KR101996315B1

KR101996315B1 - Composition comprising extracts of natural substance for alleviating itch or atopic dermatitis

Info

Publication number: KR101996315B1
Application number: KR1020160032536A
Authority: KR
Inventors: 성민규; 전선재; 최원석; 정영필
Original assignee: (주)셀아이콘랩
Priority date: 2016-03-18
Filing date: 2016-03-18
Publication date: 2019-07-04
Also published as: KR20170108506A

Abstract

Disclosed is a cosmetic composition for alleviating itching and improving atopic dermatitis containing natural product extract as an active ingredient and a process for producing the same. The cosmetic composition according to the present invention is an extract of natural products, which comprises a herbal extract, a white pelvis extract, a golden extract and a Rhodiola extract. According to the present invention, it is possible to relieve skin itching caused by suppression of an immune activator without side effects and to improve atopic dermatitis have.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for alleviating itching and improving atopic dermatitis comprising an extract of natural products as an active ingredient,

The present invention relates to a cosmetic composition containing natural product extract as an active ingredient and exhibiting an effect of alleviating itching and atopic dermatitis, and a method for producing the same.

Atopic dermatitis is a chronic and recurrent inflammatory skin disease that causes abnormalities in the stratum corneum, which is the protective barrier of the skin. Symptoms include red rash, sour skin, dry skin, thickening, and scurvy (dandruff skin bark). The area that develops is the inside of the joint, and it looks good on the back of the elbow, the back of the knee, and the neck. These atopic dermatitis occur in 3-5% of children under 5 years of age, and up to 10% at most. In addition to the genetic factors, environmental factors have also recently been on the rise, and the number of patients suffering from atopic dermatitis is increasing in adolescents and adults as well as in infancy or childhood.

Itching refers to skin conditions that cause discomfort, such as light scratching, temperature changes, stress, or chemical, physical, or electrical stimuli. It may also be accompanied by symptoms of skin disorders such as atopic and contact dermatitis, and also of susceptible skin holders.

Skin diseases and itching caused by external allergens such as atopic dermatitis, psoriasis and contact dermatitis are caused by specific immune cells in the body such as T cells and macrophages, which activate TNF-a and various cytokines and histamine (Br J Haematol, 1996; Cancer Biother, 1995).

Although topical and general medicines such as steroids, antihistamines, immunosuppressants, etc. have been used as therapies for treating such itching and atopic dermatitis, it has been known that oral administration causes drowsiness, lowered immunity, , The stability of the composition for external application for skin is poor, and the use thereof is limited. Therefore, there is a continuing need for new substances that have less side effects on the human body and have a stimulation-relieving effect.

Korean Patent Laid-Open No. 10-2012-0100414

It is an object of the present invention to provide a cosmetic composition which has high skin stability by using a natural extract and exhibits an itching relieving effect due to the suppression of immunological activity factors and a method for producing the same.

It is another object of the present invention to provide a cosmetic composition which has high skin stability and suppression of an immunological activity factor and which shows atopic dermatitis improving effect using a natural product extract and a method for producing the same.

The above object is achieved by a cosmetic composition for alleviating itching and atopic dermatitis according to the present invention, which comprises as an active ingredient, an extract of Rhododendron sulphate, a white line extract, a golden extract and a Rhodiola extract.

Preferably, the above-mentioned Hwasun extract, Hwangbuk extract, Golden extract and Rhodiola extract are obtained by dipping a sample of Hwasungcho, Hwasun, Hwangwi, Golden and Wolyun into an extraction solvent, extracting hot water at a predetermined temperature and pressure and freeze- Can be provided.

Preferably, the Hwangsu extract, the white line bark extract, the golden extract and the Rhodiola extract may be extracted with an extraction solvent of 5 to 15 times by weight of each sample.

Preferably, the extract of Rhododendron japonica extract, Rhodiola extract, Golden extract and Rhodiola extract is 0.20 to 0.40% by weight, 0.10 to 0.30% by weight, 0.015 to 0.025% by weight and 0.005 to 0.015% by weight based on the total weight of the cosmetic composition, %. &Lt; / RTI >

Preferably, the cosmetic composition may further comprise beta-1,3 / 1,6-glucan.

Preferably, the beta-1,3 / 1,6-glucan may be included in an amount of 0.02 to 0.03% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition further comprises glycerin, propylene glycol, and sodium hyaluronate, wherein the glycerin, propylene glycol, and sodium hyaluronate are present in an amount of 8 to 11 wt% , From 8 to 11% by weight and from 0.3 to 0.6% by weight.

According to the present invention, the above objects can be accomplished by the method of the present invention, which comprises the steps of: Extracting each of the above samples with hot water to prepare a herbal extract, a white pelvis extract, a golden extract and a Rhodiola extract; And a method for preparing a cosmetic composition for alleviating itching and atopic dermatitis, which comprises the step of mixing the herbal extract, white line extract, golden extract and Rhodiola extract.

Preferably, the step of preparing the extract may further include a step of lyophilizing the herbal extract, the white pelvis extract, the golden extract and the Rhodiola extract, which are extracted from the hot-water extract, into a powder form.

Preferably, in the step of mixing the extracts, the extracts of Rhododendron japonica extract, Rhodophyta japonica extract, Golden extract and Rhodiola extract are contained in an amount of 0.20 to 0.40% by weight, 0.10 to 0.30% by weight, 0.015 to 0.30% 0.025% by weight and 0.005% by weight to 0.015% by weight.

The cosmetic composition according to the present invention exerts the effect of alleviating the itching caused by suppression of the immunologically active factor by using a natural extract.

In addition, the cosmetic composition according to the present invention exerts the effect of improving the atopic dermatitis due to the suppression of the immune activating factor by using the natural extract.

Hereinafter, the present invention will be described in detail.

In one aspect, the present invention provides a cosmetic composition for alleviating itching and improving atopic dermatitis, comprising as an active ingredient, an extract of Rhododendron japonica, a white line extract, a golden extract and a Rhodiola extract.

In another aspect, the present invention provides a method for preparing a sample, comprising the steps of: Extracting each of the above samples with hot water to prepare a herbal extract, a white pelvis extract, a golden extract and a Rhodiola extract; And a method for preparing a cosmetic composition for alleviating itching and improving atopic dermatitis, which comprises mixing the perennial herb extract, white line extract, golden extract and Rhodiola extract. At this time, the method may further include a step of lyophilizing the herbal extract, the white pelvis extract, the golden extract and the Rhodiola extract prepared by extracting hot water to prepare a powder.

As used herein, the term "active ingredient" refers to a component that can exhibit the desired activity alone, such as a carrier that exhibits the desired activity alone or is itself inactive.

Houttuynia cordata Thunberg is a perennial herb that belongs to Saururus chinensis. In the summer and autumn, we use the outposts in the root. It is used for pneumonia, acute chronic bronchitis, intestinal infections, urinary tract infections, boils, and when you can not see urine because you have excellent fever and drainage. Appearance has vertical wrinkles on the trunk, the nodes are clear, the lower nodes have thin roots, and the vagina is tender and prone to bending. Leaves are dried or shrunk, but when opened they are heart-shaped. Leaves are pointed at the ends, with dark yellowish green or yellowish brown on the top, and gray or gray brown on the underside.

It refers to the root bark of the white ring (Dictamnus dasycarpus Turcz.), Which has excellent antipyretic and fungal inhibitory action, and is effective for skin rash and nervous dermatitis due to heat. It grows in the low hills of the foot of the various places, and white or pink flowers bloom in the summer, and the root is caught in autumn, washed with water, peeled and dried. (Dictamines) of 0.02 to 0.03% in the root shell and 0.13% of alkaloids in the roots of the roots of the plants. Rice, saponin, campesterol, sitosterol, and essential oils. In the private sector, the seeds are used to cough and throat in Qatar while the seeds are used as a herbal medicine for the outpost. The outposts have a therapeutic effect on athlete's foot, but fresh outposts cause dermatitis.

Gold (Scutellariae Radix) is a physiologically active and pharmacologic action in a herb that exhibits fever and fever, dilates the blood vessels, further acts to lower blood pressure, and inhibits atherosclerosis. Baikkalin, a kind of flavonoids contained in gold, acts as a sedative and acts as a hemostatic agent because it reduces the permeability of capillaries. Baikalin is hydrolyzed into baicalein and glucuronic acid, baicalen is diuretic, and glucuronic acid is detoxifying.

Coptidis (Rhizoma) is the root of the grasshopper grasses of Mt. Yellow roots are said to be one after another, and it is called "Hwangryeong". The main ingredients are alkaloids and saponins, and the main component of alkaloids is berberine. Berberine has a blood pressure lowering action, has a strong action, antipyretic, antimicrobial, sedative. It has also been reported that there are no side effects.

The extract in the present invention means not only a crude extract but also all kinds of processed products of the extract, for example, additional processing such as drying, concentration, fractionation and fermentation.

In one illustrative embodiment, the perennial herb extract, white line extract, golden extract and Rhodiola extract are used in an amount of 5 to 15 times, or 8 to 12 times, more preferably 10 to 12 times, Is preferable in terms of improving efficiency and economical efficiency in manufacturing, alleviating itching, and improving the effect of improving atopic dermatitis.

In one illustrative embodiment, the perennial herb extract, the white line bark extract, the golden extract and the chrysanthemum extract may be obtained by extracting water with an extraction solvent.

In one illustrative embodiment, the perennial herb extract, the white pelvis extract, the golden extract and the peony extract may be contained in an amount of 0.1 to 1.0% by weight based on the total weight of the cosmetic composition on a dry weight basis. In order to improve the efficiency and economical efficiency of the preparation, the itching relief and the improvement of atopic dermatitis, it is preferable that the concentration of the herbal composition is 0.2 to 0.9 wt% based on the total weight of the cosmetic composition, , Or 0.3 to 0.8 wt%, or 0.4 to 0.7 wt%, or 0.5 to 0.6 wt%.

In one exemplary embodiment, the perennial herb extract, the pale yellow extract, the golden extract, and the peony extract are contained in an amount of 0.20 to 0.40 wt%, 0.10 to 0.30 wt%, 0.015 to 0.025 wt%, respectively, based on the total weight of the cosmetic composition, More preferably from 0.005 to 0.015% by weight, more specifically from 0.25 to 0.35% by weight, from 0.15 to 0.25% by weight, from 0.015 to 0.025% by weight and from 0.005 to 0.015% by weight, respectively. Accordingly, it is possible to effectively improve the effect of alleviating the itch of the natural product extract and improving the atopic dermatitis.

In one illustrative embodiment, the perennial herb extract, the white line extract, the golden extract and the chrysanthemum extract may be mixed in a weight ratio of 30: 20: 2: 1 on a dry weight basis.

In an exemplary embodiment, the composition further comprises beta-1,3 / 1,6-glucan to provide excellent skin itching relief and atopic dermatitis-improving effects. Specifically, the beta-1,3 / 1,6-glucan may be included in an amount of 0.02 to 0.03% by weight based on the total weight of the cosmetic composition.

The beta-glucan is a polysaccharide containing glucose as a structural component and is composed mainly of a polymer of glucose containing two or more β-1-2, 1-3, 1-4, 1-6-glucopyranose bonds . Beta-1,3 / 1,6-glucan has a number of 1,6 linkages in the beta-1,3-glucan backbone, a chain of glucose molecules with 6 side chains connected at positions 1 and 3 it means. Beta-1,3 / 1,6-glucan can be obtained from the cell wall of baker's yeast ( Saccharomyces cerevisiae ) and is also known to be contained in various species of mushrooms such as algae and manure, mushrooms and mushrooms.

In an exemplary embodiment, the composition further comprises glycerin having the formula C ₃ H ₅ (OH) ₃ , propylene glycol having the formula C ₃ H ₈ O ₂ , and sodium hyaluronate being the sodium salt of hyaluronic acid Thereby providing excellent skin itching relief and atopic dermatitis improvement effect.

In an exemplary embodiment, the glycerin, propylene glycol, and sodium hyaluronate may be included in amounts of 8 to 11 wt%, 8 to 11 wt%, and 0.3 to 0.6 wt%, respectively, based on the total weight of the cosmetic composition.

The cosmetic composition disclosed in the present invention contains, as an active ingredient, a mixture of four natural extracts at an appropriate ratio, and thus, compared to a composition containing each single extract, the immunological activity factor , Synergy effect, reduction of skin irritation, improvement of itching and improvement of atopic dermatitis. In addition, this effect is very economical because it shows remarkable relief of itching and improvement of atopic dermatitis with only four natural extracts without addition of additional natural extracts.

In the present invention, the formulation of the cosmetic composition is not particularly limited and can be appropriately selected according to the purpose. For example, it may be formulated into a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition But is not limited to.

In addition, the cosmetic composition may further contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, without impairing the main effect. The cosmetic composition may further include a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected and used by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof is 0.01 to 5% by weight, or 0.01 to 3% .

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Examples.

The extracts of Hwangseongcho, Hwangseongi, Hwangbyeon, Golden, and Hwangryn were prepared by extracting hot water and hot water at high temperature and high pressure in 1 L of water per 100 g. Then, each composition was prepared at the blending ratio shown in Table 1 below. At this time, when hot water is extracted, the high temperature and high pressure preferably have a range of 100 to 115 ° C and 1.3 to 1.8 kgf / cm ² , respectively.

[Table 1]

Test Example One.

In this test example, it was confirmed that the composition of Example 1 inhibits the production of IL-1β and TNF-α, which are inflammatory cytokines induced by LPS in mast cells.

Specifically, HMC-1 cells were added to a 24-well microtiter plate containing DMEM (Dulbecco's Modified Eagle's Media) medium containing 5% FBS at a concentration of 2 × 10 ⁵ per well, And cultured to 80% growth. Then, each composition obtained from Table 1 was added to serum-free DMEM medium at a concentration of 20 μg / ml and cultured for 24 hours. Then, the cell culture medium was collected, centrifuged, and the supernatant was harvested. The amount of TNF-α and IL-1β secreted in the cell culture medium was measured using ELISA kit (Mouse ELISA set, BD Bioscience, San Diego, USA).

For this, anti-mouse TNF-α and IL-1β were dispensed into the microplate as a capture antibody and coated overnight at 4 ° C. This was washed with PBST (0.01 M PBS, pH 7.2, 0.05% Tween 20) and blocked with 10% FBS solution. After washing with PBST, the cell culture supernatant was dispensed into each microplate and allowed to react at room temperature for 2 hours. After washing with PBST, diluted biotinylated antimouse TNF-α detection antibody and streptavidin-hos radial peroxidase conjugate were added and reacted at room temperature for 1 hour. In the case of IL-1β, biotinylated anti-mouse IL-1β detection antibody was added and reacted for 1 hour. Streptavidin-horseradish peroxidase conjugate was added and reacted for 30 minutes. Thereafter, it was washed with PBST again, and phosphate citrate buffer (pH 5.0) supplemented with OPD (o-phenylenediamine) and H ₂ O ₂ was added thereto, followed by dark reaction at room temperature for 30 minutes. After completion of the reaction with 2N H ₂ SO ₄ , the absorbance was measured at 490 nm using a microplate reader.

As a result, as shown in Table 2, it was confirmed that the composition of Example 1 significantly inhibited the production of IL-1? And TNF- ?.

[Table 2]

Test Example 2.

In this test example, it was confirmed that the composition of Example 1 inhibits the activity of caspase-1, an enzyme that activates IL-1β in macrophages and keratinocytes.

Specifically, the activity of caspase-1 was tested according to the manufacturer's protocol using caspase-1 colorimetric assay kit (R & D system). Cell culture and composition treatment were carried out in the same manner as in Test Example 1 above, and further, Caspase-1 inhibitor (Ac-YVAS-CMK) was used to compare. First, HMC-1 cells were lysed and incubated with the same amount of caspase-1 substrate for 2 hours, and the absorbance was measured at 405 nm.

As a result, as shown in Table 3, the natural composition of Example 1 significantly reduced activation of caspase-1, an enzyme that activates IL-1β in macrophages and keratinocytes, and Caspase-1 Inhibitor (Ac-YVAS-CMK) and was shown to be involved in the caspase-1 mediated inflammatory mechanism.

[Table 3]

Test Example 3.

In this test example, it was confirmed that the composition of Example 1 treated with DNCB and TNF-α inhibited the expression of TARC (thymus and activation-regulated chemokine) in inflammatory keratinocytes.

For human keratinocyte cell line, HaCaT cells by using a DMEM culture medium and adjusted to 5 × 10 ⁵ seeded in 12-well plates 18 hours before the culture. Thereafter, the medium was removed, and each of the compositions obtained from Table 1 was treated with DNCB and TNF-α to induce inflammation. After 24 hours, the supernatant was harvested and centrifuged (12,000 rpm, 3 minutes) A sample was obtained. TARC content was quantified and measured using a TARC immunoassay kit (R & D, Germany).

As a result, as shown in Table 4, it was confirmed that the composition of Example 1 remarkably suppressed the expression of TARC.

[Table 4]

Test Example 4.

The composition of the cosmetic composition was prepared according to the composition of Table 5 below and used in the following test. Beta-1,3 / 1,6-glucan was supplied by Korea Research Institute of Bioscience and was used with a purity of 93.3%.

[Table 5]

As atopic patients, 20 or more patients suffering from atopic dermatitis for more than 2 years were randomly selected to test atopic dermatitis improvement and itching relieving effect on the cosmetic compositions of Preparation Examples 1 and 2. Specifically, after washing, the cosmetic compositions of Preparation Examples 1 and 2 were applied to the affected area for 2 weeks, twice a day for 4 weeks, and then the degree of itching and degree of atopic dermatitis were determined by visual examination and visual inspection. Itching, and atopic dermatitis were evaluated in three stages: marked effect, efficacy, and no difference.

As a result, as shown in Table 6, the cosmetic composition of Preparation Example 1 containing four natural extracts showed excellent atopic dermatitis improvement and itching relief, whereas the cosmetic composition of Production Example 2 showed very slight effect, Was found to be very effective in improving atopic dermatitis and alleviating itch.

[Table 6]

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims

The present invention relates to a cosmetic composition comprising, as an active ingredient,
The above-mentioned Hwasun extract, Huangshi bark extract, golden extract and Huanghui extract are obtained by immersing each sample of Hwasungcho, Hwangbyeol, Golden, and Huangsil in an extraction solvent and extracting hot water at a predetermined temperature and pressure,
In the process of mixing the above-mentioned Houttuynia cordata extract, white roots extract, golden extract and Rhodiola extract extracted from each of the above-mentioned Sophora edulis, Tetranychus cordatus, Golden and Yellow roots, Wherein the cosmetic composition comprises 0.20 to 0.40% by weight, 0.10 to 0.30% by weight, 0.015 to 0.025% by weight and 0.005 to 0.015% by weight of the cosmetic composition, respectively, based on the total weight of the cosmetic composition.

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The method according to claim 1,
The cosmetic composition for improving the itching relief and atopic dermatitis according to any one of claims 1 to 5, wherein the perennial herb extract, white line extract, golden extract and Rhodiola extract are extracted with an extraction solvent of 5 to 15 times by weight of each sample.

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The method according to claim 1,
Beta-1,3 / 1,6-glucan. &Lt; / RTI >

6. The method of claim 5,
Wherein the beta -1,3 / 1,6-glucan is contained in an amount of 0.02 to 0.03% by weight based on the total weight of the cosmetic composition.

6. The method of claim 5,
Glycerin, propylene glycol, and sodium hyaluronate,
Wherein said glycerin, propylene glycol and sodium hyaluronate are contained in an amount of 8 to 11% by weight, 8 to 11% by weight and 0.3 to 0.6% by weight based on the total weight of the cosmetic composition, A cosmetic composition for improving dermatitis.

Immersing each of the samples of Rhizoctonia sp.
Extracting each of the above samples with hot water to prepare a herbal extract, a white pelvis extract, a golden extract and a Rhodiola extract; And
And a step of mixing the herring root extract, white line extract, golden extract and Rhodiola extract,
In the step of mixing the extracts, the extracts of Rhododendron japonica extract, Rhodophyta extract, Golden extract and Rhodiola extract are respectively added in an amount of 0.20 to 0.40% by weight, 0.10 to 0.30% by weight, 0.015 to 0.025% by weight, And 0.005 to 0.015% by weight, based on the total weight of the cosmetic composition.

9. The method of claim 8,
The method according to claim 1, further comprising the step of lyophilizing and drying the herbal extract extracted from the hot-water extract, the white pelvis extract, the golden extract and the rhododendron extract to a powdery state, thereby improving the symptoms of itching and atopic dermatitis &Lt; / RTI >

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