KR101985431B1 - Ionic liquid compositions and cosmetic compositions containing the ionic liquid compositions - Google Patents

Abstract

The present invention relates to an ionic liquid composition and a cosmetic composition comprising the same, and more particularly to a natural extract, wherein the natural extract is injected with an anion and has anti-inflammatory activity.

Description

TECHNICAL FIELD The present invention relates to an ionic liquid composition and a cosmetic composition containing the ionic liquid composition and a cosmetic composition containing the ionic liquid composition.

TECHNICAL FIELD The present invention relates to an ionic liquid composition and a cosmetic composition containing the same, and more particularly, to an ionic liquid composition having an excellent anti-inflammatory activity and having an ionic effect and a cosmetic composition containing the same.

In the universe including the earth, far infrared rays, ions, magnetic waves, high frequency, low frequency, static electricity, classical electric wave and microwave are present as electromagnetic wave energy. When such electromagnetic energy is present at a high density, qualitative and quantitative metering is easy. However, if the energy is low or very low density, qualitative and quantitative metering becomes very difficult. On the other hand, it is recognized that human mind, consciousness, and potential ability exist as wave energy in the area where quantitative measurement is difficult in modern science, that is beyond the analysis limit of modern analytical science.

In this natural world, there exists very low energy (weak energy), that is, quantitatively and precisely unknown energy form. They are also functioning in space, in matter, in the universe including the Earth, and in the spirit world, such as human consciousness, unconsciousness. Recently, the unknown energy of this weak state has been bundled and defined as subtle energy.

On the other hand, nuclear energy in nuclear fusion and fusion, and superconducting world in pursuit of electric resistance zero, which are treated as energy problems at the district level, are high energy fields compared with weak energy.

In contrast, the recent development of "the world of Ki", "acupuncture world of oriental medicine", "the world of low-temperature infrared far infrared rays", "positive electrostatic potential" or " World "is a world of weak energy.

In recent years, from the viewpoint of dealing with such weak energy, attention has been focused on the nucleus and in particular, the electrons circling the nucleus. Negative ions composed of single electrons, anions having more electrons and cations lacking electrons There is a great deal of interest in the effects on the environment, the environment and the health of life. The field in which this electron participates is the world of anion or cation, and in particular, it is scientifically verified in the atmosphere of the environment, that is, the environment of the living body, and much research has been done on the life and environment.

According to Atmospheric Electrochemistry, in the natural space centering on the atmosphere, there exist ionic particles (fine particles) combined with several molecules having positive (+) and negative (-) small charges have. A positive charge is defined as a cation, and a negative charge is defined as an anion. These ionic microparticles are caused by mutual action, and the phenomenon of various phenomena such as generation of charge, neutralization, migration and attachment to other particles are constantly generated in the atmosphere.

In addition, the atmosphere has a very small amount of electric conductivity, and a weak conduction current flows in the vertical direction by the atmospheric electric field, which is referred to as an open-circuit current. The negative charge (negative charge) on the surface is neutralized or lost by this conduction current, but it is constantly ionized and ionized by the radioactive material that constantly radiates to the cosmic ray from the universe and the earth surface have. Due to these ions, the conductivity of the atmosphere increases with altitude without deteriorating away from the surface. These atmospheric electric fields fluctuate according to the weather change of each season every day.

The conductive layer (conductive layer) in the upper atmosphere is known to maintain a potential estimated at about 300 kV with respect to the earth. The phenomenon that replenishes the known current of the atmosphere is the development of the lightning cloud.

On the other hand, when we look at the magnetism field of the earth, the intensity distribution varies depending on the position and the height, and it is one of the important factors for ion generation in the earth's atmosphere. This is called the earth's geomagnetic effect. The Earth's magnetic field, which varies depending on the latitude and longitude of the earth, is closely related to atmospheric electric fields.

The above factors are the basic factors of ionization and ion generation in the upper atmosphere. The atmospheric ion currents produced by these have a profound effect on the atmospheric space of the earth's life (ie, the whole of the ecosystem).

The fresh air of nature is helping to grow plants and animals together with heat and light energy of the sun by the power to raise our life. However, the fact that ions are present in air and these ions greatly affect the physiology of our bodies is not well known compared to other elements of the natural world. In the past, battery therapy, which treats the mind and body in search of clean air and water, has been popular, but its importance is not so generalized. However, modern people 's awareness of ion effect is to protect the health from water pollution, air pollution and food pollution. Introduction of water purifier and air purifier, forest bath, half-bath, aromatherapy (treatment using fragrance or essential oil), and so on.

On the other hand, ions are generated by another physical phenomenon in the lower atmosphere. That is, it is a physical phenomenon called the Leonard effect. When a large water droplet is divided into several small water droplets, the surrounding air particles are charged with negative ions. In other words, water droplets are charged positively (+) and ambient air is negatively charged (-), which is called anionization of air. This phenomenon is called the Leonard Effect because it was discovered by Dr. Leonard, a Nobel physics winner. This is evidenced by the freshness around the waterfall in the nature and around the fountain in the downtown city.

On the other hand, even when the plant performs transpiration, the divergent water vapor particles are charged positively (+) and ambient air is negatively charged (-). Because of this principle, when plants are grown indoors, negative ions are increased, and fine dust particles, odor particles and harmful particles which are positively charged in the room are neutralized to clean room air will be.

In addition, apart from the natural world, a technique of artificially charging the surrounding air with negative (-) has been developed, and a corona discharge (discharge) method is a typical method. This is because a high voltage of several thousands to several tens of thousands of volts is applied between a positive electrode plate and a negative electrode plate which are kept at a constant distance and air is discharged while discharging them, It is the way to get. Many negative ion air cleaners on the market adopt this method. However, there are two or three problems to be overcome in the above corona discharge method. A typical problem is that when the air is corona discharge, oxygen is accompanied by a small amount of ozone (O ₃ ) and nitrogen oxide (NO _x ). In addition, ozone (O ₃ ) generated together oxidizes the electrode plate to shorten the lifetime of the electrode and lower the production efficiency of the anion. Therefore, these problems must be solved.

Unlike the corona discharge, an electron emission type (electron emission type) utilizing only a cathode has been developed to solve the drawback of the corona discharge. The principle of such an electron emission type is that electrons are emitted from the cathode to negatively charge the surrounding air, so that ozone (O ₃ ) and nitrogen oxide (NO _x ) are not generated. There are other ways to generate negative ions by using natural minerals such as Tourmalline (tourmalline, also known as tourmaline in the city), clay fine particles and charcoal.

Drinking clear air in the natural waterfalls and forests, enjoying the forest bath is, on the other hand, drinking plenty of anion-rich air. To clean the indoor air with anion air cleaner in the home of a serious metropolitan city with air pollution, it is also intended to convert the positive atmospheric environment into the negative ion environment and to promote health.

In general, the anion-dominant environment stabilizes the mind of a person, and is known to enhance the natural healing power by enhancing the respiratory function and activating the whole body physiological function. This action is the same as increasing the life activities of animals and plants, and activates the entire natural ecosystem.

Blood and body fluids of the body are composed of electrolytes. The cation is composed of a hydrogen ion, a potassium ion, a sodium ion, a calcium ion, and an anion such as a chloride ion, a carbonate ion, a phosphate ion and a sulfate ion, and maintains a balance of a cation and anion.

The pH of a healthy person's blood is about alkaline at around 7.4, and the blood of a person with a chronic disease is generally more acidic than the blood of a normal person. As another example, body fatigue is caused by the accumulation of lactic acid in the muscle tissue, while the pH of the blood can be seen to be acidic due to the lactic acid produced. Thus, the acidification of blood can be expressed as the "physiological environment of cationic advantage." It is known that anion is added to activate the metabolism, and the action of the autonomic nerve is normalized to restore the health of the body.

However, most of the above-mentioned techniques are mostly effected by the effect of ions applied to the air and the air ion generating device.

Accordingly, the present inventors have made efforts to apply the effect of the anion to the natural extract in order to further enhance the anti-inflammatory activity of the natural extract, and as a result, the present invention has been completed.

KR 10-2013-0008823 A

Accordingly, an object of the present invention is to provide an ionic liquid composition excellent in anti-inflammatory activity.

Another object of the present invention is to provide a cosmetic composition comprising such an ionic liquid composition.

The ionic liquid composition according to the present invention comprises a natural extract, wherein the natural extract is in an anion-injected state and has anti-inflammatory activity.

The natural extract is characterized by being an extract of burdock, warshon, punggye, green tea, parsley, sesame leaf, and mackerel.

And the anion is injected through an electron emission method.

The cosmetic composition of the present invention is characterized by containing the aforementioned ionic liquid composition in an amount of 5 to 50% by weight based on the total weight of the cosmetic composition.

The ionic liquid composition and cosmetic composition containing the ionic liquid according to the present invention are not only superior in anti-inflammatory activity but also have a positive effect on the human body through ionic effect.

1 and 2 are graphs showing the results (cytotoxicity) of Test Example 1 of the present invention.
3 to 8 are graphs showing the results (anti-inflammation: NO production inhibitory effect) of Test Example 2 of the present invention.
9 to 14 are graphs showing the results of Test Example 3 of the present invention (anti-inflammatory activity: TNF-α production inhibitory effect).
15 is a graph showing the results (ion concentration) of Test Example 4 of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is characterized by a natural extract, wherein the natural extract is an ionic liquid composition in which an anion is implanted. In addition, such an anion-injected natural extract has an advantage of having an excellent anti-inflammatory activity as compared with a conventional natural extract.

That is, the present invention provides an ionic liquid composition by injecting an anion into various natural extracts in the past, and such an ionic liquid composition has an advantage that various anions can be given to the human body as described above.

Herein, the natural extract is not limited in its kind, but is preferably an extract of natural materials such as burdock, persimmon, crab, green tea, parsley, sesame leaf, and mackerel.

Here, the method for extracting the above extract may be any method that is usually extracted by an extraction method well known in the art, but it may suffice if it is extracted in a liquid state for injecting an anion.

A method of preparing such an extract will be described below as an example.

The natural extract is an extract which is soluble in a solvent. The type of the solvent is not limited, and any solvent acceptable in the art may be used, and water or an organic solvent may be used. The organic solvent may be at least one kind of alcohol having 1 to 5 carbon atoms including methanol, ethanol, propanol, isopropanol, and butanol. It is possible to use at least one of organic solvents in combination, but there is no need to be limited thereto.

The extraction method may be selected from the group consisting of a hot water extraction method, a cold extraction method, a reflux cooling extraction method, a solvent extraction method, a steam distillation method, and an ultrasonic extraction method, but is not limited thereto.

As an example of the extraction method, at least one extraction solvent selected from alcohols having 1 to 5 carbon atoms, which is 1 to 50 times by volume of the dry weight thereof, is added by drying and pulverizing the gravel material, Stirring the mixture for a day, extracting the active ingredient, and then filtering it.

As another example, it is also possible to prepare a granular material by drying and pulverizing, adding 1 to 50 parts by volume of the dry weight thereof, extracting the active ingredient at 70 to 90 ° C for 10 to 20 hours, .

At this time, the natural material can be dried by a natural drying method such as a shade, and the pulverization particle size may be sufficient to be easily extracted, for example, about 10 to 100 mesh. Filtration may be carried out according to a generally known method. If necessary, it may be concentrated and used. Alternatively, it may be dried and mixed with a solvent.

In addition, the natural materials such as burdock, persimmon, tarragon, green tea, parsley, sesame leaf, and mulberry can be independently extracted and mixed with the extracts, , But does not limit it.

In the present invention, burdock, persimmon, crab root, green tea, parsley, sesame leaf, and mulberry are used as the natural materials because they have excellent antioxidative and anti-inflammatory activities as well as various pharmacological effects . In addition, there is no example of enhancing the pharmacological effect through the interaction of these seven natural substances.

In the present invention, the mixing ratio of the natural materials or the mixing ratio of the natural extracts is not limited. Preferably, 20 to 200 parts by weight of the green tea, 20 to 200 parts by weight of the green tea, 50 to 200 parts by weight of the green tea, 20 to 200 parts by weight of sesame leaves, 20 to 200 parts by weight of sesame leaves, and 20 to 200 parts by weight of seschi.

In addition, the present invention is most remarkable in that the above-mentioned natural extract is in an anion-injected state. The method of injecting such anion is as far as the conventional electron emission type (electron emission type) (-) electrons are released into the extract through the negative electrode and the anion is injected into the extract. Since the electron emission equation is sufficiently well known through the conventional techniques, detailed description thereof will be omitted.

That is, when the anion is injected into the extract, the extract becomes an ionic substance and has the advantage of exhibiting the effect of an anion when applied to various cosmetic compositions and the like. In addition, such an ionic substance, that is, an ionic liquid, has an advantage that it exhibits more excellent anti-inflammatory activity than conventional natural extracts.

Also, in addition to the above-described methods, anions in natural extracts can be injected through various methods known in the art.

Hereinafter, the present invention will be described in detail.

The burdock ( Arctium lappa L.) has been shown to have a beneficial effect on inflammatory diseases such as hepatitis as well as hypertension, gout and arteriosclerosis (10. Hwang & Kim, 2015). In addition, chlorogenic acid contained in burdock, arctigenin, one of the lignin species, and caffeoylquinic acid were found to be important components for the physiological activity of burdock (11 Chan et al., 2011).

Orostachys japonicus A. Berger has been used as a cancer treatment for a long time and pharmacological effects such as vasoconstriction, respiratory excitement, and intestinal intensification are known. The so far reported ingredients of taurine include triterpenoids such as friedelin, epi-friedianol, glutinone, and glutinol, and sterol-based substances such as β-sitosterol and campesterol. In addition, it contains various physiologically active ingredients such as flavoroids such as kaempferol and quercetin, aromatic acids such as 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid and gallic acid, and fatty acid esters (19 Choi et al. 2008). It has been reported that the saliva has a variety of physiological activities such as antioxidative, anti-inflammatory, and anti-cancer effects (20 Jung & Choe, 2016).

The Pueraria thunbergiana Bentham ) is a dry root of 칡, is used as a variety of medicinal herbs in Oriental medicine. The saponin components contained in the purpura showed hepatoprotective action, decreased alanine aminotransferase activity, and daidzein and puerarin, which are contained in Puerariae, have anti-inflammatory effects when given liver immunological damage (21Yun et al ., 2010).

The green tea is widely used all over the world, and it mainly manufactures various products such as functional foods, beverages and cosmetics by processing leaves. Particularly, green tea contains a polyphenol component catechin, (-) - epicatechin (EC), (-) - epigallocatechin (EGC), epicatechin-3-gallate (ECG), epigallocatechin- (EGCG). These polyphenols have been found to have excellent anti-inflammatory, anticancer, and whitening effects, and the green tea seed coat extract has a whitening effect and excellent anti-inflammatory effect et al., 2016).

The above-mentioned buttercups, especially Oenanthe javanica, is a medicinal plant which is highly utilized as a material and spice for functional food due to its unique flavor and pharmacological action. Isorhamnetin is a 3'-O-methylated metabolite of quercetin and has antioxidant, anti-inflammatory and anti-cancer properties (9 Won et al., 2016).

The perilla leaf (Perilla frutescens var. Japonica Herba) is known to contain a large amount of anthocyanin-based pigment and flavonoid-based ingredients, and hydrothermal extract or methanol extract has excellent anti-inflammatory, antiallergic and antioxidative effects 16. Kim et al., 2012).

The zoophyllum (Zan thoxylum piperitum DC) has long been used in Korea and China as a private and herbal medicine for digestive, gynecological, and anti-inflammatory drugs. It has been studied for its excellent antioxidant activity against physiological activity as well as whitening action by inhibition of tyrosinase, , Liver function protection and antimicrobial activity (17Xie et al., 2016).

On the other hand, the ionic liquid composition of the present invention as described above can be utilized in a wide range of fields, but is most preferably used as a cosmetic composition.

That is, the ionic liquid composition may be contained in an amount of 5 to 50% by weight based on the total weight of the cosmetic composition. In this case, the ionic liquid composition exhibits excellent anti-inflammatory activity while being safe against cytotoxicity as compared with cosmetic compositions containing conventional natural extracts .

In addition, the cosmetic composition of the present invention may contain any conventional ingredients other than the above-mentioned effective ingredients, including conventional additives such as diluents, excipients, buffers, dispersants, surfactants, colorants, perfumes or sweeteners can do. In addition, the cosmetic composition may further include a functional cosmetic ingredient, a moisturizer, and other additives that impart a cosmetic function to the skin, and may further include at least one cosmetically acceptable carrier incorporated in a general skin cosmetic composition . Examples of the carrier component include animal oils, vegetable oils, waxes, paraffin, silicone, lactose, silica, talc, water, ethanol, benzyl alcohol, glycerol aliphatic ester and aliphatic alcohol sulfuric acid. Of course.

In addition, the cosmetic composition according to the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, , And the like, but the present invention is not limited thereto.

In addition, the formulation example is the same as that of a conventional cosmetic composition, except that the ionic liquid composition of the present invention is added.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

( Example ): Preparation of sample

Thinning materials were mixed as shown in Table 1 below, and 10 parts by volume of water was added thereto. Then, the active ingredient was extracted at 80 DEG C for 10 hours and filtered to obtain each extract.

In addition, an ionic liquid composition was prepared by injecting an anion into the extract thus obtained by electron emission. At this time, the anion was charged while the positive electrode was shut off, and the anion was charged to the extract for 6 hours.

Hereinafter, each of the extracts is designated as HM 1, HM 2 and HM 3, and the ionic liquid compositions prepared by injecting the anions into HM 1, HM 2 and HM 3 are referred to as EHM 1, EHM 2 and EHM 3.

At this time, the green tea was shredded to 50mesh and shrunted to 50mesh, and the parsley was shredded to 50mesh by shade, and the sesame leaves were shredded to 50mesh, And the leaves were shredded in 50mesh.

Mixing ratio of natural materials Raw material HM 1 (% by weight) HM 2 (% by weight) HM 3 (% by weight) green tea 28 26 14.30 Parsley 18 8 14.28 Sesame 9 8 14.28 Chuppy 9 16 14.28 burdock 18 26 14.30 Welcome 9 8 14.28 Changwon 9 8 14.28

( Test Example  1): cytotoxicity

The toxicity of the sample was confirmed by the RAW 264.7 macrophage line. Cytotoxicity was measured using a ^{Cell Titer 96 ® AQueous One Solution Cell} Proliferation Assay Kit 5- (3-caroboxymeth-oxyphenyl) -2H-tetrazolium inner salt assay (MTS assay) using a.

Specifically, 100 μl of 1 × 10 ⁴ cells / ㎖ cells were dispensed into a 96-well plate and cultured in a 5% CO ₂ incubator at 37 ° C for 24 h. Then, the 96-well plate was transferred to a 96-well plate in a concentration of 80 μl, and each well was mixed with 20% CellTiter 96®AQueous One Solution Reagent and DMEM 80% containing 10% FBS And incubated at 37 ° C in a 5% CO ₂ incubator for 4 h, and the absorbance was measured at 490 nm.

The results are shown in Fig. 1 and Fig.

As can be seen from FIGS. 1 and 2, it was found that the samples had no significant cytotoxicity at concentrations below 50%, and those above 75% showed that the survival rate was lower than 60% in all samples.

From the results, it was confirmed that the ionic liquid composition according to the present invention did not increase cytotoxicity compared to general natural extracts, and was not significant at concentrations below 50%.

( Test Example  2): NO production inhibitory effect

From the macrophage cell line RAW 264.7 cells, NO ₂ , a stable NO oxide, was measured in the culture supernatant as an indicator of nitrogen monoxide production using the Griess Reagent System (Murakami et al., 2000). RAW 264.7 cells were suspended in DMEM containing 10% FBS, and 100 μl of 1 × 10 ⁴ cells / ml of cells were added to each well of a 96-well plate and cultured for 24 h at 37 ° C in a 5% CO ₂ incubator. Each sample was treated with 1 μl of each sample concentration, and 1 μl of LPS was added only to the bottom of the sample-treated plate, followed by culturing for 24 hours. After transferring 80 μl of the culture to a new 96-well plate, 40 μl of Substrate Buffer 1 was dispensed using Nitric Oxide Detection Kit, and 10 minutes, Substrate Buffer 2 was dispensed and waited for 20 minutes. Absorbance was measured at 540 nm.

The results are shown in FIGS. 3 to 8. FIG.

As can be seen from Figs. 3 to 8, HM 1 to 3 and EHM 1 to 3 all exhibited a concentration-dependent NO production inhibitory effect, but compared to HM 1 to 3, 10% higher than the others.

( Test Example  3): TNF -alpha formation inhibitory effect

RAW 264.7 cells were suspended at 1 × 10 ⁵ cells / well in 96 wells, and pretreated at various concentrations. After 30 minutes, the cells were stimulated with LPS (100 ng / ml) for 24 h. Next, cell supernatants were collected and TNF-a was performed by ELISA. Uncoated 96 well plates were mixed with 1X coating buffer and Capture Antibody in a one-to-one ratio and vortexed, and 100μl was injected into a 96-well plate and treated at 2-8 ° C for 24h. Capture antibody coating was taken out in a dark room for 40 min and washed three times with 1 × PBST. For blocking, 200 ㎕ of 1 × assay diluent was injected and treated for 1 h in a dark room. After washing 3 × with 1 × PBST, 100 μl of sample and standard were injected and treated in a dark room for 2 h. Then, washing was repeated 3 times with 1 × PBST and 100 μl of detection antibody was injected. And then treated for 1 h in a dark room at room temperature again. After washing three times with 1 × PBST, 100 μl of AV-HRP was injected, treated for 30 min in a dark room at room temperature, washed 4 times with 1 × PBST, and 100 μl of TMB substrate was sprayed at room temperature Absorbance was measured at 450 nm after 15 min in the dark room.

The results are shown in Figures 9-14.

9 to 14, all HMs 1 to 3 and EHMs 1 to 3 all exhibited an effect of inhibiting TNF-alpha production in a concentration-dependent manner, and EHMs 1 to 3, which are superior to HMs 1 to 3, alpha production inhibitory effect.

( Test Example  4): ionization analysis

The ionization analysis was performed using an ion chromatography (Dionex) instrument. All samples were filtered by a Millipore filter (HAWP 04700) with a pore size of 0.45 μm, and then passed through a vial for an autosampler. The filtrate from which the particulate matter was removed from the sample was used as an analyte of the water-soluble ion component.

Ion concentration analysis buffer sieve sampler for after taken out carefully room inside the watch to pinsen NO ₂ using the ultra-pure water to the leachate and the leaching for 30 minutes and then 60 ℃ incubation in an ultrasonic cleaner, and the filtrate Ion chromatography (Dionex社) Ion concentration was analyzed using the instrument. The ion concentration was calculated using NO ₂ 458 as a conversion factor.

The results are shown in Fig.

As can be seen from FIG. 15, EHMs 1 to 3 showed significantly higher ion concentrations than HMs 1 to 3.

As can be seen from the above experimental example, it can be concluded that the ionic liquid composition prepared according to the present invention is excellent in cytotoxicity, NO production inhibitory activity, TNF-alpha inhibitory activity, and ionization concentration.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (4)

Including natural extracts,
The natural extracts are extracts of burdock, persimmon, crab, green tea, parsley, sesame leaf,
The ionic liquid composition for a cosmetic composition, wherein the natural extract has an anion-implanted state and an anti-inflammatory activity.
delete The method according to claim 1,
Wherein the anion is injected through an electron spinning method.
A cosmetic composition characterized by containing the ionic liquid composition according to any one of claims 1 to 3 in an amount of 5 to 50% by weight based on the total weight of the cosmetic composition.

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