KR101922741B1 - 생물학적 물질의 고도로 특이적인 포획 및 방출을 위한 방법 및 조성물 - Google Patents

생물학적 물질의 고도로 특이적인 포획 및 방출을 위한 방법 및 조성물 Download PDF

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KR101922741B1
KR101922741B1 KR1020137023006A KR20137023006A KR101922741B1 KR 101922741 B1 KR101922741 B1 KR 101922741B1 KR 1020137023006 A KR1020137023006 A KR 1020137023006A KR 20137023006 A KR20137023006 A KR 20137023006A KR 101922741 B1 KR101922741 B1 KR 101922741B1
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molecule
hydrogel composition
polyethylene glycol
biological material
alginic acid
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KR20140016896A (ko
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샤시 케이 머피
아담 해치
조지 한스만
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노스이스턴 유니버시티
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
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    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • GPHYSICS
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    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
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    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548Carbohydrates, e.g. dextran
    • GPHYSICS
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    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553Metal or metal coated
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01F25/42Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/431Straight mixing tubes with baffles or obstructions that do not cause substantial pressure drop; Baffles therefor
    • B01F25/4317Profiled elements, e.g. profiled blades, bars, pillars, columns or chevrons
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020137023006A 2011-02-03 2012-02-03 생물학적 물질의 고도로 특이적인 포획 및 방출을 위한 방법 및 조성물 Active KR101922741B1 (ko)

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US201161439166P 2011-02-03 2011-02-03
US61/439,166 2011-02-03
PCT/US2012/023859 WO2012106658A1 (en) 2011-02-03 2012-02-03 Methods and compositions for highly specific capture and release of biological materials

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KR20140016896A KR20140016896A (ko) 2014-02-10
KR101922741B1 true KR101922741B1 (ko) 2019-02-20

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US (1) US9927334B2 (https=)
EP (1) EP2670856B1 (https=)
JP (2) JP6120778B2 (https=)
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ES (1) ES2687144T3 (https=)
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Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014072465A1 (en) 2012-11-09 2014-05-15 Roche Diagnostics Gmbh In vitro capture and analysis of circulating tumor cells
EP2951585B1 (en) 2013-02-01 2018-01-03 The General Hospital Corporation Capture and release of particles from liquid samples
US20170074870A1 (en) 2014-03-14 2017-03-16 Northeastern University Microfluidic System and Method for Real-Time Measurement of Antibody-Antigen Binding and Analyte Detection
WO2015148512A1 (en) 2014-03-24 2015-10-01 Qt Holdings Corp Shaped articles including hydrogels and methods of manufacture and use thereof
US11583860B2 (en) * 2014-12-22 2023-02-21 Ecole Polytechnique Federale De Lausanne (Epfl) Microstructured thin hydrogel films
US9790467B2 (en) 2015-09-22 2017-10-17 Qt Holdings Corp Methods and compositions for activation or expansion of T lymphocytes
EP3378945B1 (en) * 2015-12-14 2021-04-28 Quanta Matrix Co., Ltd. Method for distinguishing particular cells from mixture of heterogeneous cells
CN109154614B (zh) * 2016-03-18 2022-01-28 四方控股公司 用于细胞分离的组合物、装置和方法
US10429387B2 (en) * 2016-07-27 2019-10-01 Universiteit Tweate Simple and affordable method for immuophenotyping using a microfluidic chip sample preparation with image cytometry
US11118163B2 (en) 2017-02-02 2021-09-14 Sartorius Stedim Biotech Gmbh Separation of cell populations by marker identification and sedimentation velocity
DE102017105195A1 (de) 2017-03-10 2018-09-13 Leibniz-Institut Für Polymerforschung Dresden E.V. Verfahren zur differenzierten Sequestrierung von Stoffen verschiedener Stoffgruppen mit Hilfe von sulfatierte oder sulfonierte Komponenten enthaltenden Hydrogelen
KR20190138646A (ko) * 2017-03-20 2019-12-13 큐티 홀딩스 코포레이션 면역 세포의 조절을 위한 방법 및 조성물
WO2019239359A2 (en) 2018-06-13 2019-12-19 University Of Washington Extruded hydrogel tubes and coaxial fibers and applications thereof
EP3626814B1 (en) * 2018-09-21 2023-03-08 Technische Universität Wien Production of cellular spheroids
JP7120547B2 (ja) * 2019-02-22 2022-08-17 国立研究開発法人理化学研究所 物質固定化剤、及び当該物質固定化剤を用いた物質固定化方法
US11976269B2 (en) * 2019-03-18 2024-05-07 Cellular Research, Inc. Precise delivery of components into fluids
US11668676B2 (en) * 2019-05-30 2023-06-06 The Regents Of The University Of California Microfluidic acoustic devices and methods
CN112708150B (zh) * 2020-12-08 2021-11-16 济南国科医工科技发展有限公司 用于循环肿瘤细胞的捕获及定点释放的水凝胶体系
WO2023034430A1 (en) 2021-09-01 2023-03-09 Bio-Techne Corporation Human vitronectin fragments and uses thereof
US11805995B1 (en) 2022-11-22 2023-11-07 King Faisal University Saliva collection kit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998012228A1 (en) 1996-09-19 1998-03-26 The Regents Of The University Of Michigan Polymers containing polysaccharides such as alginates or modified alginates

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2466116C (en) * 2001-11-07 2012-04-24 Eidgenossisch Technische Hochschule Zurich Synthetic matrix for controlled cell ingrowth and tissue regeneration
MXPA04006021A (es) * 2001-12-18 2005-08-19 Eth Zuerich Matrices de proteina modificada con factor de crecimiento para ingenieria de tejidos.
AU2003248273A1 (en) * 2002-07-12 2004-02-02 Mitsubishi Chemical Corporation Analytical chip, analytical chip unit, analyzing apparatus, method of analysis using the apparatus, and method of producing the analytical chip
CA2500392C (en) 2002-09-27 2012-11-27 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
EP2946666B1 (en) * 2004-04-30 2017-11-15 OrbusNeich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods of using same
SE0402476D0 (sv) * 2004-10-13 2004-10-13 Biacore Ab Preparation and use of a reactive solid support surface
CN101338036B (zh) 2007-07-06 2010-11-03 常州百瑞吉生物医药有限公司 生物相容快速凝胶化水凝胶及其喷雾剂的制备方法
JP2011505802A (ja) 2007-12-10 2011-03-03 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ パターニングされた細胞シート及びその製造方法
US20120077246A1 (en) * 2009-04-24 2012-03-29 The Board Of Trustees Of The University Of Illinoi Methods and Devices for Capturing Circulating Tumor Cells
US20140154703A1 (en) * 2011-01-06 2014-06-05 Alison Skelley Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998012228A1 (en) 1996-09-19 1998-03-26 The Regents Of The University Of Michigan Polymers containing polysaccharides such as alginates or modified alginates

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PLOUFFE, B.D 등, Lab on a Chip. 2009, 제9권 제1507-1510면.

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EP2670856A4 (en) 2014-12-03
US9927334B2 (en) 2018-03-27
ES2687144T3 (es) 2018-10-23
EP2670856A1 (en) 2013-12-11
JP2017077245A (ja) 2017-04-27
WO2012106658A1 (en) 2012-08-09
KR20140016896A (ko) 2014-02-10
EP2670856B1 (en) 2018-06-27
JP2014506671A (ja) 2014-03-17
SG10201600803SA (en) 2016-03-30
SG192587A1 (en) 2013-09-30
US20140057280A1 (en) 2014-02-27
JP6120778B2 (ja) 2017-04-26

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