KR101943623B1 - 멜팅 피크 분석을 이용한 타겟 핵산서열의 정량 - Google Patents

멜팅 피크 분석을 이용한 타겟 핵산서열의 정량 Download PDF

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Publication number
KR101943623B1
KR101943623B1 KR1020157023027A KR20157023027A KR101943623B1 KR 101943623 B1 KR101943623 B1 KR 101943623B1 KR 1020157023027 A KR1020157023027 A KR 1020157023027A KR 20157023027 A KR20157023027 A KR 20157023027A KR 101943623 B1 KR101943623 B1 KR 101943623B1
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KR
South Korea
Prior art keywords
nucleic acid
acid sequence
target nucleic
melting peak
value
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KR1020157023027A
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English (en)
Korean (ko)
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KR20150109478A (ko
Inventor
천종윤
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주식회사 씨젠
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Publication of KR20150109478A publication Critical patent/KR20150109478A/ko
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Publication of KR101943623B1 publication Critical patent/KR101943623B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/165—Mathematical modelling, e.g. logarithm, ratio

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020157023027A 2013-03-13 2014-03-13 멜팅 피크 분석을 이용한 타겟 핵산서열의 정량 KR101943623B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020130026837 2013-03-13
KR20130026837 2013-03-13
PCT/KR2014/002116 WO2014142575A1 (en) 2013-03-13 2014-03-13 Quantification of target nucleic acid using melting peak analysis

Publications (2)

Publication Number Publication Date
KR20150109478A KR20150109478A (ko) 2015-10-01
KR101943623B1 true KR101943623B1 (ko) 2019-01-30

Family

ID=51537118

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157023027A KR101943623B1 (ko) 2013-03-13 2014-03-13 멜팅 피크 분석을 이용한 타겟 핵산서열의 정량

Country Status (5)

Country Link
US (1) US10106840B2 (ja)
EP (1) EP2971115B1 (ja)
JP (2) JP2016508733A (ja)
KR (1) KR101943623B1 (ja)
WO (1) WO2014142575A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11485998B2 (en) * 2015-12-15 2022-11-01 Seegene, Inc. Signal extraction for a target nucleic acid sequence
KR101785687B1 (ko) * 2016-07-20 2017-10-17 (주)다이오진 다중 증폭 이중 시그널 증폭에 의한 타겟 핵산 서열의 검출 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009042728A1 (en) 2007-09-24 2009-04-02 Allelogic Biosciences Corporation Detection and /or quantification of nucleic acids
CN102827939A (zh) * 2012-09-07 2012-12-19 徐君怡 转基因玉米bt11品系的环介导等温扩增引物、试剂盒及其应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
AU635105B2 (en) 1990-01-26 1993-03-11 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556751A (en) 1991-04-25 1996-09-17 Amoco Corporation Selective amplification system using Q-β replicase
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
EP0730662A4 (en) 1993-09-10 1999-11-24 Genevue Inc OPTICAL DETECTION OF THE POSITION OF OLIGONUCLEOTIDES ON LARGE DNA MOLECULES
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU726501B2 (en) 1996-06-04 2000-11-09 University Of Utah Research Foundation Monitoring hybridization during PCR
ATE232880T1 (de) 1996-11-18 2003-03-15 Takeshi Imanishi Neue nucleotidanaloga
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
EP2341058A3 (en) 1997-09-12 2011-11-23 Exiqon A/S Oligonucleotide Analogues
EP1190097A2 (en) 1999-06-22 2002-03-27 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
DK1715063T3 (da) 2000-03-29 2011-05-16 Lgc Ltd Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
EP1288314B1 (en) 2001-08-31 2006-08-09 The University of Utah Research Foundation Real-time gene quantification with internal standards
PT1856257E (pt) 2005-03-05 2012-01-02 Seegene Inc Processos utilizando um oligonucleótido de especificidade dual e oligonucleótido de especificidade dual utilizado
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
GB201010237D0 (en) * 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
MX2017015093A (es) 2011-01-11 2023-03-10 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR101569479B1 (ko) * 2011-03-29 2015-11-16 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009042728A1 (en) 2007-09-24 2009-04-02 Allelogic Biosciences Corporation Detection and /or quantification of nucleic acids
CN102827939A (zh) * 2012-09-07 2012-12-19 徐君怡 转基因玉米bt11品系的环介导等温扩增引物、试剂盒及其应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Peyrefitte, CN, et al., JOURNAL OF CLINICAL MICROBIOLOGY, Vol.46(11), pp.3653-3659. (2008. 11.)*

Also Published As

Publication number Publication date
WO2014142575A1 (en) 2014-09-18
EP2971115A1 (en) 2016-01-20
JP2018183153A (ja) 2018-11-22
EP2971115A4 (en) 2016-03-02
JP2016508733A (ja) 2016-03-24
US10106840B2 (en) 2018-10-23
US20160068892A1 (en) 2016-03-10
KR20150109478A (ko) 2015-10-01
JP6703564B2 (ja) 2020-06-03
EP2971115B1 (en) 2022-07-27

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