KR101929564B1 - Skin moisturizing cosmetic composition comprising polysaccharide extract of Phragmitis Rhizoma and serine encapsulated in nano-carrier system - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing which comprises, as an active ingredient, serine incorporated into a nongenol polysaccharide extract and a nanoparticle system. More specifically, the present invention relates to a cosmetic composition for skin moisturizing comprising 93 to 98% by weight of neutral sugar and 2 to 7% % Of urinary high polysaccharide extract comprising uronic acid, and serine incorporated in a nanoparticle delivery system.
The cosmetic composition of the present invention has no irritation to the skin and has excellent skin permeability of the moisturizing component by including serine incorporated into the nongenol polysaccharide extract and nanoparticle system as an active ingredient, And exhibits remarkably improved skin moisturizing effect.

Description

The present invention relates to a skin moisturizing cosmetic composition containing polysaccharide extract and a serine encapsulated in nano-carrier system,

The present invention relates to a cosmetic composition for skin moisturizing which comprises, as an active ingredient, serine incorporated into a nongenol polysaccharide extract and a nanoparticle system. More specifically, the present invention relates to a cosmetic composition for skin moisturizing comprising 93 to 98% by weight of neutral sugar and 2 to 7% % Of urinary high polysaccharide extract comprising uronic acid, and serine incorporated in a nanoparticle delivery system.

The skin is divided into three layers of epidermis, dermis and subcutaneous fat tissue in order from the outside, and functions to protect the human body from physical and chemical stimulation from external environment. In particular, skin carries about 65 ~ 70% of the human body's moisture, that is, various physiologically active substances necessary for the human body, and helps the skin to keep its soft and moist state. It plays a role. In addition, the epidermis is divided into stratum corneum, granular layer, superficial layer, and basal layer from the outside. Cells of the stratum corneum act like bricks, and intercellular lipids between keratinocytes act like mortar to form skin barrier (J. Invest. Dermatol. 80 (Suppl.), 44-49, 1983). The stratum corneum of the epidermis contains about 10 to 20% of water and exists in the outermost part of the human body, thereby inhibiting moisture evaporation to the outside of the body, while blocking excessive material penetration from the outside. These stratum corneum surfaces are wrapped with a thin natural protective film made of sebum from the sebaceous glands and perspiration from the sweat glands, which can prevent water evaporation.

The cells that constitute the stratum corneum have a high concentration of Natural Moisturizing Factor (NMF), which is a water-soluble component, which helps the skin to exhibit flexibility and maintain proper moisture. For example, substances such as amino acids (J. Invest. Dermatol. 54, 24-31, 1970). ≪ / RTI > However, there is a tendency that the skin temperature is controlled by the change of the environment or the life pattern, the artificial temperature control of the cooling / heating, the skin stress due to various stresses and environmental pollution generated in the social life, The moisture of the stratum corneum is decreased, the skin becomes dry, the surface becomes rough, and the skin appears to be dull and dull. Therefore, the need for a skin moisturizer is increasing.

Polyhydric alcohols, water-soluble polymers, organic acid salts and the like are generally used as moisturizers in cosmetics. Occlusive ingredients and water-absorbing properties that prevent oil from evaporating on the surface of the skin while being oil- (Humectant) is used to increase the moisture retention in the stratum corneum. Examples of the humectant include polyols such as glycerin, propylene glycol, 1,3-butylene glycol, and polyethylene glycol. As an occlusive ingredient, lipid components such as ceramides, essential fatty acids and lipid complexes are used.

However, they have a disadvantage in that the moisturizing effect is temporary and thus the skin moisturizing effect can not be maintained for a long time continuously or it is difficult to apply to various formulations.

Accordingly, the present inventors have developed a cosmetic composition that exhibits an optimal moisturizing effect through a combination of a moisturizing factor derived from a natural product and a natural moisturizing factor (NMF) incorporated in a nanoparticle delivery system, which exhibits no skin irritation and exhibits excellent moisturizing effect The present inventors have found that the high polysaccharide extract of reed roots exhibits an excellent moisturizing effect and the combination of serine incorporated in the nanoparticle delivery system can exhibit optimal moisturizing effect. It was completed.

Accordingly, an object of the present invention is to provide a cosmetic composition for skin moisturizing which comprises, as an active ingredient, a nongenol polysaccharide extract and a nanoparticle system containing serine.

In order to accomplish the object of the present invention, the present invention provides a cosmetic composition for moisturizing the skin, which comprises, as an active ingredient, a nongonopolysaccharide extract and a nanoparticle system containing serine.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for moisturizing the skin comprising, as an active ingredient, serine incorporated in a nongon polysaccharide extract and a nanoparticle delivery system.

In the present invention, the rachis is the root of a tall reed (Phragmites communis Trin.). The dried squid is flat, cylindrical, glossy on the surface and yellowish white. The node is comparatively hard and bright yellow. It has vertical wrinkles between the nodes. It is collected in late spring or early summer or autumn to remove the clay and mustache roots. Film) is peeled off, and it is used when it is fresh or it is dried by sunburn.

In the present invention, it is most desirable to obtain a high polysaccharide extract of Angelica keiskei koidz. The camel root extract of the present invention can be isolated using conventional solvents known in the art for extracting an extract from a natural product, that is, under ordinary temperature and pressure conditions, using a conventional solvent. In the present invention, the extracting solvent for obtaining the camel root extract may be a solvent used in a conventional extraction process, such as water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, Butylene glycol.

According to one embodiment of the present invention, the present inventors have studied an extraction method of various conditions in order to obtain an egg yolk polysaccharide extract showing excellent moisturizing effect. Specifically, the extraction yields were confirmed by various methods such as hot water extraction, alcohol extraction, and alkali extraction. As a result, it was confirmed that the method of hydrothermal extraction after alkali pretreatment of the sample showed the optimum extraction yield.

It is preferable that extraction of the brow by water is carried out by applying heat, and more preferably, the extract of the present invention is a hot-water extract of brow. The temperature of the water to be heated upon extraction with water is not limited to a specific range, but is preferably 60-100 ° C, more preferably 70-100 ° C, even more preferably 80-100 ° C, most preferably 90 -100 < / RTI >

The nasal polysaccharide extract according to the present invention is characterized in that it contains 93 to 98% by weight of neutral sugar and 2 to 7% by weight of uronic acid.

The uronic acid is characterized by being composed of galacturonic acid and glucuronic acid. In addition, the neutral polysaccharide may include rumoneose, arabinose, xylose, mannose, galactose, and glucose. The neutral polysaccharide may further include fucose, but is not limited thereto.

According to one embodiment of the present invention, as a result of the general component analysis of the camel root extract according to the present invention, at least 93% of the samples were contained as neutral sugars. Protein and KDO were not observed, and the content of acid was low. From the results of HPLC analysis, the low molecular weight material was removed by high performance size exclusion chromatography (HPSEC) It can be assumed that it is composed of polymer materials.

On the other hand, serine is the most important component of natural moisturizing factor (NMF). Amino acid is composed of 40% amino acid. Amino acid is composed of protein, which is usually obtained by hydrolyzing protein and amino group and carboxy group is one As bipolar ions attached to carbon, they are classified into acidic, basic, hydrophilic (polar), and hydrophobic (non-polar) depending on the nature of the side chain. About 20 species are present in measurable amounts as the major constituent of amino acids present in the human body (Kim, Hwang, & Tak, 2005), among which serine accounts for 14.7%. Serine is an indispensable amino acid and can be synthesized from other metabolites in the body. It absorbs moisture by the hydroxyl ions of serine to maintain moisturizing.

≪

The inventors of the present invention judged that the moisturizing effect of the skin would be more effectively increased when the serine, which is the most abundant NMF component in the skin, is supplied from the outside. However, serine is a water-soluble substance and has a disadvantage that it is not high in penetration into the skin. The serine represented by the above formula has a terminal carboxyl group, and such a functional group is ionized in an aqueous solution and has a property of being easily dissolved in water. These physical properties can be regarded as a fundamental reason for the difficulty of percutaneous absorption of serine. In order to promote the percutaneous absorption of serine, it is necessary to apply a technique that can induce to increase the usability and suppress the water solubility.

Generally, for effective skin penetration, hydrophobic substances are more effective than substances having hydrophilic properties. This is because the hydrophobic substance rather than the hydrophilic substance is more effective for intercellular lipid interaction to pass between the intercellular lipids including the ceramides distributed in the stratum corneum of the skin, Because

 To improve this, the present inventors have designed nanosransporters such as liposomes, solid lipid nanoparticles (SLN) and the like, and intend to increase skin moisturizing power by incorporating serine therein.

In the present invention, the nanoparticle delivery system can be used without limitation as long as it is known in the art, and includes a nano delivery system to be developed in the future. Advantageously, the nanoparticle delivery system comprises a liposome, a solid lipid nanoparticle, a micelle, a millisphere, a microsphere, a nanosphere, a millicapsule, a microcapsule, Nano-capsules, microemulsions, and nanoemulsions, and most preferably liposomes or solid lipid nanoparticles, but are not limited thereto.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

Preferably, the cosmetic composition of the present invention may be characterized by being a W / O cream, an O / W cream, an O / W essence and a hydrogel formulation, most preferably a hydrogel formulation, but is not limited thereto .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The present invention also provides a cosmetic composition characterized in that 0.2 to 2% by weight of the cornified polysaccharide extract and 2 to 8% by weight of serine incorporated in the nanoparticle delivery system are contained in relation to the total weight of the cosmetic composition.

The cosmetic composition comprising the lanolin-rich polysaccharide extract of the present invention and serine incorporated into the nano-delivery system as an active ingredient is superior to the cosmetic composition comprising the lanolin-rich polysaccharide extract or the serine incorporated into the nanoparticle system, And can be used as an excellent moisturizer without causing any skin troubles with respect to skin conditions such as sensitive skin and atopic skin by being made of a low-irritation raw material of a natural ingredient.

The cosmetic composition of the present invention has no irritation to the skin and has excellent skin permeability of the moisturizing component by including serine incorporated into the nongenol polysaccharide extract and nanoparticle system as an active ingredient, And exhibits remarkably improved skin moisturizing effect.

Fig. 1 shows the result of evaluating the yield of polysaccharide extraction of burrs according to the addition amount of 10N NaOH.
FIG. 2 is a graph showing the HPSEC chromatogram results of the egg yolk extract according to the alkali extraction conditions (1 ml of 10N NaOH, 2 ml of 10 N NaOH, 3 ml of 10 N NaOH, 3 ml of 10 N NaOH and dialysis)
Fig. 3 shows the results of evaluating the moisturizing effect of hydrogel containing liposomal serine over time (A: skin moisture content at each measurement time, and B: change in skin moisture content with time).
Fig. 4 shows the results of evaluating the moisturizing effect of the hydrogel containing solid lipid nanoparticle serine over time (A: skin moisture content at each measurement time, B: change in skin moisture content with time ).
Fig. 5 shows the result of evaluating the moisturizing effect of the hydrogel containing the cornified polysaccharide extract over time (A: content of skin moisture at each measurement time, and B: change in skin moisture content with time).
Fig. 6 shows the result of evaluating the moisturizing effect of the hydrogel containing the cornified polysaccharide extract and the solid lipid nanoparticle serine over time (A: change in skin moisture content with time, B: The graph showing the water content curve under the curve).

Hereinafter, the present invention will be described in detail.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

≪ Example 1 >

Preparation of Noogun and Polysaccharide Extracts

From the results of the previous studies for the development of the extraction process of crude ash, it was confirmed by various methods of hot water extraction, alcohol extraction and alkali extraction that the method of hot water extraction after alkaline adjustment pretreatment of samples showed the optimum extraction yield . Therefore, to adjust the pH of the samples before extraction, we tried to determine the optimum amount of NaOH added and the pH condition by observing the change of the extract yield of bean curd according to the addition amount of 10N NaOH. First, 10 g of Chinese brown roots were weighed and then immersed in distilled water (100 ml, w / v) 10 times as much as the weight. The alkaline solution was added with 10 N NaOH to 0, 1, 2, 3 and 5 ml, and the pH was measured at 2 ℃ for 2 hours. The alkaline and hot water extracts were neutralized with 1 M HCl, and then the supernatant was filtered and added twice with fermenting alcohol (95%). After incubation for 20 minutes, the supernatant was removed and only the precipitate was recovered. The recovered precipitate was washed with fermented alcohol (95%), dissolved again in 20 ml of distilled water, concentrated using a vacuum concentrator, and then lyophilized to measure the yield.

The results are shown in Table 1 and FIG.

The pH of the hot water extract was changed from pH 5.21 ± 0.06 before addition of NaOH to pH 7.98 ± 0.12 after addition of 1 ml to pH 12.76 ± 0.31 when 5 ml was added. Of the patients. The yield after freeze-drying of the extract was about 2.2% before 10N NaOH addition, but it was about 2.5% when 1ml was added, but about 5.7% when 3ml was added and about 7.3% when 5ml was added. It was confirmed that the yield difference was about 3 times or more. The results of this study showed that the extraction yield was increased when the pH was adjusted to 10 or higher before hot water extraction, but the difference between pH and extraction yield was significantly different between 3 and 5 ml of 10N NaOH I did not see it.

Therefore, considering the amount of NaOH added for pretreatment and the amount of HCl added for neutralization of pH adjustment after extraction, 3 ml of 10N NaOH was added to obtain the optimum yield.

≪ Example 2 >

Identification and Characterization of Physicochemical Properties of Extracts from Nogun Geopolysaccharide

In order to investigate the characteristics of key polysaccharides according to the extraction conditions, we investigated the changes of polysaccharide composition according to alkali extraction conditions. The crude extracts were prepared by adding 1N, 2N, and 3mL of 10N NaOH to the extracts. The extracts were concentrated and added with 3N NaOH (10N NaOH) and extracted for 24 hours (6 ~ 8kDa). The neutral sugar content of the sample was determined by the phenol-sulfuric acid method using galactose as the standard, the acid content was determined by the m-hydroxybiphenyl method using galacturonic acid as the standard, the content of TBA-positive material was 2-keto-3-deoxy -D-manno-octulosonic acid (KDO) as a standard and thiobarbituric acid method was used to modify the protein content of Bradford method using bovine serum albumin as a reference material. For the constitutional analysis, polysaccharide samples were hydrolyzed with 2 M trifluoro-acetic acid (TFA) at 121 ° C for 1.5 hours and converted to alditol acetate derivatives, respectively, and analyzed by GC. GC analysis was performed using GC ACME-6100 (Young-Lin Co. Ltd., Anyang, Korea) equipped with SP-2380 capillary column (0.2 μm film, 0.25 mm × 30 m, Supelco, Bellefonte, 220 ° C (12 min), 220 ° C to 250 ° C (8 ° C / min), and 250 ° C (15 min)) at standard temperature conditions of 60 ° C . The molar percentage of constituent sugars was calculated from the area ratio of the peaks, the reaction coefficient to the flame ionization detector (FID), and the molecular weight of alditol acetate derivatives of each constituent sugar. Meanwhile, the HPLC used for the purification was eluted with 0.5 M Ammonium formate buffer (pH 5.5) as a solvent at 0.5 mL / min using Agilent 1260 infinity equipped with Superdex 75 GL column and the standard curve obtained by measuring the sample was confirmed .

The results are shown in Table 2 below.

As shown in Table 2 above, as a result of the general component analysis of Angelicae Radix extract, 94% or more of all samples were contained as neutral sugars. Protein and KDO substances were not observed and the amount of acidic sugar was contained. The neutralized sugar content of the dialyzed sample after addition of 3 ml of 10N NaOH was lowest at 94.6%. As the amount of 10N NaOH was increased, the glucose content decreased and the content of arabinose and xylose increased significantly. In particular, the glucose content of the dialyzed samples after alkali extraction decreased to less than 50%, suggesting that arabinose had a structure of arabinoxylan of 11.6% and xylose of 27.6%.

On the other hand, as shown in FIG. 2, it was found that the low molecular weight substances were removed from the high-performance size exclusion chromatography (HPSEC) Could.

≪ Example 3 >

Manufacture of serine incorporated into nanofluidic systems

≪ 3-1 > Preparation of liposomized serine

The liposomal serine was prepared by partially changing the reverse phase evaporation method. 500 mg of phosphatidylcholine (PC) and 5 ml of ethanol were added to a round bottom flask, and ethanol was evaporated at room temperature for 40 minutes using a rotary evaporator. Ethyl ether (15 ml) was added thereto and stirred for 5 minutes to prepare an organic solvent phase in which PC was dissolved. On the other hand, 200 mg of serine was dissolved in 5 ml of tertiary distilled water to prepare the water phase. The organic solvent phase prepared above and the aqueous phase were mixed and stirred for 10 minutes to prepare a water phase aqueous emulsion. The water emulsion was added to the bath sonicator and homogenized by sonication at 20-25 ° C for 5 minutes. Then, the organic solvent phase was removed at 37 ° C using a rotary evaporator. Further, 1 ml of tertiary distilled water was added and the resulting organic solvent was completely removed by rotary evaporation for 15 minutes to obtain a liposomal serine dispersion. In order to control the particle size of the liposomal serine, the liposomal serine dispersion was sonicated at 37 ° C for 3 minutes and passed through a syringe filter having a pore size of 0.45 μm to prepare liposomal serine of 300 nm or less.

<3-2> Solid lipid nanoparticle formation  Manufacture of serine

Solid lipid nanoparticle serine was prepared by partially modifying the water emulsion method in water. After dissolving 500 mg of glycerol monostearate (GM) in a constant temperature water bath at 60 ° C, 200 mg of Span 80 was added and dissolved. 250 μl of an aqueous solution containing 50 mg of serine was added thereto and stirred at 1000 rpm for 40 minutes to obtain an oil-in-water emulsion. Then, 10 mg of Tween 20 was added and stirred for 40 minutes to obtain an aqueous water-in-oil emulsion. In order to solidify the warm water-based aqueous phase emulsion in a short time, cold water at 2-3 DEG C was added and stirred at 500 rpm for 30 minutes to prepare solid lipid nanoparticle serine. Finally, to adjust the particle size of solid lipid nanoparticulate serine, the solid lipid nanoparticulate serine was homogenized by repeating three cycles at room temperature and 500 bar pressure using a high pressure homogenizer to prepare solid lipid nanoparticles of 200 nm or less Fucerin was prepared.

<Example 4>

Evaluation of Moisturizing Ability of Hydrogel Type Cosmetic Composition

<4-1> Evaluation method of moisture

To measure the moisture content of skin, we used a Corneometer CM825 (Courage and Khazaka, Germany). This instrument measures the moisture content of skin by measuring the change in capacitance due to skin moisture. The moisture constituting the skin tissue shows a higher dielectric constant than the other constituents. Since the stratum corneum of the moisturizing state contains more moisture than the dry state, the dielectric constant of the skin is high, so that the electricity is easily accessible. Using this principle, the method of measuring the moisture of the skin measures the conductance as an electrostatic load capacitance, and the larger the measured value, the higher the moisture content.

Specifically, the water content of the skin was measured using a Corneometer CM825 (Courage and Khazaka, Germany) for 3 hours at intervals of 30 minutes after each sample was applied to the skin, and the change pattern of the skin moisture with time was measured as a function of time And the area under the moisture content change-time curves (AUMCC) was evaluated by evaluating the area under the graph and evaluating the moisturizing power of each sample.

<4-2> Hydrogel-based moisturizing cosmetic composition containing liposomized serine

A hydrogel-based moisturizing cosmetic base containing 0.5% by weight, 1% by weight or 1.5% by weight of liposomal serine with the composition shown in the following Table 3 was prepared and the moisture resistance was evaluated according to the method of Example <4-1> .

The results are shown in Table 4 and FIG.

As shown in Table 4 and FIG. 3, the skin moisturizing ability of the liposomized serine-containing hydrogel evaluated by the area under the moisture content change-time curve (AUMCC) But not to the concentration of serine. On the other hand, when 1% serine was contained in the evaluated concentrations, the best moisture was observed.

<4-3> Hydrogel-based moisturizing cosmetic composition containing serine in solid lipid nanoparticles

The solid lipid nanoparticles are loaded with serine in a solid lipid at a room temperature of nano unit size, and because of the nature of the lipid, the skin affinity and skin penetration ability can be increased, and thus the moisture of the serine along with the liposome can be increased This study was selected as the target system of this study.

A hydrogel-based moisturizing cosmetic base containing 1% by weight, 5% by weight and 10% by weight of solid lipid nanoparticles with the composition shown in the following Table 5 was prepared and its moisture resistance was measured by the method of Example <4-1> Respectively.

The results are shown in Fig.

As shown in Fig. 4, when the hydrogel base used in the evaluation of the moisturizing ability of the moisturizing agent containing solid lipid nanoparticles serine was changed to 1%, 5%, or 10% of solid lipid nanoparticles, The results showed that when the solid lipid nanoparticle serine was contained at 5%, the moisture content was the most excellent. Also, the solid lipid nanoparticle serine did not show the concentration-dependent increase in moisture as in liposomized serine. This concentration - independence was thought to inhibit the skin penetration of hydrophilic serine at higher concentration than liposome or solid lipid.

<4-4> Hydrogel-based moisturizing cosmetic composition containing oogeun polysaccharide extract

A hydrogel-based moisturizing cosmetic base containing 0.25% by weight, 0.5% by weight, 1% by weight or 1.5% by weight of Reed root extract (RRE = Extract of Phragmites communis Trinius) &Lt; 4-1 >.

The results are shown in Fig.

As shown in FIG. 5, when hydrogel-based moisturizing cosmetic formulations were prepared so as to contain 0.25%, 0.5%, 1%, and 1.5% of camellia sinensis extract, changes in skin moisture content were measured. Moisturizing cosmetics containing 0.25% Was evaluated as the best. This suggests that the structure of hydrogel is destroyed by the increase in the content of mugwort extract, which prevents the hydrogel from being able to exert its merit as a moisturizing agent of the hydrogel.

<4-5> A hydrogel-based cosmetic composition for moisturizing, containing a polysaccharide extract of Nogeun and a solid lipid nanoparticle serine

From the above experiments, it was observed that when the content of 0.25% is contained in the rape seed extract, 5% in the case of the serine loaded in the solid lipid nanoparticles, the skin moisturizing power is most effectively increased when contained in the hydrogel. Therefore, we tried to evaluate the skin moisture increase effect when they were combined.

Specifically, the addition of Reed root extract (RRE = Extract of Phragmites communis Trinius) to 0.25% and serine added to solid lipid nanoparticles to 3, 5, 7, 10% A gel-based moisturizing cosmetic base was prepared and its moisture resistance was evaluated according to the method of Example <4-1> above.

The results are shown in Fig.

As shown in Fig. 6, when serine loaded on the hydrogel-based polysaccharide extract and solid lipid nanoparticles was added to the hydrogel-based moisturizing cosmetic base, the moisturizing power was higher than that of the hydrogel-based polysaccharide extract alone. In particular, when it was added to hydrogel moisturizing cosmetic formulations with 0.25% of corn root polysaccharide extract and 3% of serine contained in solid lipid nanoparticles, it was evaluated to be the most moisturizing. This is probably due to the combination of moisture and polysaccharide extracts and serine in solid lipid nanoparticles, which have been absorbed by the skin and exerted a moisturizing effect.

The cosmetic composition of the present invention has no irritation to the skin and has excellent skin permeability of the moisturizing component by including serine incorporated into the nongenol polysaccharide extract and nanoparticle system as an active ingredient, The skin moisturizing effect is remarkably improved and the possibility of industrial use is high.

Claims (6)

A cosmetic composition for moisturizing the skin comprising, as an active ingredient, 0.2 to 2% by weight of a crude polysaccharide extract and 2 to 5% by weight of a nanoparticle system containing serine,
Wherein the candy polysaccharide extract comprises 93 to 98% by weight of neutral sugar and 2 to 7% by weight of uronic acid.
delete The cosmetic composition according to claim 1, wherein the neutral polysaccharide comprises rhamnose, arabinose, xylose, mannose, galactose and glucose.
The nanoparticle delivery system of claim 1, wherein the nanoparticle delivery system comprises a liposome, a solid lipid nanoparticle, a micelle, a millisphere, a microsphere, a nanosphere, a millicapsule, a microcapsule, Capsules, nano-capsules, microemulsions, and nanoemulsions.
The cosmetic composition according to claim 1, wherein the cosmetic composition is a W / O cream, an O / W cream, an O / W essence, and a hydrogel formulation.
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