggttcatg gcccgtcatt ggtcatctcc atcttctcgc caaaggatca 180 aacttgcccc atataaattt gggagccatg gctgacaagt atggaccaat cttcatgatc 240 cgtatcggct taaatccaac actggtggtt agtagttggg aggtagcaag agagatattc 300 acaaccaacg accaagtttt ctcctcccgt ccaattcaag tatccacgaa acacttgggt 360 ttcgacactg ctatgtacgg tttcgcacct tatggaccat actggcgtga aatcagcaaa 420 ttagtgaagc gtgaagttct atccaacact agactagagt tcctaaagcc tgtttggggc 480 tctgagatca acacatctat caaagaattg tatgatgtgt gtgtaatgaa aaacaaagaa 540 gaaggtggta ctggtccaat tattgtggaa atgaagcaat ggttctcaga tttagcatta 600 aacatgtcgg ttaagttggt agccggtaag agatattttg gtgcttctca actaggaaat 660 gaggaagctg cgaggtggca aaaggcactg agaaactgct ttaggttggt ggggctgttt 720 gttgtgtcgg acgcaatacc atttctaagg tggttggacg tgggtggtca cgaaaaagaa 780 atgaagaata ctgctaaaga gttggacgat ttgttggagg gatggctgga agaacataaa 840 atgaaaaaga agttatcatt aagtgaagtt gaagctgaga agaaggaaag ggatcgggtt 900 gacttcatgg acgttatgct gtccacactt gaacatgaaa aggcatctga ctatttcccg 960 gctgatacta tcaacaaggc tacttgcttg gctctaatcc ttggtgggac tgatactacc 1020 acagttgttt gggtttgggc cttggctcta ctagtgaata acccgaatgt gttaaagaag 1080 gcccaagatg agttggatgt ccatgtaggt aagaaaagac aagtggatga atcagacatc 1140 aaaaacctta catatctcca agccataatc aaggaatcaa tgcgtctcta cccagcagct 1200 acattaggta taagagaatc aacagaggat tgcactgtag ctggctacca catccctgca 1260 gggactagtt tgatagtgaa ttcttggaag attcaacatg acccacaagt atggactgac 1320 ccatttgaat ttcagccgga gagatttctc acaggccaca tggacgtcga tattagaggt 1380 cagagttgca aattcctccc ttttgggtcg ggtagaaggt catgtccagg gacatcactc 1440 gctcttcaaa tggtaacctt gacacttgct cgtttgatcc atgggttcga gttcaggact 1500 ccgtcagaag cacccactga tatgacagag agcgctggac taactaatgt taaggccacc 1560 ccacttgaag ttctagtctc accgcgcctt ccttcggagc tttatgtttg ttaa 1614 <210> 152 <211> 1614 <212> DNA <213> Glaucium flavum <400> 152 atggagttga tcaactctct tgaaatccaa ccaattacaa tatccatttt agctcttctt 60 actgtgtcca ttcttcttta caaaattatt tggaatcatg gaagcagaaa aaataataag 120 agcaacaaga acaacagaaa gacatcatca tcagctggag tagtagaaat accaggtgca 180 tggccaatca tcggtcatct ccatctcttc aatggaagcg agcaaatgtt ccataaactc 240 ggttccttgg ccgaccaata tggtccggcg ccattcttta ttcgatttgg ttcgcgtaaa 300 tatgtcgttg ttagcaattg ggagctcgtt aaaacatgtt tcaccgcaca aagccaaatc 360 tttgtaagcc ggccacccat gttagccatg aacatcttgt ttttcccgaa agattcgctt 420 agttatatac agcatggtga ccattggaga gaattgagaa aaatttcaag cacgaaacta 480 ctttccagtc accgcgtcga aacccaaaaa catttgatag catcagaagt tgattactgt 540 tttaaacagc tttataaact ttccaataat ggagagttta ccttggtgag gctgaatact 600 tggtgtgagg atatggcatt gaatgttcat gttagaatga ttgccgggat gaagaattat 660 gttgctgcac ctgggagtgg tgaatatggc ggtcaagctc ggagataccg gaaagcttta 720 gaagaagctc ttgatctact gaaccagttc actatcactg acgtggtgcc atggttgggg 780 tggttggatc attttaggga tgtggttgga aggatgaagc gttgtggtgc agaacttgat 840 tcgatttttg cgacgtgggt tgaagaacat cgtgtgaaga gagcctctgg aaagggaggt 900 gatgttgaac cggattttat tgatctttgc tgggagagta tggaacaatt gcctggcaat 960 gatcctgcaa ctgtcatcaa gttaatgtgt aaggaacata ttttcaacgg gagtggcacc 1020 tcgtcactga ccctagcatg gatcctttct ttaataatga ataatcccta cgtgataaaa 1080 aaggcaaggg aggaattgga aaaacacgtc ggaaatcatc gacaagtgga agaatcagat 1140 ctccctaatc tcttatacat ccaagcgatc atcaaagagg ggatgagatt gtacacacca 1200 ggaccattca ttgatcgaaa tacaactgaa gattatgaga taaatggtgt ccatatacca 1260 gctggtactt gcttatacgt aaacttatgg aagattcacc gagacccgaa tgtgtatgaa 1320 gatccactcg agtttaaacc agagaggttc ttgaagaaca attcagattt ggatctaaag 1380 ggacagaatt accaactcct accgttcggg gcaggtagga ggatatgtcc cggtgtgtcg 1440 ttagcgttgc cgttgatgta tctgacagtg tctcgactca ttcatggctt cgatatgaag 1500 ttgcccaaag gtgttgaaaa ggctgacatg acggcacatg gaggtgtaat taaccaaagg 1560 gcgtaccctt tggaggtgct gctcaaacca cgtctcacct ttcagcaagc ttaa 1614 <210> 153 <211> 1716 <212> DNA <213> Glaucium flavum <400> 153 atggatttac aaatcttctt ccacttccaa ggaattgtag gctcattagc tttactatca 60 ttcttctatt atctatggag acttttaact acgacgaaga cgagtatttg taacggtaca 120 acggctgcac cacctgaagt ttccggtggt tggccgatac tgggtcatct tttgcagcta 180 gtaggatcga aacagccgtt gttcaaggtt cttggagaca tggctgataa atatggacct 240 atttttgttg tccggtttgg gatgtaccca actcttgttg tgagcagttg ggagatggca 300 aaggagtgct tcagtaccaa cgatagagtc ctagctactc gtccaactag tgctgctagc 360 aagtacctta cttacaacta tgccatgttt gctttcacat tttatgggcc ttattggcgt 420 gagattcgta agatatcaac tatagaattg ttgtcccatc gacgtgttga gatgttcaag 480 catgttccgt tcatggaaat cgatacgtgt atagaacaac tatatcttct ttggatgcag 540 aaccagaacc agaaccagaa ccaaccgaac ggagatccgg ttcaggtaaa catgagtaaa 600 gtatttgaag aactaactat gaatgcggtg ttgaagttag tcgtcggaaa acgccttacc 660 gacgacaaag aaggcgaaaa gctgcacaag accattcaag aattctttaa actgttagag 720 gtatcagttg catcggatgt ttttccattc cttgggtggc tagatgtgga tggacaaaag 780 agaaaaatga aaagggtagc taaggagatg gacataatag ctgaaaaatg gcttgaagag 840 caccgccaaa agagaagtag taagttggag gaggaggagg aggaggagga ggacgacggc 900 ggcggcaaag gagacgcggc ggcggcagat aaggatttca tggacgtgtt gttatcctta 960 ttggaaggag atgaaggtga ttctgatcaa cccttcatga actatagtcg cgatacagtc 1020 atcaaagcca cctctctgaa tatactcgtg gctgctacag acactacatc acctacctta 1080 acatgggcta tctcattact actcaacaac ccccatgtct taagagaggc ccaaaatgaa 1140 ctagacatga aggtaggaag ggatagacaa gtcgaagagc aagacattga gaacctaatc 1200 tacctccaag caatcgtcaa agagacactc cgattgtacc cagctggtcc tctctctatc 1260 ccccatgagg ctattcaaga ttgtaaactt ggcggatatc atgtaagggc aggtacacgg 1320 ttgttgctga acatatggaa gctacatcgc gacccacgcg tgtggtcgaa cccacttgaa 1380 ttcaagccag agaggttttt aattctatcg gaggaggtgt gtggttgcag tcgtggaaca 1440 caaaattttg attttaaagg ccaatgtttt gagtacatac catttgggtc gggacgaagg 1500 atgtgcccag gatacaactt tggcatacaa atcattcaca tgacactagc acgcctactt 1560 cagtcattcg agatgcagcc tgcaaaagct aaatcgctaa atgatcaaga tgggcctgtc 1620 gacatgagag aaggttctgg cctaacactt ccaaagataa ccccattaaa agttctcctt 1680 acaccacgcc tctatggtca gctttataat cattag 1716 <210> 154 <211> 1605 <212> DNA <213> Hydrastis canadensis <400> 154 atggacttct ccatgctctt ccaatggcta ttggttacta tggctaccct tcttttcttg 60 aattctgttt acaatgtttg gtataaatct tcaaaaaaca ctagtattac tactacgact 120 agtaaaggaa agaaggcacc agtagctgct ggtgcacggc cgttcatggg tcaccttcat 180 atgttagtag ggggcaagca attgcctcac caggcacttg gaaagttggc cgatatatac 240 gggccagctt tcatcattca tattggccca aacccagaac ttgttgtaag tagttgggag 300 cttgcaaagg agtgtttcac tactaatgac aagtatttcg ctaaccgtcc tactaacaaa 360 gccatgaagt acttgacata cgacgaagca tccgttggct ttggacctta tgggccactc 420 tgggttgaaa tgcgaaaaat agctaaatct aatcttctct ctcaacaaag gctccaaatg 480 cataaacgtg tccgagtctt agaaatagac gcgtttttta aagaactcca tgagctatgg 540 tcgatgaaga aagaggatgg tccagtttct gtagacatga agcaatggtt tgaagaattg 600 acacttaatg ttatcaccag aatggtttct ggtaaacata aatatgcaac taaggcaaga 660 cggggtgata gtgaggcgaa gcaattcaaa agagtaatag gtgaggcagc acattttaca 720 ggaaatcttt tgctatcaga tatatttcca tctcttgggt tcttggataa catgcaaggt 780 cgtgtgaatt ccatgaagcg aacaggcaag gaacttgact ccatccttag ttcatgggtt 840 gaagagcatc gccaaaagaa actatcagga gagcagtcag aggatgaaga gaaagacttt 900 atcgatctta cgttgtccat gatggacgaa attcagctcc actcgactga ctcagagacc 960 ttcatcaaag ctatttgcgt gggtgtgatc ctaggtggga gcgacacaac atcagtggga 1020 ataacatgga tcctttctct actaatgaat catcgcgatg ttttgaagaa ggcccaagaa 1080 gaattggatc aacaagttgg aatggacaga aaagtagagg attcagatct gaataatttg 1140 gtatatctca gggcaatcgt aaaggaatca atgcgtttgt accaagttgg gccacttatt 1200 gaacgaaagg catcacaaga ttgcaccata ggcgggttcc atgtcaaagc aggaactcgc 1260 cttttggtga acctttccaa ggtacacaaa gacccaactg tgtggtcaga tcctttggaa 1320 tttcaaccgg agagatttct tacgacgcac tcaaacatgg accttaaggg acagcatttc 1380 gaactacttc cttttgggtc aggtagacga atgtgtccag ggtacttgtt tgccctcaat 1440 gaaatgtatt tggtgcttgc tcgtctcatt caaggctttg aactgggaac tcctatggat 1500 gcaaaggttg atatgacaga aacatcaagt gttaccaact acagagcaac accccttcaa 1560 gtattgctca ccccacgcct cagtcctaag ctatacgatt attaa 1605 <210> 155 <211> 1587 <212> DNA <213> Hydrastis canadensis <400> 155 atggcctttg ttgctcttat tgttgtttac aatatctggt ttaaatctgc agcaagcaga 60 aataagacca gctataataa caaaacaatg agcaagacac cagtagttga tggtgcgaag 120 cccatcattg gtcacctcca tttgttaatg ggaggtgacc tgcctcatca tgcgctcgga 180 agattagccg ataagtatgg gccaatcttc cttatgcaca ttggcgcatt cccagaactc 240 gttgtgagta gctcggaact cgcaaaagag tgtttcacta ccaatgataa atatattatc 300 aaccgtccta ccaataaggc catgacgtat ttgagttacg aacaagcatc tgttggtttt 360 gcaccttatg ggccactttg gattgagatg agaaagatct ctaaatccaa tcttctctct 420 aaccaaagga ttcacatgca gaagcaagta cgagtggcag aactggacgc gttctttaaa 480 gaactgtacc agctgtgtcg ttcaaacgat gaaaacaaca acaacagtac tagtcatggt 540 aaagttttgg tggagatgaa taaatggttt gaagaactga cactcaatgt agtgactaga 600 atgatatgtg gtaaacaaaa aatgggtact aaggcaaggc ttggggatag cgatgcgaag 660 cattacaaaa aaaccattga tgaggcagca cattttatgg gaaaccttgt gatctcagac 720 gtagttccat gtctaggaat gttggacaat ttactaggtc atgtgagcgc catgaagcgc 780 acaggcaagg aacttgacac aatctttggt tcatgggttg aagaacatca acaaaagatc 840 agactctctg gttataaaga tgatgctgaa gaggaggagc atgactttat tgatcttaca 900 ttgtccatga tgaagggatc aactgacctc catggtcttg accctgcaac tttcatcaaa 960 tctatatgtg tgggtatgat ccttggcggc acggacacga catcggtggc cttaacatgg 1020 atcctctctc tactactaaa taatcgccat attttgaaga aggcccaaga agaattggac 1080 catcaagtag gaaaagagag aaaggttgaa gattcagatc tcaacaatct ggtctttata 1140 ggagccattg tgaaagaatc aatgcgttta tacccagttg ggcctcttat tgaacgtgag 1200 gcaatagaag attgtcagat tggtggtgtc catgttaaag ccggcacgcg attactaata 1260 aacatttgga aggttcaaca agatcctaaa atttggccta atccttcaga gtttcgacca 1320 gaaaggtttc tggattctaa catggatgtt aagggccagc atttcgaact cattcctttt 1380 gggtccggta ggcgaatgtg tccaggaatg tcgttcgcca ttaatgaatt gaatttggtg 1440 cttgctcgtc ttcttcaagg cttcgagttg gaaactccaa tgaatgcaaa ggtagacatg 1500 actgaaactt caagcgttac gaattataag ggaacccctc tccaagtact tctcacccca 1560 cgtctcagtt ctaagttata tatgtaa 1587 <210> 156 <211> 1596 <212> DNA <213> Hydrastis canadensis <400> 156 atggagtatt cccatcttct cccatggcta gcaacttcaa tagctgccat ttttgctttc 60 atttttctct tttatgtccg gaggaggaaa aacaacaaag cctttattca taatcatgcc 120 aaaaaagcac ctgtggtacc gggtgctttg ccgtttcttg gtcacctccg cctatttact 180 ggaccgattt tacctcacaa agcgcttgga gcacttgcgg ataagtacgg tccggctttc 240 actatctatc tgggaagtca tcaaacactt gtggtcagtg gtcgagagtt ggtaaaagaa 300 tgcttcacta ctaatgatag attgttctct aatagaccca gaagtaaggc tgtcaagtac 360 ttgacttatg atgaagcctc agttggtttt gcaccttatg gcccactttg ggttgagatg 420 cgaaaggttt caaaactcaa ttttctttct aatcaacgga tccagatgca gaaacaggca 480 ctagcctcag aattgaattt ttgcttcaag gatgtgtatc aattgtggtt gaaaaataag 540 gaactccccg ttatggttga tatgaccaaa tggtttgaag aaatgacgtt aaatgtgata 600 acaagattga tttgtgggaa acaaaactat ggatctaagg ctaacagtgg tgaaagtgag 660 gccaaaaggt ataaacaagt tgtagaaaag gcagcacatc ttacatcaac tgttgtgatg 720 tcagatgtgt ttccttttct tgaatggttt gataagtttc aaggacagga gaaagtcatg 780 aaaaaagtag ctaatgaatt tgattcaatc cttggttcat gggtcgacga acatcgtcga 840 aagagacttc ttcgaggcaa caatgaggag gaggaggaag agcaggactt cattgatctt 900 tccttggcca tgatggagga aactcagctt catggtgttg atcctgatac cttcatcaag 960 tcaatgtgtt tgggcatgat ccttggagga ggtgacacta cgccggtggc cctaacatgg 1020 gccctttcgc tactattgaa taacccagat attatgaaga aagcccaaga agaaatagac 1080 caagtcatag gaaaagagag aaaattagac gggtcggata taagtaatct ggtctacctc 1140 caagccgttg tgaaagaatc aatgcggtta taccaagttg ggccattgat tgaacgcgaa 1200 acaactgagg attgtaagat tggtgacttc catgttgaag caggcacgcg tttactagta 1260 aacatttgga aagtacaaca agatccatgt gtgtggtcga accctacgga gtttcaaccg 1320 gagagatttc tttctagtaa gtcagatatg gatcttaagg gtcagcattt tgaactaatt 1380 ccttttgggt caggtagacg aatgtgtcca gcagttgctt cagctctcca aatgatgcat 1440 ttggtgcttg caaaactcat tcatggcttt gaattaggaa ctcccatgaa tgcaaaaatc 1500 gacatgacag aaacttcaag tataaccaac aacatggcaa ctcatcttca agtgctactc 1560 aacccacgtt taaatgctaa tctttatgat ttttag 1596 <210> 157 <211> 1632 <212> DNA <213> Hydrastis canadensis <400> 157 atggcggatt tcaccatgct cctccaatgg ttattgctta ccatggctac ccttcttttc 60 ttgaatgctg tttacaaaag tattaaatct tcaaaaaaca ctattaatga taccagtttt 120 aaaaaaggaa agaaggcacc agtagctgct ggtgcaagac cctttattgg tcacctccat 180 atgttagtag gaggcaaaca actacctcat caagctctcg ggaagttggc cgataagtat 240 gggcctgcct tcatcatcaa tattggtcca aacccagagc ttgtcgtaag tagttgggag 300 cttgcaaagg agtgtttcac tataaacgat cggtgtttca ctgatcgtcc cagtaacaaa 360 gccatgaagt acttgaccta cgacgaagcc tctgttgggt ttgcacctta tggccctctc 420 tgggttgaaa tgcgcaagat tgctaaatcc aattgcgcat ttcaacaaag gctccaagta 480 cagaaacgtg tacgtgtctt agaaattgac ttatttttta aagagctcca cgagctatgg 540 tcccacgctg gtgctggtgc taccggtact agtgtaattc ctctttccat agacatgaag 600 aaatggttcg aggaattgac actaaacgtg atcaccagga tggtgtctgg aaaacataat 660 tatgcaacta aggcaagaaa gggtgatact gaggcgaagc gattcaaaag agtgataagt 720 gaggcagcac attttacagg aagacttctg ctctcagata tatttccatc acttgggttc 780 ttggataaca tgcaaggtcg tgtgaactcc atgaagcgaa caggcaagga acttgattcg 840 gtccttagtt cctgggtgga agaacatcgc caaaagagag tctctggaaa taaagagcag 900 tcacctgagg atgatgatgt ggaacaagat tttatcgatc tcacgttgtc catgatggag 960 gaaattcagc ttcactggac tgacgctgac accttcatca aagctatctg cgtgggtgtg 1020 atccttgggg ggagcgacac gacatccgtg ggcataacat ggattctttc tctgctaatg 1080 aatcatcccg atgttttgaa aaaggccaga gaagaattgg accaacaggt aggactggaa 1140 agaaaagtgg acgattcaga tctcaataat ctggcctatc tcagagccat tgtaaaggaa 1200 tcaatgcgtt tgtaccaagt tggcccactg attgaacgta aggcaaaaca agattgcaac 1260 ataggtgggt tccatctcaa agcaggcaca cgcctacttg tgaacctttc caaggtgcac 1320 aaagacccga ctgtgtggtc tgatcctaat gagtttcgcc ccgagagatt tttcaccacc 1380 cacaccaaca tagatatcaa ggggcagaat tttgaactca ttccttttgg gtctggcaga 1440 cgtatgtgcc cagggtactt atttgctctt agtgaaatat atttggtgct tgctcgtctc 1500 attcatggct tcgaattgag aactcccaat gatgccaagc ttgatatgac agaaacttcc 1560 agtgttacca attacagggc aacacctctc caagtactac tcactccgcg cctcagttct 1620 aagctctact ga 1632 <210> 158 <211> 1599 <212> DNA <213> Hydrastis canadensis <400> 158 atggactact tttccatgcc ttaccaatgg ttactaactt ccttagctac tcttattgtc 60 tttgttttta tctggtctat tactgctgga aaaacaagta ctacttctaa caaagcgaaa 120 aaggcaccgc caatagcggc tggtgcatgg cccattatcg gtcacctcca tttgataatg 180 gggggtgaac tccctcatcg attgctttcg aatttagccg ataaatatgg cccaatcttc 240 atgctcaact atggttcaca acctggatta gttgtgagta gcttgaagct tgctaaggaa 300 tgttttgttc ataatgatag agctgttttt aaacgtccta atagcaaagc catgaagtat 360 ttcacttatg atcaagcttc atttggtttt gcaccttatg gaccactttg ggttgaaatg 420 agaaaagtgt ctaaatcaaa tcttctttct aaccaaaggc ttcaattaca aaggaatcaa 480 cgtgcctcag aagttgatgc ttttataaaa gagctttacc aactatgtaa gaagtccaac 540 aatggtactc ttatggtaga aatgaataaa tggtttgaag aattgacact taacgttgtg 600 actagaatgg tttgtgggaa aaaaaacatt ggggctaaag caaggcatgg tggtgataat 660 gaggcaaaat attataagaa agttatagat gaagcaacaa tatttacagc taaattggtg 720 gcttcagatt tttttccttc tcttggatgg gtagattatt tgcatggtga tgagagtgct 780 ataaagcaga cagccaaaga acttgattcc atcattggtt cctgggtgga agagcatcgt 840 caaaagagac tactctcgct caataaagac gactacgcag agcaggattt tatcgatatt 900 acgttgtcca tgatcgacca aactcagcac cagggtattg atgctgatac cttcgtcaag 960 tctatgtgtg tgggtatgat ctttggtggg agtgacagca catcggtggc cctaaattgg 1020 gctctttcaa tactaatgaa taatcgacat gttttaaaga gggcccgaga agaaatcgac 1080 acacttgtag ggaaggacag aaaagtgaac gatgtggatg ttactaaatt ggtttatctc 1140 aaagccattg taaaagaatc aatgcgttta tgcctagttg gaccgcttct tgaacgtgtg 1200 accgtagaag attgcgagat aggtggtttt tatgtcaaag caggctcgcg catagtggta 1260 aacatatgga agttgcaaca tgacccagac ctgtggtctg atgatgttat ggaatttcga 1320 ccagagagat ttctcacaac caactcaaat gtggaactta ggggtcaaca ttttgaactc 1380 attccttttg ggtctggtag tcgaatgtgc ccaggtgtaa cattcgcact cgaactcatg 1440 cacttgacac ttgctcgtct cattcatgga tttgaacttg gtacccctat ggatgcaaag 1500 gttgacatga cagaaacttc aagtgttacc aattacaagg ccacacccct tgaaatcctt 1560 ctcaccccac gccttcatcc taagctttat gatttttag 1599 <210> 159 <211> 1605 <212> DNA <213> Hydrastis canadensis <400> 159 atgtttatat cgatcactca acaatccatg gattcatttc accaatatct agcaactata 60 atttttggtg tcttttcctt tgtattattc ttgatctatt atctaccatt gaagagaagt 120 agaagtgata aaaagagagc tgctcccaaa ccggtcgggg catggcctat cataggtcac 180 ttgcctatgt tatcccggca ccaaccaccg cacataacgt tgggaaatat tgctgataag 240 tacggaccag ctttcactct tcagcttggc gtgcatcgag cattggtggt gagtagttca 300 gaggttgcca aggagtgttt cactaccaat gacaaggcct tagcttcccg cccaagctct 360 gtggctttaa agctaatggg ttataacaat gctatgtttg gcttcggccc ttatggttca 420 tattggcgac aaatgcgcaa aatagttgta cttgagcttc tctcaaacca tcggctccaa 480 ttgctaaaac acgttcgtat atctgaggtt agcacatctt taaaagaatt gtaccaggtt 540 tgggcatcat gtactaataa gaacgacaaa ggacaagttt tggtggatat gcagcaatgg 600 tttggtgact tgacactaaa cgtctccgtt aggatgattg ctgggaagcg atactttggt 660 gccagtgcag catgcgacga agatgaagcg aggaggtttc agaaggcgat taaagatttc 720 ctccatttag taggcttgtt tgtggtgtca gacgcgcttc cctttctaga atggctagat 780 attcagggtc atcagaaagc catgaagaga actttcaaag aattagatag aatacttggg 840 aaatgggtgg aagaacatcg acgaaataaa ttagacggtg gaacgaatgt gggacgagac 900 ttcatggatg tgatgtcttc gatacttgac gatgcaaata tctctgatta tgatgctgat 960 accattaaca aggctacttg cctgagtctg atcttgggtg ggactgacac cacaatggtc 1020 actctaacat gggctttatc cttattaatg aacaatcaac acatattgaa gaaggcccaa 1080 gatgaaattg acatctatgt tgggaaagat aaaaacgttg atgaatcaga tatagagaaa 1140 ctggtgtacc tccaagccat tgttaaggaa acattgcgtt tgtacccagc cggtccacta 1200 tctgggccac atgaggccat agaggactgc actgtagctg gttatcatgt accaagaggc 1260 acacgcttga taacaaatct ctggaagatt caacgagatc cacggatatg gtcgagccct 1320 tgtgagttcc aaccagagag atttctcacc actcaagcaa atgtggatgt taggggccag 1380 cattttgagt ttcttccatt cgggtctgga agacgttcgt gccctgcgac ctcatttgca 1440 cttcaagttg tgcacttagc attagcacgt gtattacaag gcttcgagtt tgaaactcaa 1500 tcaaatgcac ctgtggatat gaccgagagc gcgggactta caaatgtcaa agccaccccg 1560 cttgaagttc taatcacccc gcgcctccct ttgaatctat attaa 1605 <210> 160 <211> 1590 <212> DNA <213> Hydrastis canadensis <400> 160 atggagtcgc ttcaccaata tctagcagca gtcatgagtt gtatctttgc cttgttactc 60 ttcctctatt atttcccatg gaagatcaga agaagtagct ctgataataa catgagaagt 120 gcacctgaag cagctggtgc atggcctatc ataggtcact tgcccatgct gggagggcac 180 caactacccc acataacgtt gggaaacttg gccgataggt acggaccagc cttcactatc 240 cggcttggtg tatgccgggc actggtggta agtagttcag aggtcgcaaa ggagtgtttc 300 actaccaatg acaaggcctt tgccacccgc ccaagctctg tggccgtaaa gctaatgggc 360 tataactatg cactctttgg cttagcccct tatacttctt attggcgtga agtgcgcaaa 420 atagtaatac tggagcttct ctccaatcgt cggctagagt tgctaaaaca tgttcggatc 480 tctgaggtca acacatctat aaaagaattg ttccaagttt gggcatcatc aaataataag 540 aatgagaaag gacaagtttt ggtagagatg cagcgatggt ttggtgactt gacaataaac 600 gtagctgtta ggatggttgc tgggaaacga tacttcggtg ctagtgttaa tacgtgcgat 660 gatgaggagg aggcgaggag gtttcaaaag gcgattaaaa atttctttca tcttgtaggg 720 ttgtatgtgt tatcggattc tcttccattt ttggagtggt tggactttga gggtcatcaa 780 aaggctatga aaagaacttt caaagatgtg gactgtatac ttcaaagatg gttggaagaa 840 catcgacgag ataaagagaa tggtgcaatg aaggaggagc gagacttcat ggatgtgatg 900 ttgtcgatcc ttaaagatgc aaatctcttt ggttacgaag ctgatactgt taacaaggcc 960 actagtctga atataatatt gggtgcaaca gacactacaa tggttactct aacatgggcc 1020 ctatcgttat tattgaacaa tcgacacata ttaaagaagg ctgaagatga aattgacatc 1080 catgttggga aagataaagc tgttgatgaa tcagatatag agaaactagt atatctccaa 1140 gccattgtta aggaaacatt acgtctgtac ccagttgctc cactgttagc agcacatgaa 1200 gccatagaag actgcattgt agctggttat catgtaccaa gaggcacacg cttgatacca 1260 aatatttgga agattcaacg agatccacgc atatggtcta gtccttgtga tttccaacca 1320 gagagattcc tcacaaccca agcaaatgtg gacgttaggg gtcagcattt cgagttgttt 1380 ccttttgggt ctgggagaag aatgtgccca gggatctcat ttgggcttca agtagtgcac 1440 ttggcactag cgcgtgtatt acaaggcttc gagtttgata ctccatcaaa taaagctata 1500 gatatgactg aaagcgcagg acttaccaac ctcaaggcca cccctcttca agttctaatc 1560 accccgtgcc tccctttgaa tctgtattaa 1590 <210> 161 <211> 1593 <212> DNA <213> Hydrastis canadensis <400> 161 atggattcaa ttttactacc aactccatta atggtcagtc tctttgcctt actattatgt 60 ctttactatt taatcatatc gaggccaagg tctagtcata atactagtac taaggaggca 120 cccgaagccg ctggagcatt gcctattatc gggcacctcc atttgttagg tgggcgaata 180 ttgcctcaca taacattagg agccatggct gataaacatg gaccagcctt catgattcgc 240 attggtgtac atagagcatt ggtggtaagt tcttcagaag ttgcaaagga gtgtttcaca 300 accaatgaca aggctttcgc ttctcgtcct aagcatacag cagcggaact catgggttat 360 aattatgcca tgtttggatt tgcaccttac ggtccttttt ggagcgaaat gcgcaagata 420 atcatggctg aactcctctc caatcgtcgg cttgagttgc ttaaatacat ccgggatttc 480 gaattaaaag catctatcaa agaactatac atgacatggg agaaccacag tgttactaat 540 aaaggtcagg tagtagtaga aatgaagaaa tggtttggag acttaactct aaatgtgatt 600 ctaaggatga ttgctgggaa aagatacttt ggttcaaatt ctacttgtga tgaaagtgaa 660 gcaaagatat gtcaaaaggg tatgagagat ttctttaggt tattgggaga gttccttgtt 720 gaggatgcaa ttccttattt ggggtggttg gacttgcaag gttttaaaaa agagatgaag 780 aatacagcta aagaacttga tattcttctt caaggatggt tggaagagca caaaaaaaag 840 agggagttct ctaaggatgt taaggaagag caagacttca tggacgtgat gatgaccata 900 cttgaagatg caaacttctc tgattttgat gctgatacta tcaataaggc tacgtgtttg 960 actataatct caggaggaag cgacactaca atgcttactc taacatgggc cctatcctta 1020 ctactgaaca atcaacatgt gttgaagaag gcccaagatg agttggacac ccacgtcggt 1080 agggatagac gcgtggacga atcagacatc aagaacctcg tctaccttaa cgccatagtg 1140 aaggaaacat tgcgcttata cccagctagt ccactactgg gtattcgggt gtccacagaa 1200 gactgcactg tagctgggta ccatgtccca tcagggaccc gtttaatggt aaatgcttgg 1260 aagattcaac gagacccgtt agtgtggtcc gacccatttg aatttcgccc agaaagattt 1320 ctcacaaccc atgtaaatgt ggatgttaag ggtcaaaatt ttaatttaat cccatttgca 1380 tcaggtagac gtgtgtgccc aggagttgca tttgcccttc aaatgctgcc actagttcta 1440 gctcatttgc tacatgggtt caaactcatg actcagttgg gtggacctgt cgatatgact 1500 gaatccactg ggttaactaa tatcaaagca actccattgg aagtagttat cagcccacgt 1560 cttcgtccag aactgtatga aatgtacaca tag 1593 <210> 162 <211> 1578 <212> DNA <213> Hydrastis canadensis <400> 162 atggagtcgc ttcaccaata tctagcaaca attttgagtt gtatctttgc ctttttactc 60 ttcctctatt atttcccatg gaagggaaga agaagctttg ataataactt aagaactgca 120 cctgaagcag ctggcgcatg gcctatcata ggccacttgc ctatgttaaa ccaacgtgat 180 ttaccccacg taacgttggg aaacttggcg gataggtacg ggccagcctt cactatccgg 240 cttggtgtac gccgagcact ggtggtgagt agttcagagg tcgcaaagga gtgtttcact 300 accaatgaca aggcctttgc cacccgccca agctctgtgg ctgtaaagct aatggcctat 360 aactatgcag tctttggctt tggcccttat ggttcttatt ggcgtgaaat gcgcaaaata 420 gtaatacttg agcttctctc taatcgtcgg ctagagttgc taaaacatgt tcggatctct 480 gaggtcaaca catctataaa agaattgtac caagtttggg catcaaataa taagaatgag 540 aaaggacaag ttttggtgga gatgcaacga tggtttggtg acttgacaat gaacgtagtt 600 gttaggatgg ttgctgggaa acgatacttc ggtgctagtg ttacgtgcga tgaggaggaa 660 gcgagaaggt ttcaaaaggc gattagagat tttagtcatc ttgctggttt gtatgtgttg 720 tcggatgctc ttccaaatat agagtggttg gactttgagg gtcatcacaa ggccatgaaa 780 aaaactttca aagatttgga ctgtatactt cagagatggt tggaagaaca tcgacgagat 840 agagagaatg gtgcaacgaa gggagagcga gacttcatgg atgtgatgtt gtcgattctt 900 aaagatgcaa atccttttga tttcgaggct gataccgtta acaaggctac tagcctgaat 960 atggttttgg gcggaacaga gactacaact gttactctaa catgggccct atcgttacta 1020 ttaaacaacc aacacatatt gaagaaggcg caagatgaaa ttaacatccg tgttgggaaa 1080 gacaaacctg ttgatgaatc agatatagag aaattggtgt acctccaagc cattgtcaag 1140 gaaacattgc ggttgtaccc tgtccttcca ttatccgtac cccacgaggc catagaagac 1200 tgcaccattg ctggttatca tgtaccaaga ggcacacgct tgataacaaa tctatggaag 1260 attcaacgag atccacacat atggtctagt ccttgtgagt tccaaccaga gagattcctc 1320 acaacccaag caaatgtgga cgttaggggt cagcatttcg agttgtttcc ttttgggtct 1380 gggagaagaa tgtgcccagg gatctcattt gggcttcaag tagtgcactt ggcactagcg 1440 cgtgtattac aaggcttcga gtttgaaact ccatcaaatg tacctatgga tatgactgaa 1500 agcgcaggac ttacgaattt caaaacaacc cctcttgaag ttctaatcac cccatgcctc 1560 cctttggacc aatattaa 1578 <210> 163 <211> 1578 <212> DNA <213> Hydrastis canadensis <400> 163 atggattctc ttctccagtt acaaataatt ggagcacttg ctgccttaat attcacttac 60 aaactattga aagtgatatg tagaagtccc atgactgatg gcatggaagc cccggaacct 120 ccgggagctt ggccgattat cggacacctc catctgctcg gtggccaaga cccaatagcc 180 cgtacacttg gagtcatgac tgataaatat gggccaattt taaaactccg gcttggtgta 240 catactgggc ttgttgttag taattgggaa ctggcaaagg agtgtttcac tacaaatgac 300 agagttttag cttcacgtcc tatgggtgca gcaggaaagt acttgggcta taattatgcg 360 atatttgggc tcgcgccgca tggaccatat tggagtgaag tacgtaagat agtattacgt 420 gaacttctct ctaatcagag tttagagaag cttaaacatg ttcgaatctc cgagattaat 480 acatgcttaa agaatttatt ttcattgaac aatgggaata ctccaataaa agttgacatg 540 aagcaatggt ttgaacgccc aatgttcaat gtggtcacta tgatgattgc agggaaacga 600 tattttagta tggagaatga caatgaggca atgaatttca ggaaagttgc cactgaattt 660 atgtacttaa ctggggtgtt tgtagtgtcg gatgcccttc catatctgga gtggttggat 720 ttgcaaggac atgtgagcgc catgaaacgc acggccaagg aattggacat ccatgttggg 780 aagtggcttg aggagcatcg tcgtgctaag cttctgggtg aaactaaaaa tgaagatgat 840 tttgttgatg tgctgctaac catcttgcca gaggacttga aggataatca aacgtatata 900 catgaccgcg ataccatcat caaggcaact gcactggctc tttttttagc tgcctcagat 960 actacagcta tcaccttgac atgggctcta tcgttgatac tcaacaatcc ggacgtcttg 1020 aaaagagcac aagatgagtt ggacaaacac gtcggtaaag aaaaactcgt caaagaatca 1080 gacatcataa atctagtgta cctccaagct atcatcaagg aaaccttacg cctgtatcca 1140 gctgcacccc tcttattgcc acatgaagcc atggaagatt gtacggtagg tggctatcat 1200 gttccaaaag ggactcgaat ttttgttaac atatggaagt tgcagcgtga tccgcgtgta 1260 tggtttgatc ctaacgagtt tcgaccagaa cgattcttaa ccactcatgc aaatgttgat 1320 tttaagggac agcattttga gtacatacct ttcagctctg gtagaagggt atgcccaggg 1380 atcacattta gtacccaaat catgcactta acacttgccc atctacttca tgaattcaac 1440 atagtgaccc ctacgaaatc aaatgcaggg gttgacatga ctgagagctt aggcatcaca 1500 atgcctaaag caactccact agaagtactt ctcactccac gtcttccatc taatctgtac 1560 aatcaataca gagactaa 1578 <210> 164 <211> 1566 <212> DNA <213> Jeffersonia diphylla <400> 164 atggattcac ttcaactcat agctagtgtt gtgtgtggat tgtttgcctt ccttcgtatc 60 tactacttac taaagaagtt gtctagtaac aaggaggcac cccaactcgg tggagcatgg 120 ccgattatcg gccacttaca tctattaggc cgagttgagc tcccccacat attttttagt 180 gccatggcag acaagtacgg tccagccttc atgattcagc ttggtatgcg acgagcacta 240 gtagtgagca gtgcggagat tgccaaagaa tgtttaacta caaatgataa ggccttagcc 300 actcgtccaa gttcaatagc agctgagatt atgggttatg actatgctat gttcgggtta 360 ggtccatacg gggactattg gcgtgaagtg cgcaaaatag tggtcttgga gtttctctct 420 aatcgtcggc ttgagttgct aaaacatgtt agggtgtctg aaatatccct gtctctgaaa 480 gagttgtacc aatcctggga aaaacaaaac aaagctgatg aaccagtttt agttcatctg 540 gaccaatggt ttggcgatat gacgctcaat ctttcagtta gaatggttgc tgggaaaagg 600 tactttggtg caactgctgc ttgtggtgaa gatgagtcta ggaaagttca aaaggcaaca 660 agggaattcg ataggctttt gggtctgttt gtagtgtcgg attatcttcc ttttctgcgg 720 tggctggact tggaaggtca ccagaagttg atgaagagca taggtagaga acttgattct 780 attctgcagg gatggttgga tgagcataaa actaggagga actctggtgg aggagctaag 840 ggagatcagg atttcataga cgtaatgctt tccgtccttg aagatggaaa cctttccaat 900 tacgatgctg ataagctcaa caaggctaca tcacttacga tgattgtagc tggaagcgaa 960 actacaatgg tcactctaac gtgggctgtc tgcttgttgc taaacaatcc ggaagtgtta 1020 aacaaagctc aagatgagtt ggacatgcac attggcaagg acagacaagt ggaagaatca 1080 gacatcaaga acctggcata cctccaagct gttatcaaag aaacgatgcg tttgtaccca 1140 gttgctccac tattaattcc ccatgaagcc atggaagact gcaccatagc tgggtatcat 1200 gttcgagcag gcacacgagt tatactaaat ggttggaagc ttcaacgaga cccatttatt 1260 tggtctgacc cttgtgagtt ccaacctgaa agatttctca acaacgatgt ggatgtgaga 1320 ggtcggcatt ttgagttatt gccatttggg tcaggtagac gtgcatgccc tgggatttca 1380 ttggccctta atgtggtgtc tttgacattg gctcgtttgt tacatgagtt caagttttcg 1440 aatccttcag aaggcaaggt tgatatgtct gagagtgcag gggtggttgt tgccaaggca 1500 acacctctgg aagtacttat cattccacgc ctaccttcaa agttttacga gtactataag 1560 ctatag 1566 <210> 165 <211> 1557 <212> DNA <213> Jeffersonia diphylla <400> 165 atgttttctc ttcagttgct atcaactcca ttgtttggct tctttaccct gctggccatt 60 tactatctct tatggactaa gagtaacaaa accaaggaag cacctcaacc tgctggagca 120 tggccaataa tcggccacct ccatctacta gccaggagtg aactccccca tataactttg 180 ggagccatgg ccgataagta cggtccagct ttcatgatcc gccttggtac acgccaagca 240 attgtagtga gtaattcaga gattgcaaag gagtgcttga ccatcaaaga taggatcttc 300 gctactcgtc caagcttagt agcatttaag ctcatgggct acaactctgc catggtcggg 360 ttaagccctt atggacctta ttggcgggag ttgcgtaaga taatcgcgtt aaaagtcctc 420 tcacaacgta ggctggagtc tctaaaggat gtgtgggact cagaaatatc ctcatcctta 480 aaccacttgc accaaatatg gtcacaccaa accgaaccca atggaaagat tttagttgaa 540 atggatcaat ggtttggtga tctaacacta aatgtggcag ttaagatggt tgcagggaag 600 agattcttta gtgctgatgc tgcttgtgat gaaaatgagt ctagaaggtg tcaaaaggca 660 ataagagagt tctttaggct tttgggtcaa tttgtagtat cagattctct tccatttcta 720 gggtggttgg acttggaagg ttatcagaaa gagatgaaga gtacagccaa ggaagttgat 780 tcaatacttc agaaatggtt ggatgaacat aaacagaaaa gacaatctgc aggagctgat 840 agagatcagg atttcatgga cgtgatgctt tctctcctag aagatgcaag cctctcccaa 900 tatgacactg ataccatcaa caaggctacc tgcttttcaa tgatcgtagg tggaagtgat 960 actacgaaga tcacactgac atgggctctc accttactac taaataatcc agatgtaatg 1020 aaaaagagcc aagacgagtt ggatatccag gttggaaaag ataggcatgt cgaagaatca 1080 gatatcaaga atctagtata cctggaagct atagtgaagg aaactttgcg tttgtatcca 1140 gctgctccac tattagctcc ccacgaggcc acagaagatt gtattgtagc tgggtataat 1200 gttcgcgcag gcacacgctt aatagtgaac gcttggaaga ttcaacgaga ccccttgatt 1260 tggccgaacc cttctgagtt tctaccagag agatatctta acaaagatgt cgacgttaaa 1320 ggacaacatt ttgaattaat cccatttggg tcaggtagac gtgcttgccc tggaatcaca 1380 tttggacttc aagtggtatc cttgaccttg gctcgtctcc tccatgagtt tgagatttcc 1440 attgcatcag gaattaaagt ggatatgacg gagagcggag gtctagttac tgccaaggcc 1500 acccctttga aagcacttat cgccccacgc tttctttatt ctcatcatca catttag 1557 <210> 166 <211> 1578 <212> DNA <213> Mahonia aquifolium <400> 166 atgaattcac ttcagttcct agcaactcca ttgcttactc tcattgcatt actatgtgtt 60 tactgcatag tatggagaaa atcattctct agaaacggaa gcaaaataaa gcaggcgccc 120 tgggcaggtg gagcatggcc gatcatgggt cacctgcatc tactagcctg gggtgacctc 180 ccgcatataa cgttgggagc cttggccgat aagtacggcc cagtcttcat gatgcagctt 240 ggcatgcgcc aagcaatagt tgtaagcagt gcagagattg caagagagtg tttcaccact 300 aatgatagga tcttagcaac tcgcccaagc tctatagcag ttaagctcat ggcctacaac 360 tacgccatgt ttgcatttgc cccgtatggg cctaattggc gtgaaatacg caagacagtc 420 atgcttgagc tcctctccac ccctcgcctc gagtcactca agcatgtctg ggaggatgaa 480 atatccacga gtgtcaaaga gttgtatgaa ttatgtgcca gcaaaaccaa aacccatggc 540 catgtttcag tagacatgaa gcaacggttt ggtgatctga cactcaatgt gttagtgaag 600 ttggttaatg ggaagagata ctttggtgca aatactgatt atactgaaca tgaatcaagg 660 aggtgccaga aggcagtgag ggattttttc agtcttatgg ggttgtttgt ggtatcagat 720 tttcttccat ttcttgagcg gttggacttg cagggttatg agaaagagat gaaaagggta 780 gctagagaag ttgatggtat agttcaaggg tggttggacg agcataaaag gaagcgagag 840 tccagtgaaa ccacccacga tcaggacttc atggacgtga tgctttcgat acttaaagac 900 tcaaaattct ctaactctga ttatgatgcc gataccatca acaaggctac atgttttaac 960 atgatcttag gtggaagtga cacaacaatg agcacaatga cctgggcttt atcggcactg 1020 ctaaataatc cacatatgtt gaagaaggcc caagatgagt tggaattcca tgtcggaaag 1080 ggtagacagg tggaggaatc ggacatcaag aagctaacat acctacaagc tataatcaaa 1140 gaaactttgc gtttataccc gtcggctccc ctgttagctc cacgtgaagc cacagaagac 1200 tgcaaaatag cggggtatca tgttccagca ggcacacgcc taatcgtgaa tgtctggaag 1260 attcatagag acccatttgt ttggcccaac cctaacgagt tccaacctga aagattccta 1320 aataaagatg tggatgttaa gggtcagcat ttcgaattga tcccatttgg gtcagggaga 1380 cgtgcttgcc ctggggtatc attaggactt caagtggtgt cactgggctt ggcgcgcctc 1440 ctacacggat ttgagctttc gatcccatct ggaagcaagg tagacatgac tgaaactgca 1500 agtctagtta ctttcaaggc aacgcctctg gaagtattta tcactccacg cctatctcca 1560 catatgtatg tggagtag 1578 <210> 167 <211> 1511 <212> DNA <213> Mahonia aquifolium <400> 167 cttatgcatg gaagaggaaa aaccatacaa aggatagcaa aatcaaagag ccaccccaac 60 cagccggagc atggccgata atcggccacc ttcatctaat atcccgcggc ggcctccccc 120 atataaacat gggggccatg gcggacaagt acggtccagt tttcatgatc cggctaggag 180 taaatcgagc cgtgattgtg agtaactcgg agatcgtaaa agagtgcttc acaacaaacg 240 ataaattcct actaaaccgt ccagttgggt tggccttaaa cctcatgagt tacaacaacg 300 ccatgttcgg gttctctcgg tacggtccat attggcgtga gatacacaaa atagtcatgc 360 ttgagctcct ctcgagcagc cggctcgagt ccctgaagca cgtatgggac tcggaaatat 420 tgacatccat cgaagagttg taccaattat gtcaaacaca aaacaaggct catgaaggcc 480 cagttttggt tgaaatgaag gattggtttg cagatatggc actcaatgtg tcctttagaa 540 tggttgctgg gaaaagatac tttggtgcaa gtgctggtgg taataaagat gaggcaagaa 600 ggtgtcaaaa gaccatgaga gatttcttta gacttgttgg cttgtttata gtgtctgatg 660 cacttccatt tcttaggtgg ctagacttgg gagggcatca gaaggagatg aagagaacat 720 ttgaagatct tgactatgtg cttcaaggat ggttggatga gcataaattg aatagaaaat 780 ctggtgggaa tggtgatcaa gatttcatgg atgtgatgtt atctactctt tatgattcaa 840 aatttaccga ttacgacgtt gacaccatca acaaggctac gtgctttcaa ttgatcctag 900 gaggaactga tactacaacg gtcactctaa catgggccct ttccttgatg ttaaaccacc 960 cacatgtatt gaaaaaggcc caagatgagt tggacatcca agtaggcaaa cacagacaag 1020 tggaggaatc agacatcaag aacctaaaat accttgaagc tgtgatcaaa gaaaccttac 1080 ggatgcgccc agtaggccca cttttaggcc cacgagaggc gatcgaggac tgcaccattg 1140 gtggatatcg agttcgagca ggcacacgcg taatggtcaa tgcttggaag gtgcaacgtg 1200 acccatcggt ttggccaaac cctgatgact tcaaaccgga gaggtttctc aagaaggata 1260 ttgacttcag ggaccaaaac tttgaattca tccctttcgg gtccggtaga agggcctgcc 1320 ctggaatatc atttgccgtg gaactaytgc ccatggcttt ggcccgttta ctacatgggt 1380 ttgagcttaa gactcaattg ggttgcaagg tggatatgac agagcatgcc ggtttagttc 1440 atgctaaggc tacccctttg gaagttcttg tcagcccacg cctgtctcga gagttgtatg 1500 tatcgtctta g 1511 <210> 168 <211> 1590 <212> DNA <213> Menispermum canadense <400> 168 atggattcaa ctgatcatca cctggccctt gtaataccca ctcttctagc tacccctgtc 60 ttattgttct ccttctactg tctcctcttg agaccaagga gtctcaaaaa acctcgtact 120 aacaaaccac ctgaaccttc tggttcatgg cccatcatcg gccatctcca tctcttaagc 180 caaggcctac ctcacataac actgggagcc atggccgaca agctcggacc agccttctca 240 atccgcctcg gcacgcgccg agcactagtg gtcagcagct gggaggttgc taaggagtgt 300 tacaccacca acgaccgagc cttcgcgagc cgtcccagca gcatagcagt caagctcatg 360 agctacaaca actccatatt ggggttcgct ccgtacggac agtactggcg cgagctgcgg 420 aagatagtgg tgctccagct tctctcgaac cggaggctcg agtcgctgaa gaacgtttgg 480 gaatctgaga ttgatttgtc tataaaggag ttgtacgata catgggcaag taatagagga 540 ggagaagcgt taatggtgga catgaagcag tggtttggag accttacgat gaacatagtg 600 gtgaggttgg tggtggagaa gcgttgtttt gggagaagcg tgggtgacga tgagactgag 660 gcgaggaggg gccagagagc gctgaaagag tttttaaaac tggtgggttt gtttttggtg 720 gaagacgcgt ttccattttt gagttggttg gatgttcagg ggcatcagag agagatgaag 780 agaatagcga gagaactgga ctcgctgttt caggggtggt tggaggaaca taagaggaag 840 agaagcataa aaggtgaggc caaaggagat caggacttca tggacgtgat gttgtctgta 900 ctcgaggatt ctaccatcag cactgatata gaagatgaca cagttatcaa gtccacatgc 960 ttgactgttg tcttgggagg gagcgacagc acagtgggaa ctctcacttg ggccctctcc 1020 ctactcctaa acaacagaca cgaattgaaa aaggcccaag atgaattgga cgcctatgtg 1080 ggtaaggaaa gacaagtgga cgaatcagac atcaagaacc tcgtgtacct acaggccata 1140 gtcaaagagg ttctgcgact ctacccagcg ggcccactat caggcccacg agagtccgtt 1200 gaggacactg tggtagcagg ttaccatgtt cccaaaggca cacagctcat agtgaacctc 1260 tggaagattc aacgtgagcc gtccatctgg gccgaccctc tagagtttca accagagaga 1320 tttctcacga cccacaaagg catggacgtg tggggccagc acttcgaatt aatccctttt 1380 gggtccggga gacggtcgtg cccgggcaca gcgttcgccc ttcaagtgat tcacctcaca 1440 ttggctcgtt tgctacatgg gttcgagctc accacaccat gtggtgcacc tgtggatatg 1500 agtgagagcg ctggtctcat taatgtcaag accacgccag ttgaggttca cgtcgctcca 1560 cgcctccctc tcaagctata taaatcataa 1590 <210> 169 <211> 1590 <212> DNA <213> Menispermum canadense <400> 169 atgattttaa ttgcaatggg caaccacatt gaactgcaag atatcatact gtattccctt 60 ggattttttg ctgctactat tttatggaga atttttagta cctatgtcct tagaaggaac 120 agttgctcca gaccaccaga agcagccgga aaattgccgt taattggtca ccttcatctt 180 ctaggagcga acaagatatt acatcataca cttggagaca tggccgatcg acacgggcca 240 atcttttctt tgaatttggg aattaaaaga actcttgtga tcactagttg ggaagtagca 300 aaagaatgtt ttacaacaca ggacagggtt ttcgccactc gtccgaaatc tttagttgga 360 aaagtagtag gatataatag cacagttatg atatttcagc agtatggacc atactggcgc 420 gagatacgca agctagcaat gattgagctt ctgtcaaatc gtcgactgga aatgctcaaa 480 catgttcggg aatcagaggt taatcttttc atcaaggagc tatatgagca atggtcaagc 540 aatgagaatg gaagcaaggt tgtggtggaa atgaaggaaa ggtttggaga tctgacaaca 600 aatattgtgg tcaggacagt ggcaggtaaa aaatattctg gtactggtgt gcatggtaat 660 gaagagtcga ggcgatttca gaaggcaatg acagatttta tgcacttagc ggggctgttt 720 atggtttctg atgcacttcc cttgctcggt tggattgata cattcaaagg atacagggga 780 aaaatgaaca agacagcaga agagattgat catgtacttg gaagctggtt gaaggagcat 840 cagcagaaaa gaaaaaatat cagcattaat catttagacg aagactttat ccatgtcatg 900 ctctctgcca tggacgggaa ccagtttcct gacatcgaca ccgaaactgc catcaagggc 960 acttgtttaa gtcttatctt aggtggttat gatacaacat ccgccacact tacgtgggca 1020 ctctccctaa tcgtcaacaa ccatcatgtg ttgaagaaag cacaagatga aatggacaag 1080 tacgtaggaa gagatcgcca agtaaaggaa tcagatgtga agaatttaac ttatttgcaa 1140 gccattgtca aggaaacact gcgattatat ccagcagcac cactatcagt ccaacacgaa 1200 gcaatggaag attgcacggt ggcaggatgt aacattccag ctggcacgcg gttagttgtg 1260 aacctctgga agatgcatcg tgacccgaaa gtttggtctg accctctaga gtttcaacca 1320 gacaggttcc tgcaaaaaca tgttaatgtg gacatttggg gtcagaattt tgagctttta 1380 ccatttggat caggacggag gtcatgccct ggtatcacat ttgcgatcca ggtcctgcac 1440 ttgacacttg ctcaattgct tcatgcgttt caactgggaa ctgtctttgc ctcgcccatt 1500 gatatgactg aaagttcggg tgttactaac ccaaaagcaa ctccactgca agtaactctt 1560 accccacgac tgcctcctga agtctactaa 1590 <210> 170 <211> 1542 <212> DNA <213> Menispermum canadense <400> 170 atggattcaa cctaccaact tctctctgcc ctattgttca tcctctgcct ttactatctc 60 gtctgtaaac caatcaccaa aacaaaaaac gacgaagctc ctgtaccagc cggagcatgg 120 cccatcatgg gccacctcca catgctccgc ggcccaaacc tccctcacat aaccctttca 180 tccatggccg acaaacacgg cccggccttc acaatccgac tcggtacgcg ccgagccctc 240 gtagtcagca actctgatct cgcgaaggag tgcttcacag taaacgacaa ggccttctca 300 acccgcccga gcaccgtagc cacgaagctc atgggctaca acaacaccat gttcgggttc 360 gccccgtacg ggccgtactg gcgcgagttg cggaagatag tgatgaccga gcttctgtcg 420 agccgaagac tcgagtcgct cgcgagcgtt tgggcctctg agattgagtc ctcggttaaa 480 gagttgttcg cggaatgggc tgaaaacagg gcgaagggtc cagtggtggt ggagatggga 540 gagtggtttg gaaacttgac gctcaacata gcgcttcgaa tggtggtggg gaagaggtat 600 ttgagagaag agtcgcggaa atgcttgaaa gcgatgaggg attatccgga gctgtttggg 660 aggtttttgg tggaagacgc ggttccgttt cttgggtggt tggacttgat tcaggggtat 720 cagagagaaa tgaagaaaac agcgagagaa ctggactctg ttcttgaaaa atggctagtg 780 gaacataaag ataagagagg ttctggggag gggaaaggag agcatcagga cttcatggac 840 gtgatgatat cggtacttga agattcaaag ctcagttacg gagaggctga tacggtcaat 900 aagtcaactt gcctgaatct aatcttaggt gcaactgaca caacaatggt ggctctcaca 960 tgggccctct cacttctact caacaataaa caagtcctca aaagagccca acaagaacta 1020 aaatcccaaa tcggcaataa caaacaagtg tcccaatccg acatcaagaa cctactttat 1080 ctccaagcca cagtaaaaga agcactgcga ctttacccac cagagccgct atctggcccg 1140 cgagaggccc tcgaagactg taccgtcgcc ggctaccgtg tccgagccgg cacacggctg 1200 atagtgaacc tgtggaagat tcatcgagac ccgtcgatat ggcaggtccc tctcgagttc 1260 cggccggaga ggtttctcac ggcccacaaa gatgttgatg tgtggggcca gcacttcgag 1320 ctgatgccgt tcgggtcggg cagaaggtcg tgcccgggca tctcgtttgt tcttcaagtg 1380 gttccgttga tattggctcg tttgctacac gggttcgaac tcacaacgcc tggcgaggct 1440 ttagtggaca tgagcgagag cgcaggtctt gtgaacgcca aagtgacacc ccttgaagtt 1500 gttatttctc cacgcctccc gctcgagctc tttgcgggtt aa 1542 <210> 171 <211> 1605 <212> DNA <213> Menispermum canadense <400> 171 atgattatga tgttcatcga ctactactcc tcatggcttc cccaaactct actacttcaa 60 tcaattttac tagctgtttc cctagtcatc ttcatcaatc tctttctcac gaggaggagg 120 agctactcct ccaaatccca caccaatatc atacaccctc ccaaggccgc cggagccctg 180 ccggtaatag gccacctcta caccctcttt cgaggcctct ccgccggcgt tcctctctac 240 cggcaactgg atgccatggc cgaccgttac ggccccgcgt tcatcatcca ccttggcgtc 300 taccctaccc tcgtagtcac gtgccgagaa ctcgccaagg aatgtttcac tacaaacgac 360 caaacctttg ccacgcgccc cagcacgtgc gccggcaagt acatcggcta caactacgcg 420 ttcttcggct tcgcgcccta cggcccgtac tggcgcgaag ctcgcaagat cgccaccgtc 480 gagctgctct ccaactaccg cctcgactcg ctgcgccacg tgcgagaagc tgaggtgggg 540 cgcaacgtgg acgagctcta cgccctccac gcgtcgtcat caacgaataa gcaaaacatg 600 atgaagattg atatgaagca gtggttcgat caggtgacgc tgaacgtgat tttgatgatg 660 gtggtgggga agcggtgcgt gaccactggt gggaatgaag aggaggtgag agtggtgaag 720 gtgttgcacg agtttttcaa gcacctaggg actttgtcgg tatcggacgt ggtgccgtac 780 gtggagtgga tggatctgga cgggaatata gggaggatga agagcacagc caaggaatta 840 gattgtatat tagggagatg gttggaagag caccgccgtg agaggaggag tgatttcatg 900 gacgctatgt tggcgatggt ggaggggatt aagatacctt attatgacag cgacaccgtt 960 attaaagcca tttgcctgaa tctcctcaac gctggatccg atacccttgg catcacgatg 1020 acctgggcac tgtcattact ccttaacaac cgccacgtgt tgaagaaagt caaagatgag 1080 ctggacgtcc acgtgggcaa aaaccgacaa gtggaagagt tagacgtcaa gaacttggtg 1140 tacctccacg ctgtcgtcaa ggaaacgctg cgcctcttcc ctcccgcgcc acttggcgtg 1200 ccacacgagg ccatggaaga ctgtgtcgtg ggaggcttcc acgtggcaaa aggtacacgt 1260 ctcgtcgtga acgtgtggaa gctgcataga gatcctagcg tctggtccga ccctctggcg 1320 ttcaagcccg agaggttctt ggacaacaac acggttgacg ttagaggtca gcacttccag 1380 ttattgccat tcgggtcggg caggcggggc tgcccgggaa tcacgttcgc gcttcaggtg 1440 gcgcacctga cgctcgctcg attgctgcat ggattcgagt gggacacacc ggatggtgcg 1500 ccggtggaca tgagcgaggt ctcggtattg accacggcaa aaaagaaccc tgttgaggtt 1560 ttgttcactc ctcgtcttcc ggcagaggtc tacacgcaaa attag 1605 <210> 172 <211> 1572 <212> DNA <213> Menispermum canadense <400> 172 taccaattat tattccttca aaccgccatt gcaggcctac tgttcgcctt agcactacta 60 gtgcgccttc gcttctcgag gcgtaacaat ggcaagaaga agcaagcacc accagaacca 120 gccggagcac ggctcatcat gggccacatc cacatgctca acagtggcga gcagcctcac 180 aagaccctcg ctgcactggc cgaccaatac ggcccagtca tcaagctccg cctcggccta 240 cgcgacgcca tagtagtaag caattgggag atggccaagg agtgtttcac cactaatgac 300 aaaatcttcc ttagccgtcc ccagaccgtc gtgtcaaagt tcatgagcta caacctcaaa 360 atgctcggct tcgctcctta cggcccgtac tggcgtgaga ttcgcaagat agtggtgtct 420 gagctgctct cgaaccgccg gctcgagctg ctgaagaacg cgtggacctc tgagatcagc 480 tcgtgggtga aagagttgtt tgacgagtgg ggtgcgaagg aggcgagagg gcctgttgtg 540 gtggacatga ggcattggtt cgggaacttg gcgttcaata tagggatgaa tatggtggct 600 gggaagaggt tttttgggcc gagagtggtg tctgacgagg acgggttgcg gcgagtccag 660 caaggcctca cggatttctt taggctaata ggcgcgttct tgttggagga tgcggtgccg 720 tttctaagct ggtgggacag tcaggggtat cagaaggaga tgaaggacac tgcgagagag 780 atggatacac tgattcaagg attgttggac gagcataaac gtaggagatc atcaagctct 840 ggtgaggcca aaggggagaa ggacttcatg gacgttatgc ttaatattct tgaaggttca 900 aagttcccca gtggagacgc tgataatatc aacaagtcta cttgcttgtc tctaatgctc 960 ggcttgacgg acacaacaac ggcgaccctc acatgggctc tctccttgct acttaacgac 1020 ccgcgtgtct taaagaaggc tcgagaagag ctggatttcc acgtcggaaa gaacagactg 1080 gtggacacat cggacttaat gaacctccca tacatccacg ccatagtcaa agaagtgttg 1140 cgtctccacc caccggcgcc tctctccggc caccgtgaat ccttggagga cagtgtggtg 1200 ggcggctacc atgtccccaa gggcacccgc tttatggtga acgtctggaa gatccaccat 1260 gacccgaata agtggcccga ccctattgag ttccgacccg agaggtttct gaccacccac 1320 aaagatgtgg aagtgtgggg ccagaacttt gaactgattc cgttcagttc aggaagacga 1380 gtgtgtcccg gggcttcgtt gtctctacaa gtgcttccgt tcacactggc tcgagtgttg 1440 caagggttcg aagttgcaac accaggaggt gcgccggtgg atgtcacgga gagcaaaggc 1500 ctctcaactg ctaaagagac tccgcttgac gtcgtgatca ctccgcgtct ccctgaaaag 1560 ttctaccatt ga 1572 <210> 173 <211> 1587 <212> DNA <213> Nandina domestica <400> 173 atggattcac tcatttcatt tcaagcaata gttgggcttt tcatcttgat cctagtttcc 60 tatcaatggc ttggaaggtc tagaagtatt aagactaaca agcataacga agcaccggaa 120 ccagccggca gatggcccat catcggtcat ctacatttac tcggagggtc ggaccaatta 180 ctctatcgaa cgctagggtc catggccgat aaacttgggc cggcattcaa tatccggctt 240 ggtagccgtc gggcctttgt cgttaacagt tgggaggtgg ccaaggagtg tttcaccatc 300 aatgacaagg cccttgctag tagacccatc actgtggctg caaagctcat gggctataac 360 tatgcagtct ttgggtttgc accatacagc ccattttggc gagcaatgcg aaaaatagcc 420 acacttgagc ttctttctaa tcgtaggctc gagatgctca aacatgttcg gatctcggag 480 gtggacatgg gcttgaaaga aatttatggg ctttggtcca agaacaagga ttcaggccct 540 ttaatgattg aactaaacag gtggtttgag aacttgactc taaatatggt tgtgagaatg 600 gtggctggca agagatattt tggtgctgat gcatcatgtg atgagaacga agcgcagcgc 660 tgccagaagg caatcagtga attcttccgt ctcattggga tatttgtagt atccgacgcg 720 attccttctc tatggtggtt ggatttgcag gggcatgaga aggccatgaa aagaactgcc 780 aaggacctgg attctatact aggaggctgg ttggagcagc atcggtcgcg gagagtcaat 840 ggtaaggtcc caaccgaggg ggaacaagac ttcatcgatg tgatgttatc gctacaagag 900 ggcgatcatc tctctgattt tgagtatgat gctgatattg ccatcaaatc aacttgcctg 960 gcacttatcc taggtggcag tgacaccact gcaggtaccc taacttgggc tatctcattg 1020 ctactaaacc atccctatgc tttgaaaaaa gcccaagagg aattggacct ccatgttggc 1080 aaggacagac aagtctatga tcaagacatc aagaatctgg tatatcttca agccatcatc 1140 aaagaaacac tgcgcctata cccagctggt ccactattgg gcccaaggga ggccatggaa 1200 gactgcacca tatcagggtt cgatgttcga gctgggacgc gcttggtggt aaatgtttgg 1260 aagattcaac gagacccaaa cgtgtggtcg aacccatctg agttttcacc tgaacggttt 1320 ttgacaagcc atgtggatgt cgatgttagg ggacaaaatt tcgagctcat gccatttgga 1380 tcaggccgac ggtcttgtcc aggcgcttca tttgccctcc aggtcctgca tttgacatta 1440 gcccgttttc ttcatgggtt tgggttggca aacccattgg gaaaacctgt agacatgaat 1500 gagagcccag gactaactat cccaaaagca acacccctaa atgtgctgct cactccacgt 1560 cttgattcag agctatatgg ttgctag 1587 <210> 174 <211> 1563 <212> DNA <213> Nandina domestica <400> 174 atggaaacct ttcagttcct tggaattcca ttgtttggct tctttgcctt attatgcact 60 tactacctag tattgaggaa gccatcatcc actaagatca gggagccacc tcggccagcc 120 ggagcatggc caatcatcgg ccatctccat ttacttgccc ggggtgacct cccccacgta 180 actttgggga agatggctga taaatatggc ccagtcttca agttaaagct tggtgtccga 240 caagcaattg tggtaagtga ttgggaggtc gcaaaggagt gttacaccac caatgatcgt 300 gccttagcca atcgtccaag tggcctagga gcaaaaatca tgggctacaa ctatgctttg 360 attgggtctg ccccctatgg cccatattgg cgtgatttac gcaaaattat cacgcttgaa 420 gttctctcga gccgtcggct tgaatcactc aagcatgtat gggactctga aatatccaca 480 tctgtaaaag aattgtacca aatatgggca gatcaaaaca aagctcaagg ccaagtttct 540 gttgaaatga aacaatggtt tagtgatatg acactaaatg tggcagttag gttggctgtc 600 ggaaagaagt acttaggtgc aactgccgat tgtgaaaaga ataaggcagt gcaatgtcaa 660 aaggccatca ggaatttttt cagactcgct ggtttgtttg tggtggcaga ttaccttcca 720 tttctgggct ggttggactt gggagggcac gaaaaagaga tgaagcacac agcaaaagaa 780 ctcgattata tagctcaaga atggttggat gatcataaaa agaagagaac ctccaatcgg 840 acagtggacg agccacagga tttcatggat gtgatgctct cgatcttgga agaatcgact 900 ttcacgggtt atgatgttga tatcatcaac aagtccacat gttttgcatt gatcctaggt 960 ggaacagaca ctgtggcagt tactctgaca tgggctctct gtttattact gaacaatcca 1020 catgtactga aaaaggccca agatgagttg gacatccacg tcggcaagga tcgacacgtc 1080 aatgaatcag atatcaagaa cctcgtttac ctccaagcta tgatcaagga aacattgcgc 1140 ttatatccag ctggtccgct actaggtccc cgtcaggtca ttgaggactg caccatagct 1200 gggtaccacg tacgagcagg gacacgtgtg atagtcaatg cttggaagtt tcaacgagac 1260 ccgtcgatct ggtcaaaccc ttgtgagttc caaccggaga gatttctgga caaagacatc 1320 gatgtgaagg gtcaacactt cgaattgatc cccttcggag caggcagaag ggcatgcccc 1380 ggaatctcct ttgctcttca agtattacca ttggcattgg ctcgtttact acatggtttt 1440 gagcttaaaa acccatcgga aagccaagtg gatatgactg agacccccgg tatggttcat 1500 gccaagacaa cccctttgga agtacttatc acaccgcgct tgtctccaaa attttatgta 1560 taa 1563 <210> 175 <211> 1488 <212> DNA <213> Nandina domestica <400> 175 tggaagaggc caacctctgt taagtacagg gaggcacccc aaccagctgg cgcatggcct 60 atcatcggtc atctccattt actggcccgt ggagacctac accacataac tcttggagcc 120 atggccgaca agtacggccc agctttcatg atgcggcttg gtgtgcacca agcaatggtg 180 gtgagcgatt cggagtctgc gaaggagtgt tccaccacca acgatagggt cttggccact 240 cgaccaagta gcgtagctgt aaaacttatg ggctacaact atgccatgtt tgggttcggc 300 ccctatggat catattggcg tgaaatacgt aaaatagtta ttcttgaggt cctgtcaaac 360 catcggcttg agtcactaaa acatgtatgg aagtctgaaa tatccatgtc caccaaagag 420 ttgtaccaat tatggataaa ccaaaacaag gatgaaggcc cgactttggt tgaattgaag 480 caatggcttt gtaacatgac actaaatata ggagttagga ctgttgctgg gaaaagatac 540 tttggcgcaa gttcttgtga tgcaaatgaa tcaaacagga ttcaaagggc aatcagagat 600 ttctttagat ttgtaggttt gtttgttgtg tcagattttc ttccttttct agggtggttg 660 gacttggaag gttatcagaa ggagatgaag agtattgcta gggaacttga ttctatcctt 720 cagggatggt tggatgaaca cacaaggaaa agacaatctg gtgggaccaa cgaaaatcag 780 gattttatgg acataatgct ttcagttctt gaacattcga atgcattgac tcactatgat 840 gcagatacaa tcaacaaagc gacatgcttt acaatgatct taggtggaag tgatactaca 900 atggtcactc taacatgggc cctctccttg ttactgaaca atccacatgt cttgaaaaag 960 gcccaagatg aaatagatat ccaagtaggc aaggacagac ttgtggacga atcagatatc 1020 aagaacctag tatacctcca agctatagtc aaggaaactt tgcgtctgta cccagctgtt 1080 ccaataataa ctccacatga ggccatagaa gactgcacca tagctgggta tcatgttcca 1140 gcaggcacac gcctcattgt gaacgcttgg aagatacaaa gagacccatt gatttgggat 1200 aaccctggtg agttccaacc agagagattt ctctacaaag atgtggatgt gaaaggtaaa 1260 catttcgaat taatcccttt cgggtccggt agaagggtgt gccctgggat ctcgttggcc 1320 ctccaagtgg tgtcattggc catggcccat ctcctacatg agttcgacct tgcgaaacca 1380 tcagaaggca atgtggacat gactgagagt gttggtttgg ttaatgctaa ggcaacccct 1440 ttggaagtac ttatcactcc gcgcctatcc ccaaagtttt atgaatag 1488 <210> 176 <211> 1578 <212> DNA <213> Nigella sativa <400> 176 atgggttcct tcaaccaaat aaatccatct gttgtatact ttgctgcctt taccatcttc 60 ttcttcttct ttctctataa aatattatac aagagaagaa ccagtgcgaa gaccagagtt 120 gctcctgaac ctgctggtgc gtggcctttc ataggtcact tacccttgct atcccagcaa 180 aacttgcccc atgtaacatt gggggtgttg gctgacaaat acggaccagc tttcactgtc 240 caactcggct tacacaaagc tgttgtggtg agtagttggg aggttgcaaa ggagtgcttc 300 actgtcaatg acaaagtgct cgctactcgc cctagctcag ttgcagtgaa gattatgtgc 360 tataactatg ctgtgtttgg ctttggacca tatggttcct attggcgtga agctcgcaag 420 atagttgtac gggagcttct ctcaaaccat cggcttaatt tgctaaaaca tgttctggtc 480 actgaaatta gcatgtctat gaatgaattg tacacagttt ggcaaaagaa tgctaataat 540 gttagtggaa aagctttggt ggagatgaag caatggtttg gtgacttgtc attgaacatg 600 attgtcaaga tagttgctgg aaagcgatac ttccaagcta gtgcaaattc agatgaaagg 660 gatcagttga aaaggggagt gcaagatctc ttccatttgg ttgggctgtt tctggtgtca 720 gatgcacttc catttcttag ctggttagac attggtgggc atgagaaagc catgaggaga 780 actgccaagg agcttgacaa catactgggg agttggttgg atgaacataa acaggctaaa 840 ttggcagggg cgacaaaggg agagcaagac tttatggatg tgttgatgtc catactagag 900 gataacaata atctccctga gtatgaacca gatgttgtca gtaaagctat ttgcttgggt 960 atgattttgg gtggagctga taccacaaca attactctga cgtggattgt gtccttacta 1020 ctcaacaatc aacacatttt gaagaaagcc caagatgaaa ttgacagcaa agttggaaaa 1080 gatagacaag taaatgaatc agacatagaa aaactggtat acctccaagc aattgtgaag 1140 gagacattgc gtctgtaccc acctgctcct ctgtcaacac aacatgaagc catggaggac 1200 tgcaccatag ctgggtatca tgtccaagct ggtacaagat tgataacaaa tatatggaag 1260 attcaaagag atccaggggt atggccgaat ccttgcgagt tccagccaga gaggtttctc 1320 actacccatg ccaatgtgga gtcaaatgga aagcattttg agtttattcc atttggatct 1380 ggtcgacggg cgtgcccagg tatgtcactt gggcttcagg tggtgcacct gacattagca 1440 cgtctgctac aaggttttga attggaaact ccgttgagtg tggaagtaga catgactgaa 1500 agtgctggac ttacaaatct caaagtaacg ccccttgaag ttttaatcaa tccgcgtcta 1560 ccttcaaatc tatactag 1578 <210> 177 <211> 1554 <212> DNA <213> Nigella sativa <400> 177 atggatttga caagcatctt gatttgtatc tttcttttca tcatttctat ctgctttctg 60 tcatggaagc gatcaaggag tctttccgga accagagcag ctccggaacc agttggtgca 120 tggcctgtca taggccatct aaggctgcta atggggtcca aaacacctca tatgacctta 180 ggaaacttgg ctgacaacta tgggccggca ttcaccatcc ggcttggtac gaagaaaaca 240 ctggttgtaa gtagctggga ggtggccaag gagtgtctca ctaccaacga caaagccttc 300 gctggccgcc acagcactat ggcggtggag ataatggggt acaactatgc cttcttcagc 360 ttaggtccta gtggtcaata ttggcgtgaa attcgcaaaa taacagtttc tgaacttctt 420 tcaaatcgtc ggttggagtt acttaaacac atatggagct cagagattaa gacttctatc 480 aaagaattgt atgaaatttg tgcagcacaa agtcaatatg ggaaaaaaca gtctcctatg 540 gtagaaatgc agcaatggtt tagtcatttg aacttgaatt tatcagcaag aatggttgtt 600 ggaaaacgat attttgctga cggtgttgaa gaaaatgaag gcgatatcag acgatttcaa 660 aatgcaatca aggagttctt ttactgggct ggagcgttta taatagctga tacaattcca 720 tatctaaggt ggatggattt cttaaatgag cggtcaatga aaagaacagc aaaagaaaca 780 gaccatgtac ttgaaggatg gttgcaggaa cacaaacgaa acaggctgaa cggtgtaacg 840 aaggagcaag atttcatgga tgtgctgctg acagaacttg gtgacaaaga tctgtgtggt 900 tacacagctg atgttgtgaa caaggccact tgcctgaaca tgatttttgg aggaacagat 960 accacaaaaa cgggcatgat atggacttta tcattaattc tcaacaatcc acaagtacta 1020 aagaagttac aaaatgaaat tgacatccaa gttgggaaag acagacaggt tgatgattct 1080 gacataggga atctcgtgta cctacaagcc actgtcaagg aagcgatgcg cctgtatcca 1140 cctgctacca tatttggacg tgaatccatc gaggactgca ctgtggctgg gtaccacatc 1200 caagctggga cacgtttgat tgtgaatgca tggaaaattc aaagagatcc aaatgtatgg 1260 cccaatcctg acgaatttca gcctgagaga tttctgacaa accaagcaga tgtggacttc 1320 aggggtcaag attttgagct cattcctttt ggggctggga gaagggcgtg tcctggtatt 1380 tcattgacag ttcgggttac acatttgaca ctggcatgtt tgctacaagg cttcaacttt 1440 gaaaccccct tgaatgaacc tgtagatatg tccgagaaat atggtttaac caactcaaag 1500 gcaacccctc tgaatgttat actcacacca agactccctt ccaatttgta ctag 1554 <210> 178 <211> 1593 <212> DNA <213> Nigella sativa <400> 178 atggattcaa tatttcatat gtatgcacca attaatatcc ttgctgctac ctttgttttt 60 attgtcattt ctgtgtggtt tctgttatgg atgaagagat cgagaaagaa tagcaagagc 120 agaagagctc ctgaaccggt agggggatgg cccttgatag gtcatttgcg cttgttagtc 180 ggccctgagt tgccccatgt aatattggga agcttggctg acaagtatgg accagctctc 240 tccatccggc ttgggatgcg tcaagcattg gtggtcagta attgcgaggt agttaaagag 300 tgtctcacca ccaatgacag atgcttctcc actcgcccta gctctgtcgc agtagagtta 360 atgggatata actatgcgtc gtttggttta ggtccttacg ggggcttctg gcgtgaagtt 420 cgcaaaatcg caattcttga gctcctctca aatcatcgtt tgaagtctct taaacatgta 480 tggatatctg agattagtgg ttttgtcaaa gaactgtacc aattatgtgt agctaatggc 540 aatacaggaa ggcaatctgc tttggtggaa atgcgacagt ggttaaatga tttgacacta 600 aatgtgtctg tcagaatggt tgttggaaag cgatactttg gctctggtag tgaacatggt 660 ggtgaagatg tcaagcggtt tcaaaaggca attaaagatt tctttcgttt ggcaggaaaa 720 ttcacgctgg cagatgcaat accattccta aggtggttgg actttggtca tgaaagagcc 780 atgaagaaaa ctgccaaaga gttggactat gtacttgcaa gatggttgga tcaacatcaa 840 aagaacaaac tcaattgcga aacaaagcag gtggatcaag atttcatgga tgtcatgttg 900 actgtccttg gtgataaaga tctgtatggt ttcaaggctg atgttgtcaa taaggccact 960 tgccttaatt tgatcttagg tggggttgac acgacgtcag tgaccctaac atgggcattg 1020 tcattgctac taaacaatcc aaacattcta aacaaggcac aagaagaaat tgatgtccag 1080 attggaaaag atagacaagt tgatgacaat gacttgggaa aactagtgta cctagaggca 1140 attgtaaagg aaacaatgcg gttatatcca gcaggtccac tgtcaggtgc acgagctgcg 1200 atagaggact gcatggttgc gggatatcat gtaccaaaag gaacacgact gattgtgaat 1260 acatggaaga tacagagaga tccagagaaa tgggacaacc ctagtgagtt tcggccggag 1320 agatttctga tcaccaccac caatggccat gtggaagtgt ggggtcaaca ttttgagtac 1380 attccttttg gatcagggag aagaatttgt cctgccatct cattttcact tcagttggtg 1440 catttgacat tggcccgatt gctacaagcc ttcagctttc aaacaccatc aaatgcagca 1500 gtagacatga ctgagacccc tggcctggtc aatttcaaag ccaccccgct ccaaatttta 1560 ctcactcctc gacttccttc cagtttatac taa 1593 <210> 179 <211> 1584 <212> DNA <213> Nigella sativa <400> 179 atgctcagta ttcatgattc aaccatggtt tttctccaac tacaagctat ttgtggcatt 60 tttggcttca ttttcattat tacatggtgg acaagatgga aaagttccaa caaaatgaaa 120 gcaccagaag tagctggtgc atggcctgta attgggcacc tgcatctgct tgggggcggc 180 cgtcccttat accagctcct tggagacatg tctgataagt atggaccagc ttttaccctc 240 cgaatgggaa tccaaaaggc acttgtagtg agcagctggg aagttgcaaa agagtgtcta 300 accaccaatg acagagctct tgctacccgc ccatcttcgg ctggagggaa gtacatgggt 360 tacaacaacg cacttatccc cttcagccct tatgggccat actggcgaga catgcgcaag 420 attgctacac ttgagctttt atctaatcat agactagagg agttgaagca tgtccgagaa 480 atggaaatca acacttgtat cagtgacatg tacaaacttt gtcaggttga ggatggagtt 540 gaaattaaac caatttctgt agatctgagt caatggtttg cagatcttac cttcaatgta 600 gtggtgatga tgataacagg aaagagatat attgggtcaa ctgatgcagg tgacatgaat 660 gaaataagac attttcaggc agcactggtt aaatttatga ggctattgag aatctctctt 720 ctggtagatg tatttccagt tctacagtgg attaattatg ggggattcaa aggagtcatg 780 aaatcaactg ctagagatat tgattccgta cttgaaaatt ggctgcagga gcatcagagg 840 aaaagacttt cccctgattt caatggaaac catgatttta ttgacgtgat gatatctacc 900 ttagaaggca cagaattctc tgattatgac cacaatacta tcataaaggc aatatccatg 960 gctatggtcg tgggcggcac tgatactaca actacaacac ttatatgggc tatttccctt 1020 ctattgaata atcctaatgc gatgaaaaaa gttcaagaag agttggagat acatgtaggc 1080 aaggaaagaa acgtagacgg atccgacata caacatttgg tatacctcca agctgttgtg 1140 aaggaaactc tcaggctata tcctcccgtt ccactctcag taatgcacca ggccatggaa 1200 gactgtgtta tcggaagtta caacattcaa gcaggtacgc gtgtactttt caatctctgg 1260 aagctacacc gtgactcgtc agtgtggtct gatcctttag aatttcgacc agagagattt 1320 cttactagcc atgttgatgt agacgtcagg ggtcaacatt ttgaactgat accttttggg 1380 tctggaagaa ggtcatgccc tgggatttca tttgctcttc aggtcataca cttgacaata 1440 gctcgcttat ttcatggatt caatctgaca acacctggga attcatcagt tgatatgagt 1500 gagatttctg gagctacatt atcaaaagtt actccattag aagtactagt tactccacgg 1560 ctgtcttcca agctttacaa ttga 1584 <210> 180 <211> 1566 <212> DNA <213> Nigella sativa <400> 180 atggatttct tgatctacgt tgctgccttt gccgccgcct tcttcttctt attgttctac 60 aacatattat ccaagagaat aaccggtaca aagagaagaa ttgctcctga acctgctggt 120 gcatggcctt ttattggtca cttgcccatg ctcttcgggc ccaacttacc ccatgtaacg 180 ttgggtgcct tggctgacaa gtatggacca gctttcacta tccagctggg cttacacaaa 240 gctcttgtgg tgagtagttg ggaggttgcc aaggactgct tcactgtcaa tgacaaagcc 300 ctcgccactc gccctacctc tgctgcagtg aagattatgg cctacaactg tgccgtgttc 360 agtttctcac catatggttc atactggcgt gaagcacgca aaatagctat acttgagctt 420 ctttcacacc ataggcttga tttgttaaaa catgttcgga aatctgaagt aagcacatct 480 ataagagaac tgtaccaatt ttggcaaaaa aatgctacgg aagttactaa atttgctcag 540 ctggaaatga agcaatggtt tggtgatttg acaatgaacc tgattgtcag gatggttgct 600 ggaaaaagat atgctagtgt tcaatcagat gatgatggtg aggggaaaag gctacaaaag 660 gccgttaatg atttcttcca tctagtaggg ttgtttgtgg tatccgacgc acttcccttc 720 cttggttggt tagatattgg gggcaacttg aaaaccatga agaaaactgc cagggaactt 780 gacagcataa tggagtgttg gttgaaggam catcaagctc ggagatcaaa tggggagcaa 840 gatttcttgg atgtgatgtt gtccatactg cgtgaagata ataagctgca agagtatgat 900 ggtgatgttg ttgtcaaagc tatgtgcttg aatatgattt tgggtggagc tgatagcaca 960 acaatcactc taacatggac catctcctta ctacttaaca acagacacat tttgcagaaa 1020 gcccaagacg aaattgacag caaagtgggg aaagatagac aagtgaatga atctgacata 1080 gagaaactag tataccttca agcaattgtr aaggagactt tgcgtctgta cccacctgct 1140 cccctatctt cacaacatga agccatagag gactgcacaa tagctggcta ccacatccca 1200 gctggtacaa gattaataac aaatctatgg aaaatccaac atgatccaag tgtatggcac 1260 aatccttccg agtttcagcc agagaggttc ctcactaccc aggcgaatgt ggactttaga 1320 ggtcagaatt tcgagtttat tccatttgga tttgggcgac ggtcatgccc aggtacctca 1380 cttggtcttc aaatggtgca cctgccatta gcacgtttgc tacaaggctt tacatttgaa 1440 acaccatcaa atgcagctgt ggacatgact gaaagtgctg ggcttacaaa tcacaaagta 1500 acaccacttg aggttctaat atcaccacgt ctccgtttcg atctttatca agaatctccc 1560 ccttga 1566 <210> 181 <211> 1580 <212> DNA <213> Nigella sativa <400> 181 attccttcaa ccaaacatat actggtttta tctgcttatc tgtctttgcc tccttcttct 60 tcttcttcct ttacagcgtg cagcagaaga ggagaacagg tagaaaggcc agatacgctc 120 ctgaacctac tggtgcatgg cctttaatag gtcacttgcc aatcttgttc gaacctaact 180 taccccatat acagttggga gccttagctg acaagtatgg accagtgttc tctatcaaac 240 ttggcttacg gaaggctctt gtggtaagca gctgggaagt tgtcaaggag tgcttcactg 300 tcaaagacaa agtccttgcc tcccgcccta gctcagttgc tgtgaagata atgggctatg 360 attatgccgt atatgcattc ggaccatatg gatcatattg gcgtgaagct cgcaagttaa 420 ctatacttga acttctctca gaccatcggc ttgatttgct aaaacatgtt cgggtctctg 480 aagttagcat gtctatgaaa gaactactca aattctgcca aaagaatgcc aataatgtca 540 gtggaaaagc tttattagta gatatgaagc aatggttcgg tgacttgaca ttaaatgtta 600 ttgttaggat ggttgctgga aagagctact tgggtgctag tgcaaaattg gaagacggcg 660 aacagaaacg gttacaaaac gcaattcatg agtttttccg tttggcgggg ctatttgtgg 720 tgtcagatgc acttccaatt cttagctggt tagacattgg ggggcatgag aaagccatga 780 ggagaaatgc cagggagatt gacaagataa tgagcagttg gttggatgaa catcgacgca 840 ataaattggc cggggtaaca aatggagagc aagactttat ggatgtattg ttgtccaaat 900 ttgagaatag taatctccct ggatatgaac ctgatgttgc aatcaaggct atttgcttga 960 atatgatttt aggtgcagct gataccacaa tcgtcactct aacatgggtt gtgtctttaa 1020 tacttaacaa ccgccaaatt ttgaagaagg cacgagagga aattgagaat aaagttggaa 1080 aagataggca agtgaatgaa tctgatatag agaaactagt atacctccaa gcaattgtga 1140 aagagacgtt gcgtttatac ccagctgctc ctctatcagc acaccatgaa tccatggagg 1200 actgcaccat tgctggctac cacgtcccag ccggtacaca attaataaca aatttgtgga 1260 agatccaaag ggatccacga gtatggccta atccttgcga gttcctacca gagaggttcc 1320 tcaccacgca tgcaaacgta aactataagg gtcagaattt tgaattcatt ccatttggat 1380 atggtcgaag gtcgtgccca ggtatgtcat ttggacttca aatggtgcac ttagtattgg 1440 catctcttct acaaggcttt gaattggaaa ctccattgaa tgaggcggta gacatgactg 1500 agagcgctgg acttacaaat ctcaaagcaa cgcctcttga agttctaatt actccacgta 1560 tgcctttgaa tctatactga 1580 <210> 182 <211> 1530 <212> DNA <213> Nigella sativa <400> 182 gtaactgtta tctacttatt tacatttgcc atcttcttct acctctacag catattatgg 60 aagaacccaa gaaagagtgc aaagaccaga gttgctcctg aaccagctgg tgcatggcct 120 ttcatgggtc acttgcctat gctattggaa cccaacttgc cccatataaa gttgggagcc 180 ttagctgaca agtacggacc agccttcact gtccaactcg gcctccacaa ggctctagtg 240 gtgagcagtt gggaggttgc caaggagtgc ttcactgtca atgacaaagt cctcgcagat 300 cgccctagct cagttgcagt gaagataatg ggctatgata atgctgtttt cgcctttgga 360 ccatatggtt cttactggcg tgaagctcga aagatatcta cagttgagct tctctcgaac 420 catcgtctta gtttactgca acactttcga atttccgaag ctagcatgtc tatgaaggag 480 ctatatcaac tttgtgccaa taacgggaag gctttggtgg aaatgaagaa atggtttggt 540 gacttgtcat tgaatgtaat tgtcaggatg gttgccggaa agaggtatgc caaagctagt 600 gaagatgatg aacggagaca gttacagaag gggcttaaag atttctttta tttggtagga 660 ttgtttgtag tgtcggatgc acttccaatt ctaagttggt tagacattgg gggccatgag 720 aaagccatga ggagaaccgc caaggagctt gacaccataa tggagaggtg gttagatgaa 780 catagacggg ctaaattggc tggggtgaca aagggagaac aagacttcat ggatgtgttg 840 ttgtccatac ttgaagatgg caagcttcct cactatgaat ctaatacagt taacaaagct 900 atttgcttga ctatgatttt gggtgcagct cataccacaa cagtcactct aatttggaca 960 ttatcgttac tacttaacaa tcgacatatt ttaaacaagg ctcaagatga aattgattcc 1020 gaagttggga aggatagagc agtgactgaa tctgatataa agaatctagt atacctccaa 1080 gcaattgtga aggagacgtt gcgtttatat cctgctgcac ctctacctgc ccaccgtgaa 1140 gtcatggagg actgcatcat agctggttac cacgttccag ttggtacaag aataataaca 1200 aatttatgga agattcaacg agatcctcgt gtatggtcta atccttgtgc cttccaacca 1260 gagaggtttc ttactactca ttcaaatgtg gactttaggg gtcagaattt tgagttcatt 1320 ccatttggat ctggtcgaag atcatgccca ggtatctcac ttgggcttca aatggtgcac 1380 ttggcattag cgcgtctgct gcaaggattt gagttagaaa ctcctttgaa tctgggaata 1440 gacatgactg aaagttctgg acttacaaat cttaaagtaa caccgcttga agttgtgatc 1500 acaccacgac taccttcaaa tctatactga 1530 <210> 183 <211> 1689 <212> DNA <213> Papaver bracteatum <400> 183 atggcgtatt tgatgatcaa gaaatctttc cacttgtttt ccgatcaacc aacttcagtt 60 tccactctta tagtacttgc ttttctactg acactttcac ctgttattat ttactatgaa 120 cagaagaaga ggggtttgag gcgaaatcgg acctcatcgt catgtaccgc aattacaacg 180 actccgttac cagaggcatc aggtgcgtgg ccagtgatag gtcatcttct tcttttcatg 240 aacgaaaacg atttaaatca tgcaactctc ggtaacatgg ctgataaata tggacctatt 300 ttcagcttaa gattcggtag tcatagaact ctagttgtta gtagttggga gatggtaaag 360 gagtgtttta caggtgccaa tgacaagttt ttttcaaatc gtccttcctc cttggcggtt 420 aaacttatgt tttatgacac tgagtcttat ggctttgcac cgtatgggaa gtactggaga 480 gagttgcgga agatatctac acataaactc ctctctaatc agcaattaga caagttcaaa 540 cacttgcgga tttctgaagt ggacaactcc tttaaaaagc ttcacgactt atgcagcaac 600 aacaaactgg gaggagaaac tacatctgtg gctaatcttg tgagaatgga tgattggttc 660 gcttacttga cattcaacgt aataggacgg attgtcagcg gattccaatc aaatgcagtg 720 tcaggtgcca caagcagcca ggaaaaatac aagcttgcaa tcgatgaagt gtcaaatctt 780 atggcaacat ttgccgtttc agatgtggtt ccatgccttg ggtggattga ccgattgact 840 ggtcttacag gaaagatgaa gaaatgcgga aagaaattag atgcagtagt tggggatgca 900 gtggaggttc atcgccaaaa gaaactcaaa atttctagaa ataccgcagg agaacttacg 960 gagcatgaag aagaagactt catcgatgtt tgcttgtcga ttatggagca atcacagatt 1020 ccgggaaaca accccgaaat ctctgtcaaa tctattgcct tggacatgtt atcgggtggg 1080 agtgacacta caaagttgat aatgacatgg accctttctt tgctgttgaa ccatccagac 1140 atattggaca aggctaaaga agaagtagat acatacttca ggaagaaaaa gatatcggat 1200 aacacacctg tggttgatgc tgccgatgtt cctaacctcg tctacatcca agctatcatc 1260 aaagaatcaa tgcggttata ccccgccagc acattgatgg agcggatgac cagtgatgat 1320 tgtgaggttg gtggcttcca cgttccagct ggaacccgac tatgggttaa cgtatggaag 1380 atgcaacgag acccaagggt ttggaatgat ccattggtat ttcgacctga gagattcttg 1440 agcaatgaca aagggatggt agatgtgaag ggtcagaatt atgaactgat accattcgga 1500 acaggcaggc ggatatgtcc cggtgcatct tttgccttag aagtcttgca tttggtcctc 1560 actcgtctta ttcttgagtt cgagatgaag gcaccagagg gggaaattga catgagggca 1620 agaccaggtt ttttcaacaa caaggtagtg ccactagatg ttctactcac cccacgcaca 1680 ctagattaa 1689 <210> 184 <211> 2561 <212> DNA <213> Papaver bracteatum <400> 184 atgaataact actcatcatc acctgcatca tcaacagaaa cagcggtact ttgtcatcag 60 cggcagcagt cgtgtgcatt gccaatttcc ggtcttcttc acattttcat gaataaaaac 120 ggcttaattc atgtaactct tggaaatatg gcggataaat acggaccgat tttcagtttc 180 ccaacaggta gccatagaat tctcgttgtg agcagttggg agatggtaaa agagtgtttt 240 acaggcaaca atgacactgc tttctcaaac cgtcctatcc cgttagcttt taagactata 300 ttctatgcat gccgtggcat agactcatac ggtctttcga gtgtacctta tggaaaatat 360 tggagggagc tacgaaaggt ctgtgtgcat aacctcctct ctaatcaaca actactcaag 420 ttcagacact tgataatttc tcaagtcgat acgtctttca ataagctgta tgagttatgc 480 aaaaactctg aagacaacca gggaatggtg agaatggatg attggctcgc ccaattatcg 540 ttcagtgtga taggaagaat agtctgcgga ttccaatcag accctaagac aggtgctcca 600 agcagggtgg aacaatttaa agaagcaatt aatgaagcat cttattttat gtcgacatct 660 ccagtgtcag ataacgttcc gatgctaggg tggattgacc aattgactgg tcttacgaga 720 aatatgacgc actgcggaaa gaaattagac ttggtggtag agagcataat taatgatcat 780 cgtcaaaaga gacgattctc tagaactaaa ggaggagatg agaaggacga tgaacaagat 840 gacttcatcg acatttgctt gtcaataatg gagcaaccac agcttcctgg caacaataac 900 cctcctaaga tacctatcaa atctattgtc ctggacatga taggtgcggg gactgacacc 960 acaaaactga ccatcatttg gaccctttcc ttgctgctga acaaccccaa tgtgttggcc 1020 aaggcaaaac aagaagtgga tgcacacttt gaaacgaaaa agagatcaac aaacgaagca 1080 tcagtcgtgg tggatttcga tgatattggg aaccttgtct acatccaggc aatcatcaaa 1140 gaatcaatga ggttgtatcc ggtcagccca gttgtggagc gactgagcag cgaagattgt 1200 gtggtcggtg ggtttcatgt accagcaggg acgagattat gggctaacgt atggaagatg 1260 caacgagacc ctaaagtatg ggatgatcca ttggtgtttc gaccagagag atttttgagc 1320 gatgaacaga agatggttga tgtaaggggt caaaattatg agctgttacc atttggagcc 1380 ggtcgacgta tatgtccagg tgtatccttc tctctggatc taatgcaact ggttctgact 1440 cgtcttattc ttgagtttga aatgaagtct cctagcggga aagtggacat gacagcaaca 1500 ccaggattaa tgagttacaa ggtggtccct cttgacattc tgctcaccca tcgtcgcata 1560 aagtcgtgtg tgcagttagc atcctctgag agagacatgg agagtagtgg tgtaccagta 1620 atcactctga gatcgggcaa ggtgatgcct gttttaggca tgggaacatt tgagaaagct 1680 ggtaaagggt ctgaaagaga gaggttggcg atattaaaag cgatagaggt gggttacaga 1740 tacttcgaca cagctgctgc atacgaaact gaagaggttc ttggagaagc tattgctgaa 1800 gcacttcaac ttggcctaat caaatctcga gatgaacttt tcatcagttc catgctctgg 1860 tgcactgatg ctcaccctga tcgtgtcctc ctcgctcttc agaattctct gaggaatctt 1920 aaattggagt atgtggatct atatatgtta cccttcccgg caagcttgaa gccagggaag 1980 ataacgatgg acataccaga ggaagatatt tgtccaatgg actacaggtc tgtatggtca 2040 gccatggaag agtgtcaaaa ccttggcctc actaaatcaa tcggtgttag caatttctcc 2100 tgcaaaaaac ttgaggaatt gatggcgacc gccaacatcc ctccagccgt gaatcaagtg 2160 gagatgagcc cggctttcca acaaaagaag ctgagagagt attgcaacgc aaataatata 2220 ttagtcagcg cagtctctat actgggatca aacggaaccc catggggctc caatgcagtt 2280 ttgggttctg aggtgcttaa gaaaattgct atggccaaag gaaaatctgt tgctcaggtt 2340 agtatgagat gggtttacga gcaaggcgcg agtcttgtgg taaaaagttt cagtgaagag 2400 agattgaggg aaaacttgaa catattcgac tggcaactca ccaaggaaga caatgaaaag 2460 atcggtgaga ttccacagtg cagaatcttg agtgcttatt ttttggtctc acctaaaggg 2520 cctttcaaat ctcaagaaga gttgtgggat gacaaagctt g 2561 <210> 185 <211> 1623 <212> DNA <213> Papaver bracteatum <400> 185 atggagttct tgatgaagtt attattgtta cttgaaccaa tcacttttag tatttttctt 60 ggtataggtt ctattgttct tctatacaat gtcttcttct tggttattaa caagaaaaag 120 aagaagaaag caccaaatgc atcaggggca tggccgttga taggccatct caatcttttc 180 atgaatgata aggaagcgtt gtataaaaca ctaggaacca tggccgacaa gtacggacct 240 gcattcaacg ttcgattagg caaccaagaa atccttgtgg tgagtaattg gaagatggta 300 aaagaatgtt ttaacactca aaatgataag ctattttcga atcgtcgaac tacattgggt 360 gtgaaataca tgcttaacaa gaagacctct gttgcctttt caccatatgg aacatattgg 420 agggagctac gaaagctaac ggtgcaacaa ttgctctcta agcaacgttt agattcgtgg 480 aaacatttga aaatcaaaga aatagatgct tcatttggta gacttaacga tttatgcagc 540 aacaacaagg gtactggagc agctacccca attaggatgg acagttggtt tgccgagttg 600 acgttcaacg tgttcgcaag aattgtcttt ggctaccaaa gtggagaaag gttgatgcta 660 tcaggtgata cggcatccaa cggggagagg tacaagaaaa cattagaaga agcatttctc 720 cttatgtcaa gctttgcggt ttctgacgta ttcccatgct tagagtgggt agacagatca 780 agaggccttg taaggagcat gaaacgcttt ggagatcagc taaattcaat tgcagggtgt 840 cttattgagg agcatcgcca aaagagatca caatccgtat cagcatcaaa ttctacaaat 900 gataaaggag ttggtgatga acaagacttc attgatgttc tcttatcggt tgctgaacta 960 tcacaaattc ctggagatga ccctgatttg gtcatcaagt ctatgatttt ggaagcctta 1020 gcaggtggga gtgacacaac aacatcaacc ctaacttggg ttctttcact gctattgaac 1080 caccccaaag tgttaaagac ggcaaaagag gaaatagata tgcacgtcgg acgtaatcga 1140 tgcgtagaag agtcagatat tcccaagctc gtttatgtca atgcaattat caaagaatca 1200 atgagattgt atccaaatgg gtcattggtt gatcgattga cgttggaaga gtgtgaagtt 1260 ggtggattcc atgtcccagc tgagggacac ttattcgtaa acgtttggaa gatccataga 1320 gatccgagtg tgtgggagaa tcctctggag ttcaagccag agagattttt gagtaatgat 1380 tgcaaggtgg atatggattt cataagtcaa aaatatgaat tcataccatt cgggataggt 1440 cggaggatat gtcctggtat gctttcagca ttacaggtga tgcattttgt ggtagcccgt 1500 cttattcatg ggtttgatat ggaagcagca agtgccgatg ggaaagtgga tatggcagaa 1560 aagccaggca tgacttgcta taagatgaca cctcttgaag ttatgctcac ggctcgacag 1620 tag 1623 <210> 186 <211> 1647 <212> DNA <213> Papaver bracteatum <400> 186 atgtatccgg taaaccagtt gcagagtcaa gcaattgctg tgctatgtgc cgtaattgtc 60 ttcatcttct atttggggag aaagttgttt aacagtaccc atattcataa gcctggaaag 120 acagcaccag aaccagcagg cgcatggccg attataggtc atcttcacct ccttggggga 180 gcaaaactca tgtaccgaac cctaggttct ttggcggacg aatacggacc agttttcatg 240 gttcgacttg gtatgcgacg ggttcttgta attagtaaca cggcgtcagc tagagagtgt 300 ttcactacaa atgataaggt ttttgctacc cgcccaacca cagttgctat aaagttgctg 360 acttacaatc acaccatgtt tggttttgca ccttatggac cttactggcg cgagatacgc 420 aagatagcaa caacggagct cctatcggat agacgtctag ccatgctcaa aaacgtgtgg 480 atctctgaga ttaatttgtg cataaatgaa ttacatcaac tcttgatgga caagaatagt 540 agaaaagtag atcatcatca acatgtttac aaccaaaata tggacccaat ttcggtggag 600 atgaacggat ggtttgcaga tttgtctttc aatgttgtgg ccagaatgat agctgggaaa 660 agatatttcg gaaagaacac cgatggtggc gaagaagaaa ccaggaggta tcgggaagca 720 atgaatgatt ttatgcactt ggtagtgata ttattggtgt ctgatgctgt tccattttta 780 gggggtttag acttccaggg atataagaaa aggatgaaga aaactgccaa ggaaattgat 840 tattttctgg gtaaatgggt tgatgaacat cgacagcgtt tgaaatataa aaacatatcc 900 aaggttgatc aacaagatga tttcattgat gtgttactgc tgaatttaaa tgatcaaccg 960 atctatggtc gcgatactga tacaatcatc aagtctactt gcctgtctct tatcgcaggt 1020 ggtagtgaca ccacagcagt taccctaact tgggcactat cgttactgct gaacaaccaa 1080 cacgtgttaa gaaaggctca ggacgagttg gacatacacg taggcaggga aagacaactg 1140 gaagaatcag atatcaagaa cctcgtttat ctccaggcca tagtgaagga aacattgcgt 1200 ttatatccag ctgcaccgtt atcagcacca aggatggcaa tggaagattg tacggttgcg 1260 ggattccaag ttagtaaagg cacacagctg atgcttaatg tatggaaact acatcgagac 1320 ccgcattttt ggggacctga tcctctggag tttaggccag agagatttct tactgctgat 1380 ggtagtagca cgggaagtgg tcattgcatt gatattgatg ttaagggtcg gcattatgag 1440 ttactaccat ttggatctgg tagacggatg tgcccaggag tttcatttgc catgcaagtc 1500 gttcatctga cgctcgctac tttacttcac ggatttcatt tatcgacgcc aacagatggt 1560 ccagttgata tgactgaaac ttcaggactt agttgcccca aagcaacccc attagaagtt 1620 ttgctcactc cacgtctgcc ctgttag 1647 <210> 187 <211> 1704 <212> DNA <213> Papaver bracteatum <400> 187 atggatgtgg caatcatcgt cgatcaccac tacttgcagc cgttcgtgag tattgcaggc 60 ttattagctt tgctatcctt cttctattgt atttgggtct ttattataag gccaaggatt 120 atcaaaagca acttagacga gcgcaaatta tcaccatcat ctccacctga agttgcaggt 180 gcatggccga tagtagggca tcttcctcag ctaataggat ctacacctct attcaagatc 240 cttgcggata tgtctaacaa gtacggacct attttcatgg tcagatttgg tatgtatccg 300 accctagtag tgagcagttg ggagatgtca aaagaatgct tcactaccaa cgataggtta 360 ttcgctactc gtccacctag tgcggccgga aagtacctca ctaaagcctt gtttgccttt 420 tctgtgtatg gtccttactg gcgggagatc cgtaagatct ctacaatcca tttactctca 480 cttaggcgcc tcgagttact taagcatgga cggtacttag agatcgacaa atgcatgaaa 540 agattatttg aatattggat ggaacatcat aagaacatca taagcacaac tagttcagtt 600 aaggtcaaca tgagccaagt gtttgcagaa ctatccttaa atgtggtttt gaagataata 660 gttggaaaga ccctttttat taaaaatggt aatgaagatt acaccaaaga ggaagaagaa 720 ggccaaaagc tccacaagac tatcctaaaa ttcatggaac tagcgggggt atcagttgca 780 tctgatgttc ttccattcct tgggtggttg gatgtggatg ggcaaaagaa acaaatgaag 840 agggtctaca aggagatgaa cttgattgct tcaaagtggc ttggagaaca tcgagagaga 900 aaaagactgc aaataataca aaaacgcgga gcagcaagag gcagtaatta tgatgatggg 960 aatgacttca tggatgtgtt gatgtcaatt cttgatgaag aaaacgatga tctcttcttt 1020 ggttacagtc gagatactgt catcaaatct acatgtctgc aacttatagt ggccgcttcg 1080 gatacaacgt cacttgcaat gacatgggct ctctcactgc tgctcaccaa tcctaatgtc 1140 ttacagaagg ctcaggatga gcttgatacc aaagttggca gggacagaat aatagaggaa 1200 cacgatatcg agtgtctcgt ctacctccaa gcaattgtca aggaaacact ccgattgtac 1260 cctcccgctc cactctccct accccatgag gctatggaag actgcactgt tggtgggtac 1320 caagtgaaag caggcacacg cttagtagtt aacctgtgga aactgcagcg tgatcctcga 1380 gtgtggtcaa acccgttgga gtttaagcca gagaggtttc ttccacagtc agatggtggt 1440 tttggtggtg aagaagcaag aatggacttc aggggacaac attttgagta cacgccattt 1500 ggatcaggga gaaggatatg cccaggtatc gactttttcc tccagacagt tcacatggca 1560 ctagctcgtc ttcttcaggc atttgatttc aacacagctg gaggactagt tatagacatg 1620 gtggaaggtc caggtctaac catgcccaaa gtaaccccac ttgaagttca cctaaaccca 1680 cgtctgccgg tcacacttta ctag 1704 <210> 188 <211> 1677 <212> DNA <213> Papaver bracteatum <400> 188 atgcaggtgg attggccaaa catcttacag aagtactacc caataataac atgttcatta 60 ttaactttgc tatccttcta ttatatatgg gtctccatca caaaaccaag caggaattca 120 aaaaccaaat taccgccacc tgaagttgca ggttcatggc ctatagtagg tcatcttcca 180 cagctagtag gatctacacc tttgttcaaa atccttgcta acatgtctga caaatatgga 240 cctatcttca tggtccggtt tggtatgcac ccaaccttag ttgtgagtag ttgggagatg 300 tctaaggaat gcttcaccac caatgacaag ttcctcgcca gtcgtccacc tagtgcttca 360 gccaagtacc tcggttatga taacgccatg tttgttttct cagattatgg tccttattgg 420 cgtgagatac ggaagatctc tactctccaa ttactcacac acaagagact cgattcgctg 480 aagaatatcc cttacttaga gatcaacagc tgcgtgaaaa cgttatatac ccgttgggcg 540 aaaacccaaa gccagataaa gcaaaatgtt ggaggtgcag ctgacgattt tgttaaagtc 600 gacatgacag aaatgtttgg acatctaaac ttaaatgtgg tgttgaggct tgttgtagga 660 aagcctattt ttatccagaa agacaatgct gatgaagact acacaaagga tggccataac 720 aaagaggaat taggtcaaaa gcttcacaag actatcatag agttctttga attagcaggt 780 gcttcagtcg catctgatgt tcttccatat cttgggtggc ttgatgtaga tggacaaaag 840 aaacgcatga agaagatagc catggagatg gacttatttg ctcaaaaatg gcttgaagag 900 caccgacaaa aaggaatcaa tcatgataat gagaatgact tcatggccgt gttgatatca 960 gttcttggtg aaggaaagga tgaccacatc ttcggttaca gtcgggacac tgtcataaaa 1020 gccacatgtt tgacacttat cgtagcggct acagacacca ctttagtgtc attgacatgg 1080 gctctctcac tactactcac caatcctaga gtcttaagca aggcgcaaga tgagcttgac 1140 acagtagtcg gcaaggaacg aaacgtagaa gatcgagacg tcaatcacct ggtctacctc 1200 caagcagtca tcaaggaaac tctccggttg taccctccta gcccactcgc cgtaccccat 1260 gaggctattg aaaactgtaa tgtgggcggg tatgaagtga aagcaaggac acgtttattg 1320 gttaacttgt ggaaaattca tcgtgatcct agagtatggt caaacccgtt ggagtttaag 1380 ccagaaaggt ttctcccaaa actggatgga ggtactggtg aagcttctaa gttggacttc 1440 aaaggtcaag attttgtgta tacaccattc ggttcgggac gaaggatgtg cccagggatc 1500 aactttgcca gtcagacgct tcacatgacg ctagctcgcc tactccatgc attcgatttt 1560 gacattgagt caaatggact agtaatagac atgacggaag gttcaggttt aaccatgccc 1620 aaagtaaccc cactccaagt tcaccttcgc ccacgtctcc ctgccacact ttactag 1677 <210> 189 <211> 1716 <212> DNA <213> Papaver bracteatum <400> 189 atgatggatc tagcaatgtt catcgatcaa tacttctcat tagctaaaat tgcaggctta 60 ttagctttgt tatctttctt ctattatcta tggatctcaa ctttgtggtc gccaaggaat 120 cccaaactat catcagtatc accacctgaa gttgctggtg catggcctat attaggccat 180 cttcctcagc tactaggatc aagacctcta ttcaaaatcc tagcagacat gtctgacaat 240 tatggtccga tcttcatggt ccggtttggt atgcatccga ccttggtggt aagcagttgg 300 gagatggcta aagagtgctt caccaccaac gacaggttcc tcgctggccg cccatcgggt 360 gctgccaaca agtacctcac gttcgccctg tttgggtttt ccacatacgg cccttactgg 420 cgagagatcc gtaagatcgc taccctccat ttactctcac ataggcgtct cgagcttctc 480 aagcatgttc ctgacttgga ggtcaccaac tgcatgaaac acttgcatcg acgctggata 540 gacagtcaaa accaaataaa acaaaatgac gctgcagctg gttcagtaaa agttgacatg 600 ggcagagtat ttggcgagct aaccttgaat gtggtgttga agttagttgc tggaaaatca 660 atttttttca agaacgacaa cactcgtcaa tatgactcca aggatggtca taacaaagag 720 gaggaagaag gtaaaaagct ccataagacc atcatagatt tctactcatt agcaggagct 780 tcggttgcat ctgatgttct tccatttctt gggtggttgg atgtggatgg acaaaagaaa 840 cgcatgaaaa gggtagccaa ggatatggat ttcatcgctg caaagtggct tgaagagcac 900 cggcatcaaa aaagacaaac agtactatca agctcagcaa cactaggcag cagtaatcat 960 gacgatgcga aagatttcat ggacgtgttg atgtcaattc ttgatgggga aaatgatgat 1020 ctcttctttg gttacagccg tgacactgta atcaaaacca catgtctgca acttatagca 1080 gctgccgcag ataccacatc agttacgatg acatgggctc tcgcactact aattactaat 1140 ccgaccatat taagaaaggc tcaggatgag cttgatacca aagtaggcaa ggacagaaac 1200 atagaggaac gcgacatcaa tgatcttgtc tacctccaag caattgttaa ggagacactc 1260 cggatgtacc ctgctggccc actcaacgta ccccacgagg ctatcgcaga ctgcaatatt 1320 ggtgggtatg aagtgagagc aggcacccgt ttgttggtaa acctatggaa aatgcaccgc 1380 gatcctcgag tatggtcaaa tccgtcagag ttcaagccag agaggttcct tccacaactg 1440 gatggtggat ctggtggtga ggcggcaaac ttggacttca ggggtcaaga ctttgagtat 1500 ctacctttta gtgcaggaag gaggatgtgt ccagggatcg acttttccct ccaaacgctt 1560 catatgactc ttgcgcgcct gcttcatggg ttcgatttca acaatgactc ggcaggaata 1620 ataatagaca tggaggaagg ttcaggttta actatgccca agttaacacc actagagatt 1680 tacctctgcc cacgccttcc agccaaactc tactag 1716 <210> 190 <211> 1632 <212> DNA <213> Sanguinaria canadensis <400> 190 atggagtttc tttctctaca attccaaccg attaccattt ttgcccttct tcttgccact 60 cccatttttc tgtacaatct ctggaattat ggaaggaaac tcaacaacaa gaacaagaag 120 agcagaaaac caccagcacc tgaagcatca ggtggatggc cgatcatggg tcattttcat 180 cttttcaatg gaaccgaggt gactcaccga gctctcggtt ccatggcgga caaatacggt 240 ccagctttca atatccgaat cggttctcat ccaacacttg ttgtgagtag ttgggagatc 300 gctaaggaat gtttcaccac aaacgaccga ctcttctcaa ataggccagg ttctttagcg 360 attaaactca tgttctacga tgccgattcg gttggttatg caccgtacgg aacttactgg 420 agagagttga gaaagatttc aactctaaaa cttctttcaa atcacagatt ggaaacgctt 480 aaacatttga gaacatctga agttgaatcc tgttttaagc agcttcatga tttgtggcgg 540 atgaacaata aaaagggggg gagcgatgat ggatcagatt ttgctctggt gaggatagat 600 aattggtttg gtgatttgac ttttaatgtt gtggcgagaa ttgttgccgg gaaaaagaat 660 tttgcgggag gtgcggcgag tggtgatgcc ggagctcaaa gatataagga agccatggat 720 gaagcgtttc gtttgatgac gaggtttgcg ttctcagatg ttgttccatc gcttggatgg 780 ttagataaat tgacaggact tgttggagga atgaaacgtt gtggatctga aattgattcg 840 atcgttgcaa gttgggtgga tgaacatcgg ttgaaaagaa tttctggaaa agaaggggat 900 gatcttgaac aggatttcat cgatgtttgc ttagagattt tggagcattc tactttgcct 960 ggcgatgatc ctgaagttgt catcaaatct acttgcctgg acatgatatt gggtgggagt 1020 gacaccacaa cagtgaccct aacatgggcc ctctccttac tattggacca tccccacgtg 1080 tggaaaaggg cgaaggagga agtggatgca cacgtgggaa aggagagaca ggtggaggac 1140 tcagatatcc ctaatcttgt gttcatccaa gcaatcgtca aagagacgat gagattgtac 1200 ccagctggac cactgatcga gcggaggaca atggatgatt gtgaggtagg tgggttccac 1260 ataccaggtg gcacacgttt aattgtgaat ttatggaaga ttcaaagaga cgggagcgtg 1320 tacaaggagg atccattgga gtttagacca gagaggttcc tgacgagcaa cgcggaggtg 1380 gatctaaagg gacagaatta cgaactgata ccatttggtg ctggaaggag gatatgtcct 1440 ggagtgtcgt tcgcagtgca attgatgcat ttggtactgg ctcgtctcat tcacgacttc 1500 gaaataacga tgcccatggg tgcgaaagta gacatgactg agagtggagg actaatcagc 1560 cacaaggtga cgccgctgga agtgctgctc aaaccacgcc tcccctcgct ccaacaagca 1620 gctttatatt aa 1632 <210> 191 <211> 1578 <212> DNA <213> Sanguinaria canadensis <400> 191 atggaatatt cctcagtact tctccattgg ttccctgctt ccatggctgc ccttcttgcc 60 ttggtttttc tgtacaatct catctttaat tccccaaaaa ccggtaataa gaagattata 120 aggagggcac ccaaagcagc cggtgcatgg ccagtccttg gtcacctcca tttgtttgga 180 tcaggtgagc tgcctcacaa aatgctcgca gccatggcag aaaaatacgg ccccgccttc 240 acaatgaaat tcggtaagca cacgacacta gttgtgagtg acacccgcgt cgtaaaagaa 300 tgtttcacca ccaatgacac cctctttgcc aaccgtccat ccaccaaagc ctttgatctc 360 atgacttacg ccaacgactc ggtagccttc acaccttaca gtccttattg gcgcgagctt 420 agaaagatat ccactctcaa acttctctct aaccaccgcc tccaatcgat caaggaagtc 480 cgagtctccg aggtgagcgt atgttttagg gagctatacg atatatgcaa aaatgatgga 540 ggaggagctc ctccagtttt ggtggatatg aagaaatggt tcgaagaggt ctcgaacaac 600 atagtgatga gggtaatcgt agggaaacaa aattttgggt cgaagattgt ttgtggcgaa 660 gaggaagccg tcaattacaa gaacgtcatg gatgagctcc tacgtcttgc tagtgtgtct 720 atgttatcgg atgtggcacc tttacttggt tggttggata tgttccaagg acacatgagc 780 gccatgagac gaaatgggaa aaaactagac accatacttg agaggtggtt ggaggagcat 840 cggaagaaga agagtgagga tgagcaagat ttcatggatg ttatgttgtc gatcgttgag 900 gagactaagt tgtctggccg cgacgctgat accgtcatta aagctacttg tctggccatg 960 atcatgggtg ggacagacac cacggctgtg agtctaacat ggatcgtttc cttattgatg 1020 aacaatcgct atgcattgaa aaaggctcga gaagaattgg acgcgctcgt ggggaaagat 1080 agacaagttg aagattcaga tttgaagaat ttggtgtact tgaatgccgt cgtcaaggaa 1140 acgatgcgat tatacccgtt gggtactctt cttgaacgtg acaccaaaga agattgtgag 1200 gttggtgggt tccatgtgga aggcggtacg cgtttactag tgaatgtatg gaagttacaa 1260 cgagacccaa acgtgtggaa tgatccatta gaatttagac cagagagatt tctaacagag 1320 aatgcggata tagatgtcgg aggccaacat ttcgaattac taccatttgg ggcagggaga 1380 agggtgtgcc caggagtgtc gttcgcactc caattcatgc atttggtatt ggctcgtctc 1440 atccatggct atgaattggg aacccaaaat gatgaggatg tggatctaac tgagagcaca 1500 gaaggacatg ttaaccacaa agcctccccg ctcgatctcc tcatcacccc acgcctccac 1560 cctaaacttt atgaataa 1578 <210> 192 <211> 1584 <212> DNA <213> Sanguinaria canadensis <400> 192 atggattatt catcccattt gaaccagttc tacggggtag ctccatacat ggctgcccta 60 ctaattgcct tagtgtttct ctacaatatt ctttgggctt ccccaaaaac caccaacaag 120 aaaccaatca tggcagccgg ggcatggcca attctaggcc acctccatct gtttaaagat 180 ggggaattgc ctcaccacat gcttaaatcc atgtctgata aatacggccc tgccttcctc 240 atgaagttcg gccaacaccg aacactcgtt gtgagcaact accgcatggt aaaagaatgt 300 ttcactacta atgacaccca cttttgcaac cgtccatcca ccacagcctt cgatgtcatg 360 acttacgcta atgactccgt cgctttcaca gcttacagcc cttactggcg tgagcttaga 420 aagatttcca ctctcaaact tctctctaac aaccgtctcc aggccatcaa gaacctccga 480 gaagccgagg tgaacgtatc tttcaggggg ttgtatgatt tatggaagaa taatagtgat 540 cgagctgcgt cggttttggt cgatatgaag aaatggtttg aagaggtctc aaacaacgtc 600 gtgattaggg tgatcgtggg gaaacataat tttgggacta agattgtgaa gggtgagaag 660 gaggctgtgg aatacaagac gatcatggat gagctcttac gtcttgctag tttgtctttg 720 ttatctgatt ttgctcctat acttggttgg gtggacttct ttcaaggaaa cgttcgaaaa 780 atgaaacgaa atggcaagaa actagacgtg gtacttcaga ggtggttgga gacgcatcgg 840 aggaagaaga actcaccaga ggatgaacaa gacttcatgg atgttatgtt gtcgatcgtt 900 gaggagggcg acaagttgtc tggccaccac gctgatactg tcattaaagc tacttgcttg 960 gccatgataa tgggtgggac agacacgtcc gcagtgagtc taacatggat cgtctcctta 1020 ctgatgaaca atcgtcatgc attgaaaaag gctcgtgaag aattggatat gtacgttggg 1080 aaggataggc aagtagagga ttcagattta aaggacttgg tttacttgca tgcaatcgtt 1140 aaagaaacca tgcgactgta cccactgggt actcttcttg aacgcgagac caaagaagat 1200 tgtaaggttg gtgggttcca cgtcgaagcc ggtacgcgtt tactagtgaa catatggatg 1260 gtacaacgag acccaaccgt gtggagtgat ccaacaaaat ttataccaga gaggtttcta 1320 acagagaagg cggacataga tgttgggggt cagcatttcg aactcatacc attcggggcc 1380 ggtagacggg tgtgccctgc cgtgtccttt gcactccaat ttcttcactt gatactggct 1440 cgtctcatcc atggatatga attaggaacc ccaaatgacg cggatgtcga cttaaccgag 1500 agcccggaag gacatgttaa ccacaaagca tcccctctcg aactcctcct caccccacgc 1560 ctcgacccta agctctatat atga 1584 <210> 193 <211> 1608 <212> DNA <213> Sanguinaria canadensis <400> 193 atgaattcaa tcgcagtcgt cggtgttctt cttttcttcc tctactatct atggaggaca 60 ctattcacca ctcacaaagc ggcgccacca ccacaaccag ccggagcaag gcctattata 120 ggtcacctac atctcctcgg tggagcgaat caactagtcc atcaaacact cggttccatg 180 gcagacaagt acggatcaat tttcatgatc cgactcggga tgcgacgagt cctcgtggtg 240 agtagctccg agttagctaa agaatgtttc actacgaacg acaaggtttt cctcaaccgc 300 ccaagttcat tagctttaaa gctgatggcc tacaacaacg ccatctttgg ttttgcacct 360 tttggtcctt actggcgtaa gatgcgcaag atagcagtca ccgaacttct atccaacaga 420 cgcctcgaga tactaaaaaa catcaggatc tccgagatca acatgtctat aaaagaatta 480 catcaactat gggtagatat gaacagtggt gatgatcaag tggggggccc accaattgtg 540 gtggaaatga gaaagtggtt tggggattta tcgttcaacg tggtgggtag aatgatagct 600 gggaaaagat attttgggag gaattgtgat tgtgatgaag aagaaacgag gaggtatcag 660 aaggcgatgg gtgattttat gcatttagtg ggggtttttg tggtgtcgga tgcgattccg 720 ttattagggt ttttggattt acagggatat gagaagaaga tgaaaaggac ggccatggac 780 attgattgtg ttttagggag atgggttgat gaacatcgac gacgacgaca agaccacgat 840 gagaagagga ttatggtctc cgatgatgat catcaaggac aagatttcat tgatgtgatg 900 ctatcgatct taaatgatga tgatcagttc tacggttacg atgctgatac agttatcaag 960 tctacctgcc tgtctcttat cgcaggcggc cacgagacca cggcggtcac actaacatgg 1020 gccctctctt tactactaaa caatcgacac gtgttacaaa acgcgcaaga tgagttggac 1080 atccacatca gcaaggaaag acaagtggac gaatcagata tcaagaacct gaaatacctc 1140 caagccatag tcaaggaagc attgcgttta tacccagctt taccactttc agcactaaga 1200 ttggccatgg aggactgcac catagctgga tttcatgtcc ctaaaggcac agaattgatg 1260 cttaatctat ggaaattaca tcgagaccct cacgtttggt ctgacccttt ggagttcaag 1320 ccagaaaggt ttcttactaa tagcacccat gaagaagttg acgttggggg tcaacattac 1380 gaattactac catttgggtc tggtagacgg atgtgccctg gggtatcgtt tgctcttcaa 1440 gtcctgcact tgacgcttgc tcgtctactt catggatttc atttgtcgac tccgtccggt 1500 gtacctgtgg atatgactca aagttttggc cttagttgcc caaaagcaac tcccttggat 1560 gtcctcctta caccacgtct cccttcaaag ctatattatg tagcataa 1608 <210> 194 <211> 1806 <212> DNA <213> Sanguinaria canadensis <400> 194 atgatttcaa ctacgccgga cgtactagct acctcatcat attcatcatc taacaagcat 60 atggattcag atcatcttta ccggttctca actagtacta gtatagttat tggcctcttt 120 attttactat tcttccttgt gctcctatgg aaaccaaaga agcagtctgc tacttctaag 180 actaacaaac caactagaga gcagcgggca ccggaacctt ctagttcatg gcccataatc 240 ggtcatctcc gtctcttcgg aggaccaaat agccttcccc acataacatt gggagctatg 300 gctgataagt atggaccagt cttcaccatc cgaatgggtt cgcgtccagt gcttgttgtg 360 agtagttggg agacggcgaa agaatgtttc accaccaacg acagggtctt cgcctctcgt 420 ccacgcacta tagccattaa acatatgtgt tatgaacatg tcatgcttgg gttcgcccct 480 tacggatcat actggcggga attgcgcaag atagtgaacc gagagcttct ttcgaataat 540 cggctagagt tgttaaaaca tgtatgggga tctgagatta acacatccat taaagaactg 600 tatgagttgt ggttggtgaa caaaaacaac attgaagcag aaggtgaaaa tgaaggtaca 660 agtgataggg ttttggtgga gatggagaga tggtttgcgg atttgactct aaacatgtct 720 attaagattg ttgttgggaa gcgatacccc tccggtgtta ctgccggtgg tagttctggt 780 tgtgatgatg atgaagctca gaggtgccgg aaggccctga gagatttctt taagctggtg 840 ggtctgtttt tggtgtcgga tgcgatcccc tttctaaggt ggttggactt gggtggttat 900 gaaagagaga tgaagactac tgcaagagag ttggactgtt taatggagga atggttggaa 960 gaacataaaa tgaagaggag attattaatt tcaccgtcgg caggtgatca tcatgaagct 1020 gcagagatca tgatcaataa gcagaaggaa gcagagctac aggaatatga cttcatggac 1080 gtgatgctat ccatacttga tgacgatatt gagggtaaaa acctttcaga ttacgacgtc 1140 aatgccgaca ctatcaacaa gtctacttgc ctgaatctaa tcctaggtgg gagcgacaca 1200 acaatggtta ctttaacatg ggccctcgct ttactactaa ataatccaca cgtgttacaa 1260 aaggcccaag atgagttgga tatccacgta ggcagcaaga gacaagtgga cgattcagat 1320 atgaagaacc tcatatacct ccaagctata gttaaggaaa caatgcgcct atacccagca 1380 ggtccactat cagtaccaca cgaatcaact gaagactgca ccgtagctgg ctaccacgtc 1440 cctgcaggga cccgtcttat tgtaaatacc tggaagatcc aacgtgaccc acgggtatgg 1500 tccaacccat cagaattcaa accagagaga tttctcacag acagtcacgt ggacatagat 1560 gttagaggtc ggaattttga attgatccct tttgggtcgg gcaggaggtc gtgcccgggt 1620 acctcatttg cactccaagt ggtgcacctg gtactggccc gtttgataca tgggttcgag 1680 tttaaaacac cgtcggaagc acccattgat atgacggaga gcgtaggcct aagtaatttt 1740 aaagccaccc cacttgatgt tctgctcacc ccacgtctcc cttctcagct ttattatgtt 1800 tcttag 1806 <210> 195 <211> 1578 <212> DNA <213> Sanguinaria canadensis <400> 195 atggattcta ctttggtttt cattggcctc tttgctttac tactagtcta ccccttacta 60 ctaagaagat cagtattgaa gtctacttca aatactaata agactactta taaatcgaaa 120 aaccaagcac cggaggcggc cggggcgtgg ccgataatcg gtaatcttca tcaactcgcg 180 ggtggaggaa actgtctcca taaaacgttg ggagccatgg ctgacaagca cggaccggct 240 ttcaccatcc ggatgggtct acataaagca cttgtggtga gtagttggga gctagcgaaa 300 gagtgtttca ctaccaacga cgtggttttc atgtcccggc cgcatcaagt agccattaaa 360 cacatgggtt acagcaccgc catgtttgga atcgctcctt atggcccata ctatcgcgaa 420 atgagaaaga tagtgactca agagcttctc tctaaccgcc gcctagagtt gctaaaacat 480 gtatgggcct ctgaaatcaa caactccatt aaacaactct acgagaaagt cgaaggcgga 540 ccagttttga tcgaaatgaa gcgatggttt gcggatttaa cgctaagaac gacggttaag 600 gtgatttgtg gacagcaaca atttggagga gatgatgatg atgatgaagc tggaaggttt 660 caaacggcgc tgagagactt ctttaggttg ttaggtcact ttcgagtggc ggatgtgata 720 cctgttctag agtggctcga ttttcaggga tacaagaaag agatggagaa taatggaaga 780 gtgcttgata atttaatgaa taaatggttg gaagaacata aaaggaagag gaagaacggt 840 ggcaccgaag aggacttcat gaacgtgatg atatccaaac tgcttgatga tacaaagatg 900 ctttcctatt acgacgccga tactatcaac aaatctactt gcctgacact aatcctgggt 960 gggagtgaca caaccatggt tagtttaacg tgggcccttt ctttactagt gaatcatcca 1020 catgtgttga aaaaggccca agaagagttg gatgtccacg taggcaaaga aagacaggta 1080 gacgattcag atctctggag cctcacatac cttcaggcta ttatcaagga aacattgcga 1140 ctatacccac ctggtccact ctcagctcca cgcgaatcaa cggacaactg caccatagct 1200 ggctaccacg tccctgcagg aacccgtcta atagtgaata cctggaagat tcaacgtgac 1260 ccacgggtat ggcccgaccc attagagttc aagccagaga gatttctcac cacccatgtg 1320 gacgtggacg ttaaaggtca gaatttcgaa ctcctaccat tcgggtcggg tagaagagca 1380 tgcccaggtg cctcattcgc gcttcgggtg ctgcgcctgt cactggctcg tttgatacat 1440 gggttcgact ttaaaacacc attggacgac tcacccattg atatggtgga gagtggagga 1500 ataactaatg ttaaagacac cccacttgaa gtactagtca ccccacgcct cccttctagt 1560 ctttatgttt gcaaatag 1578 <210> 196 <211> 1599 <212> DNA <213> Sanguinaria canadensis <400> 196 atggattttg atctttacca attctcaaaa cctactatct tcattggagg cctctttgct 60 atacttctct actttgtact caaaaaatct agtactccca aatccaaatc aaaattagaa 120 caaccacccg aaccggctgg cgcgtggccg atcatcggtc atcttcctct cctcggagga 180 cccgatctcc ctcacataac attaggaaaa ttggctgata aatatggacc agccttcacc 240 atccgaatcg gtgttcataa agcggtggtt attaatagtt gggaggtggc gaaagagtgt 300 ttcaccacca acgacaaggc gttttcttcc cgtccgcgtc aagtagccat gaaacacatg 360 ggttacgatt acgccatgtt tgggttcgcc ccttacggaa attactggcg tgaactccgt 420 aaaattgtga accgagaggt tctgtctcat agtcggatcg agtctttata tcacgtatgg 480 ggtactgaga tcaacacgtc cgttaaagaa ctgtacgagt tgtgggggaa gaaaagcacc 540 ggcagcggcg gtgcaccggt cttggtggaa atgaaacgat ggttctccga catgacactc 600 aacatgtctg ttatgatggt tgcaggtaag agatacaact ttagtggcaa caaagctgac 660 gatgaagctg ggaggtgcca agatgggctg aggaacttct ttcggttagt cggtctgttc 720 gtgccatccg acgcgttgcc gtttttggcg tggttggaca ttggtgggta cgagaaagaa 780 atgaagaaag tagcgaaaga gcttgatgtt ttagtgcagg aatggttgga agaacacaaa 840 gagaagaggt tggcattgac ggccgcgggg aagaaaggaa gcgaaaacga tttcatggac 900 gtgatgatga atatacttga agatcaaaaa ctatcagaat tcgatgcaga tactatcaac 960 aaggctactt gtctgacttt aatcctcggt ggaaccgata caaatatggt taacttagtg 1020 tgggccctga ctttactggt taacaatcaa gatgcgttga agaaagctca tgatgagttg 1080 gacttccacg taggcaaaga tagacaagtt gaagaatcag atgtaaagaa cctcgtgtac 1140 atccaggcta taatgaagga aacactgcgt ctatactcgg gcccactgtc gggcttacgc 1200 gaatctacgg aggactgcac cgtagctggt tactacgtcc cagcagggac ccgtttgatt 1260 attaatgcag cgaagattca tcgtgaccca cgggtatggt ctgacccaac agctttcaaa 1320 ccggatagat tcctcacgga acagaaagaa atggatgtta gaggtcagga tttcgaaatc 1380 cttccattcg gggcgggtag aagaatatgc ccgggtgtgt cattcgcact tcaagtattg 1440 ccattggcac tggctcgatt aatacatggg ttcgacttta aaacacccac ggatgcaccc 1500 attgatatga cagaaagtcc aggactaact aatgctaaat ccaccccact cgaagttcta 1560 gtctcacccc gcctcacttc taaactttat ggttgttaa 1599 <210> 197 <211> 1668 <212> DNA <213> Sanguinaria canadensis <400> 197 atggattcta ttctaaacca gttcatctca aaacctacta tggttatttg cagcctcttt 60 gctttactat ccctctactt tctactgatc aaaaggtcta ccactaaatc aaaattacaa 120 ctgccacccg agccggcggg tgcgtggcct gtcatcggtc atctccatca gttgggtgga 180 cctaacttac cccacataac attagcagcc atggctgata agtatggacc aatcttcacc 240 atgaaaattg gtacgtatcg agcactcgta gtgagtagtc cggaggtagc gaaagagatt 300 ttcaccaccc acgataggat atgggcaacc cgaccaaatc aagcagccat gaaacacttg 360 ggttacgact cggccatgtt tgggttcgca ccttatggac catactggcg tgaacttcgc 420 aagttagtga accgagaact tctctctaat actcggctcg atttgctaca tcatgtgtgg 480 gattctgaga ttaacacttc cattaaagaa ctgtacgatt cgttagcgac caaaaacaat 540 gcaaaaggcc gtggtactgg cggcgccggt agtactggtc ctgttttggt ggaaatgaaa 600 cggtggttcg cggatttgac gctaaacata actgttagga tggttgctgg gaaacgatac 660 tttggtgcta ataaaacttc tagtactagt actgatcaat gtgataatga aaaaggagat 720 agtaaagcat ggaagtgcca aagggagctg agaaacttct ttaggttggt aggtctgttt 780 gtagtgtcgg acgcgttacc gtttcttggg tggttggatt tgggtggtca cgagagagag 840 atgaagaata ctgcaagaga gttggatgtt ttagttcaag gatggttgga tgaacataag 900 aggaagagat cattatccgt ggctgaaggc ggggagaaga tcaacggcga acaggacttc 960 atggacatga tgctgtccac aatcgacgac gctaagttat ctggttattt cgacgctgat 1020 actatcaaca agtctacttg tctgaatcta atcctgggtg ggagtgacac aatgatggtt 1080 aatctaattt ggcccctcac gttactggtg aactatccag acgagttgaa gaaggtttat 1140 gatgagctgg acacccacgt aggcagagat agacaagtgg acgaatcaga tatcaagaat 1200 ctcgtgcacc tcaaggctgt aatcaaggaa acaatgcgtc tatacacggg tcggatcaca 1260 ggcctccgcc aatcatcaga ggattgtacc gtggcagggt accacgtacc agcaggtacc 1320 cgcctgattg taaacacgtg gaagattagc cgagacccac ggtactggtc cgacccgtta 1380 gagttcaagc cagagagatt tctcacgacc catgcggata tcgagcttac aggtcagaat 1440 tttgaactaa tcccgtttgg gtcgggcaga agatcatgcc cgggtgcctc atttgcaatt 1500 caattgctgc acctggcact ggctcgtctg gtacatgggt tctggtttga aaggccgtcg 1560 gatgcaccca ttgatacatc agagtgtcct ggactaacta attttaaagc caccccactt 1620 gaagttctgg ccaccccacg cctcccttct aaactctatg ttggttaa 1668 <210> 198 <211> 1617 <212> DNA <213> Sanguinaria canadensis <400> 198 atggatttta ttctgaacca gttcatctca aaacctacta tggttcttgg caccctcttt 60 gcattactat ccctctactt tctactgatc aaaaggtcta ccactaaatc aaaattacaa 120 ctaccacccg aaccggctgg tgcgttgccc atcatcggtc atctccatct cctcggtgga 180 ccaaacccgc cccatataac attagcaaac atggctgaga agtacggacc gatcttcagt 240 atcaagatgg gtttaaaacg agcactcata gtgagtagtt cggaagtaac caaggagtgt 300 ttcactaccc atgacttgaa tttcgtctct cgaccacgtc atgcggctat gaaactcatg 360 ggttataatt tcgctacgtt tgggttcgcg ccttatggac cttattggcg cgaacttcgt 420 atgataatta accgagaact tctctctcaa actcggatct tgtcgttaga acatgtatgg 480 ggttctgaga tcaatgaatc cattaaggaa ctgtatgatt tgttgacgag aaaaggtggt 540 ggtggtggtg gtccggtggt ggtggaaatg aagcgatggt ttgcggattt gaccttaaat 600 atagccgtta agatggtgtg tgggaagcga tacttcggtg ttaattctcc tagcactagt 660 acgactcgtg atgttcaaga agatgatgaa gctcggaggt gccaaaaggg gatgagaaat 720 ttctttaggt tgcttggtca gtttgtggtg tccgactcga taccgtttct ggggtggttg 780 gatttgggtg gttaccagag ggagatgaag aataccgcca gagagctcga cgatttaatg 840 cagggatggc tggaagaaca taagaagaag agattggaaa ggaagacggc cgcaggggaa 900 caagacttca tggacgtgat gctgaacata cttgaagatg gaaagctatc tcaatatttc 960 gacactgata ctatcaacaa gtctacttgc ctgagtctaa tcctgggcgg gagtgacaca 1020 acgatggtta atttagtgtg ggccttcagt ttactggtga aacatcaaga tgcgttgaag 1080 aaaactcgtg atgagttgga catccacgta ggcagagaaa gacaagtgga ggaatcagat 1140 atcaagaatc tggtattcct ccaggctgta gtcaaggaaa catttcgtct atacgctggc 1200 ccaatctcag gagtacggga agcagcggag gactgcacca tagcaggcta ccacgtccca 1260 gcagggaccc gtctacttat aaatagctgg aagattcacc gtgacccacg ggtatggtcc 1320 gacccattag agttccaccc agaaagatat ctcgaggcga ggcatgcgga cattgacgtt 1380 aaaggtcaga atttcgaact cgccccattc ggaatgggtc gaagagtttg cccgggttcc 1440 gcatttggac tgcaagtatt gcacctgaca ctggcccgtt tgatacatgg gttcgagttt 1500 aaaacaccgt cagacgcacc cgtggatatg acggagagtg taggaatgag tattattaaa 1560 gccaccccac ttgaagttct cgtcacacca cgcctccctt ctgaactata cgtctaa 1617 <210> 199 <211> 1602 <212> DNA <213> Sanguinaria canadensis <400> 199 atggatattt ctaatcttta cgagttatac tatttctcca cacctactct gtttgtcgga 60 ggcctctttg ttttattact catctactct ctattctcat ccaggcctgg aattaaatca 120 aaattacaac agccacctga accggccggt gcatggcctc tcatcggtca tcttcctgtc 180 ttaagtggac cggaactacc ccatgtcgca ttgggaaagt tggctgacaa gtacggacca 240 gtcttcacca ttcgtatggg tgttcataaa tcacttgttg ttagtgattg ggaggtaatt 300 aaagagtgtt tcactaccca cgacaaggtc ttctcgtctc gtccttgtca agttgctatg 360 aaactcatgg gtacggccat gtttggcttc tccccttacg gaaattattg gcgtgaactt 420 cgcaagatca tgaacagaga ggttctatct catggtcgga tcgaatctct ttaccatgta 480 tggggttctg agatcaatac gtccgttaaa gaactttacg atttgtgggc gaagaaaaac 540 aatggtggtg gtccaatttt ggtggaaatg aagcgatggt tctccgattt aacgcttaac 600 atgtcggtta tgatggccgc tggaaagcga tataattttg gtgatgattc ttctactact 660 aatgatgaag cggcgaggtg ccaacatgcg ctgagagagt tttttaggtt ggtgggtttg 720 tttgtgccat cggacgcctt accgtatctt gggtggttgg acataggtgg gtaccaaaaa 780 caaatgaaga aagtagcaag agaattggat gatttaatgc aggtatggtt ggatgaacat 840 aaaaagaaga ggttagcagc gaaagccgaa gggaagaagg gaggcgaaca agacttcatg 900 gacgtgatga tgactatact tgaggacgga aagctatctg attattacga cgctgatact 960 atcaacaagg ctacttgctt gactctaatc ctgggtggga ccgacacaaa catgcttagt 1020 ttagtgtgga ccctggcttt actgatgaat aatcgacaag ccttaaagaa ggttcatgaa 1080 gagttggata tccacgtagg cagggaaaga caagtggaag aatcagatat caagaacctc 1140 gtatacctcc aggctgtaat caaggaagaa atgcgtctat actcgggccc actttcaggt 1200 ctacgcgaaa caacggagga ctgcaccata gctgggtacc acatcccagc ggggacccgt 1260 ttgattataa atgcatcgaa gctccaccgt gatcctaagg tatggtccga cccgttagag 1320 ttcaagccag agagatttct tacggaaaac gtgggcgtgg atgttagagg tcagaatttg 1380 gaactccaac cattcggggc gggtagaaga atctgcccgg gtgtctcatt tgcacttcaa 1440 gttttgcccc tgacgttggc ccgtttgata catgggttcg agttgaatac acctggggat 1500 gcacccattg atatgacgga gtctccagga ctacaaaatg ctaaactcac cccacttgaa 1560 gttttaatca caccccgact cccttctaaa ctatatgttt aa 1602 <210> 200 <211> 1608 <212> DNA <213> Sanguinaria canadensis <400> 200 atgattgtta ttggcgcctt ccttgcttta gtggtactag tggtctacta taatctacta 60 ctaagaaggc cagcattatt gaaatttaaa aacatgaagc agcaggcacc agaagcggcc 120 agtgcgtggc caataattgg tcatctccat aatctcgcgg gtggcatagg cggaaacggc 180 ctactccacg aaaagttggg agcaatggct gacaagcacg gaccggcttt catcattcgc 240 ttggggctgc ataaagcact ggtggttagt agttgggagg tagtgaaaga gtgtttcact 300 acaaacgacg ttgccctcat ttcccgcccg catcaagtag cttctaaaca catgggttac 360 ggcatggcca tgtttgcgtt cgcctcgcct tatggaccat actggcgcga attgcgtaag 420 attgtgaagc aagaacttct ctctaatagc cggcttgagt tgctaaaaca tgtatgggcg 480 tccgagatca acacctctat taaacaactc tacgagaaag ttaaagaagg tggtggtttg 540 ccggttttgg ttgaaatgaa gggatggttc gcggatttaa ccctaaaaat gacggtaaag 600 gtgatttgtg ggaagcgaca ctttcgagtt gcgggtaatg taggagatca tgttgatgct 660 gatgacgatg atgaagtaat tggtgggcga tttgaaaagg cgctgagaga cttttttagg 720 ttgttaggag atattcgagt ggtggacgtg ttaccgtttc ttcggtggtt ggattttggc 780 gttggttata agaaagagat ggagaataat ggaagagtgc tggatatttt aatggaggaa 840 tggttgcaag aacataaaag gaagaggatg aacggcggca ccgaagagga cttcatgaac 900 gtgatgatat ccaaattact taatgataca aagctgcttt cctattacga tgctgatact 960 atcaacaaat ctacttgcct gactttaatc ctgggtgcca gcgacacaac tatggttact 1020 ttaacgtggg ccctctcttt actagtgaat catccacaag tgctaaaaag ggcccaggac 1080 gagttggatg tccacgtagg cagggaaaga caggtggaag attcagatat ccagaacctc 1140 acataccttc aggctataat caaggaaaca ttgcgaatat gcccaccggg tccgattcaa 1200 gctccacacg aagccaagga cgactgcacc gtagctggct accacgtccc tgcagggacc 1260 cgtcttatag taaatacctg gaagatccaa cgtgacccac gggtatggcc caacccgtca 1320 gagttcaagc cagagagatt tctcacgacc catgtgggcg tggacgttag aggtcataat 1380 ttcgaactca tacctttcgg gtcgggtaga agatcgtgcc cagggacctc gttcgcactc 1440 caagttgtgc atctgacact ggctcgtttc atacatggat ttgagtttaa aacaccgtcg 1500 gacgcaccca ttgatatgac ggagagtgga cgaataacta atgttaaagc caccccactt 1560 gaagttctag tcaccccacg catcccgccg tctggtcttt atgtttaa 1608 <210> 201 <211> 1719 <212> DNA <213> Sanguinaria canadensis <400> 201 atggatttgc tgatcttctc cagccagttg caaggtactg tggggttatt agctttacta 60 accttttcct attatgtatg gattttaatt ataaagagga tcaaaacaac caacaccttg 120 aaggtcatga ataaagtggc ggccgcggcg gcaccggaag ttgccggtgc atggcccata 180 gtgggtcatc ttcctcagct agttggccct caacagcttt acaggattct tggagacatg 240 gccgacgagt atggaccgat cttcatggtc cggtttggga tgtacccaac tctggttgtg 300 agcagttggg agatgataaa ggagtgcttc actaccaacg acaggttcct cgctagtcgt 360 ccctctagtg ctgctgggaa gtacctcact tacgacttcg ctatgttcgg cttctccttt 420 tatggtccct attggcgtga gatccgtaag atctctactc tcgaactact ctcccatcga 480 cgccttgagt tgtttaagaa cgtccctttc actgagatcg acacctgcat caaaagattg 540 taccaacttt ggcgggttaa aaataatgat catccagctg ctgctccggt aattaaggtc 600 gacatgagcc aattgttgag agatctaacc ttaaatacaa tattgaagtt ggtggtagga 660 aagaatttat tcaacgagaa ggatcatggt gaacaggaag agggtcgaaa gctccacctc 720 cacaagacga tagttgaatt ctttaaactg gcaggggttt cagttgcatc ggatgctctt 780 ccgttcctag ggtggctgga tctggatgga cagaagaaaa agatgaagag gatagccaag 840 gaaatagact taatcgctga aagatggctt caagagcatc aagagaagaa atcactatca 900 aacaagaagc tgctggctgc tggcggcggc aaagtagacg gtcatgacga cttcatggac 960 gtgttgttat tcattcttga cgatgattcc cagttcttca atttcagtcg cgacactgtc 1020 attaaagcca cctcttgggc aatgatccta acagccgcgg acaccacatc cgtgtctatg 1080 acatgggctc tgacgctact actcaccaat ccccgtgtct taagaaaggc ccaagatgag 1140 ctggatatga aagtcggcag agacagacac gtggaagaac gagatatcga gaacctgatc 1200 tacctccaag ccatcgtcaa ggaaacattg cgtttgtacc cggctgctcc acttggagta 1260 cctcacgagg ctatccaaga ctgcaccgta gctgggtatc aggtaagagc aggcacacgt 1320 gtgttagtga acctatggaa gctgcatcgc gacccacgtg tgtggtcgaa cccatccgaa 1380 ttcaggccgg agaggtttct aatcgacgat attgacgagc aaggtggtgg tggtggtggt 1440 ggtggcggtg aatcaacagc taaggatttc agaggtcaac attttgagta cataccattt 1500 gggtcaggac gaaggatgtg tcccgggatc aacttcgccc ttcagatcgt tcacatgaca 1560 ctggcacgcc tacttcatgc attcgagttg aggactacta cgtccgcatc actaatggac 1620 atgaccgaag aatcaggcct caccatgccc aagaaaaccc cacttgtagt cctcctccaa 1680 ccacgcctcc ctcttccact ttatgatcac catgagtga 1719 <210> 202 <211> 1602 <212> DNA <213> Stylophorum diphyllum <400> 202 atggagtttc tctctctaca acaccaacta atttccattt ttgctcttct tcttgcttcc 60 atattcctct acaatctctt gaagaatcat ggaaggaaat ccaagacctc aaaaccacca 120 gcacctgaag catcaggtgg atggccaatc atgggtcatc tcaatctctt caatggaagc 180 gaattaactc accaagctct aggttccatg gctgacaaat acggtccagc tttcaatatc 240 cgattcggtt ctcatcaaac actcgttgtc agtagctcgg agattgttaa agaatgtttc 300 actacaaacg atcgattctt ttcaaatcgg ccgggatcgt tagcgattaa actcatgttc 360 tacaacgctg attcagttgg ttacgcaccg tacggtgctt attggagaga tctcagaaaa 420 atctcaacct taaaactcct ttcgaatcat cgactggaaa ccctaaaaca tttgagaact 480 tctgaagttg agtcctgttt taaacaactt tataatcaat ggaagaacaa caaagttggc 540 ggcgaccatg attttgctct ggtgaggatg gataattggt ttggagactt gacatttaac 600 gttgtggcaa gaattgtcgc tggaaaaaag aattttgctg gaggtgcgac gaatggcgac 660 gtcggagctc agagatataa ggaagctatg gatgaagcgt tccggttgat gacgattttt 720 gcgttctcag atgtggttcc gtctttggga tggttggata aattgagagg tttggttgga 780 gggatgaagc gttgtggtgc ggaaattgat tccattgttg cgggttgggt ggatgaacat 840 agattgaaga gagcttctgg aaagggagga ggtcatactg atcttgaaca agattttatt 900 gatgtttgct tggagattat ggaacattct actttgcctg gtgatgatcc tgaaattgtc 960 atcaaatcta cttgtctgga catgattttg ggtgggagtg acacaacaac ggtgacccta 1020 acatgggcac tctccttact attgaacaat cctcacgtgt tgaaaagggc tagggaggaa 1080 ttggatacac atgtgggaaa ggacagacaa gtagacgact ccgatatgtc taatcttgtg 1140 tacatccagg cgatcatcaa agagacgatg agattgtacc cagcaggacc actgatcgag 1200 cgaaggacat cggaggattg tgaggtaggt gggttccatg taccggcggg cacacgctta 1260 ttggtgaact tatggaagat gcaacgagat gggagtgtgt ataaggagga tccattagag 1320 tttagaccag agagattctt gactagcaat gctgatgttg atctaaaggg acagaattat 1380 gaactgatac catttggtgc gggtaggagg atatgtcccg gtgtgtcgtt tgcggtgcag 1440 ttaatgcatt tggtgctggc tcgtctcgtt catggctttg aaatgaagac ggccggagat 1500 gcgaaggttg acatgacaga aagtgcagga ctaattagcc acaaggttac accgctagaa 1560 gtgctgctca aaccatgcct tgcgattcaa caagctttat aa 1602 <210> 203 <211> 1602 <212> DNA <213> Stylophorum diphyllum <400> 203 atggattctt ttcttctaat ccagtggttt gcagcttcca tggctgctct acttgctttt 60 gtttttctct acaatcttgt ctggagttct tcaagaacta caaagggtaa gaatattaga 120 aaggcaccca tggcggccgg tgcatggccg attcttggtc acctacatct gtttggatcc 180 ggtgagctgc ctcacaaaat gctctctacc atggctgaga agtacggtcc cgctttcacc 240 atgaagtttg gtaagcacac aacactagtt gtgagtgaca cccgtatcgt aaaagaatgt 300 tttactaccc acgataccct ctttgctaac cgtccttcga ctactgcatt tgatctcatg 360 acttatgcta atgactcggt agccttcaca ccttatggtc cttattggag ggaacttagg 420 aagatatcca ccctcaagct tctctctaac caccgcctcc aatctatcaa ggaaattcga 480 gtatcagagg tgaacgtatg ttttagggag ctatatgaat catgcaagag taaaactgat 540 gcaactccgg ttttggtgga tatgaagaaa tggttcgaag aggtctcgaa caacatagtc 600 atgagggtaa tagtggggag acagaatttt ggttctaaga ttgtgcaagg cgaagacgag 660 gccgtcaatt acaagaaagt aatggatgaa ctcttacgtc tcgctagttt gtctatgtta 720 tccgatgtgg cccctgtact tggctggttg gatatgttcc aaggaaacaa gagcgccatg 780 aaacgaaatg ccaaaaaagt agacaccata cttgagggct ggttggagga gcataggatg 840 aagaagagcg catcaggaat ctcttctgct ggtgagaatg accaggactt catggatgtt 900 atgttgtcca tcattgagga gaccaagttg tctggccgcg atgcggatac cgttattaaa 960 gctacttgct tggccatgat catgggtggt acagacacca ctgccgtgag tctaacatgg 1020 attgtctctt tactaatgaa ccatcgccat gtactgaaga aggcccgaga agaattggac 1080 gccctcgttg gtaaggatag acaagttgaa gattcagatt tgaagaactt ggtatacatg 1140 aatgccatag tcaaagaaac gatgcgaatg tacccactgg gtgctatgct cgagcgagaa 1200 accaaggagg attgtgaggt tggtgggttc caagtccaag gcggcacacg attactagtg 1260 aatgtgtgga agctacaacg agacccaaac gtttggaccg atccaacaga attcaaacca 1320 gagagatttc taaacgaaaa tgcggatata gacgttgggg gtcaacattt cgagctacta 1380 ccatttggag ctggtaggag ggcgtgcccg ggtgtgtcgt tcgcactcca attcatgcat 1440 ttggtactgg ctcgtctcat ccatggatat gaattgggaa ctcagaatga tgcggacgtt 1500 gacttaactg agagcacaga aggacatgtt aaccacaaag cttcccctct cgatctcctc 1560 cttaccccac gcctcaacaa ccctaatctg tatgattatt ag 1602 <210> 204 <211> 1707 <212> DNA <213> Stylophorum diphyllum <400> 204 atgactatag gggctctagc tttactatct ttcatctatt ttttacgggt ttcagttatt 60 aagaggacca aatacaccaa caccgcggtc accgccacca acaagttgga aaatgatgaa 120 gatgaggcca atcatagtaa gcgagtggtt gcaccacccg aagtcgccgg tgcatggcca 180 atattgggtc atcttcctca gctagtggga ctaaagcagc cactgttcag ggtactagga 240 gacatggccg acaagtatgg acctatcttc attgtccgat ttgggatgta ccctacccta 300 gttgtgagca gttgggagat ggcaaaggag tgcttcacca ccaacgacag ggtcctcgct 360 agtcgtccag ctagtgcttc tggcaagtac ctcacctaca attatgccat gttcggcttc 420 actaatggcc cttattggcg tgagatccgt aagatctcta tgctcgaact gctatcccat 480 cgtcgcgtag agttgctcaa gcacgtacct tccactgaga ttgacagctc tatcaaacag 540 ttgtaccatc tttgggtgga aaaccaaaac caaaataagc aaggggatca tcaggtaaag 600 gttgacatga gccaactact cagagatctg accttgaata tagtattgaa gttggtggta 660 ggaaagcgtc ttttcaacaa caacgacatg gatcatgaac aggatgaagc agctcgtaag 720 ctccagaaga caatggttga actcattaaa gtggcagggg cttcagttgc atcggatgcc 780 cttccattcc ttggttggtt ggatgtagat ggactaaaga gaaccatgaa gaggatagcc 840 aaggaaatag atgtaatcgc tgaaagatgg cttcaagagc atcgacaaaa aaaactaaca 900 tcaaacgaca agggtggcag caacaacatt caaggtggcg gtggcgataa cgacttcatg 960 gacgtgatgc tatccatcct tgacgatgat tccaatttct tcatcaatta caaccgcgat 1020 actgtcatta aagccacctc tctgacaatg atattagcag gttcggacac cacaactctg 1080 tccttgacat gggctctaac gctattagcc accaaccccg gtgccttgag aaaggctcaa 1140 gatgaacttg atactaaggt gggcagggat agacaagtag atgagcgaga catcaagaac 1200 ctggtctacc tccaagccat tgtcaaggaa acactacgga tgtacccagc tgctccacta 1260 gcaatacccc acgaggctac ccaagattgt attgttggcg ggtatcacgt aacagcaggc 1320 acacgtgtgt gggtgaatct gtggaagttg caacgggatc cacacgcgtg gccaaaccca 1380 tcggagttca ggccagagag gtttctagcc gttgagaacg attgcaagca acagggtact 1440 tgtgatggtg aagcagcaaa catggatttc agaggtcaac actttgagta catgcctttt 1500 gggtcaggaa gacggatgtg tccaggaatc aactttgcga tccagatcat tcacatgaca 1560 ctggcacggc tacttcattc attcgagttg cgggtgccag aagaagaagt aatcgacatg 1620 gctgaagatt caggtctaac catatccaaa gtaacaccac ttgaactcct acttacccca 1680 cgcctccctc ttccacttta tatatga 1707 <210> 205 <211> 1584 <212> DNA <213> Stylophorum diphyllum <400> 205 atggctgccc ttcttccctt agctttcctc tacaatatct ttttggcttc atcttcgaaa 60 gctactagca agagaacaat aagtactaag aaaccgccca tggtagccgg tgcatggcca 120 attcttggtc atctccatct gtttaaagag ggtgagttgc ctcaccaaat gcttaaatcc 180 atggctgaca agtatggtcc agccttcctc atgaagttcg gtcaacacca atccctagtt 240 gtgagtgact accgcatcgt aaaagaatgc tttactacta atgataccct cttctgcaac 300 cgtccatcca ctacagcttt cgatgtcatg acttacgcta atgaatcggt agctttcaca 360 gaatacagtc cttactggcg cgagcttaga aagatatcca ctctcaagct tctctctaac 420 aaccgtctcc aggccatcaa gaacctccga gaagaggagg tggatgtgag cttcaagggg 480 ttgtatgatt catggaagaa taagaacaac aagagtactg ctggcagtgt tggtgatgaa 540 cgagctccgg ttttggtcga catgaagaaa tggtttgaag aagtgtcaaa caacgtggtg 600 attagggtaa tcgtgggcaa atgtaatttt gggactaaga tcgtgcaagg tgagaaggag 660 ggtgtggagt acaagacaat catggatgag cttttacgac ttgctagttt gtctttgtta 720 tccgatttcg cccctatact tggtttgttg gatttcttcc aaggccatgt ccgtaccatg 780 aaacgaaatg gcaagaaact agacgtgtta cttcagaggt ggttggagga gcataagagg 840 aagaagagca caccagagga tgagcaagac ttcatggatg ttatgctgtc gattattgag 900 gagagcaagc tgtctggcta tgacgctgat accgtcatta aagctacttg tctggccatg 960 atcatgggtg gaacagacac atccgcagtg agtctaacat ggatcgtctc tttactgatg 1020 aacaatcgtc aagcactaaa aaaggctcga gaagaattgg acgcgcaggt ggggaaggat 1080 agacaagttg aagattcaga tttaaagaac ttggtttact tgaatgccat cgttaaggaa 1140 acaatgcgat tatacccact aggtactctt cttgaacgcg aaactaagga ggactgtgaa 1200 gttggtgggt ttcacctcga aggtggtact cgtttactag tgaacgtatg gatggtacag 1260 cgagacccag acgtgtggac tgatccgaca aagttcatac cagagaggtt tcttacggag 1320 aaggcagaca tagatgttgg gggtgggaat tttgaactta taccattcgg agccggtaga 1380 agggtgtgcc ccggagtgtc ctttgcactt caattcctaa atttggtact agctcgtctc 1440 attcatggat atgaattggg aactccagaa gatgcggatg tggatctaac tgagagccca 1500 gaaggacatg tcaaccacaa agcatcccct ctcgagcttc tcctcacccc acgcctcagt 1560 aaccctaagc tctatgatta ttag 1584 <210> 206 <211> 1644 <212> DNA <213> Stylophorum diphyllum <400> 206 atggattctg atcatcaact tctcagttac cagttctctg caagtaatat gtttatcagc 60 ctctttgctt ttgttctcta ctatctacta gtatggaggc caacaaaatc taatcttaag 120 atgaaaacca tatcatctga agacaagcaa gcacccgaac tcgccggttc atggccagtt 180 atcggtcatc ttcatctcct tcatggacct aacccactcc atgtagcttt gggagccatg 240 gcggacaagt acggaccagc tttcaccgtt cgagttggtg tccatcggac acttgttgtg 300 agtagttggg aggtagcgaa agaatgtttc actaccaatg acaaggtctt tgtatctcgt 360 ccacgtcaag cagcaggaaa acacatgggt tacaacaacg ccatgttgcg attcgcctcc 420 gatacaccat actggaccca aatccgtaaa atgttgaaac gagacctcct ctctaacaac 480 cggattgagt tgctaaacca tgcatggcat tccgagatta acacatccat taaagaactg 540 tacgagatga attggtcatc aggcgaagga cgtcgtggcg gcattagacc gccggtctcg 600 gtggacatga agcaatggct tggtgattta accctaaaca tgtctgttaa aatgattgct 660 gggaagagat gttttggcag tggtgtttct gcttgtgatg aaggagaagc tagaaggtgt 720 caaaaggggt tgaaagactt cgttaggttg atgggtcaga ttgtggtgtc ggatgcaata 780 ccatttcttg gatggttgga tttggatggc tacgaggggg aaatgaagcg agccggaaaa 840 gagctcgacc gtttactagg aggatggttg gaagagcata aaatgaagag atcaccaggt 900 gatgaagctt cagcccagaa ggactggatg gacttgatgc tgtccgtact tggggatgga 960 aagcttgacg gctattacga cgctgatacg atcaacaaag ctacttgtct ggctctattg 1020 cagggtggta gcatctggac gatgcttact ttattatggg catttgcctt actagtgaat 1080 catccacatg tgatgaaaaa ggcccatgat gagctggaca accatgtagg cagagaaaga 1140 caagttgagg aatcagacat caagaacctc acttaccttc aagccatagt caaggaagca 1200 atgcgtcttt actcggtgtc gatgcgacta ctcgaatcca ccgcagactg cactgtttct 1260 ggctacgatg tcccagctgg gactcgtcta atcgtaaata cttggaagat tcagcgtgac 1320 cctcgggtat ggtccgaccc atctgaattc catccagaga gattcctcac acaccgccat 1380 agggacatgg acttgtatgg tcagaatttt gaaatcacac cttttgggtc gggcagaaga 1440 tcatgcccag gttcctcatt gggtcttcaa atggtgcaac tgacaatagc tcgtttgcta 1500 catgggtttg agtttaaaac accttcggac gcccccattg atatgactga gagtgttggg 1560 gtagaaaata tggttaaagc aacaccaatt gaagttctgc tcaccccgcg cctcctcgat 1620 gaggtttatg ctttctataa ttag 1644 <210> 207 <211> 1687 <212> DNA <213> Stylophorum diphyllum <400> 207 cttttgccag ttccaaggta ttgtaggaat cttattagct ttcctaacct tcttatatta 60 tctatggcgg gcttcaatta caggattaag gactaaaccc aaacacaacg actttaaagt 120 cactaaggca gcacctgaag ctgatggtgc atggcccata gttggtcatt ttgctcagtt 180 cataggccct cggccacttt tcaggattct tggagatatg gccgacaagt acggttcgat 240 cttcatggtc cgatttggga tgtacccaac cttggttgtg agcagttggg agatggcaaa 300 ggagtgcttc actaccaacg acaggttcct tgctagtcgt ccagctagcg ctgctggcaa 360 gtaccttacc tacgactttg ctatgttaag tttctccttt tatggccctt attggcgtga 420 gatccgtaag atctctatgc tcgaactgct atcccatcgt cgcgtagagt tgctcaagca 480 cgtaccttcc actgagattg acagctctat caaacagttg taccatcttt gggtggaaaa 540 ccaaaaccaa aataagcaag gggatcatca ggtaaaggtt gacatgagcc aactactcag 600 agatctgacc ttgaatatag tattgaagtt ggtggtagga aagcgtcttt tcaacaacaa 660 cgacatggat catgaacagg atgaagcagc tcgtaagctc cagaagacaa tggttgaact 720 cattaaagtg gcaggggctt cagttgcatc ggatgccctt ccattccttg gttggttgga 780 tgtagatgga ctaaagagaa ccatgaagag gatagccaag gaaatagatg taatcgctga 840 aagatggctt caagagcatc gacaaaaaaa actaacatca aacgacaagg gtggcagcaa 900 caacattcaa ggtggcggtg gcgataacga cttcatggac gtgatgctat ccatccttga 960 cgatgattcc aatttcttca tcaattacaa ccgcgatact gtcattaaag ccacctctct 1020 gacaatgata ttagcaggtt cggacaccac aactctgtcc ttgacatggg ctctaacgct 1080 attagccacc tawcccctgt gtgccttgag aaaggctcaa gatgaacttg atactaaggt 1140 gggcagggat agacaagtag atgagcgaga catcaagaac ctggtctacc tccaagccat 1200 tgtcaaggaa acactacgga tgtacccagc tgctccacta gcaatacccc acgaggctac 1260 ccaagattgt attgttggcg ggtatcacgt aacagcaggc acacgtgtgt gggtgaatct 1320 gtggaagttg caacgggatc cacacgcgtg gccaaaccca tcggagttca ggccagagag 1380 gtttctagcc gttgagaacg attgcaagca acagggtact tgtgatggtg aagcagcaaa 1440 catggatttc agaggtcaac actttgagta catgcctttt gggtcaggaa gacggatgtg 1500 tccaggaatc aactttgcga tccagatcat tcacatgaca ctggcacggc tacttcattc 1560 attcgagttg cgggtgccag aagaagaagt aatcgacatg gctgaagatt caggtctaac 1620 catatccaaa gtaacaccac ttgaactcct acttacccca cgcctccctc ttccacttta 1680 tatatga 1687 <210> 208 <211> 1569 <212> DNA <213> Tinospora cordifolia <400> 208 atggatttac cacaaccgac catatcaacc acacttctag cttttttcag catcttatta 60 ttcatcatat gctatcgtct cttgagcact aaatctgaca agagatcatc atcttccaga 120 aacaaacctc ttgacgctcc tggagcatgg cccgtcatcg gtcacctaca ccttctaaac 180 ggcgtcatac aacacgaaat tctgggaacc atggcggaca aatacggacc ggcgttcacc 240 atctggctcg gcacgcgtcc ggcactagtg gtaagcaact gggaagtggc taaggagtgc 300 ttcaccacct gcgataaggc cttagcaagc cgtcccccaa gcttagcttt gaagatcatg 360 ggctacaacg acgcgttatt cgcgttcgct ccttacgggc aatactggcg tgagttgcgc 420 aaaatagtga tgattgagct tctttctagt cggcagcttg attcgctgaa gcatgtttgg 480 gattctgaaa ttgattcatg catcgaagat ttgtatgaaa aatgtaaggg tcatcgtgaa 540 acagtactgg tgaatatgaa gcagtggttt gcggatttga tgatgaacgt agtggtgaga 600 atggtggttg ggaagctgaa ttttgggaaa acgaaagaag gtgatgatga ggtcgcgaag 660 gcgcaccaag ggcagttgaa ggcgatgaga gagttcttca agtgggtgga tgtgtttatg 720 atagaagacg cgtttccgat tctgacttgg ttggatcctc aagggtatca aagaaaaatg 780 aagaatatag cgaaagatct ggactcgctg gttcaggggt ggttagatga gcataagttg 840 aagcaaaaaa ctggcgataa tgggaagaaa gacttcatgg atatcatgct gtcggcggtc 900 aaagatgcta gtatatctga cagagacgat gagacaatct gcaaggcgac gtgcttgaat 960 ataattcttg gggcgagtga taccacaacg gtgaccctca catggaccct ttctttgtta 1020 ctaaataatc gacatgtctt gaggaaggcc caagaagaat tacaagcaca agttggcaaa 1080 cacagatgcg tggaagaatc agacgtcaag aaccttgttt atctacatgc catcatcaaa 1140 gaagtgctaa gactttaccc agctgttccg cttttaggcc cacgagagag tgtagccgat 1200 actgtggtgg caggctacca tatacccgca ggcacacggc taatcgtgaa cctttggaag 1260 attcatcgag acccgcttaa ctggcctgac ccgcttgagt ttcgacccga gagattcctt 1320 acgacccaca aagacacaga tgtgtggggt cagaattttg agttgactcc ttttggatcc 1380 ggaagaagga tatgcccagg tatttcgttt gcgcttcaag taattcctct cacactggct 1440 cgattgctcc atgggtttga gctcaccaca ccaggtagtg caccggtgga catgactgaa 1500 agcactgggc tcaccaactt gaaagccaca cctttagatg ttttcatcag tccacggctt 1560 gttctttga 1569 <210> 209 <211> 1581 <212> DNA <213> Tinospora cordifolia <400> 209 atggagtcca ttcccacagc aattaatgtg cttcttgcct tcgtctccat ctactatctt 60 ctgaagttcc ggaaaaagat catcacaacg actagagata tcaagaagat caaagcacca 120 gaactaaagg gtgcatggcc gatcataggt caccttcatc tattcagatc cgatgatctt 180 ctgcatcgaa agctcggtgc catggccgac gagttggggc cagcgtttat gatccggcta 240 ggtatgcgcc ctgctcttgt agtgagtaac tggcaaacag ccaaggagtg ctttactacc 300 aaagacagga tcttcgcaac ccgtcccgat tcggttgctg cgaagcacat gggctacgaa 360 aatttaatat tgggcttcac tcgttacgga ccttactggc gtgaggttcg caagattgtc 420 acgctcgagc ttctctccag ccatcggcta cacctgctca ggcatgtcag gatatcagag 480 attgacatgt ccattaagaa actccatcag ctttgtacca gtaacgacaa caaatattca 540 tcagttgtgg tcgacctgaa tcaatggttt aaagctttaa ctcttaatat aatcgtgaga 600 ataatagcgg gcaagcggta ttacggtgga gacctcgagg acgatgagga gtcgcggcgg 660 tggaagaaag ctcttaacga atttatgcat ctctttggaa tattttcggt gtcggatgcg 720 attcctcagt tgcagggtat ggacgtgcag ggacatgagc gtgtcatgaa gaggactggt 780 aaggatattg atgtgattct gagcaaatgg ttggaagagc acagaaggaa gaagatcagg 840 gttaacaggg aaggagctga gaaagatttc atagatataa tgcttgacct aatcaaagaa 900 aacgtcaata tctccggtca tgatgctgat aacgtcgtta aagccacttg tttggccctt 960 gtatcagcag gaagtgatac tacaatgatc actctaacat gggctgtgtc gttacttctg 1020 aataatcatc gagtcttgga gaaagctcaa gccgagttgg ataatcaaat tggaataaac 1080 agagtacatg tagaagaaga ggacatcaag aatctaccat accttcaagc aattgtgaag 1140 gagactcttc gcttatatcc tgctgctcca ttttcattgc cacatgaggc catggaggaa 1200 tgtactgttg caggcttcca tgttccgaaa gggacacgct taatcacaaa tatctggaag 1260 ctgcaacgag atccccaagt ctgggaggat cctttggagt tcaggccaga gagattcctc 1320 acaagtgatc gtcgtcatgt ggattttaga gggcaacatt ttgaattaat accatttgga 1380 tcaggaagaa ggatgtgccc tggcatttca tttgccattg gtgtcttgca cctgacgctt 1440 gcgcgtttac ttcatgaatt ccaccttgga actccgacca acgcacccat agacatgaat 1500 gaaaatccag gaattaccct cgagaaagca gatcctcttc atccactcgt ctctccaaga 1560 cttacttcca tcaactactg a 1581 <210> 210 <211> 1617 <212> DNA <213> Tinospora cordifolia <400> 210 atggattccc agttaacttc gtttcaatcc aagcccagtg catatctaat tgctgcactt 60 cttgccttag tttctgcata ctatctcatc atcaagaaga aaagcagagc aacttaccaa 120 gataactacg gcaagattag ggcaccggaa ccaaagggtg cgtggccaat catcggtcac 180 cttctcctgt tcaaacccaa cgatgtgctg tatcaaaagc ttagctcgct cgcagacgag 240 ttaggaccag cgttcatgat acggctgggc atgcgctcag ctctcgtaat aagtaactgg 300 gaaatagcca aagaatgctt caccaccaac gacaggatct tcgcgacccg tccaaagttg 360 atggcctcaa agcacatggg ctacggtggc gcagaagtcg gcgtcgcgcc ctacggtccc 420 tactggcgtg aggttcgcaa aatcatcacg gtagagcttg tctccaacca ccggctcaac 480 ttgctcaagg acgtcaggat atcggagatc gatatgtcct taaaagagct ctatgatctt 540 tgtatgaaga agaagaagaa cggcagtgga gaagaatcat cgcgggttga attggacctg 600 taccaatggt tcaaggacat gtcgctcaac atattggtca agctcatcgc gggcaagcgg 660 tattacgggg tggccgctgc cgccaacgaa gatgaggaat cgcggcggtg gaagaaagca 720 acggaggaat cggttttcct gtttggacaa tttgtggtgg gggatgcgat tcctttgttg 780 gagggtgtgg atgtgcaagg gcttgagcgt gccatgaaga agactggtaa ggaaattgat 840 gcagttttga gtaaatgggt ggaagagcat agaatgaaga agcagcagct taatggggca 900 agagatgagc aagatttcat agatgtgatg cttaacataa taaaggatgg caagatctct 960 gatcatgatg ctgatactat cgttaaagcc acttgtatgt ctcttgtaca agccggaagt 1020 gacaccacaa tgcttaccct gacttgggct gtgtgcttgc tactaaacaa ttgcaatgtt 1080 ttagagaaag ctcaagcaga gttggatgat caaattggaa gaagcaaagg acgtattgta 1140 gaggaagatg acatcaagaa actgccttat ctccaagcaa ttgtgaagga gacttggcgc 1200 ttgtaccctc ctgctccact tgctgtacca cgtgaagcca tggaggactg tactgtagct 1260 ggcttccatg ttccaaaggg tacacgctta atgacaaaca tatggaaact gcatcgagat 1320 cccaaagtgt gggacgattc tttaaagttt aggccagaaa gattcctctc cagtgaacat 1380 gcacatgtgg attttagagg gcaacagcat gagtttatgc cctttggatc cgggagaagg 1440 atgtgccttg gaatgccatt agctattggt gtggtacacc ttacacttgc ccgtttactt 1500 catgaattcg agcttgcaac tccgtcaaat gcaccagtag atatgagtga aaaagctggt 1560 cttactttgg ttaaagcgac gcctctaaat gttcttatcg ccccacgcct ttgctaa 1617 <210> 211 <211> 1596 <212> DNA <213> Tinospora cordifolia <400> 211 atggattcaa taatactcca gagtaacctt cttaccactg ttataataat aacatccttt 60 ggcctctttg tcctttcctt ttactatgtt ctctggaagg caattagaac tggcaacaag 120 agaaattgca gcaaccctaa ggctcctgaa gctgctggag gatggcccat tctcggtcat 180 cttcatttat tcagaggcca aggcctatta ctgcacaaaa tttttggagc catggccgaa 240 aaacacggac cagcattcac cattcggctc ggaatgcgtc cggcactagt tgtgagcaac 300 tgggaagtgg ctaaggagtg tttcaccgca aatgatcggg cccttgcgag ccgccctacg 360 agcttagcct tgaagattat gggctacaac aacagcttgt tcgcgttcgc tccatacggg 420 caatattggc gtgagttgcg caagatagtg atgcatcagc ttctctctag ccgtaggctc 480 gagttactca agaacgtttg gctttctgaa attgatatat ggatcaaagg cttgtacgag 540 aattccttgg ttaataaagt ggtggatatg aagcagtggt ttggggaatt gatgatgaac 600 atagtggtga gattggtagc aggaaagcga agttttggga aaatcagaga aggcgatagt 660 gaggaagcga aggcgcatca gcggcaaatt aaggcactgc gagatttctt caggttggtg 720 gaggttttta tgatagaaga tgcgtttcct tttctaactt ggttggatcc tcgagggtat 780 caaaaagaaa tgaagaatat agcaaaagaa ctggattatc ttcttgagga gtggttagaa 840 gagcataaac tgaagcaaca agctgcatgt gatgatcaga gcaaggactt catggatgtt 900 atgctgtcgg aggtcaagga ttctactatc actgaaagag atgcaaacac aatctgcaaa 960 gcgacttgcc tgaatgtaat tttaggtgcg agtgatacca caactctgac tctcacatgg 1020 gccctttccc tactactgaa caacagacac gtattgaaga aggcccagga agaattaaat 1080 gcccaagttg gcaaggataa acaagtggaa gaatcagaca tgaagaacct tgtgtaccta 1140 catgccataa tcaaagaagt tctaagactc tacccagctg ctcctttgtc agtggcacat 1200 gagtctttag aagacaccgt ggtggctggt taccatgtct ccaagggcac acaggtcata 1260 ttcaaccttt ggaaaattca acgtgatcca tctgtatggt ccaacccgct agaattccaa 1320 cctgagagat ttctcaccac ccacaaacat gtagacattt ggggtcagaa ttttgaattg 1380 ataccgttcg ggtcgggaag acggatgtgc ccaggcgttc ctttcgctct tcaagtggtt 1440 tccttaacgt tggctcgatt acttcatggg ttcgagctca caacaccagg tggcgcagct 1500 gttgacatga ccgagactcc tgggctcact aatatgaaag caaccccggt agaggttttc 1560 cttagacctg acctccctca ccagctctat gcttga 1596 <210> 212 <211> 1575 <212> DNA <213> Thalictrum flavum <400> 212 atgccaagcc aaaccataca aattataatg gatttctttc atcaatgtgt tgcaactata 60 ttattttgtg tcttagcttc accttttttc ttttattatc tactcccatg gtggaaaaat 120 gctaatgatc atgatcttgc caagagcaga ggaaagcctg ttcctgaagt agatggtgca 180 tggcctatta tcggtcatat gcatatgctg caaccatcag atagctttta ctcatctttg 240 gcggaaaagt atggaccaat tttcacactt cgaattggtt tatgcaaaac aattgtgatt 300 aatagttggg agctagctaa ggaatgttgt accactcatg atagagtgtt tgcaagctcg 360 cctgaagcta cagcggcaaa gatattggga tatgattatg ctatgtttgg acgaaaccct 420 tatgggcctt actggcgtga aatgcgcaaa ataattataa ctgaacttgt gtcaaatcgg 480 agactggagc tactaaaaca tattagagta acggagatca gtacatccat acaagaattg 540 taccagttgt gggaagcaag gagtagtaag aatgtaaaag aaagagttgt ggtggatatg 600 caaaaatggt ttggtgactt gatgctaaat attggtgtcg agatggttgc tggaaagaga 660 tattttggtg caagttcgaa tgaagataaa gaggaagcaa gacagttgca taagacattt 720 aaagatttct cgaacctatt tggagtgcct gtgctatctg atgcaattcc atttcttagg 780 tggttagact ataaaggaca tattaaagcc atgaagagat gtgcaaaaca agtagattgt 840 atcttgcata gatggttgga ggaacataaa caaaacaaga caacaattgc tggggatttc 900 atggatattt tgttgtcagt acttcaggat aaagagatct ttggtaggga tgcagacacc 960 gtaatcaagg ctacatgtct gaatatgatg ctgggtgttg tagaaaccaa taaggtcact 1020 gtaacaatgg cactcatctc attgttgaac aatcgtcgca ttttgaagaa ggcacaagaa 1080 gaaatagaca ttcatgttgg gagcgataaa caagttgaag aatcagatat agagaaactt 1140 gtatatctcc aagccattgt caaggaaacg ttgcgcttgt actcacctgg tctaggaaca 1200 cgtgagccct cagaggactg caccatatca ggtttccatg ttccaaaagg cacagaggta 1260 atggtaaatg ttcagcagat tcatcgagat ccaagcattt ggtctaaccc tcttgaattt 1320 caaccagaga gattcctcac cacacaagca aatatagact ttgggggtca acatcatgaa 1380 tacattcctt tcggggcagg ccgtagatta tgccctgcaa tctcattcgc agttctggtg 1440 gtgcacttga cactagcacg tttgctgcaa gggtttgatt ttgcaacacc aaaggatgca 1500 cctgtggaaa tgaatgagag tgtggaaact ctggaagttt taatcacccc acgactccct 1560 ttgcatatgt attaa 1575 <210> 213 <211> 1605 <212> DNA <213> Xanthoriza simplicissima <400> 213 atgcacaatc ccataccaaa ccaatggcta ccaagttcaa ctcttctgac gcctatcatt 60 gttagtttcc tcgttatcgt tctcttctat atctataaga agagaagtag taacacgatc 120 aggaccaaga aggcgcctga agtagtagga gcatggcctg taattggaca tctaaattta 180 tttagttgtc ctaaaccggg tcatatagtg ctgggcgaac tagctgatca atatggcccc 240 gcatttacta tccattttgg tatgcatcca acactggtgg ttagtagttc ggagctggtg 300 agggactgtt tcactacgaa tgacaaatta ttttccagcc gtctagttaa caaagccatc 360 aagtacatgt tctacgatca ggacactatt tcattcgcac cttatgggcc ttattggcgt 420 gagcttcgta agatgattgc tcttaatctt ttaaataatg aacgcatcaa aatgctccag 480 cagcaaagga tttcagagat ggatgcttgt ttaaaaaagc tgtacgattt atcagcaaaa 540 agaaaagacg agaacgcagg ggtattggtg gatatgagta aatggtttgc cgaaatttcc 600 tttaatgtgg ttaccagaat tgttgctgga aaacatattt ttgggcctaa agttgaaaga 660 tacaagaatg tgatggaaga ggcacgccgt cttatggacg tcatggtttt ctccgacatg 720 gtaccttatt taggatggtt ggatagattg cgaggagtgg acagtgctat aaagcggaca 780 gccaaagaat tggactctgt tcttgagagc tgggtggagg aacatcgtcg aaagagcgtg 840 tcaatctcag ctggaacggg aggcattttc aacataaaag aagaggagga attggatttt 900 atagacatta tgttgtcgat tatagccaag aacaatttac caggtgacga tcctgatact 960 ttaatcaagg ctattgtcca ggaaacgtac cttgctgcgt gggacaacac aacggtaact 1020 cttacatggg tcttgtgttt gttattaaac aataagcaag ttctgaaaag agctcaaaat 1080 gaattagacg ctcaagttgg gaaggagagg caagtagaag attcagacat taataatctt 1140 ccctatgtac aagccattgt taaagaatca ttgagattat atccaccagg accgataatt 1200 gaacgggcga ctactgagga ttgtaacgtt ggtggcttcc gcgtcccggc aggtacacgg 1260 ctttgggtaa acttatggaa gttacaacga gacccaaaag tatggcctaa tgatccgcta 1320 gaatttcgac cagaaagatt tctcaatgac aatgcagaca ttgatttgag gggtcagaat 1380 tctgaattaa taccatttgg atcagggagg cgcatgtgtc ccggtgtctc attttctctt 1440 caagtcatac atctggtgat tgctcgaata attcagggat ttgagttaaa ggccccaaca 1500 gatgcagata tcgacatgtc tacaacgcta ggaatgataa gctggaaagc aacaccactt 1560 gaagtactca tgaatccacg cttcccaccc gtgttttaca agtga 1605 <210> 214 <211> 1653 <212> DNA <213> Xanthoriza simplicissima <400> 214 atgtctacat tacaaattag cattttaaca gttaaatccc cgtattcaca aatgcattat 60 tatccccttc ttcaccaatg gctaccagct tcaatggcgc tagtcagttt gctcgttatc 120 attctcttct ctatcttcaa gaagagaagt agtaagatga tcaaggccaa gaaggcgcct 180 gaagtagcag gtgcatggcc tctaatcgga catctcaatc tatttagtgg tcctaagcta 240 cttcatttag tacttggaga actcaccgat caatatggac cggcctttat gatccatctt 300 ggtatgtatc caactttggt ggtgagtaat tgggagcttc taaaggactg tttcactaca 360 aatgacatat tcttctcaaa tcgtccagtt aacaaagcga tcaagcacat gttctacaat 420 aaagagtcta ttggattcac accttatgga tcctattggc gtgagcttcg taagatgact 480 actctcaaac ttctatcaaa ccaccgcctt gatctgctca agcccttgag aatttcagag 540 atggacgctt gttttagaaa cctttatgag ttgtggacga aagataaaga cgggaatgca 600 tggctgttgg tggatatgag taaatggttt ggcgagatct cgtttaatgt ggttgcaaga 660 attgtagctg gaaaaaagaa ttttgggtcc aaaggtgata ggtataagac agttatggaa 720 gaggtggtcc gtcttatggg tctcagagct ttgtcggacg cggtaccata tttaggatgg 780 ttggatcagt tgcgaggact ggacagtgct atgaagcggg cggccaagga attggattct 840 gtgcttgaga gctgggtgga ggaacaccgc ctaaagaggg tgtcagtttc agctggaact 900 ggaagcactg taaagacagc aaaagaggag gaggaggagg aggacttcat agacattaca 960 ttgtcgattt tggcggagaa ccaattacca ggtgatgatc ctgataccgg catcaagtct 1020 cttatcctgg acatgatcct gggtgggagc gacacttcaa cggtgacact aatatgggca 1080 atgtgtctgc tattaaacaa tgtgcatgtg ttgaaaaggg ctcaatatga attggacgca 1140 cagattggga aggaaagaca agtagaggat tcagacataa agaatctgcc ctacatccaa 1200 gccattatta aagaaacaat gagattatac ccagcaggac cgattatcga acgccaggct 1260 aatgaggact gcgacgtggg tggcttccat gtcccagcag gtacacggct atgggtaaac 1320 ttatggaagt tacaacgaga cccaaatgta tggaaggacg acgcattaga gtttcgacct 1380 gaaagatttc tcactgatca tgcagacatt gatttgaagg gtcagcatct ggaattaatt 1440 ccatttggat caggtaggcg tatatgtcct ggtatctcat ttgctcttca agtcatgcat 1500 ttggcacttg ctcgaattat tcatggattt gagttgaaga ccccaaatga ttcaaatatc 1560 gacatgtctg gaactcctgg acttttatgc tgcaaagcaa caccactcca agtactcctg 1620 actccacgtt ttaaccccat gttttataag tga 1653 <210> 215 <211> 1563 <212> DNA <213> Xanthoriza simplicissima <400> 215 atggattcac ttcgacattg tttaggaggt attcttgcct tactaatctt tcttggttat 60 ctgcaatgga agagaggaac aagtaacaag tgcatagaag ctcctcaacc ggatggggca 120 tggcctatca taggccattt gcctaggttg atgaaacccc aaataatgca tagaacgttg 180 agcaccatgg ctgataaaca cggcccagcg tttactctcc ggcttggcgt acataaaaca 240 ttggtggtga gtagttggga ggtagccaag gagtgtttca ctaccaacga cagagtcttc 300 gctacccgcc caacctctat cgccgtggag atattgggct acaactacgc cttatttgcc 360 tttggtcctt atggctcata ttggcgtgaa gcccgcaaga tagcaatact tgagcttctc 420 tcaaaccata ggctcgagtt gctgaaacat gttcggatct ctgaggtgag cacatctata 480 agagaattgt accaagtgtg gaaagaaaat tgtgatatag caaatggatc tgctttggtg 540 gatatgaaac catggtttgg tgacttgaca ctaaacgtgg ttgttagaat ggttgctgga 600 aaacggtaca tgggtggcag tgttaaatcg gatgatgttg agggaaggcg atttcagaag 660 gccactaaag atctctttga tttatttgga ctgtttatat tgtcagacgc gcttccattt 720 atgggttggt tagacctcca tggccataag aaagccatga aaaaaactgc caaggagctg 780 gactccatta tgcagagatg gttggaagaa catcgacaaa gtttattgga tgatggaaca 840 aaggaggaga agaaggactt catgtatgta atgttatcta tccttgagga taaaaagcta 900 tttcaatatg atgccgacat cgttaacaaa gctatttgca tgaatatgat tatggcaggt 960 actgatactc aaatgatcac tctaacatgg gtcctttctt tactactcaa caatcgacac 1020 attttaaaga aggcccaaga tgaaatcgac tccagtgttg gaaaagatag acaagtcgag 1080 gagtcagaca tagtgaaact agtgtacctc caagcaattg tgaaggaagc attgcgtctc 1140 tacccacccg ctcctctatc cgcacaacat gaggccatgg aggactgcac tgtggctggc 1200 tactatgtcc cagctggcac acgcctaata acgaacatac ggaagattca acgagatcca 1260 cgcgtatggt ctgacccttt tgagttccat ccagagagat ttctcacaaa ccaagcaaat 1320 gtggacttta gaggtcaatc ttttgagttc attccttttg gatctggaag acgaatgtgc 1380 ccaggcattt catttgcgct tcaagtggtg catttgacat tagcacgtat cctacaaggt 1440 tttgattttg aaacaccaat gaatgcttct gtggacatga ctgaggcacc aggtctgaca 1500 aatgttaaat caacccctct tcaagttcta atcacccccc gtctccaccc aaacctatat 1560 taa 1563 <210> 216 <211> 1572 <212> DNA <213> Xanthoriza simplicissima <400> 216 atggatttac ttaacccata ttttgcaact atctttggtg gtttctttgc gttactaatt 60 tttcttttta tcatttcaca gaagagaagt aggattccca agatcagagc agctccagaa 120 ccagttgggg catggcctat cgtaggacac ttgcctatgt tacttggacc cagattaccc 180 cattttgtgt tgggagacct tggagagaag tacggatcag catttactct ccggcttggc 240 atacataaaa cattagtggt gagtagttgg gaggttgcca aggagtgttt tactacaaat 300 gaccaagtat tcgccacccg ccctagcttc atggcagcga agataatggg ctataattat 360 gctctatttg gcttagcacc ttacgggtca tattggcgtg aactccgtaa aatatctatt 420 attgagcttc tttcaagtca taggcttgaa ttgctgaagc atgttcgagt ctctgaggtt 480 agtacatcaa tgaaagaatt gtacgaggtg tgggcagaaa actgtagtaa tggaaacgga 540 tctgttttgg tggagatgca gcgatggttt ggtgacttga cactaaatat aagtgttagg 600 atggttgctg gtaagcgata ctttggtact agtgctagtt tggatgacga cgaggcaagg 660 cgaatatcaa aggccacaaa agatttcttc cgtttgacag ggatgtttgt ggtgtcggac 720 tcggttcctt tcctttggtg gctagatttt cagggccacg agaaagccat gaagagaacc 780 gccaaggaaa tggacgatat atttggggga tggttggagg atcatcgacg aaataaactt 840 gctggtggaa caaaggtgca gcaagacttc atggatgtga tgttgtctat ccttgaggac 900 gaaaagttct ttggttataa tgccgatgtt gtcacaaagg ctacttgcct gaatatgttg 960 ttgggtggga cggataccac aatggtcacc ttgacatggg ccctctcatt actactgaac 1020 aaccgtcata ttttgaagaa ggcccaaact gaaattaaca ctcatgttgg aaaagataca 1080 caagttgatg aatcagatat agtgaaactt gtgtacctcc aagcaattgt caaggaaaca 1140 ttgcgtctct acccagctgc tccactatcc acaccacacg aggccactaa ggactgcacc 1200 atcgctggct accacgtcac tgcagggacg cgcttgataa caaatatatg gaagattcaa 1260 cgagacccac gcgtatggtc caaccctagt gagtttcaac cagaaaggtt tctcactgat 1320 caagcaaatg tggacgttag gggccaacat tttgaattca ttccttttgg gtccggtaga 1380 cgatcctgcc caggaatctc gcttgggcta cttgtggtgc agttggcatt agcacgtttg 1440 ctacaaggct ttgatttcga aacaccatcg gatgcattgg tggacatgac tgagagcgct 1500 ggattaacta atctcaaagc aacacctctt catgtcctaa ttacaccgcg tctccattca 1560 agcctgtatt aa 1572 <210> 217 <211> 1593 <212> DNA <213> Xanthoriza simplicissima <400> 217 atggactcag ttcaccaagg ccaataccta ccaactccga tggtcagtgt ctttgtgttt 60 tttatcagtc tttactttct aattgtgtgg aagacaaggt ctagttccaa gactaacact 120 tgcaacgaag tacccgaagc gcctggggcg tggcctatta tcgggcacct ccatttgtta 180 ggtgggagtg aattacccca caagacatta ggagccatgg ctgataagta cggcccaatc 240 tttaagattc gtattggtgt gcatcaagca ttggtggtga gtagttcgga tattgtaaag 300 gagtgtttca ctactaatga taaggttttt gcttctcgtc ctacctcgac agcatcaaag 360 atcctgggct atgattatgt catgtttggt ttctctcctt acggaccgta ttggattgaa 420 ctacgcaaaa taattatgtc ggaacttctc tcaaaccgca ggctggaact actaaaacat 480 gttagggact ctgaaatcga tatatctatc caagaactat acaaggtgtg gaagaatcat 540 gataagtcaa aaggtccaat cttagtagac atgaagcaat ggtttggaga cttggcactc 600 aatgtgattc taaggatgat tgccggaaag agatactctg gttcaatctt ttcttctgat 660 gagacagaag caaggagatg tcaaaaggga gcgagagatt tctttaggct gttggggcta 720 ttcatcattg aagatgcact tccgtatcta agttggttcg acttacaagg ttacaagaaa 780 gagatgaaga acacggcaaa agaacttgat tctgtttttc agagatggct ggaagagcat 840 aaacgaacga gagaaaccgg tgaactgaat cgggagcagg acttcatgga tatcttaatg 900 tccacactgg aagagacaaa gatatctgaa tacgacaatg acactatcat caagtctact 960 tgcctgagca ttgtaacagg tggaaatgac actacaatgg ttactctcac ttggatccta 1020 tccttacttc taaacaataa acatgcactg aagaaggttc aagatgagct ggacagccac 1080 gttggaaagc atagacacgt ggaagagtca gatatcaaga acctcatcta cctccaagct 1140 ataatgaagg aagccttgcg gttgtaccct gcaggtccac tatcaggtcc tcgggtggca 1200 gacgctgact gcaccttagc aggctaccac atcccagcag gcacccgctt acttgtgaat 1260 acctacaaga ttcaaagaga tccattggtg tggtctgagc catctgagtt ccgaccagag 1320 agattcctca ctagccatgt aaacatggat gttaagggtc tgcaatatga attaatacct 1380 tttgggacag gtaggcgtgc gtgtccaggg atgttatttg cgcttcaagt ggtgccacta 1440 gtcctggctc ggtttctaca tgagttcgag ccaaagactg aaatggatat gccagtggat 1500 atgacggaga ctgcgggact aagtaacgcc aaagcaacgc cactagaagt agtaatcacc 1560 ccacgccttc agcccgagct ttatagttta taa 1593 <210> 218 <211> 1587 <212> DNA <213> Xanthoriza simplicissima <400> 218 atggacttca ccatgatcat ccaatggctt ctcacatcct ttgctattct tgttgcattt 60 gttctaatct ggtctagtgc aacaagatct agtactagga aaagaaacaa aacagcacct 120 gtagctgctg gtgctttgcc cattgtagga cacctacata tgctaatggg acgagaactg 180 cctcatcgat tgctttcaaa tatggctgac aagtatggtc ctgccttcat gatgaagtat 240 ggcttacaac ctgcacttgt ggtgagcagt ttgaagctca ctaaagagtg ttttgttcac 300 aacgatagag ctgtttttaa acgtccaaga agcaaagcat tgaaaaactt gacctatgat 360 caagcttctt tgggttttgc gccatatgga ccactttggg tggagatgcg aaaagtatct 420 aaaactaatc tcctctctaa ccaaaggctt caaatgcaaa gacatgtaag agcctcagaa 480 gtcgacgctt tcatcaaaga gctttacgat ctatggtctt ccaaatccaa caacatcaaa 540 ggcgcgccac ttttggttga aatgaataaa tggtttgaag aactggcact taacgtagtg 600 accaggatgc tgagtggaaa aagacacatt ggctttaagg caaggcgtgg tgaagatagt 660 gaatctatgc attataagaa agttgtagtt gatgctactc ttttaactgc aaagcttgtt 720 gtgtcagatt tctttccttc tcttggattg gtggactact tgcaaggtga cgagagctct 780 ataaagggga caagcaaaga actagacgcg atcctcgcca cttgggtgga agagcatcgc 840 cataagaagg tttcaggctc agaggatgat gagaaagact tcattgatct cactttgtcc 900 atgatagatc aaactcagca ccaaggtgca gatgtcgaca ccttcatcaa gtctatgtgt 960 gtgggcatga tctttggtgg gagtgatagc acatcagtgg ccttagcatg ggctctttcc 1020 atactgatga ataatcgtcc tgttttgaag aaagcccgag aagaaatcga tcgacaagta 1080 ggtagagata ggaaagtgaa cgatttggat gtcctcaaat tggattatct caaggcaatc 1140 gtaaaggaat cgatgcgatt atgcctagtc ggaccgcttc ttgaacgcgt cgccgtagaa 1200 gattgtgaga taggcgggta tcatgttaaa gcaggctcgc gcgtagtagt gaatatttgg 1260 aaaatgcaac atgatccaaa cttatggtct gatcctttgg aattccaacc agagagattt 1320 ctcactacaa acgcaaatgt tgaacttaga ggccagcatt tcgaactcct tccatttgga 1380 gctggtagtc gtatttgtcc tggaattaca tttgccctcg aacttattca gttaacactt 1440 gctcgtctaa ttcatggatt tgaactggga actccgatgg atgcagacgt cgacatgaca 1500 gaaacatcaa gtgttaccaa ctatagggca acacctcttg aaatattact aacaccacgc 1560 ctggatccaa agctttacaa ttattag 1587 <210> 219 <211> 532 <212> PRT <213> Argemone mexicana <400> 219 Met Asp Asn Phe Leu Leu Gln Phe Gln Pro Ile Ser Ile Ala Ala Phe 1 5 10 15 Phe Val Ala Leu Val Phe Leu Tyr Trp Ser Tyr Gly Arg Lys Ser Asn 20 25 30 Lys Thr Leu Lys Pro Arg Ala Pro Glu Ala Ala Gly Gly Arg Pro Ile 35 40 45 Thr Gly His Leu His Leu Phe Tyr Gly Glu Glu Leu Thr His Arg Lys 50 55 60 Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Val Phe Asn Ile Arg Phe 65 70 75 80 Gly Ser His Lys Thr Leu Val Val Ser Asn Trp Glu Ile Val Lys Glu 85 90 95 Cys Phe Thr Thr Asn Asp Arg Leu Phe Ser Asn Arg Pro Gly Thr Leu 100 105 110 Ala Ile Lys Leu Met Phe Tyr Asp Thr Asp Ser Val Gly Tyr Ala Pro 115 120 125 Tyr Gly Asn Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr Leu Lys Leu 130 135 140 Leu Ser Asn His Arg Ile Glu Thr Leu Lys His Leu Arg Thr Ser Glu 145 150 155 160 Val Glu Ser Cys Phe Lys Gln Leu Tyr Asn Gln Trp Glu Met Lys Asn 165 170 175 Glu Asn Arg Val Glu Val Asp Lys Leu Gly Phe Ala Ser Val Arg Met 180 185 190 Asp Asn Trp Phe Gly Asp Leu Thr Phe Asn Val Val Ala Arg Ile Val 195 200 205 Ala Gly Lys Lys Asn Phe Ala Gly Gly Ala Val Asn Gly Asp Ile Gly 210 215 220 Ala Gln Arg Tyr Lys Val Ala Met Asp Glu Ser Phe Arg Leu Met Leu 225 230 235 240 Thr Phe Ala Tyr Ser Asp Val Ile Pro Ser Leu Lys Trp Leu Asp Lys 245 250 255 Leu Arg Gly Leu Ile Arg Asp Met Lys Arg Cys Gly Ser Glu Ile Asp 260 265 270 Ser Ile Val Ala Ser Trp Val Glu Glu His Arg Leu Lys Arg Asn Ser 275 280 285 Ser Glu Asn Asn Asn Leu Asp Leu Glu Glu Asp Phe Ile Asp Val Cys 290 295 300 Leu Asp Ile Ile Glu Ser Ser Ser Leu Pro Gly Asp Asp Pro Asp Thr 305 310 315 320 Val Ile Lys Ser Thr Cys Leu Asp Met Ile Leu Gly Gly Ser Asp Thr 325 330 335 Thr Thr Val Thr Leu Thr Trp Ala Ile Ser Leu Ile Leu Asn Asn Pro 340 345 350 His Val Leu Lys Arg Ala Lys Glu Glu Leu Asn Ser Val Val Gly Lys 355 360 365 Asp Arg Arg Val Glu Asp Ser Asp Ile Pro His Leu Thr Tyr Ile His 370 375 380 Ala Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ile 385 390 395 400 Glu Arg Arg Thr Met Glu Asp Cys Glu Val Gly Gly Tyr His Val Pro 405 410 415 Ala Gly Thr Arg Leu Leu Val Asn Val Trp Lys Met Gln Arg Asp Gly 420 425 430 Asp Val Tyr Lys Asp Asp Pro Leu Val Phe Arg Pro Glu Arg Phe Met 435 440 445 Thr Ser Asn Ala Asp Val Asp Leu Lys Gly Gln His Phe Glu Leu Ile 450 455 460 Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser Phe Ala Val 465 470 475 480 Gln Leu Met His Leu Val Leu Ala Arg Leu Leu His Glu Phe Glu Ile 485 490 495 Thr Thr Ile Pro Ser Glu Pro Lys Val Asp Met Ser Glu Ser Gly Gly 500 505 510 Leu Ile Cys His Lys Ile Lys Pro Leu Glu Val Leu Ile Lys Pro Arg 515 520 525 Leu Glu Leu Asn 530 <210> 220 <211> 537 <212> PRT <213> Argemone mexicana <400> 220 Met Asp Phe Ser Ser Ile Leu Leu Leu Leu Asn Ile Asn Cys Phe Thr 1 5 10 15 Thr Ser Leu Val Thr Leu Leu Val Leu Val Met Val Phe Leu Tyr Asn 20 25 30 Asn Thr Arg Phe Thr Lys Pro Lys Gly Asn Lys Lys Met Ile Lys Pro 35 40 45 Pro Arg Leu Pro Gly Ala Arg Pro Leu Leu Gly His Leu His Leu Phe 50 55 60 Gly Pro Gly Glu Leu Pro His Lys Val Leu Ser Thr Met Ala Asp Lys 65 70 75 80 Tyr Gly Pro Ala Phe Thr Ile Lys Phe Gly Lys His Thr Thr Leu Val 85 90 95 Val Ser Asp Ile His Thr Ile Lys Glu Cys Leu Thr Thr Asn Asp Ile 100 105 110 Leu Phe Ser Asn Arg Pro Ser Ser Ile Ala Phe Asp Leu Met Thr Tyr 115 120 125 Ala Asp Asp Ser Val Ala Phe Ala His Tyr Ser Pro Tyr Trp Arg Glu 130 135 140 Leu Arg Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn Asn Arg Leu Gln 145 150 155 160 Ser Ile Lys Gln Leu Arg Leu Ser Glu Val Asn Val Cys Phe Lys Glu 165 170 175 Leu Tyr Ser Leu Cys Asn Asn Asn Asn Lys Ser Gly Ala Pro Val Leu 180 185 190 Ile Asp Met Lys Lys Trp Phe Lys Glu Val Thr Thr Asn Ile Val Ile 195 200 205 Arg Val Ile Val Gly Lys Gln Asn Phe Gly Ser Lys Ile Val Arg Gly 210 215 220 Glu Asp Gln Glu Ala Ala Asn Tyr Lys Lys Ile Met Asp Glu Leu Ser 225 230 235 240 Arg Leu Ala Lys Leu Ser Met Phe Ser Asp Tyr Ala Pro Leu Leu Ser 245 250 255 Leu Phe Asp Tyr Phe Arg Gly Asn Val Ser Ala Met Lys Arg Asn Gly 260 265 270 Lys Glu Leu Asn Ala Met Leu Gln Asn Trp Leu Glu Glu His Lys Arg 275 280 285 Lys Lys Asn Ser Ser Ile Ser Asn Asp Asp Glu Gln Asp Phe Met Asp 290 295 300 Leu Met Leu Ser Ile Ile Asp Glu Thr Lys Leu Tyr Gly Arg Asp Ala 305 310 315 320 Asp Thr Phe Ile Lys Ala Ile Ser Leu Ser Met Ile Val Gly Gly Ile 325 330 335 Asp Thr Val Val Val Thr Leu Thr Trp Ile Leu Ala Leu Ile Met Lys 340 345 350 Asn Pro Phe Ala Leu Lys Lys Ala Gln Glu Glu Leu Asp Phe Phe Val 355 360 365 Gly Lys Glu Arg Gln Val Glu Asp Ser Asp Leu Lys Asn Leu Val Tyr 370 375 380 Met Asn Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Leu Ser Cys 385 390 395 400 Ile Leu Glu Arg Asp Thr Lys Glu Asp Cys Glu Val Gly Gly Tyr Tyr 405 410 415 Val Glu Gly Gly Thr Arg Leu Leu Ile Asn Ala Trp Lys Leu Gln Arg 420 425 430 Asp Pro Asn Val Trp Thr Asp Pro Ser Glu Phe Lys Pro Glu Arg Phe 435 440 445 Leu Asn Glu Asn Ala Asp Ile Asp Val Gly Gly Gln His Tyr Glu Leu 450 455 460 Ile Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala 465 470 475 480 Leu Gln Phe Met Asp Leu Val Leu Ala Arg Leu Ile His Gly Tyr Glu 485 490 495 Leu Gly Thr Leu Asn Gly Glu Asp Val Asp Leu Thr Glu Lys Thr Glu 500 505 510 Gly Gln Ile Asn Phe Lys Ala Thr Pro Leu Asp Leu Ile Val Thr Pro 515 520 525 Arg Leu His Pro Asn Leu Tyr Asp Tyr 530 535 <210> 221 <211> 522 <212> PRT <213> Berberis thunbergii <400> 221 Met Glu Ser Ile Glu Phe Leu Val Thr Leu Leu Leu Ser Leu Leu Ala 1 5 10 15 Leu Phe Cys Ala Tyr Ala Trp Lys Arg Lys Asn His Thr Lys Asp Ser 20 25 30 Lys Ile Lys Glu Pro Pro Gln Pro Ala Gly Ala Trp Pro Ile Ile Gly 35 40 45 His Leu His Leu Ile Ser Arg Gly Gly Leu Pro His Ile Asn Met Gly 50 55 60 Ala Met Ala Asp Lys Tyr Gly Pro Val Phe Met Ile Arg Leu Gly Val 65 70 75 80 Asn Arg Ala Val Ile Val Ser Asn Thr Glu Ile Val Lys Glu Cys Phe 85 90 95 Thr Thr Asn Asp Lys Phe Leu Leu Asn Arg Pro Val Gly Leu Ala Leu 100 105 110 Asn Leu Met Cys Tyr Asn Asn Ala Met Phe Gly Phe Cys Arg Tyr Gly 115 120 125 Pro Tyr Trp Arg Glu Ile His Lys Ile Val Met Leu Glu Leu Leu Ser 130 135 140 Asn Ser Arg Leu Glu Ser Leu Lys His Val Trp Asp Ser Glu Ile Ser 145 150 155 160 Thr Ser Ile Glu Glu Leu Tyr Gln Leu Cys Gln Thr Gln Asn Lys Thr 165 170 175 His Glu Gly Pro Val Leu Val Glu Met Lys Asp Trp Phe Ala Asp Met 180 185 190 Ala Leu Asn Val Ser Leu Arg Met Ile Ala Gly Lys Arg Tyr Phe Gly 195 200 205 Ala Ser Ala Gly Gly Asn Lys Asp Glu Ala Arg Lys Cys Gln Lys Thr 210 215 220 Ile Arg Asp Phe Phe Arg Leu Val Gly Leu Phe Ile Val Ser Asp Ala 225 230 235 240 Leu Pro Phe Leu Arg Trp Leu Asp Leu Gly Gly His Gln Lys Glu Met 245 250 255 Lys Arg Thr Phe Glu Asp Leu Asp Tyr Met Leu Gln Gly Trp Leu Asp 260 265 270 Glu His Lys Leu Asn Arg Lys Ser Gly Val Asn Gly Asp Gln Asp Phe 275 280 285 Met Asp Val Met Leu Ser Ile Leu Asp Asp Ser Lys Phe Pro Asp Tyr 290 295 300 Asp Val Asp Thr Val Asn Lys Ala Thr Cys Phe Gln Leu Ile Ile Gly 305 310 315 320 Gly Thr Asp Thr Thr Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Met 325 330 335 Leu Asn His Pro His Val Leu Lys Lys Ala Gln Asp Glu Leu Asp Ile 340 345 350 Gln Val Gly Lys His Arg Gln Val Glu Glu Ser Asp Ile Lys Asn Leu 355 360 365 Lys Tyr Leu Glu Ala Val Ile Lys Glu Thr Leu Arg Met Arg Pro Val 370 375 380 Gly Pro Leu Leu Gly Pro Arg Glu Thr Ile Glu Asp Cys Thr Ile Gly 385 390 395 400 Gly Tyr Arg Val Arg Ala Gly Thr Arg Val Met Val Asn Ala Trp Lys 405 410 415 Val His Arg Asp Pro Ser Val Trp Ser Asn Pro Asp Asp Phe Lys Pro 420 425 430 Glu Arg Phe Leu Lys Lys Asp Ile Asp Phe Arg Asp Gln Asn Phe Glu 435 440 445 Phe Ile Pro Phe Gly Ser Gly Arg Arg Ala Cys Pro Gly Ile Ser Phe 450 455 460 Ala Val Glu Val Leu Pro Met Ala Leu Ala Arg Leu Leu His Gly Phe 465 470 475 480 Glu Leu Lys Thr Gln Leu Gly Cys Lys Val Asp Met Thr Glu His Ala 485 490 495 Gly Leu Val His Ala Lys Ala Thr Pro Leu Glu Val Leu Val Ser Pro 500 505 510 Arg Leu Ser Arg Glu Leu Tyr Val Ser Ser 515 520 <210> 222 <211> 514 <212> PRT <213> Berberis thunbergii <400> 222 Gly Ser Leu Ala Leu Ile Ala Ile Ile Ile Leu Ser Asn Ile Val Lys 1 5 10 15 Lys Ser Ser Lys Ser Leu Ser Ser Lys Gly Gly Lys Ser Ala Pro Val 20 25 30 Val His Gly Ala Trp Pro Val Ile Gly His Leu His Leu Leu Met Gly 35 40 45 Lys Glu Leu Pro His His Ala Leu Ala Lys Leu Ala Asp Lys Tyr Gly 50 55 60 Pro Ala Phe Thr Leu Asn Ile Gly Ala Tyr Gln Glu Leu Val Ile Ser 65 70 75 80 Thr Arg Asp Leu Ala Lys Glu Cys Phe Thr Thr Lys Asp Lys Phe Phe 85 90 95 Leu Asn Arg Pro Ser Asn Lys Ala Met Lys Ile Leu Thr Tyr Gly Glu 100 105 110 Ala Ser Val Gly Phe Ala Pro Tyr Ser Pro Leu Trp Val Glu Met Arg 115 120 125 Lys Val Thr Lys Ser Asn Leu Leu Ser His Gln Arg Val Leu Met Gln 130 135 140 Asn Arg Ser Arg Ala Ser Glu Ile Asp Ala Ser Phe Lys Gln Leu Tyr 145 150 155 160 Glu Gln Cys Asn Gly Lys Ser Gly Ala Ser Val Glu Met Lys Asn Trp 165 170 175 Phe Glu Glu Val Met Leu Asn Val Val Thr Arg Asn Val Ser Gly Lys 180 185 190 Arg Ser Phe Gly Thr Lys Ala Arg Leu Gly Asp Ala Glu Ala Val Lys 195 200 205 Tyr Lys Arg Val Ile Asp Glu Thr Ala Arg Phe Met Gly Asn Leu Val 210 215 220 Ile Ser Asp Met Val Pro Ser Leu Trp Trp Leu Asp Asn Met Leu Gly 225 230 235 240 Ala Glu Ser Ala Met Lys Lys Leu Ala Ile Glu Leu Asp Ser Leu Leu 245 250 255 Gly Gly Trp Val Glu Glu His Arg Gly Lys Lys Leu Ser Asp Asp Glu 260 265 270 Glu Gly Asp Phe Ile Ser Leu Thr Trp Glu Met Ile Asp Gln Ile Gln 275 280 285 Leu His Gly Leu Ala Pro Glu Thr Phe Ile Lys Ser Met Cys Val Gly 290 295 300 Val Leu Leu Gly Gly Ser Asp Thr Thr Ser Val Ala Leu Thr Trp Ala 305 310 315 320 Leu Ser Leu Met Leu Asn Asn Arg Glu Val Leu Lys Lys Ala Gln Glu 325 330 335 Glu Leu Asp Phe His Val Gly Lys Asp Arg Lys Val Asp Asp Ser Asp 340 345 350 Leu Lys Asn Leu Val Tyr Leu Gln Ala Ile Met Lys Glu Thr Met Arg 355 360 365 Ile Tyr Gln Val Gly Pro Leu Ile Glu Arg Glu Ala Val Glu Asp Cys 370 375 380 Glu Val Gly Gly Phe Gln Ile Lys Lys Gly Asn Arg Ile Leu Val Asn 385 390 395 400 Leu Trp Lys Leu Gln Arg Asp Pro Glu Val Trp Ser Asp Pro Ser Glu 405 410 415 Phe Arg Pro Glu Arg Phe Leu Glu Asp Lys Ser Lys Val Asp Leu Lys 420 425 430 Gly Gln Asn Ala Glu Leu Met Pro Phe Gly Ser Gly Arg Arg Met Cys 435 440 445 Pro Gly Val Trp Phe Gly Val His Ser Thr Ser Leu Val Leu Ala Arg 450 455 460 Leu Ile Gln Gly Phe Glu Ile Glu Thr Pro Leu Asp Ala Lys Val Asp 465 470 475 480 Met Thr Glu Thr Ser Ser Val Thr Asn Tyr Lys Gly Ser Pro Leu Glu 485 490 495 Val Ile Ala Arg Leu Pro Gly Ser Asn Leu Ser Phe Met Ile Cys Ser 500 505 510 Tyr Val <210> 223 <211> 545 <212> PRT <213> Corydalis chelanthofolia <400> 223 Met Thr Pro Ser Ile Ser Gln Pro Phe Glu Leu Leu Leu Leu Leu Met 1 5 10 15 Thr Met Glu Ser Leu Leu Leu Gln Trp Gln Pro Thr Ser Ile Ala Val 20 25 30 Leu Leu Leu Ala Ile Thr Ile Phe Leu Tyr Thr Phe Thr Lys Ser Pro 35 40 45 Pro Lys Thr His Lys Lys Leu Ala Pro Asp Ala Thr Gly Gly Arg Pro 50 55 60 Leu Met Gly His Leu His Leu Phe Asn Ser Asn Glu Leu Thr His Arg 65 70 75 80 Thr Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Ala Phe Asn Ile Arg 85 90 95 Phe Gly Ser His Lys Thr Leu Val Val Ser Thr Trp Glu Ile Val Lys 100 105 110 Glu Cys Phe Thr Thr Asn Asp Arg Ser Phe Ser Asn Arg Pro Gly Thr 115 120 125 Leu Tyr Ile Lys Leu Met Phe Tyr Asn Glu Asp Ser Val Gly Tyr Ala 130 135 140 Pro Tyr Gly Ala Tyr Trp Arg Asp Leu Arg Lys Leu Ser Thr Leu Lys 145 150 155 160 Leu Leu Ser Asn His Arg Leu Glu Ser Leu Lys His Leu Arg Val Ser 165 170 175 Glu Met Asn Glu Cys Phe Lys Gln Leu Tyr Glu Ile Cys Asn Lys Gly 180 185 190 Ser Lys Ser Val Ala Val Arg Met Asp Asn Trp Phe Gly Asp Leu Thr 195 200 205 Phe Asn Val Val Ala Arg Ile Val Ala Gly Lys Arg Asn Phe Ala Ser 210 215 220 Ile Ser Asn Asp Val Gly Ala Met Arg Tyr Lys Asn Ala Met Asp Glu 225 230 235 240 Ala Phe Arg Leu Met Thr Val Phe Ser Phe Ser Asp Val Ile Pro Ser 245 250 255 Leu Gly Trp Leu Asp Asn Leu Arg Gly Leu Val Gly Asp Met Lys Lys 260 265 270 Ala Gly Lys Glu Ile Asp Ser Val Val Ser Gly Trp Ile Glu Glu His 275 280 285 Arg Glu Lys Arg Lys Arg Arg Ser Ser Gly Gly Asn Asn Arg Asp Gly 290 295 300 Glu Leu Glu Glu Glu Asp Phe Ile Asp Ile Thr Leu Ser Ile Leu Glu 305 310 315 320 Thr Ser Ser Leu Pro Gly Asp Asp Pro Asp Met Thr Ile Lys Ser Thr 325 330 335 Cys Leu Asp Met Ile Leu Gly Gly Ser Asp Thr Thr Thr Val Thr Met 340 345 350 Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Ala His Ala Leu Lys Arg 355 360 365 Ala Arg Glu Glu Leu Asp Leu His Val Gly Arg Asp Arg Gln Val Glu 370 375 380 Asp Ser Asp Ile Asn Asn Leu Val Tyr Ile Gln Ala Ile Ile Lys Glu 385 390 395 400 Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ile Glu Arg Met Thr Lys 405 410 415 Glu Asp Cys Glu Val Gly Gly Phe His Ile Pro Ala Gly Thr Arg Leu 420 425 430 Leu Val Asn Leu Trp Lys Met Gln Arg Asp Pro Asn Val Trp Glu Asp 435 440 445 Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Thr Asn Asn Ala Glu Ile 450 455 460 Asp Leu Lys Gly Gln His Phe Glu Leu Ile Pro Phe Gly Thr Gly Arg 465 470 475 480 Arg Ile Cys Pro Gly Val Ser Phe Ala Leu Gln Leu Met His Leu Ala 485 490 495 Leu Ala Arg Leu Leu His Ser Phe Glu Val Ala Ser Pro Met Asp Ala 500 505 510 Lys Ile Asp Met Thr Glu Thr Ala Gly Leu Ile Ser His Lys Val Ile 515 520 525 Pro Leu Glu Val Leu Met Thr Pro Arg Leu Asp Ser Lys Leu Tyr Asp 530 535 540 Tyr 545 <210> 224 <211> 523 <212> PRT <213> Corydalis chelanthofolia <400> 224 Thr His Thr Thr Glu Lys Asn Leu Pro Pro Glu Val Pro Gly Ala Leu 1 5 10 15 Pro Val Ile Gly His Leu His Lys Leu Gly Gly Arg Lys Gly Leu Leu 20 25 30 Pro Tyr His Val Leu Gly Ala Met Ala Asp Lys Tyr Gly Pro Ala Phe 35 40 45 Thr Phe Gln Phe Gly Ser Asn Arg Thr Leu Val Ile Ser Asn Trp Glu 50 55 60 Met Val Lys Glu Cys Phe Thr Ser Thr Asn Asp Lys Leu Phe Ala Tyr 65 70 75 80 Arg Pro Thr Ala Leu Phe Asn Lys Glu Val Phe Cys Ser Asp His Ser 85 90 95 Phe Gly Phe Gly Pro Tyr Gly Pro Phe Trp Arg Glu Met Arg Lys Val 100 105 110 Cys Ser Leu His Leu Leu Ser Asn Tyr Arg Leu Glu Leu Leu Lys His 115 120 125 Ser Arg Lys Ser Glu Met Asp Glu Cys Phe Lys Glu Leu Tyr Gln Leu 130 135 140 Trp Ser Ala Ser Asn Ile Ser Lys Asp Lys Gln Pro Ala Val Leu Val 145 150 155 160 Val Glu Met Lys Lys Trp Phe Glu Asp Ala Met Phe Asn Val Ala Ser 165 170 175 Arg Val Val Val Gly Lys Gly Ser Leu Lys Ser His Glu Lys Ala Gly 180 185 190 Gly Ser Asp Asn Glu Val Ala Arg Asn Lys Tyr Lys Lys Gly Met Asp 195 200 205 Glu Ala Arg Arg His Met Gly Thr Phe Val Ile Ser Asp Arg Ile Pro 210 215 220 Phe Leu Trp Trp Ile Asp Tyr Leu Arg Gly Ile His Ser Ser Met Lys 225 230 235 240 Arg Thr Ala Lys Asp Leu His Ala Ile Val Glu Thr Trp Leu Glu Glu 245 250 255 His Arg Leu His Cys His Lys Arg Arg Val Leu Gln Leu Asp Ala Asn 260 265 270 His Glu Val Glu Asp Met Ala Lys Glu Glu Glu Lys Asp Phe Met Asp 275 280 285 Val Leu Leu Thr Met Ala Asp Glu Gly Ala Ile Lys Ile Phe Asp His 290 295 300 Asp Leu Asp Thr Val Ile Lys Ala Asn Cys Leu Asp Met Val Ile Ala 305 310 315 320 Gly Thr Asp Thr Pro Thr Val Met Leu Thr Trp Thr Leu Ser Leu Leu 325 330 335 Leu Asn Asn Pro Asp Val Leu Lys Arg Val Arg Asp Glu Leu Asp Leu 340 345 350 His Val Gly Lys Glu Arg Gln Val Glu Glu Ser Asp Val Lys Asn Leu 355 360 365 Val Tyr Leu Gln Ala Val Phe Lys Glu Thr Leu Arg Leu Tyr Pro Ala 370 375 380 Val Pro Leu Asn Glu Arg Leu Thr Met Glu Asp Cys Asn Val Gly Gly 385 390 395 400 Tyr Asp Ile Pro Ala Gly Thr Arg Leu Leu Ile Asn Ile Trp Lys Val 405 410 415 Gln Arg Asp Pro Asn Val Trp Gln Asp Pro Ser Glu Phe Arg Pro Glu 420 425 430 Arg Phe Leu Ser Lys Glu Lys Ser Thr Ile Asp Val Arg Gly Leu Asp 435 440 445 Phe Glu Leu Ile Pro Phe Gly Thr Gly Arg Arg Met Cys Pro Gly Ile 450 455 460 Ser Phe Ser Leu Gln Leu Met His Leu Gly Leu Ala Arg Leu Ile His 465 470 475 480 Gly Phe Glu Leu Ala Thr Pro Met Asp Leu Asp Val Asp Met Ser Ala 485 490 495 Asn Pro Gly Ala Phe Asn Tyr Lys Ser Thr Asp Leu Gln Val Leu Val 500 505 510 Ser Pro Arg Phe His Ser Lys Phe Tyr Gly Cys 515 520 <210> 225 <211> 536 <212> PRT <213> Corydalis chelanthofolia <400> 225 Met Asp Phe Leu Ala Leu Gln Trp Phe Pro Ala Ser Leu Thr Ala Leu 1 5 10 15 Leu Ala Leu Ala Val Leu Tyr Asn Phe Trp Thr Lys Gln Arg Thr Tyr 20 25 30 Lys Asn Gly Lys Thr Ser Thr Lys Gln Ala Pro Val Ala Ala Gly Ala 35 40 45 Trp Pro Val Leu Gly His Leu His Leu Phe Gly Gly Gly Glu Leu Pro 50 55 60 His Lys Met Leu Ala Thr Met Ala Asp Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Met Lys Phe Gly Thr His Gln Thr Leu Val Val Ser Asn Thr Lys Ile 85 90 95 Val Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ala Asn Arg Pro 100 105 110 Ser Thr Thr Ala Phe His Leu Met Thr Tyr Asp Asn Glu Ser Val Ala 115 120 125 Phe Thr Pro Tyr Gly Pro Phe Trp Arg Glu Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ala Ile Lys Asp Val Arg 145 150 155 160 Ala Ser Glu Val Asn Val Cys Phe Arg Glu Leu Tyr Ser Gln Trp Arg 165 170 175 Ile Asn Lys Ser Glu Pro Glu Thr Ser Asp His Gln Gly Pro Ile Leu 180 185 190 Val Asp Met Lys Lys Trp Phe Glu Glu Val Ser Asp Asn Val Val Leu 195 200 205 Arg Val Ile Ala Gly Lys Gln Asn Phe Gly Ser Lys Ile Val His Gly 210 215 220 Asp Lys Glu Ala Leu His Tyr Lys Lys Ile Met Asp Glu Ile Leu Arg 225 230 235 240 Leu Ala Ala Val Ser Met Leu Ser Asp Val Ala Pro Leu Leu Gly Trp 245 250 255 Leu Asp Met Phe Gln Gly His Leu Ser Ala Met Lys Arg Asn Ala Lys 260 265 270 Glu Val Asp Thr Met Leu Cys Asn Trp Leu Glu Glu His Arg Thr Lys 275 280 285 Arg Leu Ser Ser Asp His Asp Gly Val Glu Gln Asp Phe Ile Asp Val 290 295 300 Met Leu Ser Ile Val Glu Glu Asn Lys Phe Ser Gly His Asp Asn Asp 305 310 315 320 Thr Val Ile Lys Ala Thr Val Leu Ala Met Ile Met Gly Gly Thr Asp 325 330 335 Thr Thr Ala Val Ser Leu Thr Trp Ile Val Ser Leu Leu Met Asn Asn 340 345 350 Arg His Val Leu Arg Arg Ala Gln Asp Glu Leu Asp Leu His Val Gly 355 360 365 Lys Asp Arg Gln Val Asp Asp Ser Asp Leu Lys Asn Leu Val Tyr Leu 370 375 380 Asn Ala Ile Val Lys Glu Thr Met Arg Leu Tyr Pro Leu Gly Ala Leu 385 390 395 400 Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu Val Gly Gly Phe His Val 405 410 415 Lys Gly Gly Thr Arg Leu Leu Val Asn Val Trp Lys Leu Gln Arg Asp 420 425 430 Pro Ser Phe Trp Thr Asp Pro Thr Glu Phe Lys Pro Glu Arg Phe Leu 435 440 445 Ala Asp Lys Ala Asn Ile Asp Val Gly Gly Gln His Phe Glu Leu Leu 450 455 460 Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala Leu 465 470 475 480 Gln Phe Met His Leu Val Leu Ala Arg Leu Leu His Gly Phe Asp Leu 485 490 495 Ala Thr Pro Met Asn Ala Asp Val Asp Leu Thr Glu Ser Thr Glu Gly 500 505 510 His Val Asn Gln Lys Ala Ser Pro Leu Asn Leu Leu Val Thr Pro Arg 515 520 525 Leu His Ser Lys Leu Tyr Glu Tyr 530 535 <210> 226 <211> 543 <212> PRT <213> Corydalis chelanthofolia <400> 226 Met Glu Tyr Thr Phe Ser Ser Ala Ser Leu Phe Ser Ser Ser Ser Leu 1 5 10 15 Gln Gln Trp Leu Ser Thr Leu Leu Leu Leu Ile Ser Ser Ile Thr Ile 20 25 30 Ser Leu Tyr Leu Thr Arg Arg Gly Lys Ser Ser Pro Pro Met Val Pro 35 40 45 Gly Ala Trp Pro Leu Ile Gly His Leu His Leu Leu Gly Gly Asn Asp 50 55 60 Pro Leu His Ile Val Leu Gly Thr Met Ala Asp Lys Tyr Gly His Thr 65 70 75 80 Phe Ala Met Leu Phe Gly Lys His Pro Ser Leu Val Val Ser Ser Ser 85 90 95 Asp Ile Val Lys Glu Cys Phe Thr Ser Thr Asn Asp Lys Leu Phe Ser 100 105 110 His Arg His Val Pro Thr Gly Val Lys Tyr Met Phe Tyr Asn Asn Asp 115 120 125 Ser Phe Gly Phe Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Met Arg Lys 130 135 140 Met Asn Ser Leu Ser Leu Phe Ser His His Arg Val Glu Met Leu Asn 145 150 155 160 Arg Ile Arg Thr Ser Gln Ile Asn Ile Trp Phe Lys Asn Leu Tyr Glu 165 170 175 Met Cys Thr Lys Glu Lys Ser Thr Ser Thr Ser Ser Asp Gly Val Val 180 185 190 Val Glu Met Lys Ser Trp Phe Asp Glu Met Phe Phe Asn Val Leu Val 195 200 205 Asn Met Ile Val Lys Pro Ile Asn Asp Asp Glu Glu Leu Val Lys Lys 210 215 220 Tyr Arg Glu Val Ala Thr Glu Ala Gly Arg Leu Leu Ser Ser Met Ala 225 230 235 240 Val Ser Asp Met Val Pro Ser Leu Gly Trp Leu Asp His Leu Phe Gly 245 250 255 Ile Val Gly Lys Met Lys Lys Thr Ala Lys Asp Met Asp Ala Ile Leu 260 265 270 Thr Thr Trp Leu Glu Gln His Lys Ile Lys Ser Gln Gln Leu Arg Arg 275 280 285 Ser Asn Gly Gly Glu Gly Ala Ala Asp Gln Thr Glu Glu Glu Glu Lys 290 295 300 Glu Gly Lys Gly Leu Ile Gly Asp Leu Leu Ser Met Gln Glu Ser Ala 305 310 315 320 Leu Phe Gly His Asp Arg Asp Thr Val Ile Lys Ser Ala Cys Gln Ala 325 330 335 Leu Ile Met Ala Gly Ser Asp Ser Thr Ser Ala Thr Leu Thr Trp Val 340 345 350 Leu Ser Leu Leu Leu Asn His Arg Asp Ala Leu Arg Lys Ala Arg Glu 355 360 365 Glu Leu Asp Gln Val Val Gly Lys Asp Arg Gln Val Asp Asp Ser Asp 370 375 380 Ile Lys Asp Leu Val Tyr Leu Gln Ala Ile Leu Lys Glu Thr Met Arg 385 390 395 400 Leu Tyr Pro Ala Ala Pro Ile Leu Glu Arg Leu Ala Val Glu Asp Cys 405 410 415 Ile Val Gly Gly Phe His Val Gln Ala Gly Thr Thr Leu Phe Val Asn 420 425 430 Val Ser Lys Leu Gln Arg Asp Pro Asn Leu Trp Thr Asp Pro Leu Glu 435 440 445 Phe Lys Pro Glu Arg Phe Leu Thr Gly Cys Asn Ala Asp Val Asp Leu 450 455 460 Lys Gly Gln Asp Tyr Glu Leu Val Pro Phe Gly Ser Gly Arg Arg Ser 465 470 475 480 Cys Pro Ala Val Ser Phe Ala Val Arg Val Met Leu Leu Val Leu Ala 485 490 495 Arg Phe Ile His Ser Phe Glu Val Lys Thr Arg Glu Glu Asp Gly Ile 500 505 510 Leu Asp Met Thr Glu Ser Ser Gly His Thr Asn Cys Arg Ser Ser Pro 515 520 525 Leu Glu Val Leu Ile Ser Pro Arg Leu Asp Ser Lys Ile Tyr Cys 530 535 540 <210> 227 <211> 528 <212> PRT <213> Corydalis chelanthofolia <400> 227 Met Glu Tyr Tyr Ser Ser Leu Pro Phe Leu Gln Gln Trp Pro Leu Ser 1 5 10 15 Ser Ile Ser Ser Ser Pro Thr Ser Ile Ala Thr Thr Leu Leu Ile Val 20 25 30 Ala Cys Phe Thr Ile Val Phe Leu Tyr Ile Asn Thr Ser Ser Lys Ser 35 40 45 Asn Asn Asn Leu Lys Ser Pro Pro Lys Leu Pro Gly Ala Trp Pro Phe 50 55 60 Met Gly His Leu His Leu Leu Gly Gly Asn Gln Pro Ala His Val Ser 65 70 75 80 Leu Ala Asn Ile Ala Glu Lys Tyr Lys Ser Thr Pro Phe Met Ile Arg 85 90 95 Met Gly Gln His Ser Thr Leu Val Val Ser Ser Val Asp Leu Val Lys 100 105 110 Glu Cys Phe Asn Asn Thr Asn Asp Lys Leu Phe Ser Asn Arg Ser Ile 115 120 125 Ala Arg Gly Ile Arg Asp Met Phe Tyr Asp Met Glu Ser Phe Gly Phe 130 135 140 Ala Pro Tyr Gly Tyr Tyr Trp Arg Glu Leu Arg Lys Val Ser Ala Leu 145 150 155 160 Thr Leu Phe Ser Thr His Arg Val Asp Ser Val Ile Gln Ile Lys Thr 165 170 175 Pro Gln Ile Asp Gly Trp Phe Lys Lys Leu Tyr Glu Gln Cys Thr Asn 180 185 190 Thr Lys Gly Gly Ile Val Glu Ile Lys Ser Trp Leu Asp Glu Met Met 195 200 205 Phe Asp Val Leu Val Lys Met Leu Val Glu Pro Lys Asp Glu Gly Ser 210 215 220 Val Glu Lys Tyr Arg His Val Ala Gly Glu Ala Leu Glu Val Leu Gly 225 230 235 240 Asn Met Ser Ile Tyr Asp Leu Val Pro Ser Leu Gly Trp Leu Asp His 245 250 255 Phe Thr Gly Leu Val Lys Lys Tyr Lys Val Thr Gly Lys Lys Met Asp 260 265 270 Thr Ile Leu Asn Ser Trp Leu Glu Asp His Arg Gly Glu Lys Val Ala 275 280 285 Glu Glu His Lys Gly Leu Ile Gly Lys Leu Leu Ser Met Lys Glu Gly 290 295 300 Ser Lys Leu Leu Gly His Asp Gly Asp Thr Ala Ile Lys Ser Thr Val 305 310 315 320 Gln Val Leu Ile Leu Gly Ala Gly Asp Ser Ala Gly Ala Thr Leu Thr 325 330 335 Trp Ala Ile Ser Leu Leu Leu Asn His Pro His Ile Met Glu Lys Leu 340 345 350 Arg Lys Glu Leu Asp Ala Ala Val Gly Lys Asp Arg Gln Ile Glu Asp 355 360 365 Ser Asp Val Lys Lys Phe Thr Tyr Leu Leu Ala Ile Leu Lys Glu Thr 370 375 380 Met Arg Leu Tyr Pro Val Thr Thr Leu Leu Gln Arg Glu Thr Met Glu 385 390 395 400 Asp Cys Ala Ile Gly Gly Tyr His Val Ala Ala Gly Thr Arg Leu Met 405 410 415 Val Asn Val Trp Gln Val Gln Arg Asp Pro Asn Ala Trp Ser Asp Pro 420 425 430 Leu Glu Phe Lys Pro Glu Arg Phe Leu Thr Glu Cys Ala His Val Asp 435 440 445 Phe Ser Gly Gln Tyr Ser Glu Leu Ile Pro Phe Gly Thr Gly Arg Arg 450 455 460 Ile Cys Pro Ala Leu Thr Leu Ser Val Arg Leu Met Leu Leu Thr Leu 465 470 475 480 Ala Arg Ile Ile His Ser Phe Asp Val Lys Ile Pro Asn Gly Ala Ser 485 490 495 Ser Val Asp Met Ala Thr Ser Val Gly Phe Val Asn Leu Arg Leu Thr 500 505 510 Pro Leu Glu Val Glu Leu Ser Pro Arg Leu Asp Ser Lys Ile Tyr Gly 515 520 525 <210> 228 <211> 537 <212> PRT <213> Corydalis chelanthofolia <400> 228 Met Asp Ser Ser Leu Leu Gln Trp Tyr Thr Thr Ala Ser Met Ala Ala 1 5 10 15 Leu Leu Ala Leu Ala Phe Phe Tyr Lys Leu Trp Ser Lys Pro Arg Thr 20 25 30 Ser Lys Asn Gly Lys Ser Thr Leu Gln Ala Pro Val Ala Ala Gly Ala 35 40 45 Trp Pro Val Leu Gly His Leu His Leu Phe Ser Gly Gly Glu Leu Pro 50 55 60 His Lys Met Leu Ala Ala Met Ala Asp Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Met Lys Phe Gly Thr His Arg Thr Leu Val Val Ser Asp Thr Lys Ile 85 90 95 Val Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ala Asn Arg Pro 100 105 110 Ser Thr Met Ala Phe His Leu Met Thr Tyr Asp Asn Glu Ser Val Ala 115 120 125 Phe Thr Pro Tyr Gly Gln Phe Trp Arg Glu Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ala Ile Lys Asp Val Arg 145 150 155 160 Ala Ser Glu Val Asn Phe Cys Phe Arg Thr Leu Tyr Asn Gln Trp Arg 165 170 175 Ile Asn Lys Ser Glu Thr Ile Gly Thr Gly Asp His Gln Gly Pro Ile 180 185 190 Leu Val Asp Met Lys Lys Trp Phe Glu Glu Val Ser Asn Asn Val Val 195 200 205 Ile Arg Val Ile Val Gly Lys His Asn Phe Gly Ser Lys Ile Ala Arg 210 215 220 Gly Lys Gly Glu Ala Leu His Tyr Lys Lys Val Met Asp Glu Val Leu 225 230 235 240 Arg Leu Ala Ala Val Ser Met Leu Ser Asp Val Ala Pro Leu Leu Gly 245 250 255 Trp Phe Asp His Leu Gln Gly His Val Ser Ala Met Lys Arg Asn Ala 260 265 270 Lys Glu Val Asp Thr Leu Leu Cys Ser Trp Met Glu Glu His Arg Lys 275 280 285 Lys Arg Thr Ser Asn Gly Ser Asn Gly Val Glu Gln Asp Phe Ile Asp 290 295 300 Val Met Leu Ser Ile Val Glu Glu Asn Lys Phe Ser Gly His Asp Ser 305 310 315 320 Asp Thr Val Ile Lys Ala Thr Val Leu Ala Met Ile Met Gly Gly Thr 325 330 335 Asp Thr Ser Ala Val Ser Leu Thr Trp Ile Val Ser Leu Leu Met Asn 340 345 350 Asn Arg His Val Leu Arg Arg Ala Gln Glu Glu Leu Asp Thr His Val 355 360 365 Gly Lys Asp Arg Gln Val Asp Asp Ser Asp Leu Lys Asn Leu Val Tyr 370 375 380 Leu Asn Ala Ile Val Lys Glu Thr Met Arg Leu Tyr Pro Leu Gly Ala 385 390 395 400 Leu Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu Val Gly Gly Phe His 405 410 415 Val Lys Ala Gly Thr Arg Leu Leu Val Asn Val Trp Lys Leu Gln Arg 420 425 430 Asp Pro Asn Phe Trp Ile Asp Pro Thr Glu Phe Lys Pro Glu Arg Phe 435 440 445 Leu Thr Asp Asn Ala Asn Ile Asp Val Gly Gly Gln His Phe Glu Leu 450 455 460 Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala 465 470 475 480 Leu Gln Phe Met His Leu Val Leu Ala Arg Leu Ile His Gly Phe Glu 485 490 495 Leu Asp Thr Pro Met Asn Ala Asn Val Asp Met Thr Glu Ser Thr Glu 500 505 510 Gly His Val Asn His Lys Ala Ser Ala Leu Asp Leu Leu Ile Thr Pro 515 520 525 Arg Leu Arg Ser Thr Leu Tyr Asp Tyr 530 535 <210> 229 <211> 571 <212> PRT <213> Corydalis chelanthofolia <400> 229 Met Phe Lys Ser Val Pro Ser Ala Gln Ala Ile Leu His Lys Leu Ser 1 5 10 15 Phe Ile His Gln Tyr Phe Leu Leu Ser Gln Thr Asn Met Asp Ser Leu 20 25 30 Leu Phe Gln Trp Phe Pro Ala Ser Met Ala Ala Leu Val Ala Phe Ala 35 40 45 Phe Leu Tyr Lys Leu Trp Ser Ala Pro Lys Ile Thr Lys Asn Gly Lys 50 55 60 Thr Ser Thr Lys Gln Ala Pro Ile Ala Ala Gly Ala Trp Pro Val Leu 65 70 75 80 Gly His Leu His Leu Phe Gly Gly Gly Glu Leu Pro His Lys Met Leu 85 90 95 Ala Gly Met Ala Glu Lys Tyr Gly Pro Ala Phe Thr Met Lys Phe Gly 100 105 110 Met His Arg Thr Leu Val Val Ser Asp Thr Lys Ile Val Lys Glu Cys 115 120 125 Phe Thr Thr His Asp Thr Leu Phe Ala Asn Arg Pro Ser Thr Thr Ala 130 135 140 Phe His Leu Met Thr Tyr Asp Gln Glu Ser Val Ala Phe Thr Pro Tyr 145 150 155 160 Gly Pro Phe Trp Arg Glu Leu Arg Lys Ile Ser Thr Leu Lys Leu Leu 165 170 175 Ser Asn His Arg Leu Gln Ala Ile Lys Asp Val Arg Thr Ser Glu Val 180 185 190 Asn Val Cys Phe Arg Gly Leu Tyr Asn Ile Trp Gln Thr Asn Lys Asn 195 200 205 Glu Gln Gly Gly Thr Val Ser Asp His Pro Arg Pro Val Leu Ile Asp 210 215 220 Met Lys Lys Trp Phe Glu Glu Val Ser Asn Asn Val Val Ile Arg Val 225 230 235 240 Ile Ala Gly Lys Gln Asn Phe Gly Ser Lys Ile Val Arg Gly Glu Glu 245 250 255 Glu Ala Val Lys Tyr Lys Lys Ile Met Asp Glu Val Leu Arg Leu Ala 260 265 270 Ala Val Ser Met Leu Ser Asp Val Ala Pro Leu Leu Gly Trp Leu Asp 275 280 285 Leu Phe Gln Gly Asn Leu Ser Ala Met Lys Arg Asn Ala Lys Glu Val 290 295 300 Asp Asn Met Leu Asn Ala Trp Val Glu Glu His Arg Arg Lys Arg Leu 305 310 315 320 Ala His Ser Ala Lys Val Glu Ala Thr His Val Glu Gln Asp Phe Val 325 330 335 Asp Val Met Leu Ser Ile Met Glu Glu Asn Lys Leu Ser Ser Val His 340 345 350 Asp Asp Asp Thr Val Ile Lys Ala Thr Val Leu Ala Met Ile Met Gly 355 360 365 Gly Thr Asp Thr Thr Ala Val Ser Leu Thr Trp Thr Val Ser Leu Leu 370 375 380 Met Asn His Arg Tyr Val Leu Lys Lys Ala Gln Glu Glu Ile Asp Gln 385 390 395 400 His Val Gly Lys Glu Arg Gln Val Glu Asp Ser Asp Leu Lys Asn Leu 405 410 415 Val Tyr Leu Asn Ala Ile Val Lys Glu Thr Met Arg Met Tyr Pro Leu 420 425 430 Gly Ala Leu Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu Val Gly Gly 435 440 445 Phe His Val Lys Gly Gly Thr Arg Leu Leu Val Asn Val Trp Lys Leu 450 455 460 Gln Arg Asp Pro Asn Val Trp Ile Asp Pro Thr Glu Phe Lys Pro Glu 465 470 475 480 Arg Phe Leu Ala Glu Asn Ala Asn Ile Asp Val Gly Gly Gln His Phe 485 490 495 Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val Ser 500 505 510 Phe Ala Leu Gln Phe Met His Leu Val Leu Ala Arg Leu Ile His Gly 515 520 525 Phe Glu Leu Glu Thr Pro Met Asn Ala Asp Val Asp Leu Thr Glu Ser 530 535 540 Thr Glu Gly His Val Asn His Lys Ala Ser Pro Leu Asp Leu Leu Ile 545 550 555 560 Ala Pro Arg Leu Asn Ser Lys Leu Tyr Glu Leu 565 570 <210> 230 <211> 533 <212> PRT <213> Corydalis chelanthofolia <400> 230 Met Asp Ser Leu Phe Val Leu Leu Gln Trp Phe Ser Ala Ser Leu Ala 1 5 10 15 Ala Val Leu Ala Leu Ala Phe Leu Tyr Asn Leu Trp Val Lys Pro Arg 20 25 30 Ile Ser Asp Gly Lys Gly Asn Lys Gly Ser Thr Lys Gln Ala Pro Val 35 40 45 Ala Ala Gly Ala Trp Pro Val Leu Gly His Leu His Leu Phe Gly Gly 50 55 60 Ser Glu Leu Pro His Lys Met Leu Ala Ala Met Ala Asp Lys Tyr Gly 65 70 75 80 Pro Ala Phe Thr Met Lys Phe Gly Thr His Arg Thr Leu Val Val Ser 85 90 95 Asn Thr Gln Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Thr His Phe 100 105 110 Ser Asn Arg Pro Ser Thr Thr Ala Phe His Leu Met Thr Tyr Asp Asn 115 120 125 Asp Ser Val Ala Phe Thr Pro Tyr Gly Pro Phe Trp Arg Glu Leu Arg 130 135 140 Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ala Ile 145 150 155 160 Lys Asp Val Arg Val Ser Glu Val Asn Val Cys Phe Lys Asp Leu Tyr 165 170 175 Asn Gln Trp Lys Thr Asn Gln Asn Gln Arg Pro Val Leu Val Asp Met 180 185 190 Lys Lys Trp Phe Glu Glu Val Ser Asn Asn Val Val Ile Arg Val Ile 195 200 205 Val Gly Lys Gln Asn Phe Gly Ser Lys Ile Val Arg Gly Glu Glu Glu 210 215 220 Ala Val Lys Tyr Lys Lys Ile Met Asp Glu Ile Leu Arg Leu Ala Ala 225 230 235 240 Val Pro Met Leu Ser Asp Met Ala Pro Leu Leu Gly Trp Leu Asp Leu 245 250 255 Phe Gln Ala His Lys Ser Ala Met Lys Arg Asn Ala Lys Glu Val Asp 260 265 270 Asn Met Leu Glu Ser Trp Leu Glu Glu His Lys Arg Lys Arg Leu Ser 275 280 285 Gly Val Asn Gly Ala Glu Glu Glu Asp Phe Met Asp Val Met Leu Ser 290 295 300 Ile Met Glu Glu Asn Lys Phe Thr Gly Arg Asp Ser Asn Thr Val Val 305 310 315 320 Lys Ser Thr Val Leu Ala Met Ile Met Gly Gly Thr Asp Thr Thr Ala 325 330 335 Val Ser Leu Thr Trp Ile Phe Ser Leu Leu Met Asn Asn Arg His Ala 340 345 350 Leu Lys Lys Ala Gln Glu Glu Leu Asp Met His Val Gly Lys Asp Arg 355 360 365 Gln Val Glu Asp Ser Asp Leu Lys Asn Leu Val Tyr Leu Asn Ala Ile 370 375 380 Val Lys Glu Thr Met Arg Met Tyr Pro Leu Gly Ala Leu Leu Glu Arg 385 390 395 400 Glu Thr Lys Glu Asp Cys Glu Val Gly Gly Tyr His Val Arg Ala Gly 405 410 415 Thr Arg Leu Leu Val Asn Val Trp Lys Leu Gln Arg Asp Pro Ser Phe 420 425 430 Trp Thr Asp Pro Thr Glu Phe Lys Pro Glu Arg Phe Leu Thr Glu Asn 435 440 445 Ala Lys Thr Asp Val Gly Gly Gln His Phe Glu Leu Leu Pro Phe Gly 450 455 460 Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala Leu Gln Phe Met 465 470 475 480 His Leu Val Ile Ala Arg Leu Ile His Gly Phe Glu Leu Gly Thr Pro 485 490 495 Met Asp Ala Asp Val Asp Leu Thr Glu Ser Thr Glu Gly His Val Asn 500 505 510 His Lys Ala Thr Pro Leu Asp Leu Leu Ile Ala Pro Arg Leu Asp Ser 515 520 525 Lys Val Tyr Asp Tyr 530 <210> 231 <211> 530 <212> PRT <213> Chelidonium majus <400> 231 Met Arg Ser Asn Tyr Leu Lys Pro Thr Thr Thr Thr Ile Glu Asp Lys 1 5 10 15 Ser Lys Asn Lys Ile Ser Arg Lys Lys Pro Ile Ala Ala Lys Pro Pro 20 25 30 Pro Glu Ala Leu Gly Ala Trp Pro Val Ile Gly His Phe Leu Leu Phe 35 40 45 Thr Gly Lys Gly Leu Thr His Val Thr Leu Gly Asp Met Ala Asp Lys 50 55 60 Tyr Gly Pro Ala Phe Leu Ile Arg Phe Gly Ser His Arg Thr Leu Val 65 70 75 80 Val Ser Ser Trp Glu Met Met Lys Glu Cys Phe Ser Ala Pro Asn Asp 85 90 95 Lys Ile Phe Ser Asn Arg Glu Ser Asn Leu Leu Trp Ile Lys Ser Met 100 105 110 Phe Tyr Gly Ser Asn Ser Tyr Gly Phe Ser Pro Tyr Gly Pro Tyr Trp 115 120 125 Lys Glu Leu Arg Lys Ile Ser Thr Gln Lys Leu Leu Ser His His Arg 130 135 140 Leu Asp Ala Met Lys His Leu Gln Met Val Glu Val Asp Ala Ser Phe 145 150 155 160 Lys Gln Ile Tyr Asn Leu Cys Asn Lys Asn Gly Gly Ser Pro Ser Pro 165 170 175 Ser Ser Ile Asn Thr Thr Ala Leu Val Asn Met Asp Glu Trp Leu Ser 180 185 190 His Leu Met Phe Asn Val Ile Ala Arg Met Val Ser Gly Tyr Gln Ser 195 200 205 Asp Asp Val Gly Thr Gly Ala Thr Ser Thr Gly Glu Arg Phe Lys Val 210 215 220 Ser Met Asp Glu Thr Met Arg Leu Met Ala Ile Phe Ala Val Ser Asp 225 230 235 240 Leu Val Pro Trp Leu Ala Cys Val Asp Arg Leu Arg Gly Leu Thr Arg 245 250 255 Lys Met Asn Arg Cys Gly Lys Asp Leu Asp Ser Ile Ile Gly Arg Leu 260 265 270 Ile Glu Glu His Arg Gln Lys Arg Arg Leu Cys Arg Asn Asn Lys Gly 275 280 285 Ser Lys Asn Glu Asp Gly Gly His Asp Asp Asp Gln Asp Phe Ile Asp 290 295 300 Ile Cys Leu Ser Ile Met Glu Gln Ser Gln Leu Pro Gly Asp Asn Pro 305 310 315 320 Glu Ile Ala Ile Lys Ser Ile Val Leu Asp Met Ile Phe Ala Gly Ser 325 330 335 His Thr Thr Thr Leu Ala Met Thr Trp Thr Leu Ser Leu Leu Leu Asn 340 345 350 His Arg His Met Leu Lys Lys Ala Lys Glu Glu Ile Asp Ala His Val 355 360 365 Gly Asn Glu Arg Gln Val Asp Asp Ser Asp Ile His Asn Leu Val Tyr 370 375 380 Ile Gln Ala Ile Ile Lys Glu Ser Met Arg Leu Tyr Pro Pro Ser Pro 385 390 395 400 Leu Phe Glu Arg Met Thr Met Glu Asp Cys Glu Val Gly Gly Phe His 405 410 415 Ile Pro Ala Gly Thr Arg Leu Phe Val Asn Val Trp Lys Met Gln Arg 420 425 430 Asp Pro Thr Val Trp Glu Asp Pro Leu Glu Phe Arg Pro Glu Arg Phe 435 440 445 Leu Thr Ser Lys Arg Glu Ile Asp Val Lys Gly Gln His Tyr Glu Leu 450 455 460 Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ala Ser Phe Thr 465 470 475 480 Leu Gln Val Leu His Leu Val Leu Ala Arg Leu Ile His Gly Phe Glu 485 490 495 Met Thr Thr Pro Met Asp Val Lys Val Asp Met Ala Ser Ser Ala Gly 500 505 510 Leu Phe Ser Asn Lys Met Thr Pro Leu Glu Val Leu Ile Thr Pro Arg 515 520 525 Thr Ala 530 <210> 232 <211> 557 <212> PRT <213> Chelidonium majus <400> 232 Met Lys Ile Leu Gln Glu Phe His Gln Leu Thr Ile Ser Asn Ile Val 1 5 10 15 Leu Leu Leu Leu Ile Ser Phe Ile Phe Phe Ser Leu Ser Ala Trp Phe 20 25 30 Ile Glu Ser Thr Asn Tyr Tyr Tyr Ser Ser Lys Lys Lys Lys Ser Lys 35 40 45 Lys Thr Pro Pro Pro Glu Ala Ser Gly Ala Trp Pro Val Ile Gly His 50 55 60 Leu His Gln Phe Lys Pro Asp Asp Leu Pro His Glu Ala Leu Gly Asp 65 70 75 80 Met Ala Asp Lys Tyr Gly Pro Val Phe Ile Val Arg Phe Gly Ser Tyr 85 90 95 Arg Lys Leu Val Val Ser Asn Ser Glu Met Val Lys Glu Cys Phe Thr 100 105 110 Ala Ala Asn Asp Arg Ser Phe Ser Asn Arg Pro Ala Val Leu Gly Ile 115 120 125 Arg Ile Met Leu Tyr Asn Thr Ile Thr Tyr Ala Phe Ala Pro Tyr Gly 130 135 140 Pro Tyr Trp Arg Glu Leu Arg Arg Ile Ser Ser Gln Asn Leu Leu Ser 145 150 155 160 Asn His Arg Val Asp Met Val Lys His Leu His Val Asp Glu Val Asn 165 170 175 Thr Leu Phe Lys Gln Leu Tyr Glu Leu Cys Asn Lys Asn Gly Gly Arg 180 185 190 Pro Pro Thr Gly Thr Asn Thr Ser Thr Ser Ala Ala Leu Val Asn Met 195 200 205 Asp Asp Trp Leu Ser Asn Ile Met Phe Asn Val Ile Ala Arg Ile Val 210 215 220 Asn Gly Asn Asn Lys Ser Ile Asn Asn Glu Arg Thr Gly Ala Ile Asn 225 230 235 240 Glu Lys Arg Tyr Lys Thr Ala Met Asp Glu Ala Arg Arg Leu Val Ala 245 250 255 Ile Phe Ala Val Ser Asp Ser Val Pro Trp Leu Gly Trp Leu Asp Arg 260 265 270 Val Arg Gly Leu Ile Arg Gly Met Asn Leu Cys Gly Lys Glu Leu Asp 275 280 285 Ser Ile Ile Glu Asp Ile Ile Asp Glu His Arg Gln Lys Arg Arg Leu 290 295 300 Cys Thr Ser Lys Leu Gly Ser Ser Gly Asp Asp Asp Ala Asp Asn Ser 305 310 315 320 Asn Leu Glu Glu Glu Asp Asn Phe Ile Asp Ala Cys Leu Ser Ile Glu 325 330 335 Glu Gln Ser Gln Leu Pro Gly Glu Asn Pro Glu Ile Val Ile Lys Ala 340 345 350 Leu Ile Leu Asp Met Phe Ala Gly Gly Ser Asp Gly Pro His Phe Val 355 360 365 Leu Thr Trp Thr Leu Ala Leu Leu Leu Asn His Pro His Val Leu Lys 370 375 380 Lys Ala Arg Glu Glu Val Asp Ala His Val Ala Asn Asp Arg His Val 385 390 395 400 Asp Val Ser Asp Ile Ser Asn Leu Val Tyr Ile Gln Ala Ile Leu Lys 405 410 415 Glu Ser Met Arg Leu Tyr Pro Pro Asn Ala Leu Ile Glu Arg Met Thr 420 425 430 Thr Glu Asp Cys Glu Val Gly Gly Tyr His Ile Pro Ala Gly Thr Tyr 435 440 445 Leu Leu Val Asn Val Trp Lys Val Gln Arg Asp Pro Thr Val Trp Gln 450 455 460 Asp Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Leu Thr Asn Thr Lys 465 470 475 480 Thr Glu Met Glu Tyr Glu His Ile Pro Phe Gly Ala Gly Arg Arg Arg 485 490 495 Cys Pro Gly Met Arg Phe Ala Leu Gln Val Met His Leu Val Leu Ala 500 505 510 Arg Leu Ile His Glu Phe Glu Ile Thr Thr Pro Val Asp Val Ile Lys 515 520 525 Val Asp Met Thr Ala Thr Asn Gly Leu Phe Asn His Lys Ala Val Pro 530 535 540 Leu Glu Val Leu Leu Thr Pro Arg Thr Leu Ile Arg Gly 545 550 555 <210> 233 <211> 541 <212> PRT <213> Chelidonium majus <400> 233 Met Asp Tyr Ser Val Leu Leu Gln Tyr Gly Trp Phe Ala Pro Ser Met 1 5 10 15 Ala Ala Leu Leu Ala Leu Ala Phe Leu Tyr Asn Leu Phe Leu Ala Ser 20 25 30 Ser Pro Lys Ala Thr Ser Lys Arg Thr Ile Ser Thr Lys Lys Pro Pro 35 40 45 Met Ala Ala Gly Ala Trp Pro Ile Leu Gly His Leu His Leu Phe Lys 50 55 60 Glu Gly Glu Leu Pro His Gln Met Leu Lys Ser Met Ala Asp Lys Tyr 65 70 75 80 Gly Pro Ala Phe Leu Met Lys Phe Gly Gln His Arg Ser Leu Val Val 85 90 95 Ser Asp Tyr Arg Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Thr His 100 105 110 Phe Cys Asn Arg Pro Ser Thr Thr Ala Phe Asp Val Met Thr Tyr Ala 115 120 125 Asn Glu Ser Val Ala Phe Thr Glu Tyr Ser Pro Tyr Trp Arg Glu Leu 130 135 140 Arg Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn Asn Arg Leu Gln Ala 145 150 155 160 Ile Lys Asn Leu Arg Glu Glu Glu Val Asn Val Ser Phe Lys Gly Leu 165 170 175 Tyr Asp Ser Trp Lys Asn Lys Asn Asn Lys Ser Thr Gly Ser Val Gly 180 185 190 Asp Glu Arg Ala Pro Val Leu Val Asp Met Lys Lys Trp Phe Glu Glu 195 200 205 Val Ser Asn Asn Val Val Ile Arg Val Ile Val Gly Lys Cys Asn Phe 210 215 220 Gly Thr Lys Ile Val Gln Gly Glu Lys Glu Gly Val Glu Tyr Lys Thr 225 230 235 240 Ile Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Leu Leu Ser Asp 245 250 255 Phe Ala Pro Ile Leu Gly Leu Leu Asp Phe Phe Gln Gly His Val Arg 260 265 270 Thr Met Lys Arg Asn Gly Lys Lys Leu Asp Val Leu Leu Gln Arg Trp 275 280 285 Leu Glu Glu His Lys Arg Lys Lys Ser Thr Pro Glu Asp Glu Gln Asp 290 295 300 Phe Met Asp Val Met Leu Ser Val Ile Glu Glu Ser Lys Leu Ser Gly 305 310 315 320 Tyr Asp Ala Asp Thr Val Ile Lys Ala Thr Cys Leu Ala Met Ile Met 325 330 335 Gly Gly Thr Asp Thr Ser Ala Val Ser Leu Thr Trp Ile Val Ser Leu 340 345 350 Leu Met Asn Asn Arg His Ala Leu Lys Lys Ala Arg Glu Glu Leu Asp 355 360 365 Ala Gln Val Gly Lys Asp Arg Gln Val Glu Asp Ser Asp Leu Lys Asn 370 375 380 Leu Val Tyr Leu Asn Ala Ile Val Lys Glu Thr Met Arg Leu Tyr Pro 385 390 395 400 Leu Gly Thr Leu Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu Val Gly 405 410 415 Gly Phe His Leu Glu Gly Gly Thr Arg Leu Leu Val Asn Val Trp Met 420 425 430 Val Gln Arg Asp Pro Ser Val Trp Thr Asp Pro Thr Lys Phe Thr Pro 435 440 445 Glu Arg Phe Leu Thr Glu Lys Ala Asp Ile Asp Val Trp Gly Gly Asn 450 455 460 Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val 465 470 475 480 Ser Phe Ala Leu Gln Phe Leu Asn Leu Val Leu Ala Arg Leu Ile His 485 490 495 Gly Tyr Glu Leu Gly Thr Pro Asp Asp Ala Asp Val Asp Leu Thr Glu 500 505 510 Ser Pro Glu Gly His Leu Asn His Lys Ala Ser Pro Leu Glu Leu Leu 515 520 525 Leu Thr Pro Arg Leu Ser Asn Pro Lys Leu Tyr Asp Tyr 530 535 540 <210> 234 <211> 537 <212> PRT <213> Chelidonium majus <400> 234 Met Glu Phe Leu Ser Leu Glu Pro Gln Leu Ile Ser Ile Phe Val Leu 1 5 10 15 Leu Leu Ala Ser Ser Ile Phe Leu Tyr Asn Leu Leu Lys Asn His Gly 20 25 30 Arg Lys Ser Lys Thr Ser Lys Pro Pro Ala Pro Val Ala Ser Gly Gly 35 40 45 Trp Pro Ile Met Gly His Leu His Leu Phe Asn Gly Ser Glu Leu Thr 50 55 60 His Gln Thr Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Ala Phe Asn 65 70 75 80 Ile Gln Leu Gly Ser His Gln Thr Leu Val Val Ser Ser Trp Glu Ile 85 90 95 Val Lys Glu Cys Phe Thr Thr Asn Asp Arg Phe Phe Ser Asn Arg Pro 100 105 110 Gly Ser Leu Ala Ile Lys Leu Met Phe Tyr Asp Ala Asp Ser Val Gly 115 120 125 Tyr Ala Pro Tyr Gly Ala Tyr Trp Arg Asp Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn His Arg Val Glu Thr Leu Lys His Leu Arg 145 150 155 160 Thr Ser Glu Ala Glu Ser Cys Phe Lys Gln Leu Tyr Asn Gln Trp Lys 165 170 175 Asn Asn Lys Val Gly Gly Asp Asp Asp Glu Phe Ala Ile Val Arg Met 180 185 190 Asp Asn Trp Phe Gly Asp Leu Thr Phe Asn Val Val Ala Arg Ile Val 195 200 205 Ala Gly Lys Lys Asn Phe Ala Gly Gly Ala Thr Ser Gly Asp Val Gly 210 215 220 Ala Lys Arg Tyr Lys Glu Ala Met Asp Glu Ala Phe Arg Leu Met Thr 225 230 235 240 Ile Phe Ala Phe Ser Asp Val Val Pro Ser Leu Gly Trp Leu Asp Lys 245 250 255 Leu Arg Gly Leu Val Gly Gly Met Lys Arg Cys Gly Ala Glu Ile Asp 260 265 270 Ser Ile Val Gly Gly Trp Val Asp Glu His Arg Leu Lys Arg Ala Ser 275 280 285 Arg Lys Gly Gly Asp His Asn Asp Leu Asp Leu Glu Gln Asp Phe Ile 290 295 300 Asp Val Cys Leu Glu Ile Met Glu His Ser Thr Leu Pro Gly Asp Asp 305 310 315 320 Pro Glu Ile Val Ile Lys Ser Thr Cys Leu Asp Met Ile Leu Gly Gly 325 330 335 Ser Asp Thr Thr Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu 340 345 350 Asn Asn Pro His Val Leu Lys Arg Ala Arg Glu Glu Leu Asp Thr His 355 360 365 Val Gly Lys Asp Arg Gln Val Asp Asp Ser Asp Met Ser Asn Leu Val 370 375 380 Tyr Ile Gln Ala Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly 385 390 395 400 Pro Leu Ile Glu Arg Arg Thr Ser Glu Asp Cys Glu Val Ser Gly Phe 405 410 415 His Val Pro Ala Gly Thr Arg Leu Met Val Asn Leu Trp Lys Met Gln 420 425 430 Arg Asp Gly Ser Val Tyr Lys Glu Asp Pro Leu Glu Phe Arg Pro Glu 435 440 445 Arg Phe Leu Thr Ser Asn Ala Asp Val Asp Leu Lys Gly Gln Asn Tyr 450 455 460 Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser 465 470 475 480 Phe Ala Val Gln Leu Met His Leu Val Leu Ala Arg Leu Val His Gly 485 490 495 Phe Glu Met Lys Thr Ala Gly Gly Ala Lys Val Asp Met Thr Glu Ser 500 505 510 Ala Gly Leu Ile Ser His Lys Val Thr Pro Leu Gln Val Leu Leu Lys 515 520 525 Pro Arg Leu Ala Ile Gln Gln Ala Leu 530 535 <210> 235 <211> 550 <212> PRT <213> Chelidonium majus <400> 235 Met Asp Ser Met Asn Leu Phe Gln Gln Ala Ile Ala Val Gly Thr Leu 1 5 10 15 Val Ile Phe Leu Tyr Tyr Leu Trp Lys Trp Thr Ser Ile Ile Phe Thr 20 25 30 Arg Thr His Gln Ala Ala Pro Pro Glu Pro Ala Gly Ala Trp Pro Ile 35 40 45 Ile Gly His Leu His Leu Leu Ala Gly Gly Ala Asn Gln Leu Val Tyr 50 55 60 His Ile Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Ile Phe Thr Val 65 70 75 80 Arg Leu Gly Met Arg Arg Ala Leu Ile Val Ser Asn Ser Glu Leu Ala 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Arg Ile Phe Ser Thr Arg Pro Ser 100 105 110 Ser Val Ala Ile Lys Leu Met Gly Tyr Asp Ser Ala Met Phe Gly Phe 115 120 125 Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Met Arg Lys Ile Ala Val Thr 130 135 140 Glu Leu Leu Ser Asn Arg Arg Leu Glu Ile Leu Lys Asn Ile Arg Ile 145 150 155 160 Ser Glu Ile Asn Met Ser Ile Lys Asp Leu Tyr Gln Leu Trp Val Glu 165 170 175 Asn Lys Asn Ser Gly Val Asp Gly Gly Gly Asp Gly Gly Glu Val Val 180 185 190 Val Gly Ser Pro Val Leu Val Glu Met Lys Arg Trp Phe Glu Asp Val 195 200 205 Ser Phe Asn Val Val Val Arg Met Val Ala Gly Lys Arg Tyr Phe Gly 210 215 220 Arg Arg Val Glu Ser Asp Asp Arg Glu Glu Glu Glu Ala Arg Arg Trp 225 230 235 240 Gln Lys Ala Met Asn Asp Phe Met His Leu Val Gly Ile Phe Val Val 245 250 255 Ser Asp Ala Ile Pro Phe Leu Gly Phe Leu Asp Phe Gln Gly Tyr Glu 260 265 270 Lys Glu Met Lys Lys Thr Ala Val Glu Ile Asp Tyr Val Met Gly Arg 275 280 285 Trp Val Glu Glu His Gln Arg Arg Lys Leu Glu Arg Val Ala Gly Gly 290 295 300 Ser Asp Asp Gln Gln Glu Asp Phe Ile Asp Val Met Leu Ser Ile Leu 305 310 315 320 Lys Asp Gly Asp Gln Phe Tyr Gly Tyr Asp Pro Asp Thr Val Ile Lys 325 330 335 Ser Ser Cys Leu Ser Leu Val Leu Gly Gly Ser Asp Thr Ile Ser Val 340 345 350 Thr Leu Thr Trp Thr Val Ser Leu Leu Leu Asn Asn Arg Asn Val Leu 355 360 365 Glu Lys Ala Gln Ser Glu Leu Asp Ile His Val Gly Arg Gly Arg Gln 370 375 380 Val Asp Glu Ser Asp Ile Asn Asn Leu Lys Tyr Leu Gln Ala Ile Val 385 390 395 400 Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ser Ala Pro Arg 405 410 415 Glu Ala Met Glu Asp Cys Thr Ile Ala Gly Phe His Val Pro Lys Gly 420 425 430 Thr Gln Leu Met Leu Asn Leu Trp Lys Leu His Arg Asp Pro Arg Val 435 440 445 Trp Ser Asp Pro Leu Glu Phe Lys Pro Glu Arg Phe Leu Thr Thr Gly 450 455 460 Thr His Gly Asp Val Asp Val Lys Gly Gln His Phe Glu Leu Leu Pro 465 470 475 480 Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Ile Ser Tyr Ala Leu Gln 485 490 495 Val Leu His Leu Thr Leu Ala Arg Leu Leu His Ser Phe His Leu Ser 500 505 510 Thr Pro Tyr Asp Ile Pro Val Asp Met Thr Glu Ser Ser Gly Leu Ser 515 520 525 Cys Pro Lys Ala Thr Pro Leu Asp Val Leu Leu Thr Pro Arg Leu Pro 530 535 540 Ser Glu Leu Tyr Val Ser 545 550 <210> 236 <211> 561 <212> PRT <213> Chelidonium majus <400> 236 Met Asp Leu Phe Ile Phe Phe Ser Arg Phe Gln Tyr Ile Val Gly Leu 1 5 10 15 Leu Ala Phe Leu Thr Phe Phe Tyr Tyr Leu Trp Arg Val Ser Ile Thr 20 25 30 Gly Thr Arg Ile Lys Thr Asn Gln Asn Ile Met Asn Gly Thr Asn Met 35 40 45 Met Ala Pro Glu Ala Ala Gly Ala Trp Pro Ile Val Gly His Leu Pro 50 55 60 Gln Leu Val Gly Pro Gln Pro Leu Phe Lys Ile Leu Gly Asp Met Ala 65 70 75 80 Asp Lys Tyr Gly Ser Ile Phe Met Val Arg Phe Gly Met His Pro Thr 85 90 95 Leu Val Val Ser Ser Trp Glu Met Ala Lys Glu Cys Phe Thr Thr Asn 100 105 110 Asp Lys Phe Leu Ala Ser Arg Pro Thr Ser Ala Gly Gly Lys Tyr Leu 115 120 125 Thr Tyr Asp Phe Ala Met Phe Gly Phe Ser Phe Tyr Gly Pro Tyr Trp 130 135 140 Arg Glu Ile Arg Lys Ile Ser Thr Leu Glu Leu Leu Ser His Arg Arg 145 150 155 160 Val Glu Leu Leu Lys His Val Pro Tyr Thr Glu Ile Gly Gly Ser Ile 165 170 175 Lys Gln Leu Tyr Lys Leu Trp Met Glu Thr Gln Asn Gln Asn Lys Gln 180 185 190 Arg Asp Asp His Gln Val Lys Val Asp Met Ser Gln Val Phe Gly Tyr 195 200 205 Leu Thr Leu Asn Thr Val Leu Lys Leu Val Val Gly Lys Gly Leu Phe 210 215 220 Asn Asn Asn Asp Met Asn His Glu Gln Glu Glu Gly Arg Lys Leu His 225 230 235 240 Glu Thr Val Leu Glu Phe Phe Lys Leu Ala Gly Val Ser Val Ala Ser 245 250 255 Asp Ala Leu Pro Phe Leu Gly Trp Leu Asp Val Asp Gly Gln Lys Arg 260 265 270 Ser Met Lys Arg Ile Ala Lys Glu Met Asp Leu Ile Ala Glu Arg Trp 275 280 285 Leu Gln Glu His Arg Gln Lys Arg Leu Thr Ser Asn Asn Lys Ala Ser 290 295 300 Ser Gly His Asp Asp Phe Met Ser Val Leu Leu Ser Ile Leu Asp Asp 305 310 315 320 Asp Ser Asn Phe Phe Asn Tyr Asn Arg Asp Thr Val Ile Lys Ala Thr 325 330 335 Ser Leu Asn Leu Ile Leu Ala Ala Ser Asp Thr Thr Ser Val Ser Leu 340 345 350 Thr Trp Val Leu Ser Leu Leu Val Thr Asn Pro Gly Ala Leu Lys Lys 355 360 365 Val Gln Asp Glu Leu Asp Thr Lys Val Gly Arg Asn Arg His Val Glu 370 375 380 Glu Arg Asp Ile Glu Lys Leu Val Tyr Leu Gln Ala Thr Val Lys Glu 385 390 395 400 Thr Leu Arg Met Tyr Pro Ala Gly Pro Leu Ser Val Pro His Glu Ala 405 410 415 Thr Gln Asp Cys Thr Val Gly Gly Tyr Gln Val Thr Ala Gly Thr Arg 420 425 430 Leu Val Val Asn Val Trp Lys Leu Gln Arg Asp Pro Arg Val Trp Pro 435 440 445 Asn Pro Ser Glu Phe Lys Pro Glu Arg Phe Leu Pro Asp Gly Cys Glu 450 455 460 Val Gly Cys Gly Glu Ala Ala Asn Met Asp Phe Arg Gly Gln His Phe 465 470 475 480 Glu Tyr Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Ile Asp 485 490 495 Phe Ala Ile Gln Ile Ile His Met Thr Leu Ala Cys Leu Leu His Ala 500 505 510 Phe Glu Phe Gln Val Pro Ser Ser Leu Asp Lys His Leu Val Pro Ala 515 520 525 Val Ile Asp Met Ser Glu Gly Ser Gly Leu Thr Met Pro Lys Val Thr 530 535 540 Pro Leu Glu Val Leu Leu Asn Pro Arg Leu Pro Leu Pro Leu Tyr Glu 545 550 555 560 Leu <210> 237 <211> 522 <212> PRT <213> Cissampleos mucronata <400> 237 Met Glu Ala Met Gly Glu Tyr Phe Gln Leu Ile Ser Trp Thr Val Phe 1 5 10 15 Ser Ala Leu Ile Thr Ile Tyr Phe Ile Phe Ser Arg Lys Thr Asn Gly 20 25 30 Lys Lys Lys Ala Pro Glu Pro Gly Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu His Leu Phe Gly Ala His Asp Leu Leu Tyr Arg Lys Leu Gly Ala 50 55 60 Met Ala Asp Lys Leu Gly Pro Val Phe Met Ile Arg Phe Gly Met His 65 70 75 80 Arg Ala Val Val Val Ser Asn Tyr Glu Val Ala Lys Glu Cys Phe Thr 85 90 95 Val Asn Asp Lys Ile Leu Ala Ser Arg Pro Arg Ile Ile Ala Ser Lys 100 105 110 Leu Met Gly Tyr Asn His Ala Ala Val Gly Leu Ser Pro Tyr Gly Pro 115 120 125 Tyr Trp Arg Glu Ala Arg Lys Ile Ala Thr Leu Glu Leu Leu Ser Asn 130 135 140 His Arg Leu Gln Leu Leu Lys His Ile Arg Ile Ser Glu Ile Asp Met 145 150 155 160 Trp Ile Lys Glu Leu Lys Glu Phe Cys Met Lys Ser Ser Ser Asp Gly 165 170 175 Pro Val Leu Val Gln Leu Asp Gly Trp Phe Asn Ala Leu Thr Leu Asn 180 185 190 Ile Met Val Arg Asn Ile Ala Gly Lys Arg Tyr Cys Gly Gly Ala Glu 195 200 205 Ala Phe Glu Asp Glu Glu Ser Gln Arg Trp Lys Lys Val Ser Arg Glu 210 215 220 Leu Met Phe Leu Phe Gly Val Phe Met Val Pro Asp Ala Phe Pro Val 225 230 235 240 Leu Glu Gly Ile Asp Val Gln Gly Phe Glu Arg Ala Met Lys Arg Val 245 250 255 Gly Lys Glu Met Asp Phe Phe Leu Ser Arg Trp Leu Glu Glu His Arg 260 265 270 Arg Lys Lys Ile Glu Leu Ser Glu Lys Gly Asn Glu Val Asn Asp Gln 275 280 285 Gly Ala Gln Asp Phe Ile Asp Val Met Leu Asn Thr Ile Lys Asp Ala 290 295 300 Lys Ile Phe Glu His Asp Ala Asp Thr Met Ile Lys Ala Thr Cys Met 305 310 315 320 Ala Leu Val Val Ala Gly Asn Asp Thr Thr Met Ile Thr Leu Ser Trp 325 330 335 Ala Val Ser Leu Leu Leu Asn Asn Arg Gly Val Leu Glu Lys Ala Gln 340 345 350 Glu Glu Leu Ser Asn Gln Ile Gly Lys Asn Arg His Val Glu Glu Gly 355 360 365 Asp Ile Glu Ser Leu Pro Tyr Leu His Ala Ile Val Lys Glu Thr Phe 370 375 380 Arg Leu Tyr Pro Ala Ala Pro Leu Ser Leu Pro His Glu Ala Met Glu 385 390 395 400 Asp Cys Thr Ile Ala Gly Phe His Val Pro Ala Gly Thr Arg Leu Ile 405 410 415 Thr Asn Ile Trp Lys Leu His Lys Asp Pro Asn Ile Trp Pro Asp Pro 420 425 430 Leu Glu Phe Lys Pro Glu Arg Phe Leu Thr Thr His Ala His Val Asp 435 440 445 Phe Lys Gly Gln His Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg 450 455 460 Met Cys Pro Gly Ser Ser Leu Ala Ile Ser Val Leu His Leu Thr Leu 465 470 475 480 Ala Arg Leu Leu His Glu Phe Glu Leu Lys Thr Pro Asp Asp Ala Pro 485 490 495 Val Asp Met Thr Glu Gly Pro Gly Ile Thr Leu Met Arg Ala Asn Pro 500 505 510 Leu His Val Leu Ile Asn Pro Arg His Asp 515 520 <210> 238 <211> 534 <212> PRT <213> Cissampleos mucronata <400> 238 Met Ser Ser Pro Ile Gln Gln Leu Leu Leu Ser Leu Thr Ser Ile Asn 1 5 10 15 Gly Gly Thr Ile Ile Ser Ala Val Ile Ile Ser Leu Leu Leu Ile Leu 20 25 30 Thr Leu His Arg Leu Leu Lys Pro Ile Asn Ser Asn Lys Thr Lys His 35 40 45 Pro Pro Arg Ala Ser Gly Ala Trp Pro Val Met Gly His Leu His Lys 50 55 60 Leu Arg Gly Pro Asp Leu Pro His Arg Thr Leu Ser Ser Met Ala Asp 65 70 75 80 Gln Tyr Gly Pro Ile Phe Thr Ile Asn Leu Gly Pro Thr Thr Ala Leu 85 90 95 Val Ile Ser Asp Pro Ala Val Ala Lys Glu Cys Phe Thr Thr Asn Asp 100 105 110 Leu Thr Phe Ser Ser Arg Pro Thr Thr Leu Ala Thr Thr Leu Met Cys 115 120 125 Tyr Asn Asn Thr Met Phe Gly Phe Ala Pro Tyr Gly Pro Tyr Trp Arg 130 135 140 Glu Val Arg Lys Val Val Met Ala Gln Leu Leu Ser Thr Arg Arg Leu 145 150 155 160 Glu Ser Leu Lys Asn Ile Trp Ala Ser Glu Ile Asp Arg Trp Val Lys 165 170 175 Gly Leu Ala Gln Lys Ala Gln Ser Gly Ser His Gly Val Val Glu Val 180 185 190 Glu Met Gly Asp Trp Leu Ala Arg Leu Thr Met Asn Ile Gly Leu Arg 195 200 205 Leu Val Val Gly Lys Ser Cys Gly Glu Met Gly Ser Glu Glu Ser Glu 210 215 220 Arg Cys Leu Gly Ala Met Arg Glu Tyr Pro Glu Leu Leu Gly Arg Phe 225 230 235 240 Ala Leu Glu Asp Ala Leu Pro Trp Leu Gly Arg Phe Asp Leu Gln Gly 245 250 255 His Gln Arg Glu Met Arg Arg Ala Ala Arg Val Leu Asp Glu Ile Leu 260 265 270 Asp Gly Trp Leu Asp Glu His Lys Arg Asn Arg Ser Gly Leu Ser Gly 275 280 285 Gly Asp Asp His Arg Asp Gln Asp Phe Met Asp Val Met Leu Ser Val 290 295 300 Leu Glu Gln Asp Gln Asp Ser Ser Leu Ser Asp His Asp Ala Asp Met 305 310 315 320 Ile Asn Lys Ser Thr Cys Leu Asn Leu Ile Leu Gly Met Ala Asp Thr 325 330 335 Thr Met Val Ala Leu Thr Trp Ala Ile Ser Leu Leu Leu Asn Asn Lys 340 345 350 Thr Ser Leu Lys Lys Ala Gln Glu Glu Leu Gln Ala Gln Val Gly Asn 355 360 365 Glu Arg His Val Asp Glu Ser Asp Val Lys Asn Leu Val Tyr Leu Gln 370 375 380 Ser Ile Ile Lys Glu Val Leu Arg Leu Tyr Pro Pro Glu Pro Leu Ser 385 390 395 400 Gly Pro Arg Glu Ala Leu Glu Asp Cys Glu Val Ala Gly Tyr His Val 405 410 415 Pro Arg Gly Thr Arg Leu Ile Ala Asn Leu Trp Lys Ile His Arg Asp 420 425 430 Ser Ser Thr Trp Gln Asp Ser Met Glu Phe Arg Pro Glu Arg Phe Leu 435 440 445 Thr Ala His Glu His Val Asp Val Trp Gly Gln His Phe Glu Phe Met 450 455 460 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Val Ser Phe Glu Val 465 470 475 480 Arg Val Leu Pro Leu Ile Leu Ala Arg Leu Ile His Ala Phe Glu Met 485 490 495 Thr Thr Arg Gly Asp Val Pro Val Asp Met Ser Glu Arg Pro Gly Leu 500 505 510 Val Ile Ser Lys Ala Ser Pro Leu Glu Val Met Ile Ser Pro Arg Leu 515 520 525 Pro Leu His Leu Phe Glu 530 <210> 239 <211> 536 <212> PRT <213> Cissampleos mucronata <400> 239 Met Glu Phe His Leu Leu Leu Gln Ala Val Ala Ala Thr Leu Val Thr 1 5 10 15 Phe Phe Leu Tyr Glu Leu Trp Ala Leu Arg Lys Lys Phe Ser Arg Leu 20 25 30 Thr Lys Thr Pro Ser Ser Lys Ala Leu Leu Lys Ala Lys Cys Ala Pro 35 40 45 Glu Pro Pro Gly Ala Trp Pro Ile Ile Gly His Leu Pro Leu Leu Val 50 55 60 Ser Ala Lys Gln Pro His Arg Ala Phe Ala Ala Leu Ala Glu Lys Tyr 65 70 75 80 Gly Pro Ala Phe Met Leu Arg Met Gly Met Ser Pro Met Leu Ile Val 85 90 95 Ser Thr Lys Glu Val Ala Lys Glu Cys Tyr Thr Ala Asn Asp His Val 100 105 110 Phe Ala Thr Arg Pro Val Thr Thr Ala Gly Lys Leu Met Ala Tyr Asp 115 120 125 His Ser Val Met Gly Phe Thr Pro Phe Gly Thr Tyr Trp Arg Glu Ile 130 135 140 Arg Lys Ile Ala Thr Val Glu Leu Phe Ser Ala Arg Arg Ile Gly Met 145 150 155 160 Leu Lys Pro Val Arg Gln Ser Glu Val Ser Leu Trp Met Lys Gly Leu 165 170 175 His Glu Lys Trp Val His Asn Gly Asn Ser Ser Val Ser Val Glu Leu 180 185 190 Lys Ser Gln Leu Glu Glu Leu Thr Phe Asn Leu Leu Thr Gln Met Val 195 200 205 Ala Gly Lys Arg Tyr Tyr Gly Ser Asn Val Ala Lys Val Asp Glu Lys 210 215 220 Met Ala Gly Leu Phe Arg His Ala Val Gln Gln Phe Asn Tyr His Leu 225 230 235 240 Gly Asn Ser Glu Met Tyr Asp Ala Leu Pro Phe Met Ala Trp Leu Asp 245 250 255 Phe Lys Gly Asp Ala Lys Ala Met Lys Lys Thr Gln Lys Asp Leu Asp 260 265 270 Phe Ile Met Gln Thr Trp Leu Asp Glu His Arg Leu Lys Ala Asp Gln 275 280 285 Met Arg Gly Asp Ala Ile Asn Asn Thr Arg Asp Phe Leu Asp Val Leu 290 295 300 Val Met Met Glu Lys Thr Gly Gln Phe Ser Ser Ala Ile Lys Asp Arg 305 310 315 320 Asp Thr Thr Ile Lys Ala Leu Ala Leu Thr Gln Leu Val Ala Gly Val 325 330 335 Asp Ser Met Ala Asn Thr Met Val Trp Val Leu Ala Leu Leu Met Asn 340 345 350 Asn Pro Glu Met Leu Ala Lys Ala Gln Asp Glu Leu Asp Asn Asn Val 355 360 365 Gly Lys Asp Arg Leu Val Glu Glu Ser Asp Ile Pro Asn Leu Lys Tyr 370 375 380 Leu Gln Ala Leu Leu Lys Glu Thr Leu Arg Met Tyr Pro Val Gly Pro 385 390 395 400 Leu Leu Val Pro His Glu Ala Thr Glu Asp Cys His Val Ala Gly Tyr 405 410 415 Phe Val Pro Arg Gly Thr Gly Leu Phe Ile Asn Ala Trp Thr Ile Gln 420 425 430 Arg Asp Pro Asn Val Trp Ala Glu Pro Asn Cys Phe Asn Pro Glu Arg 435 440 445 Phe Leu Thr Thr His Ala Glu Met Asp Val Lys Gly Gln Asn His Glu 450 455 460 Leu Leu Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Val Gly Leu 465 470 475 480 Ala Leu Gln Val Met His Leu Thr Leu Ala Arg Ile Leu Gln Ala Phe 485 490 495 Glu Leu Lys Thr Gln Ser Gly Val Gly Ile Asp Leu Glu Glu Cys Ser 500 505 510 Gly Ile Leu Leu Ser Met Met His Pro Leu Gln Val Met Met Ala Pro 515 520 525 Arg Leu Pro Ser Glu Leu Tyr Asp 530 535 <210> 240 <211> 524 <212> PRT <213> Eschscholzia californica <400> 240 Met Asp Ser Leu Ile Leu Thr Asn Trp Phe Pro Ile Ser Ile Ala Ser 1 5 10 15 Val Leu Thr Leu Val Phe Leu Tyr Lys Val Leu Leu Ser Ser Arg Thr 20 25 30 Leu Lys Asp Lys Lys Ile Lys Thr Ser Pro Met Ala Asn Gly Ala Trp 35 40 45 Pro Ile Leu Gly His Leu His Leu Phe Gly Ser Gly Glu Leu Pro His 50 55 60 Lys Met Leu Ala Thr Met Ala Asp Lys Tyr Gly Ser Ala Phe Arg Met 65 70 75 80 Lys Phe Gly Lys His Thr Thr Leu Val Val Ser Asp Thr Arg Ile Val 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ser Asn Arg Pro Ser 100 105 110 Thr Lys Ala Phe Gln Leu Met Thr Tyr Asp Asn Glu Ser Val Ala Phe 115 120 125 Thr Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr Leu 130 135 140 Lys Leu Leu Ser Asn His Arg Leu Gln Ala Ile Lys Asp Val Arg Ala 145 150 155 160 Ser Glu Val Asn Val Cys Phe Lys Ser Leu Tyr Asp Gln Cys Lys Asn 165 170 175 Pro Ser Gly Ala Pro Ile Leu Ile Asp Met Lys Lys Trp Phe Glu Glu 180 185 190 Val Ser Asn Asn Val Val Met Arg Val Ile Val Gly Arg Gln Asn Phe 195 200 205 Gly Ser Lys Ile Val Gln Gly Glu Glu Glu Ala Ile His Tyr Lys Lys 210 215 220 Val Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Met Phe Ser Asp 225 230 235 240 Phe Ala Pro Leu Leu Gly Phe Leu Asp Ile Phe Gln Gly Asn Leu Ser 245 250 255 Ala Met Lys Gln Asn Ala Lys Lys Val Asp Ala Ile Leu Glu Asn Trp 260 265 270 Leu Glu Glu His Arg Lys Lys Lys Asn Ser Val Ala Glu Ser Glu Gln 275 280 285 Asp Phe Met Asp Val Met Leu Ser Ile Ala Asn Glu Ser Lys Leu Ser 290 295 300 Gly His Asp Ala Asp Thr Val Ile Lys Ala Thr Cys Leu Ala Met Ile 305 310 315 320 Met Gly Gly Thr Asp Thr Thr Ala Val Ser Leu Thr Trp Ile Ile Ser 325 330 335 Leu Leu Met Asn Asn Arg His Ala Leu Lys Lys Ala Arg Glu Glu Leu 340 345 350 Asp Ala Leu Val Gly Lys Asp Arg Gln Val Glu Asp Ser Asp Leu Lys 355 360 365 Asn Leu Val Tyr Met Asn Ala Ile Val Lys Glu Thr Met Arg Met Tyr 370 375 380 Pro Leu Gly Thr Leu Leu Glu Arg Asp Thr Lys Glu Asp Cys Glu Ile 385 390 395 400 Gly Gly Phe His Val Lys Gly Gly Thr Arg Leu Leu Val Asn Val Trp 405 410 415 Lys Leu Gln Arg Asp Pro Asn Val Trp Val Asp Pro Thr Glu Phe Arg 420 425 430 Pro Glu Arg Phe Leu Thr Glu Asn Ala Asp Ile Asp Val Gly Gly Gln 435 440 445 His Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly 450 455 460 Val Ser Phe Ala Leu Gln Phe Met His Leu Val Leu Ala Arg Leu Ile 465 470 475 480 His Gly Tyr Asp Leu Asn Thr Leu Asn Glu Glu Asn Val Asp Leu Thr 485 490 495 Glu Ser Pro Glu Gly His Val Asn His Lys Ala Ser Pro Leu Asp Leu 500 505 510 Ile Leu Thr Pro Arg Leu His Tyr Lys Leu Tyr Glu 515 520 <210> 241 <211> 532 <212> PRT <213> Eschscholzia californica <400> 241 Met Asp Thr Ala Ser Ser Ile Ser Leu Leu Leu Pro Trp Ala Val Val 1 5 10 15 Val Pro Ser Ile Ala Thr Leu Leu Ala Leu Leu Leu Phe Leu Phe Ile 20 25 30 Thr Ser Pro Lys Thr Pro Lys Arg Lys Thr Ser Ser Lys Pro Pro Pro 35 40 45 Thr Val Leu Pro Gly Ala Trp Pro Ile Leu Gly His Leu His Leu Phe 50 55 60 Lys Glu Gly Glu Ser Pro His His Met Leu Lys Asn Leu Ala Asp Lys 65 70 75 80 Tyr Gly Pro Ala Phe Val Met Lys Phe Gly Gln His Arg Ser Leu Val 85 90 95 Val Ser Asn Thr Lys Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Thr 100 105 110 Leu Phe Ser Asn Arg Pro Ser Thr Thr Ala Phe Asp Leu Met Thr Tyr 115 120 125 Ala His Asp Ser Val Ala Phe Thr Pro Tyr Ser Pro Tyr Trp Arg Glu 130 135 140 Leu Arg Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn Asn Arg Leu Lys 145 150 155 160 Ala Ile Lys Lys Leu Arg Gly Glu Glu Val Asp Val Cys Phe Arg Gly 165 170 175 Leu Tyr Gly Leu Trp Lys Asn Lys Thr Lys Asn Gly Ala Pro Val Leu 180 185 190 Val Asp Met Lys Lys Trp Phe Glu Glu Val Ala Asn Asn Val Val Ile 195 200 205 Arg Val Ile Val Gly Lys Leu Ser Phe Gly Thr Lys Ile Val Asp Gly 210 215 220 Glu Glu Glu Ala Val Glu Tyr Lys Thr Val Met Asp Glu Leu Leu Arg 225 230 235 240 Leu Ala Ser Leu Ser Leu Leu Ser Asp Met Ala Pro Ile Leu Gly Trp 245 250 255 Leu Asp Phe Phe Gln Gly Ser Val Arg Lys Met Lys Gln Thr Gly Lys 260 265 270 Arg Leu Asp Val Leu Leu Glu Lys Trp Leu Gly Glu His Arg Glu Lys 275 280 285 Lys Asn Leu Val Gly Glu Asp Glu Gln Asp Phe Met Asp Val Met Leu 290 295 300 Ser Ile Val Glu Glu Ser Lys Leu Ser Gly His Glu Ala Asp Ala Val 305 310 315 320 Ile Lys Ala Thr Cys Leu Ala Met Ile Met Gly Gly Thr Asp Thr Thr 325 330 335 Ala Val Thr Leu Thr Trp Ile Ile Ser Leu Leu Met Asn Asn Arg His 340 345 350 Ala Leu Glu Lys Ala Arg Glu Glu Leu Glu Thr His Val Gly Lys Asp 355 360 365 Arg Gln Val Glu Asp Thr Asp Leu Gln Asn Leu Val Tyr Leu Ser Ala 370 375 380 Ile Val Lys Glu Thr Met Arg Leu Tyr Pro Leu Gly Thr Leu Leu Glu 385 390 395 400 Arg Glu Thr Lys Glu Asp Cys Glu Val Gly Gly Phe His Ile Gln Gly 405 410 415 Gly Thr Arg Leu Leu Val Asn Ile Trp Met Val Gln Arg Asp Pro Thr 420 425 430 Val Trp Asn Asp Pro Ser Ala Phe Lys Pro Glu Arg Phe Leu Thr Asp 435 440 445 Lys Ser Glu Ile Asp Val Gly Gly Gln His Phe Glu Leu Ile Pro Phe 450 455 460 Gly Ala Gly Arg Arg Val Cys Pro Ala Val Ser Phe Ala Leu Gln Phe 465 470 475 480 Leu His Leu Val Leu Ala Arg Leu Ile His Gly Tyr Asp Leu Gly Thr 485 490 495 Pro Ser Asn Val Glu Val Asp Leu Thr Glu Ser Leu Glu Gly His Val 500 505 510 Asn His Lys Ala Ser Pro Leu Glu Leu Leu Leu Thr Pro Arg Leu Asn 515 520 525 Pro Lys Leu Tyr 530 <210> 242 <211> 548 <212> PRT <213> Eschscholzia californica <400> 242 Met Glu Ser Glu Ile Gln Tyr Gln Thr Ala Thr Met Ala Ser Ile Leu 1 5 10 15 Ser Thr Asn Leu Ile Leu Ser Ser Ile Phe Thr Ala Phe Leu Leu Tyr 20 25 30 Phe Leu Leu Lys Met Ser Pro Phe Arg Ser Ser Lys Asn Lys Ser Arg 35 40 45 Lys Gln Ala Pro Lys Pro Thr Gly Ser Trp Pro Ile Ile Gly His Leu 50 55 60 Leu His Leu Gln Gly Pro Asn Leu Pro His Ile Asn Leu Ala Ala Leu 65 70 75 80 Ala Asp Lys Tyr Gly Ser Val Phe Ser Phe Arg Ile Gly Leu Arg Pro 85 90 95 Ala Leu Val Val Ser Ser Trp Glu Ile Ala Lys Glu Cys Leu Thr Thr 100 105 110 Asn Asp Arg Val Phe Val Ser Arg Pro Pro Leu Ile Ala Met Lys His 115 120 125 Met Gly Tyr Asp His Ala Leu Phe Gly Phe Ser Pro Tyr Gly Pro Tyr 130 135 140 Trp Arg Glu Leu Arg Lys Leu Val Asn Gln Glu Leu Leu Ser Asn Thr 145 150 155 160 Arg Ile Glu Leu Ile Lys His Val Trp Asp Thr Glu Ile Asn Thr Phe 165 170 175 Ile Asn Asp Leu Tyr Glu Val Trp Ala Met Lys Ser Asn Glu Gly Gly 180 185 190 Gly Asp Val Val Val Glu Met Lys Gln Cys Leu Tyr Asp Phe Thr Leu 195 200 205 Asn Leu Thr Leu Lys Ile Leu Thr Gly Lys Arg Tyr Phe Gly Gly Gly 210 215 220 Gly Ser Glu Glu Leu Arg Glu Glu Ala Gly Arg Cys Gln Lys Ala Val 225 230 235 240 Arg Asn Phe Leu Arg Leu Val Gly Thr Phe Thr Ala Gln Asp Val Ile 245 250 255 Pro Phe Leu Glu Gly Trp Leu Asp Leu Gly Gly His Glu Lys Glu Met 260 265 270 Lys Ile Thr Gly Lys Glu Leu Asp Ser Leu Leu Gln Lys Trp Leu Glu 275 280 285 Glu His Lys Val Lys Lys Ser Ser Ala Gly Glu Glu Gly Gln Glu Asn 290 295 300 Arg Gly Gly Asp Glu Glu Asp Phe Met Gly Val Met Leu Thr Lys Leu 305 310 315 320 Arg Asp His Glu Lys Leu Leu Ser Tyr Tyr Asp Ala Asp Thr Ile Asn 325 330 335 Lys Ala Thr Cys Leu Thr Val Ile Leu Gly Gly Ser Asp Thr Thr Met 340 345 350 Met Thr Leu Val Trp Ala Leu Ala Leu Leu Val Asn His Pro His Val 355 360 365 Met Lys Lys Leu Gln Asp Glu Leu Asp Thr His Val Ser Lys Glu Arg 370 375 380 Gln Val Glu Glu Ser Asp Ile Lys Asn Leu Ala Tyr Leu Gln Ser Val 385 390 395 400 Met Lys Glu Thr Met Arg Arg Tyr Pro Gly Thr Pro Leu Tyr Val Arg 405 410 415 Glu Ser Ile Glu Asp Cys Thr Ile Ala Gly Tyr Asp Val Pro Thr Gly 420 425 430 Thr Arg Leu Val Val Asn Ala Trp Lys Ile Gln His Asp Pro Gln Val 435 440 445 Trp Ser Asn Pro Phe Glu Phe Asn Pro Glu Arg Phe Leu Thr Thr His 450 455 460 Lys Asp Ile Asp Val Arg Gly Gln Asn Phe Glu Leu Ile Pro Phe Gly 465 470 475 480 Ala Gly Arg Arg Met Cys Pro Gly Ala Ser Leu Gly Leu Gln Val Val 485 490 495 His Leu Thr Leu Ala Arg Leu Val His Gly Phe Glu Phe Lys Thr Pro 500 505 510 Asp Gly Glu Pro Met Asp Met Thr Glu Ser Ile Gly Leu Thr Asn Leu 515 520 525 Lys Ala Thr Pro Leu Glu Ile Met Leu Thr Pro Arg Leu Ser Ser Lys 530 535 540 Leu Tyr Val Cys 545 <210> 243 <211> 554 <212> PRT <213> Eschscholzia californica <400> 243 Met Gly Leu Ser Phe Asp Leu Ser Ser His Asn Phe Leu Gln Phe Ser 1 5 10 15 Asn Pro Thr Ile Leu Ile Gly Leu Phe Gly Leu Leu Val Tyr Leu Leu 20 25 30 Leu Val Asn Leu Arg Lys Arg Ile Ser Arg Lys Asn Glu Ala Pro Glu 35 40 45 Val Glu Gly Gly Trp Pro Ile Ile Gly His Leu His His Phe Met Gly 50 55 60 Gly Lys Asn Lys Leu Leu His Val Ala Phe Gly Ala Trp Ala Asp Lys 65 70 75 80 Tyr Gly Pro Val Tyr Thr Leu Arg Met Gly Leu Asn Lys Val Leu Val 85 90 95 Val Asn Ser Ala Glu Val Ala Lys Glu Cys Ser Thr Thr Asn Asp Met 100 105 110 Leu Phe Met Ala Arg Pro Tyr Arg Ile Ala Ser Glu Ile Met Gly Tyr 115 120 125 Gly Tyr Ala Met Phe Pro Ile Ala Pro Tyr Gly Pro Phe Tyr Leu Lys 130 135 140 Met Arg Lys Met Val Thr Gln Glu Leu Leu Ser Asn Ser Arg Val Asp 145 150 155 160 Ser Leu Lys His Val Trp Gly Ser Glu Ile Lys Asn Ala Ile Gln Glu 165 170 175 Leu His Lys Lys Val Leu Ser Thr Lys Gly Gly Gly Pro Ile Ser Met 180 185 190 Asp Met Lys Gly Trp Val Ser Asn Leu Thr Phe Arg Thr Ala Met Lys 195 200 205 Val Ile Cys Gly Gly Val Gly Val Asp Gly Gly Gly Gly Gly Gly Gly 210 215 220 Ala Thr Ser Pro Ala Thr Ser Ile Gly Glu His Asn Tyr Asp Glu Val 225 230 235 240 Gly Ser Phe Gln Lys Ala Leu Lys Glu Phe Phe Val Leu Leu Gly Glu 245 250 255 Ile Arg Ile Ser Asp Val Ile Pro Phe Leu Gly Trp Leu Asp Met Arg 260 265 270 Thr Gly Tyr Val Glu Lys Met Lys Asp Asn Gly Lys Phe Leu Asp Asn 275 280 285 Leu Met Glu Glu Met Leu Glu Glu His Lys Arg Lys Arg Arg Ser Leu 290 295 300 Thr Glu Glu Glu Glu Lys Asp Gly Gly Tyr Val Glu Gln Asp Phe Met 305 310 315 320 Asp Val Met Ile Ser Lys Leu Asn Asp Pro Lys Leu Leu Ser Tyr Tyr 325 330 335 Asp Ala Asp Thr Ile Asn Lys Ser Thr Cys Leu Thr Leu Ile Leu Ser 340 345 350 Gly Ser Glu Thr Thr Met Val Ser Ile Val Trp Ala Leu Ala Leu Leu 355 360 365 Val Ser His Pro His Val Leu Lys Lys Ala Gln Asp Glu Leu Asp Thr 370 375 380 Leu Val Gly Arg Glu Arg Gln Val Asp Glu Ser Asp Ile Lys Asp Leu 385 390 395 400 Val Tyr Leu Gln Ala Val Val Lys Glu Ala Leu Arg Leu Phe Pro Pro 405 410 415 Ala Pro Leu Ser Thr Pro Arg Val Ala Thr Glu Asp Cys Val Val Ser 420 425 430 Gly Tyr His Val Pro Ala Gly Thr Gln Leu Phe Val Asn Thr Trp Lys 435 440 445 Ile Gln Arg Asn Pro Glu Val Trp Pro Glu Pro Ser Glu Phe Arg Pro 450 455 460 Glu Arg Phe Phe Thr Thr His Lys Asp Phe Asp Val Arg Gly Leu His 465 470 475 480 Tyr Asp Leu His Pro Phe Gly Ser Gly Arg Arg Ala Cys Pro Gly Ala 485 490 495 Gly Phe Ala Leu Gln Val Val His Leu Thr Leu Ala Ser Leu Leu His 500 505 510 Gly Phe Glu Ile Lys Asn Pro Ser Asp Glu Pro Ile Asp Met Thr Glu 515 520 525 Ser Pro Gly Val Thr Asn Leu Lys Ala Thr Pro Leu Lys Val Leu Leu 530 535 540 Thr Pro Arg Leu Phe Ser Lys Glu Val Tyr 545 550 <210> 244 <211> 561 <212> PRT <213> Eschscholzia californica <400> 244 Met Glu Lys Pro Ile Leu Leu Gln Leu Gln Pro Gly Ile Leu Gly Leu 1 5 10 15 Leu Ala Leu Met Cys Phe Leu Tyr Tyr Val Ile Lys Val Ser Leu Ser 20 25 30 Thr Arg Asn Cys Asn Gln Leu Val Arg His Pro Pro Glu Ala Ala Gly 35 40 45 Ser Trp Pro Ile Val Gly His Leu Pro Gln Leu Val Gly Ser Gly Lys 50 55 60 Pro Leu Phe Arg Val Leu Gly Asp Met Ala Asp Lys Phe Gly Pro Ile 65 70 75 80 Phe Met Val Arg Phe Gly Val His Pro Thr Leu Val Val Ser Ser Trp 85 90 95 Glu Met Ala Lys Glu Cys Phe Thr Ser Asn Asp Lys Phe Leu Ala Ser 100 105 110 Arg Pro Pro Ser Ala Ala Ser Ile Tyr Met Ala Tyr Asp His Ala Met 115 120 125 Leu Gly Phe Ser Ser Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys Ile 130 135 140 Ser Thr Leu His Leu Leu Ser His Arg Arg Leu Glu Leu Leu Lys His 145 150 155 160 Val Pro His Leu Glu Ile His Asn Phe Ile Lys Gly Leu Tyr Gly Ile 165 170 175 Trp Lys Asp His Gln Lys Gln Gln Gln Gln Pro Thr Ala Arg Asp Asp 180 185 190 Gln Asp Ser Val Met Leu Glu Met Ser Gln Leu Phe Gly Tyr Leu Thr 195 200 205 Leu Asn Ile Val Leu Ser Leu Val Val Gly Lys Arg Val Cys Asn Tyr 210 215 220 His Ala Asp Gly His Leu Asp Asp Gly Glu Glu Ala Gly Gln Gly Gln 225 230 235 240 Lys Leu His Gln Thr Ile Thr Asp Phe Phe Lys Leu Ser Gly Val Ser 245 250 255 Val Ala Ser Asp Ala Leu Pro Phe Leu Gly Leu Phe Asp Leu Asp Gly 260 265 270 Gln Lys Lys Ile Met Lys Arg Val Ala Lys Glu Met Asp Phe Val Ala 275 280 285 Glu Arg Trp Leu Gln Asp Lys Lys Ser Ser Leu Leu Leu Ser Ser Lys 290 295 300 Ser Asn Asn Lys Gln Asn Glu Ala Gly Glu Gly Asp Val Asp Asp Phe 305 310 315 320 Met Asp Val Leu Met Ser Thr Leu Pro Asp Asp Asp Asp Ser Phe Phe 325 330 335 Thr Lys Tyr Ser Arg Asp Thr Val Ile Lys Ala Asn Ser Leu Ser Met 340 345 350 Val Val Ala Gly Ser Asp Thr Thr Ser Val Ser Leu Thr Trp Ala Leu 355 360 365 Ser Leu Leu Leu Asn Asn Ile Gln Val Leu Arg Lys Ala Gln Asp Glu 370 375 380 Leu Asp Thr Lys Val Gly Arg Asp Arg His Val Glu Glu Lys Asp Ile 385 390 395 400 Asp Asn Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Met 405 410 415 Tyr Pro Ala Gly Pro Leu Ser Val Pro His Glu Ala Ile Glu Asp Cys 420 425 430 Asn Val Gly Gly Tyr His Ile Lys Thr Gly Thr Arg Leu Leu Val Asn 435 440 445 Ile Trp Lys Leu Gln Arg Asp Pro Arg Val Trp Ser Asn Pro Ser Glu 450 455 460 Phe Arg Pro Glu Arg Phe Leu Asp Asn Gln Ser Asn Gly Thr Leu Leu 465 470 475 480 Asp Phe Arg Gly Gln His Phe Glu Tyr Ile Pro Phe Gly Ser Gly Arg 485 490 495 Arg Met Cys Pro Gly Val Asn Leu Ala Thr Pro Ile Leu His Met Thr 500 505 510 Leu Ala Arg Leu Leu Gln Ser Phe Asp Leu Thr Thr Pro Ser Ser Ser 515 520 525 Pro Val Asp Met Thr Glu Gly Ser Gly Leu Thr Met Pro Lys Val Thr 530 535 540 Pro Leu Lys Val Leu Leu Thr Pro Arg Leu Pro Leu Pro Leu Tyr Asp 545 550 555 560 Tyr <210> 245 <211> 565 <212> PRT <213> Eschscholzia californica <400> 245 Met Asn Leu Ser Ser Lys Leu Met Gly Ser Phe Asp Leu Phe Ser Leu 1 5 10 15 Asn Phe Leu Gln Phe Ser Lys Pro Ala Ser Met Val Ile Ile Gly Ile 20 25 30 Cys Ser Phe Leu Val Tyr Phe Ser Leu Leu Asn Leu Val Arg Arg Val 35 40 45 Leu Ala Pro Ser Cys Thr Met Lys Asn Gly Lys Thr Glu Pro Pro Glu 50 55 60 Val Glu Asn Gly Trp Pro Ile Ile Gly His Leu His His Phe Met Gly 65 70 75 80 Lys Lys Asn Lys Leu Ile His Glu Ile Phe Gly Asp Met Ala Asp Lys 85 90 95 Tyr Gly Pro Thr Phe Thr Leu Arg Met Gly Leu Thr Lys Val Leu Val 100 105 110 Val Ser Ser Ala Glu Val Ala Lys Glu Cys Leu Thr Thr Asn Asp Leu 115 120 125 Val Phe Ile Gly Arg Pro Pro Arg Val Ala Asn Ser Leu Leu Gly Tyr 130 135 140 Ser Phe Ala Met Phe Pro Phe Ser Pro Tyr Gly Thr Tyr Tyr Ser Gln 145 150 155 160 Met Arg Lys Ile Val Thr His Glu Leu Leu Ser Thr Ser Arg Val Glu 165 170 175 Ser Leu Lys His Val Trp Asn Ser Glu Ile Asn Lys Ala Ile Gln Glu 180 185 190 Leu His His Lys Val Val Ser Val Gly Gly Gly Ser Pro Val Leu Ile 195 200 205 Glu Phe Lys Arg Trp Phe Ser Asp Leu Thr Leu Arg Thr Thr Val Lys 210 215 220 Leu Ile Cys Gly Lys Gln Tyr Phe Gly Thr Asp Gly Ala Thr Gln Ala 225 230 235 240 Ser Met Thr Ile Asn Gly Gly Gly Asp Asp Asp Glu Ala Gly Lys Phe 245 250 255 Gln Glu Ala Leu Arg Glu Phe Phe Cys Leu Leu Gly Lys Phe Arg Val 260 265 270 Ser Asp Val Ile Pro Phe Leu Gly Trp Leu Asp Phe Gly Thr Gly Tyr 275 280 285 Lys Glu Lys Lys Asn Arg Met Phe Ile Asp Ser Leu Met Glu Glu Trp 290 295 300 Leu Glu Glu His Lys Met Lys Arg Arg Leu Leu Asn Glu Ala Asp Lys 305 310 315 320 Lys Glu Ser Arg Ile Glu Gln Asp Phe Met Asp Val Met Ile Ser Lys 325 330 335 Leu Asp Asp Pro Asn Leu Leu Ser His Tyr Asp Ala Asp Thr Ile Asn 340 345 350 Lys Ala Thr Cys Leu Thr Leu Ile Leu Gly Gly Ser Asp Thr Thr Met 355 360 365 Val Ser Leu Val Trp Ala Leu Thr Leu Leu Met Asn His Pro His Val 370 375 380 Leu Lys Lys Val Gln Asp Glu Leu Asp Phe His Val Gly Arg Glu Arg 385 390 395 400 Gln Val Glu Glu Ser Asp Met Lys Asn Leu Val Tyr Leu His Ala Val 405 410 415 Met Lys Glu Ala Met Arg Leu Asn Pro Ala Gly Thr Leu Ser Ala Pro 420 425 430 Arg Met Ser Thr Lys Asp Cys Thr Val Ser Gly Tyr His Ile Pro Ala 435 440 445 Gly Thr His Leu Phe Met Asn Thr Trp Lys Ile Gln Arg Asp Pro Asn 450 455 460 Ala Trp Val Glu Pro Thr Glu Phe Arg Pro Glu Arg Phe Leu Thr Thr 465 470 475 480 His Lys Asp Phe Asp Leu Arg Gly Gln Asn Phe Glu Leu Leu Pro Phe 485 490 495 Gly Ser Gly Arg Arg Ser Cys Leu Gly Ala Asn Phe Ala Leu Gln Val 500 505 510 Leu Arg Gln Thr Leu Ala Arg Leu Leu His Gly Phe Asp Leu Lys Thr 515 520 525 Pro Ser Asp Glu Pro Val Asp Met Thr Gly Ser Ala Gly Leu Ile Asn 530 535 540 Met Lys Ala Thr Pro Leu Glu Val Leu Val Thr Pro Arg Leu Phe Ser 545 550 555 560 Ser Glu Leu Tyr Gly 565 <210> 246 <211> 550 <212> PRT <213> Eschscholzia californica <400> 246 Met Asn Leu Leu Ile Phe Phe Gln Phe Leu Leu Gln Phe Gln Val Leu 1 5 10 15 Val Gly Leu Ser Val Leu Leu Ala Phe Ser Tyr Tyr Leu Trp Val Ser 20 25 30 Lys Asn Pro Lys Ile Asn Lys Phe Lys Gly Lys Gly Ala Leu Leu Ala 35 40 45 Pro Gln Ala Ala Gly Ala Trp Pro Ile Val Gly His Leu Pro Gln Leu 50 55 60 Val Gly Pro Lys Pro Leu Phe Arg Ile Leu Gly Ala Met Ala Asp Asn 65 70 75 80 Tyr Gly Pro Ile Phe Met Leu Arg Phe Gly Val His Pro Thr Val Val 85 90 95 Val Ser Ser Trp Glu Met Thr Lys Glu Cys Phe Thr Thr Asn Asp Arg 100 105 110 His Leu Ala Ser Arg Pro Ser Asn Ala Ala Ser Gln Tyr Leu Ile Tyr 115 120 125 Glu Val Tyr Ala Leu Phe Gly Phe Ser Leu Tyr Gly Ser Ser Tyr Trp 130 135 140 Arg Asp Ala Arg Lys Ile Ala Thr Leu Glu Leu Leu Ser His Arg Arg 145 150 155 160 Leu Glu Leu Leu Lys His Val Pro Tyr Thr Glu Ile Asp Thr Cys Ile 165 170 175 Lys Gln Leu His Arg Leu Trp Thr Lys Asn Asn Lys Asn Gln Asn Asn 180 185 190 Pro Glu Leu Lys Val Glu Met Asn Gln Phe Phe Thr Asp Leu Thr Met 195 200 205 Asn Val Ile Leu Lys Leu Val Val Gly Lys Arg Phe Phe Asn Val Asp 210 215 220 Asp Ala Ala Asp His Glu Lys Glu Glu Ala Arg Lys Ile Gln Gly Thr 225 230 235 240 Ile Phe Glu Phe Phe Lys Leu Thr Glu Gly Ser Val Ser Ala Gly Ala 245 250 255 Leu Pro Leu Leu Asn Trp Leu Asp Leu Asn Gly Gln Lys Arg Ala Met 260 265 270 Lys Arg Thr Ala Lys Lys Met Asp Ser Ile Ala Glu Lys Leu Leu Asp 275 280 285 Glu His Arg Gln Lys Arg Leu Ser Lys Glu Gly Val Lys Gly Thr His 290 295 300 Asp His Asn Asp Phe Met Asp Val Leu Leu Ser Ile Leu Asp Ala Asp 305 310 315 320 Gln Gly Asp Tyr Ser His His Pro Phe Asn Tyr Ser Arg Asp His Val 325 330 335 Ile Lys Ala Thr Thr Leu Ser Met Ile Leu Ser Ser Met Ser Ile Ser 340 345 350 Val Ser Leu Ser Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His Val 355 360 365 Leu Lys Lys Ala Gln Asp Glu Leu Asp Met Asn Val Gly Lys Asp Arg 370 375 380 Gln Val Glu Glu Gly Asp Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile 385 390 395 400 Val Lys Glu Thr Phe Arg Met Tyr Pro Ala Asn Pro Leu Leu Leu Pro 405 410 415 His Glu Ala Ile Glu Asp Cys Lys Ile Gly Gly Phe Asn Val Pro Ala 420 425 430 Gly Thr Arg Val Val Val Asn Ala Trp Lys Leu Gln His Asp Pro Arg 435 440 445 Val Trp Ser Asn Pro Ser Glu Phe Lys Pro Glu Arg Phe Leu Asn Asp 450 455 460 Gln Ala Ala Lys Val Val Asp Val Arg Gly Gln Asn Phe Glu Tyr Leu 465 470 475 480 Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Ile Ser Phe Ser Leu 485 490 495 Gln Thr Ile His Met Ser Leu Ala Arg Leu Val Gln Ala Phe Glu Leu 500 505 510 Gly Thr Pro Ser Asn Glu Arg Ile Asp Met Thr Glu Gly Ser Gly Leu 515 520 525 Thr Met Pro Lys Thr Thr Pro Leu His Val Leu Leu Asn Pro Arg Leu 530 535 540 Pro Leu Pro Leu Tyr Glu 545 550 <210> 247 <211> 532 <212> PRT <213> Eschscholzia californica <400> 247 Met Glu Phe His Phe Pro Leu Met Glu Gln Phe Gln Pro Phe Phe Ile 1 5 10 15 Phe Ala Leu Leu Leu Ala Ser Phe Ile Phe Leu Tyr Lys Leu Phe Asn 20 25 30 Phe Gly Asn Arg Ile Thr Lys Asn Gly Lys Pro Thr Ala Pro Glu Ala 35 40 45 Ser Gly Gly Arg Leu Ile Met Gly His Leu His Leu Phe Asn Gly Thr 50 55 60 Glu Leu Thr His Arg Thr Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro 65 70 75 80 Ala Phe Asn Ile Arg Phe Gly Ser His Lys Thr Leu Val Val Ser Ser 85 90 95 Trp Lys Ile Ile Lys Glu Cys Phe Thr Thr Asn Asp Arg Phe Phe Ser 100 105 110 Asn Arg Pro Gly Ser Leu Ala Ile Lys Leu Met Phe Tyr Asp Ala Asp 115 120 125 Ser Val Gly Tyr Ala Pro Tyr Gly Ser Tyr Trp Arg Glu Leu Arg Lys 130 135 140 Ile Ser Thr Leu Lys Leu Leu Ser Asn His Arg Leu Glu Thr Leu Lys 145 150 155 160 His Leu Arg Thr Ser Glu Val Asp Ser Cys Phe Asn Gln Leu Met Asn 165 170 175 Ser Trp Ala Glu Asn Lys Asn Arg Gly Asp Ser Asp Phe Ala Pro Val 180 185 190 Arg Met Asp Asp Trp Phe Gly Asp Leu Thr Phe Asn Val Val Ala Arg 195 200 205 Ile Val Ala Gly Lys Lys Asn Phe Ala Gly Gly Ala Ala Arg Gly Asp 210 215 220 Ala Gly Ala Gln Arg Tyr Lys Ser Ala Met Asp Glu Ala Phe Arg Leu 225 230 235 240 Met Thr Ile Phe Ala Tyr Ser Asp Val Ile Pro Ser Leu Gly Trp Leu 245 250 255 Asp Lys Leu Arg Gly Leu Val Gly Asp Met Lys Arg Cys Gly Ser Glu 260 265 270 Ile Asp Ser Val Val Glu Ser Trp Val Asp Glu His Arg Leu Lys Arg 275 280 285 Arg Val Ser Lys Lys Gly Gly Glu Leu Asp Leu Glu Gln Asp Phe Ile 290 295 300 Asp Val Cys Leu Asp Ile Met Glu His Ser Ser Leu Pro Gly Asp Asp 305 310 315 320 Pro Glu Ile Val Ile Lys Ser Thr Cys Leu Asp Met Ile Leu Gly Gly 325 330 335 Ser Asp Thr Thr Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu 340 345 350 Asn His Pro Gln Val Leu Lys Arg Ala Lys Glu Glu Leu Asp Ser Gln 355 360 365 Val Gly Lys Glu Arg Gln Val Glu Asp Ser Asp Ile Pro Asn Leu Pro 370 375 380 Leu Ile Gln Ala Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly 385 390 395 400 Pro Leu Ile Glu Arg Arg Thr Met Glu Asp Cys Glu Val Ala Gly Phe 405 410 415 His Val Pro Ala Gly Thr Arg Leu Leu Val Asn Leu Trp Lys Met Gln 420 425 430 Arg Asp Lys Glu Val Trp Ser Glu Glu Pro Leu Glu Phe Arg Pro Glu 435 440 445 Arg Phe Leu Thr Ser Asn Thr Glu Val Asp Leu Lys Gly Gln His Tyr 450 455 460 Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser 465 470 475 480 Phe Ala Val Gln Leu Met His Leu Val Leu Ala Arg Leu Leu His Gly 485 490 495 Phe Glu Met Thr Thr Pro Met Gly Glu Lys Val Asp Met Thr Glu Ser 500 505 510 Ala Gly Leu Ile Ser His Lys Ile Thr Pro Leu Glu Val Leu Ile Lys 515 520 525 Pro Leu Arg Val 530 <210> 248 <211> 510 <212> PRT <213> Eschscholzia californica <400> 248 Met Asp Ser Phe Leu Leu Ala Tyr Trp Val Pro Ile Ser Val Ala Ser 1 5 10 15 Ile Ile Ala Phe Val Phe Leu Tyr Asn Leu Phe Ser Ser Arg Thr Leu 20 25 30 Gln Asn Lys Lys Ile Arg Thr Ala Pro Met Ala Thr Gly Ala Trp Pro 35 40 45 Ile Leu Gly His Leu His Leu Phe Gly Ser Gly Glu Leu Pro His Lys 50 55 60 Met Leu Ala Ala Met Ala Asp Lys Tyr Gly Ser Ala Phe Arg Met Lys 65 70 75 80 Phe Gly Lys His Thr Thr Leu Val Val Ser Asp Thr Arg Ile Val Lys 85 90 95 Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ser Asn Arg Pro Ser Thr 100 105 110 Lys Ala Phe Gln Leu Met Thr Tyr Asp Asn Glu Ser Val Ala Phe Thr 115 120 125 Pro Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys Ile Ser Thr Leu Lys 130 135 140 Leu Leu Ser Asn His Arg Leu Gln Ala Ile Lys Asp Val Arg Ala Ser 145 150 155 160 Glu Val Asn Val Cys Phe Lys Thr Leu Tyr Asp Gln Cys Lys Asn Pro 165 170 175 Ser Gly Ser Ala Pro Ile Leu Ile Asp Met Lys Lys Trp Phe Glu Glu 180 185 190 Val Ser Asn Asn Val Val Met Arg Val Ile Val Gly Arg Gln Asn Phe 195 200 205 Gly Ser Lys Ile Val Gln Gly Glu Glu Glu Ala Ile His Tyr Lys Lys 210 215 220 Val Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Met Phe Ser Asp 225 230 235 240 Phe Ala Pro Leu Leu Gly Phe Val Asp Ile Phe Gln Gly Asn Leu Ser 245 250 255 Ala Met Lys Arg Asn Ala Lys Lys Val Asp Ala Ile Leu Glu Asn Trp 260 265 270 Leu Glu Glu His Arg Lys Lys Lys Asn Ser Val Ala Glu Ser Gln Gln 275 280 285 Asp Phe Met Asp Val Met Leu Ser Ile Val Glu Glu Ser Lys Leu Ser 290 295 300 Gly His Asp Ala Asp Ala Val Ile Lys Ala Thr Cys Leu Ala Met Ile 305 310 315 320 Met Gly Gly Thr Asp Thr Thr Ala Val Ser Leu Thr Trp Ile Ile Ser 325 330 335 Leu Leu Met Asn Asn Arg His Ala Leu Lys Lys Ala Arg Glu Glu Leu 340 345 350 Asp Ala Leu Val Gly Lys Asp Arg Gln Val Glu Asp Ser Asp Leu Lys 355 360 365 Asn Leu Val Tyr Met Asn Ala Ile Val Lys Glu Thr Met Arg Met Tyr 370 375 380 Pro Leu Gly Thr Leu Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu Ile 385 390 395 400 Asp Gly Phe His Val Lys Gly Gly Thr Arg Leu Leu Val Asn Val Trp 405 410 415 Lys Leu Gln Arg Asp Pro Asn Val Trp Val Asp Pro Thr Glu Phe Arg 420 425 430 Pro Glu Arg Phe Leu Thr Glu Asn Ala Asp Ile Asp Val Gly Gly Gln 435 440 445 His Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly 450 455 460 Val Ser Phe Ala Leu Gln Leu His Ala Phe Ser Thr Cys Ser Pro His 465 470 475 480 Pro Trp Ile Arg Phe Glu Tyr Ser Lys Arg Arg Lys Cys Gly Ser Asp 485 490 495 Gly Glu Pro Arg Arg Thr Cys Glu Pro Gln Ser Ile Ala Ser 500 505 510 <210> 249 <211> 534 <212> PRT <213> Glaucium flavum <400> 249 Met Glu Phe Leu Ser Leu Gln Leu Gln Pro Val Ser Ile Phe Ala Leu 1 5 10 15 Leu Val Ala Ser Ile Phe Leu Tyr Asn Phe Leu Ile His Gly Lys Lys 20 25 30 Ser Asn Asn Lys Lys Thr Thr Lys Pro Pro Ala Pro Glu Ala Ser Gly 35 40 45 Gly Trp Pro Ile Met Gly His Leu His Leu Phe Asn Glu Asn Glu Leu 50 55 60 Thr His Arg Thr Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Ala Phe 65 70 75 80 Asn Ile Arg Phe Gly Ser His Pro Thr Leu Val Val Ser Ser Trp Asp 85 90 95 Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Arg Phe Phe Ser Asn Arg 100 105 110 Pro Gly Ser Leu Ala Ile Lys Leu Met Phe Tyr Asp Ala Asp Ser Val 115 120 125 Gly Tyr Ala Pro Tyr Gly Ala Tyr Trp Arg Asp Leu Arg Lys Ile Ser 130 135 140 Thr Leu Lys Leu Leu Ser Asn His Arg Leu Glu Thr Leu Lys His Leu 145 150 155 160 Arg Thr Ser Glu Val Glu Ser Cys Phe Lys Glu Leu Tyr Asn Gln Trp 165 170 175 Arg Asn Asn Lys Thr Gly Gly Gly Gly Asp Gly Phe Ala Pro Val Arg 180 185 190 Met Asp Asn Trp Phe Gly Asp Leu Thr Phe Asn Val Val Ala Arg Ile 195 200 205 Val Ala Gly Lys Lys Asn Phe Ala Gly Gly Ala Ala Ser Gly Asp Ala 210 215 220 Gly Ala Gln Arg Tyr Lys Glu Ala Met Asp Glu Ala Phe Arg Leu Met 225 230 235 240 Thr Ile Phe Ala Phe Ser Asp Val Val Pro Ala Leu Gly Trp Leu Asp 245 250 255 Lys Leu Arg Gly Leu Val Gly Gly Met Lys Arg Cys Gly Ala Glu Ile 260 265 270 Asp Ser Ile Val Ala Gly Trp Val Asp Glu His Arg Leu Lys Arg Ser 275 280 285 Ser Gly Lys Gly Ser Asp Ala Asp Leu Glu Gln Asp Phe Ile Asp Val 290 295 300 Cys Leu Glu Ile Met Glu His Ser Thr Leu Pro Gly Asp Asp Pro Glu 305 310 315 320 Val Val Ile Lys Ser Thr Cys Leu Asp Met Ile Leu Gly Gly Ser Asp 325 330 335 Thr Thr Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn 340 345 350 Pro His Val Leu Lys Arg Ala Arg Glu Glu Leu Asp Thr Asn Val Gly 355 360 365 Lys Asp Arg Gln Val Asp Asp Ser Asp Ile Pro Asn Leu Val Tyr Ile 370 375 380 Gln Ala Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu 385 390 395 400 Ile Glu Arg Arg Thr Ser Glu Asp Cys Glu Val Gly Gly Phe His Val 405 410 415 Pro Ala Gly Thr Arg Leu Leu Val Asn Leu Trp Lys Met Gln Arg Asp 420 425 430 Gly Ser Val Tyr Lys Glu Asp Pro Leu Glu Phe Ser Pro Glu Arg Phe 435 440 445 Leu Thr Ser Asn Ala Asp Val Asp Leu Lys Gly Gln Asn Tyr Glu Leu 450 455 460 Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser Phe Ala 465 470 475 480 Val Gln Leu Met His Leu Val Leu Ala Arg Leu Ile His Gly Phe Glu 485 490 495 Met Lys Thr Pro Glu Gly Gly Lys Val Asp Met Thr Glu Ser Ala Gly 500 505 510 Leu Ile Ser His Lys Val Thr Pro Leu Glu Val Leu Leu Lys Pro Arg 515 520 525 Leu Ala Ile Gln His Ser 530 <210> 250 <211> 561 <212> PRT <213> Glaucium flavum <400> 250 Met His Ala Lys Leu Thr Leu Ile Lys Lys Met Glu Phe Thr Ile Ile 1 5 10 15 Asn Ser Leu Glu Ile Gln Pro Ile Ile Ser Thr Phe Ala Leu Leu Thr 20 25 30 Phe Ser Ile Leu Leu Tyr Lys Ile Leu Leu Asn His Gly Arg Glu Asn 35 40 45 Lys Asn Asn Lys Pro Lys Thr Ser Ser Ser Ser Ser Ser Ile Pro Glu 50 55 60 Val Ala Gly Ala Trp Pro Ile Met Gly His Leu His Leu Phe Asn Gly 65 70 75 80 Asp Glu Leu Met His His Lys Leu Gly Ser Met Ala Glu Lys Tyr Gly 85 90 95 Gln Ala Phe Tyr Ile Arg Phe Gly Ser His Lys Ala Val Val Val Ser 100 105 110 Asn Trp Glu Met Val Lys Thr Cys Phe Thr Thr Asn Asn Gln Ile Phe 115 120 125 Leu Asn Arg Pro Pro Met Leu Ala Ile Asn Leu Leu Phe Phe Pro Thr 130 135 140 Asp Ser Leu Ser Tyr Ile Pro Tyr Gly Asp His Trp Arg Glu Leu Arg 145 150 155 160 Lys Phe Ser Asn Gln Lys Leu Leu Ser Asn His Arg Ile Glu Thr Gln 165 170 175 Lys Asn Leu Arg Lys Leu Glu Val Asp Tyr Cys Phe Lys Gln Leu Cys 180 185 190 Asn Gln Ser Ser Lys Tyr Phe Ile Ile Asn Asn Met Asp Asp Gln Asp 195 200 205 Ser Lys Phe Ala Leu Val Arg Met Asp Thr Trp Phe Asp Asp Val Thr 210 215 220 Leu Asn Val Leu Ala Arg Ile Ile Ala Gly Lys Lys Lys Phe Ile Ser 225 230 235 240 Gly Gly Ala Thr Ser Ser Gly Asp Asp Asp Asn Ala Glu Ala Arg Arg 245 250 255 Tyr Met Glu Ala Leu Asp Glu Gly Leu Arg Leu Met Thr Ser Phe Thr 260 265 270 Phe Ser Asp Val Leu Pro Trp Leu Gly Trp Leu Asp Asn Leu Arg Gly 275 280 285 Leu Ala Gly Lys Met Lys Arg Cys Gly Ala Glu Leu Asp Ser Val Phe 290 295 300 Ala Gly Trp Val Glu Glu His Arg Val Lys Arg Gly Ser Arg Lys Asp 305 310 315 320 Gly Asp Asp Ala Asp Leu Glu Gln Asp Phe Ile Asp Leu Cys Trp Glu 325 330 335 Ser Leu Glu Gln Val Pro Gly Asn Asp Pro Ala Lys Ile Ile Lys Leu 340 345 350 Ile Cys Met Glu Met Ile Leu Asn Gly Ser Gly Ala Thr Ala Val Thr 355 360 365 Leu Thr Trp Thr Val Ser Leu Leu Leu Asn Asn Pro Asp Val Leu Lys 370 375 380 Arg Ala Arg Glu Glu Leu Asp Thr His Val Gly Ser His Arg Gln Val 385 390 395 400 Asp Glu Ser Asp Ile Pro Asn Leu Val Tyr Ile Gln Ala Ile Ile Lys 405 410 415 Glu Gly Met Arg Leu Tyr Pro Pro Gly Pro Phe Leu Glu Arg Ser Thr 420 425 430 Thr Glu Asp Phe Glu Ile Asp Gly Val His Val Pro Ala Gly Thr Arg 435 440 445 Leu Trp Val Asn Leu Trp Lys Met His Arg Asp Glu Ser Met Tyr Gln 450 455 460 Glu Pro Leu Glu Phe Lys Pro Glu Arg Phe Leu Asn Ser Asn Ser Asp 465 470 475 480 Val Asp Leu Lys Gly Gln Ser Tyr Gln Leu Leu Pro Phe Gly Ala Gly 485 490 495 Arg Arg Ile Cys Pro Gly Val Ser Phe Ala Leu Pro Leu Met His Leu 500 505 510 Thr Leu Ala Arg Leu Ile His Gly Phe Glu Met Lys Leu Pro Val Gly 515 520 525 Val Glu Lys Val Asp Met Thr Glu Asn Gly Gly Ile Ile Asn Arg Lys 530 535 540 Ala Thr His Leu Asp Val Leu Leu Lys Pro Arg Leu Ile Ala Gln Gln 545 550 555 560 Ala <210> 251 <211> 536 <212> PRT <213> Glaucium flavum <400> 251 Met Asp Thr Leu Ser Ile Gln Trp Ile Val Pro Ser Ile Ala Thr Leu 1 5 10 15 Leu Ala Leu Val Phe Leu Tyr Asn Leu Ile Phe Thr Ser Lys Lys Thr 20 25 30 Thr Lys Thr Asn Asn Thr Arg Lys Ala Pro Met Ala Ser Gly Ala Trp 35 40 45 Pro Val Leu Gly His Leu His Leu Phe Gly Thr Gly Glu Leu Pro His 50 55 60 Lys Met Leu Ala Thr Met Ala Glu Asn Tyr Gly Thr Ala Phe Thr Met 65 70 75 80 Lys Phe Gly Asn His Thr Thr Leu Val Val Ser Asp Thr Arg Ile Val 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ala Asn Arg Pro Ser 100 105 110 Thr Lys Ala Phe Asp Leu Met Thr Tyr Ala Asn Asp Ser Val Ala Phe 115 120 125 Thr Pro Tyr Ser Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr Leu 130 135 140 Lys Leu Leu Ser Asn His Arg Leu Gln Ser Ile Lys Asp Val Arg Val 145 150 155 160 Ala Glu Val Asn Val Cys Phe Arg Gly Leu His Val Leu Cys Lys Ser 165 170 175 Lys Ile Tyr Gly Ala Pro Val Leu Val Asp Met Lys Lys Trp Phe Glu 180 185 190 Glu Val Ser Asn Asn Ile Val Met Arg Val Ile Val Gly Lys Gln Asn 195 200 205 Phe Gly Ser Arg Ile Val Gln Gly Gln Glu Glu Ala Val Tyr Tyr Lys 210 215 220 Ser Val Met Asp Glu Leu Leu Arg Leu Ala Ser Val Ser Val Leu Ser 225 230 235 240 Asp Phe Ala Pro Leu Phe Gly Trp Leu Asp Phe Phe Gln Gly Asn Ile 245 250 255 Ser Ala Met Lys Arg Asn Gly Lys Lys Leu Asp Val Ile Leu Glu Arg 260 265 270 Trp Met Glu Glu His Arg Gln Lys Lys Ile Ser Ser Ser Ser Ser Ile 275 280 285 Ala Ala Ser Gly Ala Gly Glu Asp Asp Glu Gln Asp Phe Met Asp Val 290 295 300 Met Leu Ser Ile Ile Glu Glu Thr Lys Leu Ser Gly Arg Asp Ala Asp 305 310 315 320 Thr Val Ile Lys Ala Thr Cys Leu Ala Met Ile Met Gly Gly Thr Asp 325 330 335 Thr Thr Ala Val Ser Leu Thr Trp Ile Val Ser Leu Leu Met Asn Asn 340 345 350 Arg His Val Leu Lys Lys Ala Arg Glu Glu Leu Asp Ser Leu Val Gly 355 360 365 Lys Asp Arg Gln Val Glu Asp Ser Asp Leu Lys Asn Phe Val Tyr Met 370 375 380 Asn Ala Ile Val Lys Glu Thr Met Arg Leu Tyr Pro Phe Gly Ala Leu 385 390 395 400 Leu Glu Arg Asp Thr Lys Glu Asp Cys Glu Val Gly Gly Phe His Val 405 410 415 Glu Ala Gly Thr Arg Leu Leu Val Asn Val Trp Lys Leu Gln Arg Asp 420 425 430 Pro Asn Val Trp Lys Asp Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu 435 440 445 Val Glu Asn Val Asp Ile Asp Val Gly Gly Gln His Phe Glu Leu Leu 450 455 460 Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala Leu 465 470 475 480 Gln Phe Met His Leu Val Leu Ala Arg Leu Ile His Gly Tyr Glu Leu 485 490 495 Glu Thr Leu Asn Gly Glu Asp Val Asp Leu Thr Glu Ser Thr Glu Gly 500 505 510 His Val Asn His Lys Ala Ser Pro Leu Asp Leu Leu Ile Thr Pro Arg 515 520 525 Leu Asp Ser Lys Val Tyr Asn Tyr 530 535 <210> 252 <211> 532 <212> PRT <213> Glaucium flavum <400> 252 Met Asp Ser Thr Leu Val Leu Gln Cys Phe Val Gly Ser Met Ala Ala 1 5 10 15 Val Ser Ala Leu Val Phe Leu Tyr Asn Leu Ile Phe Ser Ser Ser Lys 20 25 30 Thr Thr Lys Gly Lys Val Leu Arg Lys Ala Pro Met Ala Ala Gly Ala 35 40 45 Trp Pro Ile Leu Gly His Leu His Leu Phe Gly Ser Gly Glu Leu Pro 50 55 60 His Lys Met Leu Ser Lys Met Ala Glu Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Leu Lys Phe Gly Lys His Thr Thr Leu Val Val Ser Asp Thr Arg Val 85 90 95 Val Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ala Asn Arg Pro 100 105 110 Ser Thr Thr Ala Phe Asp Leu Met Thr Tyr Ala Asn Asp Ser Val Ala 115 120 125 Phe Thr Pro Tyr Ser Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ser Ile Lys Glu Ile Arg 145 150 155 160 Val Ser Glu Val Asn Val Cys Phe Arg Glu Leu Phe Glu Met Ser Lys 165 170 175 Ser Lys Thr Asp Gly Ala Ala Pro Ala Leu Val Asp Met Lys Lys Trp 180 185 190 Phe Glu Glu Val Ser Asn Asn Ile Val Met Arg Val Ile Val Gly Arg 195 200 205 Gln Asn Phe Gly Ser Lys Ile Val Gln Gly Asp Ala Glu Ala Val Asn 210 215 220 Tyr Lys Asn Val Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Met 225 230 235 240 Leu Ser Asp Phe Ala Pro Leu Leu Gly Trp Val Asp Met Phe Gln Gly 245 250 255 Asn Lys Asn Ala Met Lys Arg Asn Ala Lys Lys Val Asp Thr Ile Leu 260 265 270 Glu Gly Trp Leu Glu Glu His Arg Lys Lys Asn Lys Lys Met Ser Ser 275 280 285 Ser Glu Asn Asp Glu Gln Asp Phe Met Asp Val Met Leu Ser Ile Ile 290 295 300 Glu Glu Thr Lys Leu Ser Gly Arg Asp Ala Asp Thr Val Ile Lys Ala 305 310 315 320 Thr Val Leu Ala Met Ile Met Gly Gly Thr Asp Thr Thr Ala Val Ser 325 330 335 Leu Thr Trp Ile Val Ser Leu Leu Met Asn Asn Arg His Val Leu Lys 340 345 350 Lys Ala Arg Glu Glu Ile Asp Ala Ile Val Gly Lys Asp Arg Gln Val 355 360 365 Glu Asp Ser Asp Leu Lys Asn Phe Val Tyr Met Asn Ala Ile Val Lys 370 375 380 Glu Thr Met Arg Leu Tyr Pro Leu Gly Ala Met Leu Glu Arg Asp Thr 385 390 395 400 Lys Glu Asp Cys Glu Val Gly Gly Phe Gln Val Gln Ala Gly Thr Arg 405 410 415 Leu Leu Val Asn Val Trp Lys Leu Gln Arg Asp Pro Asn Val Trp Ser 420 425 430 Asp Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Ser Glu Asn Ala Asp 435 440 445 Ile Asp Val Gly Gly Gln His Phe Glu Leu Leu Pro Phe Gly Ala Gly 450 455 460 Arg Arg Val Cys Pro Arg Val Ser Phe Ala Leu Gln Phe Met His Leu 465 470 475 480 Val Leu Ala Arg Leu Ile His Gly Tyr Glu Leu Gly Thr Gln Asn Asp 485 490 495 Glu Leu Val Asp Leu Thr Glu Ser Thr Glu Gly His Val Asn His Met 500 505 510 Ala Ser Pro Leu Asp Leu Ile Leu Thr Pro Arg Leu Ser Asn Pro Lys 515 520 525 Leu Tyr Asp Tyr 530 <210> 253 <211> 518 <212> PRT <213> Glaucium flavum <400> 253 Met Phe Phe Thr Ser Ser Ser Lys Thr Thr Asn Lys Asn Thr Ser Lys 1 5 10 15 Lys Pro Pro Met Ala Pro Gly Ala Trp Pro Ile Leu Gly His Leu His 20 25 30 Leu Phe Lys Glu Gly Glu Leu Pro His His Met Leu Lys Ser Met Ala 35 40 45 Asp Lys Tyr Gly Pro Ala Phe Leu Met Lys Phe Gly Gln His Arg Ser 50 55 60 Leu Val Val Ser Asp Tyr Arg Ile Val Lys Glu Cys Phe Thr Thr Asn 65 70 75 80 Asp Thr Leu Phe Ser Asn Arg Pro Ser Thr Thr Ala Phe Ala Val Met 85 90 95 Thr Tyr Ala Thr Asp Ser Val Ala Phe Thr Glu Tyr Ser Pro Tyr Trp 100 105 110 Arg Glu Leu Arg Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn Asn Arg 115 120 125 Leu Gln Ala Ile Lys Lys Leu Arg Glu Ser Glu Val Asn Val Cys Phe 130 135 140 Arg Gly Leu Tyr Asp Ser Trp Arg Lys Asn Lys Ser Glu Gln Asn Gly 145 150 155 160 Ala Gly Asn Ser Ile Asp Gly Gly Asn Glu Arg Ala Arg Pro Val Leu 165 170 175 Val Asp Met Lys Lys Trp Phe Glu Glu Val Ser Asn Asn Leu Val Met 180 185 190 Arg Val Ile Val Gly Lys Arg Asn Phe Gly Thr Lys Ile Val Glu Gly 195 200 205 Glu Lys Glu Ala Val Glu Tyr Lys Thr Ile Met Asp Glu Leu Leu Arg 210 215 220 Leu Ala Ser Leu Ser Leu Leu Ser Asp Phe Ala Pro Ile Leu Arg Leu 225 230 235 240 Phe Asp His Phe Gln Gly His Ile Arg Thr Met Lys Arg Asn Gly Lys 245 250 255 Lys Leu Asp Val Leu Leu Gln Arg Trp Leu Glu Glu His Arg Arg Lys 260 265 270 Met Ser Thr Pro Glu Glu Glu Gln Asp Phe Met Asp Val Met Leu Ser 275 280 285 Ile Val Asp Glu Ser Lys Leu Ser Gly His Asp Ala Asp Thr Val Ile 290 295 300 Lys Ala Thr Cys Leu Ala Met Ile Met Gly Gly Thr Asp Thr Ser Ala 305 310 315 320 Val Ser Leu Thr Trp Ile Val Ser Leu Leu Met Asn Asn Arg His Ala 325 330 335 Leu Ala Lys Ala Arg Glu Glu Leu Asp Lys His Val Gly Lys Asp Arg 340 345 350 Gln Val Glu Glu Ser Asp Leu Lys Asn Leu Val Tyr Leu His Ala Ile 355 360 365 Val Lys Glu Thr Met Arg Leu Tyr Pro Leu Gly Pro Leu Leu Glu Arg 370 375 380 Glu Thr Lys Gln Asp Cys Glu Val Gly Gly Phe Asp Ile Ala Gly Gly 385 390 395 400 Thr Arg Ile Leu Val Asn Ile Trp Met Val Gln Arg Asp Pro Ala Val 405 410 415 Trp Asn Asp Ala Thr Glu Phe Ile Pro Glu Arg Phe Leu Thr Glu Lys 420 425 430 Ser Asp Val Asp Val Trp Gly Gly Ser Phe Glu Leu Ile Pro Phe Gly 435 440 445 Ala Gly Arg Arg Val Cys Pro Gly Val Ser Phe Ala Leu Gln Phe Leu 450 455 460 His Leu Val Leu Ala Arg Leu Ile His Gly Tyr Glu Leu Lys Thr Pro 465 470 475 480 Asn Asp Met Pro Val Asp Leu Thr Glu Ser Thr Glu Gly His Val Asn 485 490 495 His Lys Ala Ser Pro Leu Asp Leu Leu Leu Val Pro Arg Leu Ser Asp 500 505 510 Leu Lys Leu Tyr Asp Tyr 515 <210> 254 <211> 537 <212> PRT <213> Glaucium flavum <400> 254 Met Asp Ser Ile Leu Thr Thr Val Val Leu Leu Ser Ile Leu Leu Tyr 1 5 10 15 Phe Leu Phe Ser Trp Gln Leu Asn Lys Tyr Ser Ala Thr Lys Lys Asn 20 25 30 Thr Lys Ser Ser Lys Gln Leu Pro Pro Glu Pro Ala Gly Ser Trp Pro 35 40 45 Val Ile Gly His Leu His Leu Leu Ala Lys Gly Ser Asn Leu Pro His 50 55 60 Ile Asn Leu Gly Ala Met Ala Asp Lys Tyr Gly Pro Ile Phe Met Ile 65 70 75 80 Arg Ile Gly Leu Asn Pro Thr Leu Val Val Ser Ser Trp Glu Val Ala 85 90 95 Arg Glu Ile Phe Thr Thr Asn Asp Gln Val Phe Ser Ser Arg Pro Ile 100 105 110 Gln Val Ser Thr Lys His Leu Gly Phe Asp Thr Ala Met Tyr Gly Phe 115 120 125 Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Ile Ser Lys Leu Val Lys Arg 130 135 140 Glu Val Leu Ser Asn Thr Arg Leu Glu Phe Leu Lys Pro Val Trp Gly 145 150 155 160 Ser Glu Ile Asn Thr Ser Ile Lys Glu Leu Tyr Asp Val Cys Val Met 165 170 175 Lys Asn Lys Glu Glu Gly Gly Thr Gly Pro Ile Ile Val Glu Met Lys 180 185 190 Gln Trp Phe Ser Asp Leu Ala Leu Asn Met Ser Val Lys Leu Val Ala 195 200 205 Gly Lys Arg Tyr Phe Gly Ala Ser Gln Leu Gly Asn Glu Glu Ala Ala 210 215 220 Arg Trp Gln Lys Ala Leu Arg Asn Cys Phe Arg Leu Val Gly Leu Phe 225 230 235 240 Val Val Ser Asp Ala Ile Pro Phe Leu Arg Trp Leu Asp Val Gly Gly 245 250 255 His Glu Lys Glu Met Lys Asn Thr Ala Lys Glu Leu Asp Asp Leu Leu 260 265 270 Glu Gly Trp Leu Glu Glu His Lys Met Lys Lys Lys Leu Ser Leu Ser 275 280 285 Glu Val Glu Ala Glu Lys Lys Glu Arg Asp Arg Val Asp Phe Met Asp 290 295 300 Val Met Leu Ser Thr Leu Glu His Glu Lys Ala Ser Asp Tyr Phe Pro 305 310 315 320 Ala Asp Thr Ile Asn Lys Ala Thr Cys Leu Ala Leu Ile Leu Gly Gly 325 330 335 Thr Asp Thr Thr Thr Val Val Trp Val Trp Ala Leu Ala Leu Leu Val 340 345 350 Asn Asn Pro Asn Val Leu Lys Lys Ala Gln Asp Glu Leu Asp Val His 355 360 365 Val Gly Lys Lys Arg Gln Val Asp Glu Ser Asp Ile Lys Asn Leu Thr 370 375 380 Tyr Leu Gln Ala Ile Ile Lys Glu Ser Met Arg Leu Tyr Pro Ala Ala 385 390 395 400 Thr Leu Gly Ile Arg Glu Ser Thr Glu Asp Cys Thr Val Ala Gly Tyr 405 410 415 His Ile Pro Ala Gly Thr Ser Leu Ile Val Asn Ser Trp Lys Ile Gln 420 425 430 His Asp Pro Gln Val Trp Thr Asp Pro Phe Glu Phe Gln Pro Glu Arg 435 440 445 Phe Leu Thr Gly His Met Asp Val Asp Ile Arg Gly Gln Ser Cys Lys 450 455 460 Phe Leu Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Thr Ser Leu 465 470 475 480 Ala Leu Gln Met Val Thr Leu Thr Leu Ala Arg Leu Ile His Gly Phe 485 490 495 Glu Phe Arg Thr Pro Ser Glu Ala Pro Thr Asp Met Thr Glu Ser Ala 500 505 510 Gly Leu Thr Asn Val Lys Ala Thr Pro Leu Glu Val Leu Val Ser Pro 515 520 525 Arg Leu Pro Ser Glu Leu Tyr Val Cys 530 535 <210> 255 <211> 537 <212> PRT <213> Glaucium flavum <400> 255 Met Glu Leu Ile Asn Ser Leu Glu Ile Gln Pro Ile Thr Ile Ser Ile 1 5 10 15 Leu Ala Leu Leu Thr Val Ser Ile Leu Leu Tyr Lys Ile Ile Trp Asn 20 25 30 His Gly Ser Arg Lys Asn Asn Lys Ser Asn Lys Asn Asn Arg Lys Thr 35 40 45 Ser Ser Ser Ala Gly Val Val Glu Ile Pro Gly Ala Trp Pro Ile Ile 50 55 60 Gly His Leu His Leu Phe Asn Gly Ser Glu Gln Met Phe His Lys Leu 65 70 75 80 Gly Ser Leu Ala Asp Gln Tyr Gly Pro Ala Pro Phe Phe Ile Arg Phe 85 90 95 Gly Ser Arg Lys Tyr Val Val Val Ser Asn Trp Glu Leu Val Lys Thr 100 105 110 Cys Phe Thr Ala Gln Ser Gln Ile Phe Val Ser Arg Pro Pro Met Leu 115 120 125 Ala Met Asn Ile Leu Phe Phe Pro Lys Asp Ser Leu Ser Tyr Ile Gln 130 135 140 His Gly Asp His Trp Arg Glu Leu Arg Lys Ile Ser Ser Thr Lys Leu 145 150 155 160 Leu Ser Ser His Arg Val Glu Thr Gln Lys His Leu Ile Ala Ser Glu 165 170 175 Val Asp Tyr Cys Phe Lys Gln Leu Tyr Lys Leu Ser Asn Asn Gly Glu 180 185 190 Phe Thr Leu Val Arg Leu Asn Thr Trp Cys Glu Asp Met Ala Leu Asn 195 200 205 Val His Val Arg Met Ile Ala Gly Met Lys Asn Tyr Val Ala Ala Pro 210 215 220 Gly Ser Gly Glu Tyr Gly Gly Gln Ala Arg Arg Tyr Arg Lys Ala Leu 225 230 235 240 Glu Glu Ala Leu Asp Leu Leu Asn Gln Phe Thr Ile Thr Asp Val Val 245 250 255 Pro Trp Leu Gly Trp Leu Asp His Phe Arg Asp Val Val Gly Arg Met 260 265 270 Lys Arg Cys Gly Ala Glu Leu Asp Ser Ile Phe Ala Thr Trp Val Glu 275 280 285 Glu His Arg Val Lys Arg Ala Ser Gly Lys Gly Gly Asp Val Glu Pro 290 295 300 Asp Phe Ile Asp Leu Cys Trp Glu Ser Met Glu Gln Leu Pro Gly Asn 305 310 315 320 Asp Pro Ala Thr Val Ile Lys Leu Met Cys Lys Glu His Ile Phe Asn 325 330 335 Gly Ser Gly Thr Ser Ser Leu Thr Leu Ala Trp Ile Leu Ser Leu Ile 340 345 350 Met Asn Asn Pro Tyr Val Ile Lys Lys Ala Arg Glu Glu Leu Glu Lys 355 360 365 His Val Gly Asn His Arg Gln Val Glu Glu Ser Asp Leu Pro Asn Leu 370 375 380 Leu Tyr Ile Gln Ala Ile Ile Lys Glu Gly Met Arg Leu Tyr Thr Pro 385 390 395 400 Gly Pro Phe Ile Asp Arg Asn Thr Thr Glu Asp Tyr Glu Ile Asn Gly 405 410 415 Val His Ile Pro Ala Gly Thr Cys Leu Tyr Val Asn Leu Trp Lys Ile 420 425 430 His Arg Asp Pro Asn Val Tyr Glu Asp Pro Leu Glu Phe Lys Pro Glu 435 440 445 Arg Phe Leu Lys Asn Asn Ser Asp Leu Asp Leu Lys Gly Gln Asn Tyr 450 455 460 Gln Leu Leu Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser 465 470 475 480 Leu Ala Leu Pro Leu Met Tyr Leu Thr Val Ser Arg Leu Ile His Gly 485 490 495 Phe Asp Met Lys Leu Pro Lys Gly Val Glu Lys Ala Asp Met Thr Ala 500 505 510 His Gly Gly Val Ile Asn Gln Arg Ala Tyr Pro Leu Glu Val Leu Leu 515 520 525 Lys Pro Arg Leu Thr Phe Gln Gln Ala 530 535 <210> 256 <211> 571 <212> PRT <213> Glaucium flavum <400> 256 Met Asp Leu Gln Ile Phe Phe His Phe Gln Gly Ile Val Gly Ser Leu 1 5 10 15 Ala Leu Leu Ser Phe Phe Tyr Tyr Leu Trp Arg Leu Leu Thr Thr Thr 20 25 30 Lys Thr Ser Ile Cys Asn Gly Thr Thr Ala Ala Pro Pro Glu Val Ser 35 40 45 Gly Gly Trp Pro Ile Leu Gly His Leu Leu Gln Leu Val Gly Ser Lys 50 55 60 Gln Pro Leu Phe Lys Val Leu Gly Asp Met Ala Asp Lys Tyr Gly Pro 65 70 75 80 Ile Phe Val Val Arg Phe Gly Met Tyr Pro Thr Leu Val Val Ser Ser 85 90 95 Trp Glu Met Ala Lys Glu Cys Phe Ser Thr Asn Asp Arg Val Leu Ala 100 105 110 Thr Arg Pro Thr Ser Ala Ala Ser Lys Tyr Leu Thr Tyr Asn Tyr Ala 115 120 125 Met Phe Ala Phe Thr Phe Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys 130 135 140 Ile Ser Thr Ile Glu Leu Leu Ser His Arg Arg Val Glu Met Phe Lys 145 150 155 160 His Val Pro Phe Met Glu Ile Asp Thr Cys Ile Glu Gln Leu Tyr Leu 165 170 175 Leu Trp Met Gln Asn Gln Asn Gln Asn Gln Asn Gln Pro Asn Gly Asp 180 185 190 Pro Val Gln Val Asn Met Ser Lys Val Phe Glu Glu Leu Thr Met Asn 195 200 205 Ala Val Leu Lys Leu Val Val Gly Lys Arg Leu Thr Asp Asp Lys Glu 210 215 220 Gly Glu Lys Leu His Lys Thr Ile Gln Glu Phe Phe Lys Leu Leu Glu 225 230 235 240 Val Ser Val Ala Ser Asp Val Phe Pro Phe Leu Gly Trp Leu Asp Val 245 250 255 Asp Gly Gln Lys Arg Lys Met Lys Arg Val Ala Lys Glu Met Asp Ile 260 265 270 Ile Ala Glu Lys Trp Leu Glu Glu His Arg Gln Lys Arg Ser Ser Lys 275 280 285 Leu Glu Glu Glu Glu Glu Glu Glu Glu Asp Asp Gly Gly Gly Lys Gly 290 295 300 Asp Ala Ala Ala Ala Asp Lys Asp Phe Met Asp Val Leu Leu Ser Leu 305 310 315 320 Leu Glu Gly Asp Glu Gly Asp Ser Asp Gln Pro Phe Met Asn Tyr Ser 325 330 335 Arg Asp Thr Val Ile Lys Ala Thr Ser Leu Asn Ile Leu Val Ala Ala 340 345 350 Thr Asp Thr Thr Ser Pro Thr Leu Thr Trp Ala Ile Ser Leu Leu Leu 355 360 365 Asn Asn Pro His Val Leu Arg Glu Ala Gln Asn Glu Leu Asp Met Lys 370 375 380 Val Gly Arg Asp Arg Gln Val Glu Glu Gln Asp Ile Glu Asn Leu Ile 385 390 395 400 Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Ala Gly 405 410 415 Pro Leu Ser Ile Pro His Glu Ala Ile Gln Asp Cys Lys Leu Gly Gly 420 425 430 Tyr His Val Arg Ala Gly Thr Arg Leu Leu Leu Asn Ile Trp Lys Leu 435 440 445 His Arg Asp Pro Arg Val Trp Ser Asn Pro Leu Glu Phe Lys Pro Glu 450 455 460 Arg Phe Leu Ile Leu Ser Glu Glu Val Cys Gly Cys Ser Arg Gly Thr 465 470 475 480 Gln Asn Phe Asp Phe Lys Gly Gln Cys Phe Glu Tyr Ile Pro Phe Gly 485 490 495 Ser Gly Arg Arg Met Cys Pro Gly Tyr Asn Phe Gly Ile Gln Ile Ile 500 505 510 His Met Thr Leu Ala Arg Leu Leu Gln Ser Phe Glu Met Gln Pro Ala 515 520 525 Lys Ala Lys Ser Leu Asn Asp Gln Asp Gly Pro Val Asp Met Arg Glu 530 535 540 Gly Ser Gly Leu Thr Leu Pro Lys Ile Thr Pro Leu Lys Val Leu Leu 545 550 555 560 Thr Pro Arg Leu Tyr Gly Gln Leu Tyr Asn His 565 570 <210> 257 <211> 534 <212> PRT <213> Hydrastis canadensis <400> 257 Met Asp Phe Ser Met Leu Phe Gln Trp Leu Leu Val Thr Met Ala Thr 1 5 10 15 Leu Leu Phe Leu Asn Ser Val Tyr Asn Val Trp Tyr Lys Ser Ser Lys 20 25 30 Asn Thr Ser Ile Thr Thr Thr Thr Ser Lys Gly Lys Lys Ala Pro Val 35 40 45 Ala Ala Gly Ala Arg Pro Phe Met Gly His Leu His Met Leu Val Gly 50 55 60 Gly Lys Gln Leu Pro His Gln Ala Leu Gly Lys Leu Ala Asp Ile Tyr 65 70 75 80 Gly Pro Ala Phe Ile Ile His Ile Gly Pro Asn Pro Glu Leu Val Val 85 90 95 Ser Ser Trp Glu Leu Ala Lys Glu Cys Phe Thr Thr Asn Asp Lys Tyr 100 105 110 Phe Ala Asn Arg Pro Thr Asn Lys Ala Met Lys Tyr Leu Thr Tyr Asp 115 120 125 Glu Ala Ser Val Gly Phe Gly Pro Tyr Gly Pro Leu Trp Val Glu Met 130 135 140 Arg Lys Ile Ala Lys Ser Asn Leu Leu Ser Gln Gln Arg Leu Gln Met 145 150 155 160 His Lys Arg Val Arg Val Leu Glu Ile Asp Ala Phe Phe Lys Glu Leu 165 170 175 His Glu Leu Trp Ser Met Lys Lys Glu Asp Gly Pro Val Ser Val Asp 180 185 190 Met Lys Gln Trp Phe Glu Glu Leu Thr Leu Asn Val Ile Thr Arg Met 195 200 205 Val Ser Gly Lys His Lys Tyr Ala Thr Lys Ala Arg Arg Gly Asp Ser 210 215 220 Glu Ala Lys Gln Phe Lys Arg Val Ile Gly Glu Ala Ala His Phe Thr 225 230 235 240 Gly Asn Leu Leu Leu Ser Asp Ile Phe Pro Ser Leu Gly Phe Leu Asp 245 250 255 Asn Met Gln Gly Arg Val Asn Ser Met Lys Arg Thr Gly Lys Glu Leu 260 265 270 Asp Ser Ile Leu Ser Ser Trp Val Glu Glu His Arg Gln Lys Lys Leu 275 280 285 Ser Gly Glu Gln Ser Glu Asp Glu Glu Lys Asp Phe Ile Asp Leu Thr 290 295 300 Leu Ser Met Met Asp Glu Ile Gln Leu His Ser Thr Asp Ser Glu Thr 305 310 315 320 Phe Ile Lys Ala Ile Cys Val Gly Val Ile Leu Gly Gly Ser Asp Thr 325 330 335 Thr Ser Val Gly Ile Thr Trp Ile Leu Ser Leu Leu Met Asn His Arg 340 345 350 Asp Val Leu Lys Lys Ala Gln Glu Glu Leu Asp Gln Gln Val Gly Met 355 360 365 Asp Arg Lys Val Glu Asp Ser Asp Leu Asn Asn Leu Val Tyr Leu Arg 370 375 380 Ala Ile Val Lys Glu Ser Met Arg Leu Tyr Gln Val Gly Pro Leu Ile 385 390 395 400 Glu Arg Lys Ala Ser Gln Asp Cys Thr Ile Gly Gly Phe His Val Lys 405 410 415 Ala Gly Thr Arg Leu Leu Val Asn Leu Ser Lys Val His Lys Asp Pro 420 425 430 Thr Val Trp Ser Asp Pro Leu Glu Phe Gln Pro Glu Arg Phe Leu Thr 435 440 445 Thr His Ser Asn Met Asp Leu Lys Gly Gln His Phe Glu Leu Leu Pro 450 455 460 Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Tyr Leu Phe Ala Leu Asn 465 470 475 480 Glu Met Tyr Leu Val Leu Ala Arg Leu Ile Gln Gly Phe Glu Leu Gly 485 490 495 Thr Pro Met Asp Ala Lys Val Asp Met Thr Glu Thr Ser Ser Val Thr 500 505 510 Asn Tyr Arg Ala Thr Pro Leu Gln Val Leu Leu Thr Pro Arg Leu Ser 515 520 525 Pro Lys Leu Tyr Asp Tyr 530 <210> 258 <211> 528 <212> PRT <213> Hydrastis canadensis <400> 258 Met Ala Phe Val Ala Leu Ile Val Val Tyr Asn Ile Trp Phe Lys Ser 1 5 10 15 Ala Ala Ser Arg Asn Lys Thr Ser Tyr Asn Asn Lys Thr Met Ser Lys 20 25 30 Thr Pro Val Val Asp Gly Ala Lys Pro Ile Ile Gly His Leu His Leu 35 40 45 Leu Met Gly Gly Asp Leu Pro His His Ala Leu Gly Arg Leu Ala Asp 50 55 60 Lys Tyr Gly Pro Ile Phe Leu Met His Ile Gly Ala Phe Pro Glu Leu 65 70 75 80 Val Val Ser Ser Ser Glu Leu Ala Lys Glu Cys Phe Thr Thr Asn Asp 85 90 95 Lys Tyr Ile Ile Asn Arg Pro Thr Asn Lys Ala Met Thr Tyr Leu Ser 100 105 110 Tyr Glu Gln Ala Ser Val Gly Phe Ala Pro Tyr Gly Pro Leu Trp Ile 115 120 125 Glu Met Arg Lys Ile Ser Lys Ser Asn Leu Leu Ser Asn Gln Arg Ile 130 135 140 His Met Gln Lys Gln Val Arg Val Ala Glu Leu Asp Ala Phe Phe Lys 145 150 155 160 Glu Leu Tyr Gln Leu Cys Arg Ser Asn Asp Glu Asn Asn Asn Asn Ser 165 170 175 Thr Ser His Gly Lys Val Leu Val Glu Met Asn Lys Trp Phe Glu Glu 180 185 190 Leu Thr Leu Asn Val Val Thr Arg Met Ile Cys Gly Lys Gln Lys Met 195 200 205 Gly Thr Lys Ala Arg Leu Gly Asp Ser Asp Ala Lys His Tyr Lys Lys 210 215 220 Thr Ile Asp Glu Ala Ala His Phe Met Gly Asn Leu Val Ile Ser Asp 225 230 235 240 Val Val Pro Cys Leu Gly Met Leu Asp Asn Leu Leu Gly His Val Ser 245 250 255 Ala Met Lys Arg Thr Gly Lys Glu Leu Asp Thr Ile Phe Gly Ser Trp 260 265 270 Val Glu Glu His Gln Gln Lys Ile Arg Leu Ser Gly Tyr Lys Asp Asp 275 280 285 Ala Glu Glu Glu Glu His Asp Phe Ile Asp Leu Thr Leu Ser Met Met 290 295 300 Lys Gly Ser Thr Asp Leu His Gly Leu Asp Pro Ala Thr Phe Ile Lys 305 310 315 320 Ser Ile Cys Val Gly Met Ile Leu Gly Gly Thr Asp Thr Thr Ser Val 325 330 335 Ala Leu Thr Trp Ile Leu Ser Leu Leu Leu Asn Asn Arg His Ile Leu 340 345 350 Lys Lys Ala Gln Glu Glu Leu Asp His Gln Val Gly Lys Glu Arg Lys 355 360 365 Val Glu Asp Ser Asp Leu Asn Asn Leu Val Phe Ile Gly Ala Ile Val 370 375 380 Lys Glu Ser Met Arg Leu Tyr Pro Val Gly Pro Leu Ile Glu Arg Glu 385 390 395 400 Ala Ile Glu Asp Cys Gln Ile Gly Gly Val His Val Lys Ala Gly Thr 405 410 415 Arg Leu Leu Ile Asn Ile Trp Lys Val Gln Gln Asp Pro Lys Ile Trp 420 425 430 Pro Asn Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Asp Ser Asn Met 435 440 445 Asp Val Lys Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg 450 455 460 Arg Met Cys Pro Gly Met Ser Phe Ala Ile Asn Glu Leu Asn Leu Val 465 470 475 480 Leu Ala Arg Leu Leu Gln Gly Phe Glu Leu Glu Thr Pro Met Asn Ala 485 490 495 Lys Val Asp Met Thr Glu Thr Ser Ser Val Thr Asn Tyr Lys Gly Thr 500 505 510 Pro Leu Gln Val Leu Leu Thr Pro Arg Leu Ser Ser Lys Leu Tyr Met 515 520 525 <210> 259 <211> 531 <212> PRT <213> Hydrastis canadensis <400> 259 Met Glu Tyr Ser His Leu Leu Pro Trp Leu Ala Thr Ser Ile Ala Ala 1 5 10 15 Ile Phe Ala Phe Ile Phe Leu Phe Tyr Val Arg Arg Arg Lys Asn Asn 20 25 30 Lys Ala Phe Ile His Asn His Ala Lys Lys Ala Pro Val Val Pro Gly 35 40 45 Ala Leu Pro Phe Leu Gly His Leu Arg Leu Phe Thr Gly Pro Ile Leu 50 55 60 Pro His Lys Ala Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Ala Phe 65 70 75 80 Thr Ile Tyr Leu Gly Ser His Gln Thr Leu Val Val Ser Gly Arg Glu 85 90 95 Leu Val Lys Glu Cys Phe Thr Thr Asn Asp Arg Leu Phe Ser Asn Arg 100 105 110 Pro Arg Ser Lys Ala Val Lys Tyr Leu Thr Tyr Asp Glu Ala Ser Val 115 120 125 Gly Phe Ala Pro Tyr Gly Pro Leu Trp Val Glu Met Arg Lys Val Ser 130 135 140 Lys Leu Asn Phe Leu Ser Asn Gln Arg Ile Gln Met Gln Lys Gln Ala 145 150 155 160 Leu Ala Ser Glu Leu Asn Phe Cys Phe Lys Asp Val Tyr Gln Leu Trp 165 170 175 Leu Lys Asn Lys Glu Leu Pro Val Met Val Asp Met Thr Lys Trp Phe 180 185 190 Glu Glu Met Thr Leu Asn Val Ile Thr Arg Leu Ile Cys Gly Lys Gln 195 200 205 Asn Tyr Gly Ser Lys Ala Asn Ser Gly Glu Ser Glu Ala Lys Arg Tyr 210 215 220 Lys Gln Val Val Glu Lys Ala Ala His Leu Thr Ser Thr Val Val Met 225 230 235 240 Ser Asp Val Phe Pro Phe Leu Glu Trp Phe Asp Lys Phe Gln Gly Gln 245 250 255 Glu Lys Val Met Lys Lys Val Ala Asn Glu Phe Asp Ser Ile Leu Gly 260 265 270 Ser Trp Val Asp Glu His Arg Arg Lys Arg Leu Leu Arg Gly Asn Asn 275 280 285 Glu Glu Glu Glu Glu Glu Gln Asp Phe Ile Asp Leu Ser Leu Ala Met 290 295 300 Met Glu Glu Thr Gln Leu His Gly Val Asp Pro Asp Thr Phe Ile Lys 305 310 315 320 Ser Met Cys Leu Gly Met Ile Leu Gly Gly Gly Asp Thr Thr Pro Val 325 330 335 Ala Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Pro Asp Ile Met 340 345 350 Lys Lys Ala Gln Glu Glu Ile Asp Gln Val Ile Gly Lys Glu Arg Lys 355 360 365 Leu Asp Gly Ser Asp Ile Ser Asn Leu Val Tyr Leu Gln Ala Val Val 370 375 380 Lys Glu Ser Met Arg Leu Tyr Gln Val Gly Pro Leu Ile Glu Arg Glu 385 390 395 400 Thr Thr Glu Asp Cys Lys Ile Gly Asp Phe His Val Glu Ala Gly Thr 405 410 415 Arg Leu Leu Val Asn Ile Trp Lys Val Gln Gln Asp Pro Cys Val Trp 420 425 430 Ser Asn Pro Thr Glu Phe Gln Pro Glu Arg Phe Leu Ser Ser Lys Ser 435 440 445 Asp Met Asp Leu Lys Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ser 450 455 460 Gly Arg Arg Met Cys Pro Ala Val Ala Ser Ala Leu Gln Met Met His 465 470 475 480 Leu Val Leu Ala Lys Leu Ile His Gly Phe Glu Leu Gly Thr Pro Met 485 490 495 Asn Ala Lys Ile Asp Met Thr Glu Thr Ser Ser Ile Thr Asn Asn Met 500 505 510 Ala Thr His Leu Gln Val Leu Leu Asn Pro Arg Leu Asn Ala Asn Leu 515 520 525 Tyr Asp Phe 530 <210> 260 <211> 543 <212> PRT <213> Hydrastis canadensis <400> 260 Met Ala Asp Phe Thr Met Leu Leu Gln Trp Leu Leu Leu Thr Met Ala 1 5 10 15 Thr Leu Leu Phe Leu Asn Ala Val Tyr Lys Ser Ile Lys Ser Ser Lys 20 25 30 Asn Thr Ile Asn Asp Thr Ser Phe Lys Lys Gly Lys Lys Ala Pro Val 35 40 45 Ala Ala Gly Ala Arg Pro Phe Ile Gly His Leu His Met Leu Val Gly 50 55 60 Gly Lys Gln Leu Pro His Gln Ala Leu Gly Lys Leu Ala Asp Lys Tyr 65 70 75 80 Gly Pro Ala Phe Ile Ile Asn Ile Gly Pro Asn Pro Glu Leu Val Val 85 90 95 Ser Ser Trp Glu Leu Ala Lys Glu Cys Phe Thr Ile Asn Asp Arg Cys 100 105 110 Phe Thr Asp Arg Pro Ser Asn Lys Ala Met Lys Tyr Leu Thr Tyr Asp 115 120 125 Glu Ala Ser Val Gly Phe Ala Pro Tyr Gly Pro Leu Trp Val Glu Met 130 135 140 Arg Lys Ile Ala Lys Ser Asn Cys Ala Phe Gln Gln Arg Leu Gln Val 145 150 155 160 Gln Lys Arg Val Arg Val Leu Glu Ile Asp Leu Phe Phe Lys Glu Leu 165 170 175 His Glu Leu Trp Ser His Ala Gly Ala Gly Ala Thr Gly Thr Ser Val 180 185 190 Ile Pro Leu Ser Ile Asp Met Lys Lys Trp Phe Glu Glu Leu Thr Leu 195 200 205 Asn Val Ile Thr Arg Met Val Ser Gly Lys His Asn Tyr Ala Thr Lys 210 215 220 Ala Arg Lys Gly Asp Thr Glu Ala Lys Arg Phe Lys Arg Val Ile Ser 225 230 235 240 Glu Ala Ala His Phe Thr Gly Arg Leu Leu Leu Ser Asp Ile Phe Pro 245 250 255 Ser Leu Gly Phe Leu Asp Asn Met Gln Gly Arg Val Asn Ser Met Lys 260 265 270 Arg Thr Gly Lys Glu Leu Asp Ser Val Leu Ser Ser Trp Val Glu Glu 275 280 285 His Arg Gln Lys Arg Val Ser Gly Asn Lys Glu Gln Ser Pro Glu Asp 290 295 300 Asp Asp Val Glu Gln Asp Phe Ile Asp Leu Thr Leu Ser Met Met Glu 305 310 315 320 Glu Ile Gln Leu His Trp Thr Asp Ala Asp Thr Phe Ile Lys Ala Ile 325 330 335 Cys Val Gly Val Ile Leu Gly Gly Ser Asp Thr Thr Ser Val Gly Ile 340 345 350 Thr Trp Ile Leu Ser Leu Leu Met Asn His Pro Asp Val Leu Lys Lys 355 360 365 Ala Arg Glu Glu Leu Asp Gln Gln Val Gly Leu Glu Arg Lys Val Asp 370 375 380 Asp Ser Asp Leu Asn Asn Leu Ala Tyr Leu Arg Ala Ile Val Lys Glu 385 390 395 400 Ser Met Arg Leu Tyr Gln Val Gly Pro Leu Ile Glu Arg Lys Ala Lys 405 410 415 Gln Asp Cys Asn Ile Gly Gly Phe His Leu Lys Ala Gly Thr Arg Leu 420 425 430 Leu Val Asn Leu Ser Lys Val His Lys Asp Pro Thr Val Trp Ser Asp 435 440 445 Pro Asn Glu Phe Arg Pro Glu Arg Phe Phe Thr Thr His Thr Asn Ile 450 455 460 Asp Ile Lys Gly Gln Asn Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg 465 470 475 480 Arg Met Cys Pro Gly Tyr Leu Phe Ala Leu Ser Glu Ile Tyr Leu Val 485 490 495 Leu Ala Arg Leu Ile His Gly Phe Glu Leu Arg Thr Pro Asn Asp Ala 500 505 510 Lys Leu Asp Met Thr Glu Thr Ser Ser Val Thr Asn Tyr Arg Ala Thr 515 520 525 Pro Leu Gln Val Leu Leu Thr Pro Arg Leu Ser Ser Lys Leu Tyr 530 535 540 <210> 261 <211> 532 <212> PRT <213> Hydrastis canadensis <400> 261 Met Asp Tyr Phe Ser Met Pro Tyr Gln Trp Leu Leu Thr Ser Leu Ala 1 5 10 15 Thr Leu Ile Val Phe Val Phe Ile Trp Ser Ile Thr Ala Gly Lys Thr 20 25 30 Ser Thr Thr Ser Asn Lys Ala Lys Lys Ala Pro Pro Ile Ala Ala Gly 35 40 45 Ala Trp Pro Ile Ile Gly His Leu His Leu Ile Met Gly Gly Glu Leu 50 55 60 Pro His Arg Leu Leu Ser Asn Leu Ala Asp Lys Tyr Gly Pro Ile Phe 65 70 75 80 Met Leu Asn Tyr Gly Ser Gln Pro Gly Leu Val Val Ser Ser Leu Lys 85 90 95 Leu Ala Lys Glu Cys Phe Val His Asn Asp Arg Ala Val Phe Lys Arg 100 105 110 Pro Asn Ser Lys Ala Met Lys Tyr Phe Thr Tyr Asp Gln Ala Ser Phe 115 120 125 Gly Phe Ala Pro Tyr Gly Pro Leu Trp Val Glu Met Arg Lys Val Ser 130 135 140 Lys Ser Asn Leu Leu Ser Asn Gln Arg Leu Gln Leu Gln Arg Asn Gln 145 150 155 160 Arg Ala Ser Glu Val Asp Ala Phe Ile Lys Glu Leu Tyr Gln Leu Cys 165 170 175 Lys Lys Ser Asn Asn Gly Thr Leu Met Val Glu Met Asn Lys Trp Phe 180 185 190 Glu Glu Leu Thr Leu Asn Val Val Thr Arg Met Val Cys Gly Lys Lys 195 200 205 Asn Ile Gly Ala Lys Ala Arg His Gly Gly Asp Asn Glu Ala Lys Tyr 210 215 220 Tyr Lys Lys Val Ile Asp Glu Ala Thr Ile Phe Thr Ala Lys Leu Val 225 230 235 240 Ala Ser Asp Phe Phe Pro Ser Leu Gly Trp Val Asp Tyr Leu His Gly 245 250 255 Asp Glu Ser Ala Ile Lys Gln Thr Ala Lys Glu Leu Asp Ser Ile Ile 260 265 270 Gly Ser Trp Val Glu Glu His Arg Gln Lys Arg Leu Leu Ser Leu Asn 275 280 285 Lys Asp Asp Tyr Ala Glu Gln Asp Phe Ile Asp Ile Thr Leu Ser Met 290 295 300 Ile Asp Gln Thr Gln His Gln Gly Ile Asp Ala Asp Thr Phe Val Lys 305 310 315 320 Ser Met Cys Val Gly Met Ile Phe Gly Gly Ser Asp Ser Thr Ser Val 325 330 335 Ala Leu Asn Trp Ala Leu Ser Ile Leu Met Asn Asn Arg His Val Leu 340 345 350 Lys Arg Ala Arg Glu Glu Ile Asp Thr Leu Val Gly Lys Asp Arg Lys 355 360 365 Val Asn Asp Val Asp Val Thr Lys Leu Val Tyr Leu Lys Ala Ile Val 370 375 380 Lys Glu Ser Met Arg Leu Cys Leu Val Gly Pro Leu Leu Glu Arg Val 385 390 395 400 Thr Val Glu Asp Cys Glu Ile Gly Gly Phe Tyr Val Lys Ala Gly Ser 405 410 415 Arg Ile Val Val Asn Ile Trp Lys Leu Gln His Asp Pro Asp Leu Trp 420 425 430 Ser Asp Asp Val Met Glu Phe Arg Pro Glu Arg Phe Leu Thr Thr Asn 435 440 445 Ser Asn Val Glu Leu Arg Gly Gln His Phe Glu Leu Ile Pro Phe Gly 450 455 460 Ser Gly Ser Arg Met Cys Pro Gly Val Thr Phe Ala Leu Glu Leu Met 465 470 475 480 His Leu Thr Leu Ala Arg Leu Ile His Gly Phe Glu Leu Gly Thr Pro 485 490 495 Met Asp Ala Lys Val Asp Met Thr Glu Thr Ser Ser Val Thr Asn Tyr 500 505 510 Lys Ala Thr Pro Leu Glu Ile Leu Leu Thr Pro Arg Leu His Pro Lys 515 520 525 Leu Tyr Asp Phe 530 <210> 262 <211> 534 <212> PRT <213> Hydrastis canadensis <400> 262 Met Phe Ile Ser Ile Thr Gln Gln Ser Met Asp Ser Phe His Gln Tyr 1 5 10 15 Leu Ala Thr Ile Ile Phe Gly Val Phe Ser Phe Val Leu Phe Leu Ile 20 25 30 Tyr Tyr Leu Pro Leu Lys Arg Ser Arg Ser Asp Lys Lys Arg Ala Ala 35 40 45 Pro Lys Pro Val Gly Ala Trp Pro Ile Ile Gly His Leu Pro Met Leu 50 55 60 Ser Arg His Gln Pro Pro His Ile Thr Leu Gly Asn Ile Ala Asp Lys 65 70 75 80 Tyr Gly Pro Ala Phe Thr Leu Gln Leu Gly Val His Arg Ala Leu Val 85 90 95 Val Ser Ser Ser Glu Val Ala Lys Glu Cys Phe Thr Thr Asn Asp Lys 100 105 110 Ala Leu Ala Ser Arg Pro Ser Ser Val Ala Leu Lys Leu Met Gly Tyr 115 120 125 Asn Asn Ala Met Phe Gly Phe Gly Pro Tyr Gly Ser Tyr Trp Arg Gln 130 135 140 Met Arg Lys Ile Val Val Leu Glu Leu Leu Ser Asn His Arg Leu Gln 145 150 155 160 Leu Leu Lys His Val Arg Ile Ser Glu Val Ser Thr Ser Leu Lys Glu 165 170 175 Leu Tyr Gln Val Trp Ala Ser Cys Thr Asn Lys Asn Asp Lys Gly Gln 180 185 190 Val Leu Val Asp Met Gln Gln Trp Phe Gly Asp Leu Thr Leu Asn Val 195 200 205 Ser Val Arg Met Ile Ala Gly Lys Arg Tyr Phe Gly Ala Ser Ala Ala 210 215 220 Cys Asp Glu Asp Glu Ala Arg Arg Phe Gln Lys Ala Ile Lys Asp Phe 225 230 235 240 Leu His Leu Val Gly Leu Phe Val Val Ser Asp Ala Leu Pro Phe Leu 245 250 255 Glu Trp Leu Asp Ile Gln Gly His Gln Lys Ala Met Lys Arg Thr Phe 260 265 270 Lys Glu Leu Asp Arg Ile Leu Gly Lys Trp Val Glu Glu His Arg Arg 275 280 285 Asn Lys Leu Asp Gly Gly Thr Asn Val Gly Arg Asp Phe Met Asp Val 290 295 300 Met Ser Ser Ile Leu Asp Asp Ala Asn Ile Ser Asp Tyr Asp Ala Asp 305 310 315 320 Thr Ile Asn Lys Ala Thr Cys Leu Ser Leu Ile Leu Gly Gly Thr Asp 325 330 335 Thr Thr Met Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Met Asn Asn 340 345 350 Gln His Ile Leu Lys Lys Ala Gln Asp Glu Ile Asp Ile Tyr Val Gly 355 360 365 Lys Asp Lys Asn Val Asp Glu Ser Asp Ile Glu Lys Leu Val Tyr Leu 370 375 380 Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Ala Gly Pro Leu 385 390 395 400 Ser Gly Pro His Glu Ala Ile Glu Asp Cys Thr Val Ala Gly Tyr His 405 410 415 Val Pro Arg Gly Thr Arg Leu Ile Thr Asn Leu Trp Lys Ile Gln Arg 420 425 430 Asp Pro Arg Ile Trp Ser Ser Pro Cys Glu Phe Gln Pro Glu Arg Phe 435 440 445 Leu Thr Thr Gln Ala Asn Val Asp Val Arg Gly Gln His Phe Glu Phe 450 455 460 Leu Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Ala Thr Ser Phe Ala 465 470 475 480 Leu Gln Val Val His Leu Ala Leu Ala Arg Val Leu Gln Gly Phe Glu 485 490 495 Phe Glu Thr Gln Ser Asn Ala Pro Val Asp Met Thr Glu Ser Ala Gly 500 505 510 Leu Thr Asn Val Lys Ala Thr Pro Leu Glu Val Leu Ile Thr Pro Arg 515 520 525 Leu Pro Leu Asn Leu Tyr 530 <210> 263 <211> 529 <212> PRT <213> Hydrastis canadensis <400> 263 Met Glu Ser Leu His Gln Tyr Leu Ala Ala Val Met Ser Cys Ile Phe 1 5 10 15 Ala Leu Leu Leu Phe Leu Tyr Tyr Phe Pro Trp Lys Ile Arg Arg Ser 20 25 30 Ser Ser Asp Asn Asn Met Arg Ser Ala Pro Glu Ala Ala Gly Ala Trp 35 40 45 Pro Ile Ile Gly His Leu Pro Met Leu Gly Gly His Gln Leu Pro His 50 55 60 Ile Thr Leu Gly Asn Leu Ala Asp Arg Tyr Gly Pro Ala Phe Thr Ile 65 70 75 80 Arg Leu Gly Val Cys Arg Ala Leu Val Val Ser Ser Ser Glu Val Ala 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Lys Ala Phe Ala Thr Arg Pro Ser 100 105 110 Ser Val Ala Val Lys Leu Met Gly Tyr Asn Tyr Ala Leu Phe Gly Leu 115 120 125 Ala Pro Tyr Thr Ser Tyr Trp Arg Glu Val Arg Lys Ile Val Ile Leu 130 135 140 Glu Leu Leu Ser Asn Arg Arg Leu Glu Leu Leu Lys His Val Arg Ile 145 150 155 160 Ser Glu Val Asn Thr Ser Ile Lys Glu Leu Phe Gln Val Trp Ala Ser 165 170 175 Ser Asn Asn Lys Asn Glu Lys Gly Gln Val Leu Val Glu Met Gln Arg 180 185 190 Trp Phe Gly Asp Leu Thr Ile Asn Val Ala Val Arg Met Val Ala Gly 195 200 205 Lys Arg Tyr Phe Gly Ala Ser Val Asn Thr Cys Asp Asp Glu Glu Glu 210 215 220 Ala Arg Arg Phe Gln Lys Ala Ile Lys Asn Phe Phe His Leu Val Gly 225 230 235 240 Leu Tyr Val Leu Ser Asp Ser Leu Pro Phe Leu Glu Trp Leu Asp Phe 245 250 255 Glu Gly His Gln Lys Ala Met Lys Arg Thr Phe Lys Asp Val Asp Cys 260 265 270 Ile Leu Gln Arg Trp Leu Glu Glu His Arg Arg Asp Lys Glu Asn Gly 275 280 285 Ala Met Lys Glu Glu Arg Asp Phe Met Asp Val Met Leu Ser Ile Leu 290 295 300 Lys Asp Ala Asn Leu Phe Gly Tyr Glu Ala Asp Thr Val Asn Lys Ala 305 310 315 320 Thr Ser Leu Asn Ile Ile Leu Gly Ala Thr Asp Thr Thr Met Val Thr 325 330 335 Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His Ile Leu Lys 340 345 350 Lys Ala Glu Asp Glu Ile Asp Ile His Val Gly Lys Asp Lys Ala Val 355 360 365 Asp Glu Ser Asp Ile Glu Lys Leu Val Tyr Leu Gln Ala Ile Val Lys 370 375 380 Glu Thr Leu Arg Leu Tyr Pro Val Ala Pro Leu Leu Ala Ala His Glu 385 390 395 400 Ala Ile Glu Asp Cys Ile Val Ala Gly Tyr His Val Pro Arg Gly Thr 405 410 415 Arg Leu Ile Pro Asn Ile Trp Lys Ile Gln Arg Asp Pro Arg Ile Trp 420 425 430 Ser Ser Pro Cys Asp Phe Gln Pro Glu Arg Phe Leu Thr Thr Gln Ala 435 440 445 Asn Val Asp Val Arg Gly Gln His Phe Glu Leu Phe Pro Phe Gly Ser 450 455 460 Gly Arg Arg Met Cys Pro Gly Ile Ser Phe Gly Leu Gln Val Val His 465 470 475 480 Leu Ala Leu Ala Arg Val Leu Gln Gly Phe Glu Phe Asp Thr Pro Ser 485 490 495 Asn Lys Ala Ile Asp Met Thr Glu Ser Ala Gly Leu Thr Asn Leu Lys 500 505 510 Ala Thr Pro Leu Gln Val Leu Ile Thr Pro Cys Leu Pro Leu Asn Leu 515 520 525 Tyr <210> 264 <211> 530 <212> PRT <213> Hydrastis canadensis <400> 264 Met Asp Ser Ile Leu Leu Pro Thr Pro Leu Met Val Ser Leu Phe Ala 1 5 10 15 Leu Leu Leu Cys Leu Tyr Tyr Leu Ile Ile Ser Arg Pro Arg Ser Ser 20 25 30 His Asn Thr Ser Thr Lys Glu Ala Pro Glu Ala Ala Gly Ala Leu Pro 35 40 45 Ile Ile Gly His Leu His Leu Leu Gly Gly Arg Ile Leu Pro His Ile 50 55 60 Thr Leu Gly Ala Met Ala Asp Lys His Gly Pro Ala Phe Met Ile Arg 65 70 75 80 Ile Gly Val His Arg Ala Leu Val Val Ser Ser Ser Glu Val Ala Lys 85 90 95 Glu Cys Phe Thr Thr Asn Asp Lys Ala Phe Ala Ser Arg Pro Lys His 100 105 110 Thr Ala Ala Glu Leu Met Gly Tyr Asn Tyr Ala Met Phe Gly Phe Ala 115 120 125 Pro Tyr Gly Pro Phe Trp Ser Glu Met Arg Lys Ile Ile Met Ala Glu 130 135 140 Leu Leu Ser Asn Arg Arg Leu Glu Leu Leu Lys Tyr Ile Arg Asp Phe 145 150 155 160 Glu Leu Lys Ala Ser Ile Lys Glu Leu Tyr Met Thr Trp Glu Asn His 165 170 175 Ser Val Thr Asn Lys Gly Gln Val Val Val Glu Met Lys Lys Trp Phe 180 185 190 Gly Asp Leu Thr Leu Asn Val Ile Leu Arg Met Ile Ala Gly Lys Arg 195 200 205 Tyr Phe Gly Ser Asn Ser Thr Cys Asp Glu Ser Glu Ala Lys Ile Cys 210 215 220 Gln Lys Gly Met Arg Asp Phe Phe Arg Leu Leu Gly Glu Phe Leu Val 225 230 235 240 Glu Asp Ala Ile Pro Tyr Leu Gly Trp Leu Asp Leu Gln Gly Phe Lys 245 250 255 Lys Glu Met Lys Asn Thr Ala Lys Glu Leu Asp Ile Leu Leu Gln Gly 260 265 270 Trp Leu Glu Glu His Lys Lys Lys Arg Glu Phe Ser Lys Asp Val Lys 275 280 285 Glu Glu Gln Asp Phe Met Asp Val Met Met Thr Ile Leu Glu Asp Ala 290 295 300 Asn Phe Ser Asp Phe Asp Ala Asp Thr Ile Asn Lys Ala Thr Cys Leu 305 310 315 320 Thr Ile Ile Ser Gly Gly Ser Asp Thr Thr Met Leu Thr Leu Thr Trp 325 330 335 Ala Leu Ser Leu Leu Leu Asn Asn Gln His Val Leu Lys Lys Ala Gln 340 345 350 Asp Glu Leu Asp Thr His Val Gly Arg Asp Arg Arg Val Asp Glu Ser 355 360 365 Asp Ile Lys Asn Leu Val Tyr Leu Asn Ala Ile Val Lys Glu Thr Leu 370 375 380 Arg Leu Tyr Pro Ala Ser Pro Leu Leu Gly Ile Arg Val Ser Thr Glu 385 390 395 400 Asp Cys Thr Val Ala Gly Tyr His Val Pro Ser Gly Thr Arg Leu Met 405 410 415 Val Asn Ala Trp Lys Ile Gln Arg Asp Pro Leu Val Trp Ser Asp Pro 420 425 430 Phe Glu Phe Arg Pro Glu Arg Phe Leu Thr Thr His Val Asn Val Asp 435 440 445 Val Lys Gly Gln Asn Phe Asn Leu Ile Pro Phe Ala Ser Gly Arg Arg 450 455 460 Val Cys Pro Gly Val Ala Phe Ala Leu Gln Met Leu Pro Leu Val Leu 465 470 475 480 Ala His Leu Leu His Gly Phe Lys Leu Met Thr Gln Leu Gly Gly Pro 485 490 495 Val Asp Met Thr Glu Ser Thr Gly Leu Thr Asn Ile Lys Ala Thr Pro 500 505 510 Leu Glu Val Val Ile Ser Pro Arg Leu Arg Pro Glu Leu Tyr Glu Met 515 520 525 Tyr Thr 530 <210> 265 <211> 525 <212> PRT <213> Hydrastis canadensis <400> 265 Met Glu Ser Leu His Gln Tyr Leu Ala Thr Ile Leu Ser Cys Ile Phe 1 5 10 15 Ala Phe Leu Leu Phe Leu Tyr Tyr Phe Pro Trp Lys Gly Arg Arg Ser 20 25 30 Phe Asp Asn Asn Leu Arg Thr Ala Pro Glu Ala Ala Gly Ala Trp Pro 35 40 45 Ile Ile Gly His Leu Pro Met Leu Asn Gln Arg Asp Leu Pro His Val 50 55 60 Thr Leu Gly Asn Leu Ala Asp Arg Tyr Gly Pro Ala Phe Thr Ile Arg 65 70 75 80 Leu Gly Val Arg Arg Ala Leu Val Val Ser Ser Ser Glu Val Ala Lys 85 90 95 Glu Cys Phe Thr Thr Asn Asp Lys Ala Phe Ala Thr Arg Pro Ser Ser 100 105 110 Val Ala Val Lys Leu Met Ala Tyr Asn Tyr Ala Val Phe Gly Phe Gly 115 120 125 Pro Tyr Gly Ser Tyr Trp Arg Glu Met Arg Lys Ile Val Ile Leu Glu 130 135 140 Leu Leu Ser Asn Arg Arg Leu Glu Leu Leu Lys His Val Arg Ile Ser 145 150 155 160 Glu Val Asn Thr Ser Ile Lys Glu Leu Tyr Gln Val Trp Ala Ser Asn 165 170 175 Asn Lys Asn Glu Lys Gly Gln Val Leu Val Glu Met Gln Arg Trp Phe 180 185 190 Gly Asp Leu Thr Met Asn Val Val Val Arg Met Val Ala Gly Lys Arg 195 200 205 Tyr Phe Gly Ala Ser Val Thr Cys Asp Glu Glu Glu Ala Arg Arg Phe 210 215 220 Gln Lys Ala Ile Arg Asp Phe Ser His Leu Ala Gly Leu Tyr Val Leu 225 230 235 240 Ser Asp Ala Leu Pro Asn Ile Glu Trp Leu Asp Phe Glu Gly His His 245 250 255 Lys Ala Met Lys Lys Thr Phe Lys Asp Leu Asp Cys Ile Leu Gln Arg 260 265 270 Trp Leu Glu Glu His Arg Arg Asp Arg Glu Asn Gly Ala Thr Lys Gly 275 280 285 Glu Arg Asp Phe Met Asp Val Met Leu Ser Ile Leu Lys Asp Ala Asn 290 295 300 Pro Phe Asp Phe Glu Ala Asp Thr Val Asn Lys Ala Thr Ser Leu Asn 305 310 315 320 Met Val Leu Gly Gly Thr Glu Thr Thr Thr Val Thr Leu Thr Trp Ala 325 330 335 Leu Ser Leu Leu Leu Asn Asn Gln His Ile Leu Lys Lys Ala Gln Asp 340 345 350 Glu Ile Asn Ile Arg Val Gly Lys Asp Lys Pro Val Asp Glu Ser Asp 355 360 365 Ile Glu Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 370 375 380 Leu Tyr Pro Val Leu Pro Leu Ser Val Pro His Glu Ala Ile Glu Asp 385 390 395 400 Cys Thr Ile Ala Gly Tyr His Val Pro Arg Gly Thr Arg Leu Ile Thr 405 410 415 Asn Leu Trp Lys Ile Gln Arg Asp Pro His Ile Trp Ser Ser Pro Cys 420 425 430 Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr Gln Ala Asn Val Asp Val 435 440 445 Arg Gly Gln His Phe Glu Leu Phe Pro Phe Gly Ser Gly Arg Arg Met 450 455 460 Cys Pro Gly Ile Ser Phe Gly Leu Gln Val Val His Leu Ala Leu Ala 465 470 475 480 Arg Val Leu Gln Gly Phe Glu Phe Glu Thr Pro Ser Asn Val Pro Met 485 490 495 Asp Met Thr Glu Ser Ala Gly Leu Thr Asn Phe Lys Thr Thr Pro Leu 500 505 510 Glu Val Leu Ile Thr Pro Cys Leu Pro Leu Asp Gln Tyr 515 520 525 <210> 266 <211> 525 <212> PRT <213> Hydrastis canadensis <400> 266 Met Asp Ser Leu Leu Gln Leu Gln Ile Ile Gly Ala Leu Ala Ala Leu 1 5 10 15 Ile Phe Thr Tyr Lys Leu Leu Lys Val Ile Cys Arg Ser Pro Met Thr 20 25 30 Asp Gly Met Glu Ala Pro Glu Pro Pro Gly Ala Trp Pro Ile Ile Gly 35 40 45 His Leu His Leu Leu Gly Gly Gln Asp Pro Ile Ala Arg Thr Leu Gly 50 55 60 Val Met Thr Asp Lys Tyr Gly Pro Ile Leu Lys Leu Arg Leu Gly Val 65 70 75 80 His Thr Gly Leu Val Val Ser Asn Trp Glu Leu Ala Lys Glu Cys Phe 85 90 95 Thr Thr Asn Asp Arg Val Leu Ala Ser Arg Pro Met Gly Ala Ala Gly 100 105 110 Lys Tyr Leu Gly Tyr Asn Tyr Ala Ile Phe Gly Leu Ala Pro His Gly 115 120 125 Pro Tyr Trp Ser Glu Val Arg Lys Ile Val Leu Arg Glu Leu Leu Ser 130 135 140 Asn Gln Ser Leu Glu Lys Leu Lys His Val Arg Ile Ser Glu Ile Asn 145 150 155 160 Thr Cys Leu Lys Asn Leu Phe Ser Leu Asn Asn Gly Asn Thr Pro Ile 165 170 175 Lys Val Asp Met Lys Gln Trp Phe Glu Arg Pro Met Phe Asn Val Val 180 185 190 Thr Met Met Ile Ala Gly Lys Arg Tyr Phe Ser Met Glu Asn Asp Asn 195 200 205 Glu Ala Met Asn Phe Arg Lys Val Ala Thr Glu Phe Met Tyr Leu Thr 210 215 220 Gly Val Phe Val Val Ser Asp Ala Leu Pro Tyr Leu Glu Trp Leu Asp 225 230 235 240 Leu Gln Gly His Val Ser Ala Met Lys Arg Thr Ala Lys Glu Leu Asp 245 250 255 Ile His Val Gly Lys Trp Leu Glu Glu His Arg Arg Ala Lys Leu Leu 260 265 270 Gly Glu Thr Lys Asn Glu Asp Asp Phe Val Asp Val Leu Leu Thr Ile 275 280 285 Leu Pro Glu Asp Leu Lys Asp Asn Gln Thr Tyr Ile His Asp Arg Asp 290 295 300 Thr Ile Ile Lys Ala Thr Ala Leu Ala Leu Phe Leu Ala Ala Ser Asp 305 310 315 320 Thr Thr Ala Ile Thr Leu Thr Trp Ala Leu Ser Leu Ile Leu Asn Asn 325 330 335 Pro Asp Val Leu Lys Arg Ala Gln Asp Glu Leu Asp Lys His Val Gly 340 345 350 Lys Glu Lys Leu Val Lys Glu Ser Asp Ile Ile Asn Leu Val Tyr Leu 355 360 365 Gln Ala Ile Ile Lys Glu Thr Leu Arg Leu Tyr Pro Ala Ala Pro Leu 370 375 380 Leu Leu Pro His Glu Ala Met Glu Asp Cys Thr Val Gly Gly Tyr His 385 390 395 400 Val Pro Lys Gly Thr Arg Ile Phe Val Asn Ile Trp Lys Leu Gln Arg 405 410 415 Asp Pro Arg Val Trp Phe Asp Pro Asn Glu Phe Arg Pro Glu Arg Phe 420 425 430 Leu Thr Thr His Ala Asn Val Asp Phe Lys Gly Gln His Phe Glu Tyr 435 440 445 Ile Pro Phe Ser Ser Gly Arg Arg Val Cys Pro Gly Ile Thr Phe Ser 450 455 460 Thr Gln Ile Met His Leu Thr Leu Ala His Leu Leu His Glu Phe Asn 465 470 475 480 Ile Val Thr Pro Thr Lys Ser Asn Ala Gly Val Asp Met Thr Glu Ser 485 490 495 Leu Gly Ile Thr Met Pro Lys Ala Thr Pro Leu Glu Val Leu Leu Thr 500 505 510 Pro Arg Leu Pro Ser Asn Leu Tyr Asn Gln Tyr Arg Asp 515 520 525 <210> 267 <211> 521 <212> PRT <213> Jeffersonia diphylla <400> 267 Met Asp Ser Leu Gln Leu Ile Ala Ser Val Val Cys Gly Leu Phe Ala 1 5 10 15 Phe Leu Arg Ile Tyr Tyr Leu Leu Lys Lys Leu Ser Ser Asn Lys Glu 20 25 30 Ala Pro Gln Leu Gly Gly Ala Trp Pro Ile Ile Gly His Leu His Leu 35 40 45 Leu Gly Arg Val Glu Leu Pro His Ile Phe Phe Ser Ala Met Ala Asp 50 55 60 Lys Tyr Gly Pro Ala Phe Met Ile Gln Leu Gly Met Arg Arg Ala Leu 65 70 75 80 Val Val Ser Ser Ala Glu Ile Ala Lys Glu Cys Leu Thr Thr Asn Asp 85 90 95 Lys Ala Leu Ala Thr Arg Pro Ser Ser Ile Ala Ala Glu Ile Met Gly 100 105 110 Tyr Asp Tyr Ala Met Phe Gly Leu Gly Pro Tyr Gly Asp Tyr Trp Arg 115 120 125 Glu Val Arg Lys Ile Val Val Leu Glu Phe Leu Ser Asn Arg Arg Leu 130 135 140 Glu Leu Leu Lys His Val Arg Val Ser Glu Ile Ser Leu Ser Leu Lys 145 150 155 160 Glu Leu Tyr Gln Ser Trp Glu Lys Gln Asn Lys Ala Asp Glu Pro Val 165 170 175 Leu Val His Leu Asp Gln Trp Phe Gly Asp Met Thr Leu Asn Leu Ser 180 185 190 Val Arg Met Val Ala Gly Lys Arg Tyr Phe Gly Ala Thr Ala Ala Cys 195 200 205 Gly Glu Asp Glu Ser Arg Lys Val Gln Lys Ala Thr Arg Glu Phe Asp 210 215 220 Arg Leu Leu Gly Leu Phe Val Val Ser Asp Tyr Leu Pro Phe Leu Arg 225 230 235 240 Trp Leu Asp Leu Glu Gly His Gln Lys Leu Met Lys Ser Ile Gly Arg 245 250 255 Glu Leu Asp Ser Ile Leu Gln Gly Trp Leu Asp Glu His Lys Thr Arg 260 265 270 Arg Asn Ser Gly Gly Gly Ala Lys Gly Asp Gln Asp Phe Ile Asp Val 275 280 285 Met Leu Ser Val Leu Glu Asp Gly Asn Leu Ser Asn Tyr Asp Ala Asp 290 295 300 Lys Leu Asn Lys Ala Thr Ser Leu Thr Met Ile Val Ala Gly Ser Glu 305 310 315 320 Thr Thr Met Val Thr Leu Thr Trp Ala Val Cys Leu Leu Leu Asn Asn 325 330 335 Pro Glu Val Leu Asn Lys Ala Gln Asp Glu Leu Asp Met His Ile Gly 340 345 350 Lys Asp Arg Gln Val Glu Glu Ser Asp Ile Lys Asn Leu Ala Tyr Leu 355 360 365 Gln Ala Val Ile Lys Glu Thr Met Arg Leu Tyr Pro Val Ala Pro Leu 370 375 380 Leu Ile Pro His Glu Ala Met Glu Asp Cys Thr Ile Ala Gly Tyr His 385 390 395 400 Val Arg Ala Gly Thr Arg Val Ile Leu Asn Gly Trp Lys Leu Gln Arg 405 410 415 Asp Pro Phe Ile Trp Ser Asp Pro Cys Glu Phe Gln Pro Glu Arg Phe 420 425 430 Leu Asn Asn Asp Val Asp Val Arg Gly Arg His Phe Glu Leu Leu Pro 435 440 445 Phe Gly Ser Gly Arg Arg Ala Cys Pro Gly Ile Ser Leu Ala Leu Asn 450 455 460 Val Val Ser Leu Thr Leu Ala Arg Leu Leu His Glu Phe Lys Phe Ser 465 470 475 480 Asn Pro Ser Glu Gly Lys Val Asp Met Ser Glu Ser Ala Gly Val Val 485 490 495 Val Ala Lys Ala Thr Pro Leu Glu Val Leu Ile Ile Pro Arg Leu Pro 500 505 510 Ser Lys Phe Tyr Glu Tyr Tyr Lys Leu 515 520 <210> 268 <211> 518 <212> PRT <213> Hydrastis canadensis <400> 268 Met Phe Ser Leu Gln Leu Leu Ser Thr Pro Leu Phe Gly Phe Phe Thr 1 5 10 15 Leu Leu Ala Ile Tyr Tyr Leu Leu Trp Thr Lys Ser Asn Lys Thr Lys 20 25 30 Glu Ala Pro Gln Pro Ala Gly Ala Trp Pro Ile Ile Gly His Leu His 35 40 45 Leu Leu Ala Arg Ser Glu Leu Pro His Ile Thr Leu Gly Ala Met Ala 50 55 60 Asp Lys Tyr Gly Pro Ala Phe Met Ile Arg Leu Gly Thr Arg Gln Ala 65 70 75 80 Ile Val Val Ser Asn Ser Glu Ile Ala Lys Glu Cys Leu Thr Ile Lys 85 90 95 Asp Arg Ile Phe Ala Thr Arg Pro Ser Leu Val Ala Phe Lys Leu Met 100 105 110 Gly Tyr Asn Ser Ala Met Val Gly Leu Ser Pro Tyr Gly Pro Tyr Trp 115 120 125 Arg Glu Leu Arg Lys Ile Ile Ala Leu Lys Val Leu Ser Gln Arg Arg 130 135 140 Leu Glu Ser Leu Lys Asp Val Trp Asp Ser Glu Ile Ser Ser Ser Leu 145 150 155 160 Asn His Leu His Gln Ile Trp Ser His Gln Thr Glu Pro Asn Gly Lys 165 170 175 Ile Leu Val Glu Met Asp Gln Trp Phe Gly Asp Leu Thr Leu Asn Val 180 185 190 Ala Val Lys Met Val Ala Gly Lys Arg Phe Phe Ser Ala Asp Ala Ala 195 200 205 Cys Asp Glu Asn Glu Ser Arg Arg Cys Gln Lys Ala Ile Arg Glu Phe 210 215 220 Phe Arg Leu Leu Gly Gln Phe Val Val Ser Asp Ser Leu Pro Phe Leu 225 230 235 240 Gly Trp Leu Asp Leu Glu Gly Tyr Gln Lys Glu Met Lys Ser Thr Ala 245 250 255 Lys Glu Val Asp Ser Ile Leu Gln Lys Trp Leu Asp Glu His Lys Gln 260 265 270 Lys Arg Gln Ser Ala Gly Ala Asp Arg Asp Gln Asp Phe Met Asp Val 275 280 285 Met Leu Ser Leu Leu Glu Asp Ala Ser Leu Ser Gln Tyr Asp Thr Asp 290 295 300 Thr Ile Asn Lys Ala Thr Cys Phe Ser Met Ile Val Gly Gly Ser Asp 305 310 315 320 Thr Thr Lys Ile Thr Leu Thr Trp Ala Leu Thr Leu Leu Leu Asn Asn 325 330 335 Pro Asp Val Met Lys Lys Ser Gln Asp Glu Leu Asp Ile Gln Val Gly 340 345 350 Lys Asp Arg His Val Glu Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu 355 360 365 Glu Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Ala Ala Pro Leu 370 375 380 Leu Ala Pro His Glu Ala Thr Glu Asp Cys Ile Val Ala Gly Tyr Asn 385 390 395 400 Val Arg Ala Gly Thr Arg Leu Ile Val Asn Ala Trp Lys Ile Gln Arg 405 410 415 Asp Pro Leu Ile Trp Pro Asn Pro Ser Glu Phe Leu Pro Glu Arg Tyr 420 425 430 Leu Asn Lys Asp Val Asp Val Lys Gly Gln His Phe Glu Leu Ile Pro 435 440 445 Phe Gly Ser Gly Arg Arg Ala Cys Pro Gly Ile Thr Phe Gly Leu Gln 450 455 460 Val Val Ser Leu Thr Leu Ala Arg Leu Leu His Glu Phe Glu Ile Ser 465 470 475 480 Ile Ala Ser Gly Ile Lys Val Asp Met Thr Glu Ser Gly Gly Leu Val 485 490 495 Thr Ala Lys Ala Thr Pro Leu Lys Ala Leu Ile Ala Pro Arg Phe Leu 500 505 510 Tyr Ser His His His Ile 515 <210> 269 <211> 525 <212> PRT <213> Mahonia aquifolium <400> 269 Met Asn Ser Leu Gln Phe Leu Ala Thr Pro Leu Leu Thr Leu Ile Ala 1 5 10 15 Leu Leu Cys Val Tyr Cys Ile Val Trp Arg Lys Ser Phe Ser Arg Asn 20 25 30 Gly Ser Lys Ile Lys Gln Ala Pro Trp Ala Gly Gly Ala Trp Pro Ile 35 40 45 Met Gly His Leu His Leu Leu Ala Trp Gly Asp Leu Pro His Ile Thr 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Met Met Gln Leu 65 70 75 80 Gly Met Arg Gln Ala Ile Val Val Ser Ser Ala Glu Ile Ala Arg Glu 85 90 95 Cys Phe Thr Thr Asn Asp Arg Ile Leu Ala Thr Arg Pro Ser Ser Ile 100 105 110 Ala Val Lys Leu Met Ala Tyr Asn Tyr Ala Met Phe Ala Phe Ala Pro 115 120 125 Tyr Gly Pro Asn Trp Arg Glu Ile Arg Lys Thr Val Met Leu Glu Leu 130 135 140 Leu Ser Thr Pro Arg Leu Glu Ser Leu Lys His Val Trp Glu Asp Glu 145 150 155 160 Ile Ser Thr Ser Val Lys Glu Leu Tyr Glu Leu Cys Ala Ser Lys Thr 165 170 175 Lys Thr His Gly His Val Ser Val Asp Met Lys Gln Arg Phe Gly Asp 180 185 190 Leu Thr Leu Asn Val Leu Val Lys Leu Val Asn Gly Lys Arg Tyr Phe 195 200 205 Gly Ala Asn Thr Asp Tyr Thr Glu His Glu Ser Arg Arg Cys Gln Lys 210 215 220 Ala Val Arg Asp Phe Phe Ser Leu Met Gly Leu Phe Val Val Ser Asp 225 230 235 240 Phe Leu Pro Phe Leu Glu Arg Leu Asp Leu Gln Gly Tyr Glu Lys Glu 245 250 255 Met Lys Arg Val Ala Arg Glu Val Asp Gly Ile Val Gln Gly Trp Leu 260 265 270 Asp Glu His Lys Arg Lys Arg Glu Ser Ser Glu Thr Thr His Asp Gln 275 280 285 Asp Phe Met Asp Val Met Leu Ser Ile Leu Lys Asp Ser Lys Phe Ser 290 295 300 Asn Ser Asp Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr Cys Phe Asn 305 310 315 320 Met Ile Leu Gly Gly Ser Asp Thr Thr Met Ser Thr Met Thr Trp Ala 325 330 335 Leu Ser Ala Leu Leu Asn Asn Pro His Met Leu Lys Lys Ala Gln Asp 340 345 350 Glu Leu Glu Phe His Val Gly Lys Gly Arg Gln Val Glu Glu Ser Asp 355 360 365 Ile Lys Lys Leu Thr Tyr Leu Gln Ala Ile Ile Lys Glu Thr Leu Arg 370 375 380 Leu Tyr Pro Ser Ala Pro Leu Leu Ala Pro Arg Glu Ala Thr Glu Asp 385 390 395 400 Cys Lys Ile Ala Gly Tyr His Val Pro Ala Gly Thr Arg Leu Ile Val 405 410 415 Asn Val Trp Lys Ile His Arg Asp Pro Phe Val Trp Pro Asn Pro Asn 420 425 430 Glu Phe Gln Pro Glu Arg Phe Leu Asn Lys Asp Val Asp Val Lys Gly 435 440 445 Gln His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg Arg Ala Cys Pro 450 455 460 Gly Val Ser Leu Gly Leu Gln Val Val Ser Leu Gly Leu Ala Arg Leu 465 470 475 480 Leu His Gly Phe Glu Leu Ser Ile Pro Ser Gly Ser Lys Val Asp Met 485 490 495 Thr Glu Thr Ala Ser Leu Val Thr Phe Lys Ala Thr Pro Leu Glu Val 500 505 510 Phe Ile Thr Pro Arg Leu Ser Pro His Met Tyr Val Glu 515 520 525 <210> 270 <211> 502 <212> PRT <213> Mahonia aquifolium <400> 270 Tyr Ala Trp Lys Arg Lys Asn His Thr Lys Asp Ser Lys Ile Lys Glu 1 5 10 15 Pro Pro Gln Pro Ala Gly Ala Trp Pro Ile Ile Gly His Leu His Leu 20 25 30 Ile Ser Arg Gly Gly Leu Pro His Ile Asn Met Gly Ala Met Ala Asp 35 40 45 Lys Tyr Gly Pro Val Phe Met Ile Arg Leu Gly Val Asn Arg Ala Val 50 55 60 Ile Val Ser Asn Ser Glu Ile Val Lys Glu Cys Phe Thr Thr Asn Asp 65 70 75 80 Lys Phe Leu Leu Asn Arg Pro Val Gly Leu Ala Leu Asn Leu Met Ser 85 90 95 Tyr Asn Asn Ala Met Phe Gly Phe Ser Arg Tyr Gly Pro Tyr Trp Arg 100 105 110 Glu Ile His Lys Ile Val Met Leu Glu Leu Leu Ser Ser Ser Arg Leu 115 120 125 Glu Ser Leu Lys His Val Trp Asp Ser Glu Ile Leu Thr Ser Ile Glu 130 135 140 Glu Leu Tyr Gln Leu Cys Gln Thr Gln Asn Lys Ala His Glu Gly Pro 145 150 155 160 Val Leu Val Glu Met Lys Asp Trp Phe Ala Asp Met Ala Leu Asn Val 165 170 175 Ser Phe Arg Met Val Ala Gly Lys Arg Tyr Phe Gly Ala Ser Ala Gly 180 185 190 Gly Asn Lys Asp Glu Ala Arg Arg Cys Gln Lys Thr Met Arg Asp Phe 195 200 205 Phe Arg Leu Val Gly Leu Phe Ile Val Ser Asp Ala Leu Pro Phe Leu 210 215 220 Arg Trp Leu Asp Leu Gly Gly His Gln Lys Glu Met Lys Arg Thr Phe 225 230 235 240 Glu Asp Leu Asp Tyr Val Leu Gln Gly Trp Leu Asp Glu His Lys Leu 245 250 255 Asn Arg Lys Ser Gly Gly Asn Gly Asp Gln Asp Phe Met Asp Val Met 260 265 270 Leu Ser Thr Leu Tyr Asp Ser Lys Phe Thr Asp Tyr Asp Val Asp Thr 275 280 285 Ile Asn Lys Ala Thr Cys Phe Gln Leu Ile Leu Gly Gly Thr Asp Thr 290 295 300 Thr Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Met Leu Asn His Pro 305 310 315 320 His Val Leu Lys Lys Ala Gln Asp Glu Leu Asp Ile Gln Val Gly Lys 325 330 335 His Arg Gln Val Glu Glu Ser Asp Ile Lys Asn Leu Lys Tyr Leu Glu 340 345 350 Ala Val Ile Lys Glu Thr Leu Arg Met Arg Pro Val Gly Pro Leu Leu 355 360 365 Gly Pro Arg Glu Ala Ile Glu Asp Cys Thr Ile Gly Gly Tyr Arg Val 370 375 380 Arg Ala Gly Thr Arg Val Met Val Asn Ala Trp Lys Val Gln Arg Asp 385 390 395 400 Pro Ser Val Trp Pro Asn Pro Asp Asp Phe Lys Pro Glu Arg Phe Leu 405 410 415 Lys Lys Asp Ile Asp Phe Arg Asp Gln Asn Phe Glu Phe Ile Pro Phe 420 425 430 Gly Ser Gly Arg Arg Ala Cys Pro Gly Ile Ser Phe Ala Val Glu Leu 435 440 445 Leu Pro Met Ala Leu Ala Arg Leu Leu His Gly Phe Glu Leu Lys Thr 450 455 460 Gln Leu Gly Cys Lys Val Asp Met Thr Glu His Ala Gly Leu Val His 465 470 475 480 Ala Lys Ala Thr Pro Leu Glu Val Leu Val Ser Pro Arg Leu Ser Arg 485 490 495 Glu Leu Tyr Val Ser Ser 500 <210> 271 <211> 529 <212> PRT <213> Menispermum canadense <400> 271 Met Asp Ser Thr Asp His His Leu Ala Leu Val Ile Pro Thr Leu Leu 1 5 10 15 Ala Thr Pro Val Leu Leu Phe Ser Phe Tyr Cys Leu Leu Leu Arg Pro 20 25 30 Arg Ser Leu Lys Lys Pro Arg Thr Asn Lys Pro Pro Glu Pro Ser Gly 35 40 45 Ser Trp Pro Ile Ile Gly His Leu His Leu Leu Ser Gln Gly Leu Pro 50 55 60 His Ile Thr Leu Gly Ala Met Ala Asp Lys Leu Gly Pro Ala Phe Ser 65 70 75 80 Ile Arg Leu Gly Thr Arg Arg Ala Leu Val Val Ser Ser Trp Glu Val 85 90 95 Ala Lys Glu Cys Tyr Thr Thr Asn Asp Arg Ala Phe Ala Ser Arg Pro 100 105 110 Ser Ser Ile Ala Val Lys Leu Met Ser Tyr Asn Asn Ser Ile Leu Gly 115 120 125 Phe Ala Pro Tyr Gly Gln Tyr Trp Arg Glu Leu Arg Lys Ile Val Val 130 135 140 Leu Gln Leu Leu Ser Asn Arg Arg Leu Glu Ser Leu Lys Asn Val Trp 145 150 155 160 Glu Ser Glu Ile Asp Leu Ser Ile Lys Glu Leu Tyr Asp Thr Trp Ala 165 170 175 Ser Asn Arg Gly Gly Glu Ala Leu Met Val Asp Met Lys Gln Trp Phe 180 185 190 Gly Asp Leu Thr Met Asn Ile Val Val Arg Leu Val Val Glu Lys Arg 195 200 205 Cys Phe Gly Arg Ser Val Gly Asp Asp Glu Thr Glu Ala Arg Arg Gly 210 215 220 Gln Arg Ala Leu Lys Glu Phe Leu Lys Leu Val Gly Leu Phe Leu Val 225 230 235 240 Glu Asp Ala Phe Pro Phe Leu Ser Trp Leu Asp Val Gln Gly His Gln 245 250 255 Arg Glu Met Lys Arg Ile Ala Arg Glu Leu Asp Ser Leu Phe Gln Gly 260 265 270 Trp Leu Glu Glu His Lys Arg Lys Arg Ser Ile Lys Gly Glu Ala Lys 275 280 285 Gly Asp Gln Asp Phe Met Asp Val Met Leu Ser Val Leu Glu Asp Ser 290 295 300 Thr Ile Ser Thr Asp Ile Glu Asp Asp Thr Val Ile Lys Ser Thr Cys 305 310 315 320 Leu Thr Val Val Leu Gly Gly Ser Asp Ser Thr Val Gly Thr Leu Thr 325 330 335 Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His Glu Leu Lys Lys Ala 340 345 350 Gln Asp Glu Leu Asp Ala Tyr Val Gly Lys Glu Arg Gln Val Asp Glu 355 360 365 Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Val 370 375 380 Leu Arg Leu Tyr Pro Ala Gly Pro Leu Ser Gly Pro Arg Glu Ser Val 385 390 395 400 Glu Asp Thr Val Val Ala Gly Tyr His Val Pro Lys Gly Thr Gln Leu 405 410 415 Ile Val Asn Leu Trp Lys Ile Gln Arg Glu Pro Ser Ile Trp Ala Asp 420 425 430 Pro Leu Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr His Lys Gly Met 435 440 445 Asp Val Trp Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg 450 455 460 Arg Ser Cys Pro Gly Thr Ala Phe Ala Leu Gln Val Ile His Leu Thr 465 470 475 480 Leu Ala Arg Leu Leu His Gly Phe Glu Leu Thr Thr Pro Cys Gly Ala 485 490 495 Pro Val Asp Met Ser Glu Ser Ala Gly Leu Ile Asn Val Lys Thr Thr 500 505 510 Pro Val Glu Val His Val Ala Pro Arg Leu Pro Leu Lys Leu Tyr Lys 515 520 525 Ser <210> 272 <211> 529 <212> PRT <213> Menispermum canadense <400> 272 Met Ile Leu Ile Ala Met Gly Asn His Ile Glu Leu Gln Asp Ile Ile 1 5 10 15 Leu Tyr Ser Leu Gly Phe Phe Ala Ala Thr Ile Leu Trp Arg Ile Phe 20 25 30 Ser Thr Tyr Val Leu Arg Arg Asn Ser Cys Ser Arg Pro Pro Glu Ala 35 40 45 Ala Gly Lys Leu Pro Leu Ile Gly His Leu His Leu Leu Gly Ala Asn 50 55 60 Lys Ile Leu His His Thr Leu Gly Asp Met Ala Asp Arg His Gly Pro 65 70 75 80 Ile Phe Ser Leu Asn Leu Gly Ile Lys Arg Thr Leu Val Ile Thr Ser 85 90 95 Trp Glu Val Ala Lys Glu Cys Phe Thr Thr Gln Asp Arg Val Phe Ala 100 105 110 Thr Arg Pro Lys Ser Leu Val Gly Lys Val Val Gly Tyr Asn Ser Thr 115 120 125 Val Met Ile Phe Gln Gln Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys 130 135 140 Leu Ala Met Ile Glu Leu Leu Ser Asn Arg Arg Leu Glu Met Leu Lys 145 150 155 160 His Val Arg Glu Ser Glu Val Asn Leu Phe Ile Lys Glu Leu Tyr Glu 165 170 175 Gln Trp Ser Ser Asn Glu Asn Gly Ser Lys Val Val Val Glu Met Lys 180 185 190 Glu Arg Phe Gly Asp Leu Thr Thr Asn Ile Val Val Arg Thr Val Ala 195 200 205 Gly Lys Lys Tyr Ser Gly Thr Gly Val His Gly Asn Glu Glu Ser Arg 210 215 220 Arg Phe Gln Lys Ala Met Thr Asp Phe Met His Leu Ala Gly Leu Phe 225 230 235 240 Met Val Ser Asp Ala Leu Pro Leu Leu Gly Trp Ile Asp Thr Phe Lys 245 250 255 Gly Tyr Arg Gly Lys Met Asn Lys Thr Ala Glu Glu Ile Asp His Val 260 265 270 Leu Gly Ser Trp Leu Lys Glu His Gln Gln Lys Arg Lys Asn Ile Ser 275 280 285 Ile Asn His Leu Asp Glu Asp Phe Ile His Val Met Leu Ser Ala Met 290 295 300 Asp Gly Asn Gln Phe Pro Asp Ile Asp Thr Glu Thr Ala Ile Lys Gly 305 310 315 320 Thr Cys Leu Ser Leu Ile Leu Gly Gly Tyr Asp Thr Thr Ser Ala Thr 325 330 335 Leu Thr Trp Ala Leu Ser Leu Ile Val Asn Asn His His Val Leu Lys 340 345 350 Lys Ala Gln Asp Glu Met Asp Lys Tyr Val Gly Arg Asp Arg Gln Val 355 360 365 Lys Glu Ser Asp Val Lys Asn Leu Thr Tyr Leu Gln Ala Ile Val Lys 370 375 380 Glu Thr Leu Arg Leu Tyr Pro Ala Ala Pro Leu Ser Val Gln His Glu 385 390 395 400 Ala Met Glu Asp Cys Thr Val Ala Gly Cys Asn Ile Pro Ala Gly Thr 405 410 415 Arg Leu Val Val Asn Leu Trp Lys Met His Arg Asp Pro Lys Val Trp 420 425 430 Ser Asp Pro Leu Glu Phe Gln Pro Asp Arg Phe Leu Gln Lys His Val 435 440 445 Asn Val Asp Ile Trp Gly Gln Asn Phe Glu Leu Leu Pro Phe Gly Ser 450 455 460 Gly Arg Arg Ser Cys Pro Gly Ile Thr Phe Ala Ile Gln Val Leu His 465 470 475 480 Leu Thr Leu Ala Gln Leu Leu His Ala Phe Gln Leu Gly Thr Val Phe 485 490 495 Ala Ser Pro Ile Asp Met Thr Glu Ser Ser Gly Val Thr Asn Pro Lys 500 505 510 Ala Thr Pro Leu Gln Val Thr Leu Thr Pro Arg Leu Pro Pro Glu Val 515 520 525 Tyr <210> 273 <211> 513 <212> PRT <213> Menispermum canadense <400> 273 Met Asp Ser Thr Tyr Gln Leu Leu Ser Ala Leu Leu Phe Ile Leu Cys 1 5 10 15 Leu Tyr Tyr Leu Val Cys Lys Pro Ile Thr Lys Thr Lys Asn Asp Glu 20 25 30 Ala Pro Val Pro Ala Gly Ala Trp Pro Ile Met Gly His Leu His Met 35 40 45 Leu Arg Gly Pro Asn Leu Pro His Ile Thr Leu Ser Ser Met Ala Asp 50 55 60 Lys His Gly Pro Ala Phe Thr Ile Arg Leu Gly Thr Arg Arg Ala Leu 65 70 75 80 Val Val Ser Asn Ser Asp Leu Ala Lys Glu Cys Phe Thr Val Asn Asp 85 90 95 Lys Ala Phe Ser Thr Arg Pro Ser Thr Val Ala Thr Lys Leu Met Gly 100 105 110 Tyr Asn Asn Thr Met Phe Gly Phe Ala Pro Tyr Gly Pro Tyr Trp Arg 115 120 125 Glu Leu Arg Lys Ile Val Met Thr Glu Leu Leu Ser Ser Arg Arg Leu 130 135 140 Glu Ser Leu Ala Ser Val Trp Ala Ser Glu Ile Glu Ser Ser Val Lys 145 150 155 160 Glu Leu Phe Ala Glu Trp Ala Glu Asn Arg Ala Lys Gly Pro Val Val 165 170 175 Val Glu Met Gly Glu Trp Phe Gly Asn Leu Thr Leu Asn Ile Ala Leu 180 185 190 Arg Met Val Val Gly Lys Arg Tyr Leu Arg Glu Glu Ser Arg Lys Cys 195 200 205 Leu Lys Ala Met Arg Asp Tyr Pro Glu Leu Phe Gly Arg Phe Leu Val 210 215 220 Glu Asp Ala Val Pro Phe Leu Gly Trp Leu Asp Leu Ile Gln Gly Tyr 225 230 235 240 Gln Arg Glu Met Lys Lys Thr Ala Arg Glu Leu Asp Ser Val Leu Glu 245 250 255 Lys Trp Leu Val Glu His Lys Asp Lys Arg Gly Ser Gly Glu Gly Lys 260 265 270 Gly Glu His Gln Asp Phe Met Asp Val Met Ile Ser Val Leu Glu Asp 275 280 285 Ser Lys Leu Ser Tyr Gly Glu Ala Asp Thr Val Asn Lys Ser Thr Cys 290 295 300 Leu Asn Leu Ile Leu Gly Ala Thr Asp Thr Thr Met Val Ala Leu Thr 305 310 315 320 Trp Ala Leu Ser Leu Leu Leu Asn Asn Lys Gln Val Leu Lys Arg Ala 325 330 335 Gln Gln Glu Leu Lys Ser Gln Ile Gly Asn Asn Lys Gln Val Ser Gln 340 345 350 Ser Asp Ile Lys Asn Leu Leu Tyr Leu Gln Ala Thr Val Lys Glu Ala 355 360 365 Leu Arg Leu Tyr Pro Pro Glu Pro Leu Ser Gly Pro Arg Glu Ala Leu 370 375 380 Glu Asp Cys Thr Val Ala Gly Tyr Arg Val Arg Ala Gly Thr Arg Leu 385 390 395 400 Ile Val Asn Leu Trp Lys Ile His Arg Asp Pro Ser Ile Trp Gln Val 405 410 415 Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Thr Ala His Lys Asp Val 420 425 430 Asp Val Trp Gly Gln His Phe Glu Leu Met Pro Phe Gly Ser Gly Arg 435 440 445 Arg Ser Cys Pro Gly Ile Ser Phe Val Leu Gln Val Val Pro Leu Ile 450 455 460 Leu Ala Arg Leu Leu His Gly Phe Glu Leu Thr Thr Pro Gly Glu Ala 465 470 475 480 Leu Val Asp Met Ser Glu Ser Ala Gly Leu Val Asn Ala Lys Val Thr 485 490 495 Pro Leu Glu Val Val Ile Ser Pro Arg Leu Pro Leu Glu Leu Phe Ala 500 505 510 Gly <210> 274 <211> 534 <212> PRT <213> Menispermum canadense <400> 274 Met Ile Met Met Phe Ile Asp Tyr Tyr Ser Ser Trp Leu Pro Gln Thr 1 5 10 15 Leu Leu Leu Gln Ser Ile Leu Leu Ala Val Ser Leu Val Ile Phe Ile 20 25 30 Asn Leu Phe Leu Thr Arg Arg Arg Ser Tyr Ser Ser Lys Ser His Thr 35 40 45 Asn Ile Ile His Pro Pro Lys Ala Ala Gly Ala Leu Pro Val Ile Gly 50 55 60 His Leu Tyr Thr Leu Phe Arg Gly Leu Ser Ala Gly Val Pro Leu Tyr 65 70 75 80 Arg Gln Leu Asp Ala Met Ala Asp Arg Tyr Gly Pro Ala Phe Ile Ile 85 90 95 His Leu Gly Val Tyr Pro Thr Leu Val Val Thr Cys Arg Glu Leu Ala 100 105 110 Lys Glu Cys Phe Thr Thr Asn Asp Gln Thr Phe Ala Thr Arg Pro Ser 115 120 125 Thr Cys Ala Gly Lys Tyr Ile Gly Tyr Asn Tyr Ala Phe Phe Gly Phe 130 135 140 Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Ala Arg Lys Ile Ala Thr Val 145 150 155 160 Glu Leu Leu Ser Asn Tyr Arg Leu Asp Ser Leu Arg His Val Arg Glu 165 170 175 Ala Glu Val Gly Arg Asn Val Asp Glu Leu Tyr Ala Leu His Ala Ser 180 185 190 Ser Ser Thr Asn Lys Gln Asn Met Met Lys Ile Asp Met Lys Gln Trp 195 200 205 Phe Asp Gln Val Thr Leu Asn Val Ile Leu Met Met Val Val Gly Lys 210 215 220 Arg Cys Val Thr Thr Gly Gly Asn Glu Glu Glu Val Arg Val Val Lys 225 230 235 240 Val Leu His Glu Phe Phe Lys His Leu Gly Thr Leu Ser Val Ser Asp 245 250 255 Val Val Pro Tyr Val Glu Trp Met Asp Leu Asp Gly Asn Ile Gly Arg 260 265 270 Met Lys Ser Thr Ala Lys Glu Leu Asp Cys Ile Leu Gly Arg Trp Leu 275 280 285 Glu Glu His Arg Arg Glu Arg Arg Ser Asp Phe Met Asp Ala Met Leu 290 295 300 Ala Met Val Glu Gly Ile Lys Ile Pro Tyr Tyr Asp Ser Asp Thr Val 305 310 315 320 Ile Lys Ala Ile Cys Leu Asn Leu Leu Asn Ala Gly Ser Asp Thr Leu 325 330 335 Gly Ile Thr Met Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His 340 345 350 Val Leu Lys Lys Val Lys Asp Glu Leu Asp Val His Val Gly Lys Asn 355 360 365 Arg Gln Val Glu Glu Leu Asp Val Lys Asn Leu Val Tyr Leu His Ala 370 375 380 Val Val Lys Glu Thr Leu Arg Leu Phe Pro Pro Ala Pro Leu Gly Val 385 390 395 400 Pro His Glu Ala Met Glu Asp Cys Val Val Gly Gly Phe His Val Ala 405 410 415 Lys Gly Thr Arg Leu Val Val Asn Val Trp Lys Leu His Arg Asp Pro 420 425 430 Ser Val Trp Ser Asp Pro Leu Ala Phe Lys Pro Glu Arg Phe Leu Asp 435 440 445 Asn Asn Thr Val Asp Val Arg Gly Gln His Phe Gln Leu Leu Pro Phe 450 455 460 Gly Ser Gly Arg Arg Gly Cys Pro Gly Ile Thr Phe Ala Leu Gln Val 465 470 475 480 Ala His Leu Thr Leu Ala Arg Leu Leu His Gly Phe Glu Trp Asp Thr 485 490 495 Pro Asp Gly Ala Pro Val Asp Met Ser Glu Val Ser Val Leu Thr Thr 500 505 510 Ala Lys Lys Asn Pro Val Glu Val Leu Phe Thr Pro Arg Leu Pro Ala 515 520 525 Glu Val Tyr Thr Gln Asn 530 <210> 275 <211> 523 <212> PRT <213> Menispermum canadense <400> 275 Tyr Gln Leu Leu Phe Leu Gln Thr Ala Ile Ala Gly Leu Leu Phe Ala 1 5 10 15 Leu Ala Leu Leu Val Arg Leu Arg Phe Ser Arg Arg Asn Asn Gly Lys 20 25 30 Lys Lys Gln Ala Pro Pro Glu Pro Ala Gly Ala Arg Leu Ile Met Gly 35 40 45 His Ile His Met Leu Asn Ser Gly Glu Gln Pro His Lys Thr Leu Ala 50 55 60 Ala Leu Ala Asp Gln Tyr Gly Pro Val Ile Lys Leu Arg Leu Gly Leu 65 70 75 80 Arg Asp Ala Ile Val Val Ser Asn Trp Glu Met Ala Lys Glu Cys Phe 85 90 95 Thr Thr Asn Asp Lys Ile Phe Leu Ser Arg Pro Gln Thr Val Val Ser 100 105 110 Lys Phe Met Ser Tyr Asn Leu Lys Met Leu Gly Phe Ala Pro Tyr Gly 115 120 125 Pro Tyr Trp Arg Glu Ile Arg Lys Ile Val Val Ser Glu Leu Leu Ser 130 135 140 Asn Arg Arg Leu Glu Leu Leu Lys Asn Ala Trp Thr Ser Glu Ile Ser 145 150 155 160 Ser Trp Val Lys Glu Leu Phe Asp Glu Trp Gly Ala Lys Glu Ala Arg 165 170 175 Gly Pro Val Val Val Asp Met Arg His Trp Phe Gly Asn Leu Ala Phe 180 185 190 Asn Ile Gly Met Asn Met Val Ala Gly Lys Arg Phe Phe Gly Pro Arg 195 200 205 Val Val Ser Asp Glu Asp Gly Leu Arg Arg Val Gln Gln Gly Leu Thr 210 215 220 Asp Phe Phe Arg Leu Ile Gly Ala Phe Leu Leu Glu Asp Ala Val Pro 225 230 235 240 Phe Leu Ser Trp Trp Asp Ser Gln Gly Tyr Gln Lys Glu Met Lys Asp 245 250 255 Thr Ala Arg Glu Met Asp Thr Leu Ile Gln Gly Leu Leu Asp Glu His 260 265 270 Lys Arg Arg Arg Ser Ser Ser Ser Gly Glu Ala Lys Gly Glu Lys Asp 275 280 285 Phe Met Asp Val Met Leu Asn Ile Leu Glu Gly Ser Lys Phe Pro Ser 290 295 300 Gly Asp Ala Asp Asn Ile Asn Lys Ser Thr Cys Leu Ser Leu Met Leu 305 310 315 320 Gly Leu Thr Asp Thr Thr Thr Ala Thr Leu Thr Trp Ala Leu Ser Leu 325 330 335 Leu Leu Asn Asp Pro Arg Val Leu Lys Lys Ala Arg Glu Glu Leu Asp 340 345 350 Phe His Val Gly Lys Asn Arg Leu Val Asp Thr Ser Asp Leu Met Asn 355 360 365 Leu Pro Tyr Ile His Ala Ile Val Lys Glu Val Leu Arg Leu His Pro 370 375 380 Pro Ala Pro Leu Ser Gly His Arg Glu Ser Leu Glu Asp Ser Val Val 385 390 395 400 Gly Gly Tyr His Val Pro Lys Gly Thr Arg Phe Met Val Asn Val Trp 405 410 415 Lys Ile His His Asp Pro Asn Lys Trp Pro Asp Pro Ile Glu Phe Arg 420 425 430 Pro Glu Arg Phe Leu Thr Thr His Lys Asp Val Glu Val Trp Gly Gln 435 440 445 Asn Phe Glu Leu Ile Pro Phe Ser Ser Gly Arg Arg Val Cys Pro Gly 450 455 460 Ala Ser Leu Ser Leu Gln Val Leu Pro Phe Thr Leu Ala Arg Val Leu 465 470 475 480 Gln Gly Phe Glu Val Ala Thr Pro Gly Gly Ala Pro Val Asp Val Thr 485 490 495 Glu Ser Lys Gly Leu Ser Thr Ala Lys Glu Thr Pro Leu Asp Val Val 500 505 510 Ile Thr Pro Arg Leu Pro Glu Lys Phe Tyr His 515 520 <210> 276 <211> 528 <212> PRT <213> Nandina domestica <400> 276 Met Asp Ser Leu Ile Ser Phe Gln Ala Ile Val Gly Leu Phe Ile Leu 1 5 10 15 Ile Leu Val Ser Tyr Gln Trp Leu Gly Arg Ser Arg Ser Ile Lys Thr 20 25 30 Asn Lys His Asn Glu Ala Pro Glu Pro Ala Gly Arg Trp Pro Ile Ile 35 40 45 Gly His Leu His Leu Leu Gly Gly Ser Asp Gln Leu Leu Tyr Arg Thr 50 55 60 Leu Gly Ser Met Ala Asp Lys Leu Gly Pro Ala Phe Asn Ile Arg Leu 65 70 75 80 Gly Ser Arg Arg Ala Phe Val Val Asn Ser Trp Glu Val Ala Lys Glu 85 90 95 Cys Phe Thr Ile Asn Asp Lys Ala Leu Ala Ser Arg Pro Ile Thr Val 100 105 110 Ala Ala Lys Leu Met Gly Tyr Asn Tyr Ala Val Phe Gly Phe Ala Pro 115 120 125 Tyr Ser Pro Phe Trp Arg Ala Met Arg Lys Ile Ala Thr Leu Glu Leu 130 135 140 Leu Ser Asn Arg Arg Leu Glu Met Leu Lys His Val Arg Ile Ser Glu 145 150 155 160 Val Asp Met Gly Leu Lys Glu Ile Tyr Gly Leu Trp Ser Lys Asn Lys 165 170 175 Asp Ser Gly Pro Leu Met Ile Glu Leu Asn Arg Trp Phe Glu Asn Leu 180 185 190 Thr Leu Asn Met Val Val Arg Met Val Ala Gly Lys Arg Tyr Phe Gly 195 200 205 Ala Asp Ala Ser Cys Asp Glu Asn Glu Ala Gln Arg Cys Gln Lys Ala 210 215 220 Ile Ser Glu Phe Phe Arg Leu Ile Gly Ile Phe Val Val Ser Asp Ala 225 230 235 240 Ile Pro Ser Leu Trp Trp Leu Asp Leu Gln Gly His Glu Lys Ala Met 245 250 255 Lys Arg Thr Ala Lys Asp Leu Asp Ser Ile Leu Gly Gly Trp Leu Glu 260 265 270 Gln His Arg Ser Arg Arg Val Asn Gly Lys Val Pro Thr Glu Gly Glu 275 280 285 Gln Asp Phe Ile Asp Val Met Leu Ser Leu Gln Glu Gly Asp His Leu 290 295 300 Ser Asp Phe Glu Tyr Asp Ala Asp Ile Ala Ile Lys Ser Thr Cys Leu 305 310 315 320 Ala Leu Ile Leu Gly Gly Ser Asp Thr Thr Ala Gly Thr Leu Thr Trp 325 330 335 Ala Ile Ser Leu Leu Leu Asn His Pro Tyr Ala Leu Lys Lys Ala Gln 340 345 350 Glu Glu Leu Asp Leu His Val Gly Lys Asp Arg Gln Val Tyr Asp Gln 355 360 365 Asp Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile Ile Lys Glu Thr Leu 370 375 380 Arg Leu Tyr Pro Ala Gly Pro Leu Leu Gly Pro Arg Glu Ala Met Glu 385 390 395 400 Asp Cys Thr Ile Ser Gly Phe Asp Val Arg Ala Gly Thr Arg Leu Val 405 410 415 Val Asn Val Trp Lys Ile Gln Arg Asp Pro Asn Val Trp Ser Asn Pro 420 425 430 Ser Glu Phe Ser Pro Glu Arg Phe Leu Thr Ser His Val Asp Val Asp 435 440 445 Val Arg Gly Gln Asn Phe Glu Leu Met Pro Phe Gly Ser Gly Arg Arg 450 455 460 Ser Cys Pro Gly Ala Ser Phe Ala Leu Gln Val Leu His Leu Thr Leu 465 470 475 480 Ala Arg Phe Leu His Gly Phe Gly Leu Ala Asn Pro Leu Gly Lys Pro 485 490 495 Val Asp Met Asn Glu Ser Pro Gly Leu Thr Ile Pro Lys Ala Thr Pro 500 505 510 Leu Asn Val Leu Leu Thr Pro Arg Leu Asp Ser Glu Leu Tyr Gly Cys 515 520 525 <210> 277 <211> 520 <212> PRT <213> Nandina domestica <400> 277 Met Glu Thr Phe Gln Phe Leu Gly Ile Pro Leu Phe Gly Phe Phe Ala 1 5 10 15 Leu Leu Cys Thr Tyr Tyr Leu Val Leu Arg Lys Pro Ser Ser Thr Lys 20 25 30 Ile Arg Glu Pro Pro Arg Pro Ala Gly Ala Trp Pro Ile Ile Gly His 35 40 45 Leu His Leu Leu Ala Arg Gly Asp Leu Pro His Val Thr Leu Gly Lys 50 55 60 Met Ala Asp Lys Tyr Gly Pro Val Phe Lys Leu Lys Leu Gly Val Arg 65 70 75 80 Gln Ala Ile Val Val Ser Asp Trp Glu Val Ala Lys Glu Cys Tyr Thr 85 90 95 Thr Asn Asp Arg Ala Leu Ala Asn Arg Pro Ser Gly Leu Gly Ala Lys 100 105 110 Ile Met Gly Tyr Asn Tyr Ala Leu Ile Gly Ser Ala Pro Tyr Gly Pro 115 120 125 Tyr Trp Arg Asp Leu Arg Lys Ile Ile Thr Leu Glu Val Leu Ser Ser 130 135 140 Arg Arg Leu Glu Ser Leu Lys His Val Trp Asp Ser Glu Ile Ser Thr 145 150 155 160 Ser Val Lys Glu Leu Tyr Gln Ile Trp Ala Asp Gln Asn Lys Ala Gln 165 170 175 Gly Gln Val Ser Val Glu Met Lys Gln Trp Phe Ser Asp Met Thr Leu 180 185 190 Asn Val Ala Val Arg Leu Ala Val Gly Lys Lys Tyr Leu Gly Ala Thr 195 200 205 Ala Asp Cys Glu Lys Asn Lys Ala Val Gln Cys Gln Lys Ala Ile Arg 210 215 220 Asn Phe Phe Arg Leu Ala Gly Leu Phe Val Val Ala Asp Tyr Leu Pro 225 230 235 240 Phe Leu Gly Trp Leu Asp Leu Gly Gly His Glu Lys Glu Met Lys His 245 250 255 Thr Ala Lys Glu Leu Asp Tyr Ile Ala Gln Glu Trp Leu Asp Asp His 260 265 270 Lys Lys Lys Arg Thr Ser Asn Arg Thr Val Asp Glu Pro Gln Asp Phe 275 280 285 Met Asp Val Met Leu Ser Ile Leu Glu Glu Ser Thr Phe Thr Gly Tyr 290 295 300 Asp Val Asp Ile Ile Asn Lys Ser Thr Cys Phe Ala Leu Ile Leu Gly 305 310 315 320 Gly Thr Asp Thr Val Ala Val Thr Leu Thr Trp Ala Leu Cys Leu Leu 325 330 335 Leu Asn Asn Pro His Val Leu Lys Lys Ala Gln Asp Glu Leu Asp Ile 340 345 350 His Val Gly Lys Asp Arg His Val Asn Glu Ser Asp Ile Lys Asn Leu 355 360 365 Val Tyr Leu Gln Ala Met Ile Lys Glu Thr Leu Arg Leu Tyr Pro Ala 370 375 380 Gly Pro Leu Leu Gly Pro Arg Gln Val Ile Glu Asp Cys Thr Ile Ala 385 390 395 400 Gly Tyr His Val Arg Ala Gly Thr Arg Val Ile Val Asn Ala Trp Lys 405 410 415 Phe Gln Arg Asp Pro Ser Ile Trp Ser Asn Pro Cys Glu Phe Gln Pro 420 425 430 Glu Arg Phe Leu Asp Lys Asp Ile Asp Val Lys Gly Gln His Phe Glu 435 440 445 Leu Ile Pro Phe Gly Ala Gly Arg Arg Ala Cys Pro Gly Ile Ser Phe 450 455 460 Ala Leu Gln Val Leu Pro Leu Ala Leu Ala Arg Leu Leu His Gly Phe 465 470 475 480 Glu Leu Lys Asn Pro Ser Glu Ser Gln Val Asp Met Thr Glu Thr Pro 485 490 495 Gly Met Val His Ala Lys Thr Thr Pro Leu Glu Val Leu Ile Thr Pro 500 505 510 Arg Leu Ser Pro Lys Phe Tyr Val 515 520 <210> 278 <211> 495 <212> PRT <213> Nandina domestica <400> 278 Trp Lys Arg Pro Thr Ser Val Lys Tyr Arg Glu Ala Pro Gln Pro Ala 1 5 10 15 Gly Ala Trp Pro Ile Ile Gly His Leu His Leu Leu Ala Arg Gly Asp 20 25 30 Leu His His Ile Thr Leu Gly Ala Met Ala Asp Lys Tyr Gly Pro Ala 35 40 45 Phe Met Met Arg Leu Gly Val His Gln Ala Met Val Val Ser Asp Ser 50 55 60 Glu Ser Ala Lys Glu Cys Ser Thr Thr Asn Asp Arg Val Leu Ala Thr 65 70 75 80 Arg Pro Ser Ser Val Ala Val Lys Leu Met Gly Tyr Asn Tyr Ala Met 85 90 95 Phe Gly Phe Gly Pro Tyr Gly Ser Tyr Trp Arg Glu Ile Arg Lys Ile 100 105 110 Val Ile Leu Glu Val Leu Ser Asn His Arg Leu Glu Ser Leu Lys His 115 120 125 Val Trp Lys Ser Glu Ile Ser Met Ser Thr Lys Glu Leu Tyr Gln Leu 130 135 140 Trp Ile Asn Gln Asn Lys Asp Glu Gly Pro Thr Leu Val Glu Leu Lys 145 150 155 160 Gln Trp Leu Cys Asn Met Thr Leu Asn Ile Gly Val Arg Thr Val Ala 165 170 175 Gly Lys Arg Tyr Phe Gly Ala Ser Ser Cys Asp Ala Asn Glu Ser Asn 180 185 190 Arg Ile Gln Arg Ala Ile Arg Asp Phe Phe Arg Phe Val Gly Leu Phe 195 200 205 Val Val Ser Asp Phe Leu Pro Phe Leu Gly Trp Leu Asp Leu Glu Gly 210 215 220 Tyr Gln Lys Glu Met Lys Ser Ile Ala Arg Glu Leu Asp Ser Ile Leu 225 230 235 240 Gln Gly Trp Leu Asp Glu His Thr Arg Lys Arg Gln Ser Gly Gly Thr 245 250 255 Asn Glu Asn Gln Asp Phe Met Asp Ile Met Leu Ser Val Leu Glu His 260 265 270 Ser Asn Ala Leu Thr His Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr 275 280 285 Cys Phe Thr Met Ile Leu Gly Gly Ser Asp Thr Thr Met Val Thr Leu 290 295 300 Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Pro His Val Leu Lys Lys 305 310 315 320 Ala Gln Asp Glu Ile Asp Ile Gln Val Gly Lys Asp Arg Leu Val Asp 325 330 335 Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile Val Lys Glu 340 345 350 Thr Leu Arg Leu Tyr Pro Ala Val Pro Ile Ile Thr Pro His Glu Ala 355 360 365 Ile Glu Asp Cys Thr Ile Ala Gly Tyr His Val Pro Ala Gly Thr Arg 370 375 380 Leu Ile Val Asn Ala Trp Lys Ile Gln Arg Asp Pro Leu Ile Trp Asp 385 390 395 400 Asn Pro Gly Glu Phe Gln Pro Glu Arg Phe Leu Tyr Lys Asp Val Asp 405 410 415 Val Lys Gly Lys His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg Arg 420 425 430 Val Cys Pro Gly Ile Ser Leu Ala Leu Gln Val Val Ser Leu Ala Met 435 440 445 Ala His Leu Leu His Glu Phe Asp Leu Ala Lys Pro Ser Glu Gly Asn 450 455 460 Val Asp Met Thr Glu Ser Val Gly Leu Val Asn Ala Lys Ala Thr Pro 465 470 475 480 Leu Glu Val Leu Ile Thr Pro Arg Leu Ser Pro Lys Phe Tyr Glu 485 490 495 <210> 279 <211> 525 <212> PRT <213> Nigella sativa <400> 279 Met Gly Ser Phe Asn Gln Ile Asn Pro Ser Val Val Tyr Phe Ala Ala 1 5 10 15 Phe Thr Ile Phe Phe Phe Phe Phe Leu Tyr Lys Ile Leu Tyr Lys Arg 20 25 30 Arg Thr Ser Ala Lys Thr Arg Val Ala Pro Glu Pro Ala Gly Ala Trp 35 40 45 Pro Phe Ile Gly His Leu Pro Leu Leu Ser Gln Gln Asn Leu Pro His 50 55 60 Val Thr Leu Gly Val Leu Ala Asp Lys Tyr Gly Pro Ala Phe Thr Val 65 70 75 80 Gln Leu Gly Leu His Lys Ala Val Val Val Ser Ser Trp Glu Val Ala 85 90 95 Lys Glu Cys Phe Thr Val Asn Asp Lys Val Leu Ala Thr Arg Pro Ser 100 105 110 Ser Val Ala Val Lys Ile Met Cys Tyr Asn Tyr Ala Val Phe Gly Phe 115 120 125 Gly Pro Tyr Gly Ser Tyr Trp Arg Glu Ala Arg Lys Ile Val Val Arg 130 135 140 Glu Leu Leu Ser Asn His Arg Leu Asn Leu Leu Lys His Val Leu Val 145 150 155 160 Thr Glu Ile Ser Met Ser Met Asn Glu Leu Tyr Thr Val Trp Gln Lys 165 170 175 Asn Ala Asn Asn Val Ser Gly Lys Ala Leu Val Glu Met Lys Gln Trp 180 185 190 Phe Gly Asp Leu Ser Leu Asn Met Ile Val Lys Ile Val Ala Gly Lys 195 200 205 Arg Tyr Phe Gln Ala Ser Ala Asn Ser Asp Glu Arg Asp Gln Leu Lys 210 215 220 Arg Gly Val Gln Asp Leu Phe His Leu Val Gly Leu Phe Leu Val Ser 225 230 235 240 Asp Ala Leu Pro Phe Leu Ser Trp Leu Asp Ile Gly Gly His Glu Lys 245 250 255 Ala Met Arg Arg Thr Ala Lys Glu Leu Asp Asn Ile Leu Gly Ser Trp 260 265 270 Leu Asp Glu His Lys Gln Ala Lys Leu Ala Gly Ala Thr Lys Gly Glu 275 280 285 Gln Asp Phe Met Asp Val Leu Met Ser Ile Leu Glu Asp Asn Asn Asn 290 295 300 Leu Pro Glu Tyr Glu Pro Asp Val Val Ser Lys Ala Ile Cys Leu Gly 305 310 315 320 Met Ile Leu Gly Gly Ala Asp Thr Thr Thr Ile Thr Leu Thr Trp Ile 325 330 335 Val Ser Leu Leu Leu Asn Asn Gln His Ile Leu Lys Lys Ala Gln Asp 340 345 350 Glu Ile Asp Ser Lys Val Gly Lys Asp Arg Gln Val Asn Glu Ser Asp 355 360 365 Ile Glu Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 370 375 380 Leu Tyr Pro Pro Ala Pro Leu Ser Thr Gln His Glu Ala Met Glu Asp 385 390 395 400 Cys Thr Ile Ala Gly Tyr His Val Gln Ala Gly Thr Arg Leu Ile Thr 405 410 415 Asn Ile Trp Lys Ile Gln Arg Asp Pro Gly Val Trp Pro Asn Pro Cys 420 425 430 Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr His Ala Asn Val Glu Ser 435 440 445 Asn Gly Lys His Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Ala 450 455 460 Cys Pro Gly Met Ser Leu Gly Leu Gln Val Val His Leu Thr Leu Ala 465 470 475 480 Arg Leu Leu Gln Gly Phe Glu Leu Glu Thr Pro Leu Ser Val Glu Val 485 490 495 Asp Met Thr Glu Ser Ala Gly Leu Thr Asn Leu Lys Val Thr Pro Leu 500 505 510 Glu Val Leu Ile Asn Pro Arg Leu Pro Ser Asn Leu Tyr 515 520 525 <210> 280 <211> 517 <212> PRT <213> Nigella sativa <400> 280 Met Asp Leu Thr Ser Ile Leu Ile Cys Ile Phe Leu Phe Ile Ile Ser 1 5 10 15 Ile Cys Phe Leu Ser Trp Lys Arg Ser Arg Ser Leu Ser Gly Thr Arg 20 25 30 Ala Ala Pro Glu Pro Val Gly Ala Trp Pro Val Ile Gly His Leu Arg 35 40 45 Leu Leu Met Gly Ser Lys Thr Pro His Met Thr Leu Gly Asn Leu Ala 50 55 60 Asp Asn Tyr Gly Pro Ala Phe Thr Ile Arg Leu Gly Thr Lys Lys Thr 65 70 75 80 Leu Val Val Ser Ser Trp Glu Val Ala Lys Glu Cys Leu Thr Thr Asn 85 90 95 Asp Lys Ala Phe Ala Gly Arg His Ser Thr Met Ala Val Glu Ile Met 100 105 110 Gly Tyr Asn Tyr Ala Phe Phe Ser Leu Gly Pro Ser Gly Gln Tyr Trp 115 120 125 Arg Glu Ile Arg Lys Ile Thr Val Ser Glu Leu Leu Ser Asn Arg Arg 130 135 140 Leu Glu Leu Leu Lys His Ile Trp Ser Ser Glu Ile Lys Thr Ser Ile 145 150 155 160 Lys Glu Leu Tyr Glu Ile Cys Ala Ala Gln Ser Gln Tyr Gly Lys Lys 165 170 175 Gln Ser Pro Met Val Glu Met Gln Gln Trp Phe Ser His Leu Asn Leu 180 185 190 Asn Leu Ser Ala Arg Met Val Val Gly Lys Arg Tyr Phe Ala Asp Gly 195 200 205 Val Glu Glu Asn Glu Gly Asp Ile Arg Arg Phe Gln Asn Ala Ile Lys 210 215 220 Glu Phe Phe Tyr Trp Ala Gly Ala Phe Ile Ile Ala Asp Thr Ile Pro 225 230 235 240 Tyr Leu Arg Trp Met Asp Phe Leu Asn Glu Arg Ser Met Lys Arg Thr 245 250 255 Ala Lys Glu Thr Asp His Val Leu Glu Gly Trp Leu Gln Glu His Lys 260 265 270 Arg Asn Arg Leu Asn Gly Val Thr Lys Glu Gln Asp Phe Met Asp Val 275 280 285 Leu Leu Thr Glu Leu Gly Asp Lys Asp Leu Cys Gly Tyr Thr Ala Asp 290 295 300 Val Val Asn Lys Ala Thr Cys Leu Asn Met Ile Phe Gly Gly Thr Asp 305 310 315 320 Thr Thr Lys Thr Gly Met Ile Trp Thr Leu Ser Leu Ile Leu Asn Asn 325 330 335 Pro Gln Val Leu Lys Lys Leu Gln Asn Glu Ile Asp Ile Gln Val Gly 340 345 350 Lys Asp Arg Gln Val Asp Asp Ser Asp Ile Gly Asn Leu Val Tyr Leu 355 360 365 Gln Ala Thr Val Lys Glu Ala Met Arg Leu Tyr Pro Pro Ala Thr Ile 370 375 380 Phe Gly Arg Glu Ser Ile Glu Asp Cys Thr Val Ala Gly Tyr His Ile 385 390 395 400 Gln Ala Gly Thr Arg Leu Ile Val Asn Ala Trp Lys Ile Gln Arg Asp 405 410 415 Pro Asn Val Trp Pro Asn Pro Asp Glu Phe Gln Pro Glu Arg Phe Leu 420 425 430 Thr Asn Gln Ala Asp Val Asp Phe Arg Gly Gln Asp Phe Glu Leu Ile 435 440 445 Pro Phe Gly Ala Gly Arg Arg Ala Cys Pro Gly Ile Ser Leu Thr Val 450 455 460 Arg Val Thr His Leu Thr Leu Ala Cys Leu Leu Gln Gly Phe Asn Phe 465 470 475 480 Glu Thr Pro Leu Asn Glu Pro Val Asp Met Ser Glu Lys Tyr Gly Leu 485 490 495 Thr Asn Ser Lys Ala Thr Pro Leu Asn Val Ile Leu Thr Pro Arg Leu 500 505 510 Pro Ser Asn Leu Tyr 515 <210> 281 <211> 530 <212> PRT <213> Nigella sativa <400> 281 Met Asp Ser Ile Phe His Met Tyr Ala Pro Ile Asn Ile Leu Ala Ala 1 5 10 15 Thr Phe Val Phe Ile Val Ile Ser Val Trp Phe Leu Leu Trp Met Lys 20 25 30 Arg Ser Arg Lys Asn Ser Lys Ser Arg Arg Ala Pro Glu Pro Val Gly 35 40 45 Gly Trp Pro Leu Ile Gly His Leu Arg Leu Leu Val Gly Pro Glu Leu 50 55 60 Pro His Val Ile Leu Gly Ser Leu Ala Asp Lys Tyr Gly Pro Ala Leu 65 70 75 80 Ser Ile Arg Leu Gly Met Arg Gln Ala Leu Val Val Ser Asn Cys Glu 85 90 95 Val Val Lys Glu Cys Leu Thr Thr Asn Asp Arg Cys Phe Ser Thr Arg 100 105 110 Pro Ser Ser Val Ala Val Glu Leu Met Gly Tyr Asn Tyr Ala Ser Phe 115 120 125 Gly Leu Gly Pro Tyr Gly Gly Phe Trp Arg Glu Val Arg Lys Ile Ala 130 135 140 Ile Leu Glu Leu Leu Ser Asn His Arg Leu Lys Ser Leu Lys His Val 145 150 155 160 Trp Ile Ser Glu Ile Ser Gly Phe Val Lys Glu Leu Tyr Gln Leu Cys 165 170 175 Val Ala Asn Gly Asn Thr Gly Arg Gln Ser Ala Leu Val Glu Met Arg 180 185 190 Gln Trp Leu Asn Asp Leu Thr Leu Asn Val Ser Val Arg Met Val Val 195 200 205 Gly Lys Arg Tyr Phe Gly Ser Gly Ser Glu His Gly Gly Glu Asp Val 210 215 220 Lys Arg Phe Gln Lys Ala Ile Lys Asp Phe Phe Arg Leu Ala Gly Lys 225 230 235 240 Phe Thr Leu Ala Asp Ala Ile Pro Phe Leu Arg Trp Leu Asp Phe Gly 245 250 255 His Glu Arg Ala Met Lys Lys Thr Ala Lys Glu Leu Asp Tyr Val Leu 260 265 270 Ala Arg Trp Leu Asp Gln His Gln Lys Asn Lys Leu Asn Cys Glu Thr 275 280 285 Lys Gln Val Asp Gln Asp Phe Met Asp Val Met Leu Thr Val Leu Gly 290 295 300 Asp Lys Asp Leu Tyr Gly Phe Lys Ala Asp Val Val Asn Lys Ala Thr 305 310 315 320 Cys Leu Asn Leu Ile Leu Gly Gly Val Asp Thr Thr Ser Val Thr Leu 325 330 335 Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Pro Asn Ile Leu Asn Lys 340 345 350 Ala Gln Glu Glu Ile Asp Val Gln Ile Gly Lys Asp Arg Gln Val Asp 355 360 365 Asp Asn Asp Leu Gly Lys Leu Val Tyr Leu Glu Ala Ile Val Lys Glu 370 375 380 Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ser Gly Ala Arg Ala Ala 385 390 395 400 Ile Glu Asp Cys Met Val Ala Gly Tyr His Val Pro Lys Gly Thr Arg 405 410 415 Leu Ile Val Asn Thr Trp Lys Ile Gln Arg Asp Pro Glu Lys Trp Asp 420 425 430 Asn Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Ile Thr Thr Thr Asn 435 440 445 Gly His Val Glu Val Trp Gly Gln His Phe Glu Tyr Ile Pro Phe Gly 450 455 460 Ser Gly Arg Arg Ile Cys Pro Ala Ile Ser Phe Ser Leu Gln Leu Val 465 470 475 480 His Leu Thr Leu Ala Arg Leu Leu Gln Ala Phe Ser Phe Gln Thr Pro 485 490 495 Ser Asn Ala Ala Val Asp Met Thr Glu Thr Pro Gly Leu Val Asn Phe 500 505 510 Lys Ala Thr Pro Leu Gln Ile Leu Leu Thr Pro Arg Leu Pro Ser Ser 515 520 525 Leu Tyr 530 <210> 282 <211> 527 <212> PRT <213> Nigella sativa <400> 282 Met Leu Ser Ile His Asp Ser Thr Met Val Phe Leu Gln Leu Gln Ala 1 5 10 15 Ile Cys Gly Ile Phe Gly Phe Ile Phe Ile Ile Thr Trp Trp Thr Arg 20 25 30 Trp Lys Ser Ser Asn Lys Met Lys Ala Pro Glu Val Ala Gly Ala Trp 35 40 45 Pro Val Ile Gly His Leu His Leu Leu Gly Gly Gly Arg Pro Leu Tyr 50 55 60 Gln Leu Leu Gly Asp Met Ser Asp Lys Tyr Gly Pro Ala Phe Thr Leu 65 70 75 80 Arg Met Gly Ile Gln Lys Ala Leu Val Val Ser Ser Trp Glu Val Ala 85 90 95 Lys Glu Cys Leu Thr Thr Asn Asp Arg Ala Leu Ala Thr Arg Pro Ser 100 105 110 Ser Ala Gly Gly Lys Tyr Met Gly Tyr Asn Asn Ala Leu Ile Pro Phe 115 120 125 Ser Pro Tyr Gly Pro Tyr Trp Arg Asp Met Arg Lys Ile Ala Thr Leu 130 135 140 Glu Leu Leu Ser Asn His Arg Leu Glu Glu Leu Lys His Val Arg Glu 145 150 155 160 Met Glu Ile Asn Thr Cys Ile Ser Asp Met Tyr Lys Leu Cys Gln Val 165 170 175 Glu Asp Gly Val Glu Ile Lys Pro Ile Ser Val Asp Leu Ser Gln Trp 180 185 190 Phe Ala Asp Leu Thr Phe Asn Val Val Val Met Met Ile Thr Gly Lys 195 200 205 Arg Tyr Ile Gly Ser Thr Asp Ala Gly Asp Met Asn Glu Ile Arg His 210 215 220 Phe Gln Ala Ala Leu Val Lys Phe Met Arg Leu Leu Arg Ile Ser Leu 225 230 235 240 Leu Val Asp Val Phe Pro Val Leu Gln Trp Ile Asn Tyr Gly Gly Phe 245 250 255 Lys Gly Val Met Lys Ser Thr Ala Arg Asp Ile Asp Ser Val Leu Glu 260 265 270 Asn Trp Leu Gln Glu His Gln Arg Lys Arg Leu Ser Pro Asp Phe Asn 275 280 285 Gly Asn His Asp Phe Ile Asp Val Met Ile Ser Thr Leu Glu Gly Thr 290 295 300 Glu Phe Ser Asp Tyr Asp His Asn Thr Ile Ile Lys Ala Ile Ser Met 305 310 315 320 Ala Met Val Val Gly Gly Thr Asp Thr Thr Thr Thr Thr Leu Ile Trp 325 330 335 Ala Ile Ser Leu Leu Leu Asn Asn Pro Asn Ala Met Lys Lys Val Gln 340 345 350 Glu Glu Leu Glu Ile His Val Gly Lys Glu Arg Asn Val Asp Gly Ser 355 360 365 Asp Ile Gln His Leu Val Tyr Leu Gln Ala Val Val Lys Glu Thr Leu 370 375 380 Arg Leu Tyr Pro Pro Val Pro Leu Ser Val Met His Gln Ala Met Glu 385 390 395 400 Asp Cys Val Ile Gly Ser Tyr Asn Ile Gln Ala Gly Thr Arg Val Leu 405 410 415 Phe Asn Leu Trp Lys Leu His Arg Asp Ser Ser Val Trp Ser Asp Pro 420 425 430 Leu Glu Phe Arg Pro Glu Arg Phe Leu Thr Ser His Val Asp Val Asp 435 440 445 Val Arg Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg Arg 450 455 460 Ser Cys Pro Gly Ile Ser Phe Ala Leu Gln Val Ile His Leu Thr Ile 465 470 475 480 Ala Arg Leu Phe His Gly Phe Asn Leu Thr Thr Pro Gly Asn Ser Ser 485 490 495 Val Asp Met Ser Glu Ile Ser Gly Ala Thr Leu Ser Lys Val Thr Pro 500 505 510 Leu Glu Val Leu Val Thr Pro Arg Leu Ser Ser Lys Leu Tyr Asn 515 520 525 <210> 283 <211> 520 <212> PRT <213> Nigella sativa <400> 283 Met Asp Phe Leu Ile Tyr Val Ala Ala Phe Ala Ala Ala Phe Phe Phe 1 5 10 15 Leu Leu Phe Tyr Asn Ile Leu Ser Lys Arg Ile Thr Gly Thr Lys Arg 20 25 30 Arg Ile Ala Pro Glu Pro Ala Gly Ala Trp Pro Phe Ile Gly His Leu 35 40 45 Pro Met Leu Phe Gly Pro Asn Leu Pro His Val Thr Leu Gly Ala Leu 50 55 60 Ala Asp Lys Tyr Gly Pro Ala Phe Thr Ile Gln Leu Gly Leu His Lys 65 70 75 80 Ala Leu Val Val Ser Ser Trp Glu Val Ala Lys Asp Cys Phe Thr Val 85 90 95 Asn Asp Lys Ala Leu Ala Thr Arg Pro Thr Ser Ala Ala Val Lys Ile 100 105 110 Met Ala Tyr Asn Cys Ala Val Phe Ser Phe Ser Pro Tyr Gly Ser Tyr 115 120 125 Trp Arg Glu Ala Arg Lys Ile Ala Ile Leu Glu Leu Leu Ser His His 130 135 140 Arg Leu Asp Leu Leu Lys His Val Arg Lys Ser Glu Val Ser Thr Ser 145 150 155 160 Ile Arg Glu Leu Tyr Gln Phe Trp Gln Lys Asn Ala Thr Glu Val Thr 165 170 175 Lys Phe Ala Gln Leu Glu Met Lys Gln Trp Phe Gly Asp Leu Thr Met 180 185 190 Asn Leu Ile Val Arg Met Val Ala Gly Lys Arg Tyr Ala Ser Val Gln 195 200 205 Ser Asp Asp Asp Gly Glu Gly Lys Arg Leu Gln Lys Ala Val Asn Asp 210 215 220 Phe Phe His Leu Val Gly Leu Phe Val Val Ser Asp Ala Leu Pro Phe 225 230 235 240 Leu Gly Trp Leu Asp Ile Gly Gly Asn Leu Lys Thr Met Lys Lys Thr 245 250 255 Ala Arg Glu Leu Asp Ser Ile Met Glu Cys Trp Leu Lys His Gln Ala 260 265 270 Arg Arg Ser Asn Gly Glu Gln Asp Phe Leu Asp Val Met Leu Ser Ile 275 280 285 Leu Arg Glu Asp Asn Lys Leu Gln Glu Tyr Asp Gly Asp Val Val Val 290 295 300 Lys Ala Met Cys Leu Asn Met Ile Leu Gly Gly Ala Asp Ser Thr Thr 305 310 315 320 Ile Thr Leu Thr Trp Thr Ile Ser Leu Leu Leu Asn Asn Arg His Ile 325 330 335 Leu Gln Lys Ala Gln Asp Glu Ile Asp Ser Lys Val Gly Lys Asp Arg 340 345 350 Gln Val Asn Glu Ser Asp Ile Glu Lys Leu Val Tyr Leu Gln Ala Ile 355 360 365 Val Lys Glu Thr Leu Arg Leu Tyr Pro Pro Ala Pro Leu Ser Ser Gln 370 375 380 His Glu Ala Ile Glu Asp Cys Thr Ile Ala Gly Tyr His Ile Pro Ala 385 390 395 400 Gly Thr Arg Leu Ile Thr Asn Leu Trp Lys Ile Gln His Asp Pro Ser 405 410 415 Val Trp His Asn Pro Ser Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr 420 425 430 Gln Ala Asn Val Asp Phe Arg Gly Gln Asn Phe Glu Phe Ile Pro Phe 435 440 445 Gly Phe Gly Arg Arg Ser Cys Pro Gly Thr Ser Leu Gly Leu Gln Met 450 455 460 Val His Leu Pro Leu Ala Arg Leu Leu Gln Gly Phe Thr Phe Glu Thr 465 470 475 480 Pro Ser Asn Ala Ala Val Asp Met Thr Glu Ser Ala Gly Leu Thr Asn 485 490 495 His Lys Val Thr Pro Leu Glu Val Leu Ile Ser Pro Arg Leu Arg Phe 500 505 510 Asp Leu Tyr Gln Glu Ser Pro Pro 515 520 <210> 284 <211> 525 <212> PRT <213> Nigella sativa <400> 284 Ser Phe Asn Gln Thr Tyr Thr Gly Phe Ile Cys Leu Ser Val Phe Ala 1 5 10 15 Ser Phe Phe Phe Phe Phe Leu Tyr Ser Val Gln Gln Lys Arg Arg Thr 20 25 30 Gly Arg Lys Ala Arg Tyr Ala Pro Glu Pro Thr Gly Ala Trp Pro Leu 35 40 45 Ile Gly His Leu Pro Ile Leu Phe Glu Pro Asn Leu Pro His Ile Gln 50 55 60 Leu Gly Ala Leu Ala Asp Lys Tyr Gly Pro Val Phe Ser Ile Lys Leu 65 70 75 80 Gly Leu Arg Lys Ala Leu Val Val Ser Ser Trp Glu Val Val Lys Glu 85 90 95 Cys Phe Thr Val Lys Asp Lys Val Leu Ala Ser Arg Pro Ser Ser Val 100 105 110 Ala Val Lys Ile Met Gly Tyr Asp Tyr Ala Val Tyr Ala Phe Gly Pro 115 120 125 Tyr Gly Ser Tyr Trp Arg Glu Ala Arg Lys Leu Thr Ile Leu Glu Leu 130 135 140 Leu Ser Asp His Arg Leu Asp Leu Leu Lys His Val Arg Val Ser Glu 145 150 155 160 Val Ser Met Ser Met Lys Glu Leu Leu Lys Phe Cys Gln Lys Asn Ala 165 170 175 Asn Asn Val Ser Gly Lys Ala Leu Leu Val Asp Met Lys Gln Trp Phe 180 185 190 Gly Asp Leu Thr Leu Asn Val Ile Val Arg Met Val Ala Gly Lys Ser 195 200 205 Tyr Leu Gly Ala Ser Ala Lys Leu Glu Asp Gly Glu Gln Lys Arg Leu 210 215 220 Gln Asn Ala Ile His Glu Phe Phe Arg Leu Ala Gly Leu Phe Val Val 225 230 235 240 Ser Asp Ala Leu Pro Ile Leu Ser Trp Leu Asp Ile Gly Gly His Glu 245 250 255 Lys Ala Met Arg Arg Asn Ala Arg Glu Ile Asp Lys Ile Met Ser Ser 260 265 270 Trp Leu Asp Glu His Arg Arg Asn Lys Leu Ala Gly Val Thr Asn Gly 275 280 285 Glu Gln Asp Phe Met Asp Val Leu Leu Ser Lys Phe Glu Asn Ser Asn 290 295 300 Leu Pro Gly Tyr Glu Pro Asp Val Ala Ile Lys Ala Ile Cys Leu Asn 305 310 315 320 Met Ile Leu Gly Ala Ala Asp Thr Thr Ile Val Thr Leu Thr Trp Val 325 330 335 Val Ser Leu Ile Leu Asn Asn Arg Gln Ile Leu Lys Lys Ala Arg Glu 340 345 350 Glu Ile Glu Asn Lys Val Gly Lys Asp Arg Gln Val Asn Glu Ser Asp 355 360 365 Ile Glu Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 370 375 380 Leu Tyr Pro Ala Ala Pro Leu Ser Ala His His Glu Ser Met Glu Asp 385 390 395 400 Cys Thr Ile Ala Gly Tyr His Val Pro Ala Gly Thr Gln Leu Ile Thr 405 410 415 Asn Leu Trp Lys Ile Gln Arg Asp Pro Arg Val Trp Pro Asn Pro Cys 420 425 430 Glu Phe Leu Pro Glu Arg Phe Leu Thr Thr His Ala Asn Val Asn Tyr 435 440 445 Lys Gly Gln Asn Phe Glu Phe Ile Pro Phe Gly Tyr Gly Arg Arg Ser 450 455 460 Cys Pro Gly Met Ser Phe Gly Leu Gln Met Val His Leu Val Leu Ala 465 470 475 480 Ser Leu Leu Gln Gly Phe Glu Leu Glu Thr Pro Leu Asn Glu Ala Val 485 490 495 Asp Met Thr Glu Ser Ala Gly Leu Thr Asn Leu Lys Ala Thr Pro Leu 500 505 510 Glu Val Leu Ile Thr Pro Arg Met Pro Leu Asn Leu Tyr 515 520 525 <210> 285 <211> 509 <212> PRT <213> Nigella sativa <400> 285 Val Thr Val Ile Tyr Leu Phe Thr Phe Ala Ile Phe Phe Tyr Leu Tyr 1 5 10 15 Ser Ile Leu Trp Lys Asn Pro Arg Lys Ser Ala Lys Thr Arg Val Ala 20 25 30 Pro Glu Pro Ala Gly Ala Trp Pro Phe Met Gly His Leu Pro Met Leu 35 40 45 Leu Glu Pro Asn Leu Pro His Ile Lys Leu Gly Ala Leu Ala Asp Lys 50 55 60 Tyr Gly Pro Ala Phe Thr Val Gln Leu Gly Leu His Lys Ala Leu Val 65 70 75 80 Val Ser Ser Trp Glu Val Ala Lys Glu Cys Phe Thr Val Asn Asp Lys 85 90 95 Val Leu Ala Asp Arg Pro Ser Ser Val Ala Val Lys Ile Met Gly Tyr 100 105 110 Asp Asn Ala Val Phe Ala Phe Gly Pro Tyr Gly Ser Tyr Trp Arg Glu 115 120 125 Ala Arg Lys Ile Ser Thr Val Glu Leu Leu Ser Asn His Arg Leu Ser 130 135 140 Leu Leu Gln His Phe Arg Ile Ser Glu Ala Ser Met Ser Met Lys Glu 145 150 155 160 Leu Tyr Gln Leu Cys Ala Asn Asn Gly Lys Ala Leu Val Glu Met Lys 165 170 175 Lys Trp Phe Gly Asp Leu Ser Leu Asn Val Ile Val Arg Met Val Ala 180 185 190 Gly Lys Arg Tyr Ala Lys Ala Ser Glu Asp Asp Glu Arg Arg Gln Leu 195 200 205 Gln Lys Gly Leu Lys Asp Phe Phe Tyr Leu Val Gly Leu Phe Val Val 210 215 220 Ser Asp Ala Leu Pro Ile Leu Ser Trp Leu Asp Ile Gly Gly His Glu 225 230 235 240 Lys Ala Met Arg Arg Thr Ala Lys Glu Leu Asp Thr Ile Met Glu Arg 245 250 255 Trp Leu Asp Glu His Arg Arg Ala Lys Leu Ala Gly Val Thr Lys Gly 260 265 270 Glu Gln Asp Phe Met Asp Val Leu Leu Ser Ile Leu Glu Asp Gly Lys 275 280 285 Leu Pro His Tyr Glu Ser Asn Thr Val Asn Lys Ala Ile Cys Leu Thr 290 295 300 Met Ile Leu Gly Ala Ala His Thr Thr Thr Val Thr Leu Ile Trp Thr 305 310 315 320 Leu Ser Leu Leu Leu Asn Asn Arg His Ile Leu Asn Lys Ala Gln Asp 325 330 335 Glu Ile Asp Ser Glu Val Gly Lys Asp Arg Ala Val Thr Glu Ser Asp 340 345 350 Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 355 360 365 Leu Tyr Pro Ala Ala Pro Leu Pro Ala His Arg Glu Val Met Glu Asp 370 375 380 Cys Ile Ile Ala Gly Tyr His Val Pro Val Gly Thr Arg Ile Ile Thr 385 390 395 400 Asn Leu Trp Lys Ile Gln Arg Asp Pro Arg Val Trp Ser Asn Pro Cys 405 410 415 Ala Phe Gln Pro Glu Arg Phe Leu Thr Thr His Ser Asn Val Asp Phe 420 425 430 Arg Gly Gln Asn Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser 435 440 445 Cys Pro Gly Ile Ser Leu Gly Leu Gln Met Val His Leu Ala Leu Ala 450 455 460 Arg Leu Leu Gln Gly Phe Glu Leu Glu Thr Pro Leu Asn Leu Gly Ile 465 470 475 480 Asp Met Thr Glu Ser Ser Gly Leu Thr Asn Leu Lys Val Thr Pro Leu 485 490 495 Glu Val Val Ile Thr Pro Arg Leu Pro Ser Asn Leu Tyr 500 505 <210> 286 <211> 562 <212> PRT <213> Papaver bracteatum <400> 286 Met Ala Tyr Leu Met Ile Lys Lys Ser Phe His Leu Phe Ser Asp Gln 1 5 10 15 Pro Thr Ser Val Ser Thr Leu Ile Val Leu Ala Phe Leu Leu Thr Leu 20 25 30 Ser Pro Val Ile Ile Tyr Tyr Glu Gln Lys Lys Arg Gly Leu Arg Arg 35 40 45 Asn Arg Thr Ser Ser Ser Cys Thr Ala Ile Thr Thr Thr Pro Leu Pro 50 55 60 Glu Ala Ser Gly Ala Trp Pro Val Ile Gly His Leu Leu Leu Phe Met 65 70 75 80 Asn Glu Asn Asp Leu Asn His Ala Thr Leu Gly Asn Met Ala Asp Lys 85 90 95 Tyr Gly Pro Ile Phe Ser Leu Arg Phe Gly Ser His Arg Thr Leu Val 100 105 110 Val Ser Ser Trp Glu Met Val Lys Glu Cys Phe Thr Gly Ala Asn Asp 115 120 125 Lys Phe Phe Ser Asn Arg Pro Ser Ser Leu Ala Val Lys Leu Met Phe 130 135 140 Tyr Asp Thr Glu Ser Tyr Gly Phe Ala Pro Tyr Gly Lys Tyr Trp Arg 145 150 155 160 Glu Leu Arg Lys Ile Ser Thr His Lys Leu Leu Ser Asn Gln Gln Leu 165 170 175 Asp Lys Phe Lys His Leu Arg Ile Ser Glu Val Asp Asn Ser Phe Lys 180 185 190 Lys Leu His Asp Leu Cys Ser Asn Asn Lys Leu Gly Gly Glu Thr Thr 195 200 205 Ser Val Ala Asn Leu Val Arg Met Asp Asp Trp Phe Ala Tyr Leu Thr 210 215 220 Phe Asn Val Ile Gly Arg Ile Val Ser Gly Phe Gln Ser Asn Ala Val 225 230 235 240 Ser Gly Ala Thr Ser Ser Gln Glu Lys Tyr Lys Leu Ala Ile Asp Glu 245 250 255 Val Ser Asn Leu Met Ala Thr Phe Ala Val Ser Asp Val Val Pro Cys 260 265 270 Leu Gly Trp Ile Asp Arg Leu Thr Gly Leu Thr Gly Lys Met Lys Lys 275 280 285 Cys Gly Lys Lys Leu Asp Ala Val Val Gly Asp Ala Val Glu Val His 290 295 300 Arg Gln Lys Lys Leu Lys Ile Ser Arg Asn Thr Ala Gly Glu Leu Thr 305 310 315 320 Glu His Glu Glu Glu Asp Phe Ile Asp Val Cys Leu Ser Ile Met Glu 325 330 335 Gln Ser Gln Ile Pro Gly Asn Asn Pro Glu Ile Ser Val Lys Ser Ile 340 345 350 Ala Leu Asp Met Leu Ser Gly Gly Ser Asp Thr Thr Lys Leu Ile Met 355 360 365 Thr Trp Thr Leu Ser Leu Leu Leu Asn His Pro Asp Ile Leu Asp Lys 370 375 380 Ala Lys Glu Glu Val Asp Thr Tyr Phe Arg Lys Lys Lys Ile Ser Asp 385 390 395 400 Asn Thr Pro Val Val Asp Ala Ala Asp Val Pro Asn Leu Val Tyr Ile 405 410 415 Gln Ala Ile Ile Lys Glu Ser Met Arg Leu Tyr Pro Ala Ser Thr Leu 420 425 430 Met Glu Arg Met Thr Ser Asp Asp Cys Glu Val Gly Gly Phe His Val 435 440 445 Pro Ala Gly Thr Arg Leu Trp Val Asn Val Trp Lys Met Gln Arg Asp 450 455 460 Pro Arg Val Trp Asn Asp Pro Leu Val Phe Arg Pro Glu Arg Phe Leu 465 470 475 480 Ser Asn Asp Lys Gly Met Val Asp Val Lys Gly Gln Asn Tyr Glu Leu 485 490 495 Ile Pro Phe Gly Thr Gly Arg Arg Ile Cys Pro Gly Ala Ser Phe Ala 500 505 510 Leu Glu Val Leu His Leu Val Leu Thr Arg Leu Ile Leu Glu Phe Glu 515 520 525 Met Lys Ala Pro Glu Gly Glu Ile Asp Met Arg Ala Arg Pro Gly Phe 530 535 540 Phe Asn Asn Lys Val Val Pro Leu Asp Val Leu Leu Thr Pro Arg Thr 545 550 555 560 Leu Asp <210> 287 <211> 519 <212> PRT <213> Papaver bracteatum <400> 287 Met Asn Asn Tyr Ser Ser Ser Pro Ala Ser Ser Thr Glu Thr Ala Val 1 5 10 15 Leu Cys His Gln Arg Gln Gln Ser Cys Ala Leu Pro Ile Ser Gly Leu 20 25 30 Leu His Ile Phe Met Asn Lys Asn Gly Leu Ile His Val Thr Leu Gly 35 40 45 Asn Met Ala Asp Lys Tyr Gly Pro Ile Phe Ser Phe Pro Thr Gly Ser 50 55 60 His Arg Ile Leu Val Val Ser Ser Trp Glu Met Val Lys Glu Cys Phe 65 70 75 80 Thr Gly Asn Asn Asp Thr Phe Phe Ser Asn Arg Pro Ile Pro Leu Ala 85 90 95 Phe Lys Ile Ile Phe Tyr Ala Gly Gly Val Asp Ser Tyr Gly Leu Ala 100 105 110 Leu Val Pro Tyr Gly Lys Tyr Trp Arg Glu Leu Arg Lys Ile Cys Val 115 120 125 His Asn Leu Leu Ser Asn Gln Gln Leu Leu Lys Phe Arg His Leu Ile 130 135 140 Ile Ser Gln Val Asp Thr Ser Phe Asn Lys Leu Tyr Glu Leu Cys Lys 145 150 155 160 Asn Ser Glu Asp Asn Gln Gly Met Val Arg Met Asp Asp Trp Leu Ala 165 170 175 Gln Leu Ser Phe Ser Val Ile Gly Arg Ile Val Cys Gly Phe Gln Ser 180 185 190 Asp Pro Lys Thr Gly Ala Pro Ser Arg Val Glu Gln Phe Lys Glu Ala 195 200 205 Ile Asn Glu Ala Ser Tyr Phe Met Ser Thr Ser Pro Val Ser Asp Asn 210 215 220 Val Pro Met Leu Gly Trp Ile Asp Gln Leu Thr Gly Leu Thr Arg Asn 225 230 235 240 Met Thr His Cys Gly Lys Lys Leu Asp Leu Val Val Glu Ser Ile Ile 245 250 255 Asn Asp His Arg Gln Lys Arg Arg Phe Ser Arg Thr Lys Gly Gly Asp 260 265 270 Glu Lys Asp Asp Glu Gln Asp Asp Phe Ile Asp Ile Cys Leu Ser Ile 275 280 285 Met Glu Gln Pro Gln Leu Pro Gly Asn Asn Asn Pro Pro Lys Ile Pro 290 295 300 Ile Lys Ser Ile Val Leu Asp Met Ile Gly Gly Gly Thr Asp Thr Thr 305 310 315 320 Lys Leu Thr Thr Ile Trp Thr Leu Ser Leu Leu Leu Asn Asn Pro His 325 330 335 Val Leu Asp Lys Ala Lys Gln Glu Val Asp Ala His Phe Leu Thr Lys 340 345 350 Arg Arg Ser Thr Asn Asp Ala Ala Val Val Asp Phe Asp Asp Ile Arg 355 360 365 Asn Leu Val Tyr Ile Gln Ala Ile Ile Lys Glu Ser Met Arg Leu Tyr 370 375 380 Pro Ala Ser Pro Val Val Glu Arg Leu Ser Gly Glu Asp Cys Val Val 385 390 395 400 Gly Gly Phe His Val Pro Ala Gly Thr Arg Leu Trp Val Asn Val Trp 405 410 415 Lys Met Gln Arg Asp Pro Asn Val Trp Ala Asp Pro Met Val Phe Arg 420 425 430 Pro Glu Arg Phe Leu Ser Asp Glu Gln Lys Met Val Asp Val Arg Gly 435 440 445 Gln Asn Tyr Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro 450 455 460 Gly Val Ser Phe Ser Leu Asp Leu Met Gln Leu Val Leu Thr Arg Leu 465 470 475 480 Ile Leu Glu Phe Glu Met Lys Ser Pro Gly Gly Glu Val Asp Met Thr 485 490 495 Ala Thr Pro Gly Leu Met Ser Tyr Lys Val Leu Pro Leu Asp Val Leu 500 505 510 Leu Thr His Leu Ser Ala Ser 515 <210> 288 <211> 540 <212> PRT <213> Papaver bracteatum <400> 288 Met Glu Phe Leu Met Lys Leu Leu Leu Leu Leu Glu Pro Ile Thr Phe 1 5 10 15 Ser Ile Phe Leu Gly Ile Gly Ser Ile Val Leu Leu Tyr Asn Val Phe 20 25 30 Phe Leu Val Ile Asn Lys Lys Lys Lys Lys Lys Ala Pro Asn Ala Ser 35 40 45 Gly Ala Trp Pro Leu Ile Gly His Leu Asn Leu Phe Met Asn Asp Lys 50 55 60 Glu Ala Leu Tyr Lys Thr Leu Gly Thr Met Ala Asp Lys Tyr Gly Pro 65 70 75 80 Ala Phe Asn Val Arg Leu Gly Asn Gln Glu Ile Leu Val Val Ser Asn 85 90 95 Trp Lys Met Val Lys Glu Cys Phe Asn Thr Gln Asn Asp Lys Leu Phe 100 105 110 Ser Asn Arg Arg Thr Thr Leu Gly Val Lys Tyr Met Leu Asn Lys Lys 115 120 125 Thr Ser Val Ala Phe Ser Pro Tyr Gly Thr Tyr Trp Arg Glu Leu Arg 130 135 140 Lys Leu Thr Val Gln Gln Leu Leu Ser Lys Gln Arg Leu Asp Ser Trp 145 150 155 160 Lys His Leu Lys Ile Lys Glu Ile Asp Ala Ser Phe Gly Arg Leu Asn 165 170 175 Asp Leu Cys Ser Asn Asn Lys Gly Thr Gly Ala Ala Thr Pro Ile Arg 180 185 190 Met Asp Ser Trp Phe Ala Glu Leu Thr Phe Asn Val Phe Ala Arg Ile 195 200 205 Val Phe Gly Tyr Gln Ser Gly Glu Arg Leu Met Leu Ser Gly Asp Thr 210 215 220 Ala Ser Asn Gly Glu Arg Tyr Lys Lys Thr Leu Glu Glu Ala Phe Leu 225 230 235 240 Leu Met Ser Ser Phe Ala Val Ser Asp Val Phe Pro Cys Leu Glu Trp 245 250 255 Val Asp Arg Ser Arg Gly Leu Val Arg Ser Met Lys Arg Phe Gly Asp 260 265 270 Gln Leu Asn Ser Ile Ala Gly Cys Leu Ile Glu Glu His Arg Gln Lys 275 280 285 Arg Ser Gln Ser Val Ser Ala Ser Asn Ser Thr Asn Asp Lys Gly Val 290 295 300 Gly Asp Glu Gln Asp Phe Ile Asp Val Leu Leu Ser Val Ala Glu Leu 305 310 315 320 Ser Gln Ile Pro Gly Asp Asp Pro Asp Leu Val Ile Lys Ser Met Ile 325 330 335 Leu Glu Ala Leu Ala Gly Gly Ser Asp Thr Thr Thr Ser Thr Leu Thr 340 345 350 Trp Val Leu Ser Leu Leu Leu Asn His Pro Lys Val Leu Lys Thr Ala 355 360 365 Lys Glu Glu Ile Asp Met His Val Gly Arg Asn Arg Cys Val Glu Glu 370 375 380 Ser Asp Ile Pro Lys Leu Val Tyr Val Asn Ala Ile Ile Lys Glu Ser 385 390 395 400 Met Arg Leu Tyr Pro Asn Gly Ser Leu Val Asp Arg Leu Thr Leu Glu 405 410 415 Glu Cys Glu Val Gly Gly Phe His Val Pro Ala Glu Gly His Leu Phe 420 425 430 Val Asn Val Trp Lys Ile His Arg Asp Pro Ser Val Trp Glu Asn Pro 435 440 445 Leu Glu Phe Lys Pro Glu Arg Phe Leu Ser Asn Asp Cys Lys Val Asp 450 455 460 Met Asp Phe Ile Ser Gln Lys Tyr Glu Phe Ile Pro Phe Gly Ile Gly 465 470 475 480 Arg Arg Ile Cys Pro Gly Met Leu Ser Ala Leu Gln Val Met His Phe 485 490 495 Val Val Ala Arg Leu Ile His Gly Phe Asp Met Glu Ala Ala Ser Ala 500 505 510 Asp Gly Lys Val Asp Met Ala Glu Lys Pro Gly Met Thr Cys Tyr Lys 515 520 525 Met Thr Pro Leu Glu Val Met Leu Thr Ala Arg Gln 530 535 540 <210> 289 <211> 548 <212> PRT <213> Papaver bracteatum <400> 289 Met Tyr Pro Val Asn Gln Leu Gln Ser Gln Ala Ile Ala Val Leu Cys 1 5 10 15 Ala Val Ile Val Phe Ile Phe Tyr Leu Gly Arg Lys Leu Phe Asn Ser 20 25 30 Thr His Ile His Lys Pro Gly Lys Thr Ala Pro Glu Pro Ala Gly Ala 35 40 45 Trp Pro Ile Ile Gly His Leu His Leu Leu Gly Gly Ala Lys Leu Met 50 55 60 Tyr Arg Thr Leu Gly Ser Leu Ala Asp Glu Tyr Gly Pro Val Phe Met 65 70 75 80 Val Arg Leu Gly Met Arg Arg Val Leu Val Ile Ser Asn Thr Ala Ser 85 90 95 Ala Arg Glu Cys Phe Thr Thr Asn Asp Lys Val Phe Ala Thr Arg Pro 100 105 110 Thr Thr Val Ala Ile Lys Leu Leu Thr Tyr Asn His Thr Met Phe Gly 115 120 125 Phe Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys Ile Ala Thr 130 135 140 Thr Glu Leu Leu Ser Asp Arg Arg Leu Ala Met Leu Lys Asn Val Trp 145 150 155 160 Ile Ser Glu Ile Asn Leu Cys Ile Asn Glu Leu His Gln Leu Leu Met 165 170 175 Asp Lys Asn Ser Arg Lys Val Asp His His Gln His Val Tyr Asn Gln 180 185 190 Asn Met Asp Pro Ile Ser Val Glu Met Asn Gly Trp Phe Ala Asp Leu 195 200 205 Ser Phe Asn Val Val Ala Arg Met Ile Ala Gly Lys Arg Tyr Phe Gly 210 215 220 Lys Asn Thr Asp Gly Gly Glu Glu Glu Thr Arg Arg Tyr Arg Glu Ala 225 230 235 240 Met Asn Asp Phe Met His Leu Val Val Ile Leu Leu Val Ser Asp Ala 245 250 255 Val Pro Phe Leu Gly Gly Leu Asp Phe Gln Gly Tyr Lys Lys Arg Met 260 265 270 Lys Lys Thr Ala Lys Glu Ile Asp Tyr Phe Leu Gly Lys Trp Val Asp 275 280 285 Glu His Arg Gln Arg Leu Lys Tyr Lys Asn Ile Ser Lys Val Asp Gln 290 295 300 Gln Asp Asp Phe Ile Asp Val Leu Leu Leu Asn Leu Asn Asp Gln Pro 305 310 315 320 Ile Tyr Gly Arg Asp Thr Asp Thr Ile Ile Lys Ser Thr Cys Leu Ser 325 330 335 Leu Ile Ala Gly Gly Ser Asp Thr Thr Ala Val Thr Leu Thr Trp Ala 340 345 350 Leu Ser Leu Leu Leu Asn Asn Gln His Val Leu Arg Lys Ala Gln Asp 355 360 365 Glu Leu Asp Ile His Val Gly Arg Glu Arg Gln Leu Glu Glu Ser Asp 370 375 380 Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 385 390 395 400 Leu Tyr Pro Ala Ala Pro Leu Ser Ala Pro Arg Met Ala Met Glu Asp 405 410 415 Cys Thr Val Ala Gly Phe Gln Val Ser Lys Gly Thr Gln Leu Met Leu 420 425 430 Asn Val Trp Lys Leu His Arg Asp Pro His Phe Trp Gly Pro Asp Pro 435 440 445 Leu Glu Phe Arg Pro Glu Arg Phe Leu Thr Ala Asp Gly Ser Ser Thr 450 455 460 Gly Ser Gly His Cys Ile Asp Ile Asp Val Lys Gly Arg His Tyr Glu 465 470 475 480 Leu Leu Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Val Ser Phe 485 490 495 Ala Met Gln Val Val His Leu Thr Leu Ala Thr Leu Leu His Gly Phe 500 505 510 His Leu Ser Thr Pro Thr Asp Gly Pro Val Asp Met Thr Glu Thr Ser 515 520 525 Gly Leu Ser Cys Pro Lys Ala Thr Pro Leu Glu Val Leu Leu Thr Pro 530 535 540 Arg Leu Pro Cys 545 <210> 290 <211> 567 <212> PRT <213> Papaver bracteatum <400> 290 Met Asp Val Ala Ile Ile Val Asp His His Tyr Leu Gln Pro Phe Val 1 5 10 15 Ser Ile Ala Gly Leu Leu Ala Leu Leu Ser Phe Phe Tyr Cys Ile Trp 20 25 30 Val Phe Ile Ile Arg Pro Arg Ile Ile Lys Ser Asn Leu Asp Glu Arg 35 40 45 Lys Leu Ser Pro Ser Ser Pro Pro Glu Val Ala Gly Ala Trp Pro Ile 50 55 60 Val Gly His Leu Pro Gln Leu Ile Gly Ser Thr Pro Leu Phe Lys Ile 65 70 75 80 Leu Ala Asp Met Ser Asn Lys Tyr Gly Pro Ile Phe Met Val Arg Phe 85 90 95 Gly Met Tyr Pro Thr Leu Val Val Ser Ser Trp Glu Met Ser Lys Glu 100 105 110 Cys Phe Thr Thr Asn Asp Arg Leu Phe Ala Thr Arg Pro Pro Ser Ala 115 120 125 Ala Gly Lys Tyr Leu Thr Lys Ala Leu Phe Ala Phe Ser Val Tyr Gly 130 135 140 Pro Tyr Trp Arg Glu Ile Arg Lys Ile Ser Thr Ile His Leu Leu Ser 145 150 155 160 Leu Arg Arg Leu Glu Leu Leu Lys His Gly Arg Tyr Leu Glu Ile Asp 165 170 175 Lys Cys Met Lys Arg Leu Phe Glu Tyr Trp Met Glu His His Lys Asn 180 185 190 Ile Ile Ser Thr Thr Ser Ser Val Lys Val Asn Met Ser Gln Val Phe 195 200 205 Ala Glu Leu Ser Leu Asn Val Val Leu Lys Ile Ile Val Gly Lys Thr 210 215 220 Leu Phe Ile Lys Asn Gly Asn Glu Asp Tyr Thr Lys Glu Glu Glu Glu 225 230 235 240 Gly Gln Lys Leu His Lys Thr Ile Leu Lys Phe Met Glu Leu Ala Gly 245 250 255 Val Ser Val Ala Ser Asp Val Leu Pro Phe Leu Gly Trp Leu Asp Val 260 265 270 Asp Gly Gln Lys Lys Gln Met Lys Arg Val Tyr Lys Glu Met Asn Leu 275 280 285 Ile Ala Ser Lys Trp Leu Gly Glu His Arg Glu Arg Lys Arg Leu Gln 290 295 300 Ile Ile Gln Lys Arg Gly Ala Ala Arg Gly Ser Asn Tyr Asp Asp Gly 305 310 315 320 Asn Asp Phe Met Asp Val Leu Met Ser Ile Leu Asp Glu Glu Asn Asp 325 330 335 Asp Leu Phe Phe Gly Tyr Ser Arg Asp Thr Val Ile Lys Ser Thr Cys 340 345 350 Leu Gln Leu Ile Val Ala Ala Ser Asp Thr Thr Ser Leu Ala Met Thr 355 360 365 Trp Ala Leu Ser Leu Leu Leu Thr Asn Pro Asn Val Leu Gln Lys Ala 370 375 380 Gln Asp Glu Leu Asp Thr Lys Val Gly Arg Asp Arg Ile Ile Glu Glu 385 390 395 400 His Asp Ile Glu Cys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr 405 410 415 Leu Arg Leu Tyr Pro Pro Ala Pro Leu Ser Leu Pro His Glu Ala Met 420 425 430 Glu Asp Cys Thr Val Gly Gly Tyr Gln Val Lys Ala Gly Thr Arg Leu 435 440 445 Val Val Asn Leu Trp Lys Leu Gln Arg Asp Pro Arg Val Trp Ser Asn 450 455 460 Pro Leu Glu Phe Lys Pro Glu Arg Phe Leu Pro Gln Ser Asp Gly Gly 465 470 475 480 Phe Gly Gly Glu Glu Ala Arg Met Asp Phe Arg Gly Gln His Phe Glu 485 490 495 Tyr Thr Pro Phe Gly Ser Gly Arg Arg Ile Cys Pro Gly Ile Asp Phe 500 505 510 Phe Leu Gln Thr Val His Met Ala Leu Ala Arg Leu Leu Gln Ala Phe 515 520 525 Asp Phe Asn Thr Ala Gly Gly Leu Val Ile Asp Met Val Glu Gly Pro 530 535 540 Gly Leu Thr Met Pro Lys Val Thr Pro Leu Glu Val His Leu Asn Pro 545 550 555 560 Arg Leu Pro Val Thr Leu Tyr 565 <210> 291 <211> 558 <212> PRT <213> Papaver bracteatum <400> 291 Met Gln Val Asp Trp Pro Asn Ile Leu Gln Lys Tyr Tyr Pro Ile Ile 1 5 10 15 Thr Cys Ser Leu Leu Thr Leu Leu Ser Phe Tyr Tyr Ile Trp Val Ser 20 25 30 Ile Thr Lys Pro Ser Arg Asn Ser Lys Thr Lys Leu Pro Pro Pro Glu 35 40 45 Val Ala Gly Ser Trp Pro Ile Val Gly His Leu Pro Gln Leu Val Gly 50 55 60 Ser Thr Pro Leu Phe Lys Ile Leu Ala Asn Met Ser Asp Lys Tyr Gly 65 70 75 80 Pro Ile Phe Met Val Arg Phe Gly Met His Pro Thr Leu Val Val Ser 85 90 95 Ser Trp Glu Met Ser Lys Glu Cys Phe Thr Thr Asn Asp Lys Phe Leu 100 105 110 Ala Ser Arg Pro Pro Ser Ala Ser Ala Lys Tyr Leu Gly Tyr Asp Asn 115 120 125 Ala Met Phe Val Phe Ser Asp Tyr Gly Pro Tyr Trp Arg Glu Ile Arg 130 135 140 Lys Ile Ser Thr Leu Gln Leu Leu Thr His Lys Arg Leu Asp Ser Leu 145 150 155 160 Lys Asn Ile Pro Tyr Leu Glu Ile Asn Ser Cys Val Lys Thr Leu Tyr 165 170 175 Thr Arg Trp Ala Lys Thr Gln Ser Gln Ile Lys Gln Asn Val Gly Gly 180 185 190 Ala Ala Asp Asp Phe Val Lys Val Asp Met Thr Glu Met Phe Gly His 195 200 205 Leu Asn Leu Asn Val Val Leu Arg Leu Val Val Gly Lys Pro Ile Phe 210 215 220 Ile Gln Lys Asp Asn Ala Asp Glu Asp Tyr Thr Lys Asp Gly His Asn 225 230 235 240 Lys Glu Glu Leu Gly Gln Lys Leu His Lys Thr Ile Ile Glu Phe Phe 245 250 255 Glu Leu Ala Gly Ala Ser Val Ala Ser Asp Val Leu Pro Tyr Leu Gly 260 265 270 Trp Leu Asp Val Asp Gly Gln Lys Lys Arg Met Lys Lys Ile Ala Met 275 280 285 Glu Met Asp Leu Phe Ala Gln Lys Trp Leu Glu Glu His Arg Gln Lys 290 295 300 Gly Ile Asn His Asp Asn Glu Asn Asp Phe Met Ala Val Leu Ile Ser 305 310 315 320 Val Leu Gly Glu Gly Lys Asp Asp His Ile Phe Gly Tyr Ser Arg Asp 325 330 335 Thr Val Ile Lys Ala Thr Cys Leu Thr Leu Ile Val Ala Ala Thr Asp 340 345 350 Thr Thr Leu Val Ser Leu Thr Trp Ala Leu Ser Leu Leu Leu Thr Asn 355 360 365 Pro Arg Val Leu Ser Lys Ala Gln Asp Glu Leu Asp Thr Val Val Gly 370 375 380 Lys Glu Arg Asn Val Glu Asp Arg Asp Val Asn His Leu Val Tyr Leu 385 390 395 400 Gln Ala Val Ile Lys Glu Thr Leu Arg Leu Tyr Pro Pro Ser Pro Leu 405 410 415 Ala Val Pro His Glu Ala Ile Glu Asn Cys Asn Val Gly Gly Tyr Glu 420 425 430 Val Lys Ala Arg Thr Arg Leu Leu Val Asn Leu Trp Lys Ile His Arg 435 440 445 Asp Pro Arg Val Trp Ser Asn Pro Leu Glu Phe Lys Pro Glu Arg Phe 450 455 460 Leu Pro Lys Leu Asp Gly Gly Thr Gly Glu Ala Ser Lys Leu Asp Phe 465 470 475 480 Lys Gly Gln Asp Phe Val Tyr Thr Pro Phe Gly Ser Gly Arg Arg Met 485 490 495 Cys Pro Gly Ile Asn Phe Ala Ser Gln Thr Leu His Met Thr Leu Ala 500 505 510 Arg Leu Leu His Ala Phe Asp Phe Asp Ile Glu Ser Asn Gly Leu Val 515 520 525 Ile Asp Met Thr Glu Gly Ser Gly Leu Thr Met Pro Lys Val Thr Pro 530 535 540 Leu Gln Val His Leu Arg Pro Arg Leu Pro Ala Thr Leu Tyr 545 550 555 <210> 292 <211> 571 <212> PRT <213> Papaver bracteatum <400> 292 Met Met Asp Leu Ala Met Phe Ile Asp Gln Tyr Phe Ser Leu Ala Lys 1 5 10 15 Ile Ala Gly Leu Leu Ala Leu Leu Ser Phe Phe Tyr Tyr Leu Trp Ile 20 25 30 Ser Thr Leu Trp Ser Pro Arg Asn Pro Lys Leu Ser Ser Val Ser Pro 35 40 45 Pro Glu Val Ala Gly Ala Trp Pro Ile Leu Gly His Leu Pro Gln Leu 50 55 60 Leu Gly Ser Arg Pro Leu Phe Lys Ile Leu Ala Asp Met Ser Asp Asn 65 70 75 80 Tyr Gly Pro Ile Phe Met Val Arg Phe Gly Met His Pro Thr Leu Val 85 90 95 Val Ser Ser Trp Glu Met Ala Lys Glu Cys Phe Thr Thr Asn Asp Arg 100 105 110 Phe Leu Ala Gly Arg Pro Ser Gly Ala Ala Asn Lys Tyr Leu Thr Phe 115 120 125 Ala Leu Phe Gly Phe Ser Thr Tyr Gly Pro Tyr Trp Arg Glu Ile Arg 130 135 140 Lys Ile Ala Thr Leu His Leu Leu Ser His Arg Arg Leu Glu Leu Leu 145 150 155 160 Lys His Val Pro Asp Leu Glu Val Thr Asn Cys Met Lys His Leu His 165 170 175 Arg Arg Trp Ile Asp Ser Gln Asn Gln Ile Lys Gln Asn Asp Ala Ala 180 185 190 Ala Gly Ser Val Lys Val Asp Met Gly Arg Val Phe Gly Glu Leu Thr 195 200 205 Leu Asn Val Val Leu Lys Leu Val Ala Gly Lys Ser Ile Phe Phe Lys 210 215 220 Asn Asp Asn Thr Arg Gln Tyr Asp Ser Lys Asp Gly His Asn Lys Glu 225 230 235 240 Glu Glu Glu Gly Lys Lys Leu His Lys Thr Ile Ile Asp Phe Tyr Ser 245 250 255 Leu Ala Gly Ala Ser Val Ala Ser Asp Val Leu Pro Phe Leu Gly Trp 260 265 270 Leu Asp Val Asp Gly Gln Lys Lys Arg Met Lys Arg Val Ala Lys Asp 275 280 285 Met Asp Phe Ile Ala Ala Lys Trp Leu Glu Glu His Arg His Gln Lys 290 295 300 Arg Gln Thr Val Leu Ser Ser Ser Ala Thr Leu Gly Ser Ser Asn His 305 310 315 320 Asp Asp Ala Lys Asp Phe Met Asp Val Leu Met Ser Ile Leu Asp Gly 325 330 335 Glu Asn Asp Asp Leu Phe Phe Gly Tyr Ser Arg Asp Thr Val Ile Lys 340 345 350 Thr Thr Cys Leu Gln Leu Ile Ala Ala Ala Ala Asp Thr Thr Ser Val 355 360 365 Thr Met Thr Trp Ala Leu Ala Leu Leu Ile Thr Asn Pro Thr Ile Leu 370 375 380 Arg Lys Ala Gln Asp Glu Leu Asp Thr Lys Val Gly Lys Asp Arg Asn 385 390 395 400 Ile Glu Glu Arg Asp Ile Asn Asp Leu Val Tyr Leu Gln Ala Ile Val 405 410 415 Lys Glu Thr Leu Arg Met Tyr Pro Ala Gly Pro Leu Asn Val Pro His 420 425 430 Glu Ala Ile Ala Asp Cys Asn Ile Gly Gly Tyr Glu Val Arg Ala Gly 435 440 445 Thr Arg Leu Leu Val Asn Leu Trp Lys Met His Arg Asp Pro Arg Val 450 455 460 Trp Ser Asn Pro Ser Glu Phe Lys Pro Glu Arg Phe Leu Pro Gln Leu 465 470 475 480 Asp Gly Gly Ser Gly Gly Glu Ala Ala Asn Leu Asp Phe Arg Gly Gln 485 490 495 Asp Phe Glu Tyr Leu Pro Phe Ser Ala Gly Arg Arg Met Cys Pro Gly 500 505 510 Ile Asp Phe Ser Leu Gln Thr Leu His Met Thr Leu Ala Arg Leu Leu 515 520 525 His Gly Phe Asp Phe Asn Asn Asp Ser Ala Gly Ile Ile Ile Asp Met 530 535 540 Glu Glu Gly Ser Gly Leu Thr Met Pro Lys Leu Thr Pro Leu Glu Ile 545 550 555 560 Tyr Leu Cys Pro Arg Leu Pro Ala Lys Leu Tyr 565 570 <210> 293 <211> 543 <212> PRT <213> Sanguinaria canadensis <400> 293 Met Glu Phe Leu Ser Leu Gln Phe Gln Pro Ile Thr Ile Phe Ala Leu 1 5 10 15 Leu Leu Ala Thr Pro Ile Phe Leu Tyr Asn Leu Trp Asn Tyr Gly Arg 20 25 30 Lys Leu Asn Asn Lys Asn Lys Lys Ser Arg Lys Pro Pro Ala Pro Glu 35 40 45 Ala Ser Gly Gly Trp Pro Ile Met Gly His Phe His Leu Phe Asn Gly 50 55 60 Thr Glu Val Thr His Arg Ala Leu Gly Ser Met Ala Asp Lys Tyr Gly 65 70 75 80 Pro Ala Phe Asn Ile Arg Ile Gly Ser His Pro Thr Leu Val Val Ser 85 90 95 Ser Trp Glu Ile Ala Lys Glu Cys Phe Thr Thr Asn Asp Arg Leu Phe 100 105 110 Ser Asn Arg Pro Gly Ser Leu Ala Ile Lys Leu Met Phe Tyr Asp Ala 115 120 125 Asp Ser Val Gly Tyr Ala Pro Tyr Gly Thr Tyr Trp Arg Glu Leu Arg 130 135 140 Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn His Arg Leu Glu Thr Leu 145 150 155 160 Lys His Leu Arg Thr Ser Glu Val Glu Ser Cys Phe Lys Gln Leu His 165 170 175 Asp Leu Trp Arg Met Asn Asn Lys Lys Gly Gly Ser Asp Asp Gly Ser 180 185 190 Asp Phe Ala Leu Val Arg Ile Asp Asn Trp Phe Gly Asp Leu Thr Phe 195 200 205 Asn Val Val Ala Arg Ile Val Ala Gly Lys Lys Asn Phe Ala Gly Gly 210 215 220 Ala Ala Ser Gly Asp Ala Gly Ala Gln Arg Tyr Lys Glu Ala Met Asp 225 230 235 240 Glu Ala Phe Arg Leu Met Thr Arg Phe Ala Phe Ser Asp Val Val Pro 245 250 255 Ser Leu Gly Trp Leu Asp Lys Leu Thr Gly Leu Val Gly Gly Met Lys 260 265 270 Arg Cys Gly Ser Glu Ile Asp Ser Ile Val Ala Ser Trp Val Asp Glu 275 280 285 His Arg Leu Lys Arg Ile Ser Gly Lys Glu Gly Asp Asp Leu Glu Gln 290 295 300 Asp Phe Ile Asp Val Cys Leu Glu Ile Leu Glu His Ser Thr Leu Pro 305 310 315 320 Gly Asp Asp Pro Glu Val Val Ile Lys Ser Thr Cys Leu Asp Met Ile 325 330 335 Leu Gly Gly Ser Asp Thr Thr Thr Val Thr Leu Thr Trp Ala Leu Ser 340 345 350 Leu Leu Leu Asp His Pro His Val Trp Lys Arg Ala Lys Glu Glu Val 355 360 365 Asp Ala His Val Gly Lys Glu Arg Gln Val Glu Asp Ser Asp Ile Pro 370 375 380 Asn Leu Val Phe Ile Gln Ala Ile Val Lys Glu Thr Met Arg Leu Tyr 385 390 395 400 Pro Ala Gly Pro Leu Ile Glu Arg Arg Thr Met Asp Asp Cys Glu Val 405 410 415 Gly Gly Phe His Ile Pro Gly Gly Thr Arg Leu Ile Val Asn Leu Trp 420 425 430 Lys Ile Gln Arg Asp Gly Ser Val Tyr Lys Glu Asp Pro Leu Glu Phe 435 440 445 Arg Pro Glu Arg Phe Leu Thr Ser Asn Ala Glu Val Asp Leu Lys Gly 450 455 460 Gln Asn Tyr Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Pro 465 470 475 480 Gly Val Ser Phe Ala Val Gln Leu Met His Leu Val Leu Ala Arg Leu 485 490 495 Ile His Asp Phe Glu Ile Thr Met Pro Met Gly Ala Lys Val Asp Met 500 505 510 Thr Glu Ser Gly Gly Leu Ile Ser His Lys Val Thr Pro Leu Glu Val 515 520 525 Leu Leu Lys Pro Arg Leu Pro Ser Leu Gln Gln Ala Ala Leu Tyr 530 535 540 <210> 294 <211> 525 <212> PRT <213> Sanguinaria canadensis <400> 294 Met Glu Tyr Ser Ser Val Leu Leu His Trp Phe Pro Ala Ser Met Ala 1 5 10 15 Ala Leu Leu Ala Leu Val Phe Leu Tyr Asn Leu Ile Phe Asn Ser Pro 20 25 30 Lys Thr Gly Asn Lys Lys Ile Ile Arg Arg Ala Pro Lys Ala Ala Gly 35 40 45 Ala Trp Pro Val Leu Gly His Leu His Leu Phe Gly Ser Gly Glu Leu 50 55 60 Pro His Lys Met Leu Ala Ala Met Ala Glu Lys Tyr Gly Pro Ala Phe 65 70 75 80 Thr Met Lys Phe Gly Lys His Thr Thr Leu Val Val Ser Asp Thr Arg 85 90 95 Val Val Lys Glu Cys Phe Thr Thr Asn Asp Thr Leu Phe Ala Asn Arg 100 105 110 Pro Ser Thr Lys Ala Phe Asp Leu Met Thr Tyr Ala Asn Asp Ser Val 115 120 125 Ala Phe Thr Pro Tyr Ser Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser 130 135 140 Thr Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ser Ile Lys Glu Val 145 150 155 160 Arg Val Ser Glu Val Ser Val Cys Phe Arg Glu Leu Tyr Asp Ile Cys 165 170 175 Lys Asn Asp Gly Gly Gly Ala Pro Pro Val Leu Val Asp Met Lys Lys 180 185 190 Trp Phe Glu Glu Val Ser Asn Asn Ile Val Met Arg Val Ile Val Gly 195 200 205 Lys Gln Asn Phe Gly Ser Lys Ile Val Cys Gly Glu Glu Glu Ala Val 210 215 220 Asn Tyr Lys Asn Val Met Asp Glu Leu Leu Arg Leu Ala Ser Val Ser 225 230 235 240 Met Leu Ser Asp Val Ala Pro Leu Leu Gly Trp Leu Asp Met Phe Gln 245 250 255 Gly His Met Ser Ala Met Arg Arg Asn Gly Lys Lys Leu Asp Thr Ile 260 265 270 Leu Glu Arg Trp Leu Glu Glu His Arg Lys Lys Lys Ser Glu Asp Glu 275 280 285 Gln Asp Phe Met Asp Val Met Leu Ser Ile Val Glu Glu Thr Lys Leu 290 295 300 Ser Gly Arg Asp Ala Asp Thr Val Ile Lys Ala Thr Cys Leu Ala Met 305 310 315 320 Ile Met Gly Gly Thr Asp Thr Thr Ala Val Ser Leu Thr Trp Ile Val 325 330 335 Ser Leu Leu Met Asn Asn Arg Tyr Ala Leu Lys Lys Ala Arg Glu Glu 340 345 350 Leu Asp Ala Leu Val Gly Lys Asp Arg Gln Val Glu Asp Ser Asp Leu 355 360 365 Lys Asn Leu Val Tyr Leu Asn Ala Val Val Lys Glu Thr Met Arg Leu 370 375 380 Tyr Pro Leu Gly Thr Leu Leu Glu Arg Asp Thr Lys Glu Asp Cys Glu 385 390 395 400 Val Gly Gly Phe His Val Glu Gly Gly Thr Arg Leu Leu Val Asn Val 405 410 415 Trp Lys Leu Gln Arg Asp Pro Asn Val Trp Asn Asp Pro Leu Glu Phe 420 425 430 Arg Pro Glu Arg Phe Leu Thr Glu Asn Ala Asp Ile Asp Val Gly Gly 435 440 445 Gln His Phe Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys Pro 450 455 460 Gly Val Ser Phe Ala Leu Gln Phe Met His Leu Val Leu Ala Arg Leu 465 470 475 480 Ile His Gly Tyr Glu Leu Gly Thr Gln Asn Asp Glu Asp Val Asp Leu 485 490 495 Thr Glu Ser Thr Glu Gly His Val Asn His Lys Ala Ser Pro Leu Asp 500 505 510 Leu Leu Ile Thr Pro Arg Leu His Pro Lys Leu Tyr Glu 515 520 525 <210> 295 <211> 527 <212> PRT <213> Sanguinaria canadensis <400> 295 Met Asp Tyr Ser Ser His Leu Asn Gln Phe Tyr Gly Val Ala Pro Tyr 1 5 10 15 Met Ala Ala Leu Leu Ile Ala Leu Val Phe Leu Tyr Asn Ile Leu Trp 20 25 30 Ala Ser Pro Lys Thr Thr Asn Lys Lys Pro Ile Met Ala Ala Gly Ala 35 40 45 Trp Pro Ile Leu Gly His Leu His Leu Phe Lys Asp Gly Glu Leu Pro 50 55 60 His His Met Leu Lys Ser Met Ser Asp Lys Tyr Gly Pro Ala Phe Leu 65 70 75 80 Met Lys Phe Gly Gln His Arg Thr Leu Val Val Ser Asn Tyr Arg Met 85 90 95 Val Lys Glu Cys Phe Thr Thr Asn Asp Thr His Phe Cys Asn Arg Pro 100 105 110 Ser Thr Thr Ala Phe Asp Val Met Thr Tyr Ala Asn Asp Ser Val Ala 115 120 125 Phe Thr Ala Tyr Ser Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn Asn Arg Leu Gln Ala Ile Lys Asn Leu Arg 145 150 155 160 Glu Ala Glu Val Asn Val Ser Phe Arg Gly Leu Tyr Asp Leu Trp Lys 165 170 175 Asn Asn Ser Asp Arg Ala Ala Ser Val Leu Val Asp Met Lys Lys Trp 180 185 190 Phe Glu Glu Val Ser Asn Asn Val Val Ile Arg Val Ile Val Gly Lys 195 200 205 His Asn Phe Gly Thr Lys Ile Val Lys Gly Glu Lys Glu Ala Val Glu 210 215 220 Tyr Lys Thr Ile Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Leu 225 230 235 240 Leu Ser Asp Phe Ala Pro Ile Leu Gly Trp Val Asp Phe Phe Gln Gly 245 250 255 Asn Val Arg Lys Met Lys Arg Asn Gly Lys Lys Leu Asp Val Val Leu 260 265 270 Gln Arg Trp Leu Glu Thr His Arg Arg Lys Lys Asn Ser Pro Glu Asp 275 280 285 Glu Gln Asp Phe Met Asp Val Met Leu Ser Ile Val Glu Glu Gly Asp 290 295 300 Lys Leu Ser Gly His His Ala Asp Thr Val Ile Lys Ala Thr Cys Leu 305 310 315 320 Ala Met Ile Met Gly Gly Thr Asp Thr Ser Ala Val Ser Leu Thr Trp 325 330 335 Ile Val Ser Leu Leu Met Asn Asn Arg His Ala Leu Lys Lys Ala Arg 340 345 350 Glu Glu Leu Asp Met Tyr Val Gly Lys Asp Arg Gln Val Glu Asp Ser 355 360 365 Asp Leu Lys Asp Leu Val Tyr Leu His Ala Ile Val Lys Glu Thr Met 370 375 380 Arg Leu Tyr Pro Leu Gly Thr Leu Leu Glu Arg Glu Thr Lys Glu Asp 385 390 395 400 Cys Lys Val Gly Gly Phe His Val Glu Ala Gly Thr Arg Leu Leu Val 405 410 415 Asn Ile Trp Met Val Gln Arg Asp Pro Thr Val Trp Ser Asp Pro Thr 420 425 430 Lys Phe Ile Pro Glu Arg Phe Leu Thr Glu Lys Ala Asp Ile Asp Val 435 440 445 Gly Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Val 450 455 460 Cys Pro Ala Val Ser Phe Ala Leu Gln Phe Leu His Leu Ile Leu Ala 465 470 475 480 Arg Leu Ile His Gly Tyr Glu Leu Gly Thr Pro Asn Asp Ala Asp Val 485 490 495 Asp Leu Thr Glu Ser Pro Glu Gly His Val Asn His Lys Ala Ser Pro 500 505 510 Leu Glu Leu Leu Leu Thr Pro Arg Leu Asp Pro Lys Leu Tyr Ile 515 520 525 <210> 296 <211> 535 <212> PRT <213> Sanguinaria canadensis <400> 296 Met Asn Ser Ile Ala Val Val Gly Val Leu Leu Phe Phe Leu Tyr Tyr 1 5 10 15 Leu Trp Arg Thr Leu Phe Thr Thr His Lys Ala Ala Pro Pro Pro Gln 20 25 30 Pro Ala Gly Ala Arg Pro Ile Ile Gly His Leu His Leu Leu Gly Gly 35 40 45 Ala Asn Gln Leu Val His Gln Thr Leu Gly Ser Met Ala Asp Lys Tyr 50 55 60 Gly Ser Ile Phe Met Ile Arg Leu Gly Met Arg Arg Val Leu Val Val 65 70 75 80 Ser Ser Ser Glu Leu Ala Lys Glu Cys Phe Thr Thr Asn Asp Lys Val 85 90 95 Phe Leu Asn Arg Pro Ser Ser Leu Ala Leu Lys Leu Met Ala Tyr Asn 100 105 110 Asn Ala Ile Phe Gly Phe Ala Pro Phe Gly Pro Tyr Trp Arg Lys Met 115 120 125 Arg Lys Ile Ala Val Thr Glu Leu Leu Ser Asn Arg Arg Leu Glu Ile 130 135 140 Leu Lys Asn Ile Arg Ile Ser Glu Ile Asn Met Ser Ile Lys Glu Leu 145 150 155 160 His Gln Leu Trp Val Asp Met Asn Ser Gly Asp Asp Gln Val Gly Gly 165 170 175 Pro Pro Ile Val Val Glu Met Arg Lys Trp Phe Gly Asp Leu Ser Phe 180 185 190 Asn Val Val Gly Arg Met Ile Ala Gly Lys Arg Tyr Phe Gly Arg Asn 195 200 205 Cys Asp Cys Asp Glu Glu Glu Thr Arg Arg Tyr Gln Lys Ala Met Gly 210 215 220 Asp Phe Met His Leu Val Gly Val Phe Val Val Ser Asp Ala Ile Pro 225 230 235 240 Leu Leu Gly Phe Leu Asp Leu Gln Gly Tyr Glu Lys Lys Met Lys Arg 245 250 255 Thr Ala Met Asp Ile Asp Cys Val Leu Gly Arg Trp Val Asp Glu His 260 265 270 Arg Arg Arg Arg Gln Asp His Asp Glu Lys Arg Ile Met Val Ser Asp 275 280 285 Asp Asp His Gln Gly Gln Asp Phe Ile Asp Val Met Leu Ser Ile Leu 290 295 300 Asn Asp Asp Asp Gln Phe Tyr Gly Tyr Asp Ala Asp Thr Val Ile Lys 305 310 315 320 Ser Thr Cys Leu Ser Leu Ile Ala Gly Gly His Glu Thr Thr Ala Val 325 330 335 Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His Val Leu 340 345 350 Gln Asn Ala Gln Asp Glu Leu Asp Ile His Ile Ser Lys Glu Arg Gln 355 360 365 Val Asp Glu Ser Asp Ile Lys Asn Leu Lys Tyr Leu Gln Ala Ile Val 370 375 380 Lys Glu Ala Leu Arg Leu Tyr Pro Ala Leu Pro Leu Ser Ala Leu Arg 385 390 395 400 Leu Ala Met Glu Asp Cys Thr Ile Ala Gly Phe His Val Pro Lys Gly 405 410 415 Thr Glu Leu Met Leu Asn Leu Trp Lys Leu His Arg Asp Pro His Val 420 425 430 Trp Ser Asp Pro Leu Glu Phe Lys Pro Glu Arg Phe Leu Thr Asn Ser 435 440 445 Thr His Glu Glu Val Asp Val Gly Gly Gln His Tyr Glu Leu Leu Pro 450 455 460 Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Val Ser Phe Ala Leu Gln 465 470 475 480 Val Leu His Leu Thr Leu Ala Arg Leu Leu His Gly Phe His Leu Ser 485 490 495 Thr Pro Ser Gly Val Pro Val Asp Met Thr Gln Ser Phe Gly Leu Ser 500 505 510 Cys Pro Lys Ala Thr Pro Leu Asp Val Leu Leu Thr Pro Arg Leu Pro 515 520 525 Ser Lys Leu Tyr Tyr Val Ala 530 535 <210> 297 <211> 601 <212> PRT <213> Sanguinaria canadensis <400> 297 Met Ile Ser Thr Thr Pro Asp Val Leu Ala Thr Ser Ser Tyr Ser Ser 1 5 10 15 Ser Asn Lys His Met Asp Ser Asp His Leu Tyr Arg Phe Ser Thr Ser 20 25 30 Thr Ser Ile Val Ile Gly Leu Phe Ile Leu Leu Phe Phe Leu Val Leu 35 40 45 Leu Trp Lys Pro Lys Lys Gln Ser Ala Thr Ser Lys Thr Asn Lys Pro 50 55 60 Thr Arg Glu Gln Arg Ala Pro Glu Pro Ser Ser Ser Trp Pro Ile Ile 65 70 75 80 Gly His Leu Arg Leu Phe Gly Gly Pro Asn Ser Leu Pro His Ile Thr 85 90 95 Leu Gly Ala Met Ala Asp Lys Tyr Gly Pro Val Phe Thr Ile Arg Met 100 105 110 Gly Ser Arg Pro Val Leu Val Val Ser Ser Trp Glu Thr Ala Lys Glu 115 120 125 Cys Phe Thr Thr Asn Asp Arg Val Phe Ala Ser Arg Pro Arg Thr Ile 130 135 140 Ala Ile Lys His Met Cys Tyr Glu His Val Met Leu Gly Phe Ala Pro 145 150 155 160 Tyr Gly Ser Tyr Trp Arg Glu Leu Arg Lys Ile Val Asn Arg Glu Leu 165 170 175 Leu Ser Asn Asn Arg Leu Glu Leu Leu Lys His Val Trp Gly Ser Glu 180 185 190 Ile Asn Thr Ser Ile Lys Glu Leu Tyr Glu Leu Trp Leu Val Asn Lys 195 200 205 Asn Asn Ile Glu Ala Glu Gly Glu Asn Glu Gly Thr Ser Asp Arg Val 210 215 220 Leu Val Glu Met Glu Arg Trp Phe Ala Asp Leu Thr Leu Asn Met Ser 225 230 235 240 Ile Lys Ile Val Val Gly Lys Arg Tyr Pro Ser Gly Val Thr Ala Gly 245 250 255 Gly Ser Ser Gly Cys Asp Asp Asp Glu Ala Gln Arg Cys Arg Lys Ala 260 265 270 Leu Arg Asp Phe Phe Lys Leu Val Gly Leu Phe Leu Val Ser Asp Ala 275 280 285 Ile Pro Phe Leu Arg Trp Leu Asp Leu Gly Gly Tyr Glu Arg Glu Met 290 295 300 Lys Thr Thr Ala Arg Glu Leu Asp Cys Leu Met Glu Glu Trp Leu Glu 305 310 315 320 Glu His Lys Met Lys Arg Arg Leu Leu Ile Ser Pro Ser Ala Gly Asp 325 330 335 His His Glu Ala Ala Glu Ile Met Ile Asn Lys Gln Lys Glu Ala Glu 340 345 350 Leu Gln Glu Tyr Asp Phe Met Asp Val Met Leu Ser Ile Leu Asp Asp 355 360 365 Asp Ile Glu Gly Lys Asn Leu Ser Asp Tyr Asp Val Asn Ala Asp Thr 370 375 380 Ile Asn Lys Ser Thr Cys Leu Asn Leu Ile Leu Gly Gly Ser Asp Thr 385 390 395 400 Thr Met Val Thr Leu Thr Trp Ala Leu Ala Leu Leu Leu Asn Asn Pro 405 410 415 His Val Leu Gln Lys Ala Gln Asp Glu Leu Asp Ile His Val Gly Ser 420 425 430 Lys Arg Gln Val Asp Asp Ser Asp Met Lys Asn Leu Ile Tyr Leu Gln 435 440 445 Ala Ile Val Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ser 450 455 460 Val Pro His Glu Ser Thr Glu Asp Cys Thr Val Ala Gly Tyr His Val 465 470 475 480 Pro Ala Gly Thr Arg Leu Ile Val Asn Thr Trp Lys Ile Gln Arg Asp 485 490 495 Pro Arg Val Trp Ser Asn Pro Ser Glu Phe Lys Pro Glu Arg Phe Leu 500 505 510 Thr Asp Ser His Val Asp Ile Asp Val Arg Gly Arg Asn Phe Glu Leu 515 520 525 Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Thr Ser Phe Ala 530 535 540 Leu Gln Val Val His Leu Val Leu Ala Arg Leu Ile His Gly Phe Glu 545 550 555 560 Phe Lys Thr Pro Ser Glu Ala Pro Ile Asp Met Thr Glu Ser Val Gly 565 570 575 Leu Ser Asn Phe Lys Ala Thr Pro Leu Asp Val Leu Leu Thr Pro Arg 580 585 590 Leu Pro Ser Gln Leu Tyr Tyr Val Ser 595 600 <210> 298 <211> 525 <212> PRT <213> Sanguinaria canadensis <400> 298 Met Asp Ser Thr Leu Val Phe Ile Gly Leu Phe Ala Leu Leu Leu Val 1 5 10 15 Tyr Pro Leu Leu Leu Arg Arg Ser Val Leu Lys Ser Thr Ser Asn Thr 20 25 30 Asn Lys Thr Thr Tyr Lys Ser Lys Asn Gln Ala Pro Glu Ala Ala Gly 35 40 45 Ala Trp Pro Ile Ile Gly Asn Leu His Gln Leu Ala Gly Gly Gly Asn 50 55 60 Cys Leu His Lys Thr Leu Gly Ala Met Ala Asp Lys His Gly Pro Ala 65 70 75 80 Phe Thr Ile Arg Met Gly Leu His Lys Ala Leu Val Val Ser Ser Trp 85 90 95 Glu Leu Ala Lys Glu Cys Phe Thr Thr Asn Asp Val Val Phe Met Ser 100 105 110 Arg Pro His Gln Val Ala Ile Lys His Met Gly Tyr Ser Thr Ala Met 115 120 125 Phe Gly Ile Ala Pro Tyr Gly Pro Tyr Tyr Arg Glu Met Arg Lys Ile 130 135 140 Val Thr Gln Glu Leu Leu Ser Asn Arg Arg Leu Glu Leu Leu Lys His 145 150 155 160 Val Trp Ala Ser Glu Ile Asn Asn Ser Ile Lys Gln Leu Tyr Glu Lys 165 170 175 Val Glu Gly Gly Pro Val Leu Ile Glu Met Lys Arg Trp Phe Ala Asp 180 185 190 Leu Thr Leu Arg Thr Thr Val Lys Val Ile Cys Gly Gln Gln Gln Phe 195 200 205 Gly Gly Asp Asp Asp Asp Asp Glu Ala Gly Arg Phe Gln Thr Ala Leu 210 215 220 Arg Asp Phe Phe Arg Leu Leu Gly His Phe Arg Val Ala Asp Val Ile 225 230 235 240 Pro Val Leu Glu Trp Leu Asp Phe Gln Gly Tyr Lys Lys Glu Met Glu 245 250 255 Asn Asn Gly Arg Val Leu Asp Asn Leu Met Asn Lys Trp Leu Glu Glu 260 265 270 His Lys Arg Lys Arg Lys Asn Gly Gly Thr Glu Glu Asp Phe Met Asn 275 280 285 Val Met Ile Ser Lys Leu Leu Asp Asp Thr Lys Met Leu Ser Tyr Tyr 290 295 300 Asp Ala Asp Thr Ile Asn Lys Ser Thr Cys Leu Thr Leu Ile Leu Gly 305 310 315 320 Gly Ser Asp Thr Thr Met Val Ser Leu Thr Trp Ala Leu Ser Leu Leu 325 330 335 Val Asn His Pro His Val Leu Lys Lys Ala Gln Glu Glu Leu Asp Val 340 345 350 His Val Gly Lys Glu Arg Gln Val Asp Asp Ser Asp Leu Trp Ser Leu 355 360 365 Thr Tyr Leu Gln Ala Ile Ile Lys Glu Thr Leu Arg Leu Tyr Pro Pro 370 375 380 Gly Pro Leu Ser Ala Pro Arg Glu Ser Thr Asp Asn Cys Thr Ile Ala 385 390 395 400 Gly Tyr His Val Pro Ala Gly Thr Arg Leu Ile Val Asn Thr Trp Lys 405 410 415 Ile Gln Arg Asp Pro Arg Val Trp Pro Asp Pro Leu Glu Phe Lys Pro 420 425 430 Glu Arg Phe Leu Thr Thr His Val Asp Val Asp Val Lys Gly Gln Asn 435 440 445 Phe Glu Leu Leu Pro Phe Gly Ser Gly Arg Arg Ala Cys Pro Gly Ala 450 455 460 Ser Phe Ala Leu Arg Val Leu Arg Leu Ser Leu Ala Arg Leu Ile His 465 470 475 480 Gly Phe Asp Phe Lys Thr Pro Leu Asp Asp Ser Pro Ile Asp Met Val 485 490 495 Glu Ser Gly Gly Ile Thr Asn Val Lys Asp Thr Pro Leu Glu Val Leu 500 505 510 Val Thr Pro Arg Leu Pro Ser Ser Leu Tyr Val Cys Lys 515 520 525 <210> 299 <211> 532 <212> PRT <213> Sanguinaria canadensis <400> 299 Met Asp Phe Asp Leu Tyr Gln Phe Ser Lys Pro Thr Ile Phe Ile Gly 1 5 10 15 Gly Leu Phe Ala Ile Leu Leu Tyr Phe Val Leu Lys Lys Ser Ser Thr 20 25 30 Pro Lys Ser Lys Ser Lys Leu Glu Gln Pro Pro Glu Pro Ala Gly Ala 35 40 45 Trp Pro Ile Ile Gly His Leu Pro Leu Leu Gly Gly Pro Asp Leu Pro 50 55 60 His Ile Thr Leu Gly Lys Leu Ala Asp Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Ile Arg Ile Gly Val His Lys Ala Val Val Ile Asn Ser Trp Glu Val 85 90 95 Ala Lys Glu Cys Phe Thr Thr Asn Asp Lys Ala Phe Ser Ser Arg Pro 100 105 110 Arg Gln Val Ala Met Lys His Met Gly Tyr Asp Tyr Ala Met Phe Gly 115 120 125 Phe Ala Pro Tyr Gly Asn Tyr Trp Arg Glu Leu Arg Lys Ile Val Asn 130 135 140 Arg Glu Val Leu Ser His Ser Arg Ile Glu Ser Leu Tyr His Val Trp 145 150 155 160 Gly Thr Glu Ile Asn Thr Ser Val Lys Glu Leu Tyr Glu Leu Trp Gly 165 170 175 Lys Lys Ser Thr Gly Ser Gly Gly Ala Pro Val Leu Val Glu Met Lys 180 185 190 Arg Trp Phe Ser Asp Met Thr Leu Asn Met Ser Val Met Met Val Ala 195 200 205 Gly Lys Arg Tyr Asn Phe Ser Gly Asn Lys Ala Asp Asp Glu Ala Gly 210 215 220 Arg Cys Gln Asp Gly Leu Arg Asn Phe Phe Arg Leu Val Gly Leu Phe 225 230 235 240 Val Pro Ser Asp Ala Leu Pro Phe Leu Ala Trp Leu Asp Ile Gly Gly 245 250 255 Tyr Glu Lys Glu Met Lys Lys Val Ala Lys Glu Leu Asp Val Leu Val 260 265 270 Gln Glu Trp Leu Glu Glu His Lys Glu Lys Arg Leu Ala Leu Thr Ala 275 280 285 Ala Gly Lys Lys Gly Ser Glu Asn Asp Phe Met Asp Val Met Met Asn 290 295 300 Ile Leu Glu Asp Gln Lys Leu Ser Glu Phe Asp Ala Asp Thr Ile Asn 305 310 315 320 Lys Ala Thr Cys Leu Thr Leu Ile Leu Gly Gly Thr Asp Thr Asn Met 325 330 335 Val Asn Leu Val Trp Ala Leu Thr Leu Leu Val Asn Asn Gln Asp Ala 340 345 350 Leu Lys Lys Ala His Asp Glu Leu Asp Phe His Val Gly Lys Asp Arg 355 360 365 Gln Val Glu Glu Ser Asp Val Lys Asn Leu Val Tyr Ile Gln Ala Ile 370 375 380 Met Lys Glu Thr Leu Arg Leu Tyr Ser Gly Pro Leu Ser Gly Leu Arg 385 390 395 400 Glu Ser Thr Glu Asp Cys Thr Val Ala Gly Tyr Tyr Val Pro Ala Gly 405 410 415 Thr Arg Leu Ile Ile Asn Ala Ala Lys Ile His Arg Asp Pro Arg Val 420 425 430 Trp Ser Asp Pro Thr Ala Phe Lys Pro Asp Arg Phe Leu Thr Glu Gln 435 440 445 Lys Glu Met Asp Val Arg Gly Gln Asp Phe Glu Ile Leu Pro Phe Gly 450 455 460 Ala Gly Arg Arg Ile Cys Pro Gly Val Ser Phe Ala Leu Gln Val Leu 465 470 475 480 Pro Leu Ala Leu Ala Arg Leu Ile His Gly Phe Asp Phe Lys Thr Pro 485 490 495 Thr Asp Ala Pro Ile Asp Met Thr Glu Ser Pro Gly Leu Thr Asn Ala 500 505 510 Lys Ser Thr Pro Leu Glu Val Leu Val Ser Pro Arg Leu Thr Ser Lys 515 520 525 Leu Tyr Gly Cys 530 <210> 300 <211> 555 <212> PRT <213> Sanguinaria canadensis <400> 300 Met Asp Ser Ile Leu Asn Gln Phe Ile Ser Lys Pro Thr Met Val Ile 1 5 10 15 Cys Ser Leu Phe Ala Leu Leu Ser Leu Tyr Phe Leu Leu Ile Lys Arg 20 25 30 Ser Thr Thr Lys Ser Lys Leu Gln Leu Pro Pro Glu Pro Ala Gly Ala 35 40 45 Trp Pro Val Ile Gly His Leu His Gln Leu Gly Gly Pro Asn Leu Pro 50 55 60 His Ile Thr Leu Ala Ala Met Ala Asp Lys Tyr Gly Pro Ile Phe Thr 65 70 75 80 Met Lys Ile Gly Thr Tyr Arg Ala Leu Val Val Ser Ser Pro Glu Val 85 90 95 Ala Lys Glu Ile Phe Thr Thr His Asp Arg Ile Trp Ala Thr Arg Pro 100 105 110 Asn Gln Ala Ala Met Lys His Leu Gly Tyr Asp Ser Ala Met Phe Gly 115 120 125 Phe Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Lys Leu Val Asn 130 135 140 Arg Glu Leu Leu Ser Asn Thr Arg Leu Asp Leu Leu His His Val Trp 145 150 155 160 Asp Ser Glu Ile Asn Thr Ser Ile Lys Glu Leu Tyr Asp Ser Leu Ala 165 170 175 Thr Lys Asn Asn Ala Lys Gly Arg Gly Thr Gly Gly Ala Gly Ser Thr 180 185 190 Gly Pro Val Leu Val Glu Met Lys Arg Trp Phe Ala Asp Leu Thr Leu 195 200 205 Asn Ile Thr Val Arg Met Val Ala Gly Lys Arg Tyr Phe Gly Ala Asn 210 215 220 Lys Thr Ser Ser Thr Ser Thr Asp Gln Cys Asp Asn Glu Lys Gly Asp 225 230 235 240 Ser Lys Ala Trp Lys Cys Gln Arg Glu Leu Arg Asn Phe Phe Arg Leu 245 250 255 Val Gly Leu Phe Val Val Ser Asp Ala Leu Pro Phe Leu Gly Trp Leu 260 265 270 Asp Leu Gly Gly His Glu Arg Glu Met Lys Asn Thr Ala Arg Glu Leu 275 280 285 Asp Val Leu Val Gln Gly Trp Leu Asp Glu His Lys Arg Lys Arg Ser 290 295 300 Leu Ser Val Ala Glu Gly Gly Glu Lys Ile Asn Gly Glu Gln Asp Phe 305 310 315 320 Met Asp Met Met Leu Ser Thr Ile Asp Asp Ala Lys Leu Ser Gly Tyr 325 330 335 Phe Asp Ala Asp Thr Ile Asn Lys Ser Thr Cys Leu Asn Leu Ile Leu 340 345 350 Gly Gly Ser Asp Thr Met Met Val Asn Leu Ile Trp Pro Leu Thr Leu 355 360 365 Leu Val Asn Tyr Pro Asp Glu Leu Lys Lys Val Tyr Asp Glu Leu Asp 370 375 380 Thr His Val Gly Arg Asp Arg Gln Val Asp Glu Ser Asp Ile Lys Asn 385 390 395 400 Leu Val His Leu Lys Ala Val Ile Lys Glu Thr Met Arg Leu Tyr Thr 405 410 415 Gly Arg Ile Thr Gly Leu Arg Gln Ser Ser Glu Asp Cys Thr Val Ala 420 425 430 Gly Tyr His Val Pro Ala Gly Thr Arg Leu Ile Val Asn Thr Trp Lys 435 440 445 Ile Ser Arg Asp Pro Arg Tyr Trp Ser Asp Pro Leu Glu Phe Lys Pro 450 455 460 Glu Arg Phe Leu Thr Thr His Ala Asp Ile Glu Leu Thr Gly Gln Asn 465 470 475 480 Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Ala 485 490 495 Ser Phe Ala Ile Gln Leu Leu His Leu Ala Leu Ala Arg Leu Val His 500 505 510 Gly Phe Trp Phe Glu Arg Pro Ser Asp Ala Pro Ile Asp Thr Ser Glu 515 520 525 Cys Pro Gly Leu Thr Asn Phe Lys Ala Thr Pro Leu Glu Val Leu Ala 530 535 540 Thr Pro Arg Leu Pro Ser Lys Leu Tyr Val Gly 545 550 555 <210> 301 <211> 538 <212> PRT <213> Sanguinaria canadensis <400> 301 Met Asp Phe Ile Leu Asn Gln Phe Ile Ser Lys Pro Thr Met Val Leu 1 5 10 15 Gly Thr Leu Phe Ala Leu Leu Ser Leu Tyr Phe Leu Leu Ile Lys Arg 20 25 30 Ser Thr Thr Lys Ser Lys Leu Gln Leu Pro Pro Glu Pro Ala Gly Ala 35 40 45 Leu Pro Ile Ile Gly His Leu His Leu Leu Gly Gly Pro Asn Pro Pro 50 55 60 His Ile Thr Leu Ala Asn Met Ala Glu Lys Tyr Gly Pro Ile Phe Ser 65 70 75 80 Ile Lys Met Gly Leu Lys Arg Ala Leu Ile Val Ser Ser Ser Glu Val 85 90 95 Thr Lys Glu Cys Phe Thr Thr His Asp Leu Asn Phe Val Ser Arg Pro 100 105 110 Arg His Ala Ala Met Lys Leu Met Gly Tyr Asn Phe Ala Thr Phe Gly 115 120 125 Phe Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Met Ile Ile Asn 130 135 140 Arg Glu Leu Leu Ser Gln Thr Arg Ile Leu Ser Leu Glu His Val Trp 145 150 155 160 Gly Ser Glu Ile Asn Glu Ser Ile Lys Glu Leu Tyr Asp Leu Leu Thr 165 170 175 Arg Lys Gly Gly Gly Gly Gly Gly Pro Val Val Val Glu Met Lys Arg 180 185 190 Trp Phe Ala Asp Leu Thr Leu Asn Ile Ala Val Lys Met Val Cys Gly 195 200 205 Lys Arg Tyr Phe Gly Val Asn Ser Pro Ser Thr Ser Thr Thr Arg Asp 210 215 220 Val Gln Glu Asp Asp Glu Ala Arg Arg Cys Gln Lys Gly Met Arg Asn 225 230 235 240 Phe Phe Arg Leu Leu Gly Gln Phe Val Val Ser Asp Ser Ile Pro Phe 245 250 255 Leu Gly Trp Leu Asp Leu Gly Gly Tyr Gln Arg Glu Met Lys Asn Thr 260 265 270 Ala Arg Glu Leu Asp Asp Leu Met Gln Gly Trp Leu Glu Glu His Lys 275 280 285 Lys Lys Arg Leu Glu Arg Lys Thr Ala Ala Gly Glu Gln Asp Phe Met 290 295 300 Asp Val Met Leu Asn Ile Leu Glu Asp Gly Lys Leu Ser Gln Tyr Phe 305 310 315 320 Asp Thr Asp Thr Ile Asn Lys Ser Thr Cys Leu Ser Leu Ile Leu Gly 325 330 335 Gly Ser Asp Thr Thr Met Val Asn Leu Val Trp Ala Phe Ser Leu Leu 340 345 350 Val Lys His Gln Asp Ala Leu Lys Lys Thr Arg Asp Glu Leu Asp Ile 355 360 365 His Val Gly Arg Glu Arg Gln Val Glu Glu Ser Asp Ile Lys Asn Leu 370 375 380 Val Phe Leu Gln Ala Val Val Lys Glu Thr Phe Arg Leu Tyr Ala Gly 385 390 395 400 Pro Ile Ser Gly Val Arg Glu Ala Ala Glu Asp Cys Thr Ile Ala Gly 405 410 415 Tyr His Val Pro Ala Gly Thr Arg Leu Leu Ile Asn Ser Trp Lys Ile 420 425 430 His Arg Asp Pro Arg Val Trp Ser Asp Pro Leu Glu Phe His Pro Glu 435 440 445 Arg Tyr Leu Glu Ala Arg His Ala Asp Ile Asp Val Lys Gly Gln Asn 450 455 460 Phe Glu Leu Ala Pro Phe Gly Met Gly Arg Arg Val Cys Pro Gly Ser 465 470 475 480 Ala Phe Gly Leu Gln Val Leu His Leu Thr Leu Ala Arg Leu Ile His 485 490 495 Gly Phe Glu Phe Lys Thr Pro Ser Asp Ala Pro Val Asp Met Thr Glu 500 505 510 Ser Val Gly Met Ser Ile Ile Lys Ala Thr Pro Leu Glu Val Leu Val 515 520 525 Thr Pro Arg Leu Pro Ser Glu Leu Tyr Val 530 535 <210> 302 <211> 533 <212> PRT <213> Sanguinaria canadensis <400> 302 Met Asp Ile Ser Asn Leu Tyr Glu Leu Tyr Tyr Phe Ser Thr Pro Thr 1 5 10 15 Leu Phe Val Gly Gly Leu Phe Val Leu Leu Leu Ile Tyr Ser Leu Phe 20 25 30 Ser Ser Arg Pro Gly Ile Lys Ser Lys Leu Gln Gln Pro Pro Glu Pro 35 40 45 Ala Gly Ala Trp Pro Leu Ile Gly His Leu Pro Val Leu Ser Gly Pro 50 55 60 Glu Leu Pro His Val Ala Leu Gly Lys Leu Ala Asp Lys Tyr Gly Pro 65 70 75 80 Val Phe Thr Ile Arg Met Gly Val His Lys Ser Leu Val Val Ser Asp 85 90 95 Trp Glu Val Ile Lys Glu Cys Phe Thr Thr His Asp Lys Val Phe Ser 100 105 110 Ser Arg Pro Cys Gln Val Ala Met Lys Leu Met Gly Thr Ala Met Phe 115 120 125 Gly Phe Ser Pro Tyr Gly Asn Tyr Trp Arg Glu Leu Arg Lys Ile Met 130 135 140 Asn Arg Glu Val Leu Ser His Gly Arg Ile Glu Ser Leu Tyr His Val 145 150 155 160 Trp Gly Ser Glu Ile Asn Thr Ser Val Lys Glu Leu Tyr Asp Leu Trp 165 170 175 Ala Lys Lys Asn Asn Gly Gly Gly Pro Ile Leu Val Glu Met Lys Arg 180 185 190 Trp Phe Ser Asp Leu Thr Leu Asn Met Ser Val Met Met Ala Ala Gly 195 200 205 Lys Arg Tyr Asn Phe Gly Asp Asp Ser Ser Thr Thr Asn Asp Glu Ala 210 215 220 Ala Arg Cys Gln His Ala Leu Arg Glu Phe Phe Arg Leu Val Gly Leu 225 230 235 240 Phe Val Pro Ser Asp Ala Leu Pro Tyr Leu Gly Trp Leu Asp Ile Gly 245 250 255 Gly Tyr Gln Lys Gln Met Lys Lys Val Ala Arg Glu Leu Asp Asp Leu 260 265 270 Met Gln Val Trp Leu Asp Glu His Lys Lys Lys Arg Leu Ala Ala Lys 275 280 285 Ala Glu Gly Lys Lys Gly Gly Glu Gln Asp Phe Met Asp Val Met Met 290 295 300 Thr Ile Leu Glu Asp Gly Lys Leu Ser Asp Tyr Tyr Asp Ala Asp Thr 305 310 315 320 Ile Asn Lys Ala Thr Cys Leu Thr Leu Ile Leu Gly Gly Thr Asp Thr 325 330 335 Asn Met Leu Ser Leu Val Trp Thr Leu Ala Leu Leu Met Asn Asn Arg 340 345 350 Gln Ala Leu Lys Lys Val His Glu Glu Leu Asp Ile His Val Gly Arg 355 360 365 Glu Arg Gln Val Glu Glu Ser Asp Ile Lys Asn Leu Val Tyr Leu Gln 370 375 380 Ala Val Ile Lys Glu Glu Met Arg Leu Tyr Ser Gly Pro Leu Ser Gly 385 390 395 400 Leu Arg Glu Thr Thr Glu Asp Cys Thr Ile Ala Gly Tyr His Ile Pro 405 410 415 Ala Gly Thr Arg Leu Ile Ile Asn Ala Ser Lys Leu His Arg Asp Pro 420 425 430 Lys Val Trp Ser Asp Pro Leu Glu Phe Lys Pro Glu Arg Phe Leu Thr 435 440 445 Glu Asn Val Gly Val Asp Val Arg Gly Gln Asn Leu Glu Leu Gln Pro 450 455 460 Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser Phe Ala Leu Gln 465 470 475 480 Val Leu Pro Leu Thr Leu Ala Arg Leu Ile His Gly Phe Glu Leu Asn 485 490 495 Thr Pro Gly Asp Ala Pro Ile Asp Met Thr Glu Ser Pro Gly Leu Gln 500 505 510 Asn Ala Lys Leu Thr Pro Leu Glu Val Leu Ile Thr Pro Arg Leu Pro 515 520 525 Ser Lys Leu Tyr Val 530 <210> 303 <211> 535 <212> PRT <213> Sanguinaria canadensis <400> 303 Met Ile Val Ile Gly Ala Phe Leu Ala Leu Val Val Leu Val Val Tyr 1 5 10 15 Tyr Asn Leu Leu Leu Arg Arg Pro Ala Leu Leu Lys Phe Lys Asn Met 20 25 30 Lys Gln Gln Ala Pro Glu Ala Ala Ser Ala Trp Pro Ile Ile Gly His 35 40 45 Leu His Asn Leu Ala Gly Gly Ile Gly Gly Asn Gly Leu Leu His Glu 50 55 60 Lys Leu Gly Ala Met Ala Asp Lys His Gly Pro Ala Phe Ile Ile Arg 65 70 75 80 Leu Gly Leu His Lys Ala Leu Val Val Ser Ser Trp Glu Val Val Lys 85 90 95 Glu Cys Phe Thr Thr Asn Asp Val Ala Leu Ile Ser Arg Pro His Gln 100 105 110 Val Ala Ser Lys His Met Gly Tyr Gly Met Ala Met Phe Ala Phe Ala 115 120 125 Ser Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Lys Ile Val Lys Gln 130 135 140 Glu Leu Leu Ser Asn Ser Arg Leu Glu Leu Leu Lys His Val Trp Ala 145 150 155 160 Ser Glu Ile Asn Thr Ser Ile Lys Gln Leu Tyr Glu Lys Val Lys Glu 165 170 175 Gly Gly Gly Leu Pro Val Leu Val Glu Met Lys Gly Trp Phe Ala Asp 180 185 190 Leu Thr Leu Lys Met Thr Val Lys Val Ile Cys Gly Lys Arg His Phe 195 200 205 Arg Val Ala Gly Asn Val Gly Asp His Val Asp Ala Asp Asp Asp Asp 210 215 220 Glu Val Ile Gly Gly Arg Phe Glu Lys Ala Leu Arg Asp Phe Phe Arg 225 230 235 240 Leu Leu Gly Asp Ile Arg Val Val Asp Val Leu Pro Phe Leu Arg Trp 245 250 255 Leu Asp Phe Gly Val Gly Tyr Lys Lys Glu Met Glu Asn Asn Gly Arg 260 265 270 Val Leu Asp Ile Leu Met Glu Glu Trp Leu Gln Glu His Lys Arg Lys 275 280 285 Arg Met Asn Gly Gly Thr Glu Glu Asp Phe Met Asn Val Met Ile Ser 290 295 300 Lys Leu Leu Asn Asp Thr Lys Leu Leu Ser Tyr Tyr Asp Ala Asp Thr 305 310 315 320 Ile Asn Lys Ser Thr Cys Leu Thr Leu Ile Leu Gly Ala Ser Asp Thr 325 330 335 Thr Met Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Val Asn His Pro 340 345 350 Gln Val Leu Lys Arg Ala Gln Asp Glu Leu Asp Val His Val Gly Arg 355 360 365 Glu Arg Gln Val Glu Asp Ser Asp Ile Gln Asn Leu Thr Tyr Leu Gln 370 375 380 Ala Ile Ile Lys Glu Thr Leu Arg Ile Cys Pro Pro Gly Pro Ile Gln 385 390 395 400 Ala Pro His Glu Ala Lys Asp Asp Cys Thr Val Ala Gly Tyr His Val 405 410 415 Pro Ala Gly Thr Arg Leu Ile Val Asn Thr Trp Lys Ile Gln Arg Asp 420 425 430 Pro Arg Val Trp Pro Asn Pro Ser Glu Phe Lys Pro Glu Arg Phe Leu 435 440 445 Thr Thr His Val Gly Val Asp Val Arg Gly His Asn Phe Glu Leu Ile 450 455 460 Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Thr Ser Phe Ala Leu 465 470 475 480 Gln Val Val His Leu Thr Leu Ala Arg Phe Ile His Gly Phe Glu Phe 485 490 495 Lys Thr Pro Ser Asp Ala Pro Ile Asp Met Thr Glu Ser Gly Arg Ile 500 505 510 Thr Asn Val Lys Ala Thr Pro Leu Glu Val Leu Val Thr Pro Arg Ile 515 520 525 Pro Pro Ser Gly Leu Tyr Val 530 535 <210> 304 <211> 572 <212> PRT <213> Sanguinaria canadensis <400> 304 Met Asp Leu Leu Ile Phe Ser Ser Gln Leu Gln Gly Thr Val Gly Leu 1 5 10 15 Leu Ala Leu Leu Thr Phe Ser Tyr Tyr Val Trp Ile Leu Ile Ile Lys 20 25 30 Arg Ile Lys Thr Thr Asn Thr Leu Lys Val Met Asn Lys Val Ala Ala 35 40 45 Ala Ala Ala Pro Glu Val Ala Gly Ala Trp Pro Ile Val Gly His Leu 50 55 60 Pro Gln Leu Val Gly Pro Gln Gln Leu Tyr Arg Ile Leu Gly Asp Met 65 70 75 80 Ala Asp Glu Tyr Gly Pro Ile Phe Met Val Arg Phe Gly Met Tyr Pro 85 90 95 Thr Leu Val Val Ser Ser Trp Glu Met Ile Lys Glu Cys Phe Thr Thr 100 105 110 Asn Asp Arg Phe Leu Ala Ser Arg Pro Ser Ser Ala Ala Gly Lys Tyr 115 120 125 Leu Thr Tyr Asp Phe Ala Met Phe Gly Phe Ser Phe Tyr Gly Pro Tyr 130 135 140 Trp Arg Glu Ile Arg Lys Ile Ser Thr Leu Glu Leu Leu Ser His Arg 145 150 155 160 Arg Leu Glu Leu Phe Lys Asn Val Pro Phe Thr Glu Ile Asp Thr Cys 165 170 175 Ile Lys Arg Leu Tyr Gln Leu Trp Arg Val Lys Asn Asn Asp His Pro 180 185 190 Ala Ala Ala Pro Val Ile Lys Val Asp Met Ser Gln Leu Leu Arg Asp 195 200 205 Leu Thr Leu Asn Thr Ile Leu Lys Leu Val Val Gly Lys Asn Leu Phe 210 215 220 Asn Glu Lys Asp His Gly Glu Gln Glu Glu Gly Arg Lys Leu His Leu 225 230 235 240 His Lys Thr Ile Val Glu Phe Phe Lys Leu Ala Gly Val Ser Val Ala 245 250 255 Ser Asp Ala Leu Pro Phe Leu Gly Trp Leu Asp Leu Asp Gly Gln Lys 260 265 270 Lys Lys Met Lys Arg Ile Ala Lys Glu Ile Asp Leu Ile Ala Glu Arg 275 280 285 Trp Leu Gln Glu His Gln Glu Lys Lys Ser Leu Ser Asn Lys Lys Leu 290 295 300 Leu Ala Ala Gly Gly Gly Lys Val Asp Gly His Asp Asp Phe Met Asp 305 310 315 320 Val Leu Leu Phe Ile Leu Asp Asp Asp Ser Gln Phe Phe Asn Phe Ser 325 330 335 Arg Asp Thr Val Ile Lys Ala Thr Ser Trp Ala Met Ile Leu Thr Ala 340 345 350 Ala Asp Thr Thr Ser Val Ser Met Thr Trp Ala Leu Thr Leu Leu Leu 355 360 365 Thr Asn Pro Arg Val Leu Arg Lys Ala Gln Asp Glu Leu Asp Met Lys 370 375 380 Val Gly Arg Asp Arg His Val Glu Glu Arg Asp Ile Glu Asn Leu Ile 385 390 395 400 Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Pro Ala Ala 405 410 415 Pro Leu Gly Val Pro His Glu Ala Ile Gln Asp Cys Thr Val Ala Gly 420 425 430 Tyr Gln Val Arg Ala Gly Thr Arg Val Leu Val Asn Leu Trp Lys Leu 435 440 445 His Arg Asp Pro Arg Val Trp Ser Asn Pro Ser Glu Phe Arg Pro Glu 450 455 460 Arg Phe Leu Ile Asp Asp Ile Asp Glu Gln Gly Gly Gly Gly Gly Gly 465 470 475 480 Gly Gly Gly Glu Ser Thr Ala Lys Asp Phe Arg Gly Gln His Phe Glu 485 490 495 Tyr Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Ile Asn Phe 500 505 510 Ala Leu Gln Ile Val His Met Thr Leu Ala Arg Leu Leu His Ala Phe 515 520 525 Glu Leu Arg Thr Thr Thr Ser Ala Ser Leu Met Asp Met Thr Glu Glu 530 535 540 Ser Gly Leu Thr Met Pro Lys Lys Thr Pro Leu Val Val Leu Leu Gln 545 550 555 560 Pro Arg Leu Pro Leu Pro Leu Tyr Asp His His Glu 565 570 <210> 305 <211> 533 <212> PRT <213> Stylophorum diphyllum <400> 305 Met Glu Phe Leu Ser Leu Gln His Gln Leu Ile Ser Ile Phe Ala Leu 1 5 10 15 Leu Leu Ala Ser Ile Phe Leu Tyr Asn Leu Leu Lys Asn His Gly Arg 20 25 30 Lys Ser Lys Thr Ser Lys Pro Pro Ala Pro Glu Ala Ser Gly Gly Trp 35 40 45 Pro Ile Met Gly His Leu Asn Leu Phe Asn Gly Ser Glu Leu Thr His 50 55 60 Gln Ala Leu Gly Ser Met Ala Asp Lys Tyr Gly Pro Ala Phe Asn Ile 65 70 75 80 Arg Phe Gly Ser His Gln Thr Leu Val Val Ser Ser Ser Glu Ile Val 85 90 95 Lys Glu Cys Phe Thr Thr Asn Asp Arg Phe Phe Ser Asn Arg Pro Gly 100 105 110 Ser Leu Ala Ile Lys Leu Met Phe Tyr Asn Ala Asp Ser Val Gly Tyr 115 120 125 Ala Pro Tyr Gly Ala Tyr Trp Arg Asp Leu Arg Lys Ile Ser Thr Leu 130 135 140 Lys Leu Leu Ser Asn His Arg Leu Glu Thr Leu Lys His Leu Arg Thr 145 150 155 160 Ser Glu Val Glu Ser Cys Phe Lys Gln Leu Tyr Asn Gln Trp Lys Asn 165 170 175 Asn Lys Val Gly Gly Asp His Asp Phe Ala Leu Val Arg Met Asp Asn 180 185 190 Trp Phe Gly Asp Leu Thr Phe Asn Val Val Ala Arg Ile Val Ala Gly 195 200 205 Lys Lys Asn Phe Ala Gly Gly Ala Thr Asn Gly Asp Val Gly Ala Gln 210 215 220 Arg Tyr Lys Glu Ala Met Asp Glu Ala Phe Arg Leu Met Thr Ile Phe 225 230 235 240 Ala Phe Ser Asp Val Val Pro Ser Leu Gly Trp Leu Asp Lys Leu Arg 245 250 255 Gly Leu Val Gly Gly Met Lys Arg Cys Gly Ala Glu Ile Asp Ser Ile 260 265 270 Val Ala Gly Trp Val Asp Glu His Arg Leu Lys Arg Ala Ser Gly Lys 275 280 285 Gly Gly Gly His Thr Asp Leu Glu Gln Asp Phe Ile Asp Val Cys Leu 290 295 300 Glu Ile Met Glu His Ser Thr Leu Pro Gly Asp Asp Pro Glu Ile Val 305 310 315 320 Ile Lys Ser Thr Cys Leu Asp Met Ile Leu Gly Gly Ser Asp Thr Thr 325 330 335 Thr Val Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Pro His 340 345 350 Val Leu Lys Arg Ala Arg Glu Glu Leu Asp Thr His Val Gly Lys Asp 355 360 365 Arg Gln Val Asp Asp Ser Asp Met Ser Asn Leu Val Tyr Ile Gln Ala 370 375 380 Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Leu Ile Glu 385 390 395 400 Arg Arg Thr Ser Glu Asp Cys Glu Val Gly Gly Phe His Val Pro Ala 405 410 415 Gly Thr Arg Leu Leu Val Asn Leu Trp Lys Met Gln Arg Asp Gly Ser 420 425 430 Val Tyr Lys Glu Asp Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Thr 435 440 445 Ser Asn Ala Asp Val Asp Leu Lys Gly Gln Asn Tyr Glu Leu Ile Pro 450 455 460 Phe Gly Ala Gly Arg Arg Ile Cys Pro Gly Val Ser Phe Ala Val Gln 465 470 475 480 Leu Met His Leu Val Leu Ala Arg Leu Val His Gly Phe Glu Met Lys 485 490 495 Thr Ala Gly Asp Ala Lys Val Asp Met Thr Glu Ser Ala Gly Leu Ile 500 505 510 Ser His Lys Val Thr Pro Leu Glu Val Leu Leu Lys Pro Cys Leu Ala 515 520 525 Ile Gln Gln Ala Leu 530 <210> 306 <211> 533 <212> PRT <213> Stylophorum diphyllum <400> 306 Met Asp Ser Phe Leu Leu Ile Gln Trp Phe Ala Ala Ser Met Ala Ala 1 5 10 15 Leu Leu Ala Phe Val Phe Leu Tyr Asn Leu Val Trp Ser Ser Ser Arg 20 25 30 Thr Thr Lys Gly Lys Asn Ile Arg Lys Ala Pro Met Ala Ala Gly Ala 35 40 45 Trp Pro Ile Leu Gly His Leu His Leu Phe Gly Ser Gly Glu Leu Pro 50 55 60 His Lys Met Leu Ser Thr Met Ala Glu Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Met Lys Phe Gly Lys His Thr Thr Leu Val Val Ser Asp Thr Arg Ile 85 90 95 Val Lys Glu Cys Phe Thr Thr His Asp Thr Leu Phe Ala Asn Arg Pro 100 105 110 Ser Thr Thr Ala Phe Asp Leu Met Thr Tyr Ala Asn Asp Ser Val Ala 115 120 125 Phe Thr Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Lys Ile Ser Thr 130 135 140 Leu Lys Leu Leu Ser Asn His Arg Leu Gln Ser Ile Lys Glu Ile Arg 145 150 155 160 Val Ser Glu Val Asn Val Cys Phe Arg Glu Leu Tyr Glu Ser Cys Lys 165 170 175 Ser Lys Thr Asp Ala Thr Pro Val Leu Val Asp Met Lys Lys Trp Phe 180 185 190 Glu Glu Val Ser Asn Asn Ile Val Met Arg Val Ile Val Gly Arg Gln 195 200 205 Asn Phe Gly Ser Lys Ile Val Gln Gly Glu Asp Glu Ala Val Asn Tyr 210 215 220 Lys Lys Val Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Met Leu 225 230 235 240 Ser Asp Val Ala Pro Val Leu Gly Trp Leu Asp Met Phe Gln Gly Asn 245 250 255 Lys Ser Ala Met Lys Arg Asn Ala Lys Lys Val Asp Thr Ile Leu Glu 260 265 270 Gly Trp Leu Glu Glu His Arg Met Lys Lys Ser Ala Ser Gly Ile Ser 275 280 285 Ser Ala Gly Glu Asn Asp Gln Asp Phe Met Asp Val Met Leu Ser Ile 290 295 300 Ile Glu Glu Thr Lys Leu Ser Gly Arg Asp Ala Asp Thr Val Ile Lys 305 310 315 320 Ala Thr Cys Leu Ala Met Ile Met Gly Gly Thr Asp Thr Thr Ala Val 325 330 335 Ser Leu Thr Trp Ile Val Ser Leu Leu Met Asn His Arg His Val Leu 340 345 350 Lys Lys Ala Arg Glu Glu Leu Asp Ala Leu Val Gly Lys Asp Arg Gln 355 360 365 Val Glu Asp Ser Asp Leu Lys Asn Leu Val Tyr Met Asn Ala Ile Val 370 375 380 Lys Glu Thr Met Arg Met Tyr Pro Leu Gly Ala Met Leu Glu Arg Glu 385 390 395 400 Thr Lys Glu Asp Cys Glu Val Gly Gly Phe Gln Val Gln Gly Gly Thr 405 410 415 Arg Leu Leu Val Asn Val Trp Lys Leu Gln Arg Asp Pro Asn Val Trp 420 425 430 Thr Asp Pro Thr Glu Phe Lys Pro Glu Arg Phe Leu Asn Glu Asn Ala 435 440 445 Asp Ile Asp Val Gly Gly Gln His Phe Glu Leu Leu Pro Phe Gly Ala 450 455 460 Gly Arg Arg Ala Cys Pro Gly Val Ser Phe Ala Leu Gln Phe Met His 465 470 475 480 Leu Val Leu Ala Arg Leu Ile His Gly Tyr Glu Leu Gly Thr Gln Asn 485 490 495 Asp Ala Asp Val Asp Leu Thr Glu Ser Thr Glu Gly His Val Asn His 500 505 510 Lys Ala Ser Pro Leu Asp Leu Leu Leu Thr Pro Arg Leu Asn Asn Pro 515 520 525 Asn Leu Tyr Asp Tyr 530 <210> 307 <211> 568 <212> PRT <213> Stylophorum diphyllum <400> 307 Met Thr Ile Gly Ala Leu Ala Leu Leu Ser Phe Ile Tyr Phe Leu Arg 1 5 10 15 Val Ser Val Ile Lys Arg Thr Lys Tyr Thr Asn Thr Ala Val Thr Ala 20 25 30 Thr Asn Lys Leu Glu Asn Asp Glu Asp Glu Ala Asn His Ser Lys Arg 35 40 45 Val Val Ala Pro Pro Glu Val Ala Gly Ala Trp Pro Ile Leu Gly His 50 55 60 Leu Pro Gln Leu Val Gly Leu Lys Gln Pro Leu Phe Arg Val Leu Gly 65 70 75 80 Asp Met Ala Asp Lys Tyr Gly Pro Ile Phe Ile Val Arg Phe Gly Met 85 90 95 Tyr Pro Thr Leu Val Val Ser Ser Trp Glu Met Ala Lys Glu Cys Phe 100 105 110 Thr Thr Asn Asp Arg Val Leu Ala Ser Arg Pro Ala Ser Ala Ser Gly 115 120 125 Lys Tyr Leu Thr Tyr Asn Tyr Ala Met Phe Gly Phe Thr Asn Gly Pro 130 135 140 Tyr Trp Arg Glu Ile Arg Lys Ile Ser Met Leu Glu Leu Leu Ser His 145 150 155 160 Arg Arg Val Glu Leu Leu Lys His Val Pro Ser Thr Glu Ile Asp Ser 165 170 175 Ser Ile Lys Gln Leu Tyr His Leu Trp Val Glu Asn Gln Asn Gln Asn 180 185 190 Lys Gln Gly Asp His Gln Val Lys Val Asp Met Ser Gln Leu Leu Arg 195 200 205 Asp Leu Thr Leu Asn Ile Val Leu Lys Leu Val Val Gly Lys Arg Leu 210 215 220 Phe Asn Asn Asn Asp Met Asp His Glu Gln Asp Glu Ala Ala Arg Lys 225 230 235 240 Leu Gln Lys Thr Met Val Glu Leu Ile Lys Val Ala Gly Ala Ser Val 245 250 255 Ala Ser Asp Ala Leu Pro Phe Leu Gly Trp Leu Asp Val Asp Gly Leu 260 265 270 Lys Arg Thr Met Lys Arg Ile Ala Lys Glu Ile Asp Val Ile Ala Glu 275 280 285 Arg Trp Leu Gln Glu His Arg Gln Lys Lys Leu Thr Ser Asn Asp Lys 290 295 300 Gly Gly Ser Asn Asn Ile Gln Gly Gly Gly Gly Asp Asn Asp Phe Met 305 310 315 320 Asp Val Met Leu Ser Ile Leu Asp Asp Asp Ser Asn Phe Phe Ile Asn 325 330 335 Tyr Asn Arg Asp Thr Val Ile Lys Ala Thr Ser Leu Thr Met Ile Leu 340 345 350 Ala Gly Ser Asp Thr Thr Thr Leu Ser Leu Thr Trp Ala Leu Thr Leu 355 360 365 Leu Ala Thr Asn Pro Gly Ala Leu Arg Lys Ala Gln Asp Glu Leu Asp 370 375 380 Thr Lys Val Gly Arg Asp Arg Gln Val Asp Glu Arg Asp Ile Lys Asn 385 390 395 400 Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Met Tyr Pro 405 410 415 Ala Ala Pro Leu Ala Ile Pro His Glu Ala Thr Gln Asp Cys Ile Val 420 425 430 Gly Gly Tyr His Val Thr Ala Gly Thr Arg Val Trp Val Asn Leu Trp 435 440 445 Lys Leu Gln Arg Asp Pro His Ala Trp Pro Asn Pro Ser Glu Phe Arg 450 455 460 Pro Glu Arg Phe Leu Ala Val Glu Asn Asp Cys Lys Gln Gln Gly Thr 465 470 475 480 Cys Asp Gly Glu Ala Ala Asn Met Asp Phe Arg Gly Gln His Phe Glu 485 490 495 Tyr Met Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Ile Asn Phe 500 505 510 Ala Ile Gln Ile Ile His Met Thr Leu Ala Arg Leu Leu His Ser Phe 515 520 525 Glu Leu Arg Val Pro Glu Glu Glu Val Ile Asp Met Ala Glu Asp Ser 530 535 540 Gly Leu Thr Ile Ser Lys Val Thr Pro Leu Glu Leu Leu Leu Thr Pro 545 550 555 560 Arg Leu Pro Leu Pro Leu Tyr Ile 565 <210> 308 <211> 527 <212> PRT <213> Stylophorum diphyllum <400> 308 Met Ala Ala Leu Leu Pro Leu Ala Phe Leu Tyr Asn Ile Phe Leu Ala 1 5 10 15 Ser Ser Ser Lys Ala Thr Ser Lys Arg Thr Ile Ser Thr Lys Lys Pro 20 25 30 Pro Met Val Ala Gly Ala Trp Pro Ile Leu Gly His Leu His Leu Phe 35 40 45 Lys Glu Gly Glu Leu Pro His Gln Met Leu Lys Ser Met Ala Asp Lys 50 55 60 Tyr Gly Pro Ala Phe Leu Met Lys Phe Gly Gln His Gln Ser Leu Val 65 70 75 80 Val Ser Asp Tyr Arg Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Thr 85 90 95 Leu Phe Cys Asn Arg Pro Ser Thr Thr Ala Phe Asp Val Met Thr Tyr 100 105 110 Ala Asn Glu Ser Val Ala Phe Thr Glu Tyr Ser Pro Tyr Trp Arg Glu 115 120 125 Leu Arg Lys Ile Ser Thr Leu Lys Leu Leu Ser Asn Asn Arg Leu Gln 130 135 140 Ala Ile Lys Asn Leu Arg Glu Glu Glu Val Asp Val Ser Phe Lys Gly 145 150 155 160 Leu Tyr Asp Ser Trp Lys Asn Lys Asn Asn Lys Ser Thr Ala Gly Ser 165 170 175 Val Gly Asp Glu Arg Ala Pro Val Leu Val Asp Met Lys Lys Trp Phe 180 185 190 Glu Glu Val Ser Asn Asn Val Val Ile Arg Val Ile Val Gly Lys Cys 195 200 205 Asn Phe Gly Thr Lys Ile Val Gln Gly Glu Lys Glu Gly Val Glu Tyr 210 215 220 Lys Thr Ile Met Asp Glu Leu Leu Arg Leu Ala Ser Leu Ser Leu Leu 225 230 235 240 Ser Asp Phe Ala Pro Ile Leu Gly Leu Leu Asp Phe Phe Gln Gly His 245 250 255 Val Arg Thr Met Lys Arg Asn Gly Lys Lys Leu Asp Val Leu Leu Gln 260 265 270 Arg Trp Leu Glu Glu His Lys Arg Lys Lys Ser Thr Pro Glu Asp Glu 275 280 285 Gln Asp Phe Met Asp Val Met Leu Ser Ile Ile Glu Glu Ser Lys Leu 290 295 300 Ser Gly Tyr Asp Ala Asp Thr Val Ile Lys Ala Thr Cys Leu Ala Met 305 310 315 320 Ile Met Gly Gly Thr Asp Thr Ser Ala Val Ser Leu Thr Trp Ile Val 325 330 335 Ser Leu Leu Met Asn Asn Arg Gln Ala Leu Lys Lys Ala Arg Glu Glu 340 345 350 Leu Asp Ala Gln Val Gly Lys Asp Arg Gln Val Glu Asp Ser Asp Leu 355 360 365 Lys Asn Leu Val Tyr Leu Asn Ala Ile Val Lys Glu Thr Met Arg Leu 370 375 380 Tyr Pro Leu Gly Thr Leu Leu Glu Arg Glu Thr Lys Glu Asp Cys Glu 385 390 395 400 Val Gly Gly Phe His Leu Glu Gly Gly Thr Arg Leu Leu Val Asn Val 405 410 415 Trp Met Val Gln Arg Asp Pro Asp Val Trp Thr Asp Pro Thr Lys Phe 420 425 430 Ile Pro Glu Arg Phe Leu Thr Glu Lys Ala Asp Ile Asp Val Gly Gly 435 440 445 Gly Asn Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Val Cys Pro 450 455 460 Gly Val Ser Phe Ala Leu Gln Phe Leu Asn Leu Val Leu Ala Arg Leu 465 470 475 480 Ile His Gly Tyr Glu Leu Gly Thr Pro Glu Asp Ala Asp Val Asp Leu 485 490 495 Thr Glu Ser Pro Glu Gly His Val Asn His Lys Ala Ser Pro Leu Glu 500 505 510 Leu Leu Leu Thr Pro Arg Leu Ser Asn Pro Lys Leu Tyr Asp Tyr 515 520 525 <210> 309 <211> 547 <212> PRT <213> Stylophorum diphyllum <400> 309 Met Asp Ser Asp His Gln Leu Leu Ser Tyr Gln Phe Ser Ala Ser Asn 1 5 10 15 Met Phe Ile Ser Leu Phe Ala Phe Val Leu Tyr Tyr Leu Leu Val Trp 20 25 30 Arg Pro Thr Lys Ser Asn Leu Lys Met Lys Thr Ile Ser Ser Glu Asp 35 40 45 Lys Gln Ala Pro Glu Leu Ala Gly Ser Trp Pro Val Ile Gly His Leu 50 55 60 His Leu Leu His Gly Pro Asn Pro Leu His Val Ala Leu Gly Ala Met 65 70 75 80 Ala Asp Lys Tyr Gly Pro Ala Phe Thr Val Arg Val Gly Val His Arg 85 90 95 Thr Leu Val Val Ser Ser Trp Glu Val Ala Lys Glu Cys Phe Thr Thr 100 105 110 Asn Asp Lys Val Phe Val Ser Arg Pro Arg Gln Ala Ala Gly Lys His 115 120 125 Met Gly Tyr Asn Asn Ala Met Leu Arg Phe Ala Ser Asp Thr Pro Tyr 130 135 140 Trp Thr Gln Ile Arg Lys Met Leu Lys Arg Asp Leu Leu Ser Asn Asn 145 150 155 160 Arg Ile Glu Leu Leu Asn His Ala Trp His Ser Glu Ile Asn Thr Ser 165 170 175 Ile Lys Glu Leu Tyr Glu Met Asn Trp Ser Ser Gly Glu Gly Arg Arg 180 185 190 Gly Gly Ile Arg Pro Pro Val Ser Val Asp Met Lys Gln Trp Leu Gly 195 200 205 Asp Leu Thr Leu Asn Met Ser Val Lys Met Ile Ala Gly Lys Arg Cys 210 215 220 Phe Gly Ser Gly Val Ser Ala Cys Asp Glu Gly Glu Ala Arg Arg Cys 225 230 235 240 Gln Lys Gly Leu Lys Asp Phe Val Arg Leu Met Gly Gln Ile Val Val 245 250 255 Ser Asp Ala Ile Pro Phe Leu Gly Trp Leu Asp Leu Asp Gly Tyr Glu 260 265 270 Gly Glu Met Lys Arg Ala Gly Lys Glu Leu Asp Arg Leu Leu Gly Gly 275 280 285 Trp Leu Glu Glu His Lys Met Lys Arg Ser Pro Gly Asp Glu Ala Ser 290 295 300 Ala Gln Lys Asp Trp Met Asp Leu Met Leu Ser Val Leu Gly Asp Gly 305 310 315 320 Lys Leu Asp Gly Tyr Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr Cys 325 330 335 Leu Ala Leu Leu Gln Gly Gly Ser Ile Trp Thr Met Leu Thr Leu Leu 340 345 350 Trp Ala Phe Ala Leu Leu Val Asn His Pro His Val Met Lys Lys Ala 355 360 365 His Asp Glu Leu Asp Asn His Val Gly Arg Glu Arg Gln Val Glu Glu 370 375 380 Ser Asp Ile Lys Asn Leu Thr Tyr Leu Gln Ala Ile Val Lys Glu Ala 385 390 395 400 Met Arg Leu Tyr Ser Val Ser Met Arg Leu Leu Glu Ser Thr Ala Asp 405 410 415 Cys Thr Val Ser Gly Tyr Asp Val Pro Ala Gly Thr Arg Leu Ile Val 420 425 430 Asn Thr Trp Lys Ile Gln Arg Asp Pro Arg Val Trp Ser Asp Pro Ser 435 440 445 Glu Phe His Pro Glu Arg Phe Leu Thr His Arg His Arg Asp Met Asp 450 455 460 Leu Tyr Gly Gln Asn Phe Glu Ile Thr Pro Phe Gly Ser Gly Arg Arg 465 470 475 480 Ser Cys Pro Gly Ser Ser Leu Gly Leu Gln Met Val Gln Leu Thr Ile 485 490 495 Ala Arg Leu Leu His Gly Phe Glu Phe Lys Thr Pro Ser Asp Ala Pro 500 505 510 Ile Asp Met Thr Glu Ser Val Gly Val Glu Asn Met Val Lys Ala Thr 515 520 525 Pro Ile Glu Val Leu Leu Thr Pro Arg Leu Leu Asp Glu Val Tyr Ala 530 535 540 Phe Tyr Asn 545 <210> 310 <211> 561 <212> PRT <213> Stylophorum diphyllum <400> 310 Phe Cys Gln Phe Gln Gly Ile Val Gly Ile Leu Leu Ala Phe Leu Thr 1 5 10 15 Phe Leu Tyr Tyr Leu Trp Arg Ala Ser Ile Thr Gly Leu Arg Thr Lys 20 25 30 Pro Lys His Asn Asp Phe Lys Val Thr Lys Ala Ala Pro Glu Ala Asp 35 40 45 Gly Ala Trp Pro Ile Val Gly His Phe Ala Gln Phe Ile Gly Pro Arg 50 55 60 Pro Leu Phe Arg Ile Leu Gly Asp Met Ala Asp Lys Tyr Gly Ser Ile 65 70 75 80 Phe Met Val Arg Phe Gly Met Tyr Pro Thr Leu Val Val Ser Ser Trp 85 90 95 Glu Met Ala Lys Glu Cys Phe Thr Thr Asn Asp Arg Phe Leu Ala Ser 100 105 110 Arg Pro Ala Ser Ala Ala Gly Lys Tyr Leu Thr Tyr Asp Phe Ala Met 115 120 125 Leu Ser Phe Ser Phe Tyr Gly Pro Tyr Trp Arg Glu Ile Arg Lys Ile 130 135 140 Ser Met Leu Glu Leu Leu Ser His Arg Arg Val Glu Leu Leu Lys His 145 150 155 160 Val Pro Ser Thr Glu Ile Asp Ser Ser Ile Lys Gln Leu Tyr His Leu 165 170 175 Trp Val Glu Asn Gln Asn Gln Asn Lys Gln Gly Asp His Gln Val Lys 180 185 190 Val Asp Met Ser Gln Leu Leu Arg Asp Leu Thr Leu Asn Ile Val Leu 195 200 205 Lys Leu Val Val Gly Lys Arg Leu Phe Asn Asn Asn Asp Met Asp His 210 215 220 Glu Gln Asp Glu Ala Ala Arg Lys Leu Gln Lys Thr Met Val Glu Leu 225 230 235 240 Ile Lys Val Ala Gly Ala Ser Val Ala Ser Asp Ala Leu Pro Phe Leu 245 250 255 Gly Trp Leu Asp Val Asp Gly Leu Lys Arg Thr Met Lys Arg Ile Ala 260 265 270 Lys Glu Ile Asp Val Ile Ala Glu Arg Trp Leu Gln Glu His Arg Gln 275 280 285 Lys Lys Leu Thr Ser Asn Asp Lys Gly Gly Ser Asn Asn Ile Gln Gly 290 295 300 Gly Gly Gly Asp Asn Asp Phe Met Asp Val Met Leu Ser Ile Leu Asp 305 310 315 320 Asp Asp Ser Asn Phe Phe Ile Asn Tyr Asn Arg Asp Thr Val Ile Lys 325 330 335 Ala Thr Ser Leu Thr Met Ile Leu Ala Gly Ser Asp Thr Thr Thr Leu 340 345 350 Ser Leu Thr Trp Ala Leu Thr Leu Leu Ala Thr Tyr Pro Leu Cys Ala 355 360 365 Leu Arg Lys Ala Gln Asp Glu Leu Asp Thr Lys Val Gly Arg Asp Arg 370 375 380 Gln Val Asp Glu Arg Asp Ile Lys Asn Leu Val Tyr Leu Gln Ala Ile 385 390 395 400 Val Lys Glu Thr Leu Arg Met Tyr Pro Ala Ala Pro Leu Ala Ile Pro 405 410 415 His Glu Ala Thr Gln Asp Cys Ile Val Gly Gly Tyr His Val Thr Ala 420 425 430 Gly Thr Arg Val Trp Val Asn Leu Trp Lys Leu Gln Arg Asp Pro His 435 440 445 Ala Trp Pro Asn Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Ala Val 450 455 460 Glu Asn Asp Cys Lys Gln Gln Gly Thr Cys Asp Gly Glu Ala Ala Asn 465 470 475 480 Met Asp Phe Arg Gly Gln His Phe Glu Tyr Met Pro Phe Gly Ser Gly 485 490 495 Arg Arg Met Cys Pro Gly Ile Asn Phe Ala Ile Gln Ile Ile His Met 500 505 510 Thr Leu Ala Arg Leu Leu His Ser Phe Glu Leu Arg Val Pro Glu Glu 515 520 525 Glu Val Ile Asp Met Ala Glu Asp Ser Gly Leu Thr Ile Ser Lys Val 530 535 540 Thr Pro Leu Glu Leu Leu Leu Thr Pro Arg Leu Pro Leu Pro Leu Tyr 545 550 555 560 Ile <210> 311 <211> 522 <212> PRT <213> Tinosporia cardofolia <400> 311 Met Asp Leu Pro Gln Pro Thr Ile Ser Thr Thr Leu Leu Ala Phe Phe 1 5 10 15 Ser Ile Leu Leu Phe Ile Ile Cys Tyr Arg Leu Leu Ser Thr Lys Ser 20 25 30 Asp Lys Arg Ser Ser Ser Ser Arg Asn Lys Pro Leu Asp Ala Pro Gly 35 40 45 Ala Trp Pro Val Ile Gly His Leu His Leu Leu Asn Gly Val Ile Gln 50 55 60 His Glu Ile Leu Gly Thr Met Ala Asp Lys Tyr Gly Pro Ala Phe Thr 65 70 75 80 Ile Trp Leu Gly Thr Arg Pro Ala Leu Val Val Ser Asn Trp Glu Val 85 90 95 Ala Lys Glu Cys Phe Thr Thr Cys Asp Lys Ala Leu Ala Ser Arg Pro 100 105 110 Pro Ser Leu Ala Leu Lys Ile Met Gly Tyr Asn Asp Ala Leu Phe Ala 115 120 125 Phe Ala Pro Tyr Gly Gln Tyr Trp Arg Glu Leu Arg Lys Ile Val Met 130 135 140 Ile Glu Leu Leu Ser Ser Arg Gln Leu Asp Ser Leu Lys His Val Trp 145 150 155 160 Asp Ser Glu Ile Asp Ser Cys Ile Glu Asp Leu Tyr Glu Lys Cys Lys 165 170 175 Gly His Arg Glu Thr Val Leu Val Asn Met Lys Gln Trp Phe Ala Asp 180 185 190 Leu Met Met Asn Val Val Val Arg Met Val Val Gly Lys Leu Asn Phe 195 200 205 Gly Lys Thr Lys Glu Gly Asp Asp Glu Val Ala Lys Ala His Gln Gly 210 215 220 Gln Leu Lys Ala Met Arg Glu Phe Phe Lys Trp Val Asp Val Phe Met 225 230 235 240 Ile Glu Asp Ala Phe Pro Ile Leu Thr Trp Leu Asp Pro Gln Gly Tyr 245 250 255 Gln Arg Lys Met Lys Asn Ile Ala Lys Asp Leu Asp Ser Leu Val Gln 260 265 270 Gly Trp Leu Asp Glu His Lys Leu Lys Gln Lys Thr Gly Asp Asn Gly 275 280 285 Lys Lys Asp Phe Met Asp Ile Met Leu Ser Ala Val Lys Asp Ala Ser 290 295 300 Ile Ser Asp Arg Asp Asp Glu Thr Ile Cys Lys Ala Thr Cys Leu Asn 305 310 315 320 Ile Ile Leu Gly Ala Ser Asp Thr Thr Thr Val Thr Leu Thr Trp Thr 325 330 335 Leu Ser Leu Leu Leu Asn Asn Arg His Val Leu Arg Lys Ala Gln Glu 340 345 350 Glu Leu Gln Ala Gln Val Gly Lys His Arg Cys Val Glu Glu Ser Asp 355 360 365 Val Lys Asn Leu Val Tyr Leu His Ala Ile Ile Lys Glu Val Leu Arg 370 375 380 Leu Tyr Pro Ala Val Pro Leu Leu Gly Pro Arg Glu Ser Val Ala Asp 385 390 395 400 Thr Val Val Ala Gly Tyr His Ile Pro Ala Gly Thr Arg Leu Ile Val 405 410 415 Asn Leu Trp Lys Ile His Arg Asp Pro Leu Asn Trp Pro Asp Pro Leu 420 425 430 Glu Phe Arg Pro Glu Arg Phe Leu Thr Thr His Lys Asp Thr Asp Val 435 440 445 Trp Gly Gln Asn Phe Glu Leu Thr Pro Phe Gly Ser Gly Arg Arg Ile 450 455 460 Cys Pro Gly Ile Ser Phe Ala Leu Gln Val Ile Pro Leu Thr Leu Ala 465 470 475 480 Arg Leu Leu His Gly Phe Glu Leu Thr Thr Pro Gly Ser Ala Pro Val 485 490 495 Asp Met Thr Glu Ser Thr Gly Leu Thr Asn Leu Lys Ala Thr Pro Leu 500 505 510 Asp Val Phe Ile Ser Pro Arg Leu Val Leu 515 520 <210> 312 <211> 526 <212> PRT <213> Tinosporia cardofolia <400> 312 Met Glu Ser Ile Pro Thr Ala Ile Asn Val Leu Leu Ala Phe Val Ser 1 5 10 15 Ile Tyr Tyr Leu Leu Lys Phe Arg Lys Lys Ile Ile Thr Thr Thr Arg 20 25 30 Asp Ile Lys Lys Ile Lys Ala Pro Glu Leu Lys Gly Ala Trp Pro Ile 35 40 45 Ile Gly His Leu His Leu Phe Arg Ser Asp Asp Leu Leu His Arg Lys 50 55 60 Leu Gly Ala Met Ala Asp Glu Leu Gly Pro Ala Phe Met Ile Arg Leu 65 70 75 80 Gly Met Arg Pro Ala Leu Val Val Ser Asn Trp Gln Thr Ala Lys Glu 85 90 95 Cys Phe Thr Thr Lys Asp Arg Ile Phe Ala Thr Arg Pro Asp Ser Val 100 105 110 Ala Ala Lys His Met Gly Tyr Glu Asn Leu Ile Leu Gly Phe Thr Arg 115 120 125 Tyr Gly Pro Tyr Trp Arg Glu Val Arg Lys Ile Val Thr Leu Glu Leu 130 135 140 Leu Ser Ser His Arg Leu His Leu Leu Arg His Val Arg Ile Ser Glu 145 150 155 160 Ile Asp Met Ser Ile Lys Lys Leu His Gln Leu Cys Thr Ser Asn Asp 165 170 175 Asn Lys Tyr Ser Ser Val Val Val Asp Leu Asn Gln Trp Phe Lys Ala 180 185 190 Leu Thr Leu Asn Ile Ile Val Arg Ile Ile Ala Gly Lys Arg Tyr Tyr 195 200 205 Gly Gly Asp Leu Glu Asp Asp Glu Glu Ser Arg Arg Trp Lys Lys Ala 210 215 220 Leu Asn Glu Phe Met His Leu Phe Gly Ile Phe Ser Val Ser Asp Ala 225 230 235 240 Ile Pro Gln Leu Gln Gly Met Asp Val Gln Gly His Glu Arg Val Met 245 250 255 Lys Arg Thr Gly Lys Asp Ile Asp Val Ile Leu Ser Lys Trp Leu Glu 260 265 270 Glu His Arg Arg Lys Lys Ile Arg Val Asn Arg Glu Gly Ala Glu Lys 275 280 285 Asp Phe Ile Asp Ile Met Leu Asp Leu Ile Lys Glu Asn Val Asn Ile 290 295 300 Ser Gly His Asp Ala Asp Asn Val Val Lys Ala Thr Cys Leu Ala Leu 305 310 315 320 Val Ser Ala Gly Ser Asp Thr Thr Met Ile Thr Leu Thr Trp Ala Val 325 330 335 Ser Leu Leu Leu Asn Asn His Arg Val Leu Glu Lys Ala Gln Ala Glu 340 345 350 Leu Asp Asn Gln Ile Gly Ile Asn Arg Val His Val Glu Glu Glu Asp 355 360 365 Ile Lys Asn Leu Pro Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg 370 375 380 Leu Tyr Pro Ala Ala Pro Phe Ser Leu Pro His Glu Ala Met Glu Glu 385 390 395 400 Cys Thr Val Ala Gly Phe His Val Pro Lys Gly Thr Arg Leu Ile Thr 405 410 415 Asn Ile Trp Lys Leu Gln Arg Asp Pro Gln Val Trp Glu Asp Pro Leu 420 425 430 Glu Phe Arg Pro Glu Arg Phe Leu Thr Ser Asp Arg Arg His Val Asp 435 440 445 Phe Arg Gly Gln His Phe Glu Leu Ile Pro Phe Gly Ser Gly Arg Arg 450 455 460 Met Cys Pro Gly Ile Ser Phe Ala Ile Gly Val Leu His Leu Thr Leu 465 470 475 480 Ala Arg Leu Leu His Glu Phe His Leu Gly Thr Pro Thr Asn Ala Pro 485 490 495 Ile Asp Met Asn Glu Asn Pro Gly Ile Thr Leu Glu Lys Ala Asp Pro 500 505 510 Leu His Pro Leu Val Ser Pro Arg Leu Thr Ser Ile Asn Tyr 515 520 525 <210> 313 <211> 538 <212> PRT <213> Tinosporia cardofolia <400> 313 Met Asp Ser Gln Leu Thr Ser Phe Gln Ser Lys Pro Ser Ala Tyr Leu 1 5 10 15 Ile Ala Ala Leu Leu Ala Leu Val Ser Ala Tyr Tyr Leu Ile Ile Lys 20 25 30 Lys Lys Ser Arg Ala Thr Tyr Gln Asp Asn Tyr Gly Lys Ile Arg Ala 35 40 45 Pro Glu Pro Lys Gly Ala Trp Pro Ile Ile Gly His Leu Leu Leu Phe 50 55 60 Lys Pro Asn Asp Val Leu Tyr Gln Lys Leu Ser Ser Leu Ala Asp Glu 65 70 75 80 Leu Gly Pro Ala Phe Met Ile Arg Leu Gly Met Arg Ser Ala Leu Val 85 90 95 Ile Ser Asn Trp Glu Ile Ala Lys Glu Cys Phe Thr Thr Asn Asp Arg 100 105 110 Ile Phe Ala Thr Arg Pro Lys Leu Met Ala Ser Lys His Met Gly Tyr 115 120 125 Gly Gly Ala Glu Val Gly Val Ala Pro Tyr Gly Pro Tyr Trp Arg Glu 130 135 140 Val Arg Lys Ile Ile Thr Val Glu Leu Val Ser Asn His Arg Leu Asn 145 150 155 160 Leu Leu Lys Asp Val Arg Ile Ser Glu Ile Asp Met Ser Leu Lys Glu 165 170 175 Leu Tyr Asp Leu Cys Met Lys Lys Lys Lys Asn Gly Ser Gly Glu Glu 180 185 190 Ser Ser Arg Val Glu Leu Asp Leu Tyr Gln Trp Phe Lys Asp Met Ser 195 200 205 Leu Asn Ile Leu Val Lys Leu Ile Ala Gly Lys Arg Tyr Tyr Gly Val 210 215 220 Ala Ala Ala Ala Asn Glu Asp Glu Glu Ser Arg Arg Trp Lys Lys Ala 225 230 235 240 Thr Glu Glu Ser Val Phe Leu Phe Gly Gln Phe Val Val Gly Asp Ala 245 250 255 Ile Pro Leu Leu Glu Gly Val Asp Val Gln Gly Leu Glu Arg Ala Met 260 265 270 Lys Lys Thr Gly Lys Glu Ile Asp Ala Val Leu Ser Lys Trp Val Glu 275 280 285 Glu His Arg Met Lys Lys Gln Gln Leu Asn Gly Ala Arg Asp Glu Gln 290 295 300 Asp Phe Ile Asp Val Met Leu Asn Ile Ile Lys Asp Gly Lys Ile Ser 305 310 315 320 Asp His Asp Ala Asp Thr Ile Val Lys Ala Thr Cys Met Ser Leu Val 325 330 335 Gln Ala Gly Ser Asp Thr Thr Met Leu Thr Leu Thr Trp Ala Val Cys 340 345 350 Leu Leu Leu Asn Asn Cys Asn Val Leu Glu Lys Ala Gln Ala Glu Leu 355 360 365 Asp Asp Gln Ile Gly Arg Ser Lys Gly Arg Ile Val Glu Glu Asp Asp 370 375 380 Ile Lys Lys Leu Pro Tyr Leu Gln Ala Ile Val Lys Glu Thr Trp Arg 385 390 395 400 Leu Tyr Pro Pro Ala Pro Leu Ala Val Pro Arg Glu Ala Met Glu Asp 405 410 415 Cys Thr Val Ala Gly Phe His Val Pro Lys Gly Thr Arg Leu Met Thr 420 425 430 Asn Ile Trp Lys Leu His Arg Asp Pro Lys Val Trp Asp Asp Ser Leu 435 440 445 Lys Phe Arg Pro Glu Arg Phe Leu Ser Ser Glu His Ala His Val Asp 450 455 460 Phe Arg Gly Gln Gln His Glu Phe Met Pro Phe Gly Ser Gly Arg Arg 465 470 475 480 Met Cys Leu Gly Met Pro Leu Ala Ile Gly Val Val His Leu Thr Leu 485 490 495 Ala Arg Leu Leu His Glu Phe Glu Leu Ala Thr Pro Ser Asn Ala Pro 500 505 510 Val Asp Met Ser Glu Lys Ala Gly Leu Thr Leu Val Lys Ala Thr Pro 515 520 525 Leu Asn Val Leu Ile Ala Pro Arg Leu Cys 530 535 <210> 314 <211> 531 <212> PRT <213> Tinosporia cardofolia <400> 314 Met Asp Ser Ile Ile Leu Gln Ser Asn Leu Leu Thr Thr Val Ile Ile 1 5 10 15 Ile Thr Ser Phe Gly Leu Phe Val Leu Ser Phe Tyr Tyr Val Leu Trp 20 25 30 Lys Ala Ile Arg Thr Gly Asn Lys Arg Asn Cys Ser Asn Pro Lys Ala 35 40 45 Pro Glu Ala Ala Gly Gly Trp Pro Ile Leu Gly His Leu His Leu Phe 50 55 60 Arg Gly Gln Gly Leu Leu Leu His Lys Ile Phe Gly Ala Met Ala Glu 65 70 75 80 Lys His Gly Pro Ala Phe Thr Ile Arg Leu Gly Met Arg Pro Ala Leu 85 90 95 Val Val Ser Asn Trp Glu Val Ala Lys Glu Cys Phe Thr Ala Asn Asp 100 105 110 Arg Ala Leu Ala Ser Arg Pro Thr Ser Leu Ala Leu Lys Ile Met Gly 115 120 125 Tyr Asn Asn Ser Leu Phe Ala Phe Ala Pro Tyr Gly Gln Tyr Trp Arg 130 135 140 Glu Leu Arg Lys Ile Val Met His Gln Leu Leu Ser Ser Arg Arg Leu 145 150 155 160 Glu Leu Leu Lys Asn Val Trp Leu Ser Glu Ile Asp Ile Trp Ile Lys 165 170 175 Gly Leu Tyr Glu Asn Ser Leu Val Asn Lys Val Val Asp Met Lys Gln 180 185 190 Trp Phe Gly Glu Leu Met Met Asn Ile Val Val Arg Leu Val Ala Gly 195 200 205 Lys Arg Ser Phe Gly Lys Ile Arg Glu Gly Asp Ser Glu Glu Ala Lys 210 215 220 Ala His Gln Arg Gln Ile Lys Ala Leu Arg Asp Phe Phe Arg Leu Val 225 230 235 240 Glu Val Phe Met Ile Glu Asp Ala Phe Pro Phe Leu Thr Trp Leu Asp 245 250 255 Pro Arg Gly Tyr Gln Lys Glu Met Lys Asn Ile Ala Lys Glu Leu Asp 260 265 270 Tyr Leu Leu Glu Glu Trp Leu Glu Glu His Lys Leu Lys Gln Gln Ala 275 280 285 Ala Cys Asp Asp Gln Ser Lys Asp Phe Met Asp Val Met Leu Ser Glu 290 295 300 Val Lys Asp Ser Thr Ile Thr Glu Arg Asp Ala Asn Thr Ile Cys Lys 305 310 315 320 Ala Thr Cys Leu Asn Val Ile Leu Gly Ala Ser Asp Thr Thr Thr Leu 325 330 335 Thr Leu Thr Trp Ala Leu Ser Leu Leu Leu Asn Asn Arg His Val Leu 340 345 350 Lys Lys Ala Gln Glu Glu Leu Asn Ala Gln Val Gly Lys Asp Lys Gln 355 360 365 Val Glu Glu Ser Asp Met Lys Asn Leu Val Tyr Leu His Ala Ile Ile 370 375 380 Lys Glu Val Leu Arg Leu Tyr Pro Ala Ala Pro Leu Ser Val Ala His 385 390 395 400 Glu Ser Leu Glu Asp Thr Val Val Ala Gly Tyr His Val Ser Lys Gly 405 410 415 Thr Gln Val Ile Phe Asn Leu Trp Lys Ile Gln Arg Asp Pro Ser Val 420 425 430 Trp Ser Asn Pro Leu Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr His 435 440 445 Lys His Val Asp Ile Trp Gly Gln Asn Phe Glu Leu Ile Pro Phe Gly 450 455 460 Ser Gly Arg Arg Met Cys Pro Gly Val Pro Phe Ala Leu Gln Val Val 465 470 475 480 Ser Leu Thr Leu Ala Arg Leu Leu His Gly Phe Glu Leu Thr Thr Pro 485 490 495 Gly Gly Ala Ala Val Asp Met Thr Glu Thr Pro Gly Leu Thr Asn Met 500 505 510 Lys Ala Thr Pro Val Glu Val Phe Leu Arg Pro Asp Leu Pro His Gln 515 520 525 Leu Tyr Ala 530 <210> 315 <211> 524 <212> PRT <213> Thalictrum flavum <400> 315 Met Pro Ser Gln Thr Ile Gln Ile Ile Met Asp Phe Phe His Gln Cys 1 5 10 15 Val Ala Thr Ile Leu Phe Cys Val Leu Ala Ser Pro Phe Phe Phe Tyr 20 25 30 Tyr Leu Leu Pro Trp Trp Lys Asn Ala Asn Asp His Asp Leu Ala Lys 35 40 45 Ser Arg Gly Lys Pro Val Pro Glu Val Asp Gly Ala Trp Pro Ile Ile 50 55 60 Gly His Met His Met Leu Gln Pro Ser Asp Ser Phe Tyr Ser Ser Leu 65 70 75 80 Ala Glu Lys Tyr Gly Pro Ile Phe Thr Leu Arg Ile Gly Leu Cys Lys 85 90 95 Thr Ile Val Ile Asn Ser Trp Glu Leu Ala Lys Glu Cys Cys Thr Thr 100 105 110 His Asp Arg Val Phe Ala Ser Ser Pro Glu Ala Thr Ala Ala Lys Ile 115 120 125 Leu Gly Tyr Asp Tyr Ala Met Phe Gly Arg Asn Pro Tyr Gly Pro Tyr 130 135 140 Trp Arg Glu Met Arg Lys Ile Ile Ile Thr Glu Leu Val Ser Asn Arg 145 150 155 160 Arg Leu Glu Leu Leu Lys His Ile Arg Val Thr Glu Ile Ser Thr Ser 165 170 175 Ile Gln Glu Leu Tyr Gln Leu Trp Glu Ala Arg Ser Ser Lys Asn Val 180 185 190 Lys Glu Arg Val Val Val Asp Met Gln Lys Trp Phe Gly Asp Leu Met 195 200 205 Leu Asn Ile Gly Val Glu Met Val Ala Gly Lys Arg Tyr Phe Gly Ala 210 215 220 Ser Ser Asn Glu Asp Lys Glu Glu Ala Arg Gln Leu His Lys Thr Phe 225 230 235 240 Lys Asp Phe Ser Asn Leu Phe Gly Val Pro Val Leu Ser Asp Ala Ile 245 250 255 Pro Phe Leu Arg Trp Leu Asp Tyr Lys Gly His Ile Lys Ala Met Lys 260 265 270 Arg Cys Ala Lys Gln Val Asp Cys Ile Leu His Arg Trp Leu Glu Glu 275 280 285 His Lys Gln Asn Lys Thr Thr Ile Ala Gly Asp Phe Met Asp Ile Leu 290 295 300 Leu Ser Val Leu Gln Asp Lys Glu Ile Phe Gly Arg Asp Ala Asp Thr 305 310 315 320 Val Ile Lys Ala Thr Cys Leu Asn Met Met Leu Gly Val Val Glu Thr 325 330 335 Asn Lys Val Thr Val Thr Met Ala Leu Ile Ser Leu Leu Asn Asn Arg 340 345 350 Arg Ile Leu Lys Lys Ala Gln Glu Glu Ile Asp Ile His Val Gly Ser 355 360 365 Asp Lys Gln Val Glu Glu Ser Asp Ile Glu Lys Leu Val Tyr Leu Gln 370 375 380 Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr Ser Pro Gly Leu Gly Thr 385 390 395 400 Arg Glu Pro Ser Glu Asp Cys Thr Ile Ser Gly Phe His Val Pro Lys 405 410 415 Gly Thr Glu Val Met Val Asn Val Gln Gln Ile His Arg Asp Pro Ser 420 425 430 Ile Trp Ser Asn Pro Leu Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr 435 440 445 Gln Ala Asn Ile Asp Phe Gly Gly Gln His His Glu Tyr Ile Pro Phe 450 455 460 Gly Ala Gly Arg Arg Leu Cys Pro Ala Ile Ser Phe Ala Val Leu Val 465 470 475 480 Val His Leu Thr Leu Ala Arg Leu Leu Gln Gly Phe Asp Phe Ala Thr 485 490 495 Pro Lys Asp Ala Pro Val Glu Met Asn Glu Ser Val Glu Thr Leu Glu 500 505 510 Val Leu Ile Thr Pro Arg Leu Pro Leu His Met Tyr 515 520 <210> 316 <211> 534 <212> PRT <213> Xanthoriza simplicissima <400> 316 Met His Asn Pro Ile Pro Asn Gln Trp Leu Pro Ser Ser Thr Leu Leu 1 5 10 15 Thr Pro Ile Ile Val Ser Phe Leu Val Ile Val Leu Phe Tyr Ile Tyr 20 25 30 Lys Lys Arg Ser Ser Asn Thr Ile Arg Thr Lys Lys Ala Pro Glu Val 35 40 45 Val Gly Ala Trp Pro Val Ile Gly His Leu Asn Leu Phe Ser Cys Pro 50 55 60 Lys Pro Gly His Ile Val Leu Gly Glu Leu Ala Asp Gln Tyr Gly Pro 65 70 75 80 Ala Phe Thr Ile His Phe Gly Met His Pro Thr Leu Val Val Ser Ser 85 90 95 Ser Glu Leu Val Arg Asp Cys Phe Thr Thr Asn Asp Lys Leu Phe Ser 100 105 110 Ser Arg Leu Val Asn Lys Ala Ile Lys Tyr Met Phe Tyr Asp Gln Asp 115 120 125 Thr Ile Ser Phe Ala Pro Tyr Gly Pro Tyr Trp Arg Glu Leu Arg Lys 130 135 140 Met Ile Ala Leu Asn Leu Leu Asn Asn Glu Arg Ile Lys Met Leu Gln 145 150 155 160 Gln Gln Arg Ile Ser Glu Met Asp Ala Cys Leu Lys Lys Leu Tyr Asp 165 170 175 Leu Ser Ala Lys Arg Lys Asp Glu Asn Ala Gly Val Leu Val Asp Met 180 185 190 Ser Lys Trp Phe Ala Glu Ile Ser Phe Asn Val Val Thr Arg Ile Val 195 200 205 Ala Gly Lys His Ile Phe Gly Pro Lys Val Glu Arg Tyr Lys Asn Val 210 215 220 Met Glu Glu Ala Arg Arg Leu Met Asp Val Met Val Phe Ser Asp Met 225 230 235 240 Val Pro Tyr Leu Gly Trp Leu Asp Arg Leu Arg Gly Val Asp Ser Ala 245 250 255 Ile Lys Arg Thr Ala Lys Glu Leu Asp Ser Val Leu Glu Ser Trp Val 260 265 270 Glu Glu His Arg Arg Lys Ser Val Ser Ile Ser Ala Gly Thr Gly Gly 275 280 285 Ile Phe Asn Ile Lys Glu Glu Glu Glu Leu Asp Phe Ile Asp Ile Met 290 295 300 Leu Ser Ile Ile Ala Lys Asn Asn Leu Pro Gly Asp Asp Pro Asp Thr 305 310 315 320 Leu Ile Lys Ala Ile Val Gln Glu Thr Tyr Leu Ala Ala Trp Asp Asn 325 330 335 Thr Thr Val Thr Leu Thr Trp Val Leu Cys Leu Leu Leu Asn Asn Lys 340 345 350 Gln Val Leu Lys Arg Ala Gln Asn Glu Leu Asp Ala Gln Val Gly Lys 355 360 365 Glu Arg Gln Val Glu Asp Ser Asp Ile Asn Asn Leu Pro Tyr Val Gln 370 375 380 Ala Ile Val Lys Glu Ser Leu Arg Leu Tyr Pro Pro Gly Pro Ile Ile 385 390 395 400 Glu Arg Ala Thr Thr Glu Asp Cys Asn Val Gly Gly Phe Arg Val Pro 405 410 415 Ala Gly Thr Arg Leu Trp Val Asn Leu Trp Lys Leu Gln Arg Asp Pro 420 425 430 Lys Val Trp Pro Asn Asp Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu 435 440 445 Asn Asp Asn Ala Asp Ile Asp Leu Arg Gly Gln Asn Ser Glu Leu Ile 450 455 460 Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Val Ser Phe Ser Leu 465 470 475 480 Gln Val Ile His Leu Val Ile Ala Arg Ile Ile Gln Gly Phe Glu Leu 485 490 495 Lys Ala Pro Thr Asp Ala Asp Ile Asp Met Ser Thr Thr Leu Gly Met 500 505 510 Ile Ser Trp Lys Ala Thr Pro Leu Glu Val Leu Met Asn Pro Arg Phe 515 520 525 Pro Pro Val Phe Tyr Lys 530 <210> 317 <211> 550 <212> PRT <213> Xanthoriza simplicissima <400> 317 Met Ser Thr Leu Gln Ile Ser Ile Leu Thr Val Lys Ser Pro Tyr Ser 1 5 10 15 Gln Met His Tyr Tyr Pro Leu Leu His Gln Trp Leu Pro Ala Ser Met 20 25 30 Ala Leu Val Ser Leu Leu Val Ile Ile Leu Phe Ser Ile Phe Lys Lys 35 40 45 Arg Ser Ser Lys Met Ile Lys Ala Lys Lys Ala Pro Glu Val Ala Gly 50 55 60 Ala Trp Pro Leu Ile Gly His Leu Asn Leu Phe Ser Gly Pro Lys Leu 65 70 75 80 Leu His Leu Val Leu Gly Glu Leu Thr Asp Gln Tyr Gly Pro Ala Phe 85 90 95 Met Ile His Leu Gly Met Tyr Pro Thr Leu Val Val Ser Asn Trp Glu 100 105 110 Leu Leu Lys Asp Cys Phe Thr Thr Asn Asp Ile Phe Phe Ser Asn Arg 115 120 125 Pro Val Asn Lys Ala Ile Lys His Met Phe Tyr Asn Lys Glu Ser Ile 130 135 140 Gly Phe Thr Pro Tyr Gly Ser Tyr Trp Arg Glu Leu Arg Lys Met Thr 145 150 155 160 Thr Leu Lys Leu Leu Ser Asn His Arg Leu Asp Leu Leu Lys Pro Leu 165 170 175 Arg Ile Ser Glu Met Asp Ala Cys Phe Arg Asn Leu Tyr Glu Leu Trp 180 185 190 Thr Lys Asp Lys Asp Gly Asn Ala Trp Leu Leu Val Asp Met Ser Lys 195 200 205 Trp Phe Gly Glu Ile Ser Phe Asn Val Val Ala Arg Ile Val Ala Gly 210 215 220 Lys Lys Asn Phe Gly Ser Lys Gly Asp Arg Tyr Lys Thr Val Met Glu 225 230 235 240 Glu Val Val Arg Leu Met Gly Leu Arg Ala Leu Ser Asp Ala Val Pro 245 250 255 Tyr Leu Gly Trp Leu Asp Gln Leu Arg Gly Leu Asp Ser Ala Met Lys 260 265 270 Arg Ala Ala Lys Glu Leu Asp Ser Val Leu Glu Ser Trp Val Glu Glu 275 280 285 His Arg Leu Lys Arg Val Ser Val Ser Ala Gly Thr Gly Ser Thr Val 290 295 300 Lys Thr Ala Lys Glu Glu Glu Glu Glu Glu Asp Phe Ile Asp Ile Thr 305 310 315 320 Leu Ser Ile Leu Ala Glu Asn Gln Leu Pro Gly Asp Asp Pro Asp Thr 325 330 335 Gly Ile Lys Ser Leu Ile Leu Asp Met Ile Leu Gly Gly Ser Asp Thr 340 345 350 Ser Thr Val Thr Leu Ile Trp Ala Met Cys Leu Leu Leu Asn Asn Val 355 360 365 His Val Leu Lys Arg Ala Gln Tyr Glu Leu Asp Ala Gln Ile Gly Lys 370 375 380 Glu Arg Gln Val Glu Asp Ser Asp Ile Lys Asn Leu Pro Tyr Ile Gln 385 390 395 400 Ala Ile Ile Lys Glu Thr Met Arg Leu Tyr Pro Ala Gly Pro Ile Ile 405 410 415 Glu Arg Gln Ala Asn Glu Asp Cys Asp Val Gly Gly Phe His Val Pro 420 425 430 Ala Gly Thr Arg Leu Trp Val Asn Leu Trp Lys Leu Gln Arg Asp Pro 435 440 445 Asn Val Trp Lys Asp Asp Ala Leu Glu Phe Arg Pro Glu Arg Phe Leu 450 455 460 Thr Asp His Ala Asp Ile Asp Leu Lys Gly Gln His Leu Glu Leu Ile 465 470 475 480 Pro Phe Gly Ser Gly Arg Arg Ile Cys Pro Gly Ile Ser Phe Ala Leu 485 490 495 Gln Val Met His Leu Ala Leu Ala Arg Ile Ile His Gly Phe Glu Leu 500 505 510 Lys Thr Pro Asn Asp Ser Asn Ile Asp Met Ser Gly Thr Pro Gly Leu 515 520 525 Leu Cys Cys Lys Ala Thr Pro Leu Gln Val Leu Leu Thr Pro Arg Phe 530 535 540 Asn Pro Met Phe Tyr Lys 545 550 <210> 318 <211> 520 <212> PRT <213> Xanthoriza simplicissima <400> 318 Met Asp Ser Leu Arg His Cys Leu Gly Gly Ile Leu Ala Leu Leu Ile 1 5 10 15 Phe Leu Gly Tyr Leu Gln Trp Lys Arg Gly Thr Ser Asn Lys Cys Ile 20 25 30 Glu Ala Pro Gln Pro Asp Gly Ala Trp Pro Ile Ile Gly His Leu Pro 35 40 45 Arg Leu Met Lys Pro Gln Ile Met His Arg Thr Leu Ser Thr Met Ala 50 55 60 Asp Lys His Gly Pro Ala Phe Thr Leu Arg Leu Gly Val His Lys Thr 65 70 75 80 Leu Val Val Ser Ser Trp Glu Val Ala Lys Glu Cys Phe Thr Thr Asn 85 90 95 Asp Arg Val Phe Ala Thr Arg Pro Thr Ser Ile Ala Val Glu Ile Leu 100 105 110 Gly Tyr Asn Tyr Ala Leu Phe Ala Phe Gly Pro Tyr Gly Ser Tyr Trp 115 120 125 Arg Glu Ala Arg Lys Ile Ala Ile Leu Glu Leu Leu Ser Asn His Arg 130 135 140 Leu Glu Leu Leu Lys His Val Arg Ile Ser Glu Val Ser Thr Ser Ile 145 150 155 160 Arg Glu Leu Tyr Gln Val Trp Lys Glu Asn Cys Asp Ile Ala Asn Gly 165 170 175 Ser Ala Leu Val Asp Met Lys Pro Trp Phe Gly Asp Leu Thr Leu Asn 180 185 190 Val Val Val Arg Met Val Ala Gly Lys Arg Tyr Met Gly Gly Ser Val 195 200 205 Lys Ser Asp Asp Val Glu Gly Arg Arg Phe Gln Lys Ala Thr Lys Asp 210 215 220 Leu Phe Asp Leu Phe Gly Leu Phe Ile Leu Ser Asp Ala Leu Pro Phe 225 230 235 240 Met Gly Trp Leu Asp Leu His Gly His Lys Lys Ala Met Lys Lys Thr 245 250 255 Ala Lys Glu Leu Asp Ser Ile Met Gln Arg Trp Leu Glu Glu His Arg 260 265 270 Gln Ser Leu Leu Asp Asp Gly Thr Lys Glu Glu Lys Lys Asp Phe Met 275 280 285 Tyr Val Met Leu Ser Ile Leu Glu Asp Lys Lys Leu Phe Gln Tyr Asp 290 295 300 Ala Asp Ile Val Asn Lys Ala Ile Cys Met Asn Met Ile Met Ala Gly 305 310 315 320 Thr Asp Thr Gln Met Ile Thr Leu Thr Trp Val Leu Ser Leu Leu Leu 325 330 335 Asn Asn Arg His Ile Leu Lys Lys Ala Gln Asp Glu Ile Asp Ser Ser 340 345 350 Val Gly Lys Asp Arg Gln Val Glu Glu Ser Asp Ile Val Lys Leu Val 355 360 365 Tyr Leu Gln Ala Ile Val Lys Glu Ala Leu Arg Leu Tyr Pro Pro Ala 370 375 380 Pro Leu Ser Ala Gln His Glu Ala Met Glu Asp Cys Thr Val Ala Gly 385 390 395 400 Tyr Tyr Val Pro Ala Gly Thr Arg Leu Ile Thr Asn Ile Arg Lys Ile 405 410 415 Gln Arg Asp Pro Arg Val Trp Ser Asp Pro Phe Glu Phe His Pro Glu 420 425 430 Arg Phe Leu Thr Asn Gln Ala Asn Val Asp Phe Arg Gly Gln Ser Phe 435 440 445 Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Met Cys Pro Gly Ile Ser 450 455 460 Phe Ala Leu Gln Val Val His Leu Thr Leu Ala Arg Ile Leu Gln Gly 465 470 475 480 Phe Asp Phe Glu Thr Pro Met Asn Ala Ser Val Asp Met Thr Glu Ala 485 490 495 Pro Gly Leu Thr Asn Val Lys Ser Thr Pro Leu Gln Val Leu Ile Thr 500 505 510 Pro Arg Leu His Pro Asn Leu Tyr 515 520 <210> 319 <211> 523 <212> PRT <213> Xanthoriza simplicissima <400> 319 Met Asp Leu Leu Asn Pro Tyr Phe Ala Thr Ile Phe Gly Gly Phe Phe 1 5 10 15 Ala Leu Leu Ile Phe Leu Phe Ile Ile Ser Gln Lys Arg Ser Arg Ile 20 25 30 Pro Lys Ile Arg Ala Ala Pro Glu Pro Val Gly Ala Trp Pro Ile Val 35 40 45 Gly His Leu Pro Met Leu Leu Gly Pro Arg Leu Pro His Phe Val Leu 50 55 60 Gly Asp Leu Gly Glu Lys Tyr Gly Ser Ala Phe Thr Leu Arg Leu Gly 65 70 75 80 Ile His Lys Thr Leu Val Val Ser Ser Trp Glu Val Ala Lys Glu Cys 85 90 95 Phe Thr Thr Asn Asp Gln Val Phe Ala Thr Arg Pro Ser Phe Met Ala 100 105 110 Ala Lys Ile Met Gly Tyr Asn Tyr Ala Leu Phe Gly Leu Ala Pro Tyr 115 120 125 Gly Ser Tyr Trp Arg Glu Leu Arg Lys Ile Ser Ile Ile Glu Leu Leu 130 135 140 Ser Ser His Arg Leu Glu Leu Leu Lys His Val Arg Val Ser Glu Val 145 150 155 160 Ser Thr Ser Met Lys Glu Leu Tyr Glu Val Trp Ala Glu Asn Cys Ser 165 170 175 Asn Gly Asn Gly Ser Val Leu Val Glu Met Gln Arg Trp Phe Gly Asp 180 185 190 Leu Thr Leu Asn Ile Ser Val Arg Met Val Ala Gly Lys Arg Tyr Phe 195 200 205 Gly Thr Ser Ala Ser Leu Asp Asp Asp Glu Ala Arg Arg Ile Ser Lys 210 215 220 Ala Thr Lys Asp Phe Phe Arg Leu Thr Gly Met Phe Val Val Ser Asp 225 230 235 240 Ser Val Pro Phe Leu Trp Trp Leu Asp Phe Gln Gly His Glu Lys Ala 245 250 255 Met Lys Arg Thr Ala Lys Glu Met Asp Asp Ile Phe Gly Gly Trp Leu 260 265 270 Glu Asp His Arg Arg Asn Lys Leu Ala Gly Gly Thr Lys Val Gln Gln 275 280 285 Asp Phe Met Asp Val Met Leu Ser Ile Leu Glu Asp Glu Lys Phe Phe 290 295 300 Gly Tyr Asn Ala Asp Val Val Thr Lys Ala Thr Cys Leu Asn Met Leu 305 310 315 320 Leu Gly Gly Thr Asp Thr Thr Met Val Thr Leu Thr Trp Ala Leu Ser 325 330 335 Leu Leu Leu Asn Asn Arg His Ile Leu Lys Lys Ala Gln Thr Glu Ile 340 345 350 Asn Thr His Val Gly Lys Asp Thr Gln Val Asp Glu Ser Asp Ile Val 355 360 365 Lys Leu Val Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu Tyr 370 375 380 Pro Ala Ala Pro Leu Ser Thr Pro His Glu Ala Thr Lys Asp Cys Thr 385 390 395 400 Ile Ala Gly Tyr His Val Thr Ala Gly Thr Arg Leu Ile Thr Asn Ile 405 410 415 Trp Lys Ile Gln Arg Asp Pro Arg Val Trp Ser Asn Pro Ser Glu Phe 420 425 430 Gln Pro Glu Arg Phe Leu Thr Asp Gln Ala Asn Val Asp Val Arg Gly 435 440 445 Gln His Phe Glu Phe Ile Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro 450 455 460 Gly Ile Ser Leu Gly Leu Leu Val Val Gln Leu Ala Leu Ala Arg Leu 465 470 475 480 Leu Gln Gly Phe Asp Phe Glu Thr Pro Ser Asp Ala Leu Val Asp Met 485 490 495 Thr Glu Ser Ala Gly Leu Thr Asn Leu Lys Ala Thr Pro Leu His Val 500 505 510 Leu Ile Thr Pro Arg Leu His Ser Ser Leu Tyr 515 520 <210> 320 <211> 530 <212> PRT <213> Xanthoriza simplicissima <400> 320 Met Asp Ser Val His Gln Gly Gln Tyr Leu Pro Thr Pro Met Val Ser 1 5 10 15 Val Phe Val Phe Phe Ile Ser Leu Tyr Phe Leu Ile Val Trp Lys Thr 20 25 30 Arg Ser Ser Ser Lys Thr Asn Thr Cys Asn Glu Val Pro Glu Ala Pro 35 40 45 Gly Ala Trp Pro Ile Ile Gly His Leu His Leu Leu Gly Gly Ser Glu 50 55 60 Leu Pro His Lys Thr Leu Gly Ala Met Ala Asp Lys Tyr Gly Pro Ile 65 70 75 80 Phe Lys Ile Arg Ile Gly Val His Gln Ala Leu Val Val Ser Ser Ser 85 90 95 Asp Ile Val Lys Glu Cys Phe Thr Thr Asn Asp Lys Val Phe Ala Ser 100 105 110 Arg Pro Thr Ser Thr Ala Ser Lys Ile Leu Gly Tyr Asp Tyr Val Met 115 120 125 Phe Gly Phe Ser Pro Tyr Gly Pro Tyr Trp Ile Glu Leu Arg Lys Ile 130 135 140 Ile Met Ser Glu Leu Leu Ser Asn Arg Arg Leu Glu Leu Leu Lys His 145 150 155 160 Val Arg Asp Ser Glu Ile Asp Ile Ser Ile Gln Glu Leu Tyr Lys Val 165 170 175 Trp Lys Asn His Asp Lys Ser Lys Gly Pro Ile Leu Val Asp Met Lys 180 185 190 Gln Trp Phe Gly Asp Leu Ala Leu Asn Val Ile Leu Arg Met Ile Ala 195 200 205 Gly Lys Arg Tyr Ser Gly Ser Ile Phe Ser Ser Asp Glu Thr Glu Ala 210 215 220 Arg Arg Cys Gln Lys Gly Ala Arg Asp Phe Phe Arg Leu Leu Gly Leu 225 230 235 240 Phe Ile Ile Glu Asp Ala Leu Pro Tyr Leu Ser Trp Phe Asp Leu Gln 245 250 255 Gly Tyr Lys Lys Glu Met Lys Asn Thr Ala Lys Glu Leu Asp Ser Val 260 265 270 Phe Gln Arg Trp Leu Glu Glu His Lys Arg Thr Arg Glu Thr Gly Glu 275 280 285 Leu Asn Arg Glu Gln Asp Phe Met Asp Ile Leu Met Ser Thr Leu Glu 290 295 300 Glu Thr Lys Ile Ser Glu Tyr Asp Asn Asp Thr Ile Ile Lys Ser Thr 305 310 315 320 Cys Leu Ser Ile Val Thr Gly Gly Asn Asp Thr Thr Met Val Thr Leu 325 330 335 Thr Trp Ile Leu Ser Leu Leu Leu Asn Asn Lys His Ala Leu Lys Lys 340 345 350 Val Gln Asp Glu Leu Asp Ser His Val Gly Lys His Arg His Val Glu 355 360 365 Glu Ser Asp Ile Lys Asn Leu Ile Tyr Leu Gln Ala Ile Met Lys Glu 370 375 380 Ala Leu Arg Leu Tyr Pro Ala Gly Pro Leu Ser Gly Pro Arg Val Ala 385 390 395 400 Asp Ala Asp Cys Thr Leu Ala Gly Tyr His Ile Pro Ala Gly Thr Arg 405 410 415 Leu Leu Val Asn Thr Tyr Lys Ile Gln Arg Asp Pro Leu Val Trp Ser 420 425 430 Glu Pro Ser Glu Phe Arg Pro Glu Arg Phe Leu Thr Ser His Val Asn 435 440 445 Met Asp Val Lys Gly Leu Gln Tyr Glu Leu Ile Pro Phe Gly Thr Gly 450 455 460 Arg Arg Ala Cys Pro Gly Met Leu Phe Ala Leu Gln Val Val Pro Leu 465 470 475 480 Val Leu Ala Arg Phe Leu His Glu Phe Glu Pro Lys Thr Glu Met Asp 485 490 495 Met Pro Val Asp Met Thr Glu Thr Ala Gly Leu Ser Asn Ala Lys Ala 500 505 510 Thr Pro Leu Glu Val Val Ile Thr Pro Arg Leu Gln Pro Glu Leu Tyr 515 520 525 Ser Leu 530 <210> 321 <211> 528 <212> PRT <213> Xanthoriza simplicissima <400> 321 Met Asp Phe Thr Met Ile Ile Gln Trp Leu Leu Thr Ser Phe Ala Ile 1 5 10 15 Leu Val Ala Phe Val Leu Ile Trp Ser Ser Ala Thr Arg Ser Ser Thr 20 25 30 Arg Lys Arg Asn Lys Thr Ala Pro Val Ala Ala Gly Ala Leu Pro Ile 35 40 45 Val Gly His Leu His Met Leu Met Gly Arg Glu Leu Pro His Arg Leu 50 55 60 Leu Ser Asn Met Ala Asp Lys Tyr Gly Pro Ala Phe Met Met Lys Tyr 65 70 75 80 Gly Leu Gln Pro Ala Leu Val Val Ser Ser Leu Lys Leu Thr Lys Glu 85 90 95 Cys Phe Val His Asn Asp Arg Ala Val Phe Lys Arg Pro Arg Ser Lys 100 105 110 Ala Leu Lys Asn Leu Thr Tyr Asp Gln Ala Ser Leu Gly Phe Ala Pro 115 120 125 Tyr Gly Pro Leu Trp Val Glu Met Arg Lys Val Ser Lys Thr Asn Leu 130 135 140 Leu Ser Asn Gln Arg Leu Gln Met Gln Arg His Val Arg Ala Ser Glu 145 150 155 160 Val Asp Ala Phe Ile Lys Glu Leu Tyr Asp Leu Trp Ser Ser Lys Ser 165 170 175 Asn Asn Ile Lys Gly Ala Pro Leu Leu Val Glu Met Asn Lys Trp Phe 180 185 190 Glu Glu Leu Ala Leu Asn Val Val Thr Arg Met Leu Ser Gly Lys Arg 195 200 205 His Ile Gly Phe Lys Ala Arg Arg Gly Glu Asp Ser Glu Ser Met His 210 215 220 Tyr Lys Lys Val Val Val Asp Ala Thr Leu Leu Thr Ala Lys Leu Val 225 230 235 240 Val Ser Asp Phe Phe Pro Ser Leu Gly Leu Val Asp Tyr Leu Gln Gly 245 250 255 Asp Glu Ser Ser Ile Lys Gly Thr Ser Lys Glu Leu Asp Ala Ile Leu 260 265 270 Ala Thr Trp Val Glu Glu His Arg His Lys Lys Val Ser Gly Ser Glu 275 280 285 Asp Asp Glu Lys Asp Phe Ile Asp Leu Thr Leu Ser Met Ile Asp Gln 290 295 300 Thr Gln His Gln Gly Ala Asp Val Asp Thr Phe Ile Lys Ser Met Cys 305 310 315 320 Val Gly Met Ile Phe Gly Gly Ser Asp Ser Thr Ser Val Ala Leu Ala 325 330 335 Trp Ala Leu Ser Ile Leu Met Asn Asn Arg Pro Val Leu Lys Lys Ala 340 345 350 Arg Glu Glu Ile Asp Arg Gln Val Gly Arg Asp Arg Lys Val Asn Asp 355 360 365 Leu Asp Val Leu Lys Leu Asp Tyr Leu Lys Ala Ile Val Lys Glu Ser 370 375 380 Met Arg Leu Cys Leu Val Gly Pro Leu Leu Glu Arg Val Ala Val Glu 385 390 395 400 Asp Cys Glu Ile Gly Gly Tyr His Val Lys Ala Gly Ser Arg Val Val 405 410 415 Val Asn Ile Trp Lys Met Gln His Asp Pro Asn Leu Trp Ser Asp Pro 420 425 430 Leu Glu Phe Gln Pro Glu Arg Phe Leu Thr Thr Asn Ala Asn Val Glu 435 440 445 Leu Arg Gly Gln His Phe Glu Leu Leu Pro Phe Gly Ala Gly Ser Arg 450 455 460 Ile Cys Pro Gly Ile Thr Phe Ala Leu Glu Leu Ile Gln Leu Thr Leu 465 470 475 480 Ala Arg Leu Ile His Gly Phe Glu Leu Gly Thr Pro Met Asp Ala Asp 485 490 495 Val Asp Met Thr Glu Thr Ser Ser Val Thr Asn Tyr Arg Ala Thr Pro 500 505 510 Leu Glu Ile Leu Leu Thr Pro Arg Leu Asp Pro Lys Leu Tyr Asn Tyr 515 520 525 <210> 322 <211> 2703 <212> DNA <213> Papaver somniferum <400> 322 atggagctcc aatatatttc ttattttcaa ccaacttcct ccgttgttgc tcttctactt 60 gctcttgtat ccatcttatc cagtgtcgtt gttttgagga agacattttt gaataactac 120 tcatcatcac ctgcatcatc cacaaaaaca gcggtacttt ctcatcagcg gcagcagtcg 180 tgtgcattgc caatttccgg tctcctccat attttcatga ataaaaacgg cttaattcat 240 gtaactcttg gaaatatggc tgataaatac ggtccgattt tcagtttccc aacaggtagc 300 catagaactc tcgttgtgag cagttgggag atggtaaaag agtgttttac tggcaacaat 360 gacactgctt tctcaaaccg tcctatcccg ttagctttta agactatatt ctatgcatgc 420 ggtggcatag actcatacgg tctttcgagt gtaccttatg gaaaatattg gagggagctt 480 cgaaaggtct gtgtgcataa cctcctgtct aatcaacaac tactcaagtt cagacacttg 540 ataatttctc aagtcgatac gtctttcaat aagctgtatg agttatgcaa aaactctgaa 600 gacaaccagg gaaactatcc tactactact accgcagctg gcatggtgag aatcgatgat 660 tggctcgccg aattatcgtt caatgtgata ggaagaatag tctgcggatt ccaatcaggc 720 cctaagacag gtgctccaag cagggtggaa caatttaaag aagcaattaa tgaagcatct 780 tattttatgt cgacatctcc agtgtcagat aatgttccaa tgctagggtg gattgaccaa 840 ttgacaggtc ttacgagaaa tatgaagcac tgcggaaaga aattagactt ggtggtcgag 900 agcataatta atgatcatcg tcaaaagaga cgattctcta gaactaaagg aggagatgag 960 aaggacgatg aacaagatga cttcatcgac atttgtttgt caataatgga gcaaccacag 1020 cttcctggca acaataatcc ttctcagata cctatcaaat ctattgtcct ggacatgata 1080 ggtgggggca ctgacaccac aaaactgacc accatctgga ccctttcctt gctgctgaac 1140 aacccccatg tgttggacaa ggcaaaacaa gaagtggatg cacactttcg aaccaaaagg 1200 agatcaacaa atgatgcagc agcagccgtg gtggattttg atgatattcg taaccttgtc 1260 tacatccagg caatcatcaa agaatcaatg cggttgtatc cagccagccc cgtggtggag 1320 cgactgagcg gcgaagattg tgtggtcggt gggtttcatg taccagcagg gacgagatta 1380 tgggctaacg tatggaagat gcaacgagac cctaaagtat gggatgatcc attggtgttt 1440 cgaccagaca gatttttgag cgatgaacag aagatggttg atgtaagggg tcaaaattat 1500 gagctgttac catttggagc cggtcgacgt gtatgtccag gtgtatcctt ctctttggat 1560 ctaatgcaac tggtactgac tcgtcttatt ctcgagtttg aaatgaagtc tcctagcggg 1620 aaagtggaca tgacagcaac accaggatta atgagttaca aggtgatccc ccttgacatt 1680 ctgctcaccc atcgtcgcat aaagccgtgt gtgcagtcag cagcctctga gagagacatg 1740 gagagtagtg gtgtaccagt aatcactctg ggctcgggca aggtgatgcc tgttctgggc 1800 atgggaacat ttgagaaagt tggtaaaggg tccgaaagag agaggttggc gattttaaaa 1860 gcgatagagg tgggttacag atacttcgat acagctgctg catacgaaac tgaagaggtt 1920 cttggagaag ctattgctga agcacttcaa cttggcctag tcaaatctcg agatgaactt 1980 ttcatcagtt ccatgctctg gtgcactgat gctcacgctg atcgtgtcct cctcgctctt 2040 cagaattcgc tgaggaatct taaattggag tatgtggatc tatatatgtt acccttcccg 2100 gcaagcttga agcctgggaa gataacgatg gacataccag aggaagatat ttgtcgcatg 2160 gactacaggt ctgtatgggc agccatggaa gagtgtcaaa accttggctt cactaaatca 2220 atcggtgtta gcaatttctc ctgcaaaaag cttcaggaat tgatggcgac cgccaacatc 2280 cctccagctg tgaatcaagt ggagatgagc ccggctttcc aacaaaagaa gctgagagag 2340 tattgcaacg caaataatat attagtcagt gcaatctctg tactgggatc aaacggaacc 2400 ccatggggct ccaatgcagt tttgggttct gaggtgctta agaaaattgc tatggccaaa 2460 ggaaaatctg ttgctcaggt tagtatgaga tgggtttacg agcaaggcgc gagtcttgtg 2520 gtaaaaagtt tcagtgaaga gagattgagg gaaaacttga acatatttga ctgggaactc 2580 actaaggaag accatgaaaa gatcggtgag attccacagt gcagaatctt gagtgcttat 2640 tttttggtct cacctaatgg acctttcaaa tctcaagaag agttgtggga tgatgaagct 2700 tga 2703 <210> 323 <211> 900 <212> PRT <213> Papaver somniferum <400> 323 Met Glu Leu Gln Tyr Ile Ser Tyr Phe Gln Pro Thr Ser Ser Val Val 1 5 10 15 Ala Leu Leu Leu Ala Leu Val Ser Ile Leu Ser Ser Val Val Val Leu 20 25 30 Arg Lys Thr Phe Leu Asn Asn Tyr Ser Ser Ser Pro Ala Ser Ser Thr 35 40 45 Lys Thr Ala Val Leu Ser His Gln Arg Gln Gln Ser Cys Ala Leu Pro 50 55 60 Ile Ser Gly Leu Leu His Ile Phe Met Asn Lys Asn Gly Leu Ile His 65 70 75 80 Val Thr Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Ile Phe Ser Phe 85 90 95 Pro Thr Gly Ser His Arg Thr Leu Val Val Ser Ser Trp Glu Met Val 100 105 110 Lys Glu Cys Phe Thr Gly Asn Asn Asp Thr Ala Phe Ser Asn Arg Pro 115 120 125 Ile Pro Leu Ala Phe Lys Thr Ile Phe Tyr Ala Cys Gly Gly Ile Asp 130 135 140 Ser Tyr Gly Leu Ser Ser Val Pro Tyr Gly Lys Tyr Trp Arg Glu Leu 145 150 155 160 Arg Lys Val Cys Val His Asn Leu Leu Ser Asn Gln Gln Leu Leu Lys 165 170 175 Phe Arg His Leu Ile Ile Ser Gln Val Asp Thr Ser Phe Asn Lys Leu 180 185 190 Tyr Glu Leu Cys Lys Asn Ser Glu Asp Asn Gln Gly Asn Tyr Pro Thr 195 200 205 Thr Thr Thr Ala Ala Gly Met Val Arg Ile Asp Asp Trp Leu Ala Glu 210 215 220 Leu Ser Phe Asn Val Ile Gly Arg Ile Val Cys Gly Phe Gln Ser Gly 225 230 235 240 Pro Lys Thr Gly Ala Pro Ser Arg Val Glu Gln Phe Lys Glu Ala Ile 245 250 255 Asn Glu Ala Ser Tyr Phe Met Ser Thr Ser Pro Val Ser Asp Asn Val 260 265 270 Pro Met Leu Gly Trp Ile Asp Gln Leu Thr Gly Leu Thr Arg Asn Met 275 280 285 Lys His Cys Gly Lys Lys Leu Asp Leu Val Val Glu Ser Ile Ile Asn 290 295 300 Asp His Arg Gln Lys Arg Arg Phe Ser Arg Thr Lys Gly Gly Asp Glu 305 310 315 320 Lys Asp Asp Glu Gln Asp Asp Phe Ile Asp Ile Cys Leu Ser Ile Met 325 330 335 Glu Gln Pro Gln Leu Pro Gly Asn Asn Asn Pro Ser Gln Ile Pro Ile 340 345 350 Lys Ser Ile Val Leu Asp Met Ile Gly Gly Gly Thr Asp Thr Thr Lys 355 360 365 Leu Thr Thr Ile Trp Thr Leu Ser Leu Leu Leu Asn Asn Pro His Val 370 375 380 Leu Asp Lys Ala Lys Gln Glu Val Asp Ala His Phe Arg Thr Lys Arg 385 390 395 400 Arg Ser Thr Asn Asp Ala Ala Ala Ala Val Val Asp Phe Asp Asp Ile 405 410 415 Arg Asn Leu Val Tyr Ile Gln Ala Ile Ile Lys Glu Ser Met Arg Leu 420 425 430 Tyr Pro Ala Ser Pro Val Val Glu Arg Leu Ser Gly Glu Asp Cys Val 435 440 445 Val Gly Gly Phe His Val Pro Ala Gly Thr Arg Leu Trp Ala Asn Val 450 455 460 Trp Lys Met Gln Arg Asp Pro Lys Val Trp Asp Asp Pro Leu Val Phe 465 470 475 480 Arg Pro Asp Arg Phe Leu Ser Asp Glu Gln Lys Met Val Asp Val Arg 485 490 495 Gly Gln Asn Tyr Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys 500 505 510 Pro Gly Val Ser Phe Ser Leu Asp Leu Met Gln Leu Val Leu Thr Arg 515 520 525 Leu Ile Leu Glu Phe Glu Met Lys Ser Pro Ser Gly Lys Val Asp Met 530 535 540 Thr Ala Thr Pro Gly Leu Met Ser Tyr Lys Val Ile Pro Leu Asp Ile 545 550 555 560 Leu Leu Thr His Arg Arg Ile Lys Pro Cys Val Gln Ser Ala Ala Ser 565 570 575 Glu Arg Asp Met Glu Ser Ser Gly Val Pro Val Ile Thr Leu Gly Ser 580 585 590 Gly Lys Val Met Pro Val Leu Gly Met Gly Thr Phe Glu Lys Val Gly 595 600 605 Lys Gly Ser Glu Arg Glu Arg Leu Ala Ile Leu Lys Ala Ile Glu Val 610 615 620 Gly Tyr Arg Tyr Phe Asp Thr Ala Ala Ala Tyr Glu Thr Glu Glu Val 625 630 635 640 Leu Gly Glu Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Val Lys Ser 645 650 655 Arg Asp Glu Leu Phe Ile Ser Ser Met Leu Trp Cys Thr Asp Ala His 660 665 670 Ala Asp Arg Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys 675 680 685 Leu Glu Tyr Val Asp Leu Tyr Met Leu Pro Phe Pro Ala Ser Leu Lys 690 695 700 Pro Gly Lys Ile Thr Met Asp Ile Pro Glu Glu Asp Ile Cys Arg Met 705 710 715 720 Asp Tyr Arg Ser Val Trp Ala Ala Met Glu Glu Cys Gln Asn Leu Gly 725 730 735 Phe Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln 740 745 750 Glu Leu Met Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu 755 760 765 Met Ser Pro Ala Phe Gln Gln Lys Lys Leu Arg Glu Tyr Cys Asn Ala 770 775 780 Asn Asn Ile Leu Val Ser Ala Ile Ser Val Leu Gly Ser Asn Gly Thr 785 790 795 800 Pro Trp Gly Ser Asn Ala Val Leu Gly Ser Glu Val Leu Lys Lys Ile 805 810 815 Ala Met Ala Lys Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val 820 825 830 Tyr Glu Gln Gly Ala Ser Leu Val Val Lys Ser Phe Ser Glu Glu Arg 835 840 845 Leu Arg Glu Asn Leu Asn Ile Phe Asp Trp Glu Leu Thr Lys Glu Asp 850 855 860 His Glu Lys Ile Gly Glu Ile Pro Gln Cys Arg Ile Leu Ser Ala Tyr 865 870 875 880 Phe Leu Val Ser Pro Asn Gly Pro Phe Lys Ser Gln Glu Glu Leu Trp 885 890 895 Asp Asp Glu Ala 900 <210> 324 <211> 1473 <212> DNA <213> Papaver rhoeas <400> 324 atgatcggtc tttatcatgt tttcatgaat aaaaccggct taattcatgt aactcttgga 60 aacatggctg ataaatacgg acccattttc agtttcccaa caggtggcca tagggctcta 120 gttgtgagta gttgggagat ggcaaaagag tgttttacag gcgacaatga cattgttttt 180 tcaaatcgtc ctatgccgtt atcgtttaag attatattca atgcaggtgg tatagactca 240 gccggtctta cacaagtacc gtatggaaaa tattggaggg agttacgaaa gatctgtgtg 300 cacaaccttc tctcaaatca acaattactc aagttcaggc acttgataaa ttctcaagtt 360 gatacctctt tcactaaact gtacgagtca tgcaacaagg gaaattctgg catggtgaga 420 atggatgatt ggcttggaga attatcattc aacgtgatag gaagaatagt ctgcggattc 480 cgatcagaca ctgagacaag tgctacaagc agtgcggaac gatttacagt agcaattgat 540 gaagcatcgc gttttatgtc catacctgca gtgtcagata cctttccgtg gctaggatgg 600 attgatcgat taactggtct tataaaagat atgaagcacc atgcagaaaa attagactta 660 gtggttgaaa gcataattga agatcatcgt caaaagagac gattttctag aactaaaaaa 720 ggagatgaga ataacaccga ggatgaacaa gatgacttca ttgacatttg tttgtcaatt 780 atggagcaac cagagcttcc tggaaacaac tacgctcgtc aaatgcctat caaatctatc 840 atcgtggaca tgataggcgg ggcgactgac accacaaaac tgaccacaat ctggaccctt 900 tccttgctgc tgaataaccc acatgtgtta ggcaaggcaa aagaagaagt ggatgcacac 960 tttggaaaga aaaggagttc aaccaatggg gaagtcatgg tggattttga tgatattcgt 1020 agccttgtct acatccaggc aatcatcaaa gaatcaatgc ggttgtaccc agccagtcca 1080 gtggtggagc gactgagcag tgaagattgt gtggttggtg ggtttcatgt atcagcaggg 1140 acgcgattat gggttaacgt atggaaggtt caacgagacc cagaagtatg ggatgatccg 1200 tcggtgtttc gaccagagag atttttgagt aatgagcaga agatggttga tgtaaggggt 1260 caagattatg agttgttacc atttggagcc ggtcgacgga tatgtccagg tgtatcgttc 1320 tctttggatc taatgcacct ggtacttact cgtcttattc ttgagtttga aattaagtct 1380 cctggtgggg aagtggacat gacagcaaca caaggtttaa tgagttacaa ggtagtcccc 1440 cttgacattc tgctcacccg tcgcatgctt tag 1473 <210> 325 <211> 490 <212> PRT <213> Papaver rhoeas <400> 325 Met Ile Gly Leu Tyr His Val Phe Met Asn Lys Thr Gly Leu Ile His 1 5 10 15 Val Thr Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Ile Phe Ser Phe 20 25 30 Pro Thr Gly Gly His Arg Ala Leu Val Val Ser Ser Trp Glu Met Ala 35 40 45 Lys Glu Cys Phe Thr Gly Asp Asn Asp Ile Val Phe Ser Asn Arg Pro 50 55 60 Met Pro Leu Ser Phe Lys Ile Ile Phe Asn Ala Gly Gly Ile Asp Ser 65 70 75 80 Ala Gly Leu Thr Gln Val Pro Tyr Gly Lys Tyr Trp Arg Glu Leu Arg 85 90 95 Lys Ile Cys Val His Asn Leu Leu Ser Asn Gln Gln Leu Leu Lys Phe 100 105 110 Arg His Leu Ile Asn Ser Gln Val Asp Thr Ser Phe Thr Lys Leu Tyr 115 120 125 Glu Ser Cys Asn Lys Gly Asn Ser Gly Met Val Arg Met Asp Asp Trp 130 135 140 Leu Gly Glu Leu Ser Phe Asn Val Ile Gly Arg Ile Val Cys Gly Phe 145 150 155 160 Arg Ser Asp Thr Glu Thr Ser Ala Thr Ser Ser Ala Glu Arg Phe Thr 165 170 175 Val Ala Ile Asp Glu Ala Ser Arg Phe Met Ser Ile Pro Ala Val Ser 180 185 190 Asp Thr Phe Pro Trp Leu Gly Trp Ile Asp Arg Leu Thr Gly Leu Ile 195 200 205 Lys Asp Met Lys His His Ala Glu Lys Leu Asp Leu Val Val Glu Ser 210 215 220 Ile Ile Glu Asp His Arg Gln Lys Arg Arg Phe Ser Arg Thr Lys Lys 225 230 235 240 Gly Asp Glu Asn Asn Thr Glu Asp Glu Gln Asp Asp Phe Ile Asp Ile 245 250 255 Cys Leu Ser Ile Met Glu Gln Pro Glu Leu Pro Gly Asn Asn Tyr Ala 260 265 270 Arg Gln Met Pro Ile Lys Ser Ile Ile Val Asp Met Ile Gly Gly Ala 275 280 285 Thr Asp Thr Thr Lys Leu Thr Thr Ile Trp Thr Leu Ser Leu Leu Leu 290 295 300 Asn Asn Pro His Val Leu Gly Lys Ala Lys Glu Glu Val Asp Ala His 305 310 315 320 Phe Gly Lys Lys Arg Ser Ser Thr Asn Gly Glu Val Met Val Asp Phe 325 330 335 Asp Asp Ile Arg Ser Leu Val Tyr Ile Gln Ala Ile Ile Lys Glu Ser 340 345 350 Met Arg Leu Tyr Pro Ala Ser Pro Val Val Glu Arg Leu Ser Ser Glu 355 360 365 Asp Cys Val Val Gly Gly Phe His Val Ser Ala Gly Thr Arg Leu Trp 370 375 380 Val Asn Val Trp Lys Val Gln Arg Asp Pro Glu Val Trp Asp Asp Pro 385 390 395 400 Ser Val Phe Arg Pro Glu Arg Phe Leu Ser Asn Glu Gln Lys Met Val 405 410 415 Asp Val Arg Gly Gln Asp Tyr Glu Leu Leu Pro Phe Gly Ala Gly Arg 420 425 430 Arg Ile Cys Pro Gly Val Ser Phe Ser Leu Asp Leu Met His Leu Val 435 440 445 Leu Thr Arg Leu Ile Leu Glu Phe Glu Ile Lys Ser Pro Gly Gly Glu 450 455 460 Val Asp Met Thr Ala Thr Gln Gly Leu Met Ser Tyr Lys Val Val Pro 465 470 475 480 Leu Asp Ile Leu Leu Thr Arg Arg Met Leu 485 490 <210> 326 <211> 981 <212> DNA <213> Papaver rhoeas <400> 326 atggatagta gtggtgtacc tgtaatccct ctgagctccg gtaaagggat gcctgcttta 60 gctctaggaa catttgaaac atttggtaaa ggatcgtccg aaagacagag atcggcgttt 120 ttgaaagcga tagaggtggg ttacagatac tttgacacag gtgcttcata cggaaccgaa 180 gaggtccttg gcgaagctat agatgaagca cttcaacttg gccttatcga atctcgagag 240 gaactgttta tcagttccaa tctctggtgt actgatgctc accctgatcg tgtcctcctt 300 gctcttcaga attctctaag gaatcttaaa ttggagtatc tggatctata catgataccc 360 tttccgataa gcttgaagcc tgggaagata atggaggaga taactatgga cataccagag 420 gatgaaatgt tacctatgga ctacaagtct gtatgggcag ccatggaaga gtgtcaggac 480 cttggcttca ctaagtcgat cggtgtcagc aatttctcct gcaaaaagct tcaggagttg 540 atggcgaccg ccaatatccc accagctgtg aatcaagtgg agatgagccc cgttttccaa 600 caaaagaaac tgagagagta ttgcaaggcc aataatatat tagtcagcgc agtctcggtt 660 ttgggatcga acggaaccgc atggggctcc aatcaagtta tgggttctga ggtgttgaaa 720 caaattgcta ctgacagagg aaaatctgtt gctcagatta gtatgagatg ggtttatgag 780 caaggtgcga gtcttgtggt aaaaagtttc aatgaagtga ggatgaggga aaatttgaac 840 atattcaact ggcaacttac taaggaagat ttggaaaaga tcagcgagat tccacaatgc 900 aggatattaa ctggtgattt tttagtttca gctaatggac ctttcaaatc tctacaagag 960 ttatgggatg accaagtttg a 981 <210> 327 <211> 326 <212> PRT <213> Papaver rhoeas <400> 327 Met Asp Ser Ser Gly Val Pro Val Ile Pro Leu Ser Ser Gly Lys Gly 1 5 10 15 Met Pro Ala Leu Ala Leu Gly Thr Phe Glu Thr Phe Gly Lys Gly Ser 20 25 30 Ser Glu Arg Gln Arg Ser Ala Phe Leu Lys Ala Ile Glu Val Gly Tyr 35 40 45 Arg Tyr Phe Asp Thr Gly Ala Ser Tyr Gly Thr Glu Glu Val Leu Gly 50 55 60 Glu Ala Ile Asp Glu Ala Leu Gln Leu Gly Leu Ile Glu Ser Arg Glu 65 70 75 80 Glu Leu Phe Ile Ser Ser Asn Leu Trp Cys Thr Asp Ala His Pro Asp 85 90 95 Arg Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu 100 105 110 Tyr Leu Asp Leu Tyr Met Ile Pro Phe Pro Ile Ser Leu Lys Pro Gly 115 120 125 Lys Ile Met Glu Glu Ile Thr Met Asp Ile Pro Glu Asp Glu Met Leu 130 135 140 Pro Met Asp Tyr Lys Ser Val Trp Ala Ala Met Glu Glu Cys Gln Asp 145 150 155 160 Leu Gly Phe Thr Lys Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys 165 170 175 Leu Gln Glu Leu Met Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln 180 185 190 Val Glu Met Ser Pro Val Phe Gln Gln Lys Lys Leu Arg Glu Tyr Cys 195 200 205 Lys Ala Asn Asn Ile Leu Val Ser Ala Val Ser Val Leu Gly Ser Asn 210 215 220 Gly Thr Ala Trp Gly Ser Asn Gln Val Met Gly Ser Glu Val Leu Lys 225 230 235 240 Gln Ile Ala Thr Asp Arg Gly Lys Ser Val Ala Gln Ile Ser Met Arg 245 250 255 Trp Val Tyr Glu Gln Gly Ala Ser Leu Val Val Lys Ser Phe Asn Glu 260 265 270 Val Arg Met Arg Glu Asn Leu Asn Ile Phe Asn Trp Gln Leu Thr Lys 275 280 285 Glu Asp Leu Glu Lys Ile Ser Glu Ile Pro Gln Cys Arg Ile Leu Thr 290 295 300 Gly Asp Phe Leu Val Ser Ala Asn Gly Pro Phe Lys Ser Leu Gln Glu 305 310 315 320 Leu Trp Asp Asp Gln Val 325 <210> 328 <211> 966 <212> DNA <213> Papaver somniferum <400> 328 atggagagta atggtgtacc tatgatcact ctcagttccg gcattcggat gcctgcttta 60 ggtatgggaa cagctgaaac aatggtaaaa ggaacagaaa gagagaaatt ggcgtttttg 120 aaagcgatag aggtcggtta cagacacttc gatacagctg ctgcatacca aagtgaagag 180 tgtcttggtg aagctatagc tgaagcactt caacttggtc taataaaatc tcgagatgaa 240 ctcttcatca cttccaagct ctggtgcgct gatgctcacg ctgatcttgt cctccctgct 300 cttcagaatt ctctgaggaa tcttaaattg gactatcttg atctatattt gatacaccat 360 ccggtaagct tgaagccagg gaagtttgtt aacgaaatac caaaggatca tatccttcca 420 atggactaca aatctgtatg ggcagccatg gaagagtgtc agacccttgg cttcactagg 480 gcaatcgggg tctgtaattt ctcatgcaaa aagcttcaag agttgatggc agcagccaag 540 atccctccag ttgtgaatca agtggagatg agcccgactt tacatcaaaa aaatctgagg 600 gaatattgca aggccaataa tatcatgatc actgcacact cggttttggg agccatatgt 660 gctccatggg gcagcaatgc agttatggat tctaaggtgc ttcaccagat tgctgtggca 720 agaggaaaat ctgttgccca ggttagtatg agatgggttt accagcaagg cgcgagtcta 780 gtggtgaaaa gtttcaatga agggaggatg aaggaaaacc ttaagatatt tgattgggaa 840 ctaacggcag agaatatgga aaagatcagt gagattccgc aatctagaac aagctctgct 900 gatttcttgt tatcaccgac tggacctttc aaaactgaag aagagttctg ggatgagaag 960 gattga 966 <210> 329 <211> 321 <212> PRT <213> Papaver somniferum <400> 329 Met Glu Ser Asn Gly Val Pro Met Ile Thr Leu Ser Ser Gly Ile Arg 1 5 10 15 Met Pro Ala Leu Gly Met Gly Thr Ala Glu Thr Met Val Lys Gly Thr 20 25 30 Glu Arg Glu Lys Leu Ala Phe Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 His Phe Asp Thr Ala Ala Ala Tyr Gln Ser Glu Glu Cys Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Ile Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Thr Ser Lys Leu Trp Cys Ala Asp Ala His Ala Asp Leu 85 90 95 Val Leu Pro Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Asp Tyr 100 105 110 Leu Asp Leu Tyr Leu Ile His His Pro Val Ser Leu Lys Pro Gly Lys 115 120 125 Phe Val Asn Glu Ile Pro Lys Asp His Ile Leu Pro Met Asp Tyr Lys 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Thr Leu Gly Phe Thr Arg 145 150 155 160 Ala Ile Gly Val Cys Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu Met 165 170 175 Ala Ala Ala Lys Ile Pro Pro Val Val Asn Gln Val Glu Met Ser Pro 180 185 190 Thr Leu His Gln Lys Asn Leu Arg Glu Tyr Cys Lys Ala Asn Asn Ile 195 200 205 Met Ile Thr Ala His Ser Val Leu Gly Ala Ile Cys Ala Pro Trp Gly 210 215 220 Ser Asn Ala Val Met Asp Ser Lys Val Leu His Gln Ile Ala Val Ala 225 230 235 240 Arg Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Gln Gln 245 250 255 Gly Ala Ser Leu Val Val Lys Ser Phe Asn Glu Gly Arg Met Lys Glu 260 265 270 Asn Leu Lys Ile Phe Asp Trp Glu Leu Thr Ala Glu Asn Met Glu Lys 275 280 285 Ile Ser Glu Ile Pro Gln Ser Arg Thr Ser Ser Ala Asp Phe Leu Leu 290 295 300 Ser Pro Thr Gly Pro Phe Lys Thr Glu Glu Glu Phe Trp Asp Glu Lys 305 310 315 320 Asp <210> 330 <211> 321 <212> PRT <213> Papaver somniferum <400> 330 Met Glu Ser Ser Gly Val Pro Val Ile Thr Leu Gly Ser Gly Lys Val 1 5 10 15 Met Pro Val Leu Gly Met Gly Thr Phe Glu Lys Val Gly Lys Gly Ser 20 25 30 Glu Arg Glu Arg Leu Ala Ile Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 Tyr Phe Asp Thr Ala Ala Ala Tyr Glu Thr Glu Glu Val Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Val Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Ser Ser Met Leu Trp Cys Thr Asp Ala His Ala Asp Arg 85 90 95 Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Val Asp Leu Tyr Met Leu Pro Phe Pro Ala Ser Leu Lys Pro Gly Lys 115 120 125 Ile Thr Met Asp Ile Pro Glu Glu Asp Ile Cys Arg Met Asp Tyr Arg 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Asn Leu Gly Phe Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu Met 165 170 175 Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Ser Pro 180 185 190 Ala Phe Gln Gln Lys Lys Leu Arg Glu Tyr Cys Asn Ala Asn Asn Ile 195 200 205 Leu Val Ser Ala Ile Ser Val Leu Gly Ser Asn Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Leu Gly Ser Glu Val Leu Lys Lys Ile Ala Met Ala 225 230 235 240 Lys Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Glu Gln 245 250 255 Gly Ala Ser Leu Val Val Lys Ser Phe Ser Glu Glu Arg Leu Arg Glu 260 265 270 Asn Leu Asn Ile Phe Asp Trp Glu Leu Thr Lys Glu Asp His Glu Lys 275 280 285 Ile Gly Glu Ile Pro Gln Cys Arg Ile Leu Ser Ala Tyr Phe Leu Val 290 295 300 Ser Pro Asn Gly Pro Phe Lys Ser Gln Glu Glu Leu Trp Asp Asp Glu 305 310 315 320 Ala <210> 331 <211> 26 <212> DNA <213> Papaver somniferum <400> 331 ggatcccatc agttccatgc tctggt 26 <210> 332 <211> 26 <212> DNA <213> Papaver somniferum <400> 332 ggtaccgggc tcatctccac ttgatt 26 <210> 333 <211> 26 <212> DNA <213> Papaver somniferum <400> 333 ggatcccatc acttccaagc tctggt 26 <210> 334 <211> 25 <212> DNA <213> Papaver somniferum <400> 334 ggtaccgggc tcatctccac ttgat 25 <210> 335 <211> 34 <212> DNA <213> Papaver somniferum <400> 335 gaattcccta catactgtat tgggttgaat catg 34 <210> 336 <211> 25 <212> DNA <213> Papaver somniferum <400> 336 ggtacctaac gggataggac ggttt 25 <210> 337 <211> 1704 <212> DNA <213> Papaver somniferum <400> 337 atggagctcc aatatatttc ttattttcaa ccaacttcct ccgttgttgc tcttctactt 60 gctcttgtat ccatcttatc cagtgtcgtt gttttgagga agacattttt gaataactac 120 tcatcatcac ctgcatcatc cacaaaaaca gcggtacttt ctcatcagcg gcagcagtcg 180 tgtgcattgc caatttccgg tctcctccat attttcatga ataaaaacgg cttaattcat 240 gtaactcttg gaaatatggc tgataaatac ggtccgattt tcagtttccc aacaggtagc 300 catagaactc tcgttgtgag cagttgggag atggtaaaag agtgttttac tggcaacaat 360 gacactgctt tctcaaaccg tcctatcccg ttagctttta agactatatt ctatgcatgc 420 ggtggcatag actcatacgg tctttcgagt gtaccttatg gaaaatattg gagggagctt 480 cgaaaggtct gtgtgcataa cctcctgtct aatcaacaac tactcaagtt cagacacttg 540 ataatttctc aagtcgatac gtctttcaat aagctgtatg agttatgcaa aaactctgaa 600 gacaaccagg gaaactatcc tactactact accgcagctg gcatggtgag aatcgatgat 660 tggctcgccg aattatcgtt caatgtgata ggaagaatag tctgcggatt ccaatcaggc 720 cctaagacag gtgctccaag cagggtggaa caatttaaag aagcaattaa tgaagcatct 780 tattttatgt cgacatctcc agtgtcagat aatgttccaa tgctagggtg gattgaccaa 840 ttgacaggtc ttacgagaaa tatgaagcac tgcggaaaga aattagactt ggtggtcgag 900 agcataatta atgatcatcg tcaaaagaga cgattctcta gaactaaagg aggagatgag 960 aaggacgatg aacaagatga cttcatcgac atttgtttgt caataatgga gcaaccacag 1020 cttcctggca acaataatcc ttctcagata cctatcaaat ctattgtcct ggacatgata 1080 ggtgggggca ctgacaccac aaaactgacc accatctgga ccctttcctt gctgctgaac 1140 aacccccatg tgttggacaa ggcaaaacaa gaagtggatg cacactttcg aaccaaaagg 1200 agatcaacaa atgatgcagc agcagccgtg gtggattttg atgatattcg taaccttgtc 1260 tacatccagg caatcatcaa agaatcaatg cggttgtatc cagccagccc cgtggtggag 1320 cgactgagcg gcgaagattg tgtggtcggt gggtttcatg taccagcagg gacgagatta 1380 tgggctaacg tatggaagat gcaacgagac cctaaagtat gggatgatcc attggtgttt 1440 cgaccagaca gatttttgag cgatgaacag aagatggttg atgtaagggg tcaaaattat 1500 gagctgttac catttggagc cggtcgacgt gtatgtccag gtgtatcctt ctctttggat 1560 ctaatgcaac tggtactgac tcgtcttatt ctcgagtttg aaatgaagtc tcctagcggg 1620 aaagtggaca tgacagcaac accaggatta atgagttaca aggtgatccc ccttgacatt 1680 ctgctcaccc atcgtcgcat aaag 1704 <210> 338 <211> 568 <212> PRT <213> Papaver somniferum <400> 338 Met Glu Leu Gln Tyr Ile Ser Tyr Phe Gln Pro Thr Ser Ser Val Val 1 5 10 15 Ala Leu Leu Leu Ala Leu Val Ser Ile Leu Ser Ser Val Val Val Leu 20 25 30 Arg Lys Thr Phe Leu Asn Asn Tyr Ser Ser Ser Pro Ala Ser Ser Thr 35 40 45 Lys Thr Ala Val Leu Ser His Gln Arg Gln Gln Ser Cys Ala Leu Pro 50 55 60 Ile Ser Gly Leu Leu His Ile Phe Met Asn Lys Asn Gly Leu Ile His 65 70 75 80 Val Thr Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Ile Phe Ser Phe 85 90 95 Pro Thr Gly Ser His Arg Thr Leu Val Val Ser Ser Trp Glu Met Val 100 105 110 Lys Glu Cys Phe Thr Gly Asn Asn Asp Thr Ala Phe Ser Asn Arg Pro 115 120 125 Ile Pro Leu Ala Phe Lys Thr Ile Phe Tyr Ala Cys Gly Gly Ile Asp 130 135 140 Ser Tyr Gly Leu Ser Ser Val Pro Tyr Gly Lys Tyr Trp Arg Glu Leu 145 150 155 160 Arg Lys Val Cys Val His Asn Leu Leu Ser Asn Gln Gln Leu Leu Lys 165 170 175 Phe Arg His Leu Ile Ile Ser Gln Val Asp Thr Ser Phe Asn Lys Leu 180 185 190 Tyr Glu Leu Cys Lys Asn Ser Glu Asp Asn Gln Gly Asn Tyr Pro Thr 195 200 205 Thr Thr Thr Ala Ala Gly Met Val Arg Ile Asp Asp Trp Leu Ala Glu 210 215 220 Leu Ser Phe Asn Val Ile Gly Arg Ile Val Cys Gly Phe Gln Ser Gly 225 230 235 240 Pro Lys Thr Gly Ala Pro Ser Arg Val Glu Gln Phe Lys Glu Ala Ile 245 250 255 Asn Glu Ala Ser Tyr Phe Met Ser Thr Ser Pro Val Ser Asp Asn Val 260 265 270 Pro Met Leu Gly Trp Ile Asp Gln Leu Thr Gly Leu Thr Arg Asn Met 275 280 285 Lys His Cys Gly Lys Lys Leu Asp Leu Val Val Glu Ser Ile Ile Asn 290 295 300 Asp His Arg Gln Lys Arg Arg Phe Ser Arg Thr Lys Gly Gly Asp Glu 305 310 315 320 Lys Asp Asp Glu Gln Asp Asp Phe Ile Asp Ile Cys Leu Ser Ile Met 325 330 335 Glu Gln Pro Gln Leu Pro Gly Asn Asn Asn Pro Ser Gln Ile Pro Ile 340 345 350 Lys Ser Ile Val Leu Asp Met Ile Gly Gly Gly Thr Asp Thr Thr Lys 355 360 365 Leu Thr Thr Ile Trp Thr Leu Ser Leu Leu Leu Asn Asn Pro His Val 370 375 380 Leu Asp Lys Ala Lys Gln Glu Val Asp Ala His Phe Arg Thr Lys Arg 385 390 395 400 Arg Ser Thr Asn Asp Ala Ala Ala Ala Val Val Asp Phe Asp Asp Ile 405 410 415 Arg Asn Leu Val Tyr Ile Gln Ala Ile Ile Lys Glu Ser Met Arg Leu 420 425 430 Tyr Pro Ala Ser Pro Val Val Glu Arg Leu Ser Gly Glu Asp Cys Val 435 440 445 Val Gly Gly Phe His Val Pro Ala Gly Thr Arg Leu Trp Ala Asn Val 450 455 460 Trp Lys Met Gln Arg Asp Pro Lys Val Trp Asp Asp Pro Leu Val Phe 465 470 475 480 Arg Pro Asp Arg Phe Leu Ser Asp Glu Gln Lys Met Val Asp Val Arg 485 490 495 Gly Gln Asn Tyr Glu Leu Leu Pro Phe Gly Ala Gly Arg Arg Val Cys 500 505 510 Pro Gly Val Ser Phe Ser Leu Asp Leu Met Gln Leu Val Leu Thr Arg 515 520 525 Leu Ile Leu Glu Phe Glu Met Lys Ser Pro Ser Gly Lys Val Asp Met 530 535 540 Thr Ala Thr Pro Gly Leu Met Ser Tyr Lys Val Ile Pro Leu Asp Ile 545 550 555 560 Leu Leu Thr His Arg Arg Ile Lys 565 <210> 339 <211> 966 <212> DNA <213> Papaver somniferum <400> 339 atggagagta atggtgtacc tatgatcact ctcagttccg gcattcggat gcctgcttta 60 ggtatgggaa cagctgaaac aatggtaaaa ggaacagaaa gagagaaatt ggcgtttttg 120 aaagcgatag aggtcggtta cagacacttc gatacagctg ctgcatacca aactgaagag 180 tgtcttggtg aagctatagc tgaagcactt caacttggtc taataaaatc tcgagatgaa 240 ctcttcatca cttccaagct ctggtgcgct gatgctcacg ctgatcttgt cctccctgct 300 cttcagaatt ctctgaggaa tcttaaattg gactatcttg atctatattt gatacaccat 360 ccggtaagct tgaagccagg gaagtttgtt aacgaaatac caaaggatca tatccttcca 420 atggactaca aatctgtatg ggcagccatg gaagagtgtc agacccttgg cttcactagg 480 gcaatcgggg tctgtaattt ctcatgcaaa aggcttcaag agttgatgga aacagccaac 540 agccctccag ttgtgaatca agtggagatg agcccgactt tacatcaaaa aaatctgagg 600 gaatattgca aggccaataa tatcatgatc accgcacact cagttttggg agccgtaggt 660 gccgcctggg gcaccaatgc agttatgcat tctaaggtgc ttcaccagat tgctgtggcc 720 agaggaaaat ctgttgccca ggttagtatg agatgggttt accagcaagg cgcgagtctt 780 gtggtgaaaa gtttcaatga agcgaggatg aaggaaaacc ttaagatatt tgattgggaa 840 ctaacggcag aagacatgga aaagatcagt gagattccac aatctagaac aagctctgct 900 gctttcttgt tatcaccgac tggacctttc aaaactgaag aagagttctg ggatgagaag 960 gattga 966 <210> 340 <211> 321 <212> PRT <213> Papaver somniferum <400> 340 Met Glu Ser Ser Gly Val Pro Val Ile Thr Leu Gly Ser Gly Lys Val 1 5 10 15 Met Pro Val Leu Gly Met Gly Thr Phe Glu Lys Val Gly Lys Gly Ser 20 25 30 Glu Arg Glu Arg Leu Ala Ile Leu Lys Ala Ile Glu Val Gly Tyr Arg 35 40 45 Tyr Phe Asp Thr Ala Ala Ala Tyr Glu Thr Glu Glu Val Leu Gly Glu 50 55 60 Ala Ile Ala Glu Ala Leu Gln Leu Gly Leu Val Lys Ser Arg Asp Glu 65 70 75 80 Leu Phe Ile Ser Ser Met Leu Trp Cys Thr Asp Ala His Ala Asp Arg 85 90 95 Val Leu Leu Ala Leu Gln Asn Ser Leu Arg Asn Leu Lys Leu Glu Tyr 100 105 110 Val Asp Leu Tyr Met Leu Pro Phe Pro Ala Ser Leu Lys Pro Gly Lys 115 120 125 Ile Thr Met Asp Ile Pro Glu Glu Asp Ile Cys Arg Met Asp Tyr Arg 130 135 140 Ser Val Trp Ala Ala Met Glu Glu Cys Gln Asn Leu Gly Phe Thr Lys 145 150 155 160 Ser Ile Gly Val Ser Asn Phe Ser Cys Lys Lys Leu Gln Glu Leu Met 165 170 175 Ala Thr Ala Asn Ile Pro Pro Ala Val Asn Gln Val Glu Met Ser Pro 180 185 190 Ala Phe Gln Gln Lys Lys Leu Arg Glu Tyr Cys Asn Ala Asn Asn Ile 195 200 205 Leu Val Ser Ala Ile Ser Val Leu Gly Ser Asn Gly Thr Pro Trp Gly 210 215 220 Ser Asn Ala Val Leu Gly Ser Glu Val Leu Lys Lys Ile Ala Met Ala 225 230 235 240 Lys Gly Lys Ser Val Ala Gln Val Ser Met Arg Trp Val Tyr Glu Gln 245 250 255 Gly Ala Ser Leu Val Val Lys Ser Phe Ser Glu Glu Arg Leu Arg Glu 260 265 270 Asn Leu Asn Ile Phe Asp Trp Glu Leu Thr Lys Glu Asp His Glu Lys 275 280 285 Ile Gly Glu Ile Pro Gln Cys Arg Ile Leu Ser Ala Tyr Phe Leu Val 290 295 300 Ser Pro Asn Gly Pro Phe Lys Ser Gln Glu Glu Leu Trp Asp Asp Glu 305 310 315 320 Ala

Claims (84)

  1. (R)-레티큘린 또는 (R)-레티큘린의 전구체를 제조하는 방법으로서,
    (a) 벤질이소퀴놀린 유도체를 (R)-레티큘린 또는 (R)-레티큘린 전구체로 변환시킬 수 있는 이종(heterologous) 효소를 발현하는 미생물과 벤질이소퀴놀린 유도체를 접촉시키는 단계로서, 상기 효소는 시토크롬 P450(CYP450) 및 알도-케토 환원효소(AKR)의 융합 단백질이고 서열 번호 323과 적어도 85% 동일한 아미노산 서열로 이루어진 단계; 및
    (b) 상기 미생물을 성장시켜 (R)-레티큘린 또는 (R)-레티큘린의 전구체를 생성하는 단계를 포함하는, 방법.
  2. (R)-레티큘린 또는 (R)-레티큘린 전구체를 제조하는 방법으로서,
    (a) (i) 시토크롬 P450(CYP450) 폴리펩티드를 암호화하는 제1 핵산 서열;
    (ii) 알도-케토 환원효소(AKR) 폴리펩티드를 암호화하는 제2 핵산 서열; 및
    (iii) 숙주 세포에서 발현을 제어할 수 있는 하나 이상의 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하는 키메라 핵산 서열을 제공하는 단계;
    (b) 상기 키메라 핵산 서열을 상기 숙주 세포 내에 도입시키고 상기 숙주 세포를 성장시켜 CYP450 및 AKR을 생성하고 (R)-레티큘린 또는 (R)-레티큘린 전구체를 생성하는 단계로서, 상기 CYP450 및 AKR는 서열 번호 323과 적어도 85% 동일한 아미노산 서열로 이루어진 융합 단백질을 형성하는 단계; 및
    (c) (R)-레티큘린 또는 (R)-레티큘린 전구체를 회수하는 단계를 포함하는, 방법.
  3. (i) 숙주 세포에서 발현을 제어할 수 있는 핵산 서열; 및
    (ii) 시토크롬 P450(CYP450) 및 알도-케토 환원효소(AKR)을 암호화하는 핵산 서열을, 작동 가능하게 연결된 구성요소로서 포함하고,
    상기 CYP450 및 AKR은 서열 번호 323과 적어도 85% 동일한 아미노산 서열로 이루어진 융합 단백질을 형성하는, 숙주 세포에서의 발현에 적합한 재조합 발현 벡터.
  4. 제1항에 있어서, 상기 방법은 (R)-레티큘린을 제조하는, 방법.
  5. 제1항에 있어서, 상기 미생물은 효모인, 방법.
  6. 제1항에 있어서, 상기 CYP450 및 AKR의 융합 단백질은 서열 번호 323과 적어도 95% 동일한 아미노산 서열로 이루어진, 방법.
  7. 제1항에 있어서, 상기 (R)-레티큘린 또는 (R)-레티큘린 전구체를 살루타리딘, 살루타리딘-7-O-아세테이트, 또는 테바인으로 변환시키는 단계를 더 포함하는, 방법.
  8. 제2항에 있어서, 상기 숙주 세포는 식물 세포, 박테리아, 또는 효모인, 방법.
  9. 제2항에 있어서, 상기 방법은 (R)-레티큘린을 제조하는, 방법.
  10. 제2항에 있어서, 상기 (R)-레티큘린 또는 (R)-레티큘린 전구체를 살루타리딘, 살루타리딘-7-O-아세테이트, 또는 테바인으로 변환시키는 단계를 더 포함하는, 방법.
  11. 제3항에 있어서, 상기 숙주 세포는 식물 세포, 박테리아, 또는 효모인, 재조합 발현 벡터.
  12. 제2항에 있어서, 상기 CYP450 및 AKR의 융합 단백질은 서열 번호 323과 적어도 95% 동일한 아미노산 서열로 이루어진, 방법.
  13. 제2항에 있어서, 상기 숙주 세포는 효모인, 방법.
  14. 제3항에 있어서, 상기 CYP450 및 AKR의 융합 단백질은 서열 번호 323과 적어도 95% 동일한 아미노산 서열로 이루어진, 재조합 발현 벡터.
  15. 제3항에 있어서, 상기 숙주 세포는 효모인, 재조합 발현 벡터.
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