KR102003759B1 - Novel Salmonella specific bacteriophage SH9 and antibacterial composition comprising the same - Google Patents

Novel Salmonella specific bacteriophage SH9 and antibacterial composition comprising the same Download PDF

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KR102003759B1
KR102003759B1 KR1020170176343A KR20170176343A KR102003759B1 KR 102003759 B1 KR102003759 B1 KR 102003759B1 KR 1020170176343 A KR1020170176343 A KR 1020170176343A KR 20170176343 A KR20170176343 A KR 20170176343A KR 102003759 B1 KR102003759 B1 KR 102003759B1
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김선진
김영주
연영은
한범구
김현일
박철세
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Abstract

본 발명은 살모넬라균, 특히 살모넬라 하다(Salmonella Hadar)에 특이적인 사멸능을 갖는 신규 박테리오파지 SH9, 상기 박테리오파지를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다. 본 발명의 박테리오파지 SH9을 이용한 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대한 특이성이 매우 높고, 상기 박테리오파지 SH9은 살모넬라균에 감염하여 살모넬라균 내에서 100배 이상 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 SH9은 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 SH9은 살모넬라균, 특히 살모넬라 하다에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.The present invention relates to a novel bacteriophage SH9 having saliva specific to salmonella, particularly Salmonella Hadar, a pharmaceutical composition for preventing or treating infectious diseases caused by Salmonella including the bacteriophage, antibiotic composition, feed additive The present invention relates to a method for preventing or treating an infectious disease caused by Salmonella comprising the step of administering a composition, a feed, a disinfectant or a detergent, and the bacteriophage to an individual. The method for preventing or treating infectious diseases caused by Salmonella using the bacteriophage SH9 of the present invention is highly specific to Salmonella than the conventional chemicals such as antibiotics, and the bacteriophage SH9 is infected with Salmonella, It has an advantage that it is strong against the physicochemical stimulus with excellent lytic activity by proliferating more than 100 times. In addition, the bacteriophage SH9 of the present invention is not infected with any host other than bacteria such as human, animal, and plant. Therefore, it is possible to solve the problems of antibiotic resistant bacteria caused by misuse of antibiotics, have. Therefore, the bacteriophage SH9 of the present invention can be variously used in the field of preventing or treating infectious diseases caused by Salmonella, especially Salmonella, antibiotic composition, composition for feed addition, feed, disinfectant, or detergent.

Description

신규한 살모넬라균 특이 박테리오파지 SH9 및 이를 포함하는 항균 조성물{Novel Salmonella specific bacteriophage SH9 and antibacterial composition comprising the same}A novel Salmonella-specific bacteriophage SH9 and an antimicrobial composition comprising the same, which comprises the novel Salmonella specific bacteriophage SH9 and an antibacterial composition,

본 발명은 살모넬라균, 특히 살모넬라 하다(Salmonella Hadar)에 특이적인 사멸능을 갖는 신규 박테리오파지 SH9, 상기 박테리오파지를 포함하는 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제 및 상기 박테리오파지를 개체에 투여하는 단계를 포함하는 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법에 관한 것이다.The present invention relates to a novel bacteriophage SH9 having saliva specific to salmonella, particularly Salmonella Hadar, an antibiotic composition containing the bacteriophage, a composition for feed addition, a feed, a disinfectant or a cleansing agent, and the bacteriophage To a method for preventing or treating an infectious disease caused by Salmonella.

살모넬라(Salmonella)는 장내세균과에 속하는 그람음성의 통성혐기성 세균으로, 아포를 형성하지 않는 간균이다. 살모넬라균은 사람뿐만 아니라 돼지, 소, 닭 등을 비롯한 다양한 가축에 감염되어 질병을 유발하는데, 사람에게 감염되어 식중독을 유발하고 동물에게 있어서는 다양한 살모넬라증(Salmonellosis)을 유발하는 병원성 미생물이다. 살모넬라 엔테리카(Salmonella enterica)는 혈청학적 구분에 의해 살모넬라 하다(Salmonella Hadar), 장티푸스를 일으키는 살모넬라 타이피(Salmonella Typhi), 쥐티푸스의 원인균인 살모넬라 티피뮤리움(Salmonella Typhimurium), 장염균인 살모넬라 엔테리티디스(Salmonella Enteritidis), 가금티푸스의 원인균인 살모넬라 갈리나룸(Salmonella Gallinarum), 추백리 원인균인 살모넬라 플로럼(Salmonella Pullorum) 등의 혈청종을 포함한다. 살모넬라균은 혈청형에 따라 인간뿐만 아니라 가축에게도 질병을 일으킬 수 있는 인수공통 감염원을 포함한다. 살모넬라 티피뮤리움과 살모넬라 엔테리티디스는 인수공통 감염원으로 사람에게 있어서는 식중독의 원인이 되며, 소, 닭, 돼지 등의 가축에 있어서는 살모넬라증(salmonellosis)을 일으켜 급성 또는 만성 장염과 패혈증, 더 나아가 폐렴, 관절염, 유산 등을 발생시킨다. 살모넬라 하다는 가금류를 매체로 인간에 감염하며, 가금의 양식, 도살, 가공의 전 과정에 있어 직간접적으로 오염 기회가 주어진다. 또한 살모넬라 하다는 호텔이나 병원 등 많은 사람이 이용하는 건물 내에서 전염되기도 하는데, 영국 내 산부인과에서 산모와 아기간의 수직 전염이나 아기와 아기간의 수평 전염이 확인된 바 있다. 살모넬라 하다에 감염되면 일반적인 살모넬라증에서 나타나는 것처럼 설사와 같은 증상을 보일 수도 있으나, 이러한 병적 증상을 보이지 않는 경우도 있고, 설사 증상을 보이는 모든 환자가 살모넬라 하다에 감염된 것은 아니기 때문에 감염 여부를 파악하기 위해서는 균의 배양 및 혈청종 동정 과정이 필요하다. 살모넬라 하다는 다제 내성균으로써 앰피실린, 테트라사이클린, 날리딕스산에 내성을 지님이 밝혀졌고, 프랑스에서는 아목시실린과 시프로플록사신에 대해서도 내성을 지닌다는 것이 밝혀졌다. 이러한 살모넬라 하다의 위험성에도 불구하고 국내에서는 살모넬라 하다 혈청형에 대한 정확한 검사가 이루어지지 않고 있어 살모넬라 하다에 특정된 살모넬라증 사례가 미흡하여 외국에 비해 살모넬라 하다 감염의 예방 및 치료를 위한 연구가 활발히 이루어지지 않고 있는 실정이다. Salmonella (Salmonella) is a facultative anaerobic bacteria belonging to gram-negative enteric bacteria and a bacillus which does not form a spore. Salmonella is a pathogenic microorganism that infects humans as well as humans, including pigs, cows, and chickens, causing disease, causing food poisoning in humans, and causing various forms of salmonellosis in animals. Salmonella enterica is classified according to the serological classification into Salmonella Hadar, Salmonella Typhi causing typhoid, Salmonella Typhimurium causing Salmonella typhimurium, Salmonella enteritis disk comprises a species, such as serum (Salmonella Enteritidis), the causative agent of fowl typhoid Salmonella Galina room (Salmonella Gallinarum), the causative agents chubaekri Salmonella flow column (Salmonella Pullorum). Salmonella contains common infectious agents that can cause disease not only in humans but also in livestock, depending on the serotype. Salmonella typhimurium and Salmonella enteritoridis are common infectious diseases and cause food poisoning in humans. In animals such as cattle, chickens and pigs, salmonellosis is caused by acute or chronic enteritis, sepsis, and even pneumonia, Arthritis, and lactic acid. Salmonella is infecting humans with poultry as a medium, and is given direct or indirect contamination opportunities in the entire process of poultry farming, slaughtering and processing. Salmonella is also spread in buildings used by many people, such as hospitals and hospitals. Vertical transmission between mothers and babies and horizontal transmission between babies and babies have been confirmed in obstetrics and gynecology in the UK. Salmonella typhimurium may cause diarrhea-like symptoms as seen in normal salmonellosis, but it may not show such pathological symptoms. Since all patients with diarrhea symptoms are not infected with Salmonella typhi, And the identification of serologic species. Salmonella has been shown to be resistant to amphicillin, tetracycline, and nalidixic acid as a multidrug resistant bacterium, and in France it has been found resistant to amoxicillin and ciprofloxacin. In spite of the danger of Salmonella typhimurium, there is no accurate test on the serotype of Salmonella typhimurium in Korea. Therefore, studies on prevention and treatment of Salmonella typhimurium infection have been actively conducted .

현재 살모넬라 감염증을 예방 및 치료하기 위해 항생제를 주로 사용하고 있으나 살모넬라는 동물에 감염 시 세포내에 침투하여 증식하고 감염하는 경우가 많아 항생제나 약제, 기타 생균제가 침투하여 작용하기는 어려우며, 최근 많은 분야에서 항생제 내성에 대한 문제가 발생되고 있어 이에 대한 원인을 항생제 오남용으로 규명하고 항생제를 충분히 사용하지 못하게 되었다. 이에 따라 산업동물의 배합 사료 내에 성장촉진제로 사용하는 항생제를 금지하기 시작하였고, 유럽연합에서는 2006년부터, 우리나라에서는 2011년 하반기부터 항생제 사용을 규제하기 시작하였다. 또한 항생제가 없는 안전한 먹거리에 대한 소비자의 요구가 증대되고 있어 점차 항생제 사용이 줄어들고 있다. 세균을 제어하는 항생제의 사용이 줄어듦에 따라 세균성 질병의 발생이 증가하고 있어 항생제를 사용하지 않고 세균성 질병을 제어하는 방법에 대한 요구가 늘어나고 있다. 이러한 연구 중 하나가 자연계에 존재하는 세균을 특이적이고 효과적으로 사멸하는 박테리오파지를 이용하는 방법이다. Currently, antibiotics are mainly used for the prevention and treatment of Salmonella infectious diseases. However, Salmonella is infected and penetrates into cells when infected with an animal. Therefore, it is difficult for antibiotics, medicines and other probiotics to penetrate and act, There is a problem about antibiotic resistance, so the reason for this is due to abuse of antibiotics and the use of antibiotics is not enough. As a result, antibiotics used as growth promoters in industrial animal feeds began to be banned. In the EU, in 2006, Korea began regulating the use of antibiotics in the second half of 2011. Consumer demand for safe food without antibiotics is also increasing, and the use of antibiotics is gradually diminishing. As the use of antibiotics to control bacteria decreases, the incidence of bacterial diseases is increasing, and there is a growing demand for methods to control bacterial diseases without the use of antibiotics. One of these studies is the use of bacteriophages that specifically and effectively kill bacteria in the natural world.

박테리오파지(bacteriophage)는 특정 세균을 감염시켜 감염된 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스를 의미한다. 박테리오파지는 박테리아에 감염(infection)한 후 박테리아 세포 내부에서 증식을 하고, 증식 후 자손 박테리오파지들이 박테리아 밖으로 나올 때 숙주인 박테리아의 세포벽을 파괴하는 방식으로 박테리아를 사멸시키는 능력을 갖고 있다. 박테리오파지의 박테리아 감염 방식은 매우 특이성이 높아서 특정 박테리아에 감염할 수 있는 박테리오파지의 종류는 일부로 한정된다. 즉, 특정 박테리오파지는 특정 범주의 박테리아에만 감염할 수 있고 이로 인하여 특정 박테리오파지는 특정 박테리아만을 사멸시키며 다른 박테리아에는 영향을 주지 않는다. 따라서 최근 세균성 질환의 대처 방안으로 박테리오파지(bacteriophage)의 활용이 크게 주목을 받고 있다. 특히 2000년 이후에 항생제 내성균의 증가로 인하여 기존 항생제의 한계성이 나타나고, 기존 항생제의 대체 물질로의 개발 가능성이 부각되면서 다시 박테리오파지가 항-박테리아제로 주목을 받고 있다.Bacteriophages are bacterial-specific viruses that infect certain bacteria and inhibit and inhibit the growth of infected bacteria. Bacteriophages have the ability to kill bacteria by infecting bacteria, multiplying inside bacterial cells, and destroying the cell walls of the host bacteria when the progeny bacteriophages come out of the bacteria after proliferation. The bacteriophage infection method of bacteriophage is highly specific, so the type of bacteriophage that can infect a specific bacterium is limited to some. That is, certain bacteriophages can infect only a specific category of bacteria, which causes certain bacteriophages to kill only certain bacteria and not other bacteria. Recently, the use of bacteriophage has attracted much attention as a countermeasure against bacterial diseases. Especially, since 2000, the limit of existing antibiotics has appeared due to the increase of antibiotic resistant bacteria, and bacteriophage is attracting attention as an anti - bacterial agent again with the possibility of development as a substitute for existing antibiotics.

그러나 현재까지 양돈이나 양계 산업에 막대한 경제적 피해를 일으키고, 사람까지도 감염시킬 수 있는 살모넬라균에 의한 감염증을 예방 또는 치료하기 위한 신규한 박테리오파지에 대한 연구는 여전히 부족한 실정이다. 따라서 살모넬라균에 특이적인 용균활성을 갖는 박테리오파지 및 이의 응용에 대한 기술 개발이 필요하다. However, there is still a lack of research on new bacteriophages to prevent or treat infectious diseases caused by Salmonella which can cause infectious damage to swine and poultry industry and infect people. Therefore, it is necessary to develop a technology for bacteriophage having a lytic activity specific to Salmonella and its application.

이에 본 발명자들은 살모넬라균에 특이적인 용균활성을 갖는 박테리오파지를 분리하고 분리된 박테리오파지의 형태적 및 유전적 특성을 분석하여 상기 박테리오파지가 살모넬라균, 특히 살모넬라 하다를 선택적으로 사멸시킬 수 있음을 확인함으로써 본 발명을 완성하였다.Accordingly, the present inventors have isolated bacteriophage having a lytic activity specific to Salmonella and analyzed the morphological and genetic characteristics of the isolated bacteriophage to confirm that the bacteriophage can selectively kill Salmonella, especially Salmonella, Thereby completing the invention.

본 발명의 목적은 살모넬라균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 SH9을 제공하는 것이다.An object of the present invention is to provide bacteriophage SH9 consisting of the nucleotide sequence shown in SEQ ID NO: 1, which has a killing activity specific to Salmonella.

본 발명의 또 다른 목적은 상기 박테리오파지 SH9을 포함하는, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제 또는 세척제를 제공하는 것이다.It is still another object of the present invention to provide an antibiotic composition, composition for feed addition, feed, disinfectant or detergent comprising the bacteriophage SH9.

본 발명의 또 다른 목적은 상기 박테리오파지 SH9을 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공하는 것이다.Yet another object of the present invention is to provide a method for preventing or treating an infectious disease caused by Salmonella, comprising the step of administering the bacteriophage SH9 to a subject.

상기 목적을 달성하기 위하여, 본 발명은 살모넬라균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 SH9을 제공한다.In order to achieve the above object, the present invention provides bacteriophage SH9 comprising the nucleotide sequence shown in SEQ ID NO: 1, which has a killing activity specific to Salmonella.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 항생용 조성물을 제공한다.Further, the present invention provides an antibiotic composition comprising the bacteriophage SH9.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 사료 첨가용 조성물을 제공한다.The present invention also provides a composition for feed addition comprising the bacteriophage SH9.

또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는, 사료를 제공한다.In addition, the present invention provides a feed comprising the feed composition.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 소독제를 제공한다.The present invention also provides a disinfectant comprising the bacteriophage SH9.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 세척제를 제공한다.The present invention also provides a detergent comprising the bacteriophage SH9.

또한, 본 발명은 상기 박테리오파지 SH9을 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.The present invention also provides a method for preventing or treating an infectious disease caused by Salmonella, comprising the step of administering the bacteriophage SH9 to a subject.

본 발명의 박테리오파지 SH9을 이용한 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법은 기존의 항생제 등의 화학물질에 비해 살모넬라균에 대한 특이성이 매우 높고, 상기 박테리오파지 SH9은 살모넬라균에 감염하여 균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있다. 또한 본 발명의 박테리오파지 SH9는 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 SH9는 살모넬라균, 특히 살모넬라 하다에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.The method for preventing or treating infectious diseases caused by Salmonella using the bacteriophage SH9 of the present invention is highly specific for Salmonella than the chemical substances such as existing antibiotics, and the bacteriophage SH9 is infected with Salmonella, And has the advantage of being strong against physicochemical stimulation. In addition, the bacteriophage SH9 of the present invention is not infected by any host other than bacteria such as human, animal, and plant. Therefore, it is possible to solve problems of antibiotic resistant bacteria caused by misuse of antibiotics, residual antibiotics in food, have. Accordingly, the bacteriophage SH9 of the present invention can be variously used in the field of preventing or treating infectious diseases caused by Salmonella, especially Salmonella, antibiotic composition, composition for feed addition, feed, disinfectant, or detergent.

도 1은 박테리오파지 SH9의 살모넬라 하다에 대한 용균반 형성 활성을 확인한 결과를 나타낸 도이다.
도 2는 박테리오파지 SH9의 유전자 맵을 나타낸 도이다.
도 3은 전자 현미경을 통해 박테리오파지 SH9의 형태학적 특성을 확인한 결과를 나타낸 도이다.
도 4는 박테리오파지 SH9의 단백질 패턴을 SDS-PAGE를 통해 확인한 결과를 나타낸 도이다.
도 5는 박테리오파지 SH9의 pH 안정성을 확인한 결과를 나타낸 도이다.
도 6은 박테리오파지 SH9의 열 안정성을 확인한 결과를 나타낸 도이다.Brief Description of the Drawings Fig. 1 is a graph showing the result of confirming the dissolution activity of Salmonella spp. Of bacteriophage SH9.
2 is a diagram showing a gene map of bacteriophage SH9.
FIG. 3 is a diagram showing the result of confirming morphological characteristics of bacteriophage SH9 through an electron microscope.
4 is a diagram showing the result of SDS-PAGE of the protein pattern of bacteriophage SH9.
5 is a graph showing the result of confirming the pH stability of bacteriophage SH9.
6 is a diagram showing the result of checking the thermal stability of bacteriophage SH9.

이하, 본 발명에 대해 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명은 살모넬라균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어진 박테리오파지 SH9을 제공한다.The present invention provides a bacteriophage SH9 consisting of the nucleotide sequence shown in SEQ ID NO: 1, which has a killing activity specific to Salmonella.

본 발명에서, 용어 박테리오파지(bacteriophage)는 특정 세균에 감염하여 당해 세균의 성장을 억제하고 저해하는 세균 특이적 바이러스로, 단일 혹은 이중 사슬의 DNA 또는 RNA를 유전 물질로 포함하는 바이러스를 의미한다.In the present invention, the term bacteriophage refers to a bacterium-specific virus that infects a specific bacterium and inhibits and inhibits the growth of the bacterium, and includes a single or double-stranded DNA or RNA as a genetic material.

본 발명에서, 상기 살모넬라균은 살모넬라 하다(Salmonella Hadar) 또는 살모넬라 티피뮤리움(Salmonella Typhimurium)일 수 있으며, 바람직하게는 살모넬라 하다이다.In the present invention, the Salmonella may be Salmonella Hadar or Salmonella Typhimurium, preferably Salmonella.

본 발명에서, 상기 살모넬라 하다는 다제내성(multidrug resistance) 살모넬라 하다일 수 있으며, 예를 들어, 앰피실린, 테트라사이클린, 날리딕스산, 아목시실린 또는 시프로플록사신 등의 항생제에 대한 내성을 가진 살모넬라 하다를 포함하나, 이에 제한되는 것은 아니다. 본 발명에서, 상기 살모넬라 하다는 다제내성(multidrug resistance) 살모넬라 하다일 수 있으며, 예를 들어, 앰피실린, 테트라사이클린, 날리딕스산, 아목시실린 또는 시프로플록사신 등의 항생제에 대한 내성을 가진 살모넬라 하다를 포함하나, 이에 제한되는 것은 아니다.In the present invention, the Salmonella may be a multidrug resistance salmonella and includes, for example, Salmonella strains resistant to antibiotics such as ampicillin, tetracycline, nalidixic acid, amoxicillin or ciprofloxacin, But is not limited thereto. In the present invention, the Salmonella may be a multidrug resistance salmonella and includes, for example, Salmonella strains resistant to antibiotics such as ampicillin, tetracycline, nalidixic acid, amoxicillin or ciprofloxacin, But is not limited thereto.

본 발명의 박테리오파지 SH9은 살모넬라균, 특히 살모넬라 하다에 대해 용균활성이 탁월하다. 상기 박테리오파지 SH9은 200-240 nm의 크기로, 정이십면체의 머리를 가지며 수축성의 꼬리를 가지는 형태인 마이오비리대(Myoviridae)에 속하며, 단백질 패턴 분석 결과 64 kDa, 58 kDa, 36 kDa, 35 kDa, 34 kDa 그리고 30 kDa 크기의 단백질을 포함함을 확인하였다.The bacteriophage SH9 of the present invention has excellent lytic activity against Salmonella, especially Salmonella. The bacteriophage SH9 belongs to Myoviridae, which is 200-240 nm in size and has a truncated conformational tail and has a contractile tail. As a result of protein pattern analysis, 64 kDa, 58 kDa, 36 kDa and 35 kDa , 34 kDa and 30 kDa, respectively.

또한 상기 박테리오파지 SH9은 열 및 pH에 대한 안정성이 우수한 특성을 가지고 있다.In addition, the bacteriophage SH9 has excellent stability against heat and pH.

박테리오파지 SH9은 서열번호 1로 표시되는 염기서열을 전체 유전자의 전체 또는 일부로서 포함할 수 있다. 또한, 본 발명의 박테리오파지 SH9은 서열번호 1로 표시되는 염기서열, 및 상기 염기서열의 기능적 동등물로 이루어질 수 있다. 상기 기능적 동등물이란 염기서열의 변형, 치환의 결과, 상기 서열번호 1로 표시되는 염기서열과 적어도 70% 이상, 바람직하게는 80% 이상, 더욱 바람직하게는 90% 이상, 더더욱 바람직하게는 95% 이상의 서열 상동성을 갖는 것으로, 서열번호 1로 표시되는 염기서열과 실질적으로 동질의 생리활성을 나타내는 서열을 의미한다.Bacteriophage SH9 may contain the nucleotide sequence shown in SEQ ID NO: 1 as whole or part of the entire gene. In addition, the bacteriophage SH9 of the present invention may comprise the nucleotide sequence shown in SEQ ID NO: 1 and the functional equivalent of the nucleotide sequence. The functional equivalent means at least 70% or more, preferably 80% or more, more preferably 90% or more, still more preferably 95% or more of the nucleotide sequence shown in SEQ ID NO: 1 as a result of modification or substitution of the nucleotide sequence. Or more and having substantially the same physiological activity as the nucleotide sequence shown in SEQ ID NO: 1.

상기와 같은 살모넬라균 특이적 용균활성, 내산성, 내염기성 및 내열성은 본 발명의 박테리오파지 SH9을 살모넬라균에 의한 감염성 질병의 예방 및 치료용 조성물, 및 상기 박테리오파지 SH9을 유효성분으로 포함하는 다양한 제품에 적용함에 있어, 다양한 온도 및 pH 범위의 적용이 가능하게 한다.The salmonella-specific lytic activity, acid resistance, basic basicity and heat resistance as described above can be applied to compositions for preventing and treating infectious diseases caused by Salmonella bacteria and various products containing the bacteriophage SH9 as an active ingredient This makes it possible to apply various temperature and pH ranges.

본 발명의 박테리오파지 SH9은 도축장 및 양계장 부근의 하수로부터 시료를 채취하여 분리한 박테리오파지로서, 본 발명자는 박테리오파지 SH9로 명명하고 2016년 12월 02일에 한국미생물보존센터 (Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하여, 수탁번호 KFCC11708P를 부여받았다.The bacteriophage SH9 of the present invention is a bacteriophage obtained by collecting a sample from sewage near the slaughterhouse and poultry house. The inventor named the bacteriophage SH9 as bacteriophage SH9 and the Korean Culture Center of Microorganisms (Seoul, Korea) on December 02, Korea), and received the deposit number KFCC11708P.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for preventing or treating infectious diseases caused by Salmonella, which comprises the bacteriophage SH9.

본 발명에서, 상기 살모넬라균은 살모넬라 하다(Salmonella Hadar) 또는 살모넬라 티피뮤리움(Salmonella Typhimurium)일 수 있으며, 바람직하게는 살모넬라 하다이다.In the present invention, the Salmonella may be Salmonella Hadar or Salmonella Typhimurium, preferably Salmonella.

본 발명의 박테리오파지 SH9은 살모넬라균에 특이적인 사멸능을 가지므로, 살모넬라균에 의한 감염성 질병의 예방 또는 치료용 약학적 조성물에 사용될 수 있다. 본 발명의 박테리오파지 SH9은 살모넬라균 중에서도 살모넬라 하다에 더욱 특이적이므로, 본 발명은 살모넬라 하다 감염에 의한 감염성 질병의 예방 또는 치료에 이용될 수 있으나, 이에 제한되는 것은 아니다.The bacteriophage SH9 of the present invention has an ability to kill salmonella-specific and thus can be used in a pharmaceutical composition for preventing or treating infectious diseases caused by Salmonella. Since the bacteriophage SH9 of the present invention is more specific to Salmonella among Salmonella, the present invention can be used for prevention or treatment of infectious diseases caused by Salmonella spp. Infection, but is not limited thereto.

본 발명에서, 살모넬라균에 의한 감염성 질병은 유행성 또는 급성으로 발병하는 전염성 질환을 통칭하는 개념으로, 살모넬라증 외에 식중독, 장염, 폐렴 및 패혈증 등을 포함하나, 이에 제한되는 것은 아니다.In the present invention, an infectious disease caused by Salmonella is a concept collectively referred to as an infectious disease that occurs epidemically or acutely. It includes, but is not limited to, food poisoning, enteritis, pneumonia and sepsis as well as salmonellosis.

상기 살모넬라증은 살모넬라균 감염에 의해 발열, 두통, 설사, 구토 등을 수반하는 증상을 총칭하는 것으로, 살모넬라증은 장티푸스와 같은 증세를 나타내는 패혈증형과 식중독 증세를 나타내는 급성위장염형으로 대별되며, 장염, 식중독, 급성 균혈증 등이 포함된다.Salmonellosis is a general term referring to symptoms accompanied by fever, headache, diarrhea, and vomiting due to Salmonella infection. Salmonellosis is classified into septicemia, which is similar to typhoid fever, and acute gastroenteritis, which is a food poisoning symptom. , Acute bacteremia, and the like.

본 발명의 약학적 조성물은 1 × 103 내지 1 × 1010 PFU/mL의 박테리오파지를 포함하며, 바람직하게는 1 × 106 내지 1 × 109 PFU/mL의 박테리오파지를 포함한다. 본 발명에 사용된 용어, PFU(plaque forming unit)는 박테리오파지가 플라크를 형성하는 것을 수치화한 단위이다.The pharmaceutical composition of the present invention contains bacteriophage of 1 × 10 3 to 1 × 10 10 PFU / mL, preferably 1 × 10 6 to 1 × 10 9 PFU / mL of bacteriophage. The term " PFU (plaque forming unit) " used in the present invention is a unit in which bacteriophages form a plaque.

본 발명의 용어 예방이란 조성물의 투여로 질병을 억제시키거나 발병을 지연시키는 모든 행위를 의미한다.The term " prophylactic " of the present invention means all actions that inhibit disease or delay the onset of the disease by administration of the composition.

본 발명의 용어 치료란 조성물의 투여로 상기 질병의 증세가 호전되거나 상기 질병의 억제 또는 경감 및 이롭게 변경되는 모든 행위를 의미한다.The term " treatment " of the present invention refers to any action that improves or alleviates or alleviates the symptoms of the disease upon administration of the composition.

본 발명의 약학적 조성물은 약학적으로 허용 가능한 담체를 추가로 포함할 수 있다.The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier.

본 발명에서 용어, 약학적으로 허용 가능한 담체란 생물체를 자극하지 않고 투여 화합물의 생물학적 활성 및 특성을 저해하지 않는 담체 또는 희석제를 의미한다. 액상 용액으로 제제화되는 조성물에 있어서 허용되는 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로오스 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.The term pharmaceutically acceptable carrier, as used herein, means a carrier or diluent that does not irritate the organism and does not interfere with the biological activity and properties of the administered compound. Examples of the pharmaceutical carrier which is acceptable for the composition to be formulated into a liquid solution include sterilized and sterile water, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

본 발명의 약학적 조성물은 질환 부위에의 도포 또는 분무하는 방법으로 이용할 수 있으며, 그 밖에 비강 분무, 경구 투여 또는 비경구 투여를 통해 투여할 수도 있으며, 비경구 투여의 경우 정맥 내 투여, 복강 내 투여, 근육 내 투여, 피하 투여 또는 국부 투여를 이용하여 투여할 수도 있다.The pharmaceutical composition of the present invention can be used as a method of applying or spraying on a diseased site, or may be administered through nasal spray, oral administration or parenteral administration. In the case of parenteral administration, intravenous administration, intraperitoneal administration Administration, intramuscular, subcutaneous or local administration.

상기 비강 분무의 경우 일정량의 박테리오파지 SH9을 물에 희석하여 분무기나 분무 시스템을 통해 비강으로 흡수 되도록 할 수 있다. 상기 비강 분무를 위한 비강 분무제 또는 호흡기용 제형으로는 에어로졸제 등을 들 수 있다.In the case of the nasal spray, a certain amount of bacteriophage SH9 can be diluted in water and absorbed into the nasal cavity through a sprayer or spray system. Examples of the nasal spray agent for the nasal spray or the aerosol agent for the respiratory system are mentioned.

상기 비강 분무제 또는 호흡기용 제형은, 수분산된 농축물 또는 습윤 분말을 분산시키기 위한 추진제 및/또는 기타 첨가제를 추가로 포함할 수 있다.The nasal spray or respiratory formulation may further comprise a propellant and / or other additives for dispersing the water-dispersed concentrate or wet powder.

본 발명의 약학적 조성물의 적합한 도포, 분무 및 투여량은 제제화 방법, 투여 방식, 대상이 되는 동물 및 환자의 연령, 체중, 성, 질병 증상의 정도, 음식, 투여시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사나 수의사는 목적하는 치료에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다.The appropriate application, spray and dose of the pharmaceutical composition of the present invention may vary depending on factors such as formulation method, administration method, age, body weight, sex, disease symptom level, food, administration time, administration route, Response sensitivities, and the ordinarily skilled physician or veterinarian can readily determine and prescribe dosages effective for the desired treatment.

본 발명의 약학적 조성물을 유효성분으로 포함하는 경구 투여용 제형으로는, 예를 들어 정제, 트로키제, 로렌지, 수용성 또는 유성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제로 제제화할 수 있다. 정제 및 캡슐 등의 제형으로 제제화하기 위해, 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀룰로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕괴제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유를 포함할 수 있으며, 캡슐 제형의 경우 상기 언급한 물질 외에도 지방유와 같은 액체 담체를 더 함유할 수 있다.Examples of formulations for oral administration comprising the pharmaceutical composition of the present invention as an active ingredient include tablets, troches, lozenges, aqueous or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs It can be formulated into a zero. A binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin, an excipient such as dicalcium phosphate, a disintegrating agent such as corn starch or sweet potato starch, a disintegrant such as magnesium stearate , Calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned materials, a liquid carrier such as a fatty oil may be further contained.

본 발명의 약학적 조성물을 유효성분으로 포함하는 비경구 투여용 제형으로는, 피하주사, 정맥주사 또는 근육 내 주사 등의 주사용 형태, 좌제 주입방식 또는 호흡기를 통하여 흡입이 가능하도록 하는 에어로졸제 등 스프레이용으로 제제화할 수 있다. 주사용 제형으로 제제화하기 위해서는 본 발명의 조성물을 안정제 또는 완충제와 함께 물에서 혼합하여 용액 또는 현탁액으로 제조하고, 이를 앰플 또는 바이알의 단위 투여용으로 제제화할 수 있다. 에어로졸제 등의 스프레이용으로 제형화하는 경우, 수분산된 농축물 또는 습윤 분말이 분산되도록 추진제 등이 첨가제와 함께 배합될 수 있다.Examples of the formulations for parenteral administration containing the pharmaceutical composition of the present invention as an active ingredient include injection forms such as subcutaneous injection, intravenous injection or intramuscular injection, aerosol formulations such as a suppository injection method or a respiratory apparatus It can be formulated for spraying. For formulation into injectable formulations, the compositions of the present invention may be formulated as solutions or suspensions in water with stabilizers or buffers in water, and formulated for unitary administration of ampoules or vials. When formulated for spraying, such as an aerosol formulation, a propellant or the like may be formulated with the additive such that the water-dispersed concentrate or wet powder is dispersed.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 항생용 조성물을 제공한다. Further, the present invention provides an antibiotic composition comprising the bacteriophage SH9.

본 발명에서, 용어 항생용 조성물은 약제 형태로 동물에게 제공되어 균을 사멸시킬 수 있는 제제를 의미하며, 방부제, 살균제, 항생제 및 항균제를 총칭하는 것이다. In the present invention, the term antibiotic composition refers to a preparation which can be provided to an animal in the form of a drug to kill bacteria, and is collectively referred to as an antiseptic, a bactericide, an antibiotic and an antibacterial agent.

본 발명의 박테리오파지 SH9은 기존 항생제에 비하여 살모넬라균에 대한 특이성이 매우 높으므로, 유익균은 죽이지 않고, 특정 병원균만 사멸시킬 수 있고, 약물 내성 내지 저항성을 유도하지 않아, 기존의 항생물질에 비하여 제품수명(life cycling)이 긴 신규 항생제로서 이용될 수 있다.The bacteriophage SH9 of the present invention has a higher specificity to Salmonella than the existing antibiotics. Therefore, the bacteriophage SH9 of the present invention can kill only specific pathogens and does not induce drug resistance or resistance, (life cycling) can be used as a new antibiotic.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 사료 첨가용 조성물을 제공한다.The present invention also provides a composition for feed addition comprising the bacteriophage SH9.

축산, 수산업에서 사용되는 사료 첨가용 항생제는 질병의 예방 목적으로 사용되고 있는데, 예방 목적의 항생제 투여는 내성균 발생 가능성을 높이고 가축에 잔류하는 항생제가 사람에게 전달될 수 있어서 문제이다. 항생제가 육류를 통해 인체에 흡수되면 항생제 내성을 유발해 질병의 확산을 부를 수도 있다. 또한, 사료에 섞어 먹이는 항생제의 종류가 많고 이는 다제 내성균 발생 확률이 높아지는 문제점이 있기 때문에 좀 더 자연 친화적이면서도 기존의 항생제의 사용에서 발생한 문제를 해결할 새로운 사료 첨가용 항생물질로서 본 발명의 상기 박테리오파지 SH9을 이용할 수 있다.Antibiotics for feed additive used in animal husbandry and fisheries are used for the prevention of diseases. The administration of antibiotics for prevention is problematic because it increases the possibility of producing resistant bacteria and antibiotics remaining in the livestock can be delivered to humans. If antibiotics are absorbed into the body through meat, they may cause antibiotic resistance and spread the disease. In addition, since there are many types of antibiotics to be mixed with feed, there is a problem that the probability of occurrence of multidrug-resistant bacteria increases. Therefore, as a new antibiotic substance for food addition which is more environmentally friendly and solves the problem caused by the use of existing antibiotics, the bacteriophage SH9 Can be used.

또한, 본 발명은 상기 사료 첨가용 조성물을 포함하는 사료를 제공할 수 있으며, 본 발명의 사료는 박테리오파지를 사료 첨가제 형태로 따로 제조하여 사료에 혼합시키거나, 사료 제조 시 직접 첨가시켜 제조할 수 있다. 본 발명의 사료 내 박테리오파지는 액상 또는 건조상태일 수 있으며, 바람직하게는 건조된 분말형태이다. 건조방법은 통풍건조, 자연건조, 분무건조 및 동결건조가 가능하지만, 이에 제한되는 것은 아니다. 본 발명의 박테리오파지는 분말형태로 사료 중량의 0.05 내지 10 중량%, 바람직하게는 0.1 내지 2 중량%의 성분비로 혼합될 수 있다. 또한, 상기 사료는 본 발명의 박테리오파지 외에 사료의 보존성을 높일 수 있는 통상의 첨가제들을 추가로 포함할 수 있다.In addition, the present invention can provide a feed comprising the feed composition, and the feed of the present invention can be prepared by separately preparing bacteriophage as a feed additive and mixing the feed into the feed, . The bacteriophage in the feed of the present invention may be in a liquid or dry state, preferably in the form of a dried powder. Drying methods include, but are not limited to, air drying, natural drying, spray drying and freeze drying. The bacteriophage of the present invention may be mixed in powder form in a composition ratio of 0.05 to 10% by weight, preferably 0.1 to 2% by weight of the feed weight. In addition to the bacteriophages of the present invention, the feed may further include conventional additives that can enhance the preservability of the feed.

본 발명의 사료 첨가용 조성물에는 비병원성의 다른 미생물이 추가로 첨가될 수 있다. 첨가될 수 있는 미생물로는 단백질 분해 효소, 지질 분해 효소 및 당 전환 효소를 생산할 수 있는 바실러스 서브틸리스(Bacillus subtilis)와 같은 고초균, 소의 위와 같은 혐기적 조건에서 생리적 활성 및 유기물 분해능이 있는 락토바실러스 균주(Lactobacillus sp.), 가축의 체중을 증가시키며 우유의 산유량을 늘리고 사료의 소화 흡수율을 높이는 효과를 보여주는 아스퍼질러스 오리자에(Aspergillus oryzae)와 같은 사상균 및 사카로미세스 세레비지에(Saccharomyces cerevisiae)와 같은 효모로 구성된 군으로부터 선택될 수 있다.In the composition for feed addition of the present invention, microorganisms other than non-pathogenic microorganisms may be further added. Examples of microorganisms that can be added include lactobacilli such as Bacillus subtilis capable of producing proteolytic enzymes, lipolytic enzymes and glycosyltransferases, lactobacilli having physiological activity and organic resolution at anaerobic conditions such as bovine, ( Lactobacillus sp.), Filamentous fungi such as Aspergillus oryzae , which increase the body weight of livestock and increase the milk yield of milk and increase digestion rate of feed, and Saccharomyces cerevisiae ). ≪ / RTI >

본 발명의 박테리오파지 SH9을 포함하는 사료에는 식물성으로는 곡물류, 근과류, 식품가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류, 곡물부산물류 등이 있으며, 동물성으로는 단백질류, 무기물류, 유지류, 광물성류, 단세포 단백질, 동물성 플랑크톤류, 남은 음식물 등이 있으며 이에 제한되는 것은 아니다.Examples of the feedstuffs containing bacteriophage SH9 of the present invention include cereals, muscle roots, food processing, logistics, algae, fibers, pharmaceutical products, fats and oils, pastes, But are not limited to, proteins, mineralogy, oils, mineralogy, unicellular proteins, zooplankton, and remnants.

본 발명의 사료 첨가용 조성물에는 품질 저하를 방지하기 위하여 첨가하는 결착제, 유화제, 보존제 등이 포함될 수 있고, 효용 증대를 위하여 사료에 첨가하는 아미노산제, 비타민제, 효소제, 생균제, 향미제, 비단백태질소화합물, 규산염제, 완충제, 착색제, 추출제, 올리고당 등이 포함될 수 있으며, 그 외에도 사료 혼합제 등을 추가로 포함할 수 있다.The composition for feed addition of the present invention may contain a binder, an emulsifying agent, a preservative, etc. added to prevent deterioration of quality. An amino acid, a vitamin, an enzyme, a probiotic, a flavoring agent, A nitrogen compound, a silicate, a buffer, a coloring agent, an extracting agent, an oligosaccharide, etc., and may further include a feed mixture.

또한, 본 발명은 박테리오파지 SH9을 포함하는, 음용수 첨가제를 제공한다.The present invention also provides a drinking water additive comprising bacteriophage SH9.

본 발명의 음용수 첨가제는 상기 박테리오파지 SH9 또는 이를 포함하는 조성물을 음용수 첨가제 형태로 따로 제조하여 사료 또는 음용수에 혼합하는 방식으로 사용되거나, 음용수 제조 시 직접 첨가하는 방식으로 사용할 수 있다. 상기와 같이 음용수에 혼합하여 공급함으로써 지속적으로 살모넬라균의 숫자를 감소시킬 수 있는 효과가 있다.The additive for drinking water of the present invention can be used either by preparing the bacteriophage SH9 or a composition containing the bacteriophage SH9 separately in the form of additive for drinking water, mixing it with feed or drinking water, or by directly adding it in the production of drinking water. As a result, the number of salmonella bacteria can be continuously reduced by mixing and supplying the drinking water.

본 발명에서 음용수는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 음용수를 사용할 수 있다.In the present invention, the drinking water is not particularly limited, and drinking water commonly used in the art can be used.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 소독제를 제공한다.The present invention also provides a disinfectant comprising the bacteriophage SH9.

살모넬라균, 예컨대 살모넬라 하다 또는 살모넬라 티피뮤리움에 특이적 사멸능을 갖는 본 발명의 박테리오파지 SH9을 포함하는 소독제는 병원감염을 막기 위한 병원 및 보건용의 소독제로 유용하게 사용될 수 있고 일반 생활 소독제, 식품 및 조리 장소 및 설비의 소독제, 양계장, 축사 등의 건물, 축체, 음수, 깔짚, 난좌, 운반차량, 식기 등의 각종 생육 용품의 소독 등에 사용될 수 있다.The disinfectant containing the bacteriophage SH9 of the present invention having a specific killing power against Salmonella such as Salmonella typhimurium or Salmonella typhimurium can be usefully used as a disinfectant for hospital and health to prevent hospital infection, And disinfection of various kinds of growing equipment such as disinfectants for poultry houses and facilities, buildings such as poultry houses, housing facilities, shafts, water, litter, stairs, transportation vehicles, tableware and the like.

또한, 본 발명은 상기 박테리오파지 SH9을 포함하는, 세척제를 제공한다.The present invention also provides a detergent comprising the bacteriophage SH9.

본 발명의 박테리오파지 SH9은 살모넬라균, 예컨대 살모넬라 하다 또는 살모넬라 티피뮤리움에 특이적 사멸능을 가지므로, 상기 살모넬라균에 노출되었거나 노출될 가능성이 있는 가축의 피부 표면 또는 신체 각 부위 등을 세척하는 용도로도 사용될 수 있다.The bacteriophage SH9 of the present invention has a specific killing power against Salmonella, such as Salmonella typhimurium or Salmonella typhimurium, so that it can be used for washing the skin surface or body parts of a livestock that is exposed or exposed to the Salmonella Can also be used.

또한, 본 발명은 상기 박테리오파지 SH9을 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법을 제공한다.The present invention also provides a method for preventing or treating an infectious disease caused by Salmonella, comprising the step of administering the bacteriophage SH9 to a subject.

본 발명의 방법은 살모넬라균에 의한 감염성 질병, 예컨대 유행성 또는 급성으로 발병하는 전염성 질환으로, 살모넬라증 외에 식중독, 장염, 폐렴 또는 패혈증이 발생한 개체 또는 발생 위험이 있는 개체에 박테리오파지 SH9을 투여하는 단계를 포함한다. The method of the present invention includes the step of administering bacteriophage SH9 to an infectious disease caused by Salmonella, for example, an epidemic or acute infectious disease, a food poisoning, enteritis, pneumonia or septicemia other than salmonellosis, do.

본 발명에서, 용어 "개체"는 살모넬라균에 의한 감염성 질병이 발병될 수 있는 모든 개체를 의미하는 것으로 인간을 포함한 포유동물, 가축류 또는 가금류를 포함하나, 이에 제한되는 것은 아니다. In the present invention, the term "individual " means any individual to which an infectious disease caused by Salmonella can be developed, including, but not limited to, mammals including humans, livestock or poultry.

상기 용어 "가축류"는 인간에 의하여 순화, 개량되어 사람과 함께 공동생활을 하는 유용한 동물을 지칭하는 개념으로, 예를 들어, 돼지, 소, 닭, 말, 오리 또는 개 등을 포함하나, 이에 제한되는 것은 아니다.The term " livestock animal "is intended to refer to useful animals that are refined and improved by humans to co-live with humans, including, for example, pigs, cows, chickens, horses, ducks or dogs, It is not.

상기 용어 "가금류"는, 가축 중 조류에 속하는 동물을 총칭하여 이르는 개념으로, 예를 들어, 닭, 오리, 꿩, 메추리, 타조, 거위 또는 칠면조 등을 포함하나, 이에 제한되는 것은 아니다.The term "poultry" includes, but is not limited to, chicken, duck, pheasant, queen, ostrich, goose or turkey.

본 발명의 방법에서 상기 박테리오파지 SH9 또는 조성물은 약학적 제제의 형태로 동물에게 투여되거나, 가축의 사료 또는 음용수에 혼합하여 이를 섭식시키는 방법을 통해 투여될 수 있다.In the method of the present invention, the bacteriophage SH9 or the composition may be administered to an animal in the form of a pharmaceutical preparation, or mixed with feed or drinking water of a livestock and fed.

본 발명의 방법에서 상기 박테리오파지 SH9 또는 조성물의 투여 경로는 목적 조직에 도달할 수 있는 한 경구 또는 비경구의 다양한 경로를 통하여 투여될 수 있으며, 구체적으로, 구강, 직장, 국소, 정맥 내, 복강 내, 근육 내, 동맥 내, 경피, 비측 내, 흡입 등을 통해 통상적인 방식으로 투여될 수 있다.In the method of the present invention, the administration route of the bacteriophage SH9 or composition can be administered through various routes of oral or parenteral administration as long as it can reach the target tissues. Specifically, the oral route, rectal, topical, intravenous, Intravenous, intramuscular, intraarterial, transdermal, intranasal, inhalation, and the like.

본 발명의 방법에 투여되는 상기 박테리오파지 SH9의 적합한 총 1일 사용량은 올바른 의학적 판단범위 내에서 처치의에 의해 결정될 수 있다는 것은 당업자에게 자명한 일이다. 특정 개체에 대한 구체적인 치료적 유효량은 달성하고자 하는 반응의 종류와 정도, 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여시간, 투여 경로 및 조성물의 분비율, 치료기간, 구체적 조성물과 함께 사용되거나 동시 사용되는 약물을 비롯한 다양한 인자와 의약 분야에 잘 알려진 유사 인자에 따라 다르게 적용하는 것이 바람직하다.It will be apparent to those skilled in the art that the appropriate total daily usage of the bacteriophage SH9 administered in the methods of the present invention can be determined by treatment within the scope of sound medical judgment. The specific therapeutically effective amount for a particular individual will depend upon the type and extent of the response to be achieved, the age, weight, general health, sex and diet of the patient, the time of administration, the route of administration and the fraction of the route of administration, It is preferable to apply differently depending on various factors including drugs used or co-used and similar factors well known in the medical field.

본 명세서에서 달리 정의되지 않은 용어들은 본 발명이 속하는 기술분야에서 통상적으로 사용되는 의미를 갖는 것이다.Terms not otherwise defined herein have meanings as commonly used in the art to which the present invention belongs.

이하, 본 발명을 실시예, 제제예에 의해 상세히 설명한다. 단, 하기 실시예, 제제예는 본 발명을 예시하는 것일 뿐 본 발명의 내용이 하기 실시예, 제제예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail with reference to Examples and Preparation Examples. However, the following examples and preparative examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples and preparative examples.

실시예 1: 신규한 박테리오파지의 분리 및 배양Example 1: Isolation and culture of novel bacteriophages

1.1 박테리오파지의 스크리닝 및 단일 박테리오파지의 분리1.1 Screening of bacteriophages and isolation of single bacteriophages

살모넬라 하다에 특이적인 박테리오파지를 스크리닝 및 분리하기 위해서 용균반 분석을 수행하였다. 시료는 박테리오파지가 존재할 것으로 예상되는 도축장 및 양계장 부근의 하수로부터 시료를 채취하였으며, 박테리오파지 분리에 사용된 살모넬라 하다는 (주)옵티팜에서 채취한 야외 시료로부터 분리 동정된 것을 사용하였다.Sputum analysis was performed to screen and isolate bacteriophages specific to Salmonella typhimurium. Samples were taken from the slaughterhouse and the sewage near the poultry farm where bacteriophages were expected to be present. Salmonella harbor used for bacteriophage isolation was isolated from outdoor samples taken from Opti Farm.

구체적으로, 채취한 시료를 균질화 한 후 살모넬라 하다와 함께 35℃에서 24시간 동안 100 rpm으로 진탕배양 하였다. 상기 배양액을 3,000 rpm으로 25분간 원심분리한 후, 상등액을 공극(pore) 크기가 0.45 μm인 필터를 이용하여 여과하여 시료 여과액을 얻었다. Specifically, the collected samples were homogenized and cultured with Salmonella spp. At 35 DEG C for 24 hours with shaking at 100 rpm. The culture was centrifuged at 3,000 rpm for 25 minutes, and the supernatant was filtered using a filter having a pore size of 0.45 μm to obtain a sample filtrate.

전배양된 살모넬라 하다 150 μL를 취해서 멸균된 튜브에 넣고 3 mL의 LB 탑 아가(top agar) (LB 배지(트립톤 10 g/L, 효모 추출물(yeast extract) 5 g/L, NaCl 10 g/L) + 0.6% 아가)를 첨가하여 잘 혼합한 후 미리 만들어 둔 2% LB 아가 플레이트에 중층하였다. 이를 실온에서 30분간 방치한 후 상기 시료 여과액을 각각 10 μL씩 점적하고, 다시 실온에서 30분간 방치한 후 35℃에서 24시간 동안 배양하여 박테리오파지의 유무를 확인하였다.150 μL of the pre-cultured Salmonella strains were placed in sterilized tubes and 3 mL of LB top agar (LB medium (10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L) + 0.6% agar) was added and mixed well. Then, the mixture was layered on a 2% LB agar plate previously prepared. After incubation at room temperature for 30 minutes, 10 μL of the sample filtrate was added dropwise, and the mixture was allowed to stand at room temperature for 30 minutes and then incubated at 35 ° C. for 24 hours to confirm presence or absence of bacteriophages.

박테리오파지의 존재를 확인한 시료로부터 박테리오파지를 순수 분리하기 위해서 소프트 아가 오버레이(soft agar overlay)법을 이용하였다. 박테리오파지가 존재하는 것으로 확인된 시료를 식염수로 적절히 희석하여 각 희석단의 시료 희석액 100 μL를 취하고, 대상 세균(살모넬라 하다) 배양액 150 μL에 혼합한 후 0.6% LB 탑 아가 3 mL을 첨가하여 잘 혼합한 뒤, 미리 만들어 둔 2% LB 아가 플레이트에 중층하여 방치한 후 35℃에서 15시간 배양하였다. 배양한 각 플레이트에서 형성된 용균반을 확인한 후, 이를 선택하여 400 μL의 SM완충액 (NaCl 5.8 g/L; MgSO47H2O 2 g/L; 1M Tris-HCl (pH 7.5) 50 mL)에 현탁하고, 실온에서 4시간 동안 방치하였다. 상기 박테리오파지액 200 μL를 0.6% LB 탑 아가와 세균 배양액 300 μL와 혼합한 뒤 150 mm 지름의 LB 플레이트에 분주하여 중층한 후 완전히 용균이 일어날 때까지 35℃에서 배양하였다. 배양이 종료된 후, LB 플레이트에 15 mL의 SM 완충액을 가하고 실온에서 4시간 동안 천천히 교반하면서 박테리오파지 용액을 회수한 후 3,000 rpm으로 20분간 원심분리하여 상등액만 취하고 이를 0.45 μm 필터로 여과하여 최종 박테리오파지 용액을 회수하였다. 이렇게 얻어진 최종 박테리오파지의 용균반을 확인한 결과를 도 1에 나타내었다.A soft agar overlay method was used to isolate the bacteriophage from the sample which confirmed the existence of the bacteriophage. The sample, which has been confirmed to contain bacteriophage, is diluted appropriately with saline, 100 μL of sample dilution at each dilution stage is taken, mixed with 150 μL of the bacterial (salmonella) culture, and 3 mL of 0.6% LB topa After incubation, the cells were allowed to stand in a 2% LB agar plate that had been previously prepared, followed by incubation at 35 ° C for 15 hours. After incubation, the supernatant was added to 400 μL of SM buffer (NaCl 5.8 g / L; MgSO 4 7H 2 O 2 g / L; 1M Tris-HCl (pH 7.5) And allowed to stand at room temperature for 4 hours. 200 μL of the bacteriophage solution was mixed with 300 μL of 0.6% LB top agar and bacterial culture, and then the mixture was divided into 150-mm-diameter LB plates and cultured at 35 ° C. until complete lysis. After incubation, 15 mL of SM buffer was added to the LB plate, and the bacteriophage solution was recovered while slowly stirring at room temperature for 4 hours. The supernatant was centrifuged at 3,000 rpm for 20 minutes. The supernatant was collected by filtration through a 0.45 μm filter, The solution was recovered. FIG. 1 shows the result of checking the thus obtained final bacteria bacterium.

도 1에 나타낸 바와 같이, 1-2 mm의 크기를 가지는 용균반을 확인함으로써, 상기 박테리오파지가 살모넬라 하다에 특이적인 용균능을 갖는 박테리오파지임을 확인하였다.As shown in Fig. 1, the bacteriophage was found to be a bacteriophage having a solubility specific to Salmonella, by confirming a frying pan having a size of 1-2 mm.

1.2 박테리오파지의 대량 배양 및 정제1.2 Bulk culture and purification of bacteriophages

상기 실시예 1.1에서 확인한 살모넬라 하다 특이적 박테리오파지를 대량 배양하기 위하여, 1.1에서 회수한 최종 박테리오파지 배양액을 1.0x109 PFU/mL로 희석한 후 전배양된 살모넬라 하다 1.0x1010 CFU/mL과 혼합하고 MOI(Multiplicity of infection)를 0.1이 되도록 하여 35℃에서 100 rpm으로 24시간 동안 배양하였다. 배양이 종료된 후 배양액 전량을 6,000 rpm으로 25분간 원심분리하여 상등액을 수득하였고, 이를 0.22 μm 필터로 여과하여 최종 박테리오파지 배양액을 회수하였다. 분리된 박테리오파지를 "박테리오파지 SH9(Bacteriophage SH9)"로 명명하고, 2016년 12월 2일에 한국미생물보존센터 (Korean Culture Center of Microorganisms, 서울, 한국)에 기탁하고, 수탁번호 KFCC11708P를 부여받았다.In order to mass-culture the Salmonella sp. Specific bacteriophage identified in Example 1.1, the final bacteriophage culture recovered at 1.1 was diluted to 1.0 x 10 9 PFU / mL, mixed with the pre-cultured Salmonella sp. 1.0 x 10 10 CFU / mL, (Multiplicity of infection) of 0.1, and cultured at 35 DEG C and 100 rpm for 24 hours. After completion of the culture, the whole culture was centrifuged at 6,000 rpm for 25 minutes to obtain a supernatant, which was filtered with a 0.22 μm filter to recover the final bacteriophage culture. The isolated bacteriophage was designated as "Bacteriophage SH9" and deposited on December 2, 2016 in the Korean Culture Center of Microorganisms (Seoul, Korea) and received the accession number KFCC11708P.

실시예 2: 박테리오파지 SH9의 전체 유전자 분석Example 2: Whole gene analysis of bacteriophage SH9

박테리오파지 SH9의 전체 유전자를 분석하기 위해, 박테리오파지 SH9 배양액에 20 mM EDTA, 50 μg/mL의 프로테나아제 K, 0.5% (w/v) SDS를 가한 후, 50℃에서 1시간 동안 정치하였다. 동량의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합한 후 실온에서 12,000 rpm으로 10분간 원심분리하여 상등액을 회수하였다. 회수된 상등액을 동일 부피의 페놀-클로로포름-이소프로필 알코올 (25:24:1)과 혼합하여 실온에서 12,000 rpm으로 10분간 원심분리한 후 상등액을 회수하고, 상기 상등액에 3M 소듐 아세테이트를 전체 부피의 10%(v/v)가 되도록 가한 후 혼합하였다. 여기에 2배 부피의 차가운 95% 에탄올을 가하여 혼합한 뒤 -20℃에서 1시간 동안 정치하였다. 반응이 끝난 후 0℃에서 10분간 12,000 rpm으로 원심분리하여 침전물을 획득하였고, 차가운 70% 에탄올로 2회 세척한 뒤 에탄올을 완전히 제거하여 DNA 펠렛을 회수하였다. 회수한 펠렛을 최종 부피가 50 μL이 되도록 TE 완충액 (Tris-EDTA, pH8.0)에 용해시킨 후 DNA 농도를 측정하였다. 전체 유전자 분석을 위해 PacBioRSII를 이용하여 유전자 시퀀싱을 시행하였다(마크로젠, 한국). 시퀀싱 결과로 얻은 박테리오파지 SH9의 유전자 맵을 도 2에 나타내었다.In order to analyze the entire gene of bacteriophage SH9, 20 mM EDTA, 50 μg / mL proteinase K and 0.5% (w / v) SDS were added to the culture of bacteriophage SH9 and left at 50 ° C. for 1 hour. The mixture was mixed with an equal amount of phenol-chloroform-isopropyl alcohol (25: 24: 1) and centrifuged at 12,000 rpm for 10 minutes at room temperature to recover the supernatant. The recovered supernatant was mixed with an equal volume of phenol-chloroform-isopropyl alcohol (25: 24: 1) and centrifuged at 12,000 rpm for 10 minutes at room temperature. The supernatant was recovered and 3M sodium acetate was added to the supernatant in a total volume 10% (v / v). The mixture was mixed with 2 volumes of cold 95% ethanol, and the mixture was allowed to stand at -20 DEG C for 1 hour. After the reaction was completed, the precipitate was collected by centrifugation at 12,000 rpm for 10 minutes at 0 ° C., washed twice with cold 70% ethanol, and then the ethanol was completely removed to recover the DNA pellet. The recovered pellet was dissolved in TE buffer (Tris-EDTA, pH 8.0) to a final volume of 50 μL, and the DNA concentration was measured. Gene sequencing was performed using PacBioRSII for whole gene analysis (Macrogen, Korea). The gene map of bacteriophage SH9 obtained as a result of sequencing is shown in Fig.

도 2에 나타낸 바와 같이, 박테리오파지 SH9의 전체 유전자의 크기는 111,607 bp 이며, GC함량이 40%, 전체 ORFs의 수는 160개임을 확인하였다. 분석한 박테리오파지 SH9의 전체 유전자 서열을 서열번호 1로 나타내었다.As shown in FIG. 2, it was confirmed that the size of the entire gene of bacteriophage SH9 was 111,607 bp, the GC content was 40%, and the total number of ORFs was 160. The entire gene sequence of the bacteriophage SH9 analyzed is shown in SEQ ID NO: 1.

실시예 3: 박테리오파지 SH9의 형태학적 특성Example 3: Morphological characteristics of bacteriophage SH9

박테리오파지 SH9의 형태학적 특성을 알아보기 위해, 배양액을 0.01% 젤라틴 용액에 희석하여 2.5% 글루타르알데하이드(glutaraldehyde) 용액으로 고정하였다. 이를 Carbon-coated mica plate(ca. 2.5 x 2.5 mm)에 적가한 후, 10분간 적응시켜 멸균증류수로 세척하였다. 이를 구리 격자(copper grid)에 끼워 2% 우라닐 아세테이트(Uranyl acetate)에서 30-60초간 염색하고 건조시킨 후, 투과전자현미경 (Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000~400,000)으로 관찰하였다. 그 결과를 도 3에 나타내었다.To determine the morphological characteristics of bacteriophage SH9, the culture was diluted with 0.01% gelatin solution and fixed with 2.5% glutaraldehyde solution. This was added dropwise to a carbon-coated mica plate (ca. 2.5 x 2.5 mm), followed by washing with sterile distilled water for 10 minutes. The resultant was stained with 2% Uranyl acetate for 30-60 seconds, dried, and then observed with a transmission electron microscope (Tecnai G2 Spirit Twin, Bio-Transmission electron microscope, 120 kV, 120,000-400,000 ). The results are shown in Fig.

도 3에 나타낸 바와 같이, 박테리오파지 SH9은 200-240 nm의 크기로, 정이십면체의 머리를 가지며 수축성의 꼬리를 가지는 형태인 마이오비리대(Myoviridae)에 속함을 확인하였다.As shown in Fig. 3, it was confirmed that bacteriophage SH9 belongs to Myoviridae, which is 200-240 nm in size, and has a truncated dichroic head and a contractile tail.

실시예 4: 박테리오파지 SH9의 단백질 패턴 분석Example 4: Protein pattern analysis of bacteriophage SH9

박테리오파지 SH9의 단백질 패턴을 분석하기 위해, 1.0x109 PFU/mL로 준비된 박테리오파지 SH9 40 μL를 10 μL의 5X 로딩 완충액과 혼합하고, 10분간 끓인 다음 12.3% SDS-PAGE를 수행하였다. SDS-PAGE 수행 후 단백질 전기영동 결과를 도 4에 나타내었다.To analyze the protein pattern of bacteriophage SH9, 40 μL of bacteriophage SH9 prepared to 1.0 × 10 9 PFU / mL was mixed with 10 μL of 5X loading buffer, boiled for 10 minutes, and subjected to 12.3% SDS-PAGE. The results of SDS-PAGE and protein electrophoresis are shown in FIG.

도 4에 나타낸 바와 같이, 박테리오파지 SH9은 64 kDa, 58 kDa, 36 kDa, 35 kDa, 34 kDa 그리고 30 kDa 크기의 주요 단백질을 가지는 것을 확인하였다.As shown in FIG. 4, bacteriophage SH9 has major proteins of 64 kDa, 58 kDa, 36 kDa, 35 kDa, 34 kDa and 30 kDa in size.

실시예 5: 박테리오파지 SH9의 다양한 세균에 대한 감염성 조사Example 5: Investigation of infectivity of bacteriophage SH9 against various bacteria

박테리오파지 SH9의 다양한 세균에 대한 감염성을 조사하였다. The infectivity of bacteriophage SH9 to various bacteria was investigated.

우선, 박테리오파지 SH9의 살모넬라 하다에 대한 용균 활성을 확인하기 위하여, ㈜옵티팜에서 분리하여 보유하고 있는 살모넬라 하다 균주를 대상으로 소프트 아가 오버레이법을 수행하였다. 그 결과를 표 1에 나타내었다.First, in order to confirm the lytic activity of Salmonella spp. Of Bacteriophage SH9, a soft agar overlay method was carried out on Salmonella spp. Isolated from Opti Pharm Co., Ltd. The results are shown in Table 1.

Figure 112017127270012-pat00001
Figure 112017127270012-pat00001

상기 표 1에 나타낸 바와 같이, 본 발명의 박테리오파지 SH9은 살모넬라 하다에 대해 높은 감수성을 보임을 확인하였다.As shown in Table 1, it was confirmed that the bacteriophage SH9 of the present invention exhibited high sensitivity to Salmonella typhimurium.

또한, 박테리오파지 SH9이 다양한 세균에 대해서 용균활성을 나타내는지 확인하기 위하여 ㈜옵티팜에서 분리하여 보유하고 있는 균주와 미생물 자원센터 등에서 분양받은 균주를 대상으로 소프트 아가 오버레이법을 통해서 감염성을 확인하였다. 그 결과를 표 2에 나타내었다.In order to confirm whether bacteriophage SH9 exhibits lytic activity against various bacteria, the strains isolated from OptiParm Co., Ltd. and purchased from the microorganism resource center were examined for infectivity through soft agar overlay method. The results are shown in Table 2.

Figure 112017127270012-pat00002
Figure 112017127270012-pat00002

(+: 용균활성 있음, -; 용균활성 없음)(+: Lytic activity, -; lytic activity)

표 2에 나타낸 바와 같이, 본 발명의 박테리오파지 SH9은 살모넬라 하다 외에도 살모넬라 티피뮤리움에도 용균활성을 가지는 것을 확인하였다. 그러나, 박테리오파지 SH9은 Bacillus cereus, Pseudomonas aeruginosa, Listeria monocytogenes 등 살모넬라균이 아닌 다른 속의 세균에 대해서는 용균활성이 없는 것을 확인하였다.As shown in Table 2, it was confirmed that the bacteriophage SH9 of the present invention has a lytic activity in Salmonella typhimurium in addition to Salmonella typhimurium. However, bacteriophage SH9 showed no lytic activity for bacterium other than Salmonella such as Bacillus cereus, Pseudomonas aeruginosa, and Listeria monocytogenes .

따라서, 본 발명의 박테리오파지 SH9은 살모넬라균에 특이적으로 용균활성을 나타내는 박테리오파지임을 확인하였다.Therefore, it was confirmed that the bacteriophage SH9 of the present invention is a bacteriophage exhibiting a lytic activity specifically for Salmonella.

실시예 6: 박테리오파지 SH9의 pH 안정성 조사Example 6: Investigation of pH stability of bacteriophage SH9

박테리오파지 SH9의 pH에 대한 안정성을 확인하기 위하여, 다양한 pH 범위(pH3, 4, 5, 6, 7, 8, 9)에서 박테리오파지의 수를 측정하였다. 소듐 아세테이트 완충액, 소듐 포스페이트 완충액, Tris-HCl을 이용하여 각각의 pH에 맞게 조제한 후 990 μL로 분주하여 준비하였다. 박테리오파지 SH9 배양액을 1.0x1010 PFU/mL가 되도록 준비한 다음 배양액을 10 μL씩 취하여 pH에 맞게 조제한 각각의 완충액에 첨가하여 2시간 동안 상온에서 방치하였다. 방치 후 각 반응액을 단계 희석하여 소프트 아가 오버레이법에 의해 타이터(titer) 측정을 수행한 결과를 도 5에 나타내었다.In order to confirm the stability of bacteriophage SH9 against pH, the number of bacteriophages was measured in various pH ranges (pH 3, 4, 5, 6, 7, 8, 9). Sodium acetate buffer, sodium phosphate buffer, and Tris-HCl, and the mixture was divided into 990 μL. Bacteriophage SH9 culture medium was prepared to be 1.0 × 10 10 PFU / mL. Then, 10 μL of the culture solution was added to each buffer prepared for pH, and the mixture was allowed to stand at room temperature for 2 hours. After allowing to stand, each reaction solution was diluted stepwise, and a titer measurement was performed by soft agar overlay method. The result is shown in FIG.

도 5에 나타낸 바와 같이, 본 발명의 박테리오파지 SH9은 pH3-9의 범위에서 2시간 동안 안정한 것을 확인하였다. 이를 통해 박테리오파지 SH9은 pH 안정성이 우수한 박테리오파지임을 알 수 있다.As shown in Fig. 5, it was confirmed that the bacteriophage SH9 of the present invention was stable for 2 hours in the range of pH 3-9. This indicates that bacteriophage SH9 is a bacteriophage having excellent pH stability.

실시예 7: 박테리오파지 SH9의 열 안정성 조사Example 7: Investigation of thermal stability of bacteriophage SH9

박테리오파지 SH9의 열 안정성을 확인하기 위하여, 40℃, 50℃, 60℃, 70℃ 및 80℃등 다양한 온도 범위에서 일정 시간 방치 후 박테리오파지의 수를 측정하였다. 보다 구체적으로, 박테리오파지 SH9 배양액을 1.0x108 PFU/mL가 되도록 희석한 후 배양액 5 mL을 멸균된 튜브에 분주하고 30분, 1시간 및 2시간 동안 각 온도에서 방치하였다. 방치 후 각 반응액을 단계희석하여 소프트 아가 오버레이법에 의한 타이터 측정을 수행한 결과를 도 6에 나타내었다.In order to confirm the thermal stability of bacteriophage SH9, the number of bacteriophages was measured after standing at various temperature ranges of 40 ° C, 50 ° C, 60 ° C, 70 ° C and 80 ° C for a certain time. More specifically, the culture of bacteriophage SH9 was diluted to 1.0 × 10 8 PFU / mL, and 5 mL of the culture was dispensed into sterile tubes and left at the respective temperatures for 30 minutes, 1 hour, and 2 hours. After allowing to stand, each reaction solution was diluted stepwise, and the result of performing a tester measurement by soft agar overlay method is shown in FIG.

도 6에 나타낸 바와 같이, 본 발명의 박테리오파지 SH9은 30-60℃의 범위에서 안정한 것을 확인하였다. As shown in Fig. 6, it was confirmed that the bacteriophage SH9 of the present invention was stable in the range of 30-60 占 폚.

상기 실험으로부터 박테리오파지 SH9은 살모넬라균에 대한 특이성이 매우 높고, 살모넬라균에 감염하여 균 내에서 증식함으로써 용균 활성이 우수하고 물리화학적 자극에 강하다는 장점을 가지고 있음을 확인하였다. 또한 본 발명의 박테리오파지 SH9은 사람, 동물, 식물 등 세균 외의 다른 숙주에는 감염되지 않으므로, 항생제의 오남용으로 인한 항생제 내성균의 문제점, 식품 내의 항생제의 잔류 문제, 광범위한 숙주범위의 문제점을 해결할 수 있는 장점이 있다. 따라서, 본 발명의 박테리오파지 SH9은 살모넬라균에 의한 감염성 질병의 예방 또는 치료, 항생용 조성물, 사료 첨가용 조성물, 사료, 소독제, 또는 세척제 분야에서 다양하게 사용될 수 있다.From the above experiment, it was confirmed that bacteriophage SH9 has a high specificity to Salmonella, and has an advantage that it is excellent in lytic activity and strong against physicochemical stimulation by propagating in salmonella bacteria. In addition, the bacteriophage SH9 of the present invention is not infected with any host other than bacteria such as human, animal, and plant. Therefore, it is possible to solve the problems of antibiotic resistant bacteria caused by misuse of antibiotics, have. Therefore, the bacteriophage SH9 of the present invention can be used variously in the field of preventing or treating infectious diseases caused by Salmonella, antibiotic composition, composition for feed addition, feed, disinfectant, or detergent.

비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한 첨부된 청구 범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.Although the present invention has been described in terms of the preferred embodiments mentioned above, it is possible to make various modifications and variations without departing from the spirit and scope of the invention. It is also to be understood that the appended claims are intended to cover such modifications and changes as fall within the scope of the invention.

이하 본 발명의 박테리오파지 SH9을 포함하는 감염성 질병의 예방 또는 치료용 약학적 조성물의 제제예를 설명하나, 본 발명을 한정하고자 함이 아니고 단지 이를 구체적으로 설명하고자 함이다.Hereinafter, formulation examples of pharmaceutical compositions for the prevention or treatment of infectious diseases including bacteriophage SH9 of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

제제예 1: 의약품의 제조Formulation Example 1: Preparation of medicines

1.1 주사제의 제조1.1 Manufacture of Injection

박테리오파지 SH9 1 × 106 PFU/mLBacteriophage SH9 1 x 10 6 PFU / mL

주사용 멸균 증류수 적량Sterile sterilized water for injection

pH 조절제 적량pH adjuster

통상의 주사제의 제조방법에 따라 1 앰플당(2 mL) 상기의 성분 함량으로 제조한다.(2 mL) per 1 ampoule in accordance with the usual injection preparation method.

1.2 액제의 제조1.2 Manufacture of liquids

박테리오파지 SH9 1 × 106 PFU/mLBacteriophage SH9 1 x 10 6 PFU / mL

설탕 20 g20 g of sugar

이성화당 20 g20 g per isomer

레몬향 적량Lemon incense quantity

정제수를 가하여 전체 1,000 mL로 맞추었다. 통상의 액제의 제조방법에 따라 상기의 성분을 혼합한 다음, 갈색병에 충전하고 멸균시켜 액제를 제조한다.Purified water was added and the total volume was adjusted to 1,000 mL. The above components are mixed according to a usual method for producing a liquid agent, and then filled in a brown bottle and sterilized to prepare a liquid agent.

1.3 정제의 제조1.3 Manufacture of tablets

박테리오파지 SH9 1 × 106 PFU/mLBacteriophage SH9 1 x 10 6 PFU / mL

옥수수전분 100 mgCorn starch 100 mg

유당 100 mgLactose 100 mg

스테아린산 마그네슘 2 mgMagnesium stearate 2 mg

상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

한국미생물보존센터(국내)Korea Microorganism Conservation Center (Domestic) KFCC11708PKFCC11708P 2016120220161202

SEQUENCE LISTING <110> Optipharm.CO.,LTD <120> Novel Salmonella specific bacteriophage SH9 and antibacterial composition comprising the same <130> OTP1.33P-1 <150> KR 10-2016-0179216 <151> 2016-12-26 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 111607 <212> DNA <213> bacteriophage SH9 <400> 1 tatttctagg agacattttt ccaaaacaca tttctggaaa ttgctatcgg atttgcacac 60 tgtgtttata gaaatttatg gtcggatttg cacattgata ggagtctcca aagtttttag 120 gaacactgaa aatagcagca ttatttcggg ccgaaggccc atgattgcct gagtttttag 180 agccacagga attttcactt gtcaagcgat tttgcctcat tttgctaata aaatttcgct 240 ttatctccac taatctcaat tctagccaaa acatcccaca ttatccccaa aaacgcagca 300 aaccggtgct gaagcaccac taccgctgct ccttccgtag ttcctcccaa aatctcatcg 360 gattcgcact tccccccaaa cccacacaat cacacaccct ccaatcgaaa tcacaaaaat 420 cttgtcggat tcgcactact cattcctaga aatttcgtgt cgtatttgca catatacggc 480 tggatttgtg cgcagcacga gtctgcccga gtttttagga aaattcattg tcggattcgc 540 acactataaa aatatttccc atcggatttg cacactacca aaaattgtcg gattcgcaca 600 tacccgccag cacgtaatcc atggaggcaa atgtcggatt tgcacaaaag cgcagggcaa 660 aatgaaaatg ccctgcctgc cgaaatgcga atgataatga gattgattct catttgaaaa 720 gttgaatgcg aatgataatg attcgcgttt agaaagtgga atgagaatgg ttatcattta 780 cagaattgaa tgcgaatgag ttgcataatg ataatgattc gcatttagaa agggaagtga 840 gaaacactgc tatttaagtt atccacaggt ttattaacag catgatttga cttgacaaaa 900 tcctagcgat tttgaggcgt tatcaacaga cttattaaca ggttattcta ctgtataaat 960 atacagtata ttccagcagg agaataatct aatatagcct ttaaacgctc tgtaacgcat 1020 tctaacgcgt ttatttttgg aggaggtact ttaataaggg gaaatttgtt tgcaggcaga 1080 aaaggcgggg cgcaactatc ggccccgtta ttattagtct gctatgtatt ccttctcgta 1140 gtcgtcaaag atctggactg tttcggctgg cgtatatccg tatgaatcaa aatgcgtaca 1200 cgttgagact gaattgatcc acgcccctag cgccgccgta ctagctccgc ttttccgcat 1260 ttctaaaaat tgcgcgtatt gcccatcagt taaatgatgt tgcataatat cggtttcttt 1320 acgttctaca tatcgtaaaa ttcttacagc catgccattg ctcctagaat gagaatgatt 1380 atcaatatca ttgtgaagcc attaccaaat cgtaaacgct gggaatgttt cccagcggta 1440 ttattagatt tgtgactttg cgttttacgc caccagctat tcacggtaga cgctccaacc 1500 gtctagcttc cattcctcac ccttgcgaat attgaaaaac caatcattaa aattgtaacc 1560 atcttctgac atgatcacgc cgttttcttt catgtacaaa aattcatcgc tagtaaaata 1620 ttgattacta actttgaaac cttgtttcat tttttctagt gcttcatctt tagtcattgc 1680 gccacctttc gaaacgctgt ttcaatactt tcagcggttg attgaatata tgcgcctttc 1740 ataatcaggc gctccgtaca tactttgcgg aaaatgtcaa taaattcatt ttcgttttcc 1800 gctattaacg cttcccccat aacctgaacg tcacaagacc caaaagttaa aaattgaaac 1860 gctagttcac gggcgacaac ggcagaaatt ttataatgtg ctttgccctc atagatatta 1920 actacgcctt tataaactgg tgaaagctga tttaaatagt ttgctaataa tgccggacgg 1980 caattagtaa taaagcgatt gccctcataa attaccatta aaagatcctc gcgtcacgga 2040 taatcataat cactagcgtg atcaacattg cagcactaga cacgccacaa acaaaagtta 2100 acattttaac aattgccctt tccattataa taccccacga aatgcagcgt aaaaggtggc 2160 aaagtgttta atcgtttcgg ctaccaaatt gccacgcttg tcaaatttct tttctgtaac 2220 acggaaagcg tttttgttgc gtgctacata ttcgatttta caatctggac gctcgaacgt 2280 gttaacatcc accagcttaa aacctttgga acgggcaagg gaaacattct ttaacatttt 2340 tattcccctt taccagtcat acagcctaac aagtaagaaa accccgcgat acctgctcca 2400 ataataacca cgtgaacgcc gaaagctatt acaaacagcg ataaaatagc gcccaagata 2460 ctagccagaa tagatcgaaa aataaccatt ttattagctc ctagaaatta ggggataata 2520 tatccccttt aataattaca ttaaaacaat ttttgccgct tgctttttgg tgcaatggtt 2580 agctttcaat tcatccagca aaaatccggc ggtttgctgg tcaatcttgt cgttgtcgta 2640 aagctcctga atggtaacat aagttttacc gatcccaaat tcagaaacca taacgctgac 2700 aatagcggga acggataccc attccatacc gttaaaaatt gcagaagatg atgaatgcac 2760 cattttaacg cctcctagaa atcagggaaa attttcaatc caccgtttcg gtgtaaaggc 2820 attataacaa aaaaggccgc cgattaaggc gacctttaca aaactttaca ttacatatat 2880 ctgattattg catcgccgta aagctcgctt tcagatcgta acacttgctc gaaacgctca 2940 tattctagcg ccctgttttc ttttgggaaa tagcaagcgt tttctagtgc cttttgataa 3000 acatgcaatt cattatcaaa acgctggacg atccccaatt ctagcaaggc tttatatact 3060 tcatcctcac catgaccgcc gttaacaaat tgataacttt tcgtgaattg ccagtggtac 3120 gccgttgcgc ccacgtctgg attttctacc agattggaat cgtcacactc gcaatcatta 3180 gcagactcat cacaattctg acagatttca tgattgctaa aactggaggc gttttccatt 3240 gccatttcat gcaacggcgt ttcaatatcg ccagcgaaaa aatgttctac acttgcccat 3300 tgttcgccgt gggtattagt gtaacagtac actagtgcaa tgatcggatg atcaatatgt 3360 gatccggtta ctacgtcgcc atactttgac attaattagt ctcctattaa ttagcaggga 3420 aatatagaga atttgctaca tcgtaaaatc ctttattcaa gaaaattacg tcatcacgga 3480 ctatttcatc cattccataa tccagcaaag tttcatctac aaaaatacat agccagccat 3540 aaccacgatc aaagaaagca tgataattat tcgcgctttc tacatgttcc cataaaaagg 3600 gacgcccttg cttttcccat ataggaatat tgctttgatc gtctgaataa ttaacgtgtg 3660 ggctattcgg cattttttcg gctaattgtg ctaacatgat ttgattcagt tgcataataa 3720 ttagtctcct aacaattaac agggaaatag taaagcgggg aataatcccc gctagtaatt 3780 attattctgt ttcggtgttt tgggtaaagg tagaaatgtc aatacctttt accatttcat 3840 cgatcatttc agcaatattg aaactctgta ccatttccaa cattgccgct tccattgccg 3900 cgcctgctac cgctttcgct ttctggttag tcggggcgta ctggtcaacc gctacactca 3960 gcaggttagc gatcactaca cgaccaatcg gcgtatcaat ataaccttta atcataaacg 4020 gcgctttttt cgccgctact tttgtgattt gagtcagtgc aattttaccc gcttccagtt 4080 tagcagcgtt aacaacggcg gatttattag cggcaacgat agaagatact ttagacataa 4140 tatttccttt attttcagta gtttgcgcat ttgcgcttga agtttgagat tgtacagcct 4200 gttttgctac attgtcaagc gggagaagtg taaatccagc gtttgcaacg tttgattcat 4260 tcccatactc taaataatca ttaggaatca acatgttacc cgtagcgcgt tcaaaacaca 4320 atacgccagt tgtttcgatt gcgtcactat cagcacgttt aatggcgctt gtcaattcgc 4380 cccaaatact agatagtaaa ccttctacat cgctggaagt aataccattt acgcgagtag 4440 cgaaaaccat ttctttttct tgaacattgt aaccacgggc aatatagctt ttttcgtcgt 4500 gacctacaat gataaaatca atatcgcttc cccgctgatt tcgcatgatg tgatagtcac 4560 ggttgtaaag cactagaacg cgggtagggt ctagtaattg cgtgaaatca cttaaacaat 4620 aatgagtatc attattatct aaaatatcat cgtgaatctt ttcaaccatc gccgccgtat 4680 gttgtaggct tgctggattt gatccatcta gcaggttgcg caaactaccc ctaataatat 4740 tggtgtttgc tggtactgca attcttacag tagacatttc tttgtctccc tcgttaatcg 4800 gtggaaagat aataagcgtt ttctctttat aggggcaacg tttattttac ctttttattt 4860 tccttgctca attgctaaaa gtgtgctaat cgcgcgttta ctcatataac gctatataac 4920 gcttattgct ggctttactt taataaggcc accagcaaga aaacgccttg tagcgcgttc 4980 tagatgcctc aaaattgatt gcagatgtta gtgctaccta tttaccctgt atatttatac 5040 agtagaataa cctgtggata actctgtgca aaagtgctgt aaactcctct cattgtgtca 5100 agtcaaaaag tgctgttaat aaaactgtgg ataacttaaa tgatagtggt tctcactaac 5160 tgaaaaactc attaggtagc ctgctaatga ttttgatata atataataga gattaaatta 5220 ataacatgag gaaacaatta gatgactaat tataaagata ttgattatga gactgtaaat 5280 aatttatttt attatgatga aacatcggaa acatttctaa ggtggaaagt ttcacgcggt 5340 aataaaaagg caggtgatat tgccggatgt gatagtaccg gtggttacta tcgcgtttct 5400 attaataact ctgattacac ggtacatcgt atcatatggg tactatttca tggtgaaata 5460 gatagcaacc taacaatcga ccatattgat cgtaacaggc gaaataataa cattaaaaac 5520 ttatgccttg ctactcaatt gcagaatatt agtaaccgtg gttacaaatt gcgtgctaat 5580 ggtttgccta ctaatattta tcatgataag aaacgcccac gtgatagttt aggtcgtgat 5640 tatttgcgtg ctcaaattac aaatcctatt actaagaaaa agattagtaa gtgtggttat 5700 gatttagata cgttattatt atggttagaa ggggaaagag aagaatttaa aaagtcaatt 5760 aattaattta ttaaattaag tcatttggtc ttgattttta aaagtcaaga ccttttctaa 5820 ctttatttca attttgcggt tgttaaatat ttgttaagtc caatttaagg cgtctaaatc 5880 gctctctacg gcgttctaat ttctagctag gcaagtgcat tgccactatg ccgatctcct 5940 caccacgagg attttagatt ttgtcaagta ccctagagca aatataaatg aaaaaattac 6000 ttgctaaaat ggttgttttg gtgttattcg cgcgcccgtt tcattaaatt tagtgcgccg 6060 ctggacgttt gcgaatagtg gttttcttta cgtttcttta caaaaataag gttgcgccct 6120 tctcggtatt ctcctattat ttatcacgta gggcggcaat gacgaactac acggcgggga 6180 agtttaccgc catgctcttt aaaaactagg gaacatctta aagcctatcg gcggtaaact 6240 tatacattat aaggaatcga cgataatgat caaatcaaaa aacgctttag tgattagtgc 6300 gaaacctgct agcgcattag atagcagaac aaaaaatttg ttgacagtag cgcaacgatc 6360 tgtaaaatac agattcaagc aatacaagaa aggcaagcaa caagggctgg aaatggtgtg 6420 gcgtaatata atgattgatt tatataactc acatcaaatc gaaaaaagtt taaaaaacgc 6480 ttgacacgct agccgatagg ttttaagatg ttccccgtag taactgctct ttaaaaattt 6540 ggataacggt aatgtttagg ctaactccta gcggattata atctaatagc gtttcaaagt 6600 atcgaggcgc tattgtggaa taatccccaa taaaccggag tattaaaaat gcaaaacgtt 6660 attaccgcgc cgaaagtagg tcaatctgtt ttcattcctt ttgtaactaa aactgatgaa 6720 ttaaccggaa aagccgaacg tatcaaaggt gcggcgcttg atcctttcga taaaatcgaa 6780 gcggtttacg cggaaacgga acgtagcaac aacggcaagc cgatttatac cgtccgtgtt 6840 aaatctggcg acgttgtaaa ggtcattcag aaagatgata aatggcaagc tatcgcctaa 6900 tagcttatta acccgcgcct ttaatcggcg taaataaata aggctagcgc ctcatgttag 6960 attttcgata tattaaccat gtagtgtgaa caatgttatg gcacacaatt taaacttata 7020 tgattagtgt tacttttata ttgccctgaa atatgggcaa tataacgatt aacattagtt 7080 aatcataatc ccttaattaa taattgaacg gagtatttat tatgtctaac gttaaaaccg 7140 aaaaaaccgc aaaattctct tggaacgaag aaaacaccga aaaggctgtt tctatgtatt 7200 ctgaaatggt cgctaaaagc ggaattgaat ttgctaacag tgacggcttg aaagaaatcg 7260 ccgccgccgt tggtgctgct agtccggtgt ctgtacgttc taaattgacc agtgcaaaag 7320 cctaccagaa aagcgataaa ccgcgtaaag ttggcggcgg gtctagtgtc cgtaaagctc 7380 actatgtgcg cgttatggct aaacatgcca ttgacgctgg cgttattaaa gacgccgacg 7440 atctggcaag tctggaaagt gcaaaactgg aaacgctgga cgtcgtagcg caattgttag 7500 gcgtagcgga ggaagtaaaa caggccgcag gtgaataaat tttaactagc ggggaatatt 7560 ccccgctata atttcccttt gaccggagtt attaaaatga ttttctttcc tagtgaagcg 7620 tttattttag gtttatttat agttgccgct atatttatat ttaattacgg caaatatata 7680 atctctatta tcggcattaa ttcagcttat gccgctgata tgtttaaacg tcaagctaat 7740 aaacaaaaat atctggcgat tatattttta acgctggcta ttagttgcgg gtttagttct 7800 agtcttatgc caattctaga ataatattgc aaattataac gccttttaaa ttgtcctcac 7860 ttcaggcaag gcgttatatt tggcaatatt gccagtatta atatttatcc ctttatagga 7920 gttgatttag ttatgaatat ttccgcagaa aaacaagctc aaattttgaa aatggccgct 7980 gattttaatt tctatggtaa gcgtttacgc gctactaaat tggaagtttg cgacgatatt 8040 tcaaaagctg tttatgatac gccgaaacat tcgaccgcaa tttgtgactg gctggaagca 8100 aataagccag cgaaaccgaa agcggcaaaa gctgaaaagg cgattaaaaa tgatgatcgc 8160 cccgaagcgg cgggaattgt ttctagtacc gttgaacaat gggaagttaa atccggtcgc 8220 cgttttattc taacgtcgat tcaaaataat actttcccgc ataaaaactt tttagcaagt 8280 ctggaaaaat tcgctgaata ttgcggcgct gaattgctga ttagtaaatt catttataat 8340 aaaaatggat tccagaacgg ggaaggggca aacggtatta aatacgatcc cgcatttgat 8400 aaatatgttt gtgataaaaa cgtattttta aataaccgcc gctttgcttt catggctgaa 8460 attaatgttt taccaacggc agattatccg cttacaggat tcgcagaaac cgccacggct 8520 ttaaatattg aaggtctgac aattggtgcg gcaaaaatta ccgctgaaag tgtaccagcg 8580 ttaaaaggtg aagtagtgcg ccgtatgtat tcaaccggaa cggctacgct taaaaactac 8640 attcagcaga aagcaggtca aaaagctgag gctttacata attacggcgc tttgctggta 8700 gaatttgacg acgacggaga attttattgc cgccaacttg aaacaatgga tgaaagcgga 8760 atgttctatg atttgaatgt ttgcgctatg cctaccggat gttttgaaac gtccgatcat 8820 gttctaggct tgcaatatgg cgatattcac gccgaaaaat tagatgatga atgcgccgtt 8880 gcatcatggg ctagtgaaaa cagcttgctt gatattctta aaccgaaata tcaatttatt 8940 catgacgtgc atgattttac atcgcgtaac catcacaacc gcgctagtgg tgtattcctt 9000 gcgaaacaat acgccgccgg acgtgataaa gttattgatg atcttatcga tacgggtcgc 9060 gtattagagt ctatggagcg tgatttctct caaacaatca ttgtggaatc taaccatgat 9120 ttagctttat cccgctggtt agatgatcgt aacgctaaca ttaaagacga tcccgcaaac 9180 gcggaattgt atcaccgtct aaacgctgct atatacgccg ctattgctga aaaggatgat 9240 acctttaacg tgctagatta cgccttgcgt agtgtcgcag gttgcgaatt taacgctatt 9300 ttcctgacta ctgaccagtc gtttaaaatc gctggcattg aatgcggcgt acatggtcac 9360 aatggcatta atggcagtcg gggcaatcct aagcagttta aaaagttggg acgcctcaac 9420 actggccata ctcacacggc tagcatatac ggcggcgttt ataccgctgg cgtggcgggg 9480 tcgcttgata tgggctacaa cgtgggcgcg tcaagctgga cgcaaacgca tttaattacc 9540 tatgcaaacg ggcaacgtac tttgattgat ttcaaaaacg gtaaattctt tgcataatta 9600 aatttaatcg gcggttgaaa tataccgcca ttaataaaag gtttattaaa tgaatattgt 9660 attacctaaa acactggata aaaagcagta tataacgccg gatgatatgg aatgcggcaa 9720 ggtataccaa ttcgattata tgttggctag tccgacgaat acaggttgta ttattgcagt 9780 tgatacccgt gataatgacg tattaacgtt taatgattat tctgacttta tggaatggtt 9840 agatgatcaa agtattacta ataacggcga attaacgccg tatcaattaa caattgcaat 9900 taaataaatt aatcccgtaa ttaaataatc tggagtcata aacatgaaaa accaaaacat 9960 tccttttgat cgtgctactt cttccatcgt tctagtttat tctaatggcg aacgttatca 10020 cgtcgaggcg gggcaagtta atttcgattt gctgaattat aacgacgctt tgcaggttac 10080 tacctttgct tatgataccg gcaaagtggc aagccataca aaagcaaaag gcgtttacgc 10140 tgatactttg acagtgcaaa caattattat tgatgcgctt aaatccggtc tagcgtttgc 10200 agttgttaag ccgtgtccgg tctgcaatcc agaagctagc gcaatggttt acacttgcgc 10260 gggaattcgt tctagcgtac atggtgaaga tcttagcttt atcggtgacg cgctagcgta 10320 tggcctcact aactaatagt taattgaaca ttgccagcta atctaatata attagctggc 10380 aagattgaat taacctaaat cccttaaatg gagaatgtaa caaaatgaat aaagtcaata 10440 tgaatattac ccgcgctttc ccgcatattt cccgcgttat gatttgggat ttagacggaa 10500 cgattataaa ttctttccat cgtgtagcgc cttgctttga tgataacggg aatttagatt 10560 taaataagta ttcccgcgaa gcctgcaaac atgatttaat tatgcaagat tctttattgc 10620 cattggttga atatatgcgc caatgtatga atgatgcaaa tactttaaat attatttgta 10680 ccgcgcgctt aatgtctaaa agcgattatt tttatttacg caaacaaggc ttgcgagggc 10740 gcggaaatag taatatccgc gtattttccc gcgatacact gcacaaatac tttgaagcgg 10800 ataaggttag cgaaatatac cacagcaaag atgctgtata taaatcttat tactttgaac 10860 tgtttaaaca gctatatcct aacgctgatt tcacgatgat tgatgatcat aaaggtgtat 10920 tatcggcggc ggcaagtgct ggatttaaaa cgctggacgc tcaagccgtt aatgatatcc 10980 tatcaatcgg cgtaacattg atcggtgaaa cctttattga tgaatcgctt gatgatgata 11040 acgattatca attcctagcc gaacgtttaa aaatgtgttg ggaaggtatg accgaggaag 11100 aacgcgcgga atatagttgc agcccgcaac aattcattga taaattaaaa gttgcataac 11160 aattaaaaat aaaatagtca cactatgtta tcatcacata gtgtgactat tagcttgcta 11220 agtaccgggg cggtaatgag actcattctc atctagggaa cctctgggca caccttcatg 11280 tgtaatctta tgaaatttcg agaattggta attagcgcta acttaggaag aacaccggtc 11340 tactggcttg tctcctgatt ctgacatacg aaacctcctc tgcgacgccc gagatccatc 11400 cgaccactct cgctcaaact ccgcacgttt ctccgcaaac ttccgctccc tttccgaaag 11460 ctcccgcgat actcccgcaa cgtattcacg atgcttgtta atagccccga gtgttccgaa 11520 gattcccgca agtaccccga taatgaagat agctacaaaa ctaaacatta cttgtctccc 11580 cgcagcttac gttcgatacg agctaagtgg ttatctaaga tgcactgccc tataattacc 11640 acaactagta aagtaataat tactacttcc attataacca ccccgccata gctgctaacc 11700 aaaagagtac aaagataaag aataacacag tcccgaatcc gaaactattt atccaacttt 11760 cgtacacttc cccgcgtctg ttctttcttc tgttgtcatc tgacatctct ttgcttctcc 11820 tatagtcttg gtatttggaa taccctagca attgcacgaa ccattacgac tgtcctgatt 11880 agatccatta cttcgtcgaa gccggattct tctagtaaga aggatgttcc catcacgtct 11940 tctattgtat cttctatgac gtcttgaatg atttcatcaa ttgcatcatg tattgctttt 12000 tattcctggg atttatttca aagttaacag gttcctgaaa ggacttttct tcatcttgca 12060 ttgtgtttgc tacctgtacc tcatcccaga aataaaaatt tttgtttact tcgagatctt 12120 tttactttat actaattaca agtgaccaga ttgttctagt ttaaatttgc aatcaccata 12180 tttgagatca aactgtgaca aatcccacca cccaaactta gtgcgtaact gagttttagg 12240 gaatcggtag cccacagtac gtgcttctcg aatatctaaa gcgtagtgtt cttcaccaac 12300 atgaccataa gacttcacat atgccttaca aaatggctcg ggagctggca cacaacccgt 12360 caacactact gctgcaacta cgatggctag taactttttc ataatcgttc ctctgtttaa 12420 tttataaata tattatagca aaatagaggg atctaagcaa atacattttt aaagaaggag 12480 attgtatgag ttttttcgca ggaaaattta gtgatggtaa aactgtatta tctctaaacg 12540 ctgctaatgg tggtgatata aatcagcact actctccaaa tactaatagt atcttccact 12600 cagatatgcc atttgtccta gttgatggga cttacgaggc cgctctaggt gatgcaggca 12660 atgggtattt tgttactcag atgccttggg atattataaa cattaaatcc aatgatcctg 12720 gtagagttat actaactgct attgagataa acggtactca tcgtggcttt ctgaatggta 12780 cacaatctca ggttggccaa ttcttaacat tcttcgaaga tcaaccacgt gctggtgctg 12840 aactggctat aacatctgct tttgcaatcg gtgatagttt agctcacggt acttatatat 12900 acaatggtgg tcttggtcat gaggaatcta ttgctaggca aggaactggt ggtactttac 12960 tacaatcttc cggtttccag atctttagac ctggtggtgt ttccgcaggt gaagctattt 13020 ccagagcctg gagtcttatg ggttttccaa ctggtgtctc tactgttcct ttagatggtg 13080 gtaacgcaga ttattgggaa cctaactggc aggcgcctat tggttctgct ggtagaggcc 13140 atgattggtt ttatgtttgt agttctaata ttcgtggatt tgtaggtaaa aaaggggaga 13200 taccttctaa tgtaaacatt aactaccaat ctcctgcata cccagaaaaa atatatgttt 13260 gtagaggttc tacttctaat atggctgctc agtctgctag aaaggtatat gtacaggact 13320 ggtataacgt tacgccaact aaggttattt ggtatgtgct aaatctgcgc tattctaatg 13380 gtagtatggg agtttctggt aatccattta ccggttcaga tatcttaata tctccgtcta 13440 attttactat taaaggtgtt agcctaccaa atacaggcta taaatttatt aaccagaatg 13500 ctttcggaaa cttaggctat cgccctgata tggagtatgt tggtaataac gctgcgtata 13560 ccggagtttt tggtgataat actgcacgat gtgaatttat cggctctagt aatggcggat 13620 tgtggtctcc tgttaactat gggggtgcag cttctcaaat tagcttatat aaatttggtg 13680 taggtaaaca atggtatgta gatactaata ccaactccat tggtaatgaa catggacctg 13740 tgtggagtcc tagtactgta cccctcaggt tattcccagg caacgttgca agtacttatg 13800 taggggacga tataactcca tcctaccctg gtgctggtga cttctacaca cctttggcca 13860 cagtttggtt gggattacca aatgctcact ctactgttat attaactact gaagttatta 13920 tgggtaatct taatactgct ggaatgcctg tgcgtactta cggcggtagc gcttggcagg 13980 tacaaggtag acgtcaacaa agttatacag ctggtgatgg tgtattccac cagattctta 14040 ctctgccacc tggatatcta gtaccttatc acagtacaac ttcctatagt tatacacaga 14100 cttgggctag taggcctgat gagtttgctt tccgtaggaa cggatatatt tatactatta 14160 aaaaccttgg taatggcaac gttgagttgg gtgttatcat tcacgctgca gaagcggcag 14220 ctgttttctt gccaagacta cgtgtaacag ttcaacgcct aacctaaagg aggatatatg 14280 gcaaatgatg tattagttcc cgatctcatg tctcccgaag gcatggatgt tattgaagcc 14340 tatttacagt gcggtagtga tgttcctgcc gccgctcgta gtcttggaat gtccgagatt 14400 gcattccgtg atataatgaa tcgtggggaa gttaaaaact atctcaacga tatctttatg 14460 gaaagtggct tccgtaatcg tgaccgctta ttcggagttc ttgacgaagt tatcaagcgt 14520 aagctggaag aacttgaaga aactggcatg ggttctgacc aggatatcat ggatattctc 14580 tggaaggcgc acaagatgaa gatggaagaa atgaaaatga tggtagaact ggagaaagtg 14640 aaggcagcag cccgtactcc ggctaatcag actaatattc agaataatat tattgctgga 14700 agtggtgacg aaaactatat gaatcttatt accgctctag caggaggagg taagagataa 14760 tgaaagtctc tagagactat attaacacca cagatgttat tgactttggt attgacaaac 14820 gattcttccg tctgccagtc tctagcattc tagctcaaga ggggattaca cctaatgccc 14880 ctcagattgc aatcattaat gcgctggaag acccaagaca ccgcttcgtg actgcatgtg 14940 tatcacgtcg tgtaggtaag tcattcatcg catatacact tgggttccta aaacttctgg 15000 aacccaatgt gaaggtgctg gtagttgcac ctaactactc actggctaac attggttggt 15060 cacagattcg tggtcttatt aagaagtatg gattacagac tgaacgtgag aacgcaaaag 15120 ataaagagat tgaactagcg aatggttctc tctttaagct ggcttctgcg gcgcaggctg 15180 actccgcggt tggtcgttca tatgacttca tcatttttga cgaagcagcg atttccgacg 15240 ttggtggtgc agctttcgat attcagttac gtcctactct agataagcca aactctaaag 15300 ctctgtttat ttccacacct cgtggtggta actggtttaa agagttctat gagaaaggat 15360 ttaacgaaac gctacctaac tgggtatcta ttcatggtac atatcgtgat aacccacgtg 15420 ctgatctgaa cgatatcgaa gaagcgcgtc gtactgttag taaaaactac ttccgtcaag 15480 aatatgaggc tgacttctct gtattcgaag gtcagatctt tgacaccttc aatgctatcg 15540 aacatgttaa agacctcaaa ggtatgcgtc acttctttaa agaggatgag gctttcgaaa 15600 cattacttgg tatcgacgtt ggttatcgtg accctacagc agttcttact atcaagtatc 15660 actatgatac tgatgtttat tacgtactag aagagtatca gcaggctgaa aagactacag 15720 ctcaacacgc tgcttatatc caacactgca tagatcgtta tagtgttgat cgtatatttg 15780 tagactccgc tgctgctcag ttccgtcagg acttagcata tgaacatgaa atcgcttctg 15840 ctcctgctaa gaaatctgtc ctagatggtc tggcatgcct acaagcactg ttccagcaag 15900 gtaagatcat cgtagatgct tcatgtacct cattgatcca cgccctagcg aactacaagt 15960 gggacttcca ggaaggcgaa gagaaattat cacgtgaaaa accacgtcat gatgcgaact 16020 ctcacctttg tgacgcactg cgttatggaa tttactcaat ttcccgtggg aaataaataa 16080 gtataggatg ggatactact cagttggtat cccattcctg cattttaaaa tcccctttac 16140 aaattcgaca tgattatgta tactatattc atacagttga ggagaatact gtggcaggaa 16200 atgtaaaata taaacgtgat gctatatcac tcatgcgaga cggtataaaa agtcaatata 16260 aaaaagccga tcgctgcgaa atctgtggtt gtggtgaaga attagagctt catcattacc 16320 atacagtgtc tctactagtt aaaaaatttg ctaaagaact ccaattggat ttcactgacg 16380 aagaaacagt cctctcaaat aggacggcat tctatgaaag atataggcat gagctagtag 16440 atgacactgt taccctttgt gtccaccatc accaactatt acataaggtg tacactaaag 16500 aacctcccct tttctctgct aataaacaga aggcatgggt tcttaaacaa aaggaaaagc 16560 tacagaatcc tcaagaaaag acacaagtca agactgaaac taaatcagga ttcgcaaggt 16620 tcttataatg ggtttaaaaa gctggattac tgaaaagcta aatccgggtc aacgtattat 16680 aagagacatg gaacccgtta gtcatcgcac taaccgtaag ccatttacca ctggacaagc 16740 ctacagtaag attgagattc tcaaccgaac tgctaacatg gttatcgata gtgcggcgga 16800 gtgttcttat actgtcggag ataaatataa tattgtcacg tacgctaatg gcgtcaaggc 16860 aaagactcta gacactctct taaatgtacg acctaatcca ttcatggata taagcacatt 16920 ccgtagactt gtagtcactg acctactttt tgagggttgt gcatatatct attgggatgg 16980 cacagcactt tatcatgtcc ctgctgcact aatgcaagta gaggcagatg ccaataagtt 17040 tatcaaaaaa ttcatattta ataaccagat agactatcgc gtagatgaga ttatctttat 17100 aaaggataac agttacgtgt gtggcacaaa ctcccaaatt tctggacaat ctcgtgttgc 17160 tacagttatt gactctcttg agaagcgttc taaaatgctt aactttaaag agaagttcct 17220 cgataacggc acagtgattg gtcttattct tgagacggat gaaatcctga acaagaaatt 17280 gcgtgagcgt aaacaagaag aattacaact cgattataat cctagtactg gtcagtctac 17340 tgtcctgatt ctagatggtg gtatgaaagc aaaaccgtac tcccaaatat cctcttttaa 17400 agatctagac ttcaaggaag atatcgaagg atttaataaa tccatctgtc ttgcctttgg 17460 tgttccgcaa gtgttacttg atggtggtaa taatgctaat attcgaccaa acatcgaatt 17520 gttttattac atgactatca ttcctatgct gaacaagctg actagttctc ttactttctt 17580 ctttggctac aaggttactc ctaatactaa ggaagtagct gcactgacgc cagataaaga 17640 agctgaggct aaacatttaa cctctttggt taataacggt atcatgactg gtaacgaggc 17700 tcgtgaagag cttaaccttg aacctttaga tgatgagcag atgaataaga ttcgtattcc 17760 tgctaacgtt gctggttctg caacaggtgt atctggtcaa gaaggtggta gaccgcaagg 17820 cactgccgat ggggataaag aatgattgat tatgatggtc taaaggccat ttttggtgaa 17880 aagctgccag aatctcatat cttctttgct acggttgcag cacacaagtt cgttccaaac 17940 tacgctactc tacgtaaaga gtttggaatg accactgcac ataccaaccg caaggtttgg 18000 aataagttca aagaagcata cgaagttact gctccggttg ttccggctct agctttcacg 18060 aagaatctgg ctaaaactct agctgttgat actggtgcag caattaatct gggcgtaaca 18120 gttactggtg gtactgcacc atatacttat gcttggacta aagacggttc tccacttagt 18180 ggtgttactg gccctaattt caacaaagct acagctgctg cagaagatgc tggtacttac 18240 aaagttgttg taacggatag taaatctact tccattactt ctaatgagtg tgtaacaact 18300 atcaaccctg cgccggaggc ttaataaatg acaaatgctg ctattgacta taacaagtta 18360 aaatcagcac cggttcattt agacgcttac attaagtcta ttgatagcga atccaaagag 18420 ggtgttgtaa aaatccgtgg attcgctaat acaattagta aagatcgcgc tggtgacgta 18480 attcctgctt cggcatggaa aacgtctaat gcacttgcta actacatgaa aaacccgatt 18540 attcttttcg gccacgacca tcgtcgtcca atcggcaagt gtattgatct taaccctact 18600 gaaatgggtc tcgaaattga atgcgagatt tatgaaagtt ctgatccagc tatcttctca 18660 ctaattaaaa acggtgtact gaaaaccttc agtatcggat tccgctgcct agacgcagaa 18720 tgggatgaag ctactgacat atttattatt aaagatttag aactatacga agtttcggta 18780 gtttctgtac cttgtaatca ggactcaacg ttcaacctcg ctaagagtat gaatggccac 18840 gattacactg aatggcgcaa atcttttact gcaacaagtt ctaaggctgt cccagctcaa 18900 gaacgtaatc tttctgaact agaaaaactt gcgatagctt taggctacgt taaagaataa 18960 cggagaatta tttaaaaatg actattgata ttaataagct gaaggaagaa cttggtctgg 19020 gtgatctggc taaatctctg gaaggtctga ctgctgctca gaaagctgcg gaagctaagc 19080 gtctgcgtga agagcaggaa gaaaaagaac tggctcgtat gaacgaccta gtatctaaag 19140 ctgttggtga agaccgtaag cgtctggaag aagctctgga tctggttaag aacctggatg 19200 agaagtctaa gaagagcgca gaactgtttg cacagactgt agaaaagcaa caggaaacca 19260 tcgttggtct tcaggacgaa atcaagtctc tgttggcagc tcgtgaaggt cgttccttcg 19320 ttggtgatag cgtagctaaa gctctttatg gcactcagga tgctttcgaa gatgaagttg 19380 agaaactggt tcttctgtct tatatgatgg agaaagacgt attcgaaact gaacacggta 19440 aggctcacgt taaagctgtt aacggttctt cttccgtttc catgtctagc gaagcatacg 19500 aaactatctt ctctacccgt attatccgtg acctgcagaa ggaactggta gttggtgcac 19560 tgttcgatga actgccaatg tccagcaaaa tccttactat gctcgttgaa ccagaagctg 19620 gtcgtgctac ttgggtagat gcttccaaat tcggtactga cgaaactgtt ggtgacgaag 19680 ttaaaggtca actgactgaa atcagcttca aaacttacaa gctggctgct aaatccttca 19740 tcactgatga aactgaagaa gatgcaatct tctccctgct gcctctgctg cgtaagcgtc 19800 tgatcgaagc acacgctgtt tctatcgaag aagctttcat gtccggtaat ggtactggtc 19860 agcctaaggg tctgctgaaa ctggctgctg atgatggtgc taaggttgtt accgaagcta 19920 aagctgacgg ttctgttctg gtaactgcta agactatctc caagctgcgt cgtaaactgg 19980 gccgtcacgg tctgaaactg agcaaactgg tactgatcgt atctatggat gcttactacg 20040 atctgctgga agatgaagaa tggcaggatg ttgcccaggt tggtaacgat gctgttaaac 20100 tgcagggtca ggttggtcgt atttacggtc tgccggttgt agtttctgag tacttcccag 20160 ctaaagctgc ttccgctgaa ttcgcagtta ttgtttataa agataacttc gtaatgccgc 20220 gtcagcgtgc cgttactgtt gaacgtgagc gccaagctgg caaacagcgt gatgcatact 20280 acgttaccca gcgtgttaac ctgcaacgtt acttcgaaaa cggcgttgta tctggtgctt 20340 acgctgcatc ttaatacagg cttttcagcc gataaggaga gcttcggctc tcctttttta 20400 ttgggaaaaa taaatgcaaa tcatcactgt tgaagattat aaactatatg ggaatttaaa 20460 acgtcccgat atggaaactc aggttgagat gatgattaaa gctgccaata cgctgattac 20520 aagccttcta ggtatggatg atgcagatgc ggtagatcaa ttaattacca caaaacctag 20580 ccgtaggaaa tatttcctga gttctccttc agccacatct attacaaaga tgactattaa 20640 cgagaatgaa atagatccgg aacagtttaa actgtatcct gatggtgtta ttctccttaa 20700 gtttaatcct cctgaaggat atatggatgt agagtatact caaggtggtt tcaatccaat 20760 gcctgaagat cttaaactgg cagcatgtct gttagtagat cactggcata agcaagatta 20820 tcgtcaagcc aagactattg gtggtgagac agttacgttt aacaatacta agtctggtat 20880 tccagaacat attcgtacaa tcatcgaagt ctataggaga gtgtaatggc tctttctgat 20940 ctagctagac aaattattaa agagcagcta gatactgcag gacgatctga gaataataag 21000 aatactgttg tgtatacagt tgagacaggt ttgaaagatc ctacaagaga tggtacagtt 21060 gcacaggtat ctttcaaatt ctcgaaacca gtatcacaag acttattaaa tgttaggaca 21120 gcatctatac tgaaggctgt ttcttctaac ttagatcttt ctggtgattt aggtgctcta 21180 gagaatctaa ttcaagcaac cgcaggtaaa cgttccgccg ttggtaaaaa acgttctacc 21240 ggtagagttc aggtaaactt tggagatcct agagatactg aagatggcta ttctggtgca 21300 gtaacaggtg cttccggtcg tttcgtatcg aatagtaaca tgcgtgctat tcttgagata 21360 gtagctaagg aatacttaat caaggatatg aaaaaagcag gggcaccatt gaagttcaga 21420 actggtcgct ttgctaactc cttaaagatt aaagacgtta tgctacgtga tgcagaaact 21480 agtaaaggtg ctccagagct gaatgtgacg tataactata tggttcgtcc ttactctgtg 21540 ttcaaccccg cagtttctac atatcgaaga ctttctctac gaccttaccc tggcgctcga 21600 aaccctcaaa aacttattgg ggaagcaatc gcaaaagctg caagagacct gattcactct 21660 agatataaaa tcaaggttaa tcaaggaacc tagtaaatgg atcacagaac aagtattgca 21720 caagcagtgg ttgaccgaat cgccacacaa atggatggtt cacaacctga cgagtatttc 21780 aataaccttt atggaaacgt ttctcgtcaa acttataaat tcgaggaaat ccgagaattt 21840 ccttatgtag cagtccatat cggaactgaa actgggcagt atcttccctc aggccaacag 21900 tggatgtttt tagaacttcc aatcctcgtg tatgacaaag agaaaacaga tattcaagag 21960 caacttgaaa aactcgtagc tgacataaaa accgtcattg acacaggtgg aaatttagaa 22020 tatactgtta gtaaacctaa tggatcgacc ttcccatgtg aggcaactga tatgagcatc 22080 acctcagtag ctacagatga gggtctactg gccccatatg gtttagcgga aataaatgta 22140 acagtgaggt atcagccacc tcgtagggca cttcgcagat aagttacaga ttaggagaat 22200 ataaataaat gtctttacaa ctattacgta atactcgaat cttcgtgtct acggttaaga 22260 ctggtcacga taagaccaac acgcaggaga ttctagttca agatgatatt tcttggggtc 22320 aggacagtaa ctcaaccgat attactgtta acgaagctgg tccgcgtcca actcgtggtt 22380 caaaacgttt caacgactcc ctgaacgcag ctgagtggag tttctctact tacatcctgc 22440 cttacttaga taagactact agtaagcaga tcgttccgga ctacatgctg tggcatgctc 22500 tttctagtgg taagcctatc aacctagacg gtgatacagg tgctcacact aacgaaacta 22560 acttcatggt taactttaag gataacgcat accacgagct ggcaatgcta catatttaca 22620 tccttactga taaagcatgg agctacatcg attcctgtca gatcaaccag gctgaagtaa 22680 acgttgatat cgaagatatc ggtcgtgtaa cttggtcagg taatggtaac cagctgatcc 22740 ctctggacgc tgctcccttt gatcctgata ctgtgggtat tgatgatgaa acttacatga 22800 ctatccaggg ttcttacatc aagaacaaac tgactatcct caaaattaag gatatggata 22860 ctggtaaggc atatgacatt ccgatcactg gtggtagctt tacaattaac aacaacatca 22920 cctatctgac tcctaacgtc atgtcccgtg ttaacatccc aatcggttca tttactggtg 22980 cattcgaatt aactggttct ctgaccgctt atcttaacga taaagctcta ggttctatgg 23040 atctgtataa agatctgatt aagaccctga aggtagttaa ccgtttcgaa atcgctctag 23100 tacttggcgg tgaatatgat gatgagcgcc cagccgctgt tctagttgct aagcaagctc 23160 acgttaacat cccaactatt gagactgacg atgtacttgg tacttctgta gagttcaaag 23220 caattcctac tgatctggat actggtgatg aaggttatct tggtttctct aataagtaca 23280 ccaaaactac tattgcaaat ctgattgcaa ctggtgacgg tggtaaagca gctccggtta 23340 aggtaacagg tattactgtt aaatccgcgg gtgatgcagc ttccgttgat aatagtgcaa 23400 ctcttcagat gactgtagat gttttgcctg ctgaagctac taataaggaa gtaacttggt 23460 ctatttcctc cggtactgct gctactattg atgcaacttc tggtttactg accgctgatg 23520 cttctaagac tggtgcagtt actgtgaaag ccgtagctaa agatggttct ggtgtagaag 23580 gtactaagag cattacagtt actgcttctg gtggctaatt aaatgtacta ctctctaatg 23640 agggagtcaa aagttatagt tgagtatgat ggcagggctt atcattttga tgccctgtca 23700 aattacgata tcaatacgtc ctatgaggaa tttaaaactc ttcgtaggac tatccatcgt 23760 agaaccaact atgctgactc tattattaat gcacaaactc catcttcaat atcgctggct 23820 ataaatttta gtaataccct tactgaagct aacttctttg aatggatggg ctttgaccga 23880 gagggtaaca cattcttact accactttat agtacaaata ttgagccaac aatgtttaat 23940 atatacatag ttaataaaga taataactgt gtgtactttg agaattgcta tgtatctact 24000 gtagactttt ctctagacaa aagcgttcct atcctaaacg taggaattga atctggtaag 24060 ttctctgagg tgtctacttt tactcctgct ggttctattg ttcagggtga agttatgtca 24120 tatagtcctc ctagagtatc taccaacagt aatgttttac cagctctttt atccgcttct 24180 atgtcattcc agcaacaatg ttcttggaga gaagataaaa gcgtatttga cataaacaaa 24240 atatataata ataagagagc ttatgttaat gaaatgaatg cttcggcaac aattgcattt 24300 tactacacta agcgatttgc tggtgatatg ttttataata ttgaacccga aactgacata 24360 ccattaacca tccgaaatga acatatttcg attgactttc cttcagcacg tataacaaaa 24420 cgcctacaat tctcggatat atacagagtt gaatgggatg taatccctac tgcagactct 24480 gatccggtaa gaatcgactt ctttggagaa ataaagaaat gatcaattta aaagatatta 24540 cccttgaaac tcgtactatt gagctggctt acccaggtat gcctaacttc aagctgcaac 24600 tgaactacat gtctcgtgct gcatctaagc gtgttatctc ttccgctaag cgtgatgagt 24660 gggttaacgg tactatgatt caggttcagg acgatgataa gtttattgaa gctttcgttg 24720 atactgcaat tgctggctgg actggcttga cgattggaga tgttgagaag ctgatgctta 24780 tcgaaactga agctgatcct actactgaag ttccgtttag tcgcgataac gctatcatgc 24840 ttatgcagaa ctctgctgcg tttgattctt ggatcaacca gacagtgttc cacttagaca 24900 cttttcgtag ctcaaaagcg taagcagtta ctagatgctg ttgccgattt tgcagataaa 24960 tgcattaaat cctcagcatc taaaatgaca aaacaacaat atttaatgct ttgcgagtct 25020 ctaggtattg aacctgatcc taaggcaatg cctgttgaac tcgaagattt tccacctatt 25080 gtatctacta gtatgaatat ttataacagt cttattgact gtttcatacc aggtgacttt 25140 cctatcttta taggaaaaga caaaagtgca ttaggtgttt tatttgatat ctatgggatt 25200 actgatccta tagagaaaga gtttgttcta cacatcatca atatatttga ttcgaaagct 25260 gtagatgcag ctcgtaagag agccgagaag tatagtaaac ctaatggtcg taccccgaac 25320 attaaaccac atgctagatc tcgagctcag taaaagtttc ctccaatggg cgttccacga 25380 tgaggcttgg ctctgggtga tttgcccaga gcctttttta ttgggaaaaa taaatgactg 25440 ataagctaat acgagaacta cttatagatg taaagcagaa aggggcaacc cgtactgcaa 25500 aatctattga gaacgtttcc gatgcgttag aaaatgctgc tgccgcttcc gaactgacta 25560 atgagcagtt aggaaaaatg cccaaaactc tttactccat tgagagggcg gcggatagag 25620 cagctaaaag tctaactaaa atgcaggcta gtagaggcat gactggtatc accaaatcta 25680 tggatagtat tggtgctaag ttagatgacc ttgctattgc tatgatcgag gtatccgata 25740 agctagagtc tggatttaca cacgttggta aatctgttaa ggcgatgggt aacgatgtag 25800 ctgctgcaac tgaaaaagtt caagatagat tatatgatac taaccgagca ctaggtaata 25860 ctgctagagg ctttaatgat acaactaccg ctgcaggacg tgctagtcgt gcaattggta 25920 atacttctgg ttcagcacgt ggtgcaactc gtgactttgc agctatggct aaagtgggtg 25980 gtagtttacc tcttatgtat gctgctatcg cgtctaacgt gttcgttctg caatctgcat 26040 tcgaacaact taagatgggt gaccagttaa accgtcttga gaagtttggt actattgttg 26100 gtactcaaac aggtactcct gtacaaactc tagctagatc tctacaagag gctgctggtt 26160 atgcaatctc cttcgaagaa gcaatgagac aggcatcctc tgcttctgca tatggattcg 26220 acgctgaaca acttaataaa tttggtctag tagctcgtcg tgctgctgct gttcttggtg 26280 ttgatatgac tgatgcactt aaccgtgtaa tcaagggtgt atctaaacaa gaaatcgaac 26340 ttctagatga acttggtgtt actattcgtc ttaacgatgc ttacgcagac tacgttaaac 26400 agttaaatgc tgctaacaca ggtattacat ataacgttaa tagtctctct actttccaga 26460 agcaacaagc atatgctaac gcagttatcg ctgaatctac taaacgtttc ggctatcttg 26520 atgatgtact tcgtgcaact ccatgggaac agtttgctgc taatgctgat gctgcactga 26580 gaaaggttca acaggctgct gctaagtatt tagggccagt aattgattct atcaacgcag 26640 tgttctatac ttctcaagca tctatctcgg cctctgctgc tagagcacag gaagaaacta 26700 accgtcagat tgacccaact aacgttggtg ctgtagcttt aagtctttcc gcttctgagg 26760 aaggatataa taaagctctt gatatgtaca aagaatctct agaaaagcgt aataagttaa 26820 aagctgatct agataagcgt atgcagcaag cagatgcatc tacagcaggg gctattcgtt 26880 tagttgctca aggtgcaccg gtagggttag ctgcaggagg ttttgaagta ggaggagttc 26940 gcataggatc ttctgaagaa aaccagaaat ttattgcaga tactgctgca ttagctctcc 27000 aggtagagcg tcttgataag gaagtagagg attcaactgg caatcttaat gcatggaaat 27060 cggcatacca agctgctggt gctgcagctg ctaaggctaa tccagaattc cagaagcaaa 27120 ttaacctaca gaaagatgca aatgaccctg atgcggttta tgacttcaac tctgcaactc 27180 tgaaaggact gacagaacag cagaaagcat atgatcaggc taagaaaact gctagtgatt 27240 tagctaacga tatccagaac atcgctcaga atactaatac tgcggctaaa actagtgcat 27300 ccttatcaga tacgattaag actattgagt ctctatccgc gggtactggt aaaaatgctg 27360 atgagtatgt taagagcctg aacttagggt ataatactct tagtgagatg aagactgctt 27420 cacaagctct tgctggttat gtaaaattaa ctggtaatga gactaagaat cagctagaag 27480 ttcagcagaa gattgccgaa gtgtatagcc agactaagga caaagagaaa gcacaggaag 27540 ctggtagacg tctggaaatc caacagttag aagaacagga agcagctctt aagcgtgtcc 27600 tagaaactaa caagggtaac aaagcaatcg aaggtgagat agctaagatt cagctagaac 27660 gtatcaagat cactaaccaa ggtatggaag ctcagaagaa ggttaaggac tataccgata 27720 agattcttgg tgttgatcgt gaaattgctc tcctgaataa ccgtactatg acagatactc 27780 aatatcgttt agctcagttg aaccttgaac tgactatcga gaaagaaaag tatgaatggt 27840 actccaaaca ggttgataag cataaggaag ctgaacaatc aagacgtgct caggcacaaa 27900 ttgaacgtga aatctggaaa ttccgtcagg atcagcaaag gggaatggct gctggcaggg 27960 aagaggagca acaaaggaaa tttaccgcaa atcctttaat gggagctgcg gaacgtatca 28020 aggagcaaat ccagctattc gaagacctta agcaaaaaac tttagggaat gctgctgctc 28080 agttagagta taataaaaag ctagcagaaa ctagagccca gctcgcagct ttaagagtac 28140 agcgcgatgc agaaatgcag tcttctgtag gtgcggctgt aggggctacg tatactccta 28200 ctactggatt aactggagaa gataaaaact tcgcagatat gcagaataga atggcgtcct 28260 atgaccaggc tatttctaag ctgtctgaac tgaactccga agcaaccgct gtagcacaaa 28320 gcatgggtaa cctaactaac gctatgatcc agttctcaca aggatctctg gataccactt 28380 ctctagtagc ggctggtatg caaactgtgt cttcaatgat tcagtatagt gttggtcaac 28440 aggtaagtgc tattgatgca gctattgcag cagaacagaa acgtgatggt aaatctgagc 28500 aatccaaagc taagatcaag aagttagagg ctgaaaagct caagatccag caagatgcag 28560 ctaagaagca aatcattatc cagacggcag tagcggtgat gcaagcagca acagctgttc 28620 cgtatccatt ctctattcct ctgatggttg cggctggttt agcaggtgct atggctcttg 28680 ctcaagcatc ctctgcctct ggtatgtctt ctattggaga ctctggtgct gagacagcgg 28740 gttacttaac tcttggagag cgccagaaga atgtagacgt ttctatgtcg gctaacgcag 28800 gtgaactttc ttatattcga ggagatcaag ggattggtaa tgctaactct ttcgtacctc 28860 gtgcagaagg tggtaatatg tatcctggag ttagttacca gatgggtgag catggtaccg 28920 aagtaattac tcctatggtt ccgatgaagg ctacccctac tgatgaactc aaaggatctt 28980 caaaaggcac atcgggtaga cctattgtgc tgaacatcag tacgatggat gcggctagct 29040 tccgagattt tgcttcgagc aatagtgctg ctttcaggga tgccgtggaa caggctctta 29100 atgagaatgg aaccaccctg aaatcacttg gtaattctta atactggagg aggactttat 29160 gtcctccttt tctttatgga aaaataaaaa tttcttgata aaattttcca atcctattat 29220 aatattgtta ttaagaggag aaattaacta tgagactacc agacccatac acgaacccag 29280 agctatcagg gttaggtttt gaaagcgtta acttagtaga caatgaccct atgattcgtg 29340 atgaattacc taatggtaaa gttagggagg ttaaggtgtc tgctcaatat tggggtatta 29400 atatctctta tccagaatta ttcccagatg aatttgctaa attagattca ttcatcctag 29460 attataaaag aacaggtagc tatattgatg tattattacc acaatatgaa gctttccgag 29520 ttcgtggtga tactagtgct gtaaatattc ctgctggaca aaaaggttca acagttatta 29580 tggacactaa gggtacactt cgtggactgc ctaaaccagg cgatctattt aaactgtcca 29640 accacccaaa agtgtacaaa atcacttcta tcaacacagc aggaaatact tggaatataa 29700 gtgtttaccc tgatctattc attactacta cgggtagtga gaaaccggta tttaatggta 29760 ttctatttag aaccaaatta atgaacggag acagcttcgg ctctacgtta aatagcaatg 29820 gtacatatac tggtatttca ctttccttga gggaaagtct atgaagaaaa ttcttgacag 29880 tgcaagagag tatttaagaa ctcataataa aataaaaact gcatgtctta ttactcttga 29940 attgcctggc tctactggat caagttctgc ctacatatat cttacagatt attttagaga 30000 cgtaatttat aatggtattc tttatacatc tggtaaagtt aagtctataa caacgcataa 30060 acaaaataga aaactttcca ttggtagcct ttcttttact atcacaggta ctgctgagga 30120 tgaagtatta aaactagtcc aaaatggtgt atccttccta gatagatcta tttcaattca 30180 tcaagcggtt attgatgaag agggtaacat tcttcctgta gatccagaca ctaatggtcc 30240 tttactttac ttccgtggta aaattactgg tggtggtatt aaggataaca ttggtacttc 30300 tggagttagt acatccgtta ttacatggaa ctgttcaaac caattctatg actttgatag 30360 agtaaatgga cgtttcacag atgatgctga ccatagaggg cttgagattg ttgccggcca 30420 attagttccg tctaatggtg ctaagcgacc tgaataccaa gaagactacg ggttcttcca 30480 cgctaacaag agtatctcta ttcttgctaa gtatcaggtt caggaagaac gttataagtt 30540 acaatctaag aaaaaattat ttggtctttc tagaagctat agtctaaaga aatattatga 30600 gactgtaact aaggaagtag atcttgattt taacttagct gctaaattcc tgccagtagt 30660 atatggagtt cagaaaattc ctggaatccc agtatttgca gatactgaac tgcataatcc 30720 taatatcgta tatgtagtat acgctttctg tgagggtgag attgatgggt tcctagattt 30780 ttactttggt gatgtaccaa tgatctgtat tgatcctaat gatagtaagt ctcgtacttg 30840 ctttggtact aagaaagtag ctggtgatac catgcaacgt attgcttctg gtcaaccaag 30900 ctctgaaccc tctgttcatg gacaggaata taagtataat gatggtaatg gcgatatccg 30960 tatctggact tatcatggta aggccgatca atctgctgct gatgttctag ttaacattgc 31020 aaaagagaaa ggtttctatt tacagaacat gaacgagaat gggccggaat actgggactc 31080 tcgttataaa ctgctagata ctgcttatgc tatcattcgc tttactatta atgaaaacag 31140 aacagaaatt ccagaagtta gcgctgaagt tcaaggtaag aagattaaga tctatcattc 31200 agatggtaga gtaactgctg ataagactag cttaaatggt atctggcaaa ctctggacta 31260 cctaacctct gatcgctatg gtgctaatat cactattgat cagttccctc tccagcaact 31320 tattcaagaa gcagctattc tggatataat tgatgaatcc tatcaggttt cttggcaacc 31380 ctactggaga tacgttggat ggactgatgc tgtaggagag aatagacaaa tagttcaaat 31440 gaacactatt ctcgatacct ctgagtcagt atttaaaaac gtgcagggat taatagagtc 31500 ttatgggggt gctatcaaca acttatctgg tcagtataga ataactgttg agaagttctc 31560 gaatacccca ttagagattg acttcttaga tacctatggt gatctggaat tgtcagatac 31620 tactggtaga aataaattca actcagtaca agcctctata ctagatcctg cattaagctg 31680 gaaaactaac tccattacat tctttaactc tatatttaag gaacaagata aagggttaga 31740 taaaaaactt caactttctt ttgcaaatat cactaactac tatactgcgc gtagctttgc 31800 agatagagaa ctgaaaaagt ctcgttactc acgaactctc acattctcat taccatatca 31860 tttcataggc attgagccta acgatccgat tgcttttaca tacggtcgtt atggttggga 31920 caagaaatac ttcctagttg atgaagttga aaactctaga gaaggtaaga ttaatattac 31980 actgcaagag tatggtgagg atgtgttcat taactccgag caggttgata atagtggtaa 32040 cgatatccct gatgttagta ataacgtact gcctcccaga gactttatgt atacaccaac 32100 accaggtgga caagtagggt ctattggtaa gaatggtgag ctatcttggc ttcctagctt 32160 aactaataac gttgtttatt actccatcgt tcattctgga catgctgatc cttatatcgt 32220 acagcaacta gaaactaatc caaatctacg aatgatccaa gaaattattg gagaacctgc 32280 tggtttagca gtctttgaga ttagagctgt agatattaat ggtagacgaa gttctcctgt 32340 aacattatca gtagaactta actctgctaa aaacttaagc gtcgttagta acttcagggt 32400 tactaataca gcttcaggag atgcttcgga gtttgtagga ccagacgtta aattggcctg 32460 ggatagaatt ccagaagaag atatcattga tggaatcttc tacactctcg aaatctacga 32520 taacctagat cgtttattaa gaagtgtacg aattgaagat cagtatgtct acgattatct 32580 actgatatac aataaggcag actatgctct tcataatgag gatgctctgg gtattaatag 32640 gaagttacgc ttccgtataa gagcagaagg tgataatggc gagcaatctg tggattgggc 32700 atctatttaa atgatttcga ataatgcacc agccaagatg gtcttaaata gtattatgac 32760 tgggtatacg atggcgtata tccagcattc tatctatacc gactacgatg ttatcggtag 32820 atcattctgg cttaaacttg gcgaagaagt ggatagaaga gatttcactg gaatcgacac 32880 tttctttgtt atgatcaata atttaactcc ctcaactacc tatcaggttc agggagcttt 32940 ttatgactcg attattgact cagaactttt aaatgcaaaa attggtatta acctctctaa 33000 tgaaactaac tttaaaacaa aagagaagcc aataattgtc gcagcaagat ctgagtcaga 33060 acctgtggat gttggggtgg gcgcaccgat agttgttgtg gaaacaactg gtgaagcaag 33120 ttactgtact attgagttaa aaagtacagc tactgaagac agtccttgga ctaaatatta 33180 tattggggct ttaggttcta ctattaaatt tggtggagtt cctatcggag actataagat 33240 cagaatatct ggtcaagtaa ctatgcctga tggtgttaca gttgattctt caggatacta 33300 cgagtttcct aatattctaa ctgtagctta taactttgtt ccacctactg cacctatcga 33360 tattgttttt aaagctgcac gaattgctga tggtaaagaa cgatatgatg ttagaattga 33420 gtgggattgg gaacgtggtg ctggtgctaa cgttcgtgaa tttttagtta cttatataaa 33480 ctccgaagaa tatgctaaaa ccggatgggc taaagctcaa aagataaacg tgggtgctgc 33540 tagagctgca acgattatat cattcccatg gaaagttgaa cataccttta aggtttcatc 33600 aattgcctgg ggaccaaata agcaagatat aacagaatca gctcctgtaa catttatatt 33660 gaatgaagat actcctctag acaacagctt tgtcaatgag actggtattg atgttaacta 33720 cgcctttatt aagggcagca tgaaagatgg ggaaatctgg agacagacat tcttaattga 33780 tgcagctact ggtgctatta acattggtct gctcgatgaa gaaggaaaaa cacctatttc 33840 tttcgaccct ataaaccgtg ttgttaacgt tgatggtaaa gtaattacta gagatattaa 33900 tgctgcgaac tttatcatga ctaacttgtc tggtaaagat aacccagcaa tttatactca 33960 gggtaaatcc tggggggata ataactctgg tatttggatg ggtatggata atacctctgc 34020 caaagctaaa ctagacattg gtaatgctac acaatggata cgttatgatg gtactactct 34080 gcgcatctct agtggtgtag taattggaac gccaaatggt gacgtagata ttggaactgg 34140 tttacagggt aagcaaacag tatttgttta taagttagca acatctttac cggccaaacc 34200 gctagaacaa gattatccac ctcctggttg gtcaaaaact ccacctaacc gtacagatat 34260 gacacaaaat atctatgcga ctacaggtac acttgatcca gttactaaca aacttcttga 34320 aggtactagc tggtctgatg tagttcagtg gagtggtact gaaggtacta taggacatga 34380 tggacagcgt ggtcctggga tgtactccat gggtattcct ggattaggcg gttgggatga 34440 tggacaagct aacgcattct tccaaaataa ctttggaaaa cccccggtta agtatgatgt 34500 tctaacacaa tttaacagta atgctccaca aacagcattt acccgtcaat ggagcggggc 34560 tggatggatt aaccctgcaa tggttcttca tggcaatatg attgttaatg gaactgttac 34620 tgcggataag attgtggcag gaaatgcctt cttatcacaa atcggtgtta atataatcta 34680 cgatagaaat gctgcgttat cagggaaccc tgaagcatac tacaagatga agatagacct 34740 aaacagtggg tatatccata taaggtaata taatgagtac agaaaaccga gttattgata 34800 ttgttgttga tgaaaaagta ccttatggtc ttatcatgca atttatggat gttgatgata 34860 gtgtatatcc accaacagaa gttcctgtta acttgacggg gtattcactc cgaggaacta 34920 ttaaatctag tttagatgag aacgccgagg ttctagctaa ctttaaaact cgtgttattg 34980 atgctgctca aggtgctgct gcaattagtc tttccgttgc agacgttact aatattggtg 35040 aaaaagcatc taaagaacgt gataagtata acccaagaca acgttttgct ggttattatg 35100 atatcctaat gactcgtgat gtaattggct ctgaagtcag ttctttccgt attatggaag 35160 gtaaagtata catcagcgat ggagtaactc aataatggca cttaaaacta aaattattgt 35220 acagcagatt ctgaacatag aagacactac aactactgct agtaagtatc ctaaatatac 35280 agtagtttta ggtaattcta ttagttctat tactgctggt gaattaactg ctgctgtaga 35340 agcctctgct gcttctgctg cggcagcaaa agattccgag gtagctgcaa agttatcgga 35400 aataaacgct agagattcag aaaacaaagc tgctatatat gctgactctt cagaagcttc 35460 tgcaactcaa tctgctgctt ctgctactga agcggagaga caagctggtt tatctaaaga 35520 tagtgctgat gcctctgcta cgtcagctga ggaatccaaa ggattccgtg attctgctga 35580 acttgctgca caaaatgctg aacagagtcg tctattagct gaacaagcta agaaggacgc 35640 tgaggctgct aagactgctg ctgctacttc tgagcaaaat gctgctacat ctgctactga 35700 gtctaccaat caggctattg ctgctgctgg ttctgctaca gaggcgggag aatacgctac 35760 tactgcaaaa gactccgaga tagctgctaa gacttcagaa cttaatgcta agaattctga 35820 gaatgaatct gctatttctg ctgaagcttc tgaagcttct gcttctcagt ctgctatttc 35880 tgcttctcaa tctgctgcat ccgctactaa agctgcagaa tcatcagatg cagcaaaaat 35940 tagtgaaact actgctatag aatcatcagc tgcagcaaaa actagtgaga ctaatgcaaa 36000 aactagtgag actaatgcaa aaactagtga gactaatgca gcagcatatg cagcagcagc 36060 aaaaactagt gagactaatg ctgctgattc cgctgcctct gcttctgact ccaaaggatt 36120 cagggatgaa gcagaagcat tcgctgcaca agcctccaca tcagcattag cagcaaaaaa 36180 ctcagaaact aatacaaaga ctagtgaaat taactcaaaa gctagtgaag acgctgctaa 36240 gctagctcag caaagtgcat caggtagtgc gaatacagct acgcaagcga tgaccacaac 36300 caaaggctac agagacgaag cagaggtatt taaaaatacc gccactactg ctgcaacgac 36360 agcaacagac aaggccctgg aggccgctgg tagcgctaca atagcaggag agaaagctac 36420 taatgccacc agtgcagcag atagagcgga gacagcggct gcatcagcag aacaggttat 36480 gcaagcgtcg ttaaagaagg atcagaacct taatgatctt gcaaacaaag accttgcaag 36540 ggaggcgcta aaagttgagg ctgttaattc tgtaaaagat caatatgctg gcgcttataa 36600 ttcttttcgt aatcctgcat ggacttatga attacggatc gctaacaatg gggaatggcg 36660 cgttgcgcgt aatgacaata acagcacatc tgcgctttct attggtgctg gcggtactgg 36720 tgctgaaaac gttgagggcg caaaaataaa ctttggcatt gatcgtttaa agcaaacaga 36780 aacagaaact atgatgtatg caccagggag taactcacct tatcgaatca caattagacc 36840 tgatgcggcg tggggtgttt ggactgatga aactggaaga tggattcctc tttcaattga 36900 tgctggcggc actggatcta atactgaagt tggagcaaga aagaacttaa atactcctgt 36960 tggtggtcaa gcaattatta ttcctaataa ttcaaacatt cttgggttta tgtcaacata 37020 cgcagaaagc ggttattact caagcggaga acttgttaca tatcaaccac ctgaagcctc 37080 tggttggtgg atgtatgaat tgcatgttca cggtaaaaat gcaaacggtc atgttgagta 37140 tggaaatatt gttgcaaccg caatgaacgg taataaatgg ggtatcattt gcagtgctgg 37200 ctcttggggt ggatggtaca ggatagcaag atctgatagg caattgatgt tattaagtcc 37260 ggatgctcaa tcggctcttg gtgattactc tatagcaatt ggggatcatg attcaggtct 37320 taaatgggat cgtgatggtc acataagcgc gtttgctgat tcagcgagaa tatttgcatg 37380 gactccatca ggaataaaca cttacagggt catatcatct tatgttgatg ataacgcaag 37440 gggtatgtat gtaaatggag ttaggcgcgg tgatccaaac gctcttattg ctggtcaagt 37500 tgagggtggt tcatttgcca actggcgtag tcgtgcgtct ggattgcttg ttgagcacac 37560 tggatttgat tctgcggttg ccatatttaa atctgtttat tgggggaaag attggatagc 37620 tggcatggat gttgttccgt ggacttctgg cggcgctgaa acacatcttt atgttaaggg 37680 cgctgaattt atttttgata gtgctggaaa tggtagcgca tcaaattggg ttagcaggtc 37740 tgacattaga ttaaaagcac atctaaaaga gatcgaaacg gcatccgaca aaattgatta 37800 tctaactggt tatacttact acaagcgcaa caatctaatt gaagatgaaa acagcgttta 37860 tagtattgag gctggattga tcgcacaaga tgttgaaagg gttttgccgg aagcggttca 37920 ttctttgaat aacgatggtc agctagaccc aaaaggcgag gcaatcaaag gcattaacta 37980 taatggtgtt gtcgcacttc ttgttaacgc attcaaagag caaaaagcaa agattgataa 38040 tcaacaggag gaaattaatg tattacgcaa tgagttatat gaactgaaaa accttgtaaa 38100 atcaatgctt aacggaaatg ctccaacaat tacagaacta ccgtaatata agtggagtgc 38160 aatctgtcac tgaaaaatat tgctgcgaaa caagcctgaa cattgattat cctagcaacg 38220 caacgcgtgt tctgctatgt taacagttcg ctgattctta ggctgttctt atgtacaata 38280 tgaaaacgca aaatgcaaaa tgcaaaatgc aagaggttcg ttttgcaccc tctcaaaaaa 38340 aaaaaaaaga ttaataaaat aaaggaattg gtaaaacaac ttattgctaa ataaaagaaa 38400 accccagtgg attgtccact ggggtttatt attatctact acctgcaata atacctaagt 38460 tatatactgc tagcatggct ttaagcatag gatcttgaga ttcagtgcaa agtgcgataa 38520 acccttcttc atcccactgt tctactacac cagatacatc aactctcagt actattttct 38580 caggttcaac gtatttaacg tcaatcttgt aaaagttaga ttgatctaca tctactccta 38640 ccatattagg gtcagtaaaa ttatttactt cctgagcttc caaagccgcc ttctccacga 38700 tcagtctcct ttagttcgtc aacgatttca aaatgatggg ttgagtagtg tggtagtacc 38760 actagctgac aaagtctctc gaaattctcc agagtttgca tttcagaacc gtagttaaac 38820 aggttcatct taatagtacc acggtagtct gagtcgatca ctcctgcggt atttgcgatc 38880 atcagtttac gcttacctaa agagctgcga ggaactacca aaccaaacca acctcgcgga 38940 atttccaccg cgacaccggt gtcgatcata agagatttgc ctggtgcaat agcacgtaaa 39000 tctgctgcaa ggttagtacc gaagaatgca cgcagatcca tacctgcggc atcatcagaa 39060 ccaatcttcg gcatgcaatc tggatgagtt agtttaattt taatcattgt cctgcgatct 39120 ccaaaatatc tttcgtaaac ttatctaata cgtcttgacc tacagcagca atagcatcta 39180 cacaataggt cggtaaatca accagaatca agtttcggaa aagtaactcc tccgatgcgt 39240 tcagattctg tatatatttc tgtttcccag gcagaggtag ctgatcaata atatccagaa 39300 cattaccaaa ttcacggata atattataac cacgttttgc accaatgcct tcaacgccac 39360 ggatgttatc accgagatct cccatgattg ctttcagaga gataaactga tctacatcgt 39420 caacattatg atgttcatac atatcacgaa gatggtactc acggcgtgta gtaaaggaga 39480 agcgagaaac ccgatcagtt aatagagtat cccagtcacc atcagtagag atcagccaaa 39540 cgtgatcata taaatgacca atgagcttaa caatataagc tgccatatca tctgcttcta 39600 caccacgaat agtgaaggtt gggaatgtgg tttcacacaa ctcgaaagca tcacgaaggt 39660 attcaaagaa ctgttcatcc agtgctttct cttcttctgt acgttgagca tacttttcat 39720 cacggttccc tttgtaatca ggaagatgct ctaaacggaa tacagacttt cctttatcac 39780 cgagaacgat tgtagttcta gctgaatagg attttgctag agattgaatt gtagaaacat 39840 aacttgatgc gaatggtttc ttactattat tgtgtttgaa gcggaagccc aagttagttc 39900 catcaacaat cattaggttg cgacgagaag ccagctcagc ttcttcctct tcaataaatt 39960 ttccccagga tttactcatt atttaattaa gtcctcaaca gatgcgtgat gtaaccacgg 40020 ttcgaataac ccgattacga tttccatgtc tttcttattt aacaccatat gggtacgact 40080 cattaagttg tccaccatcg ggtctgagct atccaaagca attaaccact gtcctctgtc 40140 tttcttgaat attaaagctg gtttggagtt catctgttca ccttcacggg aacactgctg 40200 ccaccacttc tctagagtgg attcaccaac attaaataaa ttacttgata tgctatcatc 40260 tttataccac ttaacttcga agcagtattt actaatgtgt ccgctttgtg gtggaaggta 40320 gatgtcaccc ttcagtccat ggctctgacc aaaagcacca gagcctggaa cacgttccca 40380 ctcaagacct gtacgttcac gcagtatatc tcttacctga tattcaccac gtttaccttt 40440 ctctctacta tctacggcca tgttttattc taagtaggaa aatccttctg catctttttt 40500 gacagtaatc ttatgggcta atggatgcgt atgcccatga gaaacaatga tagagttcag 40560 actatcttcc tcatttaata gttcaacaag agtatcaagt cctttcgtat caataaagct 40620 aataacttcg tcaaggaata gaagattaat attaactttg ctgatagatg ttaacagcat 40680 tcgaattgcc aacagagttg ctaggttaat tctactctgc tgaccagtag agcagttctc 40740 catactggta cggtttccat cattgaagat tactacttgt aatttagttt catcaagctc 40800 aaatccaagt gcgaacttac caccagtcat aatagaaaga tatttattaa tcaattcctc 40860 aaatactttc acactatgct ctagtttata tcctaccaga tttttcaatg cagctatcag 40920 aatatcaaga tcagtgacag cttctgatac tccatccagt ttggacgtta tctcagacat 40980 ttctgcctca gctttctcaa tctgttctag tttcgcttta tactttgcat tggctaattc 41040 gacatttgca ttatgttctt tggcaattgc aactttagaa cgtccatctg cgatttcctg 41100 ttctaattgt cggatttgca cctgtaggat ttgcacattg agttcttcaa aagaagcatc 41160 actcattgaa tttttgaatt cgtctctagc tgctactgcc ttatccaaag catccttagc 41220 tctagtatac gcagtgtact cgacttgttc tttcttcaac tgctctagcg tagcttcaag 41280 agattgcttc tctttaaata gaggatcata ctctgttcta gccatatcca ttgctttttg 41340 agcagcagtt gtatcaagat gagtgccaca agtaggacat tcagtatttg aagcctcttg 41400 cttgaacttc tgataacgtt tcttgacttc tcccgcacgt gaggtaacaa tcgttaggtc 41460 acgcgtaaca cttgagatct cttcagattg gtcagtgggc gcagggaaat ttttgaaagg 41520 ctcgaaagat tgttcggcaa cttgtacagc tttgtccaaa ttacggagtt tagtaatatt 41580 agcctcttgt gacttggcta atgccgcctt aattttcgat tcagtaagtt cttgtgccaa 41640 tggttcttca tcaaactctg gaacttcaac tggttcttgt aaacttccga gattgccttt 41700 tccattgagg atcttcgtaa ttacggccat ctggccttgc aagttactta acgtacttgc 41760 cacttcttta cgatcagcct taatagtttc tgacatctct ttatactgtt cctgattgaa 41820 cagattaaca agaaaagctt tacgtgtcgc atcagttgct ttgaggaagt ctagattgga 41880 accgacagat tgatagatca gtttagtgaa cgtttggaag tcaccgccca taatctcttc 41940 aatcatcttg tatgtttgag ttgctgtgtg tccactgata tcttctccat tcttaatcag 42000 tataacctta gcagttgatt taactacttt atgcaactca tactcatctt catctttcga 42060 gaagtaagcg tgcatatcgt actctttctt cggagaaccc caagagaata gggcgtcctt 42120 cttgatacca cgtgagttct tgttgtagaa caattcctcg ataacagtag caatagtgga 42180 cttccctaga ccgtttcctc cgattaactg agtaactgga ttcttatcga aatgaactac 42240 aatatcctta ccgtaagaca taacgttgct gaattttagt gtcttaatta taatctttga 42300 catatttagc agctctagct aagattctat caatgtcgtc ttgagacagc ttttcaactt 42360 cacggaagta aagttcaagt tcgcctaaca tatcaagatc aacaagattt aacttagcat 42420 ctttagtaac tcgatggtta atctttttat ctaagagatc ggaatcttta atagacttta 42480 actgaacgac gtcaccagtg acttcataaa ttacacggtc atagtcacta ggctccattt 42540 cctcaccaac tccgattgtt ttacgaatca gctgtggtaa gtcaccgagt tcaatccatt 42600 ctacttttaa tgtgtcggta tcaatgataa aacaaccatt tgtacctttt gtgcgttctc 42660 tgtggaacga tgtagttaat ggagatcctg gataaagaag tctagtagaa ccaacagttt 42720 gactattagt ataagaatgt aaatcgccag caattacagt gtcataacag ttatacctcg 42780 ttagatcaat ctctggtttt acatgtggag ggatttcacc acgaacatgc gtgaaacata 42840 gttttgactc agatggtttc cactttggtt tgtggatctc atcataaggg acaatatcaa 42900 attcaggaga acgatatggt ttagtaatga cttcccaatt tccgtttgtt actttattaa 42960 taactcctgc atagtgatac agacatgaaa tagttttagt taacatttca tggttccctg 43020 tgaagatctt gcctggatgg tcaagtcttg acatgaactg ttcaagaagt tctatttctt 43080 ccgacgatgg gtcggcaaca tcaagaatat caccaccagc aatatgcaga tcacagttat 43140 gattatggaa tatgtcattt aaccgttctc ctagcatcag gaagcgacgc ttctgccatt 43200 cctttggaac tttatcttgt cctaatttga tatgatgatc agcactaaat agtattctca 43260 taagttaaaa agaaagggag ccgtagctcc ccttggttta ttagtcgtcc agatcgctag 43320 cagcttcagc gtcgatgcct ttctgagaac cagcatctcc accagactta gcgtcaccgc 43380 cttcttgctt ctcacgacct tccatgaagg cgagaatagc ttctttctgc tcttcatagg 43440 acggaaccgg gtaggtttct tccagagaag gaactttttc gaacttgatg aactcaccag 43500 tatcatcaca catagcttca ccgaggatat caacatcagc ggcgtgcagt ttagactcag 43560 cactggtagg atctttcagt ttcatctgga actgcattgc tgcgatctgc tgtacatcgt 43620 actcagtatc gaagccttta cctttcttct cgatagaaat atcgatatcg aatggatctg 43680 ccagttcaag ctgcttcatg atagactgga tgcctttcag gatcgtagct ttaacttcca 43740 taactttcag tttgttatca gaacggtcga taacgaaagc gatgtagttt ttcttcggtt 43800 tcagcggaac acgattacca tctttgtcca gctctttctc gaagaaaccc atttcgtgaa 43860 tcgggtcagc tttaccgcga acgaagctct ctttagtacg gttgaaacgc aggcactcaa 43920 acggagctgc attaccatct ttgttagtca gccagtatac ataacgtgga agaacaccag 43980 aaacgatacg aacacgagtt acaccgttgt tgaatttcag gaagtcgatt ttatcgttag 44040 aaccgccagt agtttcgccc caagacttag ccatatttta tttcctcttt aaagattatc 44100 ttcgatttat tgattgcaat tagtgggtta gtgtcgatta cagaacgtgg tatccatatg 44160 gggacatatt gcatgtccaa actcggatcg tttgtaaact tatattcggc ataatttcgt 44220 agacttagaa ttcctagata ttctgccagt tgtctattag acaatttgta agggttatca 44280 acaatagttg attcattcaa gatgaatgaa gaaccgacca gtagttggtg agcatcaggc 44340 tctgcaagca ttctattaaa tagcttgaca atcaggtcgg agtttcctct agctaagagg 44400 taaattctct cgtaatcaaa gaatttcatt tttgtctctc atcaatttat gtatatatta 44460 tacattaaat cgggaggatt tgacaagtac aatttttatt tttctgcttc ggactttctt 44520 atcaatccga aactttctct ctcaaattta tgtatatatt atacaataat tatgagtagt 44580 tgccaaatgc aattttagct aattgtactg ctttctccgg agtcatagta atagttttcc 44640 atgcattatt gcgatacacg gccatacgtc cagaagcctg tcttaaacca gtacctcctt 44700 tcataattaa atctacaaca atcggatcta gctttccgtc aacaatacgt tgaacgcggc 44760 ctgctaactg ttctatgaga gattcattat taatgagact tcccattatt aaacaagaca 44820 gctcgttaag agaaatacct tctgagaaga tactctgagc tgctgcaagc acacagggtc 44880 cgccctccgc tacatctttc tggattctca atcggtcatc caaaggagtt gcacctataa 44940 tctcataggt tgtaacacca cgttgtgcga gagcttctaa tattgtttgg attagttccg 45000 tcctatcact tacaaagaga accttatgcc ccatagacac atataaatgt gctaggttta 45060 taatagtttc ccgatattca ggatgattat atacatcatt agcacgtagt gcccatggta 45120 cgttctggtt tcctgataac tcgacaggta cggagtacct atggattgtg ggtggaacag 45180 tgttattaac agggggacta aagatcttat atccaaagaa atctttgaac ataacttgta 45240 gcccatcttt acgttttaat gtacctgata gaccgatctt gtagcgagca catgagatct 45300 ctaggaaatt agtgaaagtt gtagctacac agtggtgaac ttcatcaact ataacagtac 45360 cgaatacctt agaaagatta ttcgcatgtt tgttcactgt ttgtatatta ctgaccacga 45420 ttggtggatc aatgttatac tttccagaac ctataatacc cggctcgaat ccaaaccatt 45480 tacgaacttc tgcagcccac atttcacgaa tggatgtgtt agtgcagatt accagagttt 45540 tctggccaaa cttatatgca agagctagag ctaggatagt tttaccaaat ccaggcttac 45600 cattaataat acaggtatca tcgcactctt catatatagg aagctgatct tcttcacgta 45660 gcttgaactg aggttctggt atttctaccg gagctaatgt gcgtttatca actaattcgt 45720 attttactcc cttagcatct agtaaatcca ggcgcgtaat gggaatccac ttaatctctt 45780 tagcaacaac accactattc ttatacataa tagggtattt actagtcatt gtctcgatgt 45840 gataagtggt ttgcttacta caataatccc agagttcatc atcgggcttg aaataggctt 45900 tattagatat aacaaccttc ataattttat tctaagtcta ggaatttcag gctcctcttg 45960 atggacttgg taaataacag ggctattatt taccagaata tagcttatat aagctggaac 46020 ataggatagc acgaatggat acgggacttt agccacatag cattgatact ttccattata 46080 aattcttgca gaatgcagaa ctttagacgt gataacatca tagaacgtag ttttcttcca 46140 gttaatgaga tttccatcag aatctatgaa ctggctacgc tttgacccaa ctaattggga 46200 caacattgat attctaccac gaataggata gagcttataa ggtaattctt tccgcttctc 46260 aaataaggct aacctacgtt gagagaatgt gccaggcaac tccctgttat ctagcacata 46320 tttattatat cttgttgtaa ttacggaata atcaccttcc tgctcaattg atacaaacgc 46380 ccgtaaagca tatacgggca atttgaaatc aagcacctaa aatccttttc acattatcca 46440 gatctttaca tacagcaata aacttatcat ccttgtactt gctgtggtct ggatgctctt 46500 tatccattgc agctaacttc ttatactcga aatctgcatc gagtaataca cccttaacat 46560 aacgagtata ctcatcatca tcaatacaag cgattgatgg gtgctgtttc ttcatcttac 46620 cacaagagta gtcacgagaa ccacctttct cagaatcaga atcaataccg attgggcagc 46680 cagggatact aataccacga tccttctgga tgttacgaat cagaatctcg ttatattgat 46740 caatcagatc ttcacgtaca atagcaacca cggagtcgtg aaccaacatg acgatcttca 46800 tctcttttcc tagacccagg gataggattt cttcatcagc atcaatagca cctaacagca 46860 ggctatcaga agatgcagac tgaatgattg cgttaaatcc agaacggatt tcttcaccct 46920 gaacaccacg atcttcggaa tggatgttgt gcagacgacg cttacgacca aagtgactat 46980 agatgaatcc aaagttcttg atctgatcgt ggcacttatc aatccaacgc ttaagctgag 47040 ggaactgacc gaagtaagtt tcgatataat ccttcgcatc tgcaacggta cattcaacaa 47100 acggttcgcc tgtcttagca gcctgttcca gaagagcttc gttaacagaa tgagctactt 47160 tagctgggcc agaaccatac agaataccga aggtaattgc cttagcagcc tgacgcagag 47220 ctgggaacag cttcttaaca tcacgtggtt cacaggtcag tttaaatacc atgtgtgcga 47280 tgtttgagtg gaagtcaggg tatttatcag gctcattctt catgttgata aatacttgtt 47340 gcatgtttct gtcaccagac agtacagcag cgtaataaac ttccgcagtt gttaagtccc 47400 atgcgattac acgatatcca gggggagcta ctacacaacc cttaataata gattcatcac 47460 gtggtaactg ttgcaggttt agcttaccag aagaactcag acgaccagaa gtagtcatgt 47520 gttcgtggaa accggtacga atgcaaccat ctgcatcgat gctcagaaga attttctcaa 47580 cataagtaga gatcagctta gtcagcttac gaatctctag cagagttttc gcaatcgggt 47640 gctgagtagc caattcattc agagcttctg cgtttgtaga atctgcacct gtatctgtca 47700 gtttaccagt tggagtcaga ccaacataat caaacagaag aacacgaagt tgcttaacag 47760 agttcgggtt aaatgcttcg ttctgatctt tctctagctg aacgacttct ggataagtat 47820 acagcttttc acgagccttg ttcaggttgt gagttaactg atactgagct tctttcaagc 47880 gatcaacaga aattggtaca ccacgatctt caacacgttg caggaatacg caaccaggca 47940 tcagaacatc atagtataga ctgcgaagtt tttcattctt ctcaattttc ggtaagaaga 48000 agttatgcag acgcagagta gcatcggtat cttttgcagc ataaggccac ataatatcaa 48060 atggaatcag atcatagctg aaatcttctt tcttgatctt atgtgctttg caataatcat 48120 ccttgaactt atccagttca aagtcgtaat cacccatgtc agtatacttc atagccagag 48180 atttcaagcc gtgagtacca cgacgttcat cgagaacata gtgttgtaac atggtatcat 48240 gaagtctacg ctctttatgt gatttctcga aggaaagtcc tagatgatag gagtaaaagt 48300 gcatatcgaa cttcaagtta tgaaaaacga tctgatgatt cttgctatcc agaattttct 48360 ggagataatg tacagctact tcggtgatgc tatcagagtc aatatagaca ccctgatact 48420 cttggtgaga catagaaaca ccaagaagat aaccatcacg agcatacaga gctgaggttt 48480 cggagtcgaa tgcgataggt ccgataacca tgttatacac catcttgata tattcttcag 48540 cttcatctgg atcagtaata gggcgataat caccagcttt tgcaatcttc tcacgaccat 48600 taataatgtc atgaatactt tctacagttg catcgaaaac tggtttcatt tcaggtttaa 48660 agtgtaactg agctggacta atacttgcaa tccaattggc atagccgcca tgctctacac 48720 gtttaccagt ataatcaccg atacctttct tacctgcgaa atacaggaaa ggttcagcac 48780 caactagtac aacaaaatcg taatcgttcg gatcgaacgg gttatctgga gttccgattg 48840 taatatgctt tttcagcaaa cggccagata acttctcgtt acacatatgg aatacatcaa 48900 cttcctcacc ataaagctgg aagtgtttat cataacgagt gttatttaga gctttatcaa 48960 ctactgcaat tttcaaattt agtctccttt tggtaagtaa gtataacttc agtgtttctt 49020 ctctaactta ccaatatatt ataccaaatc tttgagcgat tcggcaacta aaatttcaat 49080 acgttgagcc aacatatcga tctcttcttt atttaaatca cctgggtctt tgccttctgg 49140 aagaaggaag ttaccaacta caggtgttaa acgcgttttt gtacgaatta gcttcgctag 49200 tgcttgtgcg gctttattac cagaggcatc attatctaat aggataacta ctactcttac 49260 tccagcaata atgtaaggac tgaacttatc ggcaatgtta tcagatgtaa actgatgtgt 49320 accaaagcag caagaagcat aatccacacc gttatcttct agatttaaca tatcaaagat 49380 accttcaact agaacaagaa ctggtgtatt atatcgtaca ggaaaaatcg gtggtgaaac 49440 ttgttttggt tttactaagt atttaggagg ggcagaactg tttatagaac gtcccaggaa 49500 taggatattg cgtccaacag catctgttat tgggaataca attctgcctt cccagtctgc 49560 ttgatgttgg aaagcaaaat atttcttcaa ggtcttagaa ctgatacctc ggaaatcacc 49620 ttcgaaaagg taagcagatt caggaattgc aagattcgta gatccattcc taatctctga 49680 aatcttttta cgtacttgtg acaatcttgg tgactgtctg tactgagtct cattaaaata 49740 atggtaaatg ctcggtattc ctctaccgaa gccacaactc aagcagtgca taatacctga 49800 ttcaggatca atacgcaaac ttgggtgttt atcatcatga tctggattga gacaacagat 49860 gaggatgtcc ccacctgtgt ctttgtattc aatgcctttc agatcaagta gttctgttat 49920 tctactcata tatcgctggc ctgttcacct gttgaatttt cagctttgtc tttcttagca 49980 cgtttaggtt gagctggttt atccttttca ataggaataa cgaactcagc ttccatttgc 50040 gcaatatctt ccattgctag gttggtagtg ttatccattc gtagagtttc ccagttcatc 50100 ttaggcataa acttcacgct atcagaagaa cgagtcttaa cgaagtcaaa cataatagca 50160 ccttgaccat tatcagcttt tgcagcatta agattagcag ccatatctgc ggaatcaaga 50220 atccctttag acatacgcgt tctaccatct tggtcaatct ggtaaggagc aacaccagcc 50280 acattatgtt tctggcagat agatttgaaa gatgaactaa caaccatctg ttctttccag 50340 tcatacatgt caatggtctt tgaatctgga agtcttgttt ggttaatgta gtctagaaga 50400 gctacagtaa ccttatcacc atacttagca actagtttat ttaattctac gtcaaccgtc 50460 gtaatagaca gttcaggatc gtaaactatg atcataggag tgtgaagctc atagccttca 50520 aggagtttac tttccatatc atagaagtcg ctcattttag ccatagtgta ctgcttaacg 50580 aagttatcga aaagctcttc accaccatta aacatcctag ccctagttct ggctaatctc 50640 aacagagcag caccttcaag agtgttatta cgcattgcta atgcagatac cctagctaac 50700 atagccagat tacgtctaaa cacttcgtgc tctttcatct caattgagaa atatggagca 50760 atgtctccat tcaaatattg ctgaacctgt atgtttgaac agataatgga tttaccagta 50820 ccacgccaac caccaagcag taacgtttct gtgcgagcta aaccaatttg agcgtcgaac 50880 tcattacaaa taccaagagc gatcaagttc agtttggtat cttcttctct ctggaaaata 50940 cgcattgtgt ctgcgttgaa tacctttcca gtattcgtta ctttctcttc taattttaag 51000 tgaagggagg caactcggtt gagaatttct ccctgatcca gcattgttaa atcttgaagc 51060 acgtctgttt ctagaagttt caggaataga tcctgcgtat attcagcttc taggacttca 51120 agtgcctgtt ccatgctcac ttctggaatt tgagtgttag ctaagacgac aagagcttga 51180 gaaaggcggg cgttcctatt ggcctcaagc atcagtgcgt caatggacgg cattgcgtta 51240 tatttcttat aataattctg gacggcttgg taaattgagg agaaagcgtc attaaaatga 51300 tctttatgca gttttgagaa tgtttccaat gctatttgct tctgttcaga agcgagaagc 51360 atcttcaaca ctacagcttg cacgttaaac aaggtcattc tcctttgcac gctttcgtgc 51420 cttctaaatg caaaaagggg aaggagcata gccccctccc cttaggttta atttaccaga 51480 ttattcagca gccgcagctt ttgcatccag tttagcacgc ttagcggcac catcatagtc 51540 cttagcaacc agaccacgac gagacagcat agatttaaca ccgcgctcag acttaccagt 51600 tttctcagcg atctcagcaa cagtcatgtt agccagatcc agaccttcta acagatcttc 51660 acgagtttta gcacttgaag tttcctgtac cggcatagca gcgatacgac cttcacgaag 51720 cagactcaga gctttaccac ggatctgctt aatattgcga ccgaagtgag cagcgatagc 51780 ttcaatagta gcaccagcaa caacctgatt aacgaaatca gtttcttcgt caggagtgaa 51840 ggaacgaaca gcagcagctt tttcggttgg cttaacagaa gcagtcattt ccagactcag 51900 gatcttaccc tgtacctgct tagcaccgaa ctgaccacca gctacagcag cagcgatttc 51960 agcgtaagta tactgaccag catgagcatt caggaaatca accagttcag cttcctgctc 52020 aggagtccac ggggatttct gtacttcgtt agctttctgt acttcgaaac cttctttacg 52080 cagcttagat ccaacagagc gagcggtaac gtctttacca gtctctgtag ccagttcagc 52140 agcgatagca gctacttgtt cttgagagat tacagcaaca cccagagcag tggcttttgc 52200 tttcagagac tcggttacac cttctacgtt ccagttcagt ttagacatta ttatttttct 52260 ccaatagttc tttaatcgac aggatttcta tcccattcgt ttcggctttc ttataagatg 52320 aggaagaacg ttttgattca tcctcacaga ttaggtattt gacatctttg gtaacggact 52380 tcttaaccgt atatcctaga ctttctaaat agtttgttgc atccgttcga tttgcaaaat 52440 cttgcaaaga tccggtaata cagaccgtga ttccatttgg ttgggcaact aaatcatccg 52500 taatgatgat atcagcttta gtgcctcgaa taccagttga gaatttccac ggtaattcaa 52560 taacatcctt gccttgtggg gaatttagcc aggttttgta attttctcca gccttgccgt 52620 cagccttcac atcgtggaag ctagtgcaat tttgggacag tttctttgct gcaacctctc 52680 caataagagg aattcctaaa gaaccgagaa ctgaaccaaa gtcaatgtct ccgcgaacct 52740 tagtatttaa ctcgctaatc aacttagcgg caaccttgct acccacagct ccgaccaaat 52800 cttcttcggt caggtaaaac agttctgaga tcttagtcag ctccagcttc tcaatagttt 52860 tcgggccaaa gccctttaac ttcatttttg tgcagaagtt ctcgattaac ttacttgatt 52920 gcgctggaca gttggactta ttcctacaga ataattgccc gttgacaaga tctagcttag 52980 aaccacaaga gggacattgt gttggaattt cgattttcat caaatttctt ccttatcaat 53040 ttatataaat attatagcaa gtatttaagc atttagcaac tacaatttta actaactttg 53100 cttaccctcg ccataacttt ctatccccca actgaatgat aatagtatat acctaagcgg 53160 cgaaaatgtc aataaccact ttataattcc gacatggtaa acgggtagca aagggttacg 53220 aattagtcgt atactcgctc tactatacaa gggattacac caccagcacg aatcactcga 53280 atctggcaac cgatctctaa atcgagagcg ttaatatagt caacgttatt aagagttgct 53340 ttaacaattg tggcatcatc aataatcacc ggctcgaaat aaccaactgg agttacttta 53400 ccagaagccc ctacttgcca ttcaacttta acaagtgtgg taatttcgcc ttcttcatcc 53460 tctttaatag caaatgcacc acgaggaaat ttattagtcc aaccttcacg gaagaattta 53520 ttgttgtcgt taatacgaac aactttaccg tccgtcggaa tccatttaaa aaaggaacga 53580 acattaacaa ctgtcaagaa gttctcattc tctagccaaa gcatatcttt taaataggct 53640 tctgtaatac cgactgtttc cgctgaacac tggataccat atgcaacgaa gattaaacca 53700 ccttcaccaa tacgttgcat gaaatcatca ctatctttca ggttaatagc acccgaagcg 53760 tagttacgtt tgttttctac ttctttagta atgagaactt caccagtgat ttgagttggt 53820 actttttggg aaatagtttt gggaatgttc agcattttaa cattatgcgt tacatcattc 53880 cctagaatac cgttaccacg agttaacgct tgaacaaatt caccattaat atataataga 53940 gaaatcgcac aaccatctaa cttatcagtt tcaacctgac ctaatgggtt aaatggagct 54000 ttatcaccac gatcgtaata gactttctgt aaagaataca tacgatacaa atgagggata 54060 tcacctttcg gtccgatttc ttcctctagt gggaatcttt taatcagacg atcatattct 54120 tcatcggaaa taagactcat tccctggtag tacgcttctt gacaacgttt aataaagtct 54180 tttacattat tcatcatctg cgatttccat tttgagttct ttactatact gcgcagccat 54240 accagctaaa tcagcagctt ctttcaacca cgcatctttg tacgctttct ggtttggagt 54300 gccgatacta tcctggtaat tgattcttgc tatggagagc aaattaagtt ttgcttgaat 54360 ctttggaagt gttattgtca ttgccataat tgatcctctc atcaatttat ataaatatta 54420 tatatgaaaa ctgagtcgaa agcaagtaca tttataaaga aaaagccaag aactagacat 54480 tcttggcttt gagttcttca gctcgtttct gtacttcatg caagacctct gattcactga 54540 ggatttgcgt aaaagcgtag aatagctgtg atgtagtttc tagggtataa ggaaaagaga 54600 agccggattt cgttggaaac cactctccag taatatcttg gagccagtaa cgaattccca 54660 tgtatagatt cccacgaaat tcagatacgg ttagtcttac ttgttccccc tccttctccc 54720 aaagcatgat ggactggtcg tcaacgtgtc cttcatagtt ttgatttacc tgatcgctca 54780 tttacatatc ccaaatacac aaaaagcccc atataggggc taattggttt cttatcggcc 54840 tactggagaa gcacgatcta actctgactg gaggctcgtc acacggcgaa ctttcgcaac 54900 aggaatgaaa cggaagctat cgttagtacg agagaaaacg aggatttcat cttctttcgg 54960 cttacggatg cgctcacgtt taatacgctc agccaggtat ttatcctgag ctggatcaaa 55020 ttccatcgta ccctgaaggt acgtggttcc tttctgacga agtttttcgt aatgaagata 55080 aaactcacca aatttttcgc attgagcaat aatttcagct ttattcataa ctgtattcct 55140 tgtgttggtg gatttatcta agaattactt agtgatagct cggatagctt ctgccagatg 55200 agcagctgct ttaccggtca gcttgtcgat gattgcgtcg tccaggaagt ccggggctaa 55260 gccagcatca gagaatgcag agcgaagatc ggagtgagcc tgagctttgg aagtacgaga 55320 accaccagaa gctttttctc cagtagatgc gcttgattta gaagtagagc cagcagcttt 55380 cttgatatac agacctgctt tggtcagctt catacggaaa ccgttcggag ttacaccatt 55440 ttcctgagca atttcgctaa caatttccat gctaacgccc ggacgctcat cttctgggaa 55500 ttgctccata cgagcaacgt agtcggaaga cattttttcg aacagttcat cagtccactg 55560 agttggagta gtcatatttt tcgatttcct taattaagaa ttaaacagaa tagttctttc 55620 aaactatgag aatattatat caagaattac aggattaagc aactgaaatt tttcagtaac 55680 tgctacacat ctagttcaac gatctcccaa tcgtcggcca gggcttcttc tagggttagt 55740 tcacacactt catcacttgg gaataatgat gtatcaaaag gcatccacac gtactggcgt 55800 acggtattgc tatctacaaa gaatttcagg attactgtgt aatctttatt attttcccaa 55860 ctagttcgct ggagatattt tacgtctcca gcttttaaca gatcagttag tttactcacg 55920 acgttttcct ttctctttat ctagtttcag ggcagcttta acagcttcat taaccaacgt 55980 gataacttct tcacgactcc acttaaagcc cagagcctta acatcaacac ccagcttttc 56040 cagatgttta acagaagcta attcgtagtg catatagtga acgttctgtt gtttaccttc 56100 tgataacaac caaacacgat agcaaccaac tggattagtc attgctttct tgatttcacc 56160 aatacactgg tagccaggaa cccaaacaag ttctccaact tcaaattctt ctgccattgc 56220 atcatcagga atcagcggtg gattcaatgg atcaacaata tcgtttaaac gaagcagagc 56280 accataacgt tccagaacag atttaatcat cgctacagaa cgataattac gatctgcgat 56340 ttcttcaaaa gaatcaccag aaagatactg ttcaattacg tttgcaagct caaccccctc 56400 aatcagtgtt ccgcgcttct tctttttcat ttccgcaact tgaacctgac gatctctcca 56460 ttcttcgatc attcgttcca tagttgggtt agaagaaaca ccgagcatct cacatgctgc 56520 tttcttagta ccaccattct ccagatgttc aataaccttt ttaaaggttt catcagggat 56580 ttcatggata agtttctttc tacgagaacc agccatattt aaactcctct ctcaaattta 56640 tgaatctatt atacagaaaa tttttcctga aagcaaataa aatttttatg attctttgag 56700 caaagaagca agttcagcat agactttatc taactcaaag ccaatgcgaa gagacacacg 56760 agtcttccgt tgtttaagta accatatgga gacattaggg atgccagcga gacgaggtgg 56820 tacaacccta catagtttct ccgcagtatc gtaaacagaa gttttacgtt cttttactgg 56880 aaacttcctt gtttcccact ttttattctt cactgtcatt tctagctccc aattcagcaa 56940 gagcttcctc gtgcatctga cgttcttcgt ctgtcataaa cttaactgga atcggcatac 57000 attcgatctt acagtaagtt ctgtaccagt ccacaatctt ttccgtatcc agatctttac 57060 cgatacccat catgccaaga tacatacgag catattttgg gttagcgctc tgacctgtct 57120 taaggaagaa atccttcttc ttattcttaa gagcttcgat aaacggtttg attgttacgt 57180 ttgaacactt tttaatatct tcccagaaca tgtcgtgcat cttgtggaaa aatgcagctc 57240 gatcattcgg tttatccttg atatactgtt caacatgttc cactgttaca tctttaatag 57300 aatccagatg ataatagcgg accagaagta acttggcttc ataggcatct gcatgacgtg 57360 gtgcacaata ttcaggagtt gagtgcaaaa tagctttgat tcgctcttct gtatactctt 57420 ttcctgcacc ttcgatagtt ccccagcttt cactgaaaat atcaatagtt gcaccttgat 57480 cgagaagata ataccgcaaa gtactggtat agtttctaga ttccagctca cgacgacgtg 57540 cgtacagttg ttgggctaac tcaatccaac cttcgtcaat attatattgc tcggaacccg 57600 cagaaaatcc actcgtagat ttatcaataa aataataaat gttctgagca ccacggtcgc 57660 gcctgattgc ttgaaaacgc atgtttggcg cttggtttga ggttctagtg attacgaata 57720 cgttatcaaa atagttaaag tcaacaccac tcgttacaga tggactacat aataaacaat 57780 caatctgttg atcaattagc tcattagttg tataatccag aatacgtcgg atatctacat 57840 cagacgtaga gtttgagtga atttctttaa ctaacgcacc agtattacga cgtaatgcca 57900 ttcccttctc attcagttca tccgggccac agtcagatac taggattgac ttctcaccca 57960 tctctagaga tgtttgaagt gcaacccaaa tactggattc gtcagggaat tcataagcgt 58020 gagctttcgc cagcatttta cggtgatgat tatagaatgc aactggttta tcaaaatcaa 58080 tcaaagaacc ataggcttca atcgtttctg cactgatatc accatcagat aagatcaccg 58140 ttttagcagt aataagaata tcgcgaagaa cctgtataca ttcacgacgg tttttaacaa 58200 ccggagcaaa taacagatca ttcattactg cgtcacattc atcaataaag atcgtatcaa 58260 tctgacccac aaagctcttg aacttatgga tagagtgaat agttgaagac atacgatcaa 58320 tggcaccacg tttgaagttc agcatatcaa ctgacttatc atactgacca gcaccgaatt 58380 tcttcgcgtt tgaagataca agtgccctag tatttgtgat tgcaataaag tttcctggaa 58440 gaacacctgc atctagccac ttagttactg cggtagtttt acccgtacca agacttgcct 58500 taacaaatgt taaatgtcct tttggtggta caatgttaat tttcaggtat ggaacttctg 58560 gtggagaatc tgtgtctaga cgttttaatg gtacaccttt taagtttggt ggaatctcac 58620 gtttggagtt atttacaaag gctttgagag cctgcttacg accgttatta aagtaatctt 58680 gaatattacg actattatct ttagttgcaa tatattcaga taaagctggt ttaatctcac 58740 gttctaacca tgtaaagtca acaccatctt ctaacgctct gtggtaaagt tttggaataa 58800 tacgcaggta caccccatcc tcagcttctt ctagttcgct gatggtttct tccactttat 58860 cagatgcagc cttcttgcct ttaatttggt caagtaagga gtagaattct tctttaaact 58920 cacctctagt tgtttcaaag tcaactaggt tattgggtag attcactttc gaacccttaa 58980 catatacaag acgtggttta ttctccgctt taaacggatc gactatacca tcatcaaata 59040 agggatctgc aaaatagtga agctgaacag aagaataata agctaagtca gcaatatcaa 59100 agccatactt ttgcttacta ctgtcattga tagatgtaaa taagaacttt aactgtccct 59160 gcgttacctt gacattagat tctagcatta agtgcattcg gatacctggt tttaatccag 59220 ccgaagacga tgcatgggca ataaaccctg catcaagagg aaacatatcc tcactaatac 59280 tattcaacat cttaataata tgacgtgcca tgccaacaag attaaacttg tcatagccac 59340 cagtatctac aataccatcc acgtccatag caataatatg actaggattg gatacgttaa 59400 agtttcctct ggtacgacgc acattatctt ttggagctag acacttaccc cgaatagcta 59460 caatatgcgg gtctttggta agacgacgca tgataggtaa catttcagca aggttttgcg 59520 ggtcaagttc ttcaagtaca tcaaatttga agggcatgga agctggttta ccttctggat 59580 gctctttaga gaatcgcttt gcaaaggtgt aatcttccac tttgatctct ttccagatgc 59640 cagtggcttt atctctggaa aatcctgcgt gaccttgtag gattgagaac acaacaatac 59700 tcctatgttg gttattcgga tatctttctt taggactatt caaaagaata gaacaactat 59760 tactaccatt tacacaaaca ttaatcacca tcaagcagta tgaataagtt atcatacctc 59820 agtgctgcgc tctcttaact cttatccaga tcagaaccta ggtcattggc cgctaagccg 59880 attctgattg ccctcactat cctattggta ggtggctact ggacgtgtca ccattcgctg 59940 ctcttttcgt tcgcaccccc atataaagct ctgcctatat aacgagctaa atatgcacgg 60000 tttggattta cctttccgaa ttcagggtac gcagatcact taattgataa ttaactaatg 60060 cagtataaac actagtaata gttaaaacaa cccttaatgg ggcagcctaa ctgttccgta 60120 ttgcggattg taaacaagaa aaattagcaa aatcaatttt tactaatgtc tctcaattca 60180 atataaatat tatatcaaga ttttgagcat cctgcaagta aaatttttcc gaaggtgcta 60240 tcagtgtctt tgagcacaga gaaaaccgag ccaaaatgac tcggtaatca ggattgggct 60300 aatgaaaatc aatcatcatt taggcctggg acatagatcc caatgcgatt taattgacct 60360 ttagcttctt cgagaatatc ttcggtgtac tcccaaatat catcttctac ttgcaaaaga 60420 taaggggatt caccagtttc ggggtcacgt gtaatggcta agccacgtgg atggaatata 60480 gagaagttga gggcaacaat caagcccatg ctggacaatt cattccagtc agcttcccaa 60540 ccatcatctt catcttcatc cacaaagact cctacactag ggtctgttac agaagtaaac 60600 ggaaatgtta cactaacctt gtcaaccccc aaccaacgtg caaggagccc ctcagcacgc 60660 tctaccaaac taggatctat ctgacgccaa aatacttgtt ccattctgcc tccttaagca 60720 gttaaacctg ggatcatata cccgaaacga cgaagtttct gaatcccctc atttacttcc 60780 ccgggcgagt atgaccagcc ctcacgttca tttacctgga aagcaggagc ttcaccagtg 60840 cgataattta caataagacg gtgatttgca ggcataaaca gcttatggtt caaggcaaaa 60900 accagaccca tttcatcaag ttctttccag tctacaatct cttctactcc ctcgcctaaa 60960 tccacactaa ctccaataca cggagcttcc tctagcgggc ctttaaaaga caggaagata 61020 tcgttattct cacaccaaat cttaaggaga ttgatggttt cattatacat accattaggg 61080 agagagcgcc agaagttgag aagcatagga tttacctctt tcatgaaaat attattgaac 61140 atgcttatac cttaagtttc tgaagagcct tacacatttc aagatctgga ctaaactcca 61200 gcatatagct gatagcgttt aaatcttgaa ggtttattcc agtcctgccg tttacaataa 61260 cagtaggaaa ttgatacatc atagcaccag catcatctag gacacaccaa tgcttgagct 61320 tatatttttc gacaaaacgt aaaacagaat tacctcgaga taaaccacct ccagtaaaat 61380 ctgttgtacc aagaaaacga tcgatcaaac cgagtccggt cataatctga acgttctcca 61440 tttcatttcg tacagaaaac catgaagata ccccaacaat cattattggg gatggctcaa 61500 gaaagttctg aaaagcctgg agtaatggct tgtaaaccca atcaccccca aagaatagct 61560 tctcactatc tggagcatga tggtgagaga tgctcgaatt gagcacccca tcaatatcca 61620 ggaaaatcac aggagtatta cttatttcca ccagtagcat cccacgtcca gactttaact 61680 tcatcagtaa aaggaactgg aaccttcaat tcacctttac cccacataac agataggaac 61740 agagaataaa tggtaatcat accgattagg ttgacaaacc atactagacc tttccagata 61800 cccttaaaaa tcttcttcac catattctcc ctccttaata aggaactctt tccattctgc 61860 tgtatcctca tagataacgc agaaatcatc actcttccat gagaagtcat cagtagcaaa 61920 ttcgaaacgg tagcaccaac taccgtctgg ccatactaaa agatcacaca tgcttttctg 61980 ggacataaat atttccctcc ataaaagctt caacttgatc aataaagtgt ttattaccaa 62040 aaacttcggc agctccagca cgccatacag taacaccacg tttctcatac tcaagctgtt 62100 cgtcataacg aacttgtggg ttaacaacca tcatcttagt tccacgacat acagcaggaa 62160 atagctccca gttgaagtcg attacttggt tgctacaacc tactacaata accatatcat 62220 tgctagtaag tgtatcaaac aggttaatct gacccatata ccacggagcc gtctcaccaa 62280 agaatgttac gtttggtttt acccattcgt aatcctcagg ggcaatcgca gtatacccaa 62340 catcttcgat gatttggttg cgaccctgac gatagatcac ctctttcaag tacccgtgag 62400 cgtgaatgat ttcatcacga gggataccag cacgttccag aaggtcatca acgttagtag 62460 tgaagttctt cacgctatcc tcaccataac gctggaacca ttctgcaatt cgcagatggg 62520 caatgttagg ctcaacagtc tgtaattcct gacgacgcat gttgtagaac tcatgagtct 62580 tgagatacag atcgttacca tcttcatcaa tgccagggag acgagcactg aagtagttag 62640 gatcatcttc accacagaag cgacagaagc cagcttcaaa gttaggcaag ctacatactt 62700 cctctagatc gtactcatcc cacaaggatt tcccactagc agtatcagta cggaaagcac 62760 gaacaccact atctacactc aaacctgcac cactaacaac gattagtcta cgcattatac 62820 gattccttgc ttgaatcctt cgatgatatt ttggagcaga attgcttctt ctgggcgaac 62880 cttctcttgc ttaataagca tatccaaacc agcaatacag gtagctactc cagcattata 62940 cccagcattc cactgctcca taagcatatt ttctaaatta ctttttagat tctgattcaa 63000 gaagtccata aagtttttcc tccaattcca taatacgatt ttcataagaa gcacgcagct 63060 tctcgatgga caaagcacga actgtaactt catctgaatt gatcgctagc tgagctagat 63120 cttctgtttc catacgtccg ttgtaaatta agcgatattt aagatgtttt acgtttttca 63180 ttcttattcc ttagttctgt actgtaaatt cattcttatc acagtgagca taataatacc 63240 catacatacg ataaattttc acagccttag tagacccctt cgattcttca taatcaatcc 63300 agagccactt gtctttacct acataatgta cccgaaactt cttaactttc ttatcattcc 63360 atgttgcaac aacacgacga taatcaaatc cgcccaacca atctagagcc ttacgttctt 63420 cactaggctc tccaagatgg gaaacatcta ctttcggaag caattcgctc atagctttct 63480 cctctcaaat ttataaatca attatacata agttttagct cgaaagcaaa taaaataaaa 63540 gccaggtcga ctagcaacct ggctgattca ttatttagcg cgaatgtctt gatgtttcac 63600 tcgacccata acctcatgtt gtttacctgg gttaaacgga cgagcacctg gatttccaag 63660 atatccacaa actcgacgag ttacctcaag agtctcagga tcatgattgc cacaaattgg 63720 gcatttgaaa ccatcctcag aagctatagt ttcacccaag aacccacatt ccccacaaga 63780 gtctactgga gtattaatac cgaagtaatg aactttactt gcagcatagt taataaccca 63840 ttccagtgca tcagggaatc gtttcatatc cggtaattcc acataggaaa tgcacccacc 63900 agaagcaata ggagtaaagt tagactcata atcaaacttc acatttggag caaccttacg 63960 ttctacatca aggtgatggg aattagtgta atatccttta gccagaatat cttcatgctc 64020 tgggaaatac tcacggtcta ggcgacagaa gcggtcacat agagattcag atggtgtagc 64080 atacagacta taccctaatt gagtctcttc cttcttctga tcagcacgat cacgcatgta 64140 ttgtagcaca cgttgaacga acttgataca agactcagac attgtatcta catctttacc 64200 aaacatgagc tgcaacattt cgtggcaacc aatatatcct agagatacag aagcacgctt 64260 gtagaaatga tcaaatacat attcatctgg ccccagacgt aatccaaagg ctcctgacat 64320 atagaggatg ggcgcagctt tagcttgaat gtattttaag cgatcaaccc tccactccaa 64380 tgcttttaac gcggtatcca cacgcagctc aagcagatcg aagaatagat caatatttcc 64440 ttccgcttcg atagcgattc gaggaaggtt tacggaaaca acgcctaagt tgttacgacc 64500 agaagtttca ccatcttccg cagcagccag gaacgaacga cagcccatag agactttgta 64560 gtcccccgta acttctacaa ccttatcata gctgatatag tccggataca tacgttcagc 64620 agtacacttc atagctaact tcttgatatc atagttaaca tcgccaggtt tcatgtttac 64680 gccgtcacgt agaacaaata ccagtttagg gaagatagca gtacgtttat tgatacccaa 64740 accatccata cgcacttcta agattgcttt ctgtacaaga cgtgcttccc aagaagttcc 64800 taaaccgaaa ccaaaggtaa taaatggact ctgaccatta gagttgaaca tcgtattgat 64860 ttcgtactct agaccctggc aggcatcgta aacttctttc tcagtcattt ccgtagccat 64920 tacagatgct ttggcttcat ccttagtcca acgttttgca aacgcatgat tcttatcata 64980 agacttacga acataaggag ccaatacttc atctaatcgg tctacagaag taccaccata 65040 ttggtgtgag ctaacctgag tgataatctg agctgtaata gcagccgcag tagtgataga 65100 gtttggcgta ctgatttcag cattaccaac cttcatacca ttcttcaaca tatcttccaa 65160 agcaaccagg caacagttag tcatacctaa cgcagcgtaa tcagcatcat ggaagtgaat 65220 atcaccaatc atgtgagcat ttcgaatctg gatcggcatt tcttccagaa tcagatattt 65280 actcaattcc cctgcaagca tatccctttg tgtagggaaa cgttctgagg gcttattggc 65340 attattaaac agcagctctt catctgctga accttcgata atttctttgc agtttcgaat 65400 caaggactgc attcctttat actttagttc ttcattgcga ttcattatac cctccttctc 65460 taactaagac tactattata actcattcga aggcggatgt caacaatatt ttactataac 65520 attctgtaaa ttgatctgag tcaaaaaatt ttatttgcca acctctacta aaaatagtat 65580 aatcacgcac ccgtgtacgc gatataaaat gattatctag taccatacat ctcccaataa 65640 aagcaagcaa attttttatt taaatgcaat atcataggct tatttaaaat tgtagttgct 65700 ttctcccgca tatattggta taatatattt gtaagttgat aaacaagatc tttttgttga 65760 tcctctgtta gcgaagtaca ctataggagt gttatttatg ggaaaagcac gtcaaaaaag 65820 agagaaccgc aatggttcaa gaaagcgtgg caacaagtac gagagtaacg taattcaagc 65880 agatttctct ggtgattacg ccaatccagt tgctaaatcc ctagttggta aaaaccgtga 65940 gcaaaaatca tatatcaata tgatcaagaa caacacagtg actgtgggta ttggtgagcc 66000 aggtactggt aaaaccttta ttccatccgt tcttgcagct caggaactcg tagacattca 66060 ctcagatatt gagcaagtgt tcctcgtgcg tccaaacgaa cctctaggta aatctcttgg 66120 tatgctccca ggtgatctag cagaaaagct agagccttgg ttggaaccaa tagccgatgg 66180 catgaaatgg gcaatcggtg atcacgccta caaaggatat gtcgaacgtc agaaaattaa 66240 attcctggct atcgaacatg cccgtggccg aactttcaac aactcctatg taattgtcga 66300 tgaagctcag aatatctccg ttgaagcaat gatctgtctc ttgactcgtg tcggacaaga 66360 ttgtcgtcta attatctgtg gagatatagc tcagaaagac atcaagggtg attcaggtct 66420 agcacttctt atggaagtct atgagaagta cgaaaactct cctttctcta tgatcgaact 66480 gatcgacaac gttcgctcac cagagtctaa agcattctac gatatcttta aggatatggg 66540 gaaggtgtaa tatgggaaac gtcgtacatc tgcaccgcaa aactaagatc caccgtactt 66600 cattgagtgc tgctaatatg attacacgta aggaaggaga agaaagtcct aagactaccc 66660 tagcgtggaa aattgtagcc tctaatccaa atcggccttt caactataat gaactgagta 66720 cttctataga cattcttatg gctaaggaaa tacgacagaa actaactgat atgaccgata 66780 aacttaaatg gtaagacaga cgagcctggg tatgaaaata tccgggctct ttttatctgt 66840 aaaattgtac ttgcaaaatg acccaaaaca aagtagaata tttcctaaat cctgataact 66900 acaaaaagga aagccaaatg agccatcgca tcgaaaaagt aattaaacgt gacggtactg 66960 ttcaggactt tgctcctgaa aaactcaatg attgggctgc atgggcatgt aaagatactg 67020 acgtaagttg gtctgccgta gttatggctg ccctgaaaaa tctacccaaa ggtgttgtag 67080 actccgatac actccaagat gtgttaatta aagccgctga aggtcttatc aaagacaacc 67140 cagcatatga tactgtagca aaggaattac gtcttgcgca gatgcgtaaa cgcctatatg 67200 attcctttga acctccttct ctgcgtttct tccacgatca tatggttcgt gcaggcgctt 67260 gggaagacat gagtgcatgg attagcgatg atcagtttga cgctctgaac caagttattg 67320 atcatgatcg tgatcgttta ttcaccagtg gtggactgaa acagttcttc gacaagtatt 67380 ctcgtcgtaa tattgcaact ggtgaaattt atgaaacccc gcagtttgcc tatatgggta 67440 tggcaatggc gatgttatct caacctaact ggacaattct cgatgcaatc gacctctaca 67500 acgcaatgtc actccataaa atcaacgtgc cgactccacc tctcgtgggt cttaggagca 67560 gcgaccgcgg ttttgcttct tgttgtcttg ttgatagtac tgataccctc gattcaatcg 67620 acacagccga gcatatcgtc ttcaaaatgg tcgcagccag agcgggaatc gggtatcatc 67680 ttgaaagccg atcaattgct gatccggtgc gaaatggggc attcccgcat tccggaaaac 67740 tgccatatta tcgacacatt gaccgttcag taaaagctaa cactcagcaa acccgtggcg 67800 gttctgctac tgtgtcttat ccatacttcg atcctgaaat cattcaattg atgcaggtta 67860 aacaacaacg tgctacagac gagaataaaa tcgataaaat ggattactct ctgagcttta 67920 acaaccttct gttaaaacgt tacctgaaaa acgaagatat tacgctaatg tcctacttct 67980 acgcaccaga agttcatgaa gcgttttatg atgaagatga ggctaagttt gaagaaatct 68040 acgtggcagc ggagaaacgt gtggcatctc ttacaaagat cgaccacgaa ggaaaaacaa 68100 ttccagcagc tcctagaatc tctgcaaaag aaatcctaga tacttggtta cgtatccgta 68160 tggaaacagg gcgtatgtat gctcatcaca ttggtgaatc taaccgtcat ggtaacttcc 68220 ttgacccgat tcgtatgacc aacctttgtg ttgaaattac tcagccaacc cgtccattcc 68280 atcacattac ggaactgtat aagacccaag atcagttaga cgatatgcgt ccagaggaca 68340 ttggtgaagt atctctgtgt aacttaggtg gtgttgtact aggtcgcatg gaatctctgg 68400 ctgaatggga gaaaacttgc tacatccttc tgaaattcgt tgatacaatc atcgagattc 68460 aggattatcc attcccaacc atggaataca cggctaagaa acgtcgtaac gttggtattg 68520 gtctgatgaa tgcagcaggt gcaatggcag cagaaggttt ggcttatgaa ggtgttgaag 68580 cccgtaactg gattcaccgt gaagctgaga agctgtccta cttcctgcac aaagcctctg 68640 tacgtctggc taaagaacaa ggtgcatgtg actggtttaa tcgtactaag ccatccaaag 68700 ggactctggt aatcgacacg tacaagaaaa ctgttgatga actggtaact gttgatctag 68760 aaatggattg ggaatctctg cgtgcagata tcctgaaata tggtatgcgt aactccgttc 68820 tgactgctca gatgccgggt gaaagttcct ctgtactgct aggtgttact aactctattg 68880 aaccgcctcg taagattgta tctatcaagg gtagtgcagt gaataaagta attgcaattg 68940 ctccaggtgc aactgattgg gatacattaa tgagctataa actggcttat gatgtagatc 69000 gtatcgagtg gatcaaatgg gtagcaacca tgcagaaatt cttcagtcag tctataagta 69060 cgaatatgta ctatgactac accaagtttg agaatgaaat tattcctggc ccagtagtag 69120 ttcgtgactt catgactgct gttaagtatg gttggaaaac ttggtactac gctaacttca 69180 acactgaaaa tggtggtgga gctggtgaag aagcagcagg ttgtgcatcc ggtggttgta 69240 acctataata aaaacaagga gatcttcggg tctccttttt ctttgaaatt ttacttgcta 69300 atcgctacca gatttgctat aatatctcta tattaaggag atatactgtg aaacctcaac 69360 taacaaaaga atttctagag aaatatctag aactcagaga agatactctc tattggaagc 69420 caagagagac taataaaggt ggatttaata ctagatttgc tggaaaacct gtaggagccg 69480 tcaatggaaa tggctaccta tgcttccgac ttaacgtgga aggttataga tgggaattaa 69540 gaaatcatgt agtaatcttc tgtctctatt atggatatta cccaactaag acagtagacc 69600 ataaagatac aaataaactt aataaccatc ctttaaatct acgagaatca accgatgctg 69660 gtaatgccca aaatagggga gccggtaaaa acaataccca tggacataaa aacctagagc 69720 ctcttagttc aggaaactgg agggttagga taacacacaa cggtactgtt tacagatctt 69780 ccgcaatgcc tctagaaaaa gctctagaac atagagatat taaacttaaa gaatttcatg 69840 gcgaatacgc taataatgga gaataattaa tgacaacctt actaaatctg aactggaatc 69900 ctgattctga agagctattt ctcggagatg ccctgggagt agcagactat gttcgtgttg 69960 ctcatccaga gctagaacga ctcgcactat tacagaggag ccagttttgg actgaaactg 70020 aaatcagtct agaaagtgac aaaaagcaat ggcctaatct tccaagagaa atacaagaaa 70080 tcactctgct gaacttagct tggcaggttc aaacagattc tttcattagt cgtgctcctg 70140 aggcagctat tatgccactt gtaagccgtc ctgaattgga aggtatgctc aagcagtgga 70200 gctacttcga agatctacat agccgtgcgt atagtaacat tattcgtaac gttctgactg 70260 acccagcaga atttattgat tctgtaacta aaaaccaaga agcatttgca cgtatcgcag 70320 attctgttga actgtttgat gaattgtatc tgttaggtca gtatttcctt gcagtacgtg 70380 atcatcgtgg tgataatact tatccagaaa ctgagttccc tgaagttaaa cgtgaaactc 70440 aggctaagct gttagatgct tactttgcaa tctacggtct ggaagcaatg cagttctacg 70500 catcttttgc atgtaccttt gctctagcag agaacgatat tctgcaaggt attgctaaga 70560 acctgcaatt aatcgctaaa gatgaagctc tgcatactca aatgtctaaa gctatcatcc 70620 agattatgtt ccagcagttt gataaagatc tggtagatga agcattagct aaagcaccag 70680 cacaactcct gaaaactctg aaaactgaga ttgaatgggg tcactttatc tttaaaggcc 70740 gtagtctgat tggtttgaat gcagaactgc ttgaagaata cctttacttt gtaggacgta 70800 acgcatttat gcacattggt gtagaatggc caagccactt acctgtaatt actaagaacc 70860 ctattccgtg gattatgaac tggctggata ctacttcctt acagcctgcc ccacaagaaa 70920 tccagattgg tgcagcatat cgtgtgggtc aggtaactga aacttctgct gataccctga 70980 aagatctggg taacgaattt gagggcatgt tctaatgata gcagcaatct atgcaactgg 71040 gccgaatggg gagttcggcc tcaatggtaa gctcccctgg ggttctttca aagaagaact 71100 agatatcttc tatactactt tggattctct agcacctgat aatattattg taggtgcagg 71160 aacttggtta gccttccctg agactctgca aaaaagaata ctaggagatt ctgacctatt 71220 tatttatgca aatagaccac tacctaactc tgtaggatca gccacacgca taagtaacat 71280 tggatacagt ctacctgatt tcctcaagga agaagtaact gtagtattag ggggagcaac 71340 tctgcttctt gaaatgtatg tcaaaaatca tctagatatg gcgtatatct ctactattac 71400 ttcagctaca ccattatctg ctgatgtaca tctgcatcat cacattactt taactaattc 71460 aatggcaact agaatggatt atgtagcagg acagaatgca gataaaacgc tttcattctt 71520 acaagaattg gtgatattta aatgaagcaa tatattgaaa ctggaaaacg tattcttgaa 71580 gagggtgtat gggtaacgaa tcctagaaca ggggttcgtt gtctaaccat tattgatgca 71640 gactttacct ttaacgtagg agtaggagaa ctgccgctag tatctactcg taaagcattt 71700 gctaaacaag caataatgga aatgatcgga tatctccgtg ggtacgactc agcagcacaa 71760 ttccgcgcta ttggtgttaa tacatggaat gctaatgcaa atgaaaatat tgcctggcta 71820 ggtaatccat tccgtaaagg cgaggatgat atgggacgag tctatggtgt tcaaggacgt 71880 cattggacta attctgatgg tatggtattt gatcagttag ccaaagtcta tcataatttg 71940 aaaagaggga ttgacgatcg tggagaaatt ttaacttttt ggaaccctgg ggaatttcgt 72000 aaaggatgcc tacgtccgtg tatgcatacc catcaattct ctattttagg tgatacacta 72060 tatctaaact ctttccagag gtctaatgat ttcttgctgg gccaagtatt taatatggtg 72120 caatgctatg cacttctagc tattatggca cagattacag gtcttaagcc aggtattgct 72180 cgtcaaaaga ttatcaatct tcatatctat gaagatcagt ataatgttct gatggaacac 72240 aagcaattcg atcgtgaacc ttttgcatct cctactttag aaatcaatcc aaatattagg 72300 actttggatg atgtattaac gtgggtaagt aaggatgatt ttactgttcg tggctatgaa 72360 caccatcctg ctattgcata tccattcaca gtctaattca aatagccctt cggggctatt 72420 atatttaaat ttcatttgct tactgagtga aattcaaata taatatattt tataaattgg 72480 agacacttat gggactcttt aaaaagaaag tttatttaac acaaagtcaa gaacgcaggt 72540 tcttagctct gtctcagtat taccatttac ctttcgatca tttcatagaa cgggcgttag 72600 aacaattcct agaaaaggaa ttaagggcaa ttagacctga accgccaaga gcaccaaatg 72660 atgcacagag aggtcatcca cgtatcccac ctatgccgcc agttccacca gcgactaggg 72720 tcattataga aggtgaaaaa taatgtctat tcttttatct gctttagaag caaaacaaaa 72780 tactattgct agcattaaaa aggaagtaat tgacctagaa tcacttattg ccgtagagat 72840 tgacaaagct attaaagata aagaatttaa ctgtcacatc cagggattaa ctgaatactc 72900 agaaaacgct agaaatagcg ttatgcaaaa acttagcaaa ctaggttatg ttgtggaagc 72960 taagtatgaa cagcgtgaag gtttttggct tgaaattgat tggagcaaat aatgtcggtt 73020 ttccacatat atacagatgg tgcatgtaaa ggcaatcctg ggttaggtgc atggggattc 73080 gttgtctata atgataatga tgatcgtata ggatctaaat ccggctttag ccctaaaact 73140 actaacaacg aaatggaact cactgcaatt gtagaagttc ttcgttggtc tactaagaaa 73200 gataatcgtc cagtaattat ctatactgat tctgcttact gtaaaaatgg tatggaaagc 73260 tggatgtggt cttggcagaa gaaaggctgg aagaagggag atggtgaaac tcctcttaat 73320 ctagaactct ggcaagaagc atttaaacta actcaacagt atattaattt ccatgatacc 73380 aacccaacct ttgttaaggt taaaggtcac tcaggtattc gtggtaatga agaagttgat 73440 gctctatgca atactgtaat tactgagcaa gaacttgctg agatgtaata aataatttaa 73500 aaattcactt gcttaaagcc ctcgaattta gtataatatt cgtattgaaa gtgaggaggg 73560 ctttatgcga atttacaact caaattcatt aggatttaaa cctttgagaa aacgcgcaca 73620 atctccgcgt cagatcagaa aggcaaatat tggtgagcca gaaactaggc ttccaccacc 73680 tccggagcaa cgtctagttt acctagatga agaattagcc gaaagggaac gcaaagctca 73740 agaagaaatt gagcgcaaga aactatgtac tgctcctgca tataataagg gtggttatca 73800 atacgtttcc agtgaggaac aagctaagat gatcggcaga taatttttaa aaaattcatt 73860 tgctaaacgc ttcaaaattt cgtataatat atctcataaa ttgattaaca aaaaggaaaa 73920 caaataatgg ctaagcagaa aaacgcaaaa actcaggcag ctccggcagt taaaactttc 73980 gctcagaccg aagccaatcg taaggctcgt ctggaacgtc atctgcgtaa gcatcctgaa 74040 gatgttcagg ctgcacaagc tcttggtcgt ccggctccgg ttcgccagaa gccgaaggct 74100 aagaactcca ctcgcgcaac tgctaagcag gttgtgttca tcaagggtga aggtcataag 74160 tctgtaccgg taactctgag ctttaacggc ggtgctgaga tgttcgcccg caacggtatg 74220 gctctgaaag actacgaaaa agcagtcaat cagaagcgta agccaatggc tgacgtaatg 74280 cgtgaatccc gtggacagtt tggctccgtt aaaccgaata ttttcggtgt agagtatagc 74340 cgtgacaacg ttcgcgctct gtgctacggt atcggtatta agtttactgg tgcttctgat 74400 cgtaaatccg ctaaacctac tcgtaagcgt aaagcgaagt aatgattgat ggggcgaaag 74460 ccccatatac taactctttc tctgactaat taaggatatt tttatgttct ctaagaaacc 74520 acgttccgtt actgaaatcg ttgcttcctt taccactatt actgatgaac ttcaagctcg 74580 tattgaagct gaccagaaaa ctgctgctga tattcagaaa cagcaggaag aactcgctct 74640 gaaactggct gaaaccaata agagtgaaaa atctgctcaa actattaagg aaaatatcct 74700 taaactgctg ggcaagtaat ttaaatcagg gggcgaaagc ccctcttatt tggagaaact 74760 atgcgtaact tcgttgcaaa gaatgatttt aaccgtgcta gcactcacaa gtctgctaga 74820 gactacactc gtctttctaa acatgagctt ctagaagaaa tcatggaaga aaactgggat 74880 gttagtgaaa atggaacaga cctgtttgaa gaagaggaac ttcctctgaa agaccccaaa 74940 cagtgggagt ctattaaaga gacagctctt taatagtttt ctacaacaat aaaggacata 75000 attatgaaat tcaagccctt tggtatgctg gacattgaat ctctaggtac tccaggaaat 75060 tgcggtacta ctcatattgc aatgccttct ttcgcttttg tagcgatgca cggaattgat 75120 aaagatcctg atctggtatt cgttacgcta gacgttcaag atcagcttaa tgcgggtgct 75180 aaggtaactg cctctactat tgcattctgg atggatcaag ctaaaaacag tccatctgcg 75240 ctccatatta tggaggctct aaatggtcgt aatcctaaac tgattgcctt ccgtgatggt 75300 aagcatcact gcactaatga cgtaggctct aattatgctg ccttctctat ggctcagaac 75360 attatggaat tgtctctagg agataatgct ctgtactacg gcaatggccc agagtttgat 75420 atgacaatct actctgcaaa tacgttccat gccggaaccc atgaagaaat agttccgtgg 75480 aaattctgga acttaggtaa cgttcgatct ttccgtaatc agtggatgca agctggttat 75540 agctataaag atctagaagc cgaaggtgct agctgggcaa aagctaaaat ggaaaagatg 75600 gacaccattc gttatggaat ctatcctgtg aagcatgatc cagcattcga tgctttagtt 75660 gaaagttatt gtgtagccgt catgatcgaa aaaatcaaaa tttgatttgc tttcagctcc 75720 aaaattctgt ataatatatt cataaatttg agagaacaac aaaaggaaaa caattatgcc 75780 agtatctaaa aacgtccgta aaaatggtaa agtagcaaaa cgtaacctgg gtctgcgtcg 75840 tatggctgaa cgtcagtccg gcatccagaa aatctttaat gtactgaatc gcgccaatcc 75900 gaatattgat aacagttctg atactgttct gcacactatg ctggcaatcg gtctgtaata 75960 ggccgttttt aaacaaattc aaatctcaaa cttcatataa ggaagcaaat aatgactact 76020 cgtatctcta aaactcgtgc tctggcaact atcaagtctc tggaagctaa aatccgtaaa 76080 gctactgaac aacagctttt gatcgatgtt ggtgaaggta aggacaagaa ccaagttgtc 76140 gttggcgctg caatcgaagt agatgctctg tctacccgta ttaagactga tttccagtct 76200 ctgctggata tgatgtccca gcgtgatcgt ctgaaagctg cgctgatcaa gagcaatgct 76260 gaaactgttg tagagatcgg ttcgcgctct atgactgttg cggaagcaat cgaagctaag 76320 cgttctatgg aactcaaagc tcagttgctg gctaacatgc gtaagcagtt ccatgctgct 76380 actgttaagt ttaacactca gaaagctcag tttgatgcta agtatgagcg tttgcaggat 76440 accatggcta cccgcgacaa gaagacttcc gaggatgaag ttaagatgca gcttaacctg 76500 ctggaactca agaacactcc gttcctgatc gatccgctgg ggctggaaaa gctgatcaag 76560 caacacgacg aagagtacca ggactttgca actaacgttg actttgttct atctgagtcc 76620 aacgcctcta cttttatcga agtagagtaa taatttaaaa gactagtaaa tcggtagacg 76680 aaagcccaag catcttacct cgcagcagcg gttgaaatac gaggttaaat aagaccgctg 76740 accatactat aaagtataat ctattggaga ttatgtttat tacaccagcc tcttaagctg 76800 aactgtaaaa acggtcgttg gtagtaccaa cacaacttca aagatcaacg ctcaagtatg 76860 aaagctgaaa agttaaaagc actcaaactt ctaaccttct aaagctaaaa gatgcaaagt 76920 attcgttgat gattcaaacc ctagatcaaa ggtacatggc tcataagata tggcctgtgg 76980 cgcctctagg ctgtttatcg gtttactagt ctctcaattt aaaaatgtat ttgctaatta 77040 gtttagtttt ctgtataata tatttttaaa ttgatgagac gaggctatta tgatttttca 77100 tattctgatt gatgatgttc gtgacctaca tgggatggat attatcattc gaacccccga 77160 agccgctatt gagtttctga atacaaccga taccaccgga catttcgttt atatggataa 77220 cgatctaact gtagatggta tggaaggtta tcaaattctt cgtttactcc tagaatttgg 77280 acagagaccc aagaaagtgg tactagtaac ctcgaaccct gtagctaagc agaacatgcg 77340 caatgatttg cttgacgaag ggtatcaaga aagccctaat cgtattgagt acgattggcc 77400 ggagtaaata aaatgaaggc agctttgctt ttaatagctg ccctaaccta tagcttcaat 77460 agtcatgcga cctacgactc aaagcagatt gattgcattg caaaaaatat ctacttcgaa 77520 gcacgaggtg agggcactaa aggaatgatc gcagttgcac aggttactaa aaaccgtgtt 77580 aactctggta cttttcctga ttcttactgt aaagtagtat atcagtctaa ccagttctct 77640 tgggtggcca agagaccaaa agttgataaa accgatgagg cttggcaagt tgctaagaat 77700 ttagctagag tgatatacta cgttgattta ccacaagatc caacacatgg agcgttatac 77760 ttccatagtg gtaaagataa accgtactgg acaaagaagt ttaagaaaac aacgaagatt 77820 caaggccata cgttttacaa gcctgtggtt aagaaaactt aaaaaattcg attgcttaaa 77880 gccttaaatt tctgtataat actttcataa attaatgaga ggaagcgaaa tatgaaatca 77940 gttgtaatta ttggtgttaa tactcgtggt atccgtacta cgaaaacagt taaaactact 78000 aacgttgctg aaattactac aaaccctaag aagtttgatt tcgccaaagt ctcggcagtt 78060 atgacagaac aatcttttaa ccaagcactg ggaattaaat aatggaagag aatattctag 78120 atgttttgcg tagggctttg caggaatcta aggttgatgg ttctgataaa gaagtagcta 78180 aaagctacca gaccataatc ggagatcttc agcgtgttga taaagacttc attacttctg 78240 aacagtttgt atcttatcta aaagctcaac tgaaaagcat taatcagacg aaagctaaac 78300 ttcacggtca agaccttgat agttatagct tgcagtctgc tcagtatgaa tatatactga 78360 acaaatggtt acaacagtat ctgccgcctc agctttctga ttctgagatt cgcaaatact 78420 ttgcagaatt agttaagttg aaccctggga ttacaaaagg tatgctgatg aaagcgatta 78480 aggaagagtt tcctggtcgt tacgacggtg gcattgctgc tcaggttgct ggcgaattta 78540 attaaaaatt gatttgctaa acgcctcaaa attctgtata atagattcat aaattagaga 78600 acagcgagcc gattacgctg agtgagttta gtgattccta ctaggatagc tattccactg 78660 aatagagtcg gtgagtttag aatgatcacc tgttagagtt ccgagtccag tccggtatat 78720 ccggccaagt gactagagta acgaatatag gttcgagact tcttgtaaaa ctcgggagct 78780 gtcccgaaac ctcgtaaact cttctctaat ttatacaaaa cgatggttct caacatgagc 78840 taggcacagt gtgaaatacg gtgcgtgtcc caggggtaga agactgaagg ttgggcttga 78900 gaaacccatg taactttcaa cgctgacccg tctaggatgc aagtcaagac gtatgtatac 78960 cacaatgcgg cgattgtcca tcgaatagtg gggtgtcgaa ccgacttatg tgataacact 79020 gccagatgct cacaggctga cattgagctt cgctgctcct tctggaaaga atcggataac 79080 tcgggcttat accggataag cattcgctgc tggagtacat actatgacgg ttcaggaggt 79140 acgccgcctg atcagtatta ggttagaatc gaaacagcta aaccacgagg aaacctgacg 79200 gcctcaacca aactcggaac tcgggatcac tgcctagtgt gtgccccgtt taacatagag 79260 actaggcatc cagcttcgca tcgaaaatcg gaggtgcgat tctaacagac cgcgtgaata 79320 tgagagtggg aaatgctggc atgagagatg tcactaatgg tgcccaatag tataggtaat 79380 tcgcgataat tcctgttgag tggcagcagt aggctgggag tcatgaccta agcctaaccg 79440 agttcttcac atacggagaa cataaccaca aagtcaccct gacttaaact ggaggatatt 79500 atctgacgga agggctgcag agcttaatct gagggggctg accctaccta ccgtcgggta 79560 tctttaagag cttttataag agttcttaaa aatacctaaa cggggtgtag tctaagggat 79620 aggcaggagt cttctaaatt cctttatgca ggttcgaatc ctgtcacctc ggccaatttt 79680 attaactgct aggaaaataa agtgaaaaca cgatatatag taaaagtagt aggtgaacat 79740 tcacaagtaa ataccaccga tgttacagcg ctagcatttg aagtagtctc cggtatagtc 79800 atatttcata acggagatat caaagctcct gaatttgctg tggatgctag cctgtttatt 79860 tctgcaacta aagtaactgt agtagctaac tcttctaaat aataaggaaa tatatgttta 79920 ctaaaaacac taaagaactg acttccgatc aaaaagtaat tcgtactatt aaacgttggg 79980 gtgttggtgc tgtagttggt cttggcgctc ttattctggc tctcaattcc tatacagttg 80040 ttcaggacgg tacggttaaa actcagactt tcctgggtaa agttagtcct aatcctgtat 80100 tgccggggtt ccatattgtc aacccattcg cctcttttga tacttttagt acgaaagata 80160 tttctatgaa actggataaa cttcaggttc ctagccagga taagtttaag tctacagttg 80220 acattaccgt tatgttgcag tttgatggtg ctaaagctcc tatgaaccgt gttaacgcag 80280 gtactcagga tcaagctctg aacaagtacg ttgaagagaa gatgctgtct acgattcgtg 80340 agtttggtaa gtctgtacca aaagcacaag atctgttcga cgctaagatt caggctcaac 80400 tgcaaaccgc tattcagcaa gaagtagaag agtacgcgcg tccttatggt tacaccgtta 80460 agcaagtatt ccttcaggat attactctgc caccggtaat catggaacag gtacaaaaca 80520 caaaagttcg tgaagaacaa gttaacgctg caaaagctga actggctcgt gttgaacaag 80580 aagcacagca gaaagttaag caagctgagg cagatcgttc cgctcgtgaa aacatggcag 80640 tagctaacga acgtgacgca gatgctaaac tgtacgcagc taagaaagaa gctgaagcta 80700 acgccgctct gcaacgtacc attactcctg aaatgattcg ttggaagcag ctggaagttg 80760 aaatgcttcg tgctgaaaaa taccaaggtg gtgtaccaca gactgttgtc ggtactggct 80820 acgatggtca aatgatcatg gatatgcgta ataagtaata gttacacgta atcttcgaaa 80880 caagcccgcg tttcccccga gagcgcgggc ttttctgtat ttaaggcaaa agaaaagccc 80940 ataagttatt caactaaggt ctttagtatt taattcttta gaatcatata ctattattgc 81000 agcaacaacg tgctaccctt aagtgccctg cgattttcca ctaaaaagtt taaaaattca 81060 attgcttgaa tgctaaattc tctgtataat atatttataa atttgagaga ggaatcaaat 81120 atgattaaga ctggtctagt aattggtaag ttcgcaccat taacgcgtgg tcatattaac 81180 ttaattaata cagcagcaac tatgtgtgaa cgagttattg ttgtagttag tcatgataat 81240 cgttggctga ataagcagaa cacacgtgat caggaagtat tacagttaaa gaatcgactt 81300 cgttggttag aacaaactta cgcggatatt gagcacattt cggtagagta cattattgaa 81360 gatgatattc ctgaatatcc gaatggttgg ccggaatatg ctagaatcct taatgaaaat 81420 tacggacatt tgcctgagat caccattttc tctagcgaac tagactatga tgcagaatat 81480 aagaaatatc tgccgaagtt tggtcattat attgtagatt ctgatcggac tcgtgttcca 81540 atctcagcta caatgattcg taatgatctt atgaagtatt gggagtttct tcctagcgta 81600 gttcgtaaag attacgtgcg taaggttgta atcattggga ctgaaagctc aggaaagact 81660 actttaacaa aataccttgc gaagctatac aacacttcgt gggtagaaga atatggccga 81720 acgtattgtg aagttgatct agttggtaac gaaaatctgc ttaccagcga agactatagt 81780 aaaattgcgt tcagacacaa agagctagag tttgaagcag aacgtactgc aaataagatc 81840 atgtttgtgg atacaaatgc ttttattact gagttctacc acagacttta tgaacatagt 81900 aagaaccctg tggttacagc aattgcaaaa gaagaacatt acgatttagt aattgttcta 81960 gcgcctactg tcaagtgggt agatgatggt ttacgtatta actccgatcg ttcaattact 82020 tctgaactgt tcgagcagat gaagaaagaa tttccaaatc agttccctga agggcgtact 82080 gtttacatcg actctccgga ctacaaagtt cgtatggacg aagcagtaaa cgcagtagac 82140 tggctgattc atcactttaa cgttggagag ttttaatatg actaactatc tcgtaaatga 82200 ctttaaaaac tggaaacctc tggaatatct gtggctgttc atagcggttg tcttcattag 82260 tttagcaaac tataatggta cagctctaga ttgggctact gcaataacca atgtggtttg 82320 tgttattctg gttgcaaaag gacgtgtttc caactatgtc tggggtttgg taggtgttct 82380 gacctacggt attacagctc taaatgctga tctttatggt aacgcagcac taaacctatt 82440 gtactacgtt cctatgcagt tctacggaat gtacttgtgg cataaaaata agccaagtaa 82500 tctaccggat gttccaacta aagttctgac aaaagctcaa gctttgctga ctctagggat 82560 tttagcagta gccacgttag gttatggtag tttccttgct actacggctg atccattccc 82620 aatgttggat gcatttactg cggttggtag tgtgattgct atgtggctta tggtcaaaca 82680 atatgctgaa caatggctga tttggattgc tattaacatt ggtactgtgt acatttggct 82740 tgtaactgct caatctacag gtgcatctta cgcaatccta gcaatgtggc tagtattctt 82800 aggcaactct attcttggag cttataaagg atactttgta cgcgttaaca aataaagtag 82860 ttatagccct ctaagcgagg gctattatac agaaattttc acttgcttaa tatagaaaat 82920 ttctgtataa tatatttata aattcgagag aggaatcaaa catgaaacgc attgtacttg 82980 cattgactct agtatctact tctgtatttg ctgctccgca ataggaactt tgtcaagttc 83040 aggtttgtaa taagttagaa cgatttactt tggatatttg gtcacacatt aaagataata 83100 tgggtgaaca gtgttttgat attgttctgc caaaagagca ggctgttcct ggcgctgttc 83160 ttagctcaga gtcacgttgg tggcaaggtt cttctatcaa tccaactaag aagtcagtga 83220 cacgtatcaa acaagtgtat cagtgcagtg ttcagtaaaa tttcacttgc ttaatgccga 83280 aattttctgt ataatatatt cataaattcg gaagattaac cctaaaggta agggagctgt 83340 ttgctaaaca gccagtagct gtggagacac ggtgtgccag ttcgagtctg gcatcttcct 83400 ccaaatttgg ttctcaagct catttggtat gagccgtcgc ctcataagcg aaaggtaggt 83460 aggttcgaat cctccgggag ccaccaatta actaggagac tatatgtcta gattctatga 83520 aatgaagaaa gcattagaag aatgtcggtg taaggattgt catggtggtg gtaggcagga 83580 tgatgcagaa cctggggata ttttctgtag agaatgggaa tgtcctacat gtaaaggtac 83640 tggtatcaac cctgaatatg caatttgtct agacattgag cgaggctaag atggatattt 83700 ttactactcc ggcaataaac cttttaggtg ttggcttgtt ccaagctacg gtgtatcgta 83760 ctgatgattc tactgatgtg gtaacgttca tcgtacctga gttcttccta gaaaagttct 83820 ttgaagagtt tgagcaattc cgtgaagaac acaatgctca ttccgatatg gaagatctag 83880 cagctatgtt ccctgcggtt tacggctata tctttgaagg taatggccta gatcttaata 83940 aatctgaact cgttgaactt aactggggca tcaattttga agttggctct ccattccctc 84000 gatatttcca gggtttagag attcgctaag attacatggg ggtgtaatcg aattggcata 84060 ggtactggac ttaaaattca ggttttgtgg gttcgaatcc caccacccct accaaattaa 84120 ggaaataaaa tgaaagctat taagaaagca ctacgtaaat ttatgtactg tcgttttcat 84180 agcatcaagg cttcaactaa tggtacgact tacgatcgct atgatcctaa gtttggctta 84240 cattctaaat aactggagat aataaatggc taatcctgaa attacggcac aacgctttat 84300 gctgaaagga attattgctg aagcaggcat gacagaagaa gttgcagatt ttcaaaagca 84360 gttcctaaat attctcactc aagccaaaga gctaggagag aaagaacatg gcgcggctat 84420 tatggcaatc acattggttg ggcttgatct ggcagaagaa tcaggagttt aactcctcca 84480 agcagaaatt ttaaaaatgt acttgctttt tacttaagat ttctgctata atagttatat 84540 aaattgatga aggagttaac gaatgtcaaa acgtgttact gcaactggtg ttgaaagtgc 84600 tgtttgtaaa tcctggggtg gctgggaagg tgagattgaa tggctttatt tctacgatgt 84660 agaacttctg ccggaagtca aagctaaatg tgtggaagct ggtatggctc ctgatgcaaa 84720 ggctgatatc gaaatcacca tgactaagct agaaggtcgt gtaattacgc tcaatgatga 84780 ctatgaagaa gtgttcgagc ttccattcaa actgagtgtc actcctagct tcgagtaact 84840 gatatagcac tgagcaagtt ctttaaaatt tgacttgctc agtcctttaa aacttgatat 84900 aatagttatc taatttggaa aacatatctg aatgagaagt aactaagcga gttgctttta 84960 ggggtaaagt caggatttcc tgaaatattc caccttgagt ttaaccgact tacttttcat 85020 tcaaatatgt tggattagta tcgtagaggt agcgaagcag actgtaaatc tgccgactcg 85080 gaagggtctc gggtggttcg actccatcat ccaacaccaa atttaagtcc tgttagacaa 85140 actggtaaag tcactaccct ttcaaggtag gatttgcggg ttcgatcccc gcacaggacg 85200 ccaatttctg cattcctaga gtgttactgg acagcatgtc ggtctccaaa accgtacggt 85260 ctaggttcga gtcctagtag gtttgccaat atttgcacct ttagctgaga tggattagcg 85320 cttgcctgaa gagcttgaga ggttggatca ttaccaacgg ggtgcaccaa tttgaaggaa 85380 atgctgatgc atcctgaaaa cttctttatt tactgtgcgg ataagtcact gaaacgcaga 85440 attaacaaag tctatcatta tcgtgctagt caacagcctt atgtttatgc ttttgacgga 85500 gatcacatcc cactagaaca gttggcctgt gattacatgg tattgaagaa ctttgacgag 85560 acaaagatct caagcgttca taatcgacag aacccgttaa cgtggaaata taagaagaaa 85620 aagaaaggca tcatatttct gaaaaatgta gttgcttaac cgcttaaagt tctgtataat 85680 tattacataa attgaactgg agaagcaaca tgacagaaca agaactgaaa gaacgtaagc 85740 tggtaatgct tcgcttactt aacattttct ctggtatgga tggtggtatt gacttcattc 85800 aactacgcac ttttgtagaa gaagcagaag gcgaggctat cactaggatt cttaaacata 85860 tgggtttgtt actagatgct gctaatgtgg ctactcaata taaactcgaa gatttaccgg 85920 agcgtgttaa atgaaaatgt tagttaaatt taagtgttgt ggttctgaac atatctttac 85980 acgtctatgt gagtttaaga tgtgtaagtg tggtaaatca ggctatgatg ctggagatgg 86040 ctattatgct agatctatag gacattctaa agatctagag tttactaatc tcgaaaagca 86100 agataaatcg accgttggct gaatggctta ggcggaggcc tgcaaaacct ccttatgtga 86160 gttcgaatct catgcggtcg tccaaatttt agggtcgtta gccaagcggt ttggcggtgg 86220 actgttaatc catgtcgaaa gacaacgtag gttcgaatcc tacacggccc gccatattct 86280 gtgtagagtt aagtaaaccg gtagccaacc agctatcatt actgatatcg aattcagtca 86340 cagataccat tttcattaat aactgaggaa atagatatga caactcgtgc aattgctcga 86400 ctagaagctg atcgtgataa tctgattaat aatattaagg ttcgtggtaa tattattaag 86460 gctctgaaag ctgaacttct tgaagaactt caaggtcaag tagagcgtcg tgaagctcta 86520 aaagaggttg aggaagctat caaaaaactc aaagctgagt aaacaaatta tgcgatcggg 86580 gctggcttgg taatggtact cccctgtcac gggagagaat gtgggttcga atcccatcgg 86640 tcgcgccaat ttaggatata gtatgacacg caaatattta gttattaaag gtgaacatgc 86700 aggacaaact gtatactcat tgaaatttca tgattatggt ttagcctcag atgatactcg 86760 atatactgga atagagcata caagtgtaac attagacgaa acaggtgatt atcctagttt 86820 tactattcct aaagatttct taaaagagat ttaatgcttc catagctcaa ctggtcagag 86880 ctaccgcctc ttaagcggag ggttgaaggt tcaagtcctt ctgggagtac caaacaaata 86940 gagtaaaggc tacagtaggt gcacccttac ggactgctac accggactct attcattaac 87000 aaacattcta gcgtggtgga gaggggacac cagcttgccg agggtagaag ctcgttcgat 87060 tcgagtgaaa gaatggttct cgtaaatccg cacaagtgat agcaggttca agtcctgcct 87120 agattgactt attatgcgtt cgtagttaaa aggtataatt tttggttgcc aaccagaagt 87180 tgagggttcg attccctccg accgcaccaa atttaggttt ctgcttccta gagaacggac 87240 tcctagataa acaaggcgat gagtttccgg ccggttctag tcgctgaaaa tcctcccgac 87300 caggtaggtg taggtaaacg aaagtttact tattgagtag gttccgatag atcggcgggt 87360 ctattctggc atttaagaga agtctcgcaa acttctttgt cagcagagcg tttgaagcgt 87420 gaggtggcct acgcaataag taaatttatt aaaaactcac ttgctattat gcttaaatat 87480 ctgtataata tattttataa agtgagttaa caaagaatat tctccgtgta gctcagtttg 87540 gccagagcgt tccgtttggg gcggtagggt cgggggttca aatcctccca cggagaccaa 87600 acaattgcgg gttagatctc tggtagagat cgctagtctc ataaactaga aagaggtagg 87660 ttcgattcct acacccgctt ccaatttaat agaaggattt cgaaatgaaa ttatttcgtt 87720 taaaagtaag tgatctacgt tctagcaaag atcgtaagaa gtatccacta gcccattatg 87780 gaaaagatga aaacgaagtg atggcggaat ttggtattaa tcatccttac ttgaaagtct 87840 attctattaa agaagtatac atgtattaaa ttgaccgtta actcaactgg ataagagtac 87900 cgagctttta cctcgtaggt tctgggttcg aatcccagac ggtcaaccaa tttaaaggaa 87960 tttgttatga atattaacaa agttctagaa acaatatcag aaatgtctgg cagagccgga 88020 aaacgcttta ctgaactgtg tattaaaaat caaaaagtac cagaacatca gtgtcattat 88080 tgtcgagact gtgaacaata ccatgaaatg tgtatggttg aagattggat ctgggatcag 88140 ataagaaaag agcagcccaa agatattgtt ctatgtttca cttgtatgga acgacgctta 88200 ggacgtaaaa ttactcttaa agatcttacg catgttccgt gtaatgcacc ttacttcatt 88260 ggctttagaa tggagagatt ttaatgacta tctggtttta cttaatgaag cagaaaataa 88320 aagattccta tgtattctct gccgatttta caataggtat gtttttagca ggtttctgtg 88380 aatttgtagt tgaagcctat attataggac gtcttttgct cggttagttt aatgggagaa 88440 ccccgtcttt acacggcggt tgcgatagtt cgattctatc accgagtacc aattaactca 88500 aaataaggaa ttgcaaatgc aattatcaag caaggttcaa ggctatttcg gtcatgtaac 88560 aatggagttc gctatctcta aaggttggga tttcgagcaa gatctagctg agttctacaa 88620 agatattgat ctgaacgtac catacatggg actctccact ggtcaagaga tcacagaagg 88680 cgatgtgttc aaggcaagtc aaggtgaggt aacacctagt tttgccaagt tctggttagg 88740 acttactttg aaggaagatg caccagagga tggtgtggtt atgcagagta tgcactctgg 88800 aagctctgat taccttttcg ttagtgacgt taaggaacta caagaaatcg ctaaaaatgt 88860 acttgcttga tgcttaattt cttgttataa tatttatata aattgatgag gagaaatcct 88920 catcaaagtt cactaaggta ttagctcgac ctaggactgg ctgccaaaaa gcacaattgc 88980 aattagaccc ctaatggcgg gggatacttt gcaataagtt ctagattagt ccactagatt 89040 cagcggcaac tgaatagtgt atcgggtttg tgaagagcgg ttatcctcta ctggctaatg 89100 tctgagggta atacgcacac cgagctaata gccttagtaa acttaaagat tttggggata 89160 tagtttaatg gtagaacatc tgctttgcac gcagaagacg agggttcaac tccctctttc 89220 tccaccaaaa ttcggggtca taggttattt ggttaaactt actgccttgc aagcagtgga 89280 actcagttca attctgagtg actccaccaa acaaatgggg atgtggcgaa attggcagac 89340 gcgctagatt taggttctag tcttcgggtg tgggttcgag tccctccatc cctaccaaac 89400 attggattaa ctcatgatga aaactcctat aactgatatc tggttagctg ttaatacatt 89460 aactaaagag tatgtgttta tagagtatgg tcagaggtat gaccaaaaga tgtataatct 89520 catacttcca gtatctgctg aggaagttcg tgatttaatc cttaaaaatt cggaagagca 89580 aatcgattag gtgacgaaaa ccgcttggaa agtggctgac tggtaaaacg gcttgagagt 89640 tcgaatctct cttcttccgc caaacaactg gaaggtagga catagtggta tgtaacaggt 89700 cttgaaaacc tgcccgctgt agcgatatgg tgatggttcg actccattac cttcctccaa 89760 ctaataagga aaatcttatg aaacacattg ttaagtatac cgaaagcgaa cgtggctggg 89820 gtggtgaaat ctggtatcgt ccatttgaca ccgaggccga agccttgatg gaagttaatg 89880 atgtaaataa agatctacct aaagatcatg tgccaacata ctacatccaa gctgagtata 89940 ttggtgttaa ggataaggtt cccgaaggtt acaagtttta aataattctt gaagtatgcc 90000 ggaatcggta gacggggctt cctaaagggc gtaaggggaa catccactcc tcataagata 90060 cccgaaaacg aggctgagat aaatggtcta tctcgtgaag gttcgagtcc ttctgcttct 90120 tgatattcaa tggctcaaag agtaacggtg cacctttcta gagaatttcc tgggcgagaa 90180 tccaggtgta atcaactagt tgttagtgtg atgagtttcg gagagccgcc acaattgaaa 90240 attcgtactc gtcttgagcc tcgtgcaata ggagataact tgattagtat tctagaaaat 90300 gctatttgag gatggtttcg ctaaccctgc gagttttcaa ttgtggctat atcataattg 90360 gttaatgatc ctgattgtga atcaggccta tgtggattcg aattccacta gccaccccaa 90420 atattgcgtc cttatttcaa cggaaagaat gtaaagctac gaactttacg atcggggttc 90480 gattccctga ggatgcacca attaacttaa acaggaaata aaatatggct gatttctgca 90540 aagaatgctc tatcgatatg tggggcaaag atacaggtga tctttctggt ctgattacag 90600 aagccgaagt taaagaaggc tatggtgctg ttgtaatctg tgaaggttgt ggtattattc 90660 gtgttgatca taatggtaaa cgtctagaag aaccagatcc aattgccacg gttcctgtag 90720 aataaaaatt attgggatgt agatcaattg gcagatcgtc ggcctctgac tccgaaggtt 90780 ccacgttcga tccgtggcat cccagccaaa tacttggaga gtagtgtaac ggttagcaca 90840 acggcctttg actccgttaa tggtaggttc gattcctcct tctccagcca atttatgcgt 90900 gattagttca gtggctagaa taactggctt ccaaccagta gacacgagtt cgactctcgt 90960 atcccgcacc aatttaagga aacgttatgc acgtttttga gtttcgtaat aaagattcac 91020 gtaacggtgg agttgaaggt ttacgtacct taattatagg gaataactac cgagcttatg 91080 aaaaagatga tggttcagta ctcctaaatg atgaagaacg tggctatagc ttcttccctc 91140 aagaaactta tgaagagttt cgtaaacaat ttagtctaga ctggagaaag ctagttacta 91200 gcagttcgtt acctgcttct atcagtatga gttcactata aatggctgat aatctaaagc 91260 taaaagtaga taaacaaatg aagtgcgttg aggttgagtt gggtgaagaa cgtttaaaac 91320 tctccttcga attcttacaa cagttggcta tgacagtaat gtctgataag tctcctgtat 91380 ttgaaaaaga aattccgttg aaataacggg tttattgctc ctaaagcatt gctggcgatg 91440 cagttgcctt gtaagcatct gaaccgggtt cgattcctgg tgggagcacc aaatttaaaa 91500 attcatttgc cttctctctt atttcttgtt ataatattta tataaattaa tgagagggaa 91560 accaaatgaa acgttatctt tctgtcgtat tccaaacggg tggtcaacgc tacacttacg 91620 aatttcctga gtcttggaaa atcaaagagg gtgatcaagt tgttgtgcta acacctcgtg 91680 aaggcttcaa agtagtaacg gttaaacaag tatttccaaa agatcacgaa ccatctaagt 91740 ctattaacta taagatgatt catggtctgg tacgaaaagt acctagaaca gaagtcgaag 91800 tagataaaac tggagaagct aaataccagt atacctctta cttagacgaa atgatgtaat 91860 gctctgatag ttcaacaggt tagaacaggc gaccgataat cgtcaaatct tggttcgatc 91920 ccaagtcgga gtaccaaatt atagttagtt ggcagagcgg ttatgcacct ccttcatacg 91980 gagtgactac agtggttcaa atccactact aactactaaa cagaggatca ggtcattgta 92040 ccagatcctg ctgagaagag agtccacatt ctataaatgg ccaggcgtga cagcaggaag 92100 tagactgcaa ctccatgcta tccaggagta taaatgtaga agctaagact ttagatagcc 92160 attgtcttag cgtgagcaat cacgtagttg caacagacca ggaggatcgc aacgttaaaa 92220 tcaaagctcc tgtgcttatt tatctaatcg agaccatcaa tctcaccgga atgctagcac 92280 cccaacagta gctagtgatt agataataag caagcctgcc ttccagtttt tatcttgctt 92340 ctgggttaac aaaaagctga gccaaattct aacatcaggg agatctaatg aaagcatatc 92400 aaagtcttaa gtctggcgta attaacattg ttcataacaa tcagatcatt cagctatatt 92460 ctaaagatgg tgaacttaaa cagaaagttc tagtcgaaga tctagaaggt atcacacctc 92520 actttgaccc tgaggccttt cgcattgtag atgtggaagt ggttcctcaa gtcgagggtg 92580 gtcagcacct aaatgtcaat gtgttaagcc gtgatcagct tttggatgca cagaaacatc 92640 ctgaaaaata tcctcagttg accatccgtg tctctggcta tgctgttcga tttaacgcat 92700 tgacgcgtga acaacagaac gacgttatta gtcgtacatt tactcaggtg atataatggg 92760 gtttggatac gatttctccc agctacgcaa ggtagtcgag caaactccat ttatctccca 92820 agttttagga gaaatgtgtg gatcgagcat taagacgcca tcaccggcaa cgtgtgaaaa 92880 acaatcgcaa gaagtattgg acagtcttcc cacatgaaga aagccctaag cggttaggca 92940 ttatcactac cactccttgc atctgttctt gttggatgtg tgggaatcct cgcaaatatt 93000 ataagaatag taaagcaggc atgaaaatct cggagattaa aaagatggat gcaatgatta 93060 tgggcatcac ctttacaggt ggtatctctg atgaattcga cggttttgta actcaggata 93120 ttgtcgaaat tatctaatta ataataaacc ccagctattg aataagtagt ctggggtttt 93180 cttgtatgta ggattttcga aatgaaaatt tttaaagtga actatagagg cctgtggtta 93240 ggtggtacag ctattgttgt tgcggaaact gcggaagaag caatcgaact ggctagaaac 93300 catagaagtt ctatagaatt cgaggatgtg acagtagaag aactcccgag cagtggggta 93360 gtatacaacg acaacggaga ttactgatgt tatattttga agttcgccca cattaagtta 93420 atttaaaaga cttaatggag gtaatatgag ccgtacatat cgtaaacaat ctggtgatcg 93480 tcactggcgt gatgcacatc gttctactta taattttcat agcactaata tccgtattcc 93540 gtatatctac acggataggg atggtaagca gtggaagtat tatcaagttc gatccaatga 93600 agcatgggct aaaacttggg atgaaattga gaaggagatt caggaaaata tcatccgtaa 93660 cgagaagaaa gatgggcact ggtgggattc tgttagtagg aatgttaagt ggcattctaa 93720 caaaatggtt cggcagggaa accgtcgcga acttcatcgt gtgatgaaag atccagaaaa 93780 ttacgactat aatcgcgatc atgatctgcg taagcgtggg ttatggtggt gctacgacta 93840 atttcgtaaa taattcattt gctaaaaccc tctccttttg atataatatt tatataaatt 93900 gatgagaggg ctttgaaatg aaaactcgac tagactgtgc tgttcacgta cttgagcatg 93960 atgttgttgg gacttgtatg tcttatcatg aagcgggtaa aatgattgtt aaacgtgttg 94020 ctgatgtttg tagtttgaaa attacagaag atgaagcaga cgaactactt ggacaggcaa 94080 tagagaaaac agaaattttc ttgtgcgatt cttgttcttg gtggtgtgaa gctcacgaac 94140 attctttcaa tgattatgat gtatgccgtg actgtaaaga ggacgatgaa gatgaataag 94200 tttattattg ctctagtaat ctcagcagtt agtttcggtt ctctggcatc taccaaagtt 94260 tctatgaaaa atggtaatgt taaagttcag cagaacggca ttaccaccga atatggtaaa 94320 gtacgtgatg ttaaagaacg taatggcaaa gtagaaatct atactaataa aaacttctct 94380 actccggcta ttactgttag taaacgtggt gaaatcacta ctcaacgtac taatagttct 94440 gattctttta cttgccgtta tgactgcggc tttgaaggtg aagatgagta atttagaccc 94500 tagaattaaa catgcagtcg aagaaatcca agatcagata gatgaggact tcactgtttg 94560 gtcaagattt ggcaatggtg aaggctgtca gctttatagc atgaaaatgg gcatctcgat 94620 tgaacttagt atcaatccag agggcaacgt agaagcccaa cccatgttca gtgttccagg 94680 cttctctgga tttgttatgg gcatgaaatt atgtttacca aataaacatc tttaccgtgt 94740 tgtctgccag ctagaaacga ttaagcattt cttgcctgaa ggtaacatta acgactacta 94800 ccatgaagtt gtagcagctt acatgatgaa agaacgcaaa cgtcggagag aagaacgtga 94860 aagagctaaa tctcaacagt ctagtacaga ttccagcaac tgatgctgta ctagacttcc 94920 tcaagcagga acatattaag ttctggacta actatgcaga acagtacgaa gaaacttcta 94980 gtgcaataac attcgcggat aaacgaatag ctgagtatac agattctaga gttaagaatg 95040 gtgtgattac tttaccactt tgggatgtga tggaaaagtt tggtaaagat ttgtgcccag 95100 gttgtgtacc attgtttgtt actattctaa ttgatgaaaa ggatcttaaa tgattatctc 95160 tccatttgct ttgtggtttg ttattggtgc agttgtcgcg atttacagcc tgattgatga 95220 tctgtatatc tctaaaaata aagatgttat tctttatgtt ataaacaaat atcgtccacc 95280 agaattagca gaatttgacg atgaaaagat gttcaaattc attatgtcag gtatgcttat 95340 catggacacc ttgttcgggc cagtagctac ttacttcttc ttccgtaaaa ctcgtaagat 95400 gaaaaagttt cgcgctgaaa tgcagagcaa gtaaagaact cgcgtaggac cgagttgcgt 95460 cacctgcaac tgttacggat aagggagtcg tgccccttag tgcggtaagt ggtgagttgg 95520 tcgctgacct caactttaac tggagatatc atgagcttta ttttattcat tgtagcagca 95580 tggtttatta ttggtttagg ctatgctatt gcagttattc gtcatctgga tgaatattct 95640 gctgaatggt ttattaagta cctacgttta gacgatcaag aacacgagtt cgacagtgct 95700 gaggaaagac aggctgtcca gtcaatgact cccaaagaat gcttgcttta catgcgtgta 95760 attggtttct ttggactgtt ggtagcgggg ccgctaggtc ttactattgc tccctacaaa 95820 gaagttgttg acgacattaa actatggaga accgctggat cgctacgtcg tgctagagaa 95880 ttaagtaaac aagagtgatg ataatcacct cctagcaaga ataataatga caaagcccag 95940 cctgcaattt tgcgactggg ctatttttgt aataaaattt cagttgctca gatgccacaa 96000 gtattgtata atatgtttat agattgaggc aaaaggataa tttatgagaa tcatttcaaa 96060 gttcaatgat gtatacgatt tgcagaatag tttatttgat ccagatcgtg tctgggagcg 96120 taaaactgaa gagctgctag taaaagtaac cgacgatgta gaaaagaata ttgttcataa 96180 tcgtcaggtg tttcgtgaag gttccttatc tttccgtggt gatttcgaat actcagtaaa 96240 tccgctgttt attgcaggtg aagtttactg gttacatgag ctgtcctgct ggcatccggc 96300 attcagtttc aaaacgttca acatcgatgc tgtatttgat aaaatggaag aaatgggatt 96360 acatgcccgt tcttacttac tagacaagaa tcgtggtgat gttcgtgaaa ccatgcgaga 96420 tatgttggtt gaagcaaaac ctaaagcaga acgtatctta tctgaactgc gcgtaccaat 96480 tgcatatgtg aaaggtatta agaagaacga taacgatgat gttcgtaact tcgtaattca 96540 gacaaacatt cgtttccacc agtcaggtat tccgtggcag gaaattgaaa gtaacttata 96600 ccgtttacac caagttctcg aacagtatat cttcggtgta ctaggaactg gtgagcctga 96660 tatgattacc gtatcagata aagacaggtt agcagcacat ggatttgata caaaaacttc 96720 tttcaggaat atggcaaggt aagtggtata tcctgggagc aggtctagcg ataggcatcg 96780 gattcggtgc ttatcactta gtaaataagg ttgagactct agcaggtgat cttgcagtag 96840 ctactaaaaa gattacgtcc ctagagactt cccttaataa tgtgaaagct gagagtgaac 96900 ttcgtgaaac tcgtatgaat cagtatttca caatgaataa tgtttcccaa gcagatctag 96960 acaagaaaat tagtcagctt gataaagcac ttagtcgtca agacattata tctgcaaagc 97020 ctggattggt tacattaatt gctaaaaagc agagtaagga gttcgaggaa agattagcat 97080 gtttaactgg aaacttggag tactgctcgc agccgcaatc acaatcacag gctgtgcaga 97140 agaaataaaa ccagagcctt cacatgagtt aaagcaagcc catgttgact ggcctaaagg 97200 acttcagccg tgtagctttg atttcaaatt cgaaaagaaa ttagctacaa atggcgaaga 97260 tggcgtagta gttgttgtac cttacaaaga ttggaatgca aaagcaaaat gcgaggaagc 97320 agtttactct tacatttcac gactgactgg tatggtttgc ttctatcgtc aagatttaca 97380 agaaaaacgt tgcttagttt actatccacc cattacaaat aggaaagatt aatgtcagta 97440 ttagtcggtt tacatggtga ggctggtgcg ggaaaagata ccgttgcaga attaatgatt 97500 gactggtgta atgacacgtt cccaacgtgt ttatcccgtc gttacagttt tgctaaaccc 97560 gtttacgaac ttgcatccgt aatcctcggg gtaactccag agtttctggg agagcgcagg 97620 ggaaaagaga ttgaccaatg gtttacggtc acacaatctc aattggagcg agctagagac 97680 gtatggttta agtatggtat cgataagttt gaggacttct cgtacgtttg gccgattttt 97740 gaaggaaaat accttgatcc tcagcagtta attgggcaag ctgatgatgc gctgtatagc 97800 gtatttatct ccccaagaaa agtgctacag cttgtaggta ctgagttagg aaggcagctg 97860 gtgcatgaac gcatttggct cataattctg gagcaatcca tcgataaaga cgacccagac 97920 gtcgcaatcg ttaccgatgt gcgttttccc aatgagggag agctaatcat ggaaaccaat 97980 actctagata tggattccat acttattaag gttgaatctg gtattactaa taagtatcgc 98040 atcgagacag atcatccgtc tgaaagcggt attcctgaaa aatacatttc tcataaattt 98100 gagaatacct tcaacggact tgatgcactt cgtactgatg tataccactt ctgtgactta 98160 gagctagaac cactagttgg ataattaagg atcactatga ctaataagaa agaagaaaag 98220 actaacctgt ttcacactgt tcgccaatct aacgaatata cctttttctt tgatgaagaa 98280 ttaggctcac cggatgagta tcgtgaccta tccatgatac tgatgcaggc tggtgaagat 98340 gatgaaatta atctgatgat taatgggccg ggtggttacg ttgatactgc tgcacaacta 98400 tctaacctaa tcgctaactg tcgtggaact gttattggac acctactcgg tcctagtgct 98460 tctgcttact gcacaatctt cctatcttgc cacggttggg ttgtacatcc acatgctacg 98520 cttatgggac atacgttctc tggcggattt tgcgagaaag gtcaagaaat caagaaagcc 98580 tatgaatcct acaaccagtt tgtagaagat atgatgctag atatttactt tccgttcttc 98640 tctgtcgatg aaatcgagga catggtaaaa gagaataaga atatctatct caacagcaaa 98700 gaaatcaata agcgtattga aattttggcg aattatcgta aagagcagtg tagtaaagcc 98760 caagccccgc atccagaaga acatggagaa gtgtaagtag ttttaagcca gagtttaacg 98820 actctggctt ttcttttgcc taaaattctt cttgacaacg acaaaaaatt gtgctccaga 98880 agtgatctta aaaaacacat tgtaatttta ctctagaatt agtataatgg tagtggttag 98940 aggaggttac aagtggaaaa gtttatacaa ctattaacga tactgctaca agaagcgaaa 99000 gatccagctt cgctactaaa acgtctgcta actatcttag ttgctgtcat tattttcttg 99060 tttgtaagta acactagcga ggtgatgtca tttttaaaga ctttctccac gtcttcggtt 99120 ttacaggatg ttcaaaccca aaggatagat aactttccta atgtagcacg agaaaagagt 99180 atggttcttt tctcccagac tggtgcggat gccgtttttg tagtaaagta taaaccagat 99240 gctataaatg attattcaaa cattattgca tgggaaagca atgcgcaatt ggatagggct 99300 gacttggccg ataaagcagt aaataagacg tctgaacttt atagacgtca cctggaaggc 99360 tttaactacg ctgcggattt aagtgtaaaa gtaaataaat atactgggtc aaatatacca 99420 gcatttaaga acattacttt taattacgtc tacacttgtc catatttcaa cctaaataat 99480 atctatgctg gatacatagg tattgcatgg aaggataaac ccgtagatac agctgattcg 99540 gaacaattta atgagtactt agcaaagctc tgttccccac aacggagatc attaggtaga 99600 tctatatgag ttttaaattt ggtaaaaata gcgaaaaaca attagcaact gttaagcccg 99660 agctacaaaa ggtagctcgt agggctttag aactatcccc atacgatttc acaatcgtac 99720 agggtattcg tacagtagca caaagtgccc aaaatattgc taatggtact tcattcttga 99780 aagaccctag caaaagtaag catgtaactg gagacgctat cgattttgca ccatatatta 99840 atggtaagat tgattggaaa gatctggaag cattttgggc tgttaagaag gcttttgaac 99900 aagccggtaa ggaacttggt atcaaacttc gttttggtgc cgactggaat agttctggtg 99960 attatcatga tgagatcgat cgtggtactt acgacggtgg tcacgtagaa ttagtctgat 100020 aataaatcaa ggcggggtaa ttcccgcctt ttaaagcaaa gggggcttta aaaacttaat 100080 gataggagaa agccatgttt gcagaactat tcaccatgat attactagga gtatggaaaa 100140 taagcctagt aattttcatc ttaatgataa ttctcactgt tgtcgcacta gttacccaaa 100200 atgatacatt aaagaaagtc gttcatgggc tagagtatat aattatgggc acattcggcg 100260 tttgcaaatg caactgccag aggaatacga aatattgttg gctttggatg gagttagaga 100320 actgcatcac agtagccctg gctgtctcat tcggtatgat tcttatggcc cttactctag 100380 cattgatacc actaatgtta gctggggagt cacggcttat ttcacccttt tctcccctat 100440 acttatgtat tcaatatatc caataactat gtacttagtt aggaagagat tcccacatca 100500 agcacactaa tataaaaaag tagttgactt tccgctgaaa tttttatata atatttgtat 100560 aaattgaatc agaggagtct cacaaatgtc agatcgttac tatactcaga tggctaacca 100620 ttacaatatg gctccgtatg aattgaacat cgcattacgt gacctcgatt caccagagcg 100680 tgcaaaactt gagaaaaagg cgggaattcg tatgtctagc aaaggcaaga aacttactcg 100740 tattgactta aataccatgc taatggaaga gcttggcgtg aatattgaag gtcagaagtt 100800 gcctctcaat gttcttgaaa cgatgctgga taaagtcaaa aagaaaacct ataagaaagt 100860 acaagtaccg gaaggcaggc taaaagcccc ttatcaggct gctgtatctg agtgcttagg 100920 tgctacctta gatcttagca ctgcaactgt taaagtgatg cgtgcgtttt tagaggcagt 100980 taataaattg tgattaccca agaaagattg aaggaacttc tagagtacaa tccagataca 101040 gggatattta tatggaagag ccgaaaagta gatagaagtg gttttaacaa gcaatttgca 101100 ggaaaaattg ctggcaatcc tcaaaataac ggttatatca atattactgt tgattccgaa 101160 gttcatgggg cacatagatt agcctttctt tacatggaag gctatatgcc tgcaagagta 101220 gaccacgaaa acagagttag gtctgataat aaatggctta acctacgaga agctacagtc 101280 tctcaaaatg gtgctaatgc gttaaagtat tcgaacaata gttctggata taagaacgta 101340 tattggacaa agaaaacatg tagatggcaa gtacaggtta tgaaagatgg taaatcctac 101400 tcgggaggat acttcacaaa cctcaaagat gctgtagaaa ctgccaatga gttaagattt 101460 gaattatttg gggaatttgc cctatatgaa ccatttaaag gagataaaca tgagtaaatt 101520 agtatatcta ttgaagggat ctacctgcaa tccgtgcaaa atgttcgagc ctgtatttga 101580 taaagtagtt caggactaca gtctagaagt tcataaagag actgataata cagaacttat 101640 gcagaaattt ggagttcgtc aggttcctgt agtagttcta gcagatcgtc tccctaatgg 101700 tcgtgtggaa gccaaccaca ttctgattgg tcgtcaactt cgtaaagaaa ctatgcatga 101760 cgcaatcaaa aacttcctgg aagataatcc agaggattaa tacccaaagc ctagtcttag 101820 ctgactaggc ttttttatta cttgctttct gctaaaaatt ttgatataat atatgtataa 101880 attgatggga ggagtatcac attgaattac gcatatctta taatacataa caacgtggtc 101940 tgtggtactc gtgctgtaga aatgggaatc acagaagaaa aatataagtc actgcctgaa 102000 aaggagcaag aatattatgt tgagcaagct gcttgggaat atgctgaggc ttatcccgaa 102060 gaacgtgaag gccgtgttac tattgttgtt actttggggc tggtgggctg tgataccgaa 102120 gttgatacag atctagaaac acttgaagaa tgggaagagt tggatattgc agaacaaaat 102180 gctatcatcc gtcaatcttt ctgggaagca gtagactgtc acgttgtctt tgagccaaac 102240 gatacagaag ctgaaaaaca cactaattgg atgactcgat aatgaaaaaa gaatttaacc 102300 tgcacgaaat gttagtagta ccagatgatg ttaacctgtt cttcgtagga gatattcacg 102360 gctgtaatga tatgctggaa gaggctctta aactagcagg gtataaagaa aagcgtgact 102420 atgttgtctg tgttggcgac ctgatcgacc gtggcccgca gaacttacag gttctagcca 102480 aattcttata taacccacgc ttccgtagcg ttcgtggcaa ccatgatcag tttatgattg 102540 ctggtgacta cgccaactgg atgtataatg gtggaatgtg ggcaatgaat gatctggata 102600 ccgatacaat taaaggtatc gcagaagata tggcagctaa aatgcctgta ttcatgaccg 102660 tactccatcg tggcaagaaa tacggtgttg ttcatggcgg aataccgttt cagtataaag 102720 atgctggatt cgatgtacat acacctaatt gggatgatat tattgatcat atcgctgcaa 102780 gtgaaaatga tcccaatgat catcctagct attacattga gccgtatctg tgggatcgtg 102840 atgtaattca ggaaattggt tttcatctgt ctaagcaagg tgcagagcat ccctatttcc 102900 agcgttatgc aggatttaaa gatgaattga ctgtggaagt accagaagtt gaaagtgtag 102960 atttcgtatt ccatggtcac actggtgttc cttacccaat tctgtacaag aaccgtgttt 103020 atctcgatac tggcggtgtt ttcaacgggc agttgacggt tgcgcaagtt aatgatgaag 103080 ccggtaaaat catgacattt accacagata aaaatgatag ctgtggtgta cagaggattc 103140 tataatggaa cgtgaagaac gttatgtagt tataaagcta tctgatattg agaaagccat 103200 agaattaggg catatcactc aaagtaactt tactactttg gagcatatct tgtccaaact 103260 atggtatagt cgagcacaac gtggtaaaga ggatcttaaa acagtcgtag tagagcataa 103320 ttggccagag tacgaagaag tctggaagat gctagagaat cgtgttgaat ttgaacaagc 103380 acgacaggaa gaggttgaca aaggctacca agaagcgaaa gagcaccatg aggtttatga 103440 tgaaatattc aaacttcgtg gtattagtag ttattgtcag ggttggaata aatatgcgga 103500 ggaagttctg taatgaagag attactacta ttatcagtac taatactagc tggatgtgat 103560 tcctacgacg ataatatgag atttactgca gaagatacaa aacacttatg tgatgtagct 103620 ggaggtgagc tagattattc cagtattcaa attggactca caaaaggtat attctacgat 103680 agttatacta ttacatccac ctgtgtaagg aataaataat gaaagttaaa ttctggagtg 103740 acctccactt agaccaccca gggattcttc gttttcgccc ctggtttaag aacatgaaag 103800 agcacgatga gtatatccta gatactttgt ccaagcacat tggcaagcgt acaaaacttg 103860 tgctgctagg agatagtgcc gtgggggcga gaagggttgg ccaagttgaa gtcggttatc 103920 cacaacgtgc ctaaccaact aattatggga aaccacgatg ctgagcgtca gggtctgcat 103980 attaaagatc ttgtagacgt ttatgatgat attcaggcta taggtaagca aacaggtggt 104040 ggcaagaagt tttggctgtc acattgtcca ttgcacgaag cagaacttcg tggtaggcta 104100 aacattcacg gacatgtgca cactaattct gtgcgtgacc cacgttatat caacgtctct 104160 tttgagatga gcaagactcc tattcctcta gaggatattg tgtctggcaa attcacaagc 104220 tatgataaag tggttaactg cactactggt gaagtactcc aaatcgatga agatgtggca 104280 gcggtattga ggggagatat caatgtttga ttttgatggc atggctcgtg agatatttat 104340 agcaataggg atggtagttc ttatctcttt ctttacgggc ctaggcttag gccttttatt 104400 ttaaaaactt catttgctaa aatgcttagt tttctgtata attactttat aaattgatga 104460 gaaggaatca aaatgcaaaa gattgataaa gcacttttaa tcgcaacagc agataagttt 104520 gagcaagtta aatctacttt taaggcagtc tttcagtcct atgttcaaga caaatcgaac 104580 ccgatttctg agcgtttgat gatgtgggaa tgttttgctt gcaatgctct gctaatttcc 104640 gattataagg gagaagtatc tgaggaattg catgagatct ttattaatga ggatactcca 104700 cgctatcagt tagtgaactt ccaagatcta gctgagcaag ttattcctga tgatctttgg 104760 gacaagtatt acggtgatcc tgaagaagat ggtatggaac ctgagaagtg cattgaactg 104820 atctgtaaag atcaccctga aattgctgag aaatttgagt tagtatttgc ttctgagttc 104880 tcaggtttcg ttaacgattg gtaagtaaat aaaaaattca gttgctttta gctttaaaat 104940 tctgtataat aagttcataa attaatgaga gaggaaattc taatggaaaa catgactaaa 105000 actgaaatgg ctaacgttct ggcaattctc ctcgatatgc agggttttga aggtcagctc 105060 atgaagatgt ctattccggc aatgaaaaag atgtatgatt ctctgaacaa gaatgctatg 105120 gcattcaacc ttgcgaaaca agaagcacgt tttgcgaaag agcatcaggc aactgccgaa 105180 cgtcgtgcag catcttttga gcgtgaagtt aagcaactga aaggtaagaa ataatgacta 105240 atggaaaatt cttacttgta ctagggctga atgccctagt ctccctagct atcacactcg 105300 gtgttagtga cctcatgctt aatcagcatg agaagaaaga agctcttgaa caagtgtaca 105360 ttagcccgga aatcaaagaa caagcaaaac gtaataaaat gctctcagaa gcaaaccttg 105420 tgcttaatca agcagagttt gatatagctc tctcaaaata ttccggtgct gaagtaaaag 105480 atgcacacgt tatgaaagac ttggaagaaa taaagcatca aattgaagag gccaaaagat 105540 tttgtaaatc aattgaccaa tctttagaaa aaatcacgtt ttatagaact ataagcccat 105600 atattgaatg tggcaacgga gtatttaaat gattacagaa attttaattg gattgctggt 105660 tctcacgact ttagcagcaa ttggcggaat cattggcatt attaatgcta ataaaaatat 105720 ggataggatg cgcctcacca atgatgggct agttaaacgt ctcgttgaac aggaagaaga 105780 tatcgaatta gcacaaagac gctcagatct tcttaaagaa aagctaacga atattgaaac 105840 tcttgttggt aacactaaac ttcctattaa agtcctgcgc gctcaggtaa tcacggagat 105900 taagaaatga tgttaattat cttagctttt tatctggtta ttgttggtgt tctggttact 105960 aagtatcata cctggacccc gaagaacgtt ggtaaagtag cactgtttgt tctgccagtt 106020 cctatcatca tcgttgcaat gctgttcacg atgttggccg gtaaagtgac aaaaactgac 106080 gttaaacgca ttgtagacga actacagcaa tcttctgaca tgatcgagga tattctgaaa 106140 gatgaagctt aattttagtg aattaaccct gctggacgag tttctaggtt ttgttactga 106200 acgtgggttt attaatgtag atcctcagac actagaagtc ttgcaatcca tccagaataa 106260 aactaaaaca gatctggaag acttatggcg accactttct gagctaactc ctcttaatat 106320 gaaagtgatt gttaaaaata ttgaaactgg tgaagaacgt gagatggttc gtaaagaact 106380 ggctaatagt tattctccag agtccctagt gatgcatcat gacgatgaac cagaaacact 106440 ttggacagcc cattatattt ggcgtctgcc ttgattgatt taacaactgt tacggcaatc 106500 ctaatcttcg gtttagtggt tgccgctttc ataatcgttg agcaggccaa agtaatacgg 106560 agacttaaaa acagatgatt aatttttata accctaactt gcagaaagca acgtgggatt 106620 actttataat tctaccagct tttatgttaa gttgtgcaat cattaacatt atgtatcaag 106680 aagctggttg gacagtccaa gatttccaca tttcacaagt tcttcctttc atgtatgaag 106740 gcgttatcgc agcaataaat ggggttcgta aatgacagca ctgcaaagat tacagaatta 106800 ctggtttgat ggccaattta atgatttatt catccagttg tttgtcgaaa cgaacggacg 106860 ttttaattac cgcttcttta atactttcca tgaaacgaag ttctcgcata cagagattaa 106920 cgcagcgatt caagatctca ccggatctaa ggtaattcct tatcgtgatg tagattttgc 106980 acctgactgt tttggttttg agctgttcaa gaaagcgtat aagttcggta agtttgaaaa 107040 tgcacgacat tgggttcatg atttctggta taataccgat atcgttccta gccgtgtact 107100 gatcctcaac tggattgcta aacaacatcc gccgaaggca caatcggcat tccttccaac 107160 tgatacagga aatctttacc atgacacaaa agaaaaatcc agtgctagag cagatgaagc 107220 agttggagaa gcagattgag gaaggaagcg ttgatgggca gtcaattgtc aatcatgctt 107280 tcgatcttac agtaggatgt gctaatccgc tggtggcggg tgaaacagca gatattcttg 107340 gacttttaat ggcagttggc cagttaattg agattaccca agaagagtta gacaaggatg 107400 aagaagtaac tattcatgat gttcttggtg ctctagatct gattgttaat gcttatgcct 107460 gcaaacgccg ccactcaatt gatgaatatc tagtttctcg tgagattact gaatctcttc 107520 tggaaactgc tgatatggcc ttaaacggca agcaacttca ctaaggaaat aattatgcaa 107580 tttaatgtta tatctgcttt tccagggtgt ggtaagactt actttactaa gtgttttcca 107640 cgtgtactag attcggattc ttctaaattc ccgaaggata actttccagc gaactatatt 107700 gagcacattt cacaagctat agaagagggt aaactgatct tagtttctag ccataaagaa 107760 gtacgagatg ctcttcatgc tgctggaata gattttattc tgctttatcc agaacgagag 107820 ctaaaagaag aatatttagc taggtacaaa gaaagaggaa gtcctaatag ctttatctct 107880 ttacttgata gtaaatggga cgaatggata acctcctgtg aatgtgaccc tgcgatctct 107940 atccctatgg aatcaggtga atttataacg gatttagtga gcattgacgc tacctaattt 108000 ctagaggcca ggacttaatt gttctggcct ttcgtgtata ctatgagaaa taaaataaac 108060 gaattacttt atgaggaagc attacaattc cccataaata gattcactaa atcagatgga 108120 tctattaata gaactaagct aaaacaactt catccagact tccagcaaga cgcacttaat 108180 cttattttta ttaggagagc tgtagaagca catggtaagt tctttgggta tgagagagtt 108240 aagtatagaa ctatgcaaca gcaggttgag atctattgcc cagatcacga tggttattac 108300 tggcaaacgg ctagatctca cttagaaggg catggttgcc gattatgtgc acataaagtt 108360 gtgacacgct taacagaata tggagtgtac accgtaccag cacctttcca caaatttgta 108420 gttgacggtg agcacataat ttggtataat aaagatcaaa agttaaaaca ggagttaaag 108480 aaagatggag tacgtagtct ggtcaaaccc cttagctcta ctctcccaca aactgttcaa 108540 gacgacgtgg ggaaggcatg agataactgt taatgagaat ccacttaatc atcaacatcc 108600 tttagtccca attctctaca agattactat aaatggtaca gaatacctat tagatgattt 108660 cggtaaagag tttgtacaag aagccataga cgacaataac gtctctatgg gcgaattaat 108720 taatagagca tctaattttg ataagagttg gatgttaatt tggagtaact aatggaaatt 108780 gtagcaagcg tcttagtagt tatgttattc atagtaatac tcgtatcttt cgttattatg 108840 cgtagagcca gagaattagc agaggatcta acaaagttac gtggagaggt ggctaatctc 108900 aaacaacagc gtgaagctct gaagttattc gtggcccaag gtagcctaga acatacagca 108960 gaagacttca tagtatatct taaacgttac atgggaatca aataatggaa acattacttc 109020 ttgtactact aggcggctca ctcttaacta ttctaggctt gataattata tgcagtcgtc 109080 ttaatgttgt taagaatcta gaattaaata gtgagaatga aattcttaac cgagaaatta 109140 aacaacataa tatcgcagct cgtaagctgt tggataaact ggagaataga taatgtttac 109200 acaacaaacc attaaagtag ttgcagatca tgaactcgac acatgggatt acagctttaa 109260 agatctgact gctacagtac agtttaaatc tatgcaactg tcgtttttgc acattgatct 109320 tcaggcttgc aaagagtttc aggccaaact catgaatgct cgtaagatct ttggcacagc 109380 tcgcatcccg gcagataatc tgattaattc actaatcgat gctggataca aactcgtgaa 109440 gactgaggtg aaccgcccga gtactccagt gactattaga gatccgtggc agtcgggacc 109500 aatcgcaatg ctgaataact ccgataaaag attaatgtcg gatatgcaca atgttccgtg 109560 tggtggcgtg gtaaataaca cacaatcata taagtttggg gaagattgca gtccttctgt 109620 acaattgaaa catactcaag aagctataga gtctgacatg cgaagcgtag caccttctga 109680 tgctggagtt tctaggaact catatactat tcatctgagc acgccatcta gtaagaatga 109740 gagctttgct gatgcagtta tggaagctct tgttgatctg actaatagta agggttttaa 109800 ttaatggaac tagttactga atttactata ggccactgca aggtggcttt tctcaatgaa 109860 aagtctttta ataacgaaga aactattggg atctttgaaa ttaaagagga tgagtatgaa 109920 cgtgtggaaa ggctagcgta tttattcttt gctgatggtc atttaatgaa tgatctcaaa 109980 gagctagtat ttagactgcc aaaagatata cctttcacag ctagaaacat gtctcatatt 110040 gtcgctcaac taaatctaat atggattaaa ttagatcccg ctatagatag agtcataacg 110100 ttaaaagaaa gaccagttaa aaaatttaca ggtgatcaag cctgtgatat ctagtagtaa 110160 agcacaagcc tctacggagg ctttaaaata aaaagcaggt catctgacct taaaaaggag 110220 atataaataa tgtataactt taaagataaa ctaagacaga atgctggtaa aacaaaacct 110280 aaggagaaca ataatgttcc taatgctaaa gtacttccat ttcctgaact aactcaagaa 110340 gaaaaagata aactagcatt tatgttcgat gcacttatag gattaagtgg acgactgtac 110400 tccactctta gtattaatga gaaacgctta gtagagatgt tctgcaaacg ttatccagtt 110460 acttcaaagg gtaatgcaca taaagaaggt gaaaaagcat tcacagtctt aaaaatcagt 110520 gcatatgcac atcgtttctt agtttatgga ttacagatgg aatacttcac catcccacca 110580 tttaagtcat tccatatctt tgaagatgaa cgaatgaata ttgatgatct ttatggagat 110640 acgtacaaat attcaggaag tccattccat gatgcattaa ggagctaccc tactagttac 110700 ctaccagtta gagcatcaca aggtaaagct agcatctttg ctatatactc aggatctaaa 110760 gcatctgatc tagagactta tatatttaaa gagcgtaaat ctgtgcttcc agatgaagca 110820 ttatataata atccccttct tccagaaaaa gaaccagaga aaattgatgt aactccacca 110880 atcccatctg agtctcaata tttaatctat gagaaacaag ttaagttatc tctagacatt 110940 gggttagtta caccgcttgc tgcagcattc atcaatagat atctagaagt tcctgacgag 111000 ttccacacac gttataacac gaatattcag atcacagatg agttaattgc acttgcaaaa 111060 gatacaccta ctaacactga ggcgtaccaa aaagcatacc aagagttact gtaagatcct 111120 gcatgtctcg gtgcacaata cctgtaccga gactcaaata caaactctcg ttgacagata 111180 cttaaatgta tgttatcata actctatgag ttaatagaat taaccattta gagttgccac 111240 acagaaggtg tggtggttca ggaatcttaa attactgact tgtagcgtag gttacggagc 111300 gacaggacaa gtcaacaggt aatttaataa agattactag aaacatcacg agaccttctg 111360 gcgggggcaa agctctatat gagaaccctg gggggatctt atatttaaaa taaaagtaaa 111420 acttttgtac acataattta cattcaagat gatacactcg gttgccgctc atctgctagg 111480 atggcacaac actttctagt agactcctaa gtttctagga aaactctcgt caactcaggc 111540 ggttttcgtg ttcctagaaa ctctagcaat aggcggttta atacagggcg gtttaataca 111600 ggcgttt 111607                          SEQUENCE LISTING <110> Optipharm.CO., LTD   <120> Novel Salmonella specific bacteriophage SH9 and antibacterial        composition comprising the same <130> OTP 1.33 P-1 <150> KR 10-2016-0179216 <151> 2016-12-26 <160> 1 <170> PatentIn version 3.2 <210> 1 <211> 111607 <212> DNA <213> bacteriophage SH9 <400> 1 tatttctagg agacattttt ccaaaacaca tttctggaaa ttgctatcgg atttgcacac 60 tgtgtttata gaaatttatg gtcggatttg cacattgata ggagtctcca aagtttttag 120 gaacactgaa aatagcagca ttatttcggg ccgaaggccc atgattgcct gagtttttag 180 agccacagga attttcactt gtcaagcgat tttgcctcat tttgctaata aaatttcgct 240 ttatctccac taatctcaat tctagccaaa acatcccaca ttatccccaa aaacgcagca 300 aaccggtgct gaagcaccac taccgctgct ccttccgtag ttcctcccaa aatctcatcg 360 gattcgcact tccccccaaa cccacacaat cacacaccct ccaatcgaaa tcacaaaaat 420 cttgtcggat tcgcactact cattcctaga aatttcgtgt cgtatttgca catatacggc 480 tggatttgtg cgcagcacga gtctgcccga gtttttagga aaattcattg tcggattcgc 540 acactataaa aatatttccc atcggatttg cacactacca aaaattgtcg gattcgcaca 600 tacccgccag cacgtaatcc atggaggcaa atgtcggatt tgcacaaaag cgcagggcaa 660 aatgaaaatg ccctgcctgc cgaaatgcga atgataatga gattgattct catttgaaaa 720 gttgaatgcg aatgataatg attcgcgttt agaaagtgga atgagaatgg ttatcattta 780 cagaattgaa tgcgaatgag ttgcataatg ataatgattc gcatttagaa agggaagtga 840 gaaacactgc tatttaagtt atccacaggt ttattaacag catgatttga cttgacaaaa 900 tcctagcgat tttgaggcgt tatcaacaga cttattaaca ggttattcta ctgtataaat 960 atacagtata ttccagcagg agaataatct aatatagcct ttaaacgctc tgtaacgcat 1020 tctaacgcgt ttatttttgg aggaggtact ttaataaggg gaaatttgtt tgcaggcaga 1080 aaaggcgggg cgcaactatc ggccccgtta ttattagtct gctatgtatt ccttctcgta 1140 gtcgtcaaag atctggactg tttcggctgg cgtatatccg tatgaatcaa aatgcgtaca 1200 cgttgagact gaattgatcc acgcccctag cgccgccgta ctagctccgc ttttccgcat 1260 ttctaaaaat tgcgcgtatt gcccatcagt taaatgatgt tgcataatat cggtttcttt 1320 acgttctaca tatcgtaaaa ttcttacagc catgccattg ctcctagaat gagaatgatt 1380 atcaatatca ttgtgaagcc attaccaaat cgtaaacgct gggaatgttt cccagcggta 1440 ttattagatt tgtgactttg cgttttacgc caccagctat tcacggtaga cgctccaacc 1500 gtctagcttc cattcctcac ccttgcgaat attgaaaaac caatcattaa aattgtaacc 1560 atcttctgac atgatcacgc cgttttcttt catgtacaaa aattcatcgc tagtaaaata 1620 ttgattacta actttgaaac cttgtttcat tttttctagt gcttcatctt tagtcattgc 1680 gccacctttc gaaacgctgt ttcaatactt tcagcggttg attgaatata tgcgcctttc 1740 ataatcaggc gctccgtaca tactttgcgg aaaatgtcaa taaattcatt ttcgttttcc 1800 gctattaacg cttcccccat aacctgaacg tcacaagacc caaaagttaa aaattgaaac 1860 gctagttcac gggcgacaac ggcagaaatt ttataatgtg ctttgccctc atagatatta 1920 actacgcctt tataaactgg tgaaagctga tttaaatagt ttgctaataa tgccggacgg 1980 caattagtaa taaagcgatt gccctcataa attaccatta aaagatcctc gcgtcacgga 2040 taatcataat cactagcgtg atcaacattg cagcactaga cacgccacaa acaaaagtta 2100 acattttaac aattgccctt tccattataa taccccacga aatgcagcgt aaaaggtggc 2160 aaagtgttta atcgtttcgg ctaccaaatt gccacgcttg tcaaatttct tttctgtaac 2220 acggaaagcg tttttgttgc gtgctacata ttcgatttta caatctggac gctcgaacgt 2280 gttaacatcc accagcttaa aacctttgga acgggcaagg gaaacattct ttaacatttt 2340 tattcccctt taccagtcat acagcctaac aagtaagaaa accccgcgat acctgctcca 2400 ataataacca cgtgaacgcc gaaagctatt acaaacagcg ataaaatagc gcccaagata 2460 ctagccagaa tagatcgaaa aataaccatt ttattagctc ctagaaatta ggggataata 2520 tatccccttt aataattaca ttaaaacaat ttttgccgct tgctttttgg tgcaatggtt 2580 agctttcaat tcatccagca aaaatccggc ggtttgctgg tcaatcttgt cgttgtcgta 2640 aagctcctga atggtaacat aagttttacc gatcccaaat tcagaaacca taacgctgac 2700 aatagcggga acggataccc attccatacc gttaaaaatt gcagaagatg atgaatgcac 2760 cattttaacg cctcctagaa atcagggaaa attttcaatc caccgtttcg gtgtaaaggc 2820 attataacaa aaaaggccgc cgattaaggc gacctttaca aaactttaca ttacatatat 2880 ctgattattg catcgccgta aagctcgctt tcagatcgta acacttgctc gaaacgctca 2940 tattctagcg ccctgttttc ttttgggaaa tagcaagcgt tttctagtgc cttttgataa 3000 acatgcaatt cattatcaaa acgctggacg atccccaatt ctagcaaggc tttatatact 3060 tcatcctcac catgaccgcc gttaacaaat tgataacttt tcgtgaattg ccagtggtac 3120 gccgttgcgc ccacgtctgg attttctacc agattggaat cgtcacactc gcaatcatta 3180 gcagactcat cacaattctg acagatttca tgattgctaa aactggaggc gttttccatt 3240 gccatttcat gcaacggcgt ttcaatatcg ccagcgaaaa aatgttctac acttgcccat 3300 tgttcgccgt gggtattagt gtaacagtac actagtgcaa tgatcggatg atcaatatgt 3360 gatccggtta ctacgtcgcc atactttgac attaattagt ctcctattaa ttagcaggga 3420 aatatagaga atttgctaca tcgtaaaatc ctttattcaa gaaaattacg tcatcacgga 3480 ctatttcatc cattccataa tccagcaaag tttcatctac aaaaatacat agccagccat 3540 aaccaggatc aaagaaagca tgataattat tcgcgctttc tacatgttcc cataaaaagg 3600 gacgcccttg cttttcccat ataggaatat tgctttgatc gtctgaataa ttaacgtgtg 3660 ggctattcgg cattttttcg gctaattgtg ctaacatgat ttgattcagt tgcataataa 3720 ttagtctcct aacaattaac agggaaatag taaagcgggg aataatcccc gctagtaatt 3780 attattctgt ttcggtgttt tgggtaaagg tagaaatgtc aatacctttt accatttcat 3840 cgatcatttc agcaatattg aaactctgta ccatttccaa cattgccgct tccattgccg 3900 cgcctgctac cgctttcgct ttctggttag tcggggcgta ctggtcaacc gctacactca 3960 gcaggttagc gatcactaca cgaccaatcg gcgtatcaat ataaccttta atcataaacg 4020 gcgctttttt cgccgctact tttgtgattt gagtcagtgc aattttaccc gcttccagtt 4080 tagcagcgtt aacaacggcg gatttattag cggcaacgat agaagatact ttagacataa 4140 ttttccttt attttcagta gtttgcgcat ttgcgcttga agtttgagat tgtacagcct 4200 gtttggacac attgtcaagc gggagaagtg taaatccagc gtttgcaacg tttgattcat 4260 tcccatactc taaataatca ttaggaatca acatgttacc cgtagcgcgt tcaaaacaca 4320 atacgccagt tgtttcgatt gcgtcactat cagcacgttt aatggcgctt gtcaattcgc 4380 cccaaatact agatagtaaa ccttctacat cgctggaagt aataccattt acgcgagtag 4440 cgaaaaccat ttctttttct tgaacattgt aaccacgggc aatatagctt ttttcgtcgt 4500 gacctacaat gataaaatca atatcgcttc cccgctgatt tcgcatgatg tgatagtcac 4560 ggttgtaaag cactagaacg cgggtagggt ctagtaattg cgtgaaatca cttaaacaat 4620 aatgagtatc attattatct aaaatatcat cgtgaatctt ttcaaccatc gccgccgtat 4680 gttgtaggct tgctggattt gatccatcta gcaggttgcg caaactaccc ctaataatat 4740 tggtgtttgc tggtactgca attcttacag tagacatttc tttgtctccc tcgttaatcg 4800 gtggaaagat aataagcgtt ttctctttat aggggcaacg tttattttac ctttttattt 4860 tccttgctca attgctaaaa gtgtgctaat cgcgcgttta ctcatataac gctatataac 4920 gcttattgct ggctttactt taataaggcc accagcaaga aaacgccttg tagcgcgttc 4980 tagatgcctc aaaattgatt gcagatgtta gtgctaccta tttaccctgt atatttatac 5040 agtagaataa cctgtggata actctgtgca aaagtgctgt aaactcctct cattgtgtca 5100 agtcaaaaag tgctgttaat aaaactgtgg ataacttaaa tgatagtggt tctcactaac 5160 tgaaaaactc attaggtagc ctgctaatga ttttgatata atataataga gattaaatta 5220 ataacatgag gaaacaatta gatgactaat tataaagata ttgattatga gactgtaaat 5280 aatttatttt attatgatga aacatcggaa acatttctaa ggtggaaagt ttcacgcggt 5340 aataaaaagg caggtgatat tgccggatgt gatagtaccg gtggttacta tcgcgtttct 5400 attaataact ctgattacac ggtacatcgt atcatatggg tactatttca tggtgaaata 5460 gatagcaacc taacaatcga ccatattgat cgtaacaggc gaaataataa cattaaaaac 5520 ttatgccttg ctactcaatt gcagaatatt agtaaccgtg gttacaaatt gcgtgctaat 5580 ggtttgccta ctaatattta tcatgataag aaacgcccac gtgatagttt aggtcgtgat 5640 tatttgcgtg ctcaaattac aaatcctatt actaagaaaa agattagtaa gtgtggttat 5700 gatttagata cgttattatt atggttagaa ggggaaagag aagaatttaa aaagtcaatt 5760 aattaattta ttaaattaag tcatttggtc ttgattttta aaagtcaaga ccttttctaa 5820 ctttatttca attttgcggt tgttaaatat ttgttaagtc caatttaagg cgtctaaatc 5880 gctctctacg gcgttctaat ttctagctag gcaagtgcat tgccactatg ccgatctcct 5940 caccacgagg attttagatt ttgtcaagta ccctagagca aatataaatg aaaaaattac 6000 ttgctaaaat ggttgttttg gtgttattcg cgcgcccgtt tcattaaatt tagtgcgccg 6060 ctggacgttt gcgaatagtg gttttcttta cgtttcttta caaaaataag gttgcgccct 6120 tctcggtatt ctcctattat ttatcacgta gggcggcaat gacgaactac acggcgggga 6180 agtttaccgc catgctcttt aaaaactagg gaacatctta aagcctatcg gcggtaaact 6240 tatacattat aaggaatcga cgataatgat caaatcaaaa aacgctttag tgattagtgc 6300 gaaacctgct agcgcattag atagcagaac aaaaaatttg ttgacagtag cgcaacgatc 6360 tgtaaaatac agattcaagc aatacaagaa aggcaagcaa caagggctgg aaatggtgtg 6420 gcgtaatata atgattgatt tatataactc acatcaaatc gaaaaaagtt taaaaaacgc 6480 ttgacacgct agccgatagg ttttaagatg ttccccgtag taactgctct ttaaaaattt 6540 ggataacggt aatgtttagg ctaactccta gcggattata atctaatagc gtttcaaagt 6600 atcgaggcgc tattgtggaa taatccccaa taaaccggag tattaaaaat gcaaaacgtt 6660 attaccgcgc cgaaagtagg tcaatctgtt ttcattcctt ttgtaactaa aactgatgaa 6720 ttaaccggaa aagccgaacg tatcaaaggt gcggcgcttg atcctttcga taaaatcgaa 6780 gcggtttacg cggaaacgga acgtagcaac aacggcaagc cgatttatac cgtccgtgtt 6840 aaatctggcg acgttgtaaa ggtcattcag aaagatgata aatggcaagc tatcgcctaa 6900 tagcttatta acccgcgcct ttaatcggcg taaataaata aggctagcgc ctcatgttag 6960 attttcgata tattaaccat gtagtgtgaa caatgttatg gcacacaatt taaacttata 7020 tgattagtgt tacttttata ttgccctgaa atatgggcaa tataacgatt aacattagtt 7080 aatcataatc ccttaattaa taattgaacg gagtatttat tatgtctaac gttaaaaccg 7140 aaaaaaccgc aaaattctct tggaacgaag aaaacaccga aaaggctgtt tctatgtatt 7200 ctgaaatggt cgctaaaagc ggaattgaat ttgctaacag tgacggcttg aaagaaatcg 7260 ccgccgccgt tggtgctgct agtccggtgt ctgtacgttc taaattgacc agtgcaaaag 7320 cctaccagaa aagcgataaa ccgcgtaaag ttggcggcgg gtctagtgtc cgtaaagctc 7380 actatgtgcg cgttatggct aaacatgcca ttgacgctgg cgttattaaa gacgccgacg 7440 atctggcaag tctggaaagt gcaaaactgg aaacgctgga cgtcgtagcg caattgttag 7500 gcgtagcgga ggaagtaaaa caggccgcag gtgaataaat tttaactagc ggggaatatt 7560 ccccgctata atttcccttt gaccggagtt attaaaatga ttttctttcc tagtgaagcg 7620 tttattttag gtttatttat agttgccgct atatttatat ttaattacgg caaatatata 7680 atctctatta tcggcattaa ttcagcttat gccgctgata tgtttaaacg tcaagctaat 7740 aaacaaaaat atctggcgat tatattttta acgctggcta ttagttgcgg gtttagttct 7800 agtcttatgc caattctaga ataatattgc aaattataac gccttttaaa ttgtcctcac 7860 ttcaggcaag gcgttatatt tggcaatatt gccagtatta atatttatcc ctttatagga 7920 gttgatttag ttatgaatat ttccgcagaa aaacaagctc aaattttgaa aatggccgct 7980 gattttaatt tctatggtaa gcgtttacgc gctactaaat tggaagtttg cgacgatatt 8040 tcaaaagctg tttatgatac gccgaaacat tcgaccgcaa tttgtgactg gctggaagca 8100 aataagccag cgaaaccgaa agcggcaaaa gctgaaaagg cgattaaaaa tgatgatcgc 8160 cccgaagcgg cgggaattgt ttctagtacc gttgaacaat gggaagttaa atccggtcgc 8220 cgttttattc taacgtcgat tcaaaataat actttcccgc ataaaaactt tttagcaagt 8280 ctggaaaaat tcgctgaata ttgcggcgct gaattgctga ttagtaaatt catttataat 8340 aaaaatggat tccagaacgg ggaaggggca aacggtatta aatacgatcc cgcatttgat 8400 aaatatgttt gtgataaaaa cgtattttta aataaccgcc gctttgcttt catggctgaa 8460 attaatgttt taccaacggc agattatccg cttacaggat tcgcagaaac cgccacggct 8520 ttaaatattg aaggtctgac aattggtgcg gcaaaaatta ccgctgaaag tgtaccagcg 8580 ttaaaaggtg aagtagtgcg ccgtatgtat tcaaccggaa cggctacgct taaaaactac 8640 attcagcaga aagcaggtca aaaagctgag gctttacata attacggcgc tttgctggta 8700 gaatttgacg acgacggaga attttattgc cgccaacttg aaacaatgga tgaaagcgga 8760 atgttctatg atttgaatgt ttgcgctatg cctaccggat gttttgaaac gtccgatcat 8820 gttctaggct tgcaatatgg cgatattcac gccgaaaaat tagatgatga atgcgccgtt 8880 gcatcatggg ctagtgaaaa cagcttgctt gatattctta aaccgaaata tcaatttatt 8940 catgacgtgc atgattttac atcgcgtaac catcacaacc gcgctagtgg tgtattcctt 9000 gcgaaacaat acgccgccgg acgtgataaa gttattgatg atcttatcga tacgggtcgc 9060 gtattagagt ctatggagcg tgatttctct caaacaatca ttgtggaatc taaccatgat 9120 ttagctttat cccgctggtt agatgatcgt aacgctaaca ttaaagacga tcccgcaaac 9180 gcggaattgt atcaccgtct aaacgctgct atatacgccg ctattgctga aaaggatgat 9240 acctttaacg tgctagatta cgccttgcgt agtgtcgcag gttgcgaatt taacgctatt 9300 ttcctgacta ctgaccagtc gtttaaaatc gctggcattg aatgcggcgt acatggtcac 9360 aatggcatta atggcagtcg gggcaatcct aagcagttta aaaagttggg acgcctcaac 9420 actggccata ctcacacggc tagcatatac ggcggcgttt ataccgctgg cgtggcgggg 9480 tcgcttgata tgggctacaa cgtgggcgcg tcaagctgga cgcaaacgca tttaattacc 9540 tatgcaaacg ggcaacgtac tttgattgat ttcaaaaacg gtaaattctt tgcataatta 9600 aatttaatcg gcggttgaaa tataccgcca ttaataaaag gtttattaaa tgaatattgt 9660 attacctaaa acactggata aaaagcagta tataacgccg gatgatatgg aatgcggcaa 9720 ggtataccaa ttcgattata tgttggctag tccgacgaat acaggttgta ttattgcagt 9780 tgatacccgt gataatgacg tattaacgtt taatgattat tctgacttta tggaatggtt 9840 agatgatcaa agtattacta ataacggcga attaacgccg tatcaattaa caattgcaat 9900 taaataaatt aatcccgtaa ttaaataatc tggagtcata aacatgaaaa accaaaacat 9960 tccttttgat cgtgctactt cttccatcgt tctagtttat tctaatggcg aacgttatca 10020 cgtcgaggcg gggcaagtta atttcgattt gctgaattat aacgacgctt tgcaggttac 10080 tacctttgct tatgataccg gcaaagtggc aagccataca aaagcaaaag gcgtttacgc 10140 tgatactttg acagtgcaaa caattattat tgatgcgctt aaatccggtc tagcgtttgc 10200 agttgttaag ccgtgtccgg tctgcaatcc agaagctagc gcaatggttt acacttgcgc 10260 gggaattcgt tctagcgtac atggtgaaga tcttagcttt atcggtgacg cgctagcgta 10320 tggcctcact aactaatagt taattgaaca ttgccagcta atctaatata attagctggc 10380 aagattgaat taacctaaat cccttaaatg gagaatgtaa caaaatgaat aaagtcaata 10440 tgaatattac ccgcgctttc ccgcatattt cccgcgttat gatttgggat ttagacggaa 10500 cgattataaa ttctttccat cgtgtagcgc cttgctttga tgataacggg aatttagatt 10560 taaataagta ttcccgcgaa gcctgcaaac atgatttaat tatgcaagat tctttattgc 10620 cattggttga atatatgcgc caatgtatga atgatgcaaa tactttaaat attatttgta 10680 ccgcgcgctt aatgtctaaa agcgattatt tttatttacg caaacaaggc ttgcgagggc 10740 gcggaaatag taatatccgc gtattttccc gcgatacact gcacaaatac tttgaagcgg 10800 ataaggttag cgaaatatac cacagcaaag atgctgtata taaatcttat tactttgaac 10860 tgtttaaaca gctatatcct aacgctgatt tcacgatgat tgatgatcat aaaggtgtat 10920 tatcggcggc ggcaagtgct ggatttaaaa cgctggacgc tcaagccgtt aatgatatcc 10980 tatcaatcgg cgtaacattg atcggtgaaa cctttattga tgaatcgctt gatgatgata 11040 acgattatca attcctagcc gaacgtttaa aaatgtgttg ggaaggtatg accgaggaag 11100 aacgcgcgga atatagttgc agcccgcaac aattcattga taaattaaaa gttgcataac 11160 aattaaaaat aaaatagtca cactatgtta tcatcacata gtgtgactat tagcttgcta 11220 agtaccgggg cggtaatgag actcattctc atctagggaa cctctgggca caccttcatg 11280 tgtaatctta tgaaatttcg agaattggta attagcgcta acttaggaag aacaccggtc 11340 tactggcttg tctcctgatt ctgacatacg aaacctcctc tgcgacgccc gagatccatc 11400 cgaccactct cgctcaaact ccgcacgttt ctccgcaaac ttccgctccc tttccgaaag 11460 ctcccgcgat actcccgcaa cgtattcacg atgcttgtta atagccccga gtgttccgaa 11520 gattcccgca agtaccccga taatgaagat agctacaaaa ctaaacatta cttgtctccc 11580 cgcagcttac gttcgatacg agctaagtgg ttatctaaga tgcactgccc tataattacc 11640 acaactagta aagtaataat tactacttcc attataacca ccccgccata gctgctaacc 11700 aaaagagtac aaagataaag aataacacag tcccgaatcc gaaactattt atccaacttt 11760 cgtacacttc cccgcgtctg ttctttcttc tgttgtcatc tgacatctct ttgcttctcc 11820 tatagtcttg gtatttggaa taccctagca attgcacgaa ccattacgac tgtcctgatt 11880 agatccatta cttcgtcgaa gccggattct tctagtaaga aggatgttcc catcacgtct 11940 tctattgtat cttctatgac gtcttgaatg atttcatcaa ttgcatcatg tattgctttt 12000 tattcctggg atttatttca aagttaacag gttcctgaaa ggacttttct tcatcttgca 12060 ttgtgtttgc tacctgtacc tcatcccaga aataaaaatt tttgtttact tcgagatctt 12120 tttactttat actaattaca agtgaccaga ttgttctagt ttaaatttgc aatcaccata 12180 tttgagatca aactgtgaca aatcccacca cccaaactta gtgcgtaact gagttttagg 12240 gaatcggtag cccacagtac gtgcttctcg aatatctaaa gcgtagtgtt cttcaccaac 12300 atgaccataa gacttcacat atgccttaca aaatggctcg ggagctggca cacaacccgt 12360 caacactact gctgcaacta cgatggctag taactttttc ataatcgttc ctctgtttaa 12420 tttataaata tattatagca aaatagaggg atctaagcaa atacattttt aaagaaggag 12480 attgtatgag ttttttcgca ggaaaattta gtgatggtaa aactgtatta tctctaaacg 12540 ctgctaatgg tggtgatata aatcagcact actctccaaa tactaatagt atcttccact 12600 cagatatgcc atttgtccta gttgatggga cttacgaggc cgctctaggt gatgcaggca 12660 atgggtattt tgttactcag atgccttggg atattataaa cattaaatcc aatgatcctg 12720 gtagagttat actaactgct attgagataa acggtactca tcgtggcttt ctgaatggta 12780 cacaatctca ggttggccaa ttcttaacat tcttcgaaga tcaaccacgt gctggtgctg 12840 aactggctat aacatctgct tttgcaatcg gtgatagttt agctcacggt acttatatat 12900 acaatggtgg tcttggtcat gaggaatcta ttgctaggca aggaactggt ggtactttac 12960 tacaatcttc cggtttccag atctttagac ctggtggtgt ttccgcaggt gaagctattt 13020 ccagagcctg gagtcttatg ggttttccaa ctggtgtctc tactgttcct ttagatggtg 13080 gtaacgcaga ttattgggaa cctaactggc aggcgcctat tggttctgct ggtagaggcc 13140 atgattggtt ttatgtttgt agttctaata ttcgtggatt tgtaggtaaa aaaggggaga 13200 taccttctaa tgtaaacatt aactaccaat ctcctgcata cccagaaaaa atatatgttt 13260 gtagaggttc tacttctaat atggctgctc agtctgctag aaaggtatat gtacaggact 13320 ggtataacgt tacgccaact aaggttattt ggtatgtgct aaatctgcgc tattctaatg 13380 gtagtatggg agtttctggt aatccattta ccggttcaga tatcttaata tctccgtcta 13440 attttactat taaaggtgtt agcctaccaa atacaggcta taaatttatt aaccagaatg 13500 ctttcggaaa cttaggctat cgccctgata tggagtatgt tggtaataac gctgcgtata 13560 ccggagtttt tggtgataat actgcacgat gtgaatttat cggctctagt aatggcggat 13620 tgtggtctcc tgttaactat gggggtgcag cttctcaaat tagcttatat aaatttggtg 13680 taggtaaaca atggtatgta gatactaata ccaactccat tggtaatgaa catggacctg 13740 tgtggagtcc tagtactgta cccctcaggt tattcccagg caacgttgca agtacttatg 13800 taggggacga tataactcca tcctaccctg gtgctggtga cttctacaca cctttggcca 13860 cagtttggtt gggattacca aatgctcact ctactgttat attaactact gaagttatta 13920 tgggtaatct taatactgct ggaatgcctg tgcgtactta cggcggtagc gcttggcagg 13980 tacaaggtag acgtcaacaa agttatacag ctggtgatgg tgtattccac cagattctta 14040 ctctgccacc tggatatcta gtaccttatc acagtacaac ttcctatagt tatacacaga 14100 cttgggctag taggcctgat gagtttgctt tccgtaggaa cggatatatt tatactatta 14160 aaaaccttgg taatggcaac gttgagttgg gtgttatcat tcacgctgca gaagcggcag 14220 ctgttttctt gccaagacta cgtgtaacag ttcaacgcct aacctaaagg aggatatatg 14280 gcaaatgatg tattagttcc cgatctcatg tctcccgaag gcatggatgt tattgaagcc 14340 tatttacagt gcggtagtga tgttcctgcc gccgctcgta gtcttggaat gtccgagatt 14400 gcattccgtg atataatgaa tcgtggggaa gttaaaaact atctcaacga tatctttatg 14460 gaaagtggct tccgtaatcg tgaccgctta ttcggagttc ttgacgaagt tatcaagcgt 14520 aagctggaag aacttgaaga aactggcatg ggttctgacc aggatatcat ggatattctc 14580 tggaaggcgc acaagatgaa gatggaagaa atgaaaatga tggtagaact ggagaaagtg 14640 aaggcagcag cccgtactcc ggctaatcag actaatattc agaataatat tattgctgga 14700 agtggtgacg aaaactatat gaatcttatt accgctctag caggaggagg taagagataa 14760 tgaaagtctc tagagactat attaacacca cagatgttat tgactttggt attgacaaac 14820 gattcttccg tctgccagtc tctagcattc tagctcaaga ggggattaca cctaatgccc 14880 ctcagattgc aatcattaat gcgctggaag acccaagaca ccgcttcgtg actgcatgtg 14940 tatcacgtcg tgtaggtaag tcattcatcg catatacact tgggttccta aaacttctgg 15000 aacccaatgt gaaggtgctg gtagttgcac ctaactactc actggctaac attggttggt 15060 cacagattcg tggtcttatt aagaagtatg gattacagac tgaacgtgag aacgcaaaag 15120 ataaagagat tgaactagcg aatggttctc tctttaagct ggcttctgcg gcgcaggctg 15180 actccgcggt tggtcgttca tatgacttca tcatttttga cgaagcagcg atttccgacg 15240 ttggtggtgc agctttcgat attcagttac gtcctactct agataagcca aactctaaag 15300 ctctgtttat ttccacacct cgtggtggta actggtttaa agagttctat gagaaaggat 15360 ttaacgaaac gctacctaac tgggtatcta ttcatggtac atatcgtgat aacccacgtg 15420 ctgatctgaa cgatatcgaa gaagcgcgtc gtactgttag taaaaactac ttccgtcaag 15480 aatatgaggc tgacttctct gtattcgaag gtcagatctt tgacaccttc aatgctatcg 15540 aacatgttaa agacctcaaa ggtatgcgtc acttctttaa agaggatgag gctttcgaaa 15600 cattacttgg tatcgacgtt ggttatcgtg accctacagc agttcttact atcaagtatc 15660 actatgatac tgatgtttat tacgtactag aagagtatca gcaggctgaa aagactacag 15720 ctcaacacgc tgcttatatc caacactgca tagatcgtta tagtgttgat cgtatatttg 15780 tagactccgc tgctgctcag ttccgtcagg acttagcata tgaacatgaa atcgcttctg 15840 ctcctgctaa gaaatctgtc ctagatggtc tggcatgcct acaagcactg ttccagcaag 15900 gtaagatcat cgtagatgct tcatgtacct cattgatcca cgccctagcg aactacaagt 15960 gggacttcca ggaaggcgaa gagaaattat cacgtgaaaa accacgtcat gatgcgaact 16020 ctcacctttg tgacgcactg cgttatggaa tttactcaat ttcccgtggg aaataaataa 16080 gtataggatg ggatactact cagttggtat cccattcctg cattttaaaa tcccctttac 16140 aaattcgaca tgattatgta tactatattc atacagttga ggagaatact gtggcaggaa 16200 atgtaaaata taaacgtgat gctatatcac tcatgcgaga cggtataaaa agtcaatata 16260 aaaaagccga tcgctgcgaa atctgtggtt gtggtgaaga attagagctt catcattacc 16320 atacagtgtc tctactagtt aaaaaatttg ctaaagaact ccaattggat ttcactgacg 16380 aagaaacagt cctctcaaat aggacggcat tctatgaaag atataggcat gagctagtag 16440 atgacactgt taccctttgt gtccaccatc accaactatt acataaggtg tacactaaag 16500 aacctcccct tttctctgct aataaacaga aggcatgggt tcttaaacaa aaggaaaagc 16560 tacagaatcc tcaagaaaag acacaagtca agactgaaac taaatcagga ttcgcaaggt 16620 tcttataatg ggtttaaaaa gctggattac tgaaaagcta aatccgggtc aacgtattat 16680 aagagacatg gaacccgtta gtcatcgcac taaccgtaag ccatttacca ctggacaagc 16740 ctacagtaag attgagattc tcaaccgaac tgctaacatg gttatcgata gtgcggcgga 16800 gtgttcttat actgtcggag ataaatataa tattgtcacg tacgctaatg gcgtcaaggc 16860 aaagactcta gacactctct taaatgtacg acctaatcca ttcatggata taagcacatt 16920 ccgtagactt gtagtcactg acctactttt tgagggttgt gcatatatct attgggatgg 16980 cacagcactt tatcatgtcc ctgctgcact aatgcaagta gaggcagatg ccaataagtt 17040 tatcaaaaaa ttcatattta ataaccagat agactatcgc gtagatgaga ttatctttat 17100 aaaggataac agttacgtgt gtggcacaaa ctcccaaatt tctggacaat ctcgtgttgc 17160 tacagttatt gactctcttg agaagcgttc taaaatgctt aactttaaag agaagttcct 17220 cgataacggc acagtgattg gtcttattct tgagacggat gaaatcctga acaagaaatt 17280 gcgtgagcgt aaacaagaag aattacaact cgattataat cctagtactg gtcagtctac 17340 tgtcctgatt ctagatggtg gtatgaaagc aaaaccgtac tcccaaatat cctcttttaa 17400 agatctagac ttcaaggaag atatcgaagg atttaataaa tccatctgtc ttgcctttgg 17460 tgttccgcaa gtgttacttg atggtggtaa taatgctaat attcgaccaa acatcgaatt 17520 gttttattac atgactatca ttcctatgct gaacaagctg actagttctc ttactttctt 17580 ctttggctac aaggttactc ctaatactaa ggaagtagct gcactgacgc cagataaaga 17640 agctgaggct aaacatttaa cctctttggt taataacggt atcatgactg gtaacgaggc 17700 tcgtgaagag cttaaccttg aacctttaga tgatgagcag atgaataaga ttcgtattcc 17760 tgctaacgtt gctggttctg caacaggtgt atctggtcaa gaaggtggta gaccgcaagg 17820 cactgccgat ggggataaag aatgattgat tatgatggtc taaaggccat ttttggtgaa 17880 aagctgccag aatctcatat cttctttgct acggttgcag cacacaagtt cgttccaaac 17940 tacgctactc tacgtaaaga gtttggaatg accactgcac ataccaaccg caaggtttgg 18000 aataagttata agaagcata cgaagttact gctccggttg ttccggctct agctttcacg 18060 aagaatctgg ctaaaactct agctgttgat actggtgcag caattaatct gggcgtaaca 18120 gttactggtg gtactgcacc atatacttat gcttggacta aagacggttc tccacttagt 18180 ggtgttactg gccctaattt caacaaagct acagctgctg cagaagatgc tggtacttac 18240 aaagttgttg taacggatag taaatctact tccattactt ctaatgagtg tgtaacaact 18300 atcaaccctg cgccggaggc ttaataaatg acaaatgctg ctattgacta taacaagtta 18360 aaatcagcac cggttcattt agacgcttac attaagtcta ttgatagcga atccaaagag 18420 ggtgttgtaa aaatccgtgg attcgctaat acaattagta aagatcgcgc tggtgacgta 18480 attcctgctt cggcatggaa aacgtctaat gcacttgcta actacatgaa aaacccgatt 18540 attcttttcg gccacgacca tcgtcgtcca atcggcaagt gtattgatct taaccctact 18600 gaaatgggtc tcgaaattga atgcgagatt tatgaaagtt ctgatccagc tatcttctca 18660 ctaattaaaa acggtgtact gaaaaccttc agtatcggat tccgctgcct agacgcagaa 18720 tgggatgaag ctactgacat atttattatt aaagatttag aactatacga agtttcggta 18780 gtttctgtac cttgtaatca ggactcaacg ttcaacctcg ctaagagtat gaatggccac 18840 gattacactg aatggcgcaa atcttttact gcaacaagtt ctaaggctgt cccagctcaa 18900 gaacgtaatc tttctgaact agaaaaactt gcgatagctt taggctacgt taaagaataa 18960 cggagaatta tttaaaaatg actattgata ttaataagct gaaggaagaa cttggtctgg 19020 gtgatctggc taaatctctg gaaggtctga ctgctgctca gaaagctgcg gaagctaagc 19080 gtctgcgtga agagcaggaa gaaaaagaac tggctcgtat gaacgaccta gtatctaaag 19140 ctgttggtga agaccgtaag cgtctggaag aagctctgga tctggttaag aacctggatg 19200 agaagtctaa gaagagcgca gaactgtttg cacagactgt agaaaagcaa caggaaacca 19260 tcgttggtct tcaggacgaa atcaagtctc tgttggcagc tcgtgaaggt cgttccttcg 19320 ttggtgatag cgtagctaaa gctctttatg gcactcagga tgctttcgaa gatgaagttg 19380 agaaactggt tcttctgtct tatatgatgg agaaagacgt attcgaaact gaacacggta 19440 aggctcacgt taaagctgtt aacggttctt cttccgtttc catgtctagc gaagcatacg 19500 aaactatctt ctctacccgt attatccgtg acctgcagaa ggaactggta gttggtgcac 19560 tgttcgatga actgccaatg tccagcaaaa tccttactat gctcgttgaa ccagaagctg 19620 gtcgtgctac ttgggtagat gcttccaaat tcggtactga cgaaactgtt ggtgacgaag 19680 ttaaaggtca actgactgaa atcagcttca aaacttacaa gctggctgct aaatccttca 19740 tcactgatga aactgaagaa gatgcaatct tctccctgct gcctctgctg cgtaagcgtc 19800 tgatcgaagc acacgctgtt tctatcgaag aagctttcat gtccggtaat ggtactggtc 19860 agcctaaggg tctgctgaaa ctggctgctg atgatggtgc taaggttgtt accgaagcta 19920 aagctgacgg ttctgttctg gtaactgcta agactatctc caagctgcgt cgtaaactgg 19980 gccgtcacgg tctgaaactg agcaaactgg tactgatcgt atctatggat gcttactacg 20040 atctgctgga agatgaagaa tggcaggatg ttgcccaggt tggtaacgat gctgttaaac 20100 tgcagggtca ggttggtcgt atttacggtc tgccggttgt agtttctgag tacttcccag 20160 ctaaagctgc ttccgctgaa ttcgcagtta ttgtttataa agataacttc gtaatgccgc 20220 gtcagcgtgc cgttactgtt gaacgtgagc gccaagctgg caaacagcgt gatgcatact 20280 acgttaccca gcgtgttaac ctgcaacgtt acttcgaaaa cggcgttgta tctggtgctt 20340 acgctgcatc ttaatacagg cttttcagcc gataaggaga gcttcggctc tcctttttta 20400 ttgggaaaaa taaatgcaaa tcatcactgt tgaagattat aaactatatg ggaatttaaa 20460 acgtcccgat atggaaactc aggttgagat gatgattaaa gctgccaata cgctgattac 20520 aagccttcta ggtatggatg atgcagatgc ggtagatcaa ttaattacca caaaacctag 20580 ccgtaggaaa tatttcctga gttctccttc agccacatct attacaaaga tgactattaa 20640 cgagaatgaa atagatccgg aacagtttaa actgtatcct gatggtgtta ttctccttaa 20700 gtttaatcct cctgaaggat atatggatgt agagtatact caaggtggtt tcaatccaat 20760 gcctgaagat cttaaactgg cagcatgtct gttagtagat cactggcata agcaagatta 20820 tcgtcaagcc aagactattg gtggtgagac agttacgttt aacaatacta agtctggtat 20880 tccagaacat attcgtacaa tcatcgaagt ctataggaga gtgtaatggc tctttctgat 20940 ctagctagac aaattattaa agagcagcta gatactgcag gacgatctga gaataataag 21000 aatactgttg tgtatacagt tgagacaggt ttgaaagatc ctacaagaga tggtacagtt 21060 gcacaggtat ctttcaaatt ctcgaaacca gtatcacaag acttattaaa tgttaggaca 21120 gcatctatac tgaaggctgt ttcttctaac ttagatcttt ctggtgattt aggtgctcta 21180 gagaatctaa ttcaagcaac cgcaggtaaa cgttccgccg ttggtaaaaa acgttctacc 21240 ggtagagttc aggtaaactt tggagatcct agagatactg aagatggcta ttctggtgca 21300 gtaacaggtg cttccggtcg tttcgtatcg aatagtaaca tgcgtgctat tcttgagata 21360 gtagctaagg aatacttaat caaggatatg aaaaaagcag gggcaccatt gaagttcaga 21420 actggtcgct ttgctaactc cttaaagatt aaagacgtta tgctacgtga tgcagaaact 21480 agtaaaggtg ctccagagct gaatgtgacg tataactata tggttcgtcc ttactctgtg 21540 ttcaaccccg cagtttctac atatcgaaga ctttctctac gaccttaccc tggcgctcga 21600 aaccctcaaa aacttattgg ggaagcaatc gcaaaagctg caagagacct gattcactct 21660 agatataaaa tcaaggttaa tcaaggaacc tagtaaatgg atcacagaac aagtattgca 21720 caagcagtgg ttgaccgaat cgccacacaa atggatggtt cacaacctga cgagtatttc 21780 aataaccttt atggaaacgt ttctcgtcaa acttataaat tcgaggaaat ccgagaattt 21840 ccttatgtag cagtccatat cggaactgaa actgggcagt atcttccctc aggccaacag 21900 tggatgtttt tagaacttcc aatcctcgtg tatgacaaag agaaaacaga tattcaagag 21960 caacttgaaa aactcgtagc tgacataaaa accgtcattg acacaggtgg aaatttagaa 22020 tatactgtta gtaaacctaa tggatcgacc ttcccatgtg aggcaactga tatgagcatc 22080 acctcagtag ctacagatga gggtctactg gccccatatg gtttagcgga aataaatgta 22140 acagtgaggt atcagccacc tcgtagggca cttcgcagat aagttacaga ttaggagaat 22200 ataaataaat gtctttacaa ctattacgta atactcgaat cttcgtgtct acggttaaga 22260 ctggtcacga taagaccaac acgcaggaga ttctagttca agatgatatt tcttggggtc 22320 aggacagtaa ctcaaccgat attactgtta acgaagctgg tccgcgtcca actcgtggtt 22380 caaaacgttt caacgactcc ctgaacgcag ctgagtggag tttctctact tacatcctgc 22440 cttacttaga taagactact agtaagcaga tcgttccgga ctacatgctg tggcatgctc 22500 tttctagtgg taagcctatc aacctagacg gtgatacagg tgctcacact aacgaaacta 22560 acttcatggt taactttaag gataacgcat accacgagct ggcaatgcta catatttaca 22620 tccttactga taaagcatgg agctacatcg attcctgtca gatcaaccag gctgaagtaa 22680 acgttgatat cgaagatatc ggtcgtgtaa cttggtcagg taatggtaac cagctgatcc 22740 ctctggacgc tgctcccttt gatcctgata ctgtgggtat tgatgatgaa acttacatga 22800 ctatccaggg ttcttacatc aagaacaaac tgactatcct caaaattaag gatatggata 22860 ctggtaaggc atatgacatt ccgatcactg gtggtagctt tacaattaac aacaacatca 22920 cctatctgac tcctaacgtc atgtcccgtg ttaacatccc aatcggttca tttactggtg 22980 cattcgaatt aactggttct ctgaccgctt atcttaacga taaagctcta ggttctatgg 23040 atctgtataa agatctgatt aagaccctga aggtagttaa ccgtttcgaa atcgctctag 23100 tacttggcgg tgaatatgat gatgagcgcc cagccgctgt tctagttgct aagcaagctc 23160 acgttaacat cccaactatt gagactgacg atgtacttgg tacttctgta gagttcaaag 23220 caattcctac tgatctggat actggtgatg aaggttatct tggtttctct aataagtaca 23280 ccaaaactac tattgcaaat ctgattgcaa ctggtgacgg tggtaaagca gctccggtta 23340 aggtaacagg tattactgtt aaatccgcgg gtgatgcagc ttccgttgat aatagtgcaa 23400 ctcttcagat gactgtagat gttttgcctg ctgaagctac taataaggaa gtaacttggt 23460 ctatttcctc cggtactgct gctactattg atgcaacttc tggtttactg accgctgatg 23520 cttctaagac tggtgcagtt actgtgaaag ccgtagctaa agatggttct ggtgtagaag 23580 gtactaagag cattacagtt actgcttctg gtggctaatt aaatgtacta ctctctaatg 23640 agggagtcaa aagttatagt tgagtatgat ggcagggctt atcattttga tgccctgtca 23700 aattacgata tcaatacgtc ctatgaggaa tttaaaactc ttcgtaggac tatccatcgt 23760 agaaccaact atgctgactc tattattaat gcacaaactc catcttcaat atcgctggct 23820 ataaatttta gtaataccct tactgaagct aacttctttg aatggatggg ctttgaccga 23880 gagggtaaca cattcttact accactttat agtacaaata ttgagccaac aatgtttaat 23940 atatacatag ttaataaaga taataactgt gtgtactttg agaattgcta tgtatctact 24000 gtagactttt ctctagacaa aagcgttcct atcctaaacg taggaattga atctggtaag 24060 ttctctgagg tgtctacttt tactcctgct ggttctattg ttcagggtga agttatgtca 24120 tatagtcctc ctagagtatc taccaacagt aatgttttac cagctctttt atccgcttct 24180 atgtcattcc agcaacaatg ttcttggaga gaagataaaa gcgtatttga cataaacaaa 24240 atatataata ataagagagc ttatgttaat gaaatgaatg cttcggcaac aattgcattt 24300 tactacacta agcgatttgc tggtgatatg ttttataata ttgaacccga aactgacata 24360 ccattaacca tccgaaatga acatatttcg attgactttc cttcagcacg tataacaaaa 24420 cgcctacaat tctcggatat atacagagtt gaatgggatg taatccctac tgcagactct 24480 gatccggtaa gaatcgactt ctttggagaa ataaagaaat gatcaattta aaagatatta 24540 cccttgaaac tcgtactatt gagctggctt acccaggtat gcctaacttc aagctgcaac 24600 tgaactacat gtctcgtgct gcatctaagc gtgttatctc ttccgctaag cgtgatgagt 24660 gggttaacgg tactatgatt caggttcagg acgatgataa gtttattgaa gctttcgttg 24720 atactgcaat tgctggctgg actggcttga cgattggaga tgttgagaag ctgatgctta 24780 tcgaaactga agctgatcct actactgaag ttccgtttag tcgcgataac gctatcatgc 24840 ttatgcagaa ctctgctgcg tttgattctt ggatcaacca gacagtgttc cacttagaca 24900 cttttcgtag ctcaaaagcg taagcagtta ctagatgctg ttgccgattt tgcagataaa 24960 tgcattaaat cctcagcatc taaaatgaca aaacaacaat atttaatgct ttgcgagtct 25020 ctaggtattg aacctgatcc taaggcaatg cctgttgaac tcgaagattt tccacctatt 25080 gtatctacta gtatgaatat ttataacagt cttattgact gtttcatacc aggtgacttt 25140 cctatcttta taggaaaaga caaaagtgca ttaggtgttt tatttgatat ctatgggatt 25200 actgatccta tagagaaaga gtttgttcta cacatcatca atatatttga ttcgaaagct 25260 gtagatgcag ctcgtaagag agccgagaag tatagtaaac ctaatggtcg taccccgaac 25320 attaaaccac atgctagatc tcgagctcag taaaagtttc ctccaatggg cgttccacga 25380 tgaggcttgg ctctgggtga tttgcccaga gcctttttta ttgggaaaaa taaatgactg 25440 ataagctaat acgagaacta cttatagatg taaagcagaa aggggcaacc cgtactgcaa 25500 aatctattga gaacgtttcc gatgcgttag aaaatgctgc tgccgcttcc gaactgacta 25560 atgagcagtt aggaaaaatg cccaaaactc tttactccat tgagagggcg gcggatagag 25620 cagctaaaag tctaactaaa atgcaggcta gtagagcat gactggtatc accaaatcta 25680 tggatagtat tggtgctaag ttagatgacc ttgctattgc tatgatcgag gtatccgata 25740 agctagagtc tggatttaca cacgttggta aatctgttaa ggcgatgggt aacgatgtag 25800 ctgctgcaac tgaaaaagtt caagatagat tatatgatac taaccgagca ctaggtaata 25860 ctgctagagg ctttaatgat acaactaccg ctgcaggacg tgctagtcgt gcaattggta 25920 atacttctgg ttcagcacgt ggtgcaactc gtgactttgc agctatggct aaagtgggtg 25980 gtagtttacc tcttatgtat gctgctatcg cgtctaacgt gttcgttctg caatctgcat 26040 tcgaacaact taagatgggt gaccagttaa accgtcttga gaagtttggt actattgttg 26100 gtactcaaac aggtactcct gtacaaactc tagctagatc tctacaagag gctgctggtt 26160 atgcaatctc cttcgaagaa gcaatgagac aggcatcctc tgcttctgca tatggattcg 26220 acgctgaaca acttaataaa tttggtctag tagctcgtcg tgctgctgct gttcttggtg 26280 ttgatatgac tgatgcactt aaccgtgtaa tcaagggtgt atctaaacaa gaaatcgaac 26340 ttctagatga acttggtgtt actattcgtc ttaacgatgc ttacgcagac tacgttaaac 26400 agttaaatgc tgctaacaca ggtattacat ataacgttaa tagtctctct actttccaga 26460 agcaacaagc atatgctaac gcagttatcg ctgaatctac taaacgtttc ggctatcttg 26520 atgatgtact tcgtgcaact ccatgggaac agtttgctgc taatgctgat gctgcactga 26580 gaaaggttca acaggctgct gctaagtatt tagggccagt aattgattct atcaacgcag 26640 tgttctatac ttctcaagca tctatctcgg cctctgctgc tagagcacag gaagaaacta 26700 accgtcagat tgacccaact aacgttggtg ctgtagcttt aagtctttcc gcttctgagg 26760 aaggatataa taaagctctt gatatgtaca aagaatctct agaaaagcgt aataagttaa 26820 aagctgatct agataagcgt atgcagcaag cagatgcatc tacagcaggg gctattcgtt 26880 tagttgctca aggtgcaccg gtagggttag ctgcaggagg ttttgaagta ggaggagttc 26940 gcataggatc ttctgaagaa aaccagaaat ttattgcaga tactgctgca ttagctctcc 27000 aggtagagcg tcttgataag gaagtagagg attcaactgg caatcttaat gcatggaaat 27060 cggcatacca agctgctggt gctgcagctg ctaaggctaa tccagaattc cagaagcaaa 27120 ttaacctaca gaaagatgca aatgaccctg atgcggttta tgacttcaac tctgcaactc 27180 tgaaaggact gacagaacag cagaaagcat atgatcaggc taagaaaact gctagtgatt 27240 tagctaacga tatccagaac atcgctcaga atactaatac tgcggctaaa actagtgcat 27300 ccttatcaga tacgattaag actattgagt ctctatccgc gggtactggt aaaaatgctg 27360 atgagtatgt taagagcctg aacttagggt ataatactct tagtgagatg aagactgctt 27420 cacaagctct tgctggttat gtaaaattaa ctggtaatga gactaagaat cagctagaag 27480 ttcagcagaa gattgccgaa gtgtatagcc agactaagga caaagagaaa gcacaggaag 27540 ctggtagacg tctggaaatc caacagttag aagaacagga agcagctctt aagcgtgtcc 27600 tagaaactaa caagggtaac aaagcaatcg aaggtgagat agctaagatt cagctagaac 27660 gtatcaagat cactaaccaa ggtatggaag ctcagaagaa ggttaaggac tataccgata 27720 agattcttgg tgttgatcgt gaaattgctc tcctgaataa ccgtactatg acagatactc 27780 aatatcgttt agctcagttg aaccttgaac tgactatcga gaaagaaaag tatgaatggt 27840 actccaaaca ggttgataag cataaggaag ctgaacaatc aagacgtgct caggcacaaa 27900 ttgaacgtga aatctggaaa ttccgtcagg atcagcaaag gggaatggct gctggcaggg 27960 aagaggagca acaaaggaaa tttaccgcaa atcctttaat gggagctgcg gaacgtatca 28020 aggagcaaat ccagctattc gaagacctta agcaaaaaac tttagggaat gctgctgctc 28080 agttagagta taataaaaag ctagcagaaa ctagagccca gctcgcagct ttaagagtac 28140 agcgcgatgc agaaatgcag tcttctgtag gtgcggctgt aggggctacg tatactccta 28200 ctactggatt aactggagaa gataaaaact tcgcagatat gcagaataga atggcgtcct 28260 atgaccaggc tatttctaag ctgtctgaac tgaactccga agcaaccgct gtagcacaaa 28320 gcatgggtaa cctaactaac gctatgatcc agttctcaca aggatctctg gataccactt 28380 ctctagtagc ggctggtatg caaactgtgt cttcaatgat tcagtatagt gttggtcaac 28440 aggtaagtgc tattgatgca gctattgcag cagaacagaa acgtgatggt aaatctgagc 28500 aatccaaagc taagatcaag aagttagagg ctgaaaagct caagatccag caagatgcag 28560 ctaagaagca aatcattatc cagacggcag tagcggtgat gcaagcagca acagctgttc 28620 cgtatccatt ctctattcct ctgatggttg cggctggttt agcaggtgct atggctcttg 28680 ctcaagcatc ctctgcctct ggtatgtctt ctattggaga ctctggtgct gagacagcgg 28740 gttacttaac tcttggagag cgccagaaga atgtagacgt ttctatgtcg gctaacgcag 28800 gtgaactttc ttatattcga ggagatcaag ggattggtaa tgctaactct ttcgtacctc 28860 gtgcagaagg tggtaatatg tatcctggag ttagttacca gatgggtgag catggtaccg 28920 aagtaattac tcctatggtt ccgatgaagg ctacccctac tgatgaactc aaaggatctt 28980 caaaaggcac atcgggtaga cctattgtgc tgaacatcag tacgatggat gcggctagct 29040 tccgagattt tgcttcgagc aatagtgctg ctttcaggga tgccgtggaa caggctctta 29100 atgagaatgg aaccaccctg aaatcacttg gtaattctta atactggagg aggactttat 29160 gtcctccttt tctttatgga aaaataaaaa tttcttgata aaattttcca atcctattat 29220 aatattgtta ttaagaggag aaattaacta tgagactacc agacccatac acgaacccag 29280 agctatcagg gttaggtttt gaaagcgtta acttagtaga caatgaccct atgattcgtg 29340 atgaattacc taatggtaaa gttagggagg ttaaggtgtc tgctcaatat tggggtatta 29400 atatctctta tccagaatta ttcccagatg aatttgctaa attagattca ttcatcctag 29460 attataaaag aacaggtagc tatattgatg tattattacc acaatatgaa gctttccgag 29520 ttcgtggtga tactagtgct gtaaatattc ctgctggaca aaaaggttca acagttatta 29580 tggacactaa gggtacactt cgtggactgc ctaaaccagg cgatctattt aaactgtcca 29640 accacccaaa agtgtacaaa atcacttcta tcaacacagc aggaaatact tggaatataa 29700 gtgtttaccc tgatctattc attactacta cgggtagtga gaaaccggta tttaatggta 29760 ttctatttag aaccaaatta atgaacggag acagcttcgg ctctacgtta aatagcaatg 29820 gtacatatac tggtatttca ctttccttga gggaaagtct atgaagaaaa ttcttgacag 29880 tgcaagagag tatttaagaa ctcataataa aataaaaact gcatgtctta ttactcttga 29940 attgcctggc tctactggat caagttctgc ctacatatat cttacagatt attttagaga 30000 cgtaatttat aatggtattc tttatacatc tggtaaagtt aagtctataa caacgcataa 30060 acaaaataga aaactttcca ttggtagcct ttcttttact atcacaggta ctgctgagga 30120 tgaagtatta aaactagtcc aaaatggtgt atccttccta gatagatcta tttcaattca 30180 tcaagcggtt attgatgaag agggtaacat tcttcctgta gatccagaca ctaatggtcc 30240 tttactttac ttccgtggta aaattactgg tggtggtatt aaggataaca ttggtacttc 30300 tggagttagt acatccgtta ttacatggaa ctgttcaaac caattctatg actttgatag 30360 agtaaatgga cgtttcacag atgatgctga ccatagaggg cttgagattg ttgccggcca 30420 attagttccg tctaatggtg ctaagcgacc tgaataccaa gaagactacg ggttcttcca 30480 cgctaacaag agtatctcta ttcttgctaa gtatcaggtt caggaagaac gttataagtt 30540 acaatctaag aaaaaattat ttggtctttc tagaagctat agtctaaaga aatattatga 30600 gactgtaact aaggaagtag atcttgattt taacttagct gctaaattcc tgccagtagt 30660 atatggagtt cagaaaattc ctggaatccc agtatttgca gatactgaac tgcataatcc 30720 taatatcgta tatgtagtat acgctttctg tgagggtgag attgatgggt tcctagattt 30780 ttactttggt gatgtaccaa tgatctgtat tgatcctaat gatagtaagt ctcgtacttg 30840 ctttggtact aagaaagtag ctggtgatac catgcaacgt attgcttctg gtcaaccaag 30900 ctctgaaccc tctgttcatg gacaggaata taagtataat gatggtaatg gcgatatccg 30960 tatctggact tatcatggta aggccgatca atctgctgct gatgttctag ttaacattgc 31020 aaaagagaaa ggtttctatt tacagaacat gaacgagaat gggccggaat actgggactc 31080 tcgttataaa ctgctagata ctgcttatgc tatcattcgc tttactatta atgaaaacag 31140 aacagaaatt ccagaagtta gcgctgaagt tcaaggtaag aagattaaga tctatcattc 31200 agatggtaga gtaactgctg ataagactag cttaaatggt atctggcaaa ctctggacta 31260 cctaacctct gatcgctatg gtgctaatat cactattgat cagttccctc tccagcaact 31320 tattcaagaa gcagctattc tggatataat tgatgaatcc tatcaggttt cttggcaacc 31380 ctactggaga tacgttggat ggactgatgc tgtaggagag aatagacaaa tagttcaaat 31440 gaacactatt ctcgatacct ctgagtcagt atttaaaaac gtgcagggat taatagagtc 31500 ttatgggggt gctatcaaca acttatctgg tcagtataga ataactgttg agaagttctc 31560 gaatacccca ttagagattg acttcttaga tacctatggt gatctggaat tgtcagatac 31620 tactggtaga aataaattca actcagtaca agcctctata ctagatcctg cattaagctg 31680 gaaaactaac tccattacat tctttaactc tatatttaag gaacaagata aagggttaga 31740 taaaaaactt caactttctt ttgcaaatat cactaactac tatactgcgc gtagctttgc 31800 agatagagaa ctgaaaaagt ctcgttactc acgaactctc acattctcat taccatatca 31860 tttcataggc attgagccta acgatccgat tgcttttaca tacggtcgtt atggttggga 31920 caagaaatac ttcctagttg atgaagttga aaactctaga gaaggtaaga ttaatattac 31980 actgcaagag tatggtgagg atgtgttcat taactccgag caggttgata atagtggtaa 32040 cgatatccct gatgttagta ataacgtact gcctcccaga gactttatgt atacaccaac 32100 accaggtgga caagtagggt ctattggtaa gaatggtgag ctatcttggc ttcctagctt 32160 aactaataac gttgtttatt actccatcgt tcattctgga catgctgatc cttatatcgt 32220 acagcaacta gaaactaatc caaatctacg aatgatccaa gaaattattg gagaacctgc 32280 tggtttagca gtctttgaga ttagagctgt agatattaat ggtagacgaa gttctcctgt 32340 aacattatca gtagaactta actctgctaa aaacttaagc gtcgttagta acttcagggt 32400 tactaataca gcttcaggag atgcttcgga gtttgtagga ccagacgtta aattggcctg 32460 ggatagaatt ccagaagaag atatcattga tggaatcttc tacactctcg aaatctacga 32520 taacctagat cgtttattaa gaagtgtacg aattgaagat cagtatgtct acgattatct 32580 actgatatac aataaggcag actatgctct tcataatgag gatgctctgg gtattaatag 32640 gaagttacgc ttccgtataa gagcagaagg tgataatggc gagcaatctg tggattgggc 32700 atctatttaa atgatttcga ataatgcacc agccaagatg gtcttaaata gtattatgac 32760 tgggtatacg atggcgtata tccagcattc tatctatacc gactacgatg ttatcggtag 32820 atcattctgg cttaaacttg gcgaagaagt ggatagaaga gatttcactg gaatcgacac 32880 tttctttgtt atgatcaata atttaactcc ctcaactacc tatcaggttc agggagcttt 32940 ttatgactcg attattgact cagaactttt aaatgcaaaa attggtatta acctctctaa 33000 tgaaactaac tttaaaacaa aagagaagcc aataattgtc gcagcaagat ctgagtcaga 33060 acctgtggat gttggggtgg gcgcaccgat agttgttgtg gaaacaactg gtgaagcaag 33120 ttactgtact attgagttaa aaagtacagc tactgaagac agtccttgga ctaaatatta 33180 tattggggct ttaggttcta ctattaaatt tggtggagtt cctatcggag actataagat 33240 cagaatatct ggtcaagtaa ctatgcctga tggtgttaca gttgattctt caggatacta 33300 cgagtttcct aatattctaa ctgtagctta taactttgtt ccacctactg cacctatcga 33360 tattgttttt aaagctgcac gaattgctga tggtaaagaa cgatatgatg ttagaattga 33420 gtgggattgg gaacgtggtg ctggtgctaa cgttcgtgaa tttttagtta cttatataaa 33480 ctccgaagaa tatgctaaaa ccggatgggc taaagctcaa aagataaacg tgggtgctgc 33540 tagagctgca acgattatat cattcccatg gaaagttgaa cataccttta aggtttcatc 33600 aattgcctgg ggaccaaata agcaagatat aacagaatca gctcctgtaa catttatatt 33660 gaatgaagat actcctctag acaacagctt tgtcaatgag actggtattg atgttaacta 33720 cgcctttatt aagggcagca tgaaagatgg ggaaatctgg agacagacat tcttaattga 33780 tgcagctact ggtgctatta acattggtct gctcgatgaa gaaggaaaaa cacctatttc 33840 tttcgaccct ataaaccgtg ttgttaacgt tgatggtaaa gtaattacta gagatattaa 33900 tgctgcgaac tttatcatga ctaacttgtc tggtaaagat aacccagcaa tttatactca 33960 gggtaaatcc tggggggata ataactctgg tatttggatg ggtatggata atacctctgc 34020 caaagctaaa ctagacattg gtaatgctac acaatggata cgttatgatg gtactactct 34080 gcgcatctct agtggtgtag taattggaac gccaaatggt gacgtagata ttggaactgg 34140 tttacagggt aagcaaacag tatttgttta taagttagca acatctttac cggccaaacc 34200 gctagaacaa gattatccac ctcctggttg gtcaaaaact ccacctaacc gtacagatat 34260 gacacaaaat atctatgcga ctacaggtac acttgatcca gttactaaca aacttcttga 34320 aggtactagc tggtctgatg tagttcagtg gagtggtact gaaggtacta taggacatga 34380 tggacagcgt ggtcctggga tgtactccat gggtattcct ggattaggcg gttgggatga 34440 tggacaagct aacgcattct tccaaaataa ctttggaaaa cccccggtta agtatgatgt 34500 tctaacacaa tttaacagta atgctccaca aacagcattt acccgtcaat ggagcggggc 34560 tggatggatt aaccctgcaa tggttcttca tggcaatatg attgttaatg gaactgttac 34620 tgcggataag attgtggcag gaaatgcctt cttatcacaa atcggtgtta atataatcta 34680 cgatagaaat gctgcgttat cagggaaccc tgaagcatac tacaagatga agatagacct 34740 aaacagtggg tatatccata taaggtaata taatgagtac agaaaaccga gttattgata 34800 ttgttgttga tgaaaaagta ccttatggtc ttatcatgca atttatggat gttgatgata 34860 gtgtatatcc accaacagaa gttcctgtta acttgacggg gtattcactc cgaggaacta 34920 ttaaatctag tttagatgag aacgccgagg ttctagctaa ctttaaaact cgtgttattg 34980 atgctgctca aggtgctgct gcaattagtc tttccgttgc agacgttact aatattggtg 35040 aaaaagcatc taaagaacgt gataagtata acccaagaca acgttttgct ggttattatg 35100 atatcctaat gactcgtgat gtaattggct ctgaagtcag ttctttccgt attatggaag 35160 gtaaagtata catcagcgat ggagtaactc aataatggca cttaaaacta aaattattgt 35220 acagcagatt ctgaacatag aagacactac aactactgct agtaagtatc ctaaatatac 35280 agtagtttta ggtaattcta ttagttctat tactgctggt gaattaactg ctgctgtaga 35340 agcctctgct gcttctgctg cggcagcaaa agattccgag gtagctgcaa agttatcgga 35400 aataaacgct agagattcag aaaacaaagc tgctatatat gctgactctt cagaagcttc 35460 tgcaactcaa tctgctgctt ctgctactga agcggagaga caagctggtt tatctaaaga 35520 tagtgctgat gcctctgcta cgtcagctga ggaatccaaa ggattccgtg attctgctga 35580 acttgctgca caaaatgctg aacagagtcg tctattagct gaacaagcta agaaggacgc 35640 tgaggctgct aagactgctg ctgctacttc tgagcaaaat gctgctacat ctgctactga 35700 gtctaccaat caggctattg ctgctgctgg ttctgctaca gaggcgggag aatacgctac 35760 tactgcaaaa gactccgaga tagctgctaa gacttcagaa cttaatgcta agaattctga 35820 gaatgaatct gctatttctg ctgaagcttc tgaagcttct gcttctcagt ctgctatttc 35880 tgcttctcaa tctgctgcat ccgctactaa agctgcagaa tcatcagatg cagcaaaaat 35940 tagtgaaact actgctatag aatcatcagc tgcagcaaaa actagtgaga ctaatgcaaa 36000 aactagtgag actaatgcaa aaactagtga gactaatgca gcagcatatg cagcagcagc 36060 aaaaactagt gagactaatg ctgctgattc cgctgcctct gcttctgact ccaaaggatt 36120 cagggatgaa gcagaagcat tcgctgcaca agcctccaca tcagcattag cagcaaaaaa 36180 ctcagaaact aatacaaaga ctagtgaaat taactcaaaa gctagtgaag acgctgctaa 36240 gctagctcag caaagtgcat caggtagtgc gaatacagct acgcaagcga tgaccacaac 36300 caaaggctac agagacgaag cagaggtatt taaaaatacc gccactactg ctgcaacgac 36360 agcaacagac aaggccctgg aggccgctgg tagcgctaca atagcaggag agaaagctac 36420 taatgccacc agtgcagcag atagagcgga gacagcggct gcatcagcag aacaggttat 36480 gcaagcgtcg ttaaagaagg atcagaacct taatgatctt gcaaacaaag accttgcaag 36540 ggaggcgcta aaagttgagg ctgttaattc tgtaaaagat caatatgctg gcgcttataa 36600 ttcttttcgt aatcctgcat ggacttatga attacggatc gctaacaatg gggaatggcg 36660 cgttgcgcgt aatgacaata acagcacatc tgcgctttct attggtgctg gcggtactgg 36720 tgctgaaaac gttgagggcg caaaaataaa ctttggcatt gatcgtttaa agcaaacaga 36780 aacagaaact atgatgtatg caccagggag taactcacct tatcgaatca caattagacc 36840 tgatgcggcg tggggtgttt ggactgatga aactggaaga tggattcctc tttcaattga 36900 tgctggcggc actggatcta atactgaagt tggagcaaga aagaacttaa atactcctgt 36960 tggtggtcaa gcaattatta ttcctaataa ttcaaacatt cttgggttta tgtcaacata 37020 cgcagaaagc ggttattact caagcggaga acttgttaca tatcaaccac ctgaagcctc 37080 tggttggtgg atgtatgaat tgcatgttca cggtaaaaat gcaaacggtc atgttgagta 37140 tggaaatatt gttgcaaccg caatgaacgg taataaatgg ggtatcattt gcagtgctgg 37200 ctcttggggt ggatggtaca ggatagcaag atctgatagg caattgatgt tattaagtcc 37260 ggatgctcaa tcggctcttg gtgattactc tatagcaatt ggggatcatg attcaggtct 37320 taaatgggat cgtgatggtc acataagcgc gtttgctgat tcagcgagaa tatttgcatg 37380 gactccatca ggaataaaca cttacagggt catatcatct tatgttgatg ataacgcaag 37440 gggtatgtat gtaaatggag ttaggcgcgg tgatccaaac gctcttattg ctggtcaagt 37500 tgagggtggt tcatttgcca actggcgtag tcgtgcgtct ggattgcttg ttgagcacac 37560 tggatttgat tctgcggttg ccatatttaa atctgtttat tgggggaaag attggatagc 37620 tggcatggat gttgttccgt ggacttctgg cggcgctgaa acacatcttt atgttaaggg 37680 cgctgaattt atttttgata gtgctggaaa tggtagcgca tcaaattggg ttagcaggtc 37740 tgacattaga ttaaaagcac atctaaaaga gatcgaaacg gcatccgaca aaattgatta 37800 tctaactggt tatacttact acaagcgcaa caatctaatt gaagatgaaa acagcgttta 37860 tagtattgag gctggattga tcgcacaaga tgttgaaagg gttttgccgg aagcggttca 37920 ttctttgaat aacgatggtc agctagaccc aaaaggcgag gcaatcaaag gcattaacta 37980 taatggtgtt gtcgcacttc ttgttaacgc attcaaagag caaaaagcaa agattgataa 38040 tcaacaggag gaaattaatg tattacgcaa tgagttatat gaactgaaaa accttgtaaa 38100 atcaatgctt aacggaaatg ctccaacaat tacagaacta ccgtaatata agtggagtgc 38160 aatctgtcac tgaaaaatat tgctgcgaaa caagcctgaa cattgattat cctagcaacg 38220 caacgcgtgt tctgctatgt taacagttcg ctgattctta ggctgttctt atgtacaata 38280 tgaaaacgca aaatgcaaaa tgcaaaatgc aagaggttcg ttttgcaccc tctcaaaaaa 38340 aaaaaaaaga ttaataaaat aaaggaattg gtaaaacaac ttattgctaa ataaaagaaa 38400 accccagtgg attgtccact ggggtttatt attatctact acctgcaata atacctaagt 38460 tatatactgc tagcatggct ttaagcatag gatcttgaga ttcagtgcaa agtgcgataa 38520 acccttcttc atcccactgt tctactacac cagatacatc aactctcagt actattttct 38580 caggttcaac gtatttaacg tcaatcttgt aaaagttaga ttgatctaca tctactccta 38640 ccatattagg gtcagtaaaa ttatttactt cctgagcttc caaagccgcc ttctccacga 38700 tcagtctcct ttagttcgtc aacgatttca aaatgatggg ttgagtagtg tggtagtacc 38760 actagctgac aaagtctctc gaaattctcc agagtttgca tttcagaacc gtagttaaac 38820 aggttcatct taatagtacc acggtagtct gagtcgatca ctcctgcggt atttgcgatc 38880 atcagtttac gcttacctaa agagctgcga ggaactacca aaccaaacca acctcgcgga 38940 atttccaccg cgacaccggt gtcgatcata agagatttgc ctggtgcaat agcacgtaaa 39000 tctgctgcaa ggttagtacc gaagaatgca cgcagatcca tacctgcggc atcatcagaa 39060 ccaatcttcg gcatgcaatc tggatgagtt agtttaattt taatcattgt cctgcgatct 39120 ccaaaatatc tttcgtaaac ttatctaata cgtcttgacc tacagcagca atagcatcta 39180 cacaataggt cggtaaatca accagaatca agtttcggaa aagtaactcc tccgatgcgt 39240 tcagattctg tatatatttc tgtttcccag gcagaggtag ctgatcaata atatccagaa 39300 cattaccaaa ttcacggata atattataac cacgttttgc accaatgcct tcaacgccac 39360 ggatgttatc accgagatct cccatgattg ctttcagaga gataaactga tctacatcgt 39420 caacattatg atgctcatac atatcacgaa gatggtactc acggcgtgta gtaaaggaga 39480 agcgagaaac ccgatcagtt aatagagtat cccagtcacc atcagtagag atcagccaaa 39540 cgtgatcata taaatgacca atgagcttaa caatataagc tgccatatca tctgcttcta 39600 caccacgaat agtgaaggtt gggaatgtgg tttcacacaa ctcgaaagca tcacgaaggt 39660 attcaaagaa ctgttcatcc agtgctttct cttcttctgt acgttgagca tacttttcat 39720 cacggttccc tttgtaatca ggaagatgct ctaaacggaa tacagacttt cctttatcac 39780 cgagaacgat tgtagttcta gctgaatagg attttgctag agattgaatt gtagaaacat 39840 aacttgatgc gaatggtttc ttactattat tgtgtttgaa gcggaagccc aagttagttc 39900 catcaacaat cattaggttg cgacgagaag ccagctcagc ttcttcctct tcaataaatt 39960 ttccccagga tttactcatt atttaattaa gtcctcaaca gatgcgtgat gtaaccacgg 40020 ttcgaataac ccgattacga tttccatgtc tttcttattt aacaccatat gggtacgact 40080 cattaagttg tccaccatcg ggtctgagct atccaaagca attaaccact gtcctctgtc 40140 tttcttgaat attaaagctg gtttggagtt catctgttca ccttcacggg aacactgctg 40200 ccaccacttc tctagagtgg attcaccaac attaaataaa ttacttgata tgctatcatc 40260 tttataccac ttaacttcga agcagtattt actaatgtgt ccgctttgtg gtggaaggta 40320 gatgtcaccc ttcagtccat ggctctgacc aaaagcacca gagcctggaa cacgttccca 40380 ctcaagacct gtacgttcac gcagtatatc tcttacctga tattcaccac gtttaccttt 40440 ctctctacta tctacggcca tgttttattc taagtaggaa aatccttctg catctttttt 40500 gacagtaatc ttatgggcta atggatgcgt atgcccatga gaaacaatga tagagttcag 40560 actatcttcc tcatttaata gttcaacaag agtatcaagt cctttcgtat caataaagct 40620 aataacttcg tcaaggaata gaagattaat attaactttg ctgatagatg ttaacagcat 40680 tcgaattgcc aacagagttg ctaggttaat tctactctgc tgaccagtag agcagttctc 40740 catactggta cggtttccat cattgaagat tactacttgt aatttagttt catcaagctc 40800 aaatccaagt gcgaacttac caccagtcat aatagaaaga tatttattaa tcaattcctc 40860 aaatactttc acactatgct ctagtttata tcctaccaga tttttcaatg cagctatcag 40920 aatatcaaga tcagtgacag cttctgatac tccatccagt ttggacgtta tctcagacat 40980 ttctgcctca gctttctcaa tctgttctag tttcgcttta tactttgcat tggctaattc 41040 gacatttgca ttatgttctt tggcaattgc aactttagaa cgtccatctg cgatttcctg 41100 ttctaattgt cggatttgca cctgtaggat ttgcacattg agttcttcaa aagaagcatc 41160 actcattgaa tttttgaatt cgtctctagc tgctactgcc ttatccaaag catccttagc 41220 tctagtatac gcagtgtact cgacttgttc tttcttcaac tgctctagcg tagcttcaag 41280 agattgcttc tctttaaata gaggatcata ctctgttcta gccatatcca ttgctttttg 41340 agcagcagtt gtatcaagat gagtgccaca agtaggacat tcagtatttg aagcctcttg 41400 cttgaacttc tgataacgtt tcttgacttc tcccgcacgt gaggtaacaa tcgttaggtc 41460 acgcgtaaca cttgagatct cttcagattg gtcagtgggc gcagggaaat ttttgaaagg 41520 ctcgaaagat tgttcggcaa cttgtacagc tttgtccaaa ttacggagtt tagtaatatt 41580 agcctcttgt gacttggcta atgccgcctt aattttcgat tcagtaagtt cttgtgccaa 41640 tggttcttca tcaaactctg gaacttcaac tggttcttgt aaacttccga gattgccttt 41700 tccattgagg atcttcgtaa ttacggccat ctggccttgc aagttactta acgtacttgc 41760 cacttcttta cgatcagcct taatagtttc tgacatctct ttatactgtt cctgattgaa 41820 cagattaaca agaaaagctt tacgtgtcgc atcagttgct ttgaggaagt ctagattgga 41880 accgacagat tgatagatca gtttagtgaa cgtttggaag tcaccgccca taatctcttc 41940 aatcatcttg tatgtttgag ttgctgtgtg tccactgata tcttctccat tcttaatcag 42000 tataacctta gcagttgatt taactacttt atgcaactca tactcatctt catctttcga 42060 gaagtaagcg tgcatatcgt actctttctt cggagaaccc caagagaata gggcgtcctt 42120 cttgatacca cgtgagttct tgttgtagaa caattcctcg ataacagtag caatagtgga 42180 cttccctaga ccgtttcctc cgattaactg agtaactgga ttcttatcga aatgaactac 42240 aatatcctta ccgtaagaca taacgttgct gaattttagt gtcttaatta taatctttga 42300 catatttagc agctctagct aagattctat caatgtcgtc ttgagacagc ttttcaactt 42360 cacggaagta aagttcaagt tcgcctaaca tatcaagatc aacaagattt aacttagcat 42420 ctttagtaac tcgatggtta atctttttat ctaagagatc ggaatcttta atagacttta 42480 actgaacgac gtcaccagtg acttcataaa ttacacggtc atagtcacta ggctccattt 42540 cctcaccaac tccgattgtt ttacgaatca gctgtggtaa gtcaccgagt tcaatccatt 42600 ctacttttaa tgtgtcggta tcaatgataa aacaaccatt tgtacctttt gtgcgttctc 42660 tgtggaacga tgtagttaat ggagatcctg gataaagaag tctagtagaa ccaacagttt 42720 gactattagt ataagaatgt aaatcgccag caattacagt gtcataacag ttatacctcg 42780 ttagatcaat ctctggtttt acatgtggag ggatttcacc acgaacatgc gtgaaacata 42840 gtttggctc agatggtttc cactttggtt tgtggatctc atcataaggg acaatatcaa 42900 attcaggaga acgatatggt ttagtaatga cttcccaatt tccgtttgtt actttattaa 42960 taactcctgc atagtgatac agacatgaaa tagttttagt taacatttca tggttccctg 43020 tgaagatctt gcctggatgg tcaagtcttg acatgaactg ttcaagaagt tctatttctt 43080 ccgacgatgg gtcggcaaca tcaagaatat caccaccagc aatatgcaga tcacagttat 43140 gattatggaa tatgtcattt aaccgttctc ctagcatcag gaagcgacgc ttctgccatt 43200 cctttggaac tttatcttgt cctaatttga tatgatgatc agcactaaat agtattctca 43260 taagttaaaa agaaagggag ccgtagctcc ccttggttta ttagtcgtcc agatcgctag 43320 cagcttcagc gtcgatgcct ttctgagaac cagcatctcc accagactta gcgtcaccgc 43380 cttcttgctt ctcacgacct tccatgaagg cgagaatagc ttctttctgc tcttcatagg 43440 acggaaccgg gtaggtttct tccagagaag gaactttttc gaacttgatg aactcaccag 43500 tatcatcaca catagcttca ccgaggatat caacatcagc ggcgtgcagt ttagactcag 43560 cactggtagg atctttcagt ttcatctgga actgcattgc tgcgatctgc tgtacatcgt 43620 actcagtatc gaagccttta cctttcttct cgatagaaat atcgatatcg aatggatctg 43680 ccagttcaag ctgcttcatg atagactgga tgcctttcag gatcgtagct ttaacttcca 43740 taactttcag tttgttatca gaacggtcga taacgaaagc gatgtagttt ttcttcggtt 43800 tcagcggaac acgattacca tctttgtcca gctctttctc gaagaaaccc atttcgtgaa 43860 tcgggtcagc tttaccgcga acgaagctct ctttagtacg gttgaaacgc aggcactcaa 43920 acggagctgc attaccatct ttgttagtca gccagtatac ataacgtgga agaacaccag 43980 aaacgatacg aacacgagtt acaccgttgt tgaatttcag gaagtcgatt ttatcgttag 44040 aaccgccagt agtttcgccc caagacttag ccatatttta tttcctcttt aaagattatc 44100 ttcgatttat tgattgcaat tagtgggtta gtgtcgatta cagaacgtgg tatccatatg 44160 gggacatatt gcatgtccaa actcggatcg tttgtaaact tatattcggc ataatttcgt 44220 agacttagaa ttcctagata ttctgccagt tgtctattag acaatttgta agggttatca 44280 acaatagttg attcattcaa gatgaatgaa gaaccgacca gtagttggtg agcatcaggc 44340 tctgcaagca ttctattaaa tagcttgaca atcaggtcgg agtttcctct agctaagagg 44400 taaattctct cgtaatcaaa gaatttcatt tttgtctctc atcaatttat gtatatatta 44460 tacattaaat cgggaggatt tgacaagtac aatttttatt tttctgcttc ggactttctt 44520 atcaatccga aactttctct ctcaaattta tgtatatatt atacaataat tatgagtagt 44580 tgccaaatgc aattttagct aattgtactg ctttctccgg agtcatagta atagttttcc 44640 atgcattatt gcgatacacg gccatacgtc cagaagcctg tcttaaacca gtacctcctt 44700 tcataattaa atctacaaca atcggatcta gctttccgtc aacaatacgt tgaacgcggc 44760 ctgctaactg ttctatgaga gattcattat taatgagact tcccattatt aaacaagaca 44820 gctcgttaag agaaatacct tctgagaaga tactctgagc tgctgcaagc acacagggtc 44880 cgccctccgc tacatctttc tggattctca atcggtcatc caaaggagtt gcacctataa 44940 tctcataggt tgtaacacca cgttgtgcga gagcttctaa tattgtttgg attagttccg 45000 tcctatcact tacaaagaga accttatgcc ccatagacac atataaatgt gctaggttta 45060 taatagtttc ccgatattca ggatgattat atacatcatt agcacgtagt gcccatggta 45120 cgttctggtt tcctgataac tcgacaggta cggagtacct atggattgtg ggtggaacag 45180 tgttattaac agggggacta aagatcttat atccaaagaa atctttgaac ataacttgta 45240 gcccatcttt acgttttaat gtacctgata gaccgatctt gtagcgagca catgagatct 45300 ctaggaaatt agtgaaagtt gtagctacac agtggtgaac ttcatcaact ataacagtac 45360 cgaatacctt agaaagatta ttcgcatgtt tgttcactgt ttgtatatta ctgaccacga 45420 ttggtggatc aatgttatac tttccagaac ctataatacc cggctcgaat ccaaaccatt 45480 tacgaacttc tgcagcccac atttcacgaa tggatgtgtt agtgcagatt accagagttt 45540 tctggccaaa cttatatgca agagctagag ctaggatagt tttaccaaat ccaggcttac 45600 cattaataat acaggtatca tcgcactctt catatatagg aagctgatct tcttcacgta 45660 gcttgaactg aggttctggt atttctaccg gagctaatgt gcgtttatca actaattcgt 45720 attttactcc cttagcatct agtaaatcca ggcgcgtaat gggaatccac ttaatctctt 45780 tagcaacaac accactattc ttatacataa tagggtattt actagtcatt gtctcgatgt 45840 gataagtggt ttgcttacta caataatccc agagttcatc atcgggcttg aaataggctt 45900 tattagatat aacaaccttc ataattttat tctaagtcta ggaatttcag gctcctcttg 45960 atggacttgg taaataacag ggctattatt taccagaata tagcttatat aagctggaac 46020 ataggatagc acgaatggat acgggacttt agccacatag cattgatact ttccattata 46080 aattcttgca gaatgcagaa ctttagacgt gataacatca tagaacgtag ttttcttcca 46140 gttaatgaga tttccatcag aatctatgaa ctggctacgc tttgacccaa ctaattggga 46200 caacattgat attctaccac gaataggata gagcttataa ggtaattctt tccgcttctc 46260 aaataaggct aacctacgtt gagagaatgt gccaggcaac tccctgttat ctagcacata 46320 tttattatat cttgttgtaa ttacggaata atcaccttcc tgctcaattg atacaaacgc 46380 ccgtaaagca tatacgggca atttgaaatc aagcacctaa aatccttttc acattatcca 46440 gatctttaca tacagcaata aacttatcat ccttgtactt gctgtggtct ggatgctctt 46500 tatccattgc agctaacttc ttatactcga aatctgcatc gagtaataca cccttaacat 46560 aacgagtata ctcatcatca tcaatacaag cgattgatgg gtgctgtttc ttcatcttac 46620 cacaagagta gtcacgagaa ccacctttct cagaatcaga atcaataccg attgggcagc 46680 cagggatact aataccacga tccttctgga tgttacgaat cagaatctcg ttatattgat 46740 caatcagatc ttcacgtaca atagcaacca cggagtcgtg aaccaacatg acgatcttca 46800 tctcttttcc tagacccagg gataggattt cttcatcagc atcaatagca cctaacagca 46860 ggctatcaga agatgcagac tgaatgattg cgttaaatcc agaacggatt tcttcaccct 46920 gaacaccacg atcttcggaa tggatgttgt gcagacgacg cttacgacca aagtgactat 46980 agatgaatcc aaagttcttg atctgatcgt ggcacttatc aatccaacgc ttaagctgag 47040 ggaactgacc gaagtaagtt tcgatataat ccttcgcatc tgcaacggta cattcaacaa 47100 acggttcgcc tgtcttagca gcctgttcca gaagagcttc gttaacagaa tgagctactt 47160 tagctgggcc agaaccatac agaataccga aggtaattgc cttagcagcc tgacgcagag 47220 ctgggaacag cttcttaaca tcacgtggtt cacaggtcag tttaaatacc atgtgtgcga 47280 tgtttgagtg gaagtcaggg tatttatcag gctcattctt catgttgata aatacttgtt 47340 gcatgtttct gtcaccagac agtacagcag cgtaataaac ttccgcagtt gttaagtccc 47400 atgcgattac acgatatcca gggggagcta ctacacaacc cttaataata gattcatcac 47460 gtggtaactg ttgcaggttt agcttaccag aagaactcag acgaccagaa gtagtcatgt 47520 gttcgtggaa accggtacga atgcaaccat ctgcatcgat gctcagaaga attttctcaa 47580 cataagtaga gatcagctta gtcagcttac gaatctctag cagagttttc gcaatcgggt 47640 gctgagtagc caattcattc agagcttctg cgtttgtaga atctgcacct gtatctgtca 47700 gtttaccagt tggagtcaga ccaacataat caaacagaag aacacgaagt tgcttaacag 47760 agttcgggtt aaatgcttcg ttctgatctt tctctagctg aacgacttct ggataagtat 47820 acagcttttc acgagccttg ttcaggttgt gagttaactg atactgagct tctttcaagc 47880 gatcaacaga aattggtaca ccacgatctt caacacgttg caggaatacg caaccaggca 47940 tcagaacatc atagtataga ctgcgaagtt tttcattctt ctcaattttc ggtaagaaga 48000 agttatgcag acgcagagta gcatcggtat cttttgcagc ataaggccac ataatatcaa 48060 atggaatcag atcatagctg aaatcttctt tcttgatctt atgtgctttg caataatcat 48120 ccttgaactt atccagttca aagtcgtaat cacccatgtc agtatacttc atagccagag 48180 atttcaagcc gtgagtacca cgacgttcat cgagaacata gtgttgtaac atggtatcat 48240 gaagtctacg ctctttatgt gatttctcga aggaaagtcc tagatgatag gagtaaaagt 48300 gcatatcgaa cttcaagtta tgaaaaacga tctgatgatt cttgctatcc agaattttct 48360 ggagataatg tacagctact tcggtgatgc tatcagagtc aatatagaca ccctgatact 48420 cttggtgaga catagaaaca ccaagaagat aaccatcacg agcatacaga gctgaggttt 48480 cggagtcgaa tgcgataggt ccgataacca tgttatacac catcttgata tattcttcag 48540 cctcatctgg atcagtaata gggcgataat caccagcttt tgcaatcttc tcacgaccat 48600 taataatgtc atgaatactt tctacagttg catcgaaaac tggtttcatt tcaggtttaa 48660 agtgtaactg agctggacta atacttgcaa tccaattggc atagccgcca tgctctacac 48720 gtttaccagt ataatcaccg atacctttct tacctgcgaa atacaggaaa ggttcagcac 48780 caactagtac aacaaaatcg taatcgttcg gatcgaacgg gttatctgga gttccgattg 48840 taatatgctt tttcagcaaa cggccagata acttctcgtt acacatatgg aatacatcaa 48900 cttcctcacc ataaagctgg aagtgtttat cataacgagt gttatttaga gctttatcaa 48960 ctactgcaat tttcaaattt agtctccttt tggtaagtaa gtataacttc agtgtttctt 49020 ctctaactta ccaatatatt ataccaaatc tttgagcgat tcggcaacta aaatttcaat 49080 acgttgagcc aacatatcga tctcttcttt atttaaatca cctgggtctt tgccttctgg 49140 aagaaggaag ttaccaacta caggtgttaa acgcgttttt gtacgaatta gcttcgctag 49200 tgcttgtgcg gctttattac cagaggcatc attatctaat aggataacta ctactcttac 49260 tccagcaata atgtaaggac tgaacttatc ggcaatgtta tcagatgtaa actgatgtgt 49320 accaaagcag caagaagcat aatccacacc gttatcttct agatttaaca tatcaaagat 49380 accttcaact agaacaagaa ctggtgtatt atatcgtaca ggaaaaatcg gtggtgaaac 49440 ttgttttggt tttactaagt atttaggagg ggcagaactg tttatagaac gtcccaggaa 49500 taggatattg cgtccaacag catctgttat tgggaataca attctgcctt cccagtctgc 49560 ttgatgttgg aaagcaaaat atttcttcaa ggtcttagaa ctgatacctc ggaaatcacc 49620 ttcgaaaagg taagcagatt caggaattgc aagattcgta gatccattcc taatctctga 49680 aatcttttta cgtacttgtg acaatcttgg tgactgtctg tactgagtct cattaaaata 49740 atggtaaatg ctcggtattc ctctaccgaa gccacaactc aagcagtgca taatacctga 49800 ttcaggatca atacgcaaac ttgggtgttt atcatcatga tctggattga gacaacagat 49860 gaggatgtcc ccacctgtgt ctttgtattc aatgcctttc agatcaagta gttctgttat 49920 tctactcata tatcgctggc ctgttcacct gttgaatttt cagctttgtc tttcttagca 49980 cgtttaggtt gagctggttt atccttttca ataggaataa cgaactcagc ttccatttgc 50040 gt; ttaggcataa acttcacgct atcagaagaa cgagtcttaa cgaagtcaaa cataatagca 50160 ccttgaccat tatcagcttt tgcagcatta agattagcag ccatatctgc ggaatcaaga 50220 atccctttag acatacgcgt tctaccatct tggtcaatct ggtaaggagc aacaccagcc 50280 acattatgtt tctggcagat agatttgaaa gatgaactaa caaccatctg ttctttccag 50340 tcatacatgt caatggtctt tgaatctgga agtcttgttt ggttaatgta gtctagaaga 50400 gctacagtaa ccttatcacc atacttagca actagtttat ttaattctac gtcaaccgtc 50460 gtaatagaca gttcaggatc gtaaactatg atcataggag tgtgaagctc atagccttca 50520 aggagtttac tttccatatc atagaagtcg ctcattttag ccatagtgta ctgcttaacg 50580 aagttatcga aaagctcttc accaccatta aacatcctag ccctagttct ggctaatctc 50640 aacagagcag caccttcaag agtgttatta cgcattgcta atgcagatac cctagctaac 50700 atagccagat tacgtctaaa cacttcgtgc tctttcatct caattgagaa atatggagca 50760 atgtctccat tcaaatattg ctgaacctgt atgtttgaac agataatgga tttaccagta 50820 ccacgccaac caccaagcag taacgtttct gtgcgagcta aaccaatttg agcgtcgaac 50880 tcattacaaa taccaagagc gatcaagttc agtttggtat cttcttctct ctggaaaata 50940 cgcattgtgt ctgcgttgaa tacctttcca gtattcgtta ctttctcttc taattttaag 51000 tgaagggagg caactcggtt gagaatttct ccctgatcca gcattgttaa atcttgaagc 51060 acgtctgttt ctagaagttt caggaataga tcctgcgtat attcagcttc taggacttca 51120 agtgcctgtt ccatgctcac ttctggaatt tgagtgttag ctaagacgac aagagcttga 51180 gaaaggcggg cgttcctatt ggcctcaagc atcagtgcgt caatggacgg cattgcgtta 51240 tatttcttat aataattctg gacggcttgg taaattgagg agaaagcgtc attaaaatga 51300 tctttatgca gttttgagaa tgtttccaat gctatttgct tctgttcaga agcgagaagc 51360 atcttcaaca ctacagcttg cacgttaaac aaggtcattc tcctttgcac gctttcgtgc 51420 cttctaaatg caaaaagggg aaggagcata gccccctccc cttaggttta atttaccaga 51480 ttattcagca gccgcagctt ttgcatccag tttagcacgc ttagcggcac catcatagtc 51540 cttagcaacc agaccacgac gagacagcat agatttaaca ccgcgctcag acttaccagt 51600 tttctcagcg atctcagcaa cagtcatgtt agccagatcc agaccttcta acagatcttc 51660 acgagtttta gcacttgaag tttcctgtac cggcatagca gcgatacgac cttcacgaag 51720 cagactcaga gctttaccac ggatctgctt aatattgcga ccgaagtgag cagcgatagc 51780 ttcaatagta gcaccagcaa caacctgatt aacgaaatca gtttcttcgt caggagtgaa 51840 ggaacgaaca gcagcagctt tttcggttgg cttaacagaa gcagtcattt ccagactcag 51900 gatcttaccc tgtacctgct tagcaccgaa ctgaccacca gctacagcag cagcgatttc 51960 agcgtaagta tactgaccag catgagcatt caggaaatca accagttcag cttcctgctc 52020 aggagtccac ggggatttct gtacttcgtt agctttctgt acttcgaaac cttctttacg 52080 cagcttagat ccaacagagc gagcggtaac gtctttacca gtctctgtag ccagttcagc 52140 agcgatagca gctacttgtt cttgagagat tacagcaaca cccagagcag tggcttttgc 52200 tttcagagac tcggttacac cttctacgtt ccagttcagt ttagacatta ttatttttct 52260 ccaatagttc tttaatcgac aggatttcta tcccattcgt ttcggctttc ttataagatg 52320 aggaagaacg ttttgattca tcctcacaga ttaggtattt gacatctttg gtaacggact 52380 tcttaaccgt atatcctaga ctttctaaat agtttgttgc atccgttcga tttgcaaaat 52440 cttgcaaaga tccggtaata cagaccgtga ttccatttgg ttgggcaact aaatcatccg 52500 taatgatgat atcagcttta gtgcctcgaa taccagttga gaatttccac ggtaattcaa 52560 taacatcctt gccttgtggg gaatttagcc aggttttgta attttctcca gccttgccgt 52620 cagccttcac atcgtggaag ctagtgcaat tttgggacag tttctttgct gcaacctctc 52680 caataagagg aattcctaaa gaaccgagaa ctgaaccaaa gtcaatgtct ccgcgaacct 52740 tagtatttaa ctcgctaatc aacttagcgg caaccttgct acccacagct ccgaccaaat 52800 cttcttcggt caggtaaaac agttctgaga tcttagtcag ctccagcttc tcaatagttt 52860 tcgggccaaa gccctttaac ttcatttttg tgcagaagtt ctcgattaac ttacttgatt 52920 gcgctggaca gttggactta ttcctacaga ataattgccc gttgacaaga tctagcttag 52980 aaccacaaga gggacattgt gttggaattt cgattttcat caaatttctt ccttatcaat 53040 ttatataaat attatagcaa gtatttaagc atttagcaac tacaatttta actaactttg 53100 cttaccctcg ccataacttt ctatccccca actgaatgat aatagtatat acctaagcgg 53160 cgaaaatgtc aataaccact ttataattcc gacatggtaa acgggtagca aagggttacg 53220 aattagtcgt atactcgctc tactatacaa gggattacac caccagcacg aatcactcga 53280 atctggcaac cgatctctaa atcgagagcg ttaatatagt caacgttatt aagagttgct 53340 ttaacaattg tggcatcatc aataatcacc ggctcgaaat aaccaactgg agttacttta 53400 ccagaagccc ctacttgcca ttcaacttta acaagtgtgg taatttcgcc ttcttcatcc 53460 tctttaatag caaatgcacc acgaggaaat ttattagtcc aaccttcacg gaagaattta 53520 ttgttgtcgt taatacgaac aactttaccg tccgtcggaa tccatttaaa aaaggaacga 53580 acattaacaa ctgtcaagaa gttctcattc tctagccaaa gcatatcttt taaataggct 53640 tctgtaatac cgactgtttc cgctgaacac tggataccat atgcaacgaa gattaaacca 53700 ccttcaccaa tacgttgcat gaaatcatca ctatctttca ggttaatagc acccgaagcg 53760 tagttacgtt tgttttctac ttctttagta atgagaactt caccagtgat ttgagttggt 53820 actttttggg aaatagtttt gggaatgttc agcattttaa cattatgcgt tacatcattc 53880 cctagaatac cgttaccacg agttaacgct tgaacaaatt caccattaat atataataga 53940 gaaatcgcac aaccatctaa cttatcagtt tcaacctgac ctaatgggtt aaatggagct 54000 ttatcaccac gatcgtaata gactttctgt aaagaataca tacgatacaa atgagggata 54060 tcacctttcg gtccgatttc ttcctctagt gggaatcttt taatcagacg atcatattct 54120 tcatcggaaa taagactcat tccctggtag tacgcttctt gacaacgttt aataaagtct 54180 tttacattat tcatcatctg cgatttccat tttgagttct ttactatact gcgcagccat 54240 accagctaaa tcagcagctt ctttcaacca cgcatctttg tacgctttct ggtttggagt 54300 gccgatacta tcctggtaat tgattcttgc tatggagagc aaattaagtt ttgcttgaat 54360 ctttggaagt gttattgtca ttgccataat tgatcctctc atcaatttat ataaatatta 54420 tatatgaaaa ctgagtcgaa agcaagtaca tttataaaga aaaagccaag aactagacat 54480 tcttggcttt gagttcttca gctcgtttct gtacttcatg caagacctct gattcactga 54540 ggatttgcgt aaaagcgtag aatagctgtg atgtagtttc tagggtataa ggaaaagaga 54600 agccggattt cgttggaaac cactctccag taatatcttg gagccagtaa cgaattccca 54660 tgtatagatt cccacgaaat tcagatacgg ttagtcttac ttgttccccc tccttctccc 54720 aaagcatgat ggactggtcg tcaacgtgtc cttcatagtt ttgatttacc tgatcgctca 54780 tttacatatc ccaaatacac aaaaagcccc atataggggc taattggttt cttatcggcc 54840 tactggagaa gcacgatcta actctgactg gaggctcgtc acacggcgaa ctttcgcaac 54900 aggaatgaaa cggaagctat cgttagtacg agagaaaacg aggatttcat cttctttcgg 54960 cttacggatg cgctcacgtt taatacgctc agccaggtat ttatcctgag ctggatcaaa 55020 ttccatcgta ccctgaaggt acgtggttcc tttctgacga agtttttcgt aatgaagata 55080 aaactcacca aatttttcgc attgagcaat aatttcagct ttattcataa ctgtattcct 55140 tgtgttggtg gatttatcta agaattactt agtgatagct cggatagctt ctgccagatg 55200 agcagctgct ttaccggtca gcttgtcgat gattgcgtcg tccaggaagt ccggggctaa 55260 gccagcatca gagaatgcag agcgaagatc ggagtgagcc tgagctttgg aagtacgaga 55320 accaccagaa gctttttctc cagtagatgc gcttgattta gaagtagagc cagcagcttt 55380 cttgatatac agacctgctt tggtcagctt catacggaaa ccgttcggag ttacaccatt 55440 ttcctgagca atttcgctaa caatttccat gctaacgccc ggacgctcat cttctgggaa 55500 ttgctccata cgagcaacgt agtcggaaga cattttttcg aacagttcat cagtccactg 55560 agttggagta gtcatatttt tcgatttcct taattaagaa ttaaacagaa tagttctttc 55620 aaactatgag aatattatat caagaattac aggattaagc aactgaaatt tttcagtaac 55680 tgctacacat ctagttcaac gatctcccaa tcgtcggcca gggcttcttc tagggttagt 55740 tcacacactt catcacttgg gaataatgat gtatcaaaag gcatccacac gtactggcgt 55800 acggtattgc tatctacaaa gaatttcagg attactgtgt aatctttatt attttcccaa 55860 ctagttcgct ggagatattt tacgtctcca gcttttaaca gatcagttag tttactcacg 55920 acgttttcct ttctctttat ctagtttcag ggcagcttta acagcttcat taaccaacgt 55980 gataacttct tcacgactcc acttaaagcc cagagcctta acatcaacac ccagcttttc 56040 cagatgttta acagaagcta attcgtagtg catatagtga acgttctgtt gtttaccttc 56100 tgataacaac caaacacgat agcaaccaac tggattagtc attgctttct tgatttcacc 56160 aatacactgg tagccaggaa cccaaacaag ttctccaact tcaaattctt ctgccattgc 56220 atcatcagga atcagcggtg gattcaatgg atcaacaata tcgtttaaac gaagcagagc 56280 accataacgt tccagaacag atttaatcat cgctacagaa cgataattac gatctgcgat 56340 ttcttcaaaa gaatcaccag aaagatactg ttcaattacg tttgcaagct caaccccctc 56400 aatcagtgtt ccgcgcttct tctttttcat ttccgcaact tgaacctgac gatctctcca 56460 ttcttcgatc attcgttcca tagttgggtt agaagaaaca ccgagcatct cacatgctgc 56520 tttcttagta ccaccattct ccagatgttc aataaccttt ttaaaggttt catcagggat 56580 ttcatggata agtttctttc tacgagaacc agccatattt aaactcctct ctcaaattta 56640 tgaatctatt atacagaaaa tttttcctga aagcaaataa aatttttatg attctttgag 56700 caaagaagca agttcagcat agactttatc taactcaaag ccaatgcgaa gagacacacg 56760 agtcttccgt tgtttaagta accatatgga gacattaggg atgccagcga gacgaggtgg 56820 tacaacccta catagtttct ccgcagtatc gtaaacagaa gttttacgtt cttttactgg 56880 aaacttcctt gtttcccact ttttattctt cactgtcatt tctagctccc aattcagcaa 56940 gagcttcctc gtgcatctga cgttcttcgt ctgtcataaa cttaactgga atcggcatac 57000 attcgatctt acagtaagtt ctgtaccagt ccacaatctt ttccgtatcc agatctttac 57060 cgatacccat catgccaaga tacatacgag catattttgg gttagcgctc tgacctgtct 57120 taaggaagaa atccttcttc ttattcttaa gagcttcgat aaacggtttg attgttacgt 57180 ttgaacactt tttaatatct tcccagaaca tgtcgtgcat cttgtggaaa aatgcagctc 57240 gatcattcgg tttatccttg atatactgtt caacatgttc cactgttaca tctttaatag 57300 aatccagatg ataatagcgg accagaagta acttggcttc ataggcatct gcatgacgtg 57360 gtgcacaata ttcaggagtt gagtgcaaaa tagctttgat tcgctcttct gtatactctt 57420 ttcctgcacc ttcgatagtt ccccagcttt cactgaaaat atcaatagtt gcaccttgat 57480 cgagaagata ataccgcaaa gtactggtat agtttctaga ttccagctca cgacgacgtg 57540 cgtacagttg ttgggctaac tcaatccaac cttcgtcaat attatattgc tcggaacccg 57600 cagaaaatcc actcgtagat ttatcaataa aataataaat gttctgagca ccacggtcgc 57660 gcctgattgc ttgaaaacgc atgtttggcg cttggtttga ggttctagtg attacgaata 57720 cgttatcaaa atagttaaag tcaacaccac tcgttacaga tggactacat aataaacaat 57780 caatctgttg atcaattagc tcattagttg tataatccag aatacgtcgg atatctacat 57840 cagacgtaga gtttgagtga atttctttaa ctaacgcacc agtattacga cgtaatgcca 57900 ttcccttctc attcagttca tccgggccac agtcagatac taggattgac ttctcaccca 57960 tctctagaga tgtttgaagt gcaacccaaa tactggattc gtcagggaat tcataagcgt 58020 gagctttcgc cagcatttta cggtgatgat tatagaatgc aactggttta tcaaaatcaa 58080 tcaaagaacc ataggcttca atcgtttctg cactgatatc accatcagat aagatcaccg 58140 ttttagcagt aataagaata tcgcgaagaa cctgtataca ttcacgacgg tttttaacaa 58200 ccggagcaaa taacagatca ttcattactg cgtcacattc atcaataaag atcgtatcaa 58260 tctgacccac aaagctcttg aacttatgga tagagtgaat agttgaagac atacgatcaa 58320 tggcaccacg tttgaagttc agcatatcaa ctgacttatc atactgacca gcaccgaatt 58380 tcttcgcgtt tgaagataca agtgccctag tatttgtgat tgcaataaag tttcctggaa 58440 gaacacctgc atctagccac ttagttactg cggtagtttt acccgtacca agacttgcct 58500 taacaaatgt taaatgtcct tttggtggta caatgttaat tttcaggtat ggaacttctg 58560 gtggagaatc tgtgtctaga cgttttaatg gtacaccttt taagtttggt ggaatctcac 58620 gtttggagtt atttacaaag gctttgagag cctgcttacg accgttatta aagtaatctt 58680 gaatattacg actattatct ttagttgcaa tatattcaga taaagctggt ttaatctcac 58740 gttctaacca tgtaaagtca acaccatctt ctaacgctct gtggtaaagt tttggaataa 58800 tacgcaggta caccccatcc tcagcttctt ctagttcgct gatggtttct tccactttat 58860 cagatgcagc cttcttgcct ttaatttggt caagtaagga gtagaattct tctttaaact 58920 cacctctagt tgtttcaaag tcaactaggt tattgggtag attcactttc gaacccttaa 58980 catatacaag acgtggttta ttctccgctt taaacggatc gactatacca tcatcaaata 59040 agggatctgc aaaatagtga agctgaacag aagaataata agctaagtca gcaatatcaa 59100 agccatactt ttgcttacta ctgtcattga tagatgtaaa taagaacttt aactgtccct 59160 gcgttacctt gacattagat tctagcatta agtgcattcg gatacctggt tttaatccag 59220 ccgaagacga tgcatgggca ataaaccctg catcaagagg aaacatatcc tcactaatac 59280 tattcaacat cttaataata tgacgtgcca tgccaacaag attaaacttg tcatagccac 59340 cagtatctac aataccatcc acgtccatag caataatatg actaggattg gatacgttaa 59400 agtttcctct ggtacgacgc acattatctt ttggagctag acacttaccc cgaatagcta 59460 caatatgcgg gtctttggta agacgacgca tgataggtaa catttcagca aggttttgcg 59520 ggtcaagttc ttcaagtaca tcaaatttga agggcatgga agctggttta ccttctggat 59580 gctctttaga gaatcgcttt gcaaaggtgt aatcttccac tttgatctct ttccagatgc 59640 cagtggcttt atctctggaa aatcctgcgt gaccttgtag gattgagaac acaacaatac 59700 tcctatgttg gttattcgga tatctttctt taggactatt caaaagaata gaacaactat 59760 tactaccatt tacacaaaca ttaatcacca tcaagcagta tgaataagtt atcatacctc 59820 agtgctgcgc tctcttaact cttatccaga tcagaaccta ggtcattggc cgctaagccg 59880 attctgattg ccctcactat cctattggta ggtggctact ggacgtgtca ccattcgctg 59940 ctcttttcgt tcgcaccccc atataaagct ctgcctatat aacgagctaa atatgcacgg 60000 tttggattta cctttccgaa ttcagggtac gcagatcact taattgataa ttaactaatg 60060 cagtataaac actagtaata gttaaaacaa cccttaatgg ggcagcctaa ctgttccgta 60120 ttgcggattg taaacaagaa aaattagcaa aatcaatttt tactaatgtc tctcaattca 60180 atataaatat tatatcaaga ttttgagcat cctgcaagta aaatttttcc gaaggtgcta 60240 tcagtgtctt tgagcacaga gaaaaccgag ccaaaatgac tcggtaatca ggattgggct 60300 aatgaaaatc aatcatcatt taggcctggg acatagatcc caatgcgatt taattgacct 60360 ttagcttctt cgagaatatc ttcggtgtac tcccaaatat catcttctac ttgcaaaaga 60420 taaggggatt caccagtttc ggggtcacgt gtaatggcta agccacgtgg atggaatata 60480 gagaagttga gggcaacaat caagcccatg ctggacaatt cattccagtc agcttcccaa 60540 ccatcatctt catcttcatc cacaaagact cctacactag ggtctgttac agaagtaaac 60600 ggaaatgtta cactaacctt gtcaaccccc aaccaacgtg caaggagccc ctcagcacgc 60660 tctaccaaac taggatctat ctgacgccaa aatacttgtt ccattctgcc tccttaagca 60720 gttaaacctg ggatcatata cccgaaacga cgaagtttct gaatcccctc atttacttcc 60780 ccgggcgagt atgaccagcc ctcacgttca tttacctgga aagcaggagc ttcaccagtg 60840 cgataattta caataagacg gtgatttgca ggcataaaca gcttatggtt caaggcaaaa 60900 accagaccca tttcatcaag ttctttccag tctacaatct cttctactcc ctcgcctaaa 60960 tccacactaa ctccaataca cggagcttcc tctagcgggc ctttaaaaga caggaagata 61020 tcgttattct cacaccaaat cttaaggaga ttgatggttt cattatacat accattaggg 61080 agagagcgcc agaagttgag aagcatagga tttacctctt tcatgaaaat attattgaac 61140 atgcttatac cttaagtttc tgaagagcct tacacatttc aagatctgga ctaaactcca 61200 gcatatagct gatagcgttt aaatcttgaa ggtttattcc agtcctgccg tttacaataa 61260 cagtaggaaa ttgatacatc atagcaccag catcatctag gacacaccaa tgcttgagct 61320 tatatttttc gacaaaacgt aaaacagaat tacctcgaga taaaccacct ccagtaaaat 61380 ctgttgtacc aagaaaacga tcgatcaaac cgagtccggt cataatctga acgttctcca 61440 tttcatttcg tacagaaaac catgaagata ccccaacaat cattattggg gatggctcaa 61500 gaaagttctg aaaagcctgg agtaatggct tgtaaaccca atcaccccca aagaatagct 61560 tctcactatc tggagcatga tggtgagaga tgctcgaatt gagcacccca tcaatatcca 61620 ggaaaatcac aggagtatta cttatttcca ccagtagcat cccacgtcca gactttaact 61680 tcatcagtaa aaggaactgg aaccttcaat tcacctttac cccacataac agataggaac 61740 agagaataaa tggtaatcat accgattagg ttgacaaacc atactagacc tttccagata 61800 cccttaaaaa tcttcttcac catattctcc ctccttaata aggaactctt tccattctgc 61860 tgtatcctca tagataacgc agaaatcatc actcttccat gagaagtcat cagtagcaaa 61920 ttcgaaacgg tagcaccaac taccgtctgg ccatactaaa agatcacaca tgcttttctg 61980 ggacataaat atttccctcc ataaaagctt caacttgatc aataaagtgt ttattaccaa 62040 aaacttcggc agctccagca cgccatacag taacaccacg tttctcatac tcaagctgtt 62100 cgtcataacg aacttgtggg ttaacaacca tcatcttagt tccacgacat acagcaggaa 62160 atagctccca gttgaagtcg attacttggt tgctacaacc tactacaata accatatcat 62220 tgctagtaag tgtatcaaac aggttaatct gacccatata ccacggagcc gtctcaccaa 62280 agaatgttac gtttggtttt acccattcgt aatcctcagg ggcaatcgca gtatacccaa 62340 catcttcgat gatttggttg cgaccctgac gatagatcac ctctttcaag tacccgtgag 62400 cgtgaatgat ttcatcacga gggataccag cacgttccag aaggtcatca acgttagtag 62460 tgaagttctt cacgctatcc tcaccataac gctggaacca ttctgcaatt cgcagatggg 62520 caatgttagg ctcaacagtc tgtaattcct gacgacgcat gttgtagaac tcatgagtct 62580 tgagatacag atcgttacca tcttcatcaa tgccagggag acgagcactg aagtagttag 62640 gatcatcttc accacagaag cgacagaagc cagcttcaaa gttaggcaag ctacatactt 62700 cctctagatc gtactcatcc cacaaggatt tcccactagc agtatcagta cggaaagcac 62760 gaacaccact atctacactc aaacctgcac cactaacaac gattagtcta cgcattatac 62820 gattccttgc ttgaatcctt cgatgatatt ttggagcaga attgcttctt ctgggcgaac 62880 cttctcttgc ttaataagca tatccaaacc agcaatacag gtagctactc cagcattata 62940 cccagcattc cactgctcca taagcatatt ttctaaatta ctttttagat tctgattcaa 63000 gaagtccata aagtttttcc tccaattcca taatacgatt ttcataagaa gcacgcagct 63060 tctcgatgga caaagcacga actgtaactt catctgaatt gatcgctagc tgagctagat 63120 cttctgtttc catacgtccg ttgtaaatta agcgatattt aagatgtttt acgtttttca 63180 ttcttattcc ttagttctgt actgtaaatt cattcttatc acagtgagca taataatacc 63240 catacatacg ataaattttc acagccttag tagacccctt cgattcttca taatcaatcc 63300 agagccactt gtctttacct acataatgta cccgaaactt cttaactttc ttatcattcc 63360 atgttgcaac aacacgacga taatcaaatc cgcccaacca atctagagcc ttacgttctt 63420 cactaggctc tccaagatgg gaaacatcta ctttcggaag caattcgctc atagctttct 63480 cctctcaaat ttataaatca attatacata agttttagct cgaaagcaaa taaaataaaa 63540 gccaggtcga ctagcaacct ggctgattca ttatttagcg cgaatgtctt gatgtttcac 63600 tcgacccata acctcatgtt gtttacctgg gttaaacgga cgagcacctg gatttccaag 63660 atatccacaa actcgacgag ttacctcaag agtctcagga tcatgattgc cacaaattgg 63720 gcatttgaaa ccatcctcag aagctatagt ttcacccaag aacccacatt ccccacaaga 63780 gtctactgga gtattaatac cgaagtaatg aactttactt gcagcatagt taataaccca 63840 ttccagtgca tcagggaatc gtttcatatc cggtaattcc acataggaaa tgcacccacc 63900 agaagcaata ggagtaaagt tagactcata atcaaacttc acatttggag caaccttacg 63960 ttctacatca aggtgatggg aattagtgta atatccttta gccagaatat cttcatgctc 64020 tgggaaatac tcacggtcta ggcgacagaa gcggtcacat agagattcag atggtgtagc 64080 atacagacta taccctaatt gagtctcttc cttcttctga tcagcacgat cacgcatgta 64140 ttgtagcaca cgttgaacga acttgataca agactcagac attgtatcta catctttacc 64200 aaacatgagc tgcaacattt cgtggcaacc aatatatcct agagatacag aagcacgctt 64260 gtagaaatga tcaaatacat attcatctgg ccccagacgt aatccaaagg ctcctgacat 64320 atagaggatg ggcgcagctt tagcttgaat gtattttaag cgatcaaccc tccactccaa 64380 tgcttttaac gcggtatcca cacgcagctc aagcagatcg aagaatagat caatatttcc 64440 ttccgcttcg atagcgattc gaggaaggtt tacggaaaca acgcctaagt tgttacgacc 64500 agaagtttca ccatcttccg cagcagccag gaacgaacga cagcccatag agactttgta 64560 gtcccccgta acttctacaa ccttatcata gctgatatag tccggataca tacgttcagc 64620 agtacacttc atagctaact tcttgatatc atagttaaca tcgccaggtt tcatgtttac 64680 gccgtcacgt agaacaaata ccagtttagg gaagatagca gtacgtttat tgatacccaa 64740 accatccata cgcacttcta agattgcttt ctgtacaaga cgtgcttccc aagaagttcc 64800 taaaccgaaa ccaaaggtaa taaatggact ctgaccatta gagttgaaca tcgtattgat 64860 ttcgtactct agaccctggc aggcatcgta aacttctttc tcagtcattt ccgtagccat 64920 tacagatgct ttggcttcat ccttagtcca acgttttgca aacgcatgat tcttatcata 64980 agacttacga acataaggag ccaatacttc atctaatcgg tctacagaag taccaccata 65040 ttggtgtgag ctaacctgag tgataatctg agctgtaata gcagccgcag tagtgataga 65100 gtttggcgta ctgatttcag cattaccaac cttcatacca ttcttcaaca tatcttccaa 65160 agcaaccagg caacagttag tcatacctaa cgcagcgtaa tcagcatcat ggaagtgaat 65220 atcaccaatc atgtgagcat ttcgaatctg gatcggcatt tcttccagaa tcagatattt 65280 actcaattcc cctgcaagca tatccctttg tgtagggaaa cgttctgagg gcttattggc 65340 attattaaac agcagctctt catctgctga accttcgata atttctttgc agtttcgaat 65400 caaggactgc attcctttat actttagttc ttcattgcga ttcattatac cctccttctc 65460 taactaagac tactattata actcattcga aggcggatgt caacaatatt ttactataac 65520 attctgtaaa ttgatctgag tcaaaaaatt ttatttgcca acctctacta aaaatagtat 65580 aatcacgcac ccgtgtacgc gatataaaat gattatctag taccatacat ctcccaataa 65640 aagcaagcaa attttttatt taaatgcaat atcataggct tatttaaaat tgtagttgct 65700 ttctcccgca tatattggta taatatattt gtaagttgat aaacaagatc tttttgttga 65760 tcctctgtta gcgaagtaca ctataggagt gttatttatg ggaaaagcac gtcaaaaaag 65820 agagaaccgc aatggttcaa gaaagcgtgg caacaagtac gagagtaacg taattcaagc 65880 agatttctct ggtgattacg ccaatccagt tgctaaatcc ctagttggta aaaaccgtga 65940 gcaaaaatca tatatcaata tgatcaagaa caacacagtg actgtgggta ttggtgagcc 66000 aggtactggt aaaaccttta ttccatccgt tcttgcagct caggaactcg tagacattca 66060 ctcagatatt gagcaagtgt tcctcgtgcg tccaaacgaa cctctaggta aatctcttgg 66120 tatgctccca ggtgatctag cagaaaagct agagccttgg ttggaaccaa tagccgatgg 66180 catgaaatgg gcaatcggtg atcacgccta caaaggatat gtcgaacgtc agaaaattaa 66240 attcctggct atcgaacatg cccgtggccg aactttcaac aactcctatg taattgtcga 66300 tgaagctcag aatatctccg ttgaagcaat gatctgtctc ttgactcgtg tcggacaaga 66360 ttgtcgtcta attatctgtg gagatatagc tcagaaagac atcaagggtg attcaggtct 66420 agcacttctt atggaagtct atgagaagta cgaaaactct cctttctcta tgatcgaact 66480 gatcgacaac gttcgctcac cagagtctaa agcattctac gatatcttta aggatatggg 66540 gaaggtgtaa tatgggaaac gtcgtacatc tgcaccgcaa aactaagatc caccgtactt 66600 cattgagtgc tgctaatatg attacacgta aggaaggaga agaaagtcct aagactaccc 66660 tagcgtggaa aattgtagcc tctaatccaa atcggccttt caactataat gaactgagta 66720 cttctataga cattcttatg gctaaggaaa tacgacagaa actaactgat atgaccgata 66780 aacttaaatg gtaagacaga cgagcctggg tatgaaaata tccgggctct ttttatctgt 66840 aaaattgtac ttgcaaaatg acccaaaaca aagtagaata tttcctaaat cctgataact 66900 acaaaaagga aagccaaatg agccatcgca tcgaaaaagt aattaaacgt gacggtactg 66960 ttcaggactt tgctcctgaa aaactcaatg attgggctgc atgggcatgt aaagatactg 67020 acgtaagttg gtctgccgta gttatggctg ccctgaaaaa tctacccaaa ggtgttgtag 67080 actccgatac actccaagat gtgttaatta aagccgctga aggtcttatc aaagacaacc 67140 cagcatatga tactgtagca aaggaattac gtcttgcgca gatgcgtaaa cgcctatatg 67200 attcctttga acctccttct ctgcgtttct tccacgatca tatggttcgt gcaggcgctt 67260 gggaagacat gagtgcatgg attagcgatg atcagtttga cgctctgaac caagttattg 67320 atcatgatcg tgatcgttta ttcaccagtg gtggactgaa acagttcttc gacaagtatt 67380 ctcgtcgtaa tattgcaact ggtgaaattt atgaaacccc gcagtttgcc tatatgggta 67440 tggcaatggc gatgttatct caacctaact ggacaattct cgatgcaatc gacctctaca 67500 acgcaatgtc actccataaa atcaacgtgc cgactccacc tctcgtgggt cttaggagca 67560 gcgaccgcgg ttttgcttct tgttgtcttg ttgatagtac tgataccctc gattcaatcg 67620 acacagccga gcatatcgtc ttcaaaatgg tcgcagccag agcgggaatc gggtatcatc 67680 ttgaaagccg atcaattgct gatccggtgc gaaatggggc attcccgcat tccggaaaac 67740 tgccatatta tcgacacatt gaccgttcag taaaagctaa cactcagcaa acccgtggcg 67800 gttctgctac tgtgtcttat ccatacttcg atcctgaaat cattcaattg atgcaggtta 67860 aacaacaacg tgctacagac gagaataaaa tcgataaaat ggattactct ctgagcttta 67920 acaaccttct gttaaaacgt tacctgaaaa acgaagatat tacgctaatg tcctacttct 67980 acgcaccaga agttcatgaa gcgttttatg atgaagatga ggctaagttt gaagaaatct 68040 acgtggcagc ggagaaacgt gtggcatctc ttacaaagat cgaccacgaa ggaaaaacaa 68100 ttccagcagc tcctagaatc tctgcaaaag aaatcctaga tacttggtta cgtatccgta 68160 tggaaacagg gcgtatgtat gctcatcaca ttggtgaatc taaccgtcat ggtaacttcc 68220 ttgacccgat tcgtatgacc aacctttgtg ttgaaattac tcagccaacc cgtccattcc 68280 atcacattac ggaactgtat aagacccaag atcagttaga cgatatgcgt ccagaggaca 68340 ttggtgaagt atctctgtgt aacttaggtg gtgttgtact aggtcgcatg gaatctctgg 68400 ctgaatggga gaaaacttgc tacatccttc tgaaattcgt tgatacaatc atcgagattc 68460 aggattatcc attcccaacc atggaataca cggctaagaa acgtcgtaac gttggtattg 68520 gtctgatgaa tgcagcaggt gcaatggcag cagaaggttt ggcttatgaa ggtgttgaag 68580 cccgtaactg gattcaccgt gaagctgaga agctgtccta cttcctgcac aaagcctctg 68640 tacgtctggc taaagaacaa ggtgcatgtg actggtttaa tcgtactaag ccatccaaag 68700 ggactctggt aatcgacacg tacaagaaaa ctgttgatga actggtaact gttgatctag 68760 aaatggattg ggaatctctg cgtgcagata tcctgaaata tggtatgcgt aactccgttc 68820 tgactgctca gatgccgggt gaaagttcct ctgtactgct aggtgttact aactctattg 68880 aaccgcctcg taagattgta tctatcaagg gtagtgcagt gaataaagta attgcaattg 68940 ctccaggtgc aactgattgg gatacattaa tgagctataa actggcttat gatgtagatc 69000 gtatcgagtg gatcaaatgg gtagcaacca tgcagaaatt cttcagtcag tctataagta 69060 cgaatatgta ctatgactac accaagtttg agaatgaaat tattcctggc ccagtagtag 69120 ttcgtgactt catgactgct gttaagtatg gttggaaaac ttggtactac gctaacttca 69180 acactgaaaa tggtggtgga gctggtgaag aagcagcagg ttgtgcatcc ggtggttgta 69240 acctataata aaaacaagga gatcttcggg tctccttttt ctttgaaatt ttacttgcta 69300 atcgctacca gatttgctat aatatctcta tattaaggag atatactgtg aaacctcaac 69360 taacaaaaga atttctagag aaatatctag aactcagaga agatactctc tattggaagc 69420 caagagagac taataaaggt ggatttaata ctagatttgc tggaaaacct gtaggagccg 69480 tcaatggaaa tggctaccta tgcttccgac ttaacgtgga aggttataga tgggaattaa 69540 gaaatcatgt agtaatcttc tgtctctatt atggatatta cccaactaag acagtagacc 69600 ataaagatac aaataaactt aataaccatc ctttaaatct acgagaatca accgatgctg 69660 gtaatgccca aaatagggga gccggtaaaa acaataccca tggacataaa aacctagagc 69720 ctcttagttc aggaaactgg agggttagga taacacacaa cggtactgtt tacagatctt 69780 ccgcaatgcc tctagaaaaa gctctagaac atagagatat taaacttaaa gaatttcatg 69840 gcgaatacgc taataatgga gaataattaa tgacaacctt actaaatctg aactggaatc 69900 ctgattctga agagctattt ctcggagatg ccctgggagt agcagactat gttcgtgttg 69960 ctcatccaga gctagaacga ctcgcactat tacagaggag ccagttttgg actgaaactg 70020 aaatcagtct agaaagtgac aaaaagcaat ggcctaatct tccaagagaa atacaagaaa 70080 tcactctgct gaacttagct tggcaggttc aaacagattc tttcattagt cgtgctcctg 70140 aggcagctat tatgccactt gtaagccgtc ctgaattgga aggtatgctc aagcagtgga 70200 gctacttcga agatctacat agccgtgcgt atagtaacat tattcgtaac gttctgactg 70260 acccagcaga atttattgat tctgtaacta aaaaccaaga agcatttgca cgtatcgcag 70320 attctgttga actgtttgat gaattgtatc tgttaggtca gtatttcctt gcagtacgtg 70380 atcatcgtgg tgataatact tatccagaaa ctgagttccc tgaagttaaa cgtgaaactc 70440 aggctaagct gttagatgct tactttgcaa tctacggtct ggaagcaatg cagttctacg 70500 catcttttgc atgtaccttt gctctagcag agaacgatat tctgcaaggt attgctaaga 70560 acctgcaatt aatcgctaaa gatgaagctc tgcatactca aatgtctaaa gctatcatcc 70620 agattatgtt ccagcagttt gataaagatc tggtagatga agcattagct aaagcaccag 70680 cacaactcct gaaaactctg aaaactgaga ttgaatgggg tcactttatc tttaaaggcc 70740 gtagtctgat tggtttgaat gcagaactgc ttgaagaata cctttacttt gtaggacgta 70800 acgcatttat gcacattggt gtagaatggc caagccactt acctgtaatt actaagaacc 70860 ctattccgtg gattatgaac tggctggata ctacttcctt acagcctgcc ccacaagaaa 70920 tccagattgg tgcagcatat cgtgtgggtc aggtaactga aacttctgct gataccctga 70980 aagatctggg taacgaattt gagggcatgt tctaatgata gcagcaatct atgcaactgg 71040 gccgaatggg gagttcggcc tcaatggtaa gctcccctgg ggttctttca aagaagaact 71100 agatatcttc tatactactt tggattctct agcacctgat aatattattg taggtgcagg 71160 aacttggtta gccttccctg agactctgca aaaaagaata ctaggagatt ctgacctatt 71220 tatttatgca aatagaccac tacctaactc tgtaggatca gccacacgca taagtaacat 71280 tggatacagt ctacctgatt tcctcaagga agaagtaact gtagtattag ggggagcaac 71340 tctgcttctt gaaatgtatg tcaaaaatca tctagatatg gcgtatatct ctactattac 71400 ttcagctaca ccattatctg ctgatgtaca tctgcatcat cacattactt taactaattc 71460 aatggcaact agaatggatt atgtagcagg acagaatgca gataaaacgc tttcattctt 71520 acaagaattg gtgatattta aatgaagcaa tatattgaaa ctggaaaacg tattcttgaa 71580 ggggtgtat gggtaacgaa tcctagaaca ggggttcgtt gtctaaccat tattgatgca 71640 gactttacct ttaacgtagg agtaggagaa ctgccgctag tatctactcg taaagcattt 71700 gctaaacaag caataatgga aatgatcgga tatctccgtg ggtacgactc agcagcacaa 71760 ttccgcgcta ttggtgttaa tacatggaat gctaatgcaa atgaaaatat tgcctggcta 71820 ggtaatccat tccgtaaagg cgaggatgat atgggacgag tctatggtgt tcaaggacgt 71880 cattggacta attctgatgg tatggtattt gatcagttag ccaaagtcta tcataatttg 71940 aaaagaggga ttgacgatcg tggagaaatt ttaacttttt ggaaccctgg ggaatttcgt 72000 aaaggatgcc tacgtccgtg tatgcatacc catcaattct ctattttagg tgatacacta 72060 tatctaaact ctttccagag gtctaatgat ttcttgctgg gccaagtatt taatatggtg 72120 caatgctatg cacttctagc tattatggca cagattacag gtcttaagcc aggtattgct 72180 cgtcaaaaga ttatcaatct tcatatctat gaagatcagt ataatgttct gatggaacac 72240 aagcaattcg atcgtgaacc ttttgcatct cctactttag aaatcaatcc aaatattagg 72300 actttggatg atgtattaac gtgggtaagt aaggatgatt ttactgttcg tggctatgaa 72360 caccatcctg ctattgcata tccattcaca gtctaattca aatagccctt cggggctatt 72420 atatttaaat ttcatttgct tactgagtga aattcaaata taatatattt tataaattgg 72480 agacacttat gggactcttt aaaaagaaag tttatttaac acaaagtcaa gaacgcaggt 72540 tcttagctct gtctcagtat taccatttac ctttcgatca tttcatagaa cgggcgttag 72600 aacaattcct agaaaaggaa ttaagggcaa ttagacctga accgccaaga gcaccaaatg 72660 atgcacagag aggtcatcca cgtatcccac ctatgccgcc agttccacca gcgactaggg 72720 tcattataga aggtgaaaaa taatgtctat tcttttatct gctttagaag caaaacaaaa 72780 tactattgct agcattaaaa aggaagtaat tgacctagaa tcacttattg ccgtagagat 72840 tgacaaagct attaaagata aagaatttaa ctgtcacatc cagggattaa ctgaatactc 72900 agaaaacgct agaaatagcg ttatgcaaaa acttagcaaa ctaggttatg ttgtggaagc 72960 taagtatgaa cagcgtgaag gtttttggct tgaaattgat tggagcaaat aatgtcggtt 73020 ttccacatat atacagatgg tgcatgtaaa ggcaatcctg ggttaggtgc atggggattc 73080 gttgtctata atgataatga tgatcgtata ggatctaaat ccggctttag ccctaaaact 73140 actaacaacg aaatggaact cactgcaatt gtagaagttc ttcgttggtc tactaagaaa 73200 gataatcgtc cagtaattat ctatactgat tctgcttact gtaaaaatgg tatggaaagc 73260 tggatgtggt cttggcagaa gaaaggctgg aagaagggag atggtgaaac tcctcttaat 73320 ctagaactct ggcaagaagc atttaaacta actcaacagt atattaattt ccatgatacc 73380 aacccaacct ttgttaaggt taaaggtcac tcaggtattc gtggataatga agaagttgat 73440 gctctatgca atactgtaat tactgagcaa gaacttgctg agatgtaata aataatttaa 73500 aaattcactt gcttaaagcc ctcgaattta gtataatatt cgtattgaaa gtgaggaggg 73560 ctttatgcga atttacaact caaattcatt aggatttaaa cctttgagaa aacgcgcaca 73620 atctccgcgt cagatcagaa aggcaaatat tggtgagcca gaaactaggc ttccaccacc 73680 tccggagcaa cgtctagttt acctagatga agaattagcc gaaagggaac gcaaagctca 73740 agaagaaatt gagcgcaaga aactatgtac tgctcctgca tataataagg gtggttatca 73800 atacgtttcc agtgaggaac aagctaagat gatcggcaga taatttttaa aaaattcatt 73860 tgctaaacgc ttcaaaattt cgtataatat atctcataaa ttgattaaca aaaaggaaaa 73920 caaataatgg ctaagcagaa aaacgcaaaa actcaggcag ctccggcagt taaaactttc 73980 gctcagaccg aagccaatcg taaggctcgt ctggaacgtc atctgcgtaa gcatcctgaa 74040 gatgttcagg ctgcacaagc tcttggtcgt ccggctccgg ttcgccagaa gccgaaggct 74100 aagaactcca ctcgcgcaac tgctaagcag gttgtgttca tcaagggtga aggtcataag 74160 tctgtaccgg taactctgag ctttaacggc ggtgctgaga tgttcgcccg caacggtatg 74220 gctctgaaag actacgaaaa agcagtcaat cagaagcgta agccaatggc tgacgtaatg 74280 cgtgaatccc gtggacagtt tggctccgtt aaaccgaata ttttcggtgt agagtatagc 74340 cgtgacaacg ttcgcgctct gtgctacggt atcggtatta agtttactgg tgcttctgat 74400 cgtaaatccg ctaaacctac tcgtaagcgt aaagcgaagt aatgattgat ggggcgaaag 74460 ccccatatac taactctttc tctgactaat taaggatatt tttatgttct ctaagaaacc 74520 acgttccgtt actgaaatcg ttgcttcctt taccactatt actgatgaac ttcaagctcg 74580 tattgaagct gaccagaaaa ctgctgctga tattcagaaa cagcaggaag aactcgctct 74640 gaaactggct gaaaccaata agagtgaaaa atctgctcaa actattaagg aaaatatcct 74700 taaactgctg ggcaagtaat ttaaatcagg gggcgaaagc ccctcttatt tggagaaact 74760 atgcgtaact tcgttgcaaa gaatgatttt aaccgtgcta gcactcacaa gtctgctaga 74820 gactacactc gtctttctaa acatgagctt ctagaagaaa tcatggaaga aaactgggat 74880 gttagtgaaa atggaacaga cctgtttgaa gaagaggaac ttcctctgaa agaccccaaa 74940 cagtgggagt ctattaaaga gacagctctt taatagtttt ctacaacaat aaaggacata 75000 attatgaaat tcaagccctt tggtatgctg gacattgaat ctctaggtac tccaggaaat 75060 tgcggtacta ctcatattgc aatgccttct ttcgcttttg tagcgatgca cggaattgat 75120 aaagatcctg atctggtatt cgttacgcta gacgttcaag atcagcttaa tgcgggtgct 75180 aaggtaactg cctctactat tgcattctgg atggatcaag ctaaaaacag tccatctgcg 75240 ctccatatta tggaggctct aaatggtcgt aatcctaaac tgattgcctt ccgtgatggt 75300 aagcatcact gcactaatga cgtaggctct aattatgctg ccttctctat ggctcagaac 75360 attatggaat tgtctctagg agataatgct ctgtactacg gcaatggccc agagtttgat 75420 atgacaatct actctgcaaa tacgttccat gccggaaccc atgaagaaat agttccgtgg 75480 aaattctgga acttaggtaa cgttcgatct ttccgtaatc agtggatgca agctggttat 75540 agctataaag atctagaagc cgaaggtgct agctgggcaa aagctaaaat ggaaaagatg 75600 gacaccattc gttatggaat ctatcctgtg aagcatgatc cagcattcga tgctttagtt 75660 gaaagttatt gtgtagccgt catgatcgaa aaaatcaaaa tttgatttgc tttcagctcc 75720 aaaattctgt ataatatatt cataaatttg agagaacaac aaaaggaaaa caattatgcc 75780 agtatctaaa aacgtccgta aaaatggtaa agtagcaaaa cgtaacctgg gtctgcgtcg 75840 tatggctgaa cgtcagtccg gcatccagaa aatctttaat gtactgaatc gcgccaatcc 75900 gaatattgat aacagttctg atactgttct gcacactatg ctggcaatcg gtctgtaata 75960 ggccgttttt aaacaaattc aaatctcaaa cttcatataa ggaagcaaat aatgactact 76020 cgtatctcta aaactcgtgc tctggcaact atcaagtctc tggaagctaa aatccgtaaa 76080 gctactgaac aacagctttt gatcgatgtt ggtgaaggta aggacaagaa ccaagttgtc 76140 gttggcgctg caatcgaagt agatgctctg tctacccgta ttaagactga tttccagtct 76200 ctgctggata tgatgtccca gcgtgatcgt ctgaaagctg cgctgatcaa gagcaatgct 76260 gaaactgttg tagagatcgg ttcgcgctct atgactgttg cggaagcaat cgaagctaag 76320 cgttctatgg aactcaaagc tcagttgctg gctaacatgc gtaagcagtt ccatgctgct 76380 actgttaagt ttaacactca gaaagctcag tttgatgcta agtatgagcg tttgcaggat 76440 accatggcta cccgcgacaa gaagacttcc gaggatgaag ttaagatgca gcttaacctg 76500 ctggaactca agaacactcc gttcctgatc gatccgctgg ggctggaaaa gctgatcaag 76560 caacacgacg aagagtacca ggactttgca actaacgttg actttgttct atctgagtcc 76620 aacgcctcta cttttatcga agtagagtaa taatttaaaa gactagtaaa tcggtagacg 76680 aaagcccaag catcttacct cgcagcagcg gttgaaatac gaggttaaat aagaccgctg 76740 accatactat aaagtataat ctattggaga ttatgtttat tacaccagcc tcttaagctg 76800 aactgtaaaa acggtcgttg gtagtaccaa cacaacttca aagatcaacg ctcaagtatg 76860 aaagctgaaa agttaaaagc actcaaactt ctaaccttct aaagctaaaa gatgcaaagt 76920 attcgttgat gattcaaacc ctagatcaaa ggtacatggc tcataagata tggcctgtgg 76980 cgcctctagg ctgtttatcg gtttactagt ctctcaattt aaaaatgtat ttgctaatta 77040 gtttagtttt ctgtataata tatttttaaa ttgatgagac gaggctatta tgatttttca 77100 tattctgatt gatgatgttc gtgacctaca tgggatggat attatcattc gaacccccga 77160 agccgctatt gagtttctga atacaaccga taccaccgga catttcgttt atatggataa 77220 cgatctaact gtagatggta tggaaggtta tcaaattctt cgtttactcc tagaatttgg 77280 acagagaccc aagaaagtgg tactagtaac ctcgaaccct gtagctaagc agaacatgcg 77340 caatgatttg cttgacgaag ggtatcaaga aagccctaat cgtattgagt acgattggcc 77400 ggagtaaata aaatgaaggc agctttgctt ttaatagctg ccctaaccta tagcttcaat 77460 agtcatgcga cctacgactc aaagcagatt gattgcattg caaaaaatat ctacttcgaa 77520 gcacgaggtg agggcactaa aggaatgatc gcagttgcac aggttactaa aaaccgtgtt 77580 aactctggta cttttcctga ttcttactgt aaagtagtat atcagtctaa ccagttctct 77640 tgggtggcca agagaccaaa agttgataaa accgatgagg cttggcaagt tgctaagaat 77700 ttagctagag tgatatacta cgttgattta ccacaagatc caacacatgg agcgttatac 77760 ttccatagtg gtaaagataa accgtactgg acaaagaagt ttaagaaaac aacgaagatt 77820 caaggccata cgttttacaa gcctgtggtt aagaaaactt aaaaaattcg attgcttaaa 77880 gccttaaatt tctgtataat actttcataa attaatgaga ggaagcgaaa tatgaaatca 77940 gttgtaatta ttggtgttaa tactcgtggt atccgtacta cgaaaacagt taaaactact 78000 aacgttgctg aaattactac aaaccctaag aagtttgatt tcgccaaagt ctcggcagtt 78060 atgacagaac aatcttttaa ccaagcactg ggaattaaat aatggaagag aatattctag 78120 atgttttgcg tagggctttg caggaatcta aggttgatgg ttctgataaa gaagtagcta 78180 aaagctacca gaccataatc ggagatcttc agcgtgttga taaagacttc attacttctg 78240 aacagtttgt atcttatcta aaagctcaac tgaaaagcat taatcagacg aaagctaaac 78300 ttcacggtca agaccttgat agttatagct tgcagtctgc tcagtatgaa tatatactga 78360 acaaatggtt acaacagtat ctgccgcctc agctttctga ttctgagatt cgcaaatact 78420 ttgcagaatt agttaagttg aaccctggga ttacaaaagg tatgctgatg aaagcgatta 78480 aggaagagtt tcctggtcgt tacgacggtg gcattgctgc tcaggttgct ggcgaattta 78540 attaaaaatt gatttgctaa acgcctcaaa attctgtata atagattcat aaattagaga 78600 acagcgagcc gattacgctg agtgagttta gtgattccta ctaggatagc tattccactg 78660 aatagagtcg gtgagtttag aatgatcacc tgttagagtt ccgagtccag tccggtatat 78720 ccggccaagt gactagagta acgaatatag gttcgagact tcttgtaaaa ctcgggagct 78780 gtcccgaaac ctcgtaaact cttctctaat ttatacaaaa cgatggttct caacatgagc 78840 taggcacagt gtgaaatacg gtgcgtgtcc caggggtaga agactgaagg ttgggcttga 78900 gaaacccatg taactttcaa cgctgacccg tctaggatgc aagtcaagac gtatgtatac 78960 ccaatgcgg cgattgtcca tcgaatagtg gggtgtcgaa ccgacttatg tgataacact 79020 gccagatgct cacaggctga cattgagctt cgctgctcct tctggaaaga atcggataac 79080 tcgggcttat accggataag cattcgctgc tggagtacat actatgacgg ttcaggaggt 79140 acgccgcctg atcagtatta ggttagaatc gaaacagcta aaccacgagg aaacctgacg 79200 gcctcaacca aactcggaac tcgggatcac tgcctagtgt gtgccccgtt taacatagag 79260 actaggcatc cagcttcgca tcgaaaatcg gaggtgcgat tctaacagac cgcgtgaata 79320 tgagagtggg aaatgctggc atgagagatg tcactaatgg tgcccaatag tataggtaat 79380 tcgcgataat tcctgttgag tggcagcagt aggctgggag tcatgaccta agcctaaccg 79440 agttcttcac atacggagaa cataaccaca aagtcaccct gacttaaact ggaggatatt 79500 atctgacgga agggctgcag agcttaatct gagggggctg accctaccta ccgtcgggta 79560 tctttaagag cttttataag agttcttaaa aatacctaaa cggggtgtag tctaagggat 79620 aggcaggagt cttctaaatt cctttatgca ggttcgaatc ctgtcacctc ggccaatttt 79680 attaactgct aggaaaataa agtgaaaaca cgatatatag taaaagtagt aggtgaacat 79740 tcacaagtaa ataccaccga tgttacagcg ctagcatttg aagtagtctc cggtatagtc 79800 atatttcata acggagatat caaagctcct gaatttgctg tggatgctag cctgtttatt 79860 tctgcaacta aagtaactgt agtagctaac tcttctaaat aataaggaaa tatatgttta 79920 ctaaaaacac taaagaactg acttccgatc aaaaagtaat tcgtactatt aaacgttggg 79980 gtgttggtgc tgtagttggt cttggcgctc ttattctggc tctcaattcc tatacagttg 80040 ttcaggacgg tacggttaaa actcagactt tcctgggtaa agttagtcct aatcctgtat 80100 tgccggggtt ccatattgtc aacccattcg cctcttttga tacttttagt acgaaagata 80160 tttctatgaa actggataaa cttcaggttc ctagccagga taagtttaag tctacagttg 80220 acattaccgt tatgttgcag tttgatggtg ctaaagctcc tatgaaccgt gttaacgcag 80280 gtactcagga tcaagctctg aacaagtacg ttgaagagaa gatgctgtct acgattcgtg 80340 agtttggtaa gtctgtacca aaagcacaag atctgttcga cgctaagatt caggctcaac 80400 tgcaaaccgc tattcagcaa gaagtagaag agtacgcgcg tccttatggt tacaccgtta 80460 agcaagtatt ccttcaggat attactctgc caccggtaat catggaacag gtacaaaaca 80520 caaaagttcg tgaagaacaa gttaacgctg caaaagctga actggctcgt gttgaacaag 80580 aagcacagca gaaagttaag caagctgagg cagatcgttc cgctcgtgaa aacatggcag 80640 tagctaacga acgtgacgca gatgctaaac tgtacgcagc taagaaagaa gctgaagcta 80700 acgccgctct gcaacgtacc attactcctg aaatgattcg ttggaagcag ctggaagttg 80760 aaatgcttcg tgctgaaaaa taccaaggtg gtgtaccaca gactgttgtc ggtactggct 80820 acgatggtca aatgatcatg gatatgcgta ataagtaata gttacacgta atcttcgaaa 80880 caagcccgcg tttcccccga gagcgcgggc ttttctgtat ttaaggcaaa agaaaagccc 80940 ataagttatt caactaaggt ctttagtatt taattcttta gaatcatata ctattattgc 81000 agcaacaacg tgctaccctt aagtgccctg cgattttcca ctaaaaagtt taaaaattca 81060 attgcttgaa tgctaaattc tctgtataat atatttataa atttgagaga ggaatcaaat 81120 atgattaaga ctggtctagt aattggtaag ttcgcaccat taacgcgtgg tcatattaac 81180 ttaattaata cagcagcaac tatgtgtgaa cgagttattg ttgtagttag tcatgataat 81240 cgttggctga ataagcagaa cacacgtgat caggaagtat tacagttaaa gaatcgactt 81300 cgttggttag aacaaactta cgcggatatt gagcacattt cggtagagta cattattgaa 81360 gatgatattc ctgaatatcc gaatggttgg ccggaatatg ctagaatcct taatgaaaat 81420 tacggacatt tgcctgagat caccattttc tctagcgaac tagactatga tgcagaatat 81480 aagaaatatc tgccgaagtt tggtcattat attgtagatt ctgatcggac tcgtgttcca 81540 atctcagcta caatgattcg taatgatctt atgaagtatt gggagtttct tcctagcgta 81600 gttcgtaaag attacgtgcg taaggttgta atcattggga ctgaaagctc aggaaagact 81660 actttaacaa aataccttgc gaagctatac aacacttcgt gggtagaaga atatggccga 81720 acgtattgtg aagttgatct agttggtaac gaaaatctgc ttaccagcga agactatagt 81780 aaaattgcgt tcagacacaa agagctagag tttgaagcag aacgtactgc aaataagatc 81840 atgittgtgg atacaaatgc ttttattact gagttctacc acagacttta tgaacatagt 81900 aagaaccctg tggttacagc aattgcaaaa gaagaacatt acgatttagt aattgttcta 81960 gcgcctactg tcaagtgggt agatgatggt ttacgtatta actccgatcg ttcaattact 82020 tctgaactgt tcgagcagat gaagaaagaa tttccaaatc agttccctga agggcgtact 82080 gtttacatcg actctccgga ctacaaagtt cgtatggacg aagcagtaaa cgcagtagac 82140 tggctgattc atcactttaa cgttggagag ttttaatatg actaactatc tcgtaaatga 82200 ctttaaaaac tggaaacctc tggaatatct gtggctgttc atagcggttg tcttcattag 82260 tttagcaaac tataatggta cagctctaga ttgggctact gcaataacca atgtggtttg 82320 tgttattctg gttgcaaaag gacgtgtttc caactatgtc tggggtttgg taggtgttct 82380 gacctacggt attacagctc taaatgctga tctttatggt aacgcagcac taaacctatt 82440 gtactacgtt cctatgcagt tctacggaat gtacttgtgg cataaaaata agccaagtaa 82500 tctaccggat gttccaacta aagttctgac aaaagctcaa gctttgctga ctctagggat 82560 tttagcagta gccacgttag gttatggtag tttccttgct actacggctg atccattccc 82620 aatgttggat gcatttactg cggttggtag tgtgattgct atgtggctta tggtcaaaca 82680 atatgctgaa caatggctga tttggattgc tattaacatt ggtactgtgt acatttggct 82740 tgtaactgct caatctacag gtgcatctta cgcaatccta gcaatgtggc tagtattctt 82800 aggcaactct attcttggag cttataaagg atactttgta cgcgttaaca aataaagtag 82860 ttatagccct ctaagcgagg gctattatac agaaattttc acttgcttaa tatagaaaat 82920 ttctgtataa tatatttata aattcgagag aggaatcaaa catgaaacgc attgtacttg 82980 cattgactct agtatctact tctgtatttg ctgctccgca ataggaactt tgtcaagttc 83040 aggtttgtaa taagttagaa cgatttactt tggatatttg gtcacacatt aaagataata 83100 tgggtgaaca gtgttttgat attgttctgc caaaagagca ggctgttcct ggcgctgttc 83160 ttagctcaga gtcacgttgg tggcaaggtt cttctatcaa tccaactaag aagtcagtga 83220 cacgtatcaa acaagtgtat cagtgcagtg ttcagtaaaa tttcacttgc ttaatgccga 83280 aattttctgt ataatatatt cataaattcg gaagattaac cctaaaggta agggagctgt 83340 ttgctaaaca gccagtagct gtggagacac ggtgtgccag ttcgagtctg gcatcttcct 83400 ccaaatttgg ttctcaagct catttggtat gagccgtcgc ctcataagcg aaaggtaggt 83460 aggttcgaat cctccgggag ccaccaatta actaggagac tatatgtcta gattctatga 83520 aatgaagaaa gcattagaag aatgtcggtg taaggattgt catggtggtg gtaggcagga 83580 tgatgcagaa cctggggata ttttctgtag agaatgggaa tgtcctacat gtaaaggtac 83640 tggtatcaac cctgaatatg caatttgtct agacattgag cgaggctaag atggatattt 83700 ttactactcc ggcaataaac cttttaggtg ttggcttgtt ccaagctacg gtgtatcgta 83760 ctgatgattc tactgatgtg gtaacgttca tcgtacctga gttcttccta gaaaagttct 83820 ttgaagagtt tgagcaattc cgtgaagaac acaatgctca ttccgatatg gaagatctag 83880 cagctatgtt ccctgcggtt tacggctata tctttgaagg taatggccta gatcttaata 83940 aatctgaact cgttgaactt aactggggca tcaattttga agttggctct ccattccctc 84000 gatatttcca gggtttagag attcgctaag attacatggg ggtgtaatcg aattggcata 84060 ggtactggac ttaaaattca ggttttgtgg gttcgaatcc caccacccct accaaattaa 84120 ggaaataaaa tgaaagctat taagaaagca ctacgtaaat ttatgtactg tcgttttcat 84180 agcatcaagg cttcaactaa tggtacgact tacgatcgct atgatcctaa gtttggctta 84240 cattctaaat aactggagat aataaatggc taatcctgaa attacggcac aacgctttat 84300 gctgaaagga attattgctg aagcaggcat gacagaagaa gttgcagatt ttcaaaagca 84360 gttcctaaat attctcactc aagccaaaga gctaggagag aaagaacatg gcgcggctat 84420 tatggcaatc acattggttg ggcttgatct ggcagaagaa tcaggagttt aactcctcca 84480 agcagaaatt ttaaaaatgt acttgctttt tacttaagat ttctgctata atagttatat 84540 aaattgatga aggagttaac gaatgtcaaa acgtgttact gcaactggtg ttgaaagtgc 84600 tgtttgtaaa tcctggggtg gctgggaagg tgagattgaa tggctttatt tctacgatgt 84660 agaacttctg ccggaagtca aagctaaatg tgtggaagct ggtatggctc ctgatgcaaa 84720 ggctgatatc gaaatcacca tgactaagct agaaggtcgt gtaattacgc tcaatgatga 84780 ctatgaagaa gtgttcgagc ttccattcaa actgagtgtc actcctagct tcgagtaact 84840 gatatagcac tgagcaagtt ctttaaaatt tgacttgctc agtcctttaa aacttgatat 84900 aatagttatc taatttggaa aacatatctg aatgagaagt aactaagcga gttgctttta 84960 ggggtaaagt caggatttcc tgaaatattc caccttgagt ttaaccgact tacttttcat 85020 tcaaatatgt tggattagta tcgtagaggt agcgaagcag actgtaaatc tgccgactcg 85080 gaagggtctc gggtggttcg actccatcat ccaacaccaa atttaagtcc tgttagacaa 85140 actggtaaag tcactaccct ttcaaggtag gatttgcggg ttcgatcccc gcacaggacg 85200 ccaatttctg cattcctaga gtgttactgg acagcatgtc ggtctccaaa accgtacggt 85260 ctaggttcga gtcctagtag gtttgccaat atttgcacct ttagctgaga tggattagcg 85320 cttgcctgaa gagcttgaga ggttggatca ttaccaacgg ggtgcaccaa tttgaaggaa 85380 atgctgatgc atcctgaaaa cttctttatt tactgtgcgg ataagtcact gaaacgcaga 85440 attaacaaag tctatcatta tcgtgctagt caacagcctt atgtttatgc ttttgacgga 85500 gatcacatcc cactagaaca gttggcctgt gattacatgg tattgaagaa ctttgacgag 85560 acaaagatct caagcgttca taatcgacag aacccgttaa cgtggaaata taagaagaaa 85620 aagaaaggca tcatatttct gaaaaatgta gttgcttaac cgcttaaagt tctgtataat 85680 tattacataa attgaactgg agaagcaaca tgacagaaca agaactgaaa gaacgtaagc 85740 tggtaatgct tcgcttactt aacattttct ctggtatgga tggtggtatt gacttcattc 85800 aactacgcac ttttgtagaa gaagcagaag gcgaggctat cactaggatt cttaaacata 85860 tgggtttgtt actagatgct gctaatgtgg ctactcaata taaactcgaa gatttaccgg 85920 agcgtgttaa atgaaaatgt tagttaaatt taagtgttgt ggttctgaac atatctttac 85980 acgtctatgt gagtttaaga tgtgtaagtg tggtaaatca ggctatgatg ctggagatgg 86040 ctattatgct agatctatag gacattctaa agatctagag tttactaatc tcgaaaagca 86100 agataaatcg accgttggct gaatggctta ggcggaggcc tgcaaaacct ccttatgtga 86160 gttcgaatct catgcggtcg tccaaatttt agggtcgtta gccaagcggt ttggcggtgg 86220 actgttaatc catgtcgaaa gacaacgtag gttcgaatcc tacacggccc gccatattct 86280 gtgtagagtt aagtaaaccg gtagccaacc agctatcatt actgatatcg aattcagtca 86340 cagataccat tttcattaat aactgaggaa atagatatga caactcgtgc aattgctcga 86400 ctagaagctg atcgtgataa tctgattaat aatattaagg ttcgtggtaa tattattaag 86460 gctctgaaag ctgaacttct tgaagaactt caaggtcaag tagagcgtcg tgaagctcta 86520 aaagaggttg aggaagctat caaaaaactc aaagctgagt aaacaaatta tgcgatcggg 86580 gctggcttgg taatggtact cccctgtcac gggagagaat gtgggttcga atcccatcgg 86640 tcgcgccaat ttaggatata gtatgacacg caaatattta gttattaaag gtgaacatgc 86700 aggacaaact gtatactcat tgaaatttca tgattatggt ttagcctcag atgatactcg 86760 atatactgga atagagcata caagtgtaac attagacgaa acaggtgatt atcctagttt 86820 tactattcct aaagatttct taaaagagat ttaatgcttc catagctcaa ctggtcagag 86880 ctaccgcctc ttaagcggag ggttgaaggt tcaagtcctt ctgggagtac caaacaaata 86940 gagtaaaggc tacagtaggt gcacccttac ggactgctac accggactct attcattaac 87000 aaacattcta gcgtggtgga gaggggacac cagcttgccg agggtagaag ctcgttcgat 87060 tcgagtgaaa gaatggttct cgtaaatccg cacaagtgat agcaggttca agtcctgcct 87120 agattgactt attatgcgtt cgtagttaaa aggtataatt tttggttgcc aaccagaagt 87180 tgagggttcg attccctccg accgcaccaa atttaggttt ctgcttccta gagaacggac 87240 tcctagataa acaaggcgat gagtttccgg ccggttctag tcgctgaaaa tcctcccgac 87300 caggtaggtg taggtaaacg aaagtttact tattgagtag gttccgatag atcggcgggt 87360 ctattctggc atttaagaga agtctcgcaa acttctttgt cagcagagcg tttgaagcgt 87420 gaggtggcct acgcaataag taaatttatt aaaaactcac ttgctattat gcttaaatat 87480 ctgtataata tattttataa agtgagttaa caaagaatat tctccgtgta gctcagtttg 87540 gccagagcgt tccgtttggg gcggtagggt cgggggttca aatcctccca cggagaccaa 87600 acaattgcgg gttagatctc tggtagagat cgctagtctc ataaactaga aagaggtagg 87660 ttcgattcct acacccgctt ccaatttaat agaaggattt cgaaatgaaa ttatttcgtt 87720 taaaagtaag tgatctacgt tctagcaaag atcgtaagaa gtatccacta gcccattatg 87780 gaaaagatga aaacgaagtg atggcggaat ttggtattaa tcatccttac ttgaaagtct 87840 attctattaa agaagtatac atgtattaaa ttgaccgtta actcaactgg ataagagtac 87900 cgagctttta cctcgtaggt tctgggttcg aatcccagac ggtcaaccaa tttaaaggaa 87960 tttgttatga atattaacaa agttctagaa acaatatcag aaatgtctgg cagagccgga 88020 aaacgcttta ctgaactgtg tattaaaaat caaaaagtac cagaacatca gtgtcattat 88080 tgtcgagact gtgaacaata ccatgaaatg tgtatggttg aagattggat ctgggatcag 88140 ataagaaaag agcagcccaa agatattgtt ctatgtttca cttgtatgga acgacgctta 88200 ggacgtaaaa ttactcttaa agatcttacg catgttccgt gtaatgcacc ttacttcatt 88260 ggctttagaa tggagagatt ttaatgacta tctggtttta cttaatgaag cagaaaataa 88320 aagattccta tgtattctct gccgatttta caataggtat gtttttagca ggtttctgtg 88380 aatttgtagt tgaagcctat attataggac gtcttttgct cggttagttt aatgggagaa 88440 ccccgtcttt acacggcggt tgcgatagtt cgattctatc accgagtacc aattaactca 88500 aaataaggaa ttgcaaatgc aattatcaag caaggttcaa ggctatttcg gtcatgtaac 88560 aatggagttc gctatctcta aaggttggga tttcgagcaa gatctagctg agttctacaa 88620 agatattgat ctgaacgtac catacatggg actctccact ggtcaagaga tcacagaagg 88680 cgatgtgttc aaggcaagtc aaggtgaggt aacacctagt tttgccaagt tctggttagg 88740 acttactttg aaggaagatg caccagagga tggtgtggtt atgcagagta tgcactctgg 88800 aagctctgat taccttttcg ttagtgacgt taaggaacta caagaaatcg ctaaaaatgt 88860 acttgcttga tgcttaattt cttgttataa tatttatata aattgatgag gagaaatcct 88920 catcaaagtt cactaaggta ttagctcgac ctaggactgg ctgccaaaaa gcacaattgc 88980 aattagaccc ctaatggcgg gggatacttt gcaataagtt ctagattagt ccactagatt 89040 cagcggcaac tgaatagtgt atcgggtttg tgaagagcgg ttatcctcta ctggctaatg 89100 tctgagggta atacgcacac cgagctaata gccttagtaa acttaaagat tttggggata 89160 tagtttaatg gtagaacatc tgctttgcac gcagaagacg agggttcaac tccctctttc 89220 tccaccaaaa ttcggggtca taggttattt ggttaaactt actgccttgc aagcagtgga 89280 actcagttg attctgagtg actccaccaa acaaatgggg atgtggcgaa attggcagac 89340 gcgctagatt taggttctag tcttcgggtg tgggttcgag tccctccatc cctaccaaac 89400 attggattaa ctcatgatga aaactcctat aactgatatc tggttagctg ttaatacatt 89460 aactaaagag tatgtgttta tagagtatgg tcagaggtat gaccaaaaga tgtataatct 89520 catacttcca gtatctgctg aggaagttcg tgatttaatc cttaaaaatt cggaagagca 89580 aatcgattag gtgacgaaaa ccgcttggaa agtggctgac tggtaaaacg gcttgagagt 89640 tcgaatctct cttcttccgc caaacaactg gaaggtagga catagtggta tgtaacaggt 89700 cttgaaaacc tgcccgctgt agcgatatgg tgatggttcg actccattac cttcctccaa 89760 ctaataagga aaatcttatg aaacacattg ttaagtatac cgaaagcgaa cgtggctggg 89820 gtggtgaaat ctggtatcgt ccatttgaca ccgaggccga agccttgatg gaagttaatg 89880 atgtaaataa agatctacct aaagatcatg tgccaacata ctacatccaa gctgagtata 89940 ttggtgttaa ggataaggtt cccgaaggtt acaagtttta aataattctt gaagtatgcc 90000 ggaatcggta gacggggctt cctaaagggc gtaaggggaa catccactcc tcataagata 90060 cccgaaaacg aggctgagat aaatggtcta tctcgtgaag gttcgagtcc ttctgcttct 90120 tgatattcaa tggctcaaag agtaacggtg cacctttcta gagaatttcc tgggcgagaa 90180 tccaggtgta atcaactagt tgttagtgtg atgagtttcg gagagccgcc acaattgaaa 90240 attcgtactc gtcttgagcc tcgtgcaata ggagataact tgattagtat tctagaaaat 90300 gctatttgag gatggtttcg ctaaccctgc gagttttcaa ttgtggctat atcataattg 90360 gttaatgatc ctgattgtga atcaggccta tgtggattcg aattccacta gccaccccaa 90420 atattgcgtc cttatttcaa cggaaagaat gtaaagctac gaactttacg atcggggttc 90480 gattccctga ggatgcacca attaacttaa acaggaaata aaatatggct gatttctgca 90540 aagaatgctc tatcgatatg tggggcaaag atacaggtga tctttctggt ctgattacag 90600 aagccgaagt taaagaaggc tatggtgctg ttgtaatctg tgaaggttgt ggattattc 90660 gtgttgatca taatggtaaa cgtctagaag aaccagatcc aattgccacg gttcctgtag 90720 aataaaaatt attgggatgt agatcaattg gcagatcgtc ggcctctgac tccgaaggtt 90780 ccacgttcga tccgtggcat cccagccaaa tacttggaga gtagtgtaac ggttagcaca 90840 acggcctttg actccgttaa tggtaggttc gattcctcct tctccagcca atttatgcgt 90900 gattagttca gtggctagaa taactggctt ccaaccagta gacacgagtt cgactctcgt 90960 atcccgcacc aatttaagga aacgttatgc acgtttttga gtttcgtaat aaagattcac 91020 gtaacggtgg agttgaaggt ttacgtacct taattatagg gaataactac cgagcttatg 91080 aaaaagatga tggttcagta ctcctaaatg atgaagaacg tggctatagc ttcttccctc 91140 aagaaactta tgaagagttt cgtaaacaat ttagtctaga ctggagaaag ctagttacta 91200 gcagttcgtt acctgcttct atcagtatga gttcactata aatggctgat aatctaaagc 91260 taaaagtaga taaacaaatg aagtgcgttg aggttgagtt gggtgaagaa cgtttaaaac 91320 tctccttcga attcttacaa cagttggcta tgacagtaat gtctgataag tctcctgtat 91380 ttgaaaaaga aattccgttg aaataacggg tttattgctc ctaaagcatt gctggcgatg 91440 cagttgcctt gtaagcatct gaaccgggtt cgattcctgg tgggagcacc aaatttaaaa 91500 attcatttgc cttctctctt atttcttgtt ataatattta tataaattaa tgagagggaa 91560 accaaatgaa acgttatctt tctgtcgtat tccaaacggg tggtcaacgc tacacttacg 91620 aatttcctga gtcttggaaa atcaaagagg gtgatcaagt tgttgtgcta acacctcgtg 91680 aaggcttcaa agtagtaacg gttaaacaag tatttccaaa agatcacgaa ccatctaagt 91740 ctattaacta taagatgatt catggtctgg tacgaaaagt acctagaaca gaagtcgaag 91800 tagataaaac tggagaagct aaataccagt atacctctta cttagacgaa atgatgtaat 91860 gctctgatag ttcaacaggt tagaacaggc gaccgataat cgtcaaatct tggttcgatc 91920 ccaagtcgga gtaccaaatt atagttagtt ggcagagcgg ttatgcacct ccttcatacg 91980 gagtgactac agtggttcaa atccactact aactactaaa cagaggatca ggtcattgta 92040 ccagatcctg ctgagaagag agtccacatt ctataaatgg ccaggcgtga cagcaggaag 92100 tagactgcaa ctccatgcta tccaggagta taaatgtaga agctaagact ttagatagcc 92160 attgtcttag cgtgagcaat cacgtagttg caacagacca ggaggatcgc aacgttaaaa 92220 tcaaagctcc tgtgcttatt tatctaatcg agaccatcaa tctcaccgga atgctagcac 92280 cccaacagta gctagtgatt agataataag caagcctgcc ttccagtttt tatcttgctt 92340 ctgggttaac aaaaagctga gccaaattct aacatcaggg agatctaatg aaagcatatc 92400 aaagtcttaa gtctggcgta attaacattg ttcataacaa tcagatcatt cagctatatt 92460 ctaaagatgg tgaacttaaa cagaaagttc tagtcgaaga tctagaaggt atcacacctc 92520 actttgaccc tgaggccttt cgcattgtag atgtggaagt ggttcctcaa gtcgagggtg 92580 gtcagcacct aaatgtcaat gtgttaagcc gtgatcagct tttggatgca cagaaacatc 92640 ctgaaaaata tcctcagttg accatccgtg tctctggcta tgctgttcga tttaacgcat 92700 tgacgcgtga acaacagaac gacgttatta gtcgtacatt tactcaggtg atataatggg 92760 gtttggatac gatttctccc agctacgcaa ggtagtcgag caaactccat ttatctccca 92820 agttttagga gaaatgtgtg gatcgagcat taagacgcca tcaccggcaa cgtgtgaaaa 92880 acaatcgcaa gaagtattgg acagtcttcc cacatgaaga aagccctaag cggttaggca 92940 ttatcactac cactccttgc atctgttctt gttggatgtg tgggaatcct cgcaaatatt 93000 ataagaatag taaagcaggc atgaaaatct cggagattaa aaagatggat gcaatgatta 93060 tgggcatcac ctttacaggt ggtatctctg atgaattcga cggttttgta actcaggata 93120 ttgtcgaaat tatctaatta ataataaacc ccagctattg aataagtagt ctggggtttt 93180 cttgtatgta ggattttcga aatgaaaatt tttaaagtga actatagagg cctgtggtta 93240 ggtggtacag ctattgttgt tgcggaaact gcggaagaag caatcgaact ggctagaaac 93300 catagaagtt ctatagaatt cgaggatgtg acagtagaag aactcccgag cagtggggta 93360 gtatacaacg acaacggaga ttactgatgt tatattttga agttcgccca cattaagtta 93420 atttaaaaga cttaatggag gtaatatgag ccgtacatat cgtaaacaat ctggtgatcg 93480 tcactggcgt gatgcacatc gttctactta taattttcat agcactaata tccgtattcc 93540 gtatatctac acggataggg atggtaagca gtggaagtat tatcaagttc gatccaatga 93600 agcatgggct aaaacttggg atgaaattga gaaggagatt caggaaaata tcatccgtaa 93660 cgagaagaaa gatgggcact ggtgggattc tgttagtagg aatgttaagt ggcattctaa 93720 caaaatggtt cggcagggaa accgtcgcga acttcatcgt gtgatgaaag atccagaaaa 93780 ttacgactat aatcgcgatc atgatctgcg taagcgtggg ttatggtggt gctacgacta 93840 atttcgtaaa taattcattt gctaaaaccc tctccttttg atataatatt tatataaatt 93900 gatgagaggg ctttgaaatg aaaactcgac tagactgtgc tgttcacgta cttgagcatg 93960 atgttgttgg gacttgtatg tcttatcatg aagcgggtaa aatgattgtt aaacgtgttg 94020 ctgatgtttg tagtttgaaa attacagaag atgaagcaga cgaactactt ggacaggcaa 94080 tagagaaaac agaaattttc ttgtgcgatt cttgttcttg gtggtgtgaa gctcacgaac 94140 attctttcaa tgattatgat gtatgccgtg actgtaaaga ggacgatgaa gatgaataag 94200 tttattattg ctctagtaat ctcagcagtt agtttcggtt ctctggcatc taccaaagtt 94260 tctatgaaaa atggtaatgt taaagttcag cagaacggca ttaccaccga atatggtaaa 94320 gtacgtgatg ttaaagaacg taatggcaaa gtagaaatct atactaataa aaacttctct 94380 actccggcta ttactgttag taaacgtggt gaaatcacta ctcaacgtac taatagttct 94440 gattctttta cttgccgtta tgactgcggc tttgaaggtg aagatgagta atttagaccc 94500 tagaattaaa catgcagtcg aagaaatcca agatcagata gatgaggact tcactgtttg 94560 gtcaagattt ggcaatggtg aaggctgtca gctttatagc atgaaatgg gcatctcgat 94620 tgaacttagt atcaatccag agggcaacgt agaagcccaa cccatgttca gtgttccagg 94680 cttctctgga tttgttatgg gcatgaaatt atgtttacca aataaacatc tttaccgtgt 94740 tgtctgccag ctagaaacga ttaagcattt cttgcctgaa ggtaacatta acgactacta 94800 ccatgaagtt gtagcagctt acatgatgaa agaacgcaaa cgtcggagag aagaacgtga 94860 aagagctaaa tctcaacagt ctagtacaga ttccagcaac tgatgctgta ctagacttcc 94920 tcaagcagga acatattaag ttctggacta actatgcaga acagtacgaa gaaacttcta 94980 gtgcaataac attcgcggat aaacgaatag ctgagtatac agattctaga gttaagaatg 95040 gtgtgattac tttaccactt tgggatgtga tggaaaagtt tggtaaagat ttgtgcccag 95100 gttgtgtacc attgtttgtt actattctaa ttgatgaaaa ggatcttaaa tgattatctc 95160 tccatttgct ttgtggtttg ttattggtgc agttgtcgcg atttacagcc tgattgatga 95220 tctgtatatc tctaaaaata aagatgttat tctttatgtt ataaacaaat atcgtccacc 95280 agaattagca gaatttgacg atgaaaagat gttcaaattc attatgtcag gtatgcttat 95340 catggacacc ttgttcgggc cagtagctac ttacttcttc ttccgtaaaa ctcgtaagat 95400 gaaaaagttt cgcgctgaaa tgcagagcaa gtaaagaact cgcgtaggac cgagttgcgt 95460 cacctgcaac tgttacggat aagggagtcg tgccccttag tgcggtaagt ggtgagttgg 95520 tcgctgacct caactttaac tggagatatc atgagcttta ttttattcat tgtagcagca 95580 tggtttatta ttggtttagg ctatgctatt gcagttattc gtcatctgga tgaatattct 95640 gctgaatggt ttattaagta cctacgttta gacgatcaag aacacgagtt cgacagtgct 95700 gaggaaagac aggctgtcca gtcaatgact cccaaagaat gcttgcttta catgcgtgta 95760 attggtttct ttggactgtt ggtagcgggg ccgctaggtc ttactattgc tccctacaaa 95820 gaagttgttg acgacattaa actatggaga accgctggat cgctacgtcg tgctagagaa 95880 ttaagtaaac aagagtgatg ataatcacct cctagcaaga ataataatga caaagcccag 95940 cctgcaattt tgcgactggg ctatttttgt aataaaattt cagttgctca gatgccacaa 96000 gtattgtata atatgtttat agattgaggc aaaaggataa tttatgagaa tcatttcaaa 96060 gttcaatgat gtatacgatt tgcagaatag tttatttgat ccagatcgtg tctgggagcg 96120 taaaactgaa gagctgctag taaaagtaac cgacgatgta gaaaagaata ttgttcataa 96180 tcgtcaggtg tttcgtgaag gttccttatc tttccgtggt gatttcgaat actcagtaaa 96240 tccgctgttt attgcaggtg aagtttactg gttacatgag ctgtcctgct ggcatccggc 96300 attcagtttc aaaacgttca acatcgatgc tgtatttgat aaaatggaag aaatgggatt 96360 acatgcccgt tcttacttac tagacaagaa tcgtggtgat gttcgtgaaa ccatgcgaga 96420 tatgttggtt gaagcaaaac ctaaagcaga acgtatctta tctgaactgc gcgtaccaat 96480 tgcatatgtg aaaggtatta agaagaacga taacgatgat gttcgtaact tcgtaattca 96540 gacaaacatt cgtttccacc agtcaggtat tccgtggcag gaaattgaaa gtaacttata 96600 ccgtttacac caagttctcg aacagtatat cttcggtgta ctaggaactg gtgagcctga 96660 tatgattacc gtatcagata aagacaggtt agcagcacat ggatttgata caaaaacttc 96720 tttcaggaat atggcaaggt aagtggtata tcctgggagc aggtctagcg ataggcatcg 96780 gattcggtgc ttatcactta gtaaataagg ttgagactct agcaggtgat cttgcagtag 96840 ctactaaaaa gattacgtcc ctagagactt cccttaataa tgtgaaagct gagagtgaac 96900 ttcgtgaaac tcgtatgaat cagtatttca caatgaataa tgtttcccaa gcagatctag 96960 acaagaaaat tagtcagctt gataaagcac ttagtcgtca agacattata tctgcaaagc 97020 ctggattggt tacattaatt gctaaaaagc agagtaagga gttcgaggaa agattagcat 97080 gtttaactgg aaacttggag tactgctcgc agccgcaatc acaatcacag gctgtgcaga 97140 agaaataaaa ccagagcctt cacatgagtt aaagcaagcc catgttgact ggcctaaagg 97200 acttcagccg tgtagctttg atttcaaatt cgaaaagaaa ttagctacaa atggcgaaga 97260 tggcgtagta gttgttgtac cttacaaaga ttggaatgca aaagcaaaat gcgaggaagc 97320 agtttactct tacatttcac gactgactgg tatggtttgc ttctatcgtc aagatttaca 97380 agaaaaacgt tgcttagttt actatccacc cattacaaat aggaaagatt aatgtcagta 97440 ttagtcggtt tacatggtga ggctggtgcg ggaaaagata ccgttgcaga attaatgatt 97500 gactggtgta atgacacgtt cccaacgtgt ttatcccgtc gttacagttt tgctaaaccc 97560 gtttacgaac ttgcatccgt aatcctcggg gtaactccag agtttctggg agagcgcagg 97620 ggaaaagaga ttgaccaatg gtttacggtc acacaatctc aattggagcg agctagagac 97680 gtatggttt agtatggtat cgataagttt gaggacttct cgtacgtttg gccgattttt 97740 gaaggaaaat accttgatcc tcagcagtta attgggcaag ctgatgatgc gctgtatagc 97800 gtatttatct ccccaagaaa agtgctacag cttgtaggta ctgagttagg aaggcagctg 97860 gtgcatgaac gcatttggct cataattctg gagcaatcca tcgataaaga cgacccagac 97920 gtcgcaatcg ttaccgatgt gcgttttccc aatgagggag agctaatcat ggaaaccaat 97980 actctagata tggattccat acttattaag gttgaatctg gtattactaa taagtatcgc 98040 atcgagacag atcatccgtc tgaaagcggt attcctgaaa aatacatttc tcataaattt 98100 gagaatacct tcaacggact tgatgcactt cgtactgatg tataccactt ctgtgactta 98160 gagctagaac cactagttgg ataattaagg atcactatga ctaataagaa agaagaaaag 98220 actaacctgt ttcacactgt tcgccaatct aacgaatata cctttttctt tgatgaagaa 98280 ttaggctcac cggatgagta tcgtgaccta tccatgatac tgatgcaggc tggtgaagat 98340 gatgaaatta atctgatgat taatgggccg ggtggttacg ttgatactgc tgcacaacta 98400 tctaacctaa tcgctaactg tcgtggaact gttattggac acctactcgg tcctagtgct 98460 tctgcttact gcacaatctt cctatcttgc cacggttggg ttgtacatcc acatgctacg 98520 cttatgggac atacgttctc tggcggattt tgcgagaaag gtcaagaaat caagaaagcc 98580 tatgaatcct acaaccagtt tgtagaagat atgatgctag atatttactt tccgttcttc 98640 tctgtcgatg aaatcgagga catggtaaaa gagaataaga atatctatct caacagcaaa 98700 gaaatcaata agcgtattga aattttggcg aattatcgta aagagcagtg tagtaaagcc 98760 caagccccgc atccagaaga acatggagaa gtgtaagtag ttttaagcca gagtttaacg 98820 actctggctt ttcttttgcc taaaattctt cttgacaacg acaaaaaatt gtgctccaga 98880 agtgatctta aaaaacacat tgtaatttta ctctagaatt agtataatgg tagtggttag 98940 aggaggttac aagtggaaaa gtttatacaa ctattaacga tactgctaca agaagcgaaa 99000 gatccagctt cgctactaaa acgtctgcta actatcttag ttgctgtcat tattttcttg 99060 tttgtaagta acactagcga ggtgatgtca tttttaaaga ctttctccac gtcttcggtt 99120 ttacaggatg ttcaaaccca aaggatagat aactttccta atgtagcacg agaaaagagt 99180 atggttcttt tctcccagac tggtgcggat gccgtttttg tagtaaagta taaaccagat 99240 gctataaatg attattcaaa cattattgca tgggaaagca atgcgcaatt ggatagggct 99300 gacttggccg ataaagcagt aaataagacg tctgaacttt atagacgtca cctggaaggc 99360 tttaactacg ctgcggattt aagtgtaaaa gtaaataaat atactgggtc aaatatacca 99420 gcatttaaga acattacttt taattacgtc tacacttgtc catatttcaa cctaaataat 99480 atctatgctg gatacatagg tattgcatgg aaggataaac ccgtagatac agctgattcg 99540 gaacaattta atgagtactt agcaaagctc tgttccccac aacggagatc attaggtaga 99600 tctatatgag ttttaaattt ggtaaaaata gcgaaaaaca attagcaact gttaagcccg 99660 agctacaaaa ggtagctcgt agggctttag aactatcccc atacgatttc acaatcgtac 99720 agggtattcg tacagtagca caaagtgccc aaaatattgc taatggtact tcattcttga 99780 aagaccctag caaaagtaag catgtaactg gagacgctat cgattttgca ccatatatta 99840 atggtaagat tgattggaaa gatctggaag cattttgggc tgttaagaag gcttttgaac 99900 aagccggtaa ggaacttggt atcaaacttc gttttggtgc cgactggaat agttctggtg 99960 attatcatga tgagatcgat cgtggtactt acgacggtgg tcacgtagaa ttagtctgat 100020 aataaatcaa ggcggggtaa ttcccgcctt ttaaagcaaa gggggcttta aaaacttaat 100080 gataggagaa agccatgttt gcagaactat tcaccatgat attactagga gtatggaaaa 100140 taagcctagt aattttcatc ttaatgataa ttctcactgt tgtcgcacta gttacccaaa 100200 atgatacatt aaagaaagtc gttcatgggc tagagtatat aattatgggc acattcggcg 100260 tttgcaaatg caactgccag aggaatacga aatattgttg gctttggatg gagttagaga 100320 actgcatcac agtagccctg gctgtctcat tcggtatgat tcttatggcc cttactctag 100380 cattgatacc actaatgtta gctggggagt cacggcttat ttcacccttt tctcccctat 100440 acttatgtat tcaatatatc caataactat gtacttagtt aggaagagat tcccacatca 100500 agcacactaa tataaaaaag tagttgactt tccgctgaaa tttttatata atatttgtat 100560 aaattgaatc agaggagtct cacaaatgtc agatcgttac tatactcaga tggctaacca 100620 ttacaatatg gctccgtatg aattgaacat cgcattacgt gacctcgatt caccagagcg 100680 tgcaaaactt gagaaaaagg cgggaattcg tatgtctagc aaaggcaaga aacttactcg 100740 tattgactta aataccatgc taatggaaga gcttggcgtg aatattgaag gtcagaagtt 100800 gcctctcaat gttcttgaaa cgatgctgga taaagtcaaa aagaaaacct ataagaaagt 100860 acaagtaccg gaaggcaggc taaaagcccc ttatcaggct gctgtatctg agtgcttagg 100920 tgctacctta gatcttagca ctgcaactgt taaagtgatg cgtgcgtttt tagaggcagt 100980 taataaattg tgattaccca agaaagattg aaggaacttc tagagtacaa tccagataca 101040 gggatattta tatggaagag ccgaaaagta gatagaagtg gttttaacaa gcaatttgca 101100 ggaaaaattg ctggcaatcc tcaaaataac ggttatatca atattactgt tgattccgaa 101160 gttcatgggg cacatagatt agcctttctt tacatggaag gctatatgcc tgcaagagta 101220 gaccacgaaa acagagttag gtctgataat aaatggctta acctacgaga agctacagtc 101280 tctcaaaatg gtgctaatgc gttaaagtat tcgaacaata gttctggata taagaacgta 101340 tattggacaa agaaaacatg tagatggcaa gtacaggtta tgaaagatgg taaatcctac 101400 tcgggaggat acttcacaaa cctcaaagat gctgtagaaa ctgccaatga gttaagattt 101460 gaattatttg gggaatttgc cctatatgaa ccatttaaag gagataaaca tgagtaaatt 101520 agtatatcta ttgaagggat ctacctgcaa tccgtgcaaa atgttcgagc ctgtatttga 101580 taaagtagtt caggactaca gtctagaagt tcataaagag actgataata cagaacttat 101640 gcagaaattt ggagttcgtc aggttcctgt agtagttcta gcagatcgtc tccctaatgg 101700 tcgtgtggaa gccaaccaca ttctgattgg tcgtcaactt cgtaaagaaa ctatgcatga 101760 cgcaatcaaa aacttcctgg aagataatcc agaggattaa tacccaaagc ctagtcttag 101820 ctgactaggc ttttttatta cttgctttct gctaaaaatt ttgatataat atatgtataa 101880 attgatggga ggagtatcac attgaattac gcatatctta taatacataa caacgtggtc 101940 tgtggtactc gtgctgtaga aatgggaatc acagaagaaa aatataagtc actgcctgaa 102000 aaggagcaag aatattatgt tgagcaagct gcttgggaat atgctgaggc ttatcccgaa 102060 gaacgtgaag gccgtgttac tattgttgtt actttggggc tggtgggctg tgataccgaa 102120 gttgatacag atctagaaac acttgaagaa tgggaagagt tggatattgc agaacaaaat 102180 gctatcatcc gtcaatcttt ctgggaagca gtagactgtc acgttgtctt tgagccaaac 102240 gatacagaag ctgaaaaaca cactaattgg atgactcgat aatgaaaaaa gaatttaacc 102300 tgcacgaaat gttagtagta ccagatgatg ttaacctgtt cttcgtagga gatattcacg 102360 gctgtaatga tatgctggaa gaggctctta aactagcagg gtataaagaa aagcgtgact 102420 atgttgtctg tgttggcgac ctgatcgacc gtggcccgca gaacttacag gttctagcca 102480 aattcttata taacccacgc ttccgtagcg ttcgtggcaa ccatgatcag tttatgattg 102540 ctggtgacta cgccaactgg atgtataatg gtggaatgtg ggcaatgaat gatctggata 102600 ccgatacaat taaaggtatc gcagaagata tggcagctaa aatgcctgta ttcatgaccg 102660 tactccatcg tggcaagaaa tacggtgttg ttcatggcgg aataccgttt cagtataaag 102720 atgctggatt cgatgtacat acacctaatt gggatgatat tattgatcat atcgctgcaa 102780 gtgaaaatga tcccaatgat catcctagct attacattga gccgtatctg tgggatcgtg 102840 atgtaattca ggaaattggt tttcatctgt ctaagcaagg tgcagagcat ccctatttcc 102900 agcgttatgc aggatttaaa gatgaattga ctgtggaagt accagaagtt gaaagtgtag 102960 atttcgtatt ccatggtcac actggtgttc cttacccaat tctgtacaag aaccgtgttt 103020 atctcgatac tggcggtgtt ttcaacgggc agttgacggt tgcgcaagtt aatgatgaag 103080 ccggtaaaat catgacattt accacagata aaaatgatag ctgtggtgta cagaggattc 103140 tataatggaa cgtgaagaac gttatgtagt tataaagcta tctgatattg agaaagccat 103200 agaattaggg catatcactc aaagtaactt tactactttg gagcatatct tgtccaaact 103260 atggtatagt cgagcacaac gtggtaaaga ggatcttaaa acagtcgtag tagagcataa 103320 ttggccagag tacgaagaag tctggaagat gctagagaat cgtgttgaat ttgaacaagc 103380 acgacaggaa gaggttgaca aaggctacca agaagcgaaa gagcaccatg aggtttatga 103440 tgaaatattc aaacttcgtg gtattagtag ttattgtcag ggttggaata aatatgcgga 103500 ggaagttctg taatgaagag attactacta ttatcagtac taatactagc tggatgtgat 103560 tcctacgacg ataatatgag atttactgca gaagatacaa aacacttatg tgatgtagct 103620 ggaggtgagc tagattattc cagtattcaa attggactca caaaaggtat attctacgat 103680 agttatacta ttacatccac ctgtgtaagg aataaataat gaaagttaaa ttctggagtg 103740 acctccactt agaccaccca gggattcttc gttttcgccc ctggtttaag aacatgaaag 103800 agcacgatga gtatatccta gatactttgt ccaagcacat tggcaagcgt acaaaacttg 103860 tgctgctagg agatagtgcc gtgggggcga gaagggttgg ccaagttgaa gtcggttatc 103920 cacaacgtgc ctaaccaact aattatggga aaccacgatg ctgagcgtca gggtctgcat 103980 attaaagatc ttgtagacgt ttatgatgat attcaggcta taggtaagca aacaggtggt 104040 ggcaagaagt tttggctgtc acattgtcca ttgcacgaag cagaacttcg tggtaggcta 104100 aacattcacg gacatgtgca cactaattct gtgcgtgacc cacgttatat caacgtctct 104160 tttgagatga gcaagactcc tattcctcta gaggatattg tgtctggcaa attcacaagc 104220 tatgataaag tggttaactg cactactggt gaagtactcc aaatcgatga agatgtggca 104280 gcggtattga ggggagatat caatgtttga ttttgatggc atggctcgtg agatatttat 104340 agcaataggg atggtagttc ttatctcttt ctttacgggc ctaggcttag gccttttatt 104400 ttaaaaactt catttgctaa aatgcttagt tttctgtata attactttat aaattgatga 104460 gaaggaatca aaatgcaaaa gattgataaa gcacttttaa tcgcaacagc agataagttt 104520 gagcaagtta aatctacttt taaggcagtc tttcagtcct atgttcaaga caaatcgaac 104580 ccgatttctg agcgtttgat gatgtgggaa tgttttgctt gcaatgctct gctaatttcc 104640 gattataagg gagaagtatc tgaggaattg catgagatct ttattaatga ggatactcca 104700 cgctatcagt tagtgaactt ccaagatcta gctgagcaag ttattcctga tgatctttgg 104760 gacaagtatt acggtgatcc tgaagaagat ggtatggaac ctgagaagtg cattgaactg 104820 atctgtaaag atcaccctga aattgctgag aaatttgagt tagtatttgc ttctgagttc 104880 tcaggtttcg ttaacgattg gtaagtaaat aaaaaattca gttgctttta gctttaaaat 104940 tctgtataat aagttcataa attaatgaga gaggaaattc taatggaaaa catgactaaa 105000 actgaaatgg ctaacgttct ggcaattctc ctcgatatgc agggttttga aggtcagctc 105060 atgaagatgt ctattccggc aatgaaaaag atgtatgatt ctctgaacaa gaatgctatg 105120 gcattcaacc ttgcgaaaca agaagcacgt tttgcgaaag agcatcaggc aactgccgaa 105180 cgtcgtgcag catcttttga gcgtgaagtt aagcaactga aaggtaagaa ataatgacta 105240 atggaaaatt cttacttgta ctagggctga atgccctagt ctccctagct atcacactcg 105300 gtgttagtga cctcatgctt aatcagcatg agaagaaaga agctcttgaa caagtgtaca 105360 ttagcccgga aatcaaagaa caagcaaaac gtaataaaat gctctcagaa gcaaaccttg 105420 tgcttaatca agcagagttt gatatagctc tctcaaaata ttccggtgct gaagtaaaag 105480 atgcacacgt tatgaaagac ttggaagaaa taaagcatca aattgaagag gccaaaagat 105540 tttgtaaatc aattgaccaa tctttagaaa aaatcacgtt ttatagaact ataagcccat 105600 atattgaatg tggcaacgga gtatttaaat gattacagaa attttaattg gattgctggt 105660 tctcacgact ttagcagcaa ttggcggaat cattggcatt attaatgcta ataaaaatat 105720 ggataggatg cgcctcacca atgatgggct agttaaacgt ctcgttgaac aggaagaaga 105780 tatcgaatta gcacaaagac gctcagatct tcttaaagaa aagctaacga atattgaaac 105840 tcttgttggt aacactaaac ttcctattaa agtcctgcgc gctcaggtaa tcacggagat 105900 taagaaatga tgttaattat cttagctttt tatctggtta ttgttggtgt tctggttact 105960 aagtatcata cctggacccc gaagaacgtt ggtaaagtag cactgtttgt tctgccagtt 106020 cctatcatca tcgttgcaat gctgttcacg atgttggccg gtaaagtgac aaaaactgac 106080 gttaaacgca ttgtagacga actacagcaa tcttctgaca tgatcgagga tattctgaaa 106140 gatgaagctt aattttagtg aattaaccct gctggacgag tttctaggtt ttgttactga 106200 acgtgggttt attaatgtag atcctcagac actagaagtc ttgcaatcca tccagaataa 106260 aactaaaaca gatctggaag acttatggcg accactttct gagctaactc ctcttaatat 106320 gaaagtgatt gttaaaaata ttgaaactgg tgaagaacgt gagatggttc gtaaagaact 106380 ggctaatagt tattctccag agtccctagt gatgcatcat gacgatgaac cagaaacact 106440 ttggacagcc cattatattt ggcgtctgcc ttgattgatt taacaactgt tacggcaatc 106500 ctaatcttcg gtttagtggt tgccgctttc ataatcgttg agcaggccaa agtaatacgg 106560 agacttaaaa acagatgatt aatttttata accctaactt gcagaaagca acgtgggatt 106620 actttataat tctaccagct tttatgttaa gttgtgcaat cattaacatt atgtatcaag 106680 aagctggttg gacagtccaa gatttccaca tttcacaagt tcttcctttc atgtatgaag 106740 gcgttatcgc agcaataaat ggggttcgta aatgacagca ctgcaaagat tacagaatta 106800 ctggtttgat ggccaattta atgatttatt catccagttg tttgtcgaaa cgaacggacg 106860 ttttaattac cgcttcttta atactttcca tgaaacgaag ttctcgcata cagagattaa 106920 cgcagcgatt caagatctca ccggatctaa ggtaattcct tatcgtgatg tagattttgc 106980 acctgactgt tttggttttg agctgttcaa gaaagcgtat aagttcggta agtttgaaaa 107040 tgcacgacat tgggttcatg atttctggta taataccgat atcgttccta gccgtgtact 107100 gatcctcaac tggattgcta aacaacatcc gccgaaggca caatcggcat tccttccaac 107160 tgatacagga aatctttacc atgacacaaa agaaaaatcc agtgctagag cagatgaagc 107220 agttggagaa gcagattgag gaaggaagcg ttgatgggca gtcaattgtc aatcatgctt 107280 tcgatcttac agtaggatgt gctaatccgc tggtggcggg tgaaacagca gatattcttg 107340 gacttttaat ggcagttggc cagttaattg agattaccca agaagagtta gacaaggatg 107400 aagaagtaac tattcatgat gttcttggtg ctctagatct gattgttaat gcttatgcct 107460 gcaaacgccg ccactcaatt gatgaatatc tagtttctcg tgagattact gaatctcttc 107520 tggaaactgc tgatatggcc ttaaacggca agcaacttca ctaaggaaat aattatgcaa 107580 tttaatgtta tatctgcttt tccagggtgt ggtaagactt actttactaa gtgttttcca 107640 cgtgtactag attcggattc ttctaaattc ccgaaggata actttccagc gaactatatt 107700 gagcacattt cacaagctat agaagagggt aaactgatct tagtttctag ccataaagaa 107760 gtacgagatg ctcttcatgc tgctggaata gattttattc tgctttatcc agaacgagag 107820 ctaaaagaag aatatttagc taggtacaaa gaaagaggaa gtcctaatag ctttatctct 107880 ttacttgata gtaaatggga cgaatggata acctcctgtg aatgtgaccc tgcgatctct 107940 atccctatgg aatcaggtga atttataacg gatttagtga gcattgacgc tacctaattt 108000 ctagaggcca ggacttaatt gttctggcct ttcgtgtata ctatgagaaa taaaataaac 108060 gaattacttt atgaggaagc attacaattc cccataaata gattcactaa atcagatgga 108120 tctattaata gaactaagct aaaacaactt catccagact tccagcaaga cgcacttaat 108180 cttattttta ttaggagagc tgtagaagca catggtaagt tctttgggta tgagagagtt 108240 aagtatagaa ctatgcaaca gcaggttgag atctattgcc cagatcacga tggttattac 108300 tggcaaacgg ctagatctca cttagaaggg catggttgcc gattatgtgc acataaagtt 108360 gtgacacgct taacagaata tggagtgtac accgtaccag cacctttcca caaatttgta 108420 gttgacggtg agcacataat ttggtataat aaagatcaaa agttaaaaca ggagttaaag 108480 aaagatggag tacgtagtct ggtcaaaccc cttagctcta ctctcccaca aactgttcaa 108540 gacgacgtgg ggaaggcatg agataactgt taatgagaat ccacttaatc atcaacatcc 108600 tttagtccca attctctaca agattactat aaatggtaca gaatacctat tagatgattt 108660 cggtaaagag tttgtacaag aagccataga cgacaataac gtctctatgg gcgaattaat 108720 taatagagca tctaattttg ataagagttg gatgttaatt tggagtaact aatggaaatt 108780 gtagcaagcg tcttagtagt tatgttattc atagtaatac tcgtatcttt cgttattatg 108840 cgtagagcca gagaattagc agaggatcta acaaagttac gtggagaggt ggctaatctc 108900 aaacaacagc gtgaagctct gaagttattc gtggcccaag gtagcctaga acatacagca 108960 gaagacttca tagtatatct taaacgttac atgggaatca aataatggaa acattacttc 109020 ttgtactact aggcggctca ctcttaacta ttctaggctt gataattata tgcagtcgtc 109080 ttaatgttgt taagaatcta gaattaaata gtgagaatga aattcttaac cgagaaatta 109140 aacaacataa tatcgcagct cgtaagctgt tggataaact ggagaataga taatgtttac 109200 acaacaaacc attaaagtag ttgcagatca tgaactcgac acatgggatt acagctttaa 109260 agatctgact gctacagtac agtttaaatc tatgcaactg tcgtttttgc acattgatct 109320 tcaggcttgc aaagagtttc aggccaaact catgaatgct cgtaagatct ttggcacagc 109380 tcgcatcccg gcagataatc tgattaattc actaatcgat gctggataca aactcgtgaa 109440 gactgaggtg aaccgcccga gtactccagt gactattaga gatccgtggc agtcgggacc 109500 aatcgcaatg ctgaataact ccgataaaag attaatgtcg gatatgcaca atgttccgtg 109560 tggtggcgtg gtaaataaca cacaatcata taagtttggg gaagattgca gtccttctgt 109620 acaattgaaa catactcaag aagctataga gtctgacatg cgaagcgtag caccttctga 109680 tgctggagtt tctaggaact catatactat tcatctgagc acgccatcta gtaagaatga 109740 gagctttgct gatgcagtta tggaagctct tgttgatctg actaatagta agggttttaa 109800 ttaatggaac tagttactga atttactata ggccactgca aggtggcttt tctcaatgaa 109860 aagtctttta ataacgaaga aactattggg atctttgaaa ttaaagagga tgagtatgaa 109920 cgtgtggaaa ggctagcgta tttattcttt gctgatggtc atttaatgaa tgatctcaaa 109980 gagctagtat ttagactgcc aaaagatata cctttcacag ctagaaacat gtctcatatt 110040 gtcgctcaac taaatctaat atggattaaa ttagatcccg ctatagatag agtcataacg 110100 ttaaaagaaa gaccagttaa aaaatttaca ggtgatcaag cctgtgatat ctagtagtaa 110160 agcacaagcc tctacggagg ctttaaaata aaaagcaggt catctgacct taaaaaggag 110220 atataaataa tgtataactt taaagataaa ctaagacaga atgctggtaa aacaaaacct 110280 aaggagaaca ataatgttcc taatgctaaa gtacttccat ttcctgaact aactcaagaa 110340 gaaaaagata aactagcatt tatgttcgat gcacttatag gattaagtgg acgactgtac 110400 tccactctta gtattaatga gaaacgctta gtagagatgt tctgcaaacg ttatccagtt 110460 acttcaaagg gtaatgcaca taaagaaggt gaaaaagcat tcacagtctt aaaaatcagt 110520 gcatatgcac atcgtttctt agtttatgga ttacagatgg aatacttcac catcccacca 110580 tttaagtcat tccatatctt tgaagatgaa cgaatgaata ttgatgatct ttatggagat 110640 acgtacaaat attcaggaag tccattccat gatgcattaa ggagctaccc tactagttac 110700 ctaccagtta gagcatcaca aggtaaagct agcatctttg ctatatactc aggatctaaa 110760 gcatctgatc tagagactta tatatttaaa gagcgtaaat ctgtgcttcc agatgaagca 110820 ttatataata atccccttct tccagaaaaa gaaccagaga aaattgatgt aactccacca 110880 atcccatctg agtctcaata tttaatctat gagaaacaag ttaagttatc tctagacatt 110940 gggttagtta caccgcttgc tgcagcattc atcaatagat atctagaagt tcctgacgag 111000 ttccacacac gttataacac gaatattcag atcacagatg agttaattgc acttgcaaaa 111060 gatacaccta ctaacactga ggcgtaccaa aaagcatacc aagagttact gtaagatcct 111120 gcatgtctcg gtgcacaata cctgtaccga gactcaaata caaactctcg ttgacagata 111180 cttaaatgta tgttatcata actctatgag ttaatagaat taaccattta gagttgccac 111240 acagaaggtg tggtggttca ggaatcttaa attactgact tgtagcgtag gttacggagc 111300 gacaggacaa gtcaacaggt aatttaataa agattactag aaacatcacg agaccttctg 111360 gcgggggcaa agctctatat gagaaccctg gggggatctt atatttaaaa taaaagtaaa 111420 acttttgtac acataattta cattcaagat gatacactcg gttgccgctc atctgctagg 111480 atggcacaac actttctagt agactcctaa gtttctagga aaactctcgt caactcaggc 111540 ggttttcgtg ttcctagaaa ctctagcaat aggcggttta atacagggcg gtttaataca 111600 ggcgttt 111607

Claims (9)

살모넬라균에 특이적인 사멸능을 갖는, 서열번호 1로 표시되는 염기서열로 이루어지며, 수탁번호 KFCC11708P인, 박테리오파지 SH9.
Bacteriophage SH9 having the nucleotide sequence shown in SEQ ID NO: 1 and having accession number KFCC11708P, which has a killing activity specific to Salmonella.
삭제delete 제1항에 있어서, 상기 살모넬라균은 살모넬라 하다(Salmonella Hadar) 또는 살모넬라 티피뮤리움(Salmonella Typhimurium)인, 박테리오파지 SH9.
The method according to claim 1, wherein the Salmonella is Salmonella Hadar or Salmonella Typhimurium bacteriophage SH9.
제1항의 박테리오파지 SH9을 포함하는, 항생용 조성물.
An antibiotic composition comprising the bacteriophage SH9 of claim 1.
제1항의 박테리오파지 SH9을 포함하는, 사료 첨가용 조성물.
A composition for feed addition comprising the bacteriophage SH9 of claim 1.
제5항의 사료 첨가용 조성물을 포함하는, 사료.
A feed comprising the feed additive composition of claim 5.
제1항의 박테리오파지 SH9을 포함하는, 소독제.
A disinfectant comprising bacteriophage SH9 of claim 1.
제1항의 박테리오파지 SH9을 포함하는, 세척제.
A cleansing agent comprising the bacteriophage SH9 of claim 1.
제1항의 박테리오파지 SH9을 인간을 제외한 개체에 투여하는 단계를 포함하는, 살모넬라균에 의한 감염성 질병의 예방 또는 치료 방법.A method for preventing or treating an infectious disease caused by Salmonella, comprising the step of administering the bacteriophage SH9 of claim 1 to an individual other than a human.
KR1020170176343A 2016-12-26 2017-12-20 Novel Salmonella specific bacteriophage SH9 and antibacterial composition comprising the same KR102003759B1 (en)

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Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2007, Vol. 73, No. 14, pp. 4543-4549.
International Journal of Food Microbiology. 2014, Vol. 172, pp. 92-101.*

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