KR101936690B1 - Formation method of nano-emulsion using a electrospray process - Google Patents

Abstract

The present invention relates to a nanoemulsion comprising electrospinning a solution by electrospinning, a method for producing the same, and a nanoemulsion cosmetic composition prepared by the method.

Description

TECHNICAL FIELD The present invention relates to a method for producing a nano-emulsion using an electrospray method,

The disclosure herein relates to nanoemulsions and novel methods for their preparation.

The nanoemulsion means an emulsion having a nano-sized particle size. When the droplet of the emulsion is formed into a nano-size, it has an advantage that it has properties such as efficient delivery of an oil-soluble component, increase of percutaneous absorption of an active ingredient, and translucent appearance . Therefore, development of nanoemulsion formulations is required in the cosmetics industry.

As a conventional emulsion method for the production of nano emulsion, a phase phase emulsification method and a D phase emulsification method have been mainly used. These emulsification methods are applicable to a method of making a fine emulsion by making the interfacial tension low in interfacial chemistry do. Also, high pressure emulsification methods, such as high pressure emulsifiers, have also been used, which utilize emulsifying devices having a strong transfer force.

However, in the phase-phase temperature emulsification method, it is necessary to calculate the kind and content of the optimal emulsifier for the oil-soluble component to be used by using a specific calculation formula. Therefore, there are problems in using various oil-soluble components and emulsifiers. The D phase emulsification method is an emulsifying method in which a surfactant is added to a polyhydric alcohol aqueous solution to dilute the interfacial tension between the oil phase and water, and therefore, it is necessary to use alcohol in the production of the nanoemulsion. Therefore, there is a problem that the formulation is limited. In the case of the high pressure emulsification method using a MF (micro fluidizer), there is a disadvantage that a multi-step process is required to pass the first emulsified pre-emulsion liquid again through a high-pressure emulsifier and a liquid phase state required for proceeding the process. There is a problem in terms of raw material use and containment conditions that the form of dispersion is limited.

In addition, the nanoemulsion prepared by the above processes usually leads to Ostwal Ripening, creaming, and content separation over time due to thermodynamics, chemical kinetics instability, and the like , There is a limit to the connection to the product with stability.

Therefore, there is a need to develop a nano emulsion which has a nano-sized particle size and is stable and not restricted by a formulation and a manufacturing method thereof.

Korean Patent Publication No. 10-2009-0073368 (2009.07.03)

The present invention provides a nano emulsion, a novel method for producing the same, and a nano-emulsion cosmetic composition produced by the method.

In order to accomplish the above object, one aspect of the present invention provides a nanoemulsion comprising electrospraying a solution by electrospray and a method for producing the same.

Further, one aspect of the present invention provides a cosmetic composition containing the nanoemulsion prepared by the above-mentioned electrospray method.

The method for producing a nanoemulsion according to the present invention has the effect of improving the problems in the conventional nanoemulsion manufacturing method in which the content and the kind of components contained in the composition are restricted by using an electrospray method. Particularly, since the emulsion particle shape and emulsion type can be changed, it is possible to manufacture various formulations such as cream, lotion, lotion and the like while having the same function when it is made of cosmetics or the like, have.

In addition, the nanoemulsion according to the present invention and the cosmetic composition containing the nanoemulsion can provide excellent skin moisturizing effect because of excellent long-term content stability with respect to the change over time and high transdermal absorption rate with respect to the active ingredient.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view illustrating a process of forming an emulsion by an electrospinning method in a method of manufacturing a nano emulsion according to an aspect of the present invention. FIG.
FIG. 2 is a view showing the characteristics of a nano emulsion and a comparative example formed through electric spraying according to an aspect of the present invention.
FIG. 3 is a graph comparing emulsified particles of an emulsion formed through electrospray according to an aspect of the present invention with emulsified particles of a general emulsion. FIG.
FIG. 4 is a graph showing the emulsion particle size of a nano emulsion formed through electric spraying according to an aspect of the present invention.
FIG. 5 is a graph showing changes in properties according to temperatures of Examples and Comparative Examples according to one aspect of the present invention. FIG.

The term " nano emulsion " as used herein means an emulsion having a nano-sized particle size. In addition, the term " electrospray " is the broadest concept of a method of spraying a liquid in an electric field.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a nanoemulsion prepared by an electrospray method.

The nano emulsion according to one aspect of the present invention can be produced by an electrospinning method to provide a nano emulsion in which the diameter distribution of nanoparticles is dense and the particle surface is smooth.

Another aspect of the present invention provides a method for producing a nanoemulsion comprising electrospinning a solution by electrospinning.

Also, specifically, one aspect of the present invention is

(a) preparing an aqueous solution;

(b) preparing an oily solution; And

(c) a step of spraying the aqueous solution prepared in the step (a) to the oil phase solution prepared in step (b) to prepare a nano emulsion.

In one aspect of the present invention, the aqueous solution in step (a) may be prepared by mixing at least one member selected from the group consisting of purified water, butylene glycol, glycerin, and a polar solvent. In this case, May include at least one selected from the group consisting of acetone and polyhydric alcohol.

The polar solvent may be from 10 to 50% by volume based on the volume of the water. If the volume ratio is less than 10 vol%, the polarity of the prepared aqueous liquid is too low to form the nano emulsion by electric spraying. If it exceeds 50 vol%, the volatilization on the water during electrospinning is large, It is out of range.

In addition, in the step (a), the aqueous solution may be made into a transparent single phase by mixing. At this time, mixing can be performed at 30 to 60 ° C using a paddle mixer at 40 rpm for 15 minutes

Further, in one aspect of the present invention, the method for preparing the nanoemulsion may further comprise a functional ingredient in the preparation of the aqueous solution of step (a), wherein the functional ingredient is at least one selected from the group consisting of Niacinamide, Adenosine, (Albutin). ≪ / RTI >

In one aspect of the present invention, in the step (b), the oil phase solution may be prepared by mixing silicon, ester, functional organic ultraviolet ray, etc., and the mixing may be performed at 30 to 60 ° C using a paddle mixer And can be carried out at 40 rpm for 15 minutes.

According to an aspect of the present invention, in the step (c), the aqueous solution may be accumulated in an oil phase solution by electrospray. Specifically, electrospray can be achieved by electrostatically dispersing the supernatant solution by discharging the supernatant solution into a metal capillary by means of a syringe pump. In this case, an electric field exceeding the surface tension of the droplet itself is applied to the droplet having a weak viscosity, and the droplet is declustered and injected by the repulsive force between the charges accumulated on the surface of the droplet. In this process, the solvent molecules of the droplet sprayed in the atmosphere are removed by evaporation, and the droplet is transformed into nano-sized fine particle form. As a result, nano-sized droplets are deposited in the oily solution to form an emulsion based on nano-sized emulsion particles. As described above, in the present invention, since the surface portion of the droplet is collapsed by the electrostatic spraying instead of the interfacial chemical method, and the particles are de-clustered, the particles are deposited on the oil phase. It is possible to significantly reduce the constraint on the condition of < RTI ID = 0.0 > A schematic diagram of a schematic electrodeposition process is shown in Fig.

The humidity, temperature, radial voltage, distance between the needle and the collector (radiating distance), and the spinning speed can be adjusted to create an emulsion with homogeneous morphology and stable formulation properties of nano-emulsified particles during electrospray. Specifically, the electric spraying is carried out at a humidity of 30 to 40%, a temperature of 25 to 30 DEG C, an electric field radiation voltage of 0.5 to 1.5 KV / cm, a voltage of 10 to 15 kV, a radiation distance of 10 to 15 cm and a radiation velocity of 40 to 100 m / min . ≪ / RTI > At this time, it may be performed under an electric field of 0.5 to 1.5 KV / cm, preferably 1 KV / cm.

In one aspect of the present invention, the nano-emulsion prepared by electrospinning may have a particle size of 50-400 nm, and the particle distribution is very dense.

In one aspect of the present invention, the nano-emulsion prepared by electrospray may have a viscosity of 12000 cP or more, 12500 cP or 13000 cP or more, and a viscosity of 15500 cP or less, 15000 cP or less, 14500 cP or 14000 cP or less. Specifically, in one aspect of the present invention, the nano emulsion may have a viscosity of 12000 cP or more and 15,500 cP or less and 13000 cP or more and 15,000 cP or less.

In one aspect of the present invention, the nano-emulsion prepared by the electrospray method has less change in the point hardness than the nano-emulsion prepared by the conventional method even after the preparation time, have.

Another aspect of the present invention provides a cosmetic composition containing the nano-emulsion prepared by the above-mentioned electrospray method. Since the nanoemulsion according to the production method of the present invention has no limitations on the content and kind of constituents and has excellent formulation stability, the cosmetic composition can be provided in all formulations suitable for topical application.

For example, it can be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. In addition, the cosmetic composition according to the present invention may contain a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjusting agent, organic and inorganic pigments, a fragrance, have. The blending amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof can be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition have.

In addition, since the nanoemulsion contained in the cosmetic composition according to one aspect of the present invention is not only composed of nano-sized fine particles but also has a dense particle size distribution, it exhibits a high percutaneous absorption rate and moisture retention ability for an active ingredient, Effect can be provided.

Hereinafter, the present invention will be described with reference to the following examples and experimental examples. The Examples and Experimental Examples are intended to further illustrate the present invention and are not intended to limit the scope of the present invention.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the invention.

[Composition of Example 1-2 and Comparative Example 1-4]

The constituent components and the content ratios of Example 1-2 and Comparative Example 1-4 according to one aspect of the present invention are shown in Table 1 below.

division Display name Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Floating Squalane 2.00000 2.00000 2.00000 2.00000 2.00000 2.00000 Ethylhexyl methoxycinnamate (Ethylhexyl methoxycinnamate) 5.00000 5.00000 5.00000 5.00000 5.00000 5.00000 Dimethicone 10.00000 10.00000 10.00000 10.00000 10.00000 10.00000 Phenyl trimethicone 10.00000 10.00000 10.00000 10.00000 10.00000 10.00000 Caprylic / Capric triglyceride (Caprylic / Capric triglyceride) 10.00000 10.00000 10.00000 10.00000 10.00000 10.00000 Hydrogenated Polydecene (Hydrogenated Polydecene) 2.00000 2.00000 2.00000 2.00000 2.00000 2.00000 Methyl trimethicone 10.00000 10.00000 10.00000 10.00000 10.00000 10.00000 Cyclopentasiloxane < RTI ID = 0.0 > 10.00000 10.00000 10.00000 10.00000 10.00000 10.00000 Cetyl PEG / PPG-10/1 Dimethicone < RTI ID = 0.0 > 1.50000 1.50000 1.50000 1.50000 1.50000 1.50000 Sorbitan isostearate < RTI ID = 0.0 > 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Sorbitan sesquioleate (Sorbitan sesquioleate) 0.50000 0.50000 0.50000 0.50000 0.50000 0.50000 Ethylhexylglycerin 0.05000 0.05000 0.05000 0.05000 0.05000 0.05000 Phenoxyethanol 0.30000 0.30000 0.30000 0.30000 0.30000 0.30000 Water top water 34.60000 32.60000 34.60000 34.60000 32.60000 32.60000 Disodium EDTA
(Disodium EDTA) 0.05000 0.05000 0.05000 0.05000 0.05000 0.05000 Sodium chloride
(Sodium Chloride) 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000 Butylene glycol
(Butylene Glycol) 2.00000 2.00000 2.00000 2.00000 2.00000 2.00000 Niacinamide
(Niacinamide) - 2.00000 - - 2.00000 2.00000 Sum 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000

[Comparative Example 1 and Comparative Example 3] Preparation of general emulsion

The general emulsions were prepared according to the compositions of Comparative Examples 1 and 3 described in Table 1 above.

First, the water-based raw materials of Comparative Example 1 were mixed at 30 ° C to 60 ° C using a paddle mixer at 40 rpm for 15 minutes to prepare a transparent single-phase aqueous solution. Then, the oil phase material was mixed with the aqueous solution in the same condition to prepare an oil phase solution. The emulsion of Comparative Example 1 was prepared by adding the aqueous solution to the prepared oily portion, mixing and emulsifying the mixture with a homomixer for 15 minutes while stirring.

Also in Comparative Example 3, an emulsion was prepared in the same manner as in Comparative Example 1.

[Comparative Example 2 and Comparative Example 4] Preparation of nanoemulsion using MF (Micro fluidizer)

According to the compositions of Comparative Examples 2 and 4 described in Table 1, nanoemulsions were prepared using MF emulsification method.

First, the water-based raw material of Comparative Example 2 was mixed at 30 ° C to 60 ° C using a paddle mixer at 40 rpm for 15 minutes to prepare a transparent single phase aqueous solution. Then, the oil phase material was mixed with the aqueous solution in the same condition to prepare an oil phase solution. The prepared aqueous solution of Comparative Example 2 was added to the oil phase solution and primary emulsified by stirring with a homomixer for 15 minutes. The first emulsified product was cycled two times at 600-700 bar using a Micro fluidizer C10523 high pressure emulsifier (Microfluidics). Then, the nanoemulsion of Comparative Example 2 was prepared while cooling the temperature to room temperature.

In Comparative Example 4, a nano emulsion was prepared in the same manner as in Comparative Example 2.

 [Examples 1 and 2] Preparation of nanoemulsion by electrospinning

According to the compositions of Examples 1 and 2 described in Table 1, nanoemulsions were prepared using the production method of the present invention.

First, the water-based raw material of Example 1 was mixed at 30 ° C to 60 ° C using a paddle mixer at 40 rpm for 15 minutes to prepare a transparent single phase aqueous solution. Then, the oil phase material was mixed with the aqueous solution in the same condition to prepare an oil phase solution. Subsequently, the supernatant solution was injected into the syringe to carry out the electric injection, and the electric injection preliminary operation was performed so that the liquid phase could be positioned to the metal capillary through an automatic syringe pump .

Then, the aqueous solution was applied under the conditions of a humidity of 40%, a temperature of 25 to 30 DEG C, an electric field radiation voltage of 0.5 to 1.5 KV / cm, a radiation voltage of 10 to 15 kV, a radiation distance of 10 to 15 cm and an injection rate of 40 to 100 mu m / The nanoemulsion of Example 1 was prepared by electrospraying into the oil phase solution.

Also in Example 2, a nanoemulsion was prepared in the same manner as in Example 1.

[Experimental Example 1] Characteristic comparison according to the manufacturing method of emulsion

The outer appearance of the emulsion prepared in Example 1 and Comparative Examples 1 and 2 was photographed for comparison of properties and shown in FIG.

As shown in FIG. 2, it was predicted that the nano emulsion had a particle size of several nanometers in the case of Comparative Example 1, which was produced through a general emulsification process, in which the opaque white creamy phase was difficult to transmit. On the other hand, Example 1, which is a nano emulsion through electrospray, causes ray-like scattering with the wavelength region of incident light similar to Comparative Example 2, which is an emulsion produced through MF (Micro fluidizer) And a translucent liquid phase as a result of the partial transmission of light. Therefore, it can be predicted that Example 1 had a particle size of a nanometer size as in Comparative Example 2. It was found that nano-emulsion based on nano-sized emulsified particles was formed through electric injection.

[Experimental Example 2] Viscosity comparison and confirmation experiment according to the emulsion preparation method

For comparison of the viscosity of the emulsion prepared in Example 1 and Comparative Examples 1 and 2, the viscosity of the emulsion prepared in Example 1, Comparative Example 1 and Comparative Example 1 was measured using a Brookfield (DV-III programmable Rheometer) under the conditions of 64 spindles, 12 rpm, , And the viscosities of Comparative Examples 1 and 2 were measured, respectively, and the results are shown in Table 2.

Comparative Example 1 Comparative Example 2 Example 1 Viscosity immediately after manufacture (cP) 4,800 14,950 14,300 Viscosity after 7 days (cP) 4,230 10,800 12,800

From the results of Table 2, it can be seen that the emulsion prepared through general emulsification immediately after preparation (Comparative Example 1) had a viscosity of 4,000-5,000 cP, whereas Example 1 prepared by electrospray and Comparative Example 2 The emulsion had a viscosity of 14,000 to 15,000 cP which was 3 times higher than that of Comparative Example 1. [ This is because the emulsion particles present in the composition have a large number of particles through electrophoresis or MF emulsification. Thus, it was found that nano-emulsion based on nano-size emulsion particles was formed through the electric spraying.

Also, as a result of comparing the viscosity immediately after the preparation and the 7 days after the preparation, it was found that the variation of the viscoelasticity of the Example 1 produced by the electric spraying was two times or more smaller than that of the Comparative Example 2 using the MF. Therefore, it was confirmed that when the nano emulsion was prepared by electrospray, the viscosity descent phenomenon due to the emulsion aggregation was remarkably reduced.

[Experimental Example 3] Morphology determination and particle size analysis of nanoemulsion emulsified particles

The morphology of the nano-emulsified particles prepared in Comparative Example 1 and Example 1 was measured and compared using an optical microscope (manufacturer: Brookfield, product name: DV-III programmable Rheometer) and shown in FIG. The particle size of the emulsion of Example 1 was measured using a particle size analyzer (Zetasizer 3000HS from Malvern) and is shown in FIG.

As shown in FIG. 3, the nanoemulsion particles of Example 1 prepared by electrospinning had a much smaller diameter range than the particles of Comparative Example 1 prepared by the general emulsification method, and the surface states of the particles were smooth spherical I knew I had a status.

From FIG. 4, it can be confirmed that the diameter of the particles of Example 1 has a distribution of 50-500 nm, and the average particle diameter has a narrow particle distribution of about 270 nm.

[Experimental Example 4] Stability test of nanoemulsion

In order to compare the stability of formulations according to the preparation method, the emulsions of Comparative Examples 1 and 2 and Example 1 were stored at room temperature and high temperature (45 ° C) for 30 days, and the change of the emulsion properties was measured using an IXUS 850 IS camera 4) and is shown in Fig.

As shown in FIG. 5, in the general emulsion of Comparative Example 1, emulsion separation and aged deterioration with time were not observed in the change of 30 days, but Comparative Example 2, which was a nano emulsion produced by MF emulsification method, As a result, it lost its appearance with blue scattering light and changed into a milky white color with a low viscosity. It is believed that this is due to thermodynamics and chemical kinetics instability.

On the other hand, in Example 1, which was an emulsion prepared by electrospray, emulsion having a nano-sized particle size was stable under all conditions without external shape change and separation. This is because the particle size distribution of the emulsified particles pulverized by the applied electric field is more uniformly produced than that produced through MF emulsification, and the Ostwalt Ripening does not occur over time.

[Experimental Example 5] Skin moisturizing effect test of nano emulsion

The following experiment was conducted to confirm the skin moisturizing effect of Example 1 according to one aspect of the present invention.

First, the emulsions of Example 1 and Comparative Examples 1 and 2 were applied to the tops of seven volunteers who waited in the room under constant temperature and humidity conditions (20 to 22 ° C, relative humidity 40 to 60%) for 30 minutes before the start of the experiment. Respectively. At this time, skin moisture content was measured with Skin Surface Hygrometer (Skincon-200, Japan IBS Co., Ltd.) immediately before and immediately after application, and skin moisture loss was measured with TEWAMETER TM 210 (Courage & Khazaka, Germany). Thereafter, skin moisture content and skin moisture loss were measured after 1 day, 3 days, 7 days, and 14 days after application of the control site and each test substance application site, and the results are shown in Tables 3 and 4 . The measured TEWL is expressed in g / m ² / h.

Comparative Example 1 Comparative Example 2 Example 1 Before use 2.7 2.8 2.6 after use 13 33 42

Item TEWL before application After application TEWL ㅿ TEWL (g / m ² / hr) Comparative Example 1 15.29 14.05 -1.24 Comparative Example 2 15.61 10.28 -5.33 Example 1 15.46 10.04 -5.49

As can be seen from the results of Table 3, it was found that the water retention effect was superior to that of Comparative Example 2, which is the nano emulsion, and Comparative Example 1, Particularly, in the case of Example 1 produced through electron injection, it was found that the water retention ability was better than that of Comparative Example 2 produced by the MF emulsification method. This is because the particle size uniformity of the nano emulsion is better than that of the first embodiment.

From the results of Table 4, it was confirmed that the nanoemulsion of Example 1 according to the present invention had a smaller decrease in the amount of percutaneous water loss after application than the emulsions of Comparative Examples 1 and 2. This is because in Example 1, a similar liquid crystal phase on the lamellar layer due to the nanoemulsion in the composition is formed, so that it has moisture retaining ability from the skin when applying the skin.

Thus, it can be seen that the nanoemulsion of Example 1 according to the present invention exhibits a superior skin moisturizing effect.

[Experimental Example 6] Experiments of skin moisture permeability effect of nano emulsion

The transdermal absorption of the emulsions of Comparative Examples 3, 4 and Example 2 was measured in order to examine the transdermal absorption effect when the nanoemulsion contained niacinamide.

At this time, skin permeation test was performed three times using artificial skin (product name: EpidermFT, manufactured by MatTek). 48 占 퐇 of each sample was injected into the skin surface of 7.5 mm in diameter, and the amount of niacinamide permeated after 24 hours using a skin absorption measuring device (manufactured by Rapfine Co., Ltd., product name: Franz Cell) was analyzed by high performance liquid chromatography Respectively. The results are shown in the following Table 5 as the transmittance (%).

Comparative Example 3 Comparative Example 4 Example 2 Transmittance 3.57 13.6 15.7

As shown in Table 5, the niacinamide-containing nanoemulsion of Comparative Example 4 and Example 2 significantly accelerated the percutaneous absorption compared to Comparative Example 3. In particular, Example 2 produced by the electron diffraction method showed higher dermal absorption rate than Comparative Example 4 produced by the MF emulsification method. This is because, in the case of Example 2, it is composed of a dense nano-emulsion.

As described above, it was confirmed that the nano-emulsion produced by the electron-scattering method of the present invention has a dense particle distribution of nano-sized particles and is stable without separation under all conditions of determination of stability such as room temperature and high temperature. In addition, it was confirmed that the percutaneous absorption rate and the water holding ability were also excellent.

Claims (17)

delete (a) preparing an aqueous solution;
(b) preparing an oily solution; And
(c) preparing a nano-emulsion by electrospraying the aqueous solution prepared in the step (a) into the oily solution prepared in step (b);
A nano-emulsion prepared by an electrospinning process,
Wherein the nanoemulsion has a particle size of 50 to 400 nm and a viscosity of 12000 to 15500 cP. The cosmetic composition according to claim 2, wherein the aqueous solution of step (a) is prepared by mixing at least one member selected from the group consisting of purified water, butylene glycol, glycerin, and a polar solvent. The cosmetic composition according to claim 3, wherein the polar solvent comprises at least one selected from the group consisting of acetone and polyhydric alcohol. The cosmetic composition according to claim 3, wherein the polar solvent is 10 to 50% by volume based on the total volume of the water. 3. The cosmetic composition according to claim 2, wherein the oily solution of step (b) comprises at least one member selected from the group consisting of silicone, ester, and functional organic UV agent. 3. The cosmetic composition according to claim 2, wherein the aqueous solution further comprises at least one raw material selected from the group consisting of niacinamide, adenosine and arbutin. delete delete delete A method of making a cosmetic composition comprising a nanoemulsion,
(a) preparing an aqueous solution;
(b) preparing an oily solution; And
(c) preparing a nano-emulsion by electrospraying the aqueous solution prepared in the step (a) into the oily solution prepared in step (b);
Lt; / RTI >
Wherein the nanoemulsion has a particle size of 50 to 400 nm and a viscosity of 12000 to 15500 cP. 12. The method according to claim 11, wherein the droplets of the aqueous solution are changed into nano-sized particles by the electric spraying in the step (c). 13. The method of claim 12, wherein the shape variation of the droplet is by spraying the droplet by electrospinning and spraying, and by volatilizing in the atmosphere. 12. The method of claim 11, wherein the electrospray of step (c) is performed at a humidity of 30 to 40%, a temperature of 25 to 30 占 폚, a radiation voltage of 10 to 15 kV under an electric field of 0.5 to 1.5 KV / cm, And a spinning speed of 40 to 100 占 퐉 / min. delete A cosmetic composition characterized by being produced by the production method according to any one of claims 11 to 14. The cosmetic composition according to claim 2, wherein the composition is for skin moisturizing.

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