KR101916453B1 - The chicken skewered food sauce composition using the lcatic acid bacteria cultures of jujube - Google Patents
The chicken skewered food sauce composition using the lcatic acid bacteria cultures of jujube Download PDFInfo
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- KR101916453B1 KR101916453B1 KR1020160170825A KR20160170825A KR101916453B1 KR 101916453 B1 KR101916453 B1 KR 101916453B1 KR 1020160170825 A KR1020160170825 A KR 1020160170825A KR 20160170825 A KR20160170825 A KR 20160170825A KR 101916453 B1 KR101916453 B1 KR 101916453B1
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- jujube
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- lactic acid
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Abstract
본 발명은 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에 관한 것으로서, 더욱 상세하게는 김치류 및 침채류로부터 분리 동정한 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-1612, 수탁번호 : KCTC13170BP)을 대추 추출물에 접종 및 발효시켜 제조된 유산균 발효 대추 추출물을 포함하는 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에 관한 것이다.
본 발명은 대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 유산균 발효 대추 추출물을 제조하는 유산균 발효 대추 추출물 제조단계(S100)와 상기 유산균 발효 대추 추출물에 물엿, 과당, 간장을 혼합하여 제1혼합물을 형성하는 제 1혼합단계(S200)와 상기 제 1혼합물에 토마토를 첨가하여 제 2혼합물을 형성하는 제 2혼합단계(S300)와 상기 제 2혼합물에 후추, 다진마늘, 생강을 혼합하여 제 3혼합물을 형성하는 제3혼합단계(S400)와 상기 제 3혼합물에 전분, 표고버섯 분말, 가라기난을 혼합하여 제 4혼합물을 형성하는 제4혼합단계(S500)와 상기 제 4혼합물을 60 내지 100℃로 가열하는 가열단계(S600)를 포함하는 것을 특징으로 한다.The present invention relates to a sauce composition for chicken skewers using fermented lactic acid bacteria of jujube and a method for preparing the same, and more particularly, to a lactic acid fermented product of lactic acid bacteria fermented with lactic acid bacteria Lactobacillus plantarum DUIJ-1612 KCTC13170BP) obtained by fermenting jujube fermented with fermented jujuba fermented in a fermented jujube fermented with fermented lactic acid bacteria.
The present invention relates to a fermented jujube extract preparation step (S100) for producing lactic acid bacteria-fermented jujube extract by inoculating and fermenting lactic acid bacteria Lactobacillus plantarum DUIJ-16112, KCTC13170BP to a jujube extract and a lactic acid bacteria fermented jujube extract, Fructose, and soy sauce to form a first mixture (S200); a second mixing step (S300) of adding a tomato to the first mixture to form a second mixture (S300); and a second mixing step A fourth mixing step S500 of mixing starch, mushroom powder and garaganin into the third mixture to form a fourth mixture, and a third mixing step S400 of mixing the minced garlic and ginger to form a third mixture, And a heating step (S600) of heating the fourth mixture to 60 to 100 占 폚.
Description
본 발명은 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에 관한 것으로서, 더욱 상세하게는 김치류 및 침채류로부터 분리 동정한 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-1612, 수탁번호 : KCTC13170BP)을 대추 추출물에 접종 및 발효시켜 제조된 유산균 발효 대추 추출물을 포함하는 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에 관한 것이다. The present invention relates to a sauce composition for a chicken skewer using a fermented product of lactic acid bacteria of jujube and a method for preparing the same, and more particularly, to a lactic acid fermented product of lactic acid bacteria Lactobacillus plantarum DUIJ-1612, accession no.: KCTC13170BP) in a jujube extract and fermenting the fermented jujube fermented jujube extract.
소스는 고대 로마시대부터 사용되어 온 것으로 주요된 것만 해도 400-500여종이 되며, 어원은 '소금을 기본으로 하는 조미용액'을 의미하는 라틴어의 'Salsa'에서 유래되었다. Sources have been used since ancient Roman times, and there are about 400-500 varieties of them, and the etymology is derived from Latin 'Salsa', meaning 'salt-based seasoning solution'.
소스는 서양요리에서 맛과 색상을 부여하여 식욕을 증진시키고 부재료의 첨가로 영양가를 높여 음식이 요리되는 동안 재료들이 서로 결합되게 하는 역할과 요리에 풍미를 더해주고 소화 작용을 도와주는 윤활유 역할, 요리의 맛과 외형수분을 돋우기 위해 소스의 중요성이 강조된다. The sauce adds flavor and color to the western dish to enhance appetite. It enhances the nutritional value by adding the sub ingredient. It helps the ingredients to be combined while cooking. It also serves as a lubricant that helps flavor and improves digestion. The importance of sauce is emphasized in order to enhance the taste and appearance of moisture.
또한 다양한 식재료의 이용으로 새로운 맛을 만들어 낼 수 있는데, 소스의 맛은 주 요리의 맛을 좌우한다고 할 수 있기 때문에 서양요리에서 큰 비중을 차지한다. In addition, the use of a variety of ingredients to create a new flavor, the taste of sauce, the taste of the main dish can be said to take up a large proportion of Western cuisine.
대한민국의 식품규격의 식품 공전상에 '소스류라 함은 동식물성의 원료에 향신료, 장류, 당류, 식염 및 식초 등을 가미하여 혼합한 것 또는 이를 숙성시킨 것으로서 식품의 조리 전화에 풍미 증진을 목적으로 사용되는 것을 말한다' 라고 정의되어 있다. In the Food Code of the Republic of Korea, the term "source" refers to a mixture of spices, herbs, sugars, salt, vinegar, etc. added to the raw materials of animals and plants, or aged, Is defined as 'what is said to be'.
우리나라는 곡식을 주원료로 하는 식생활로 소스류보다 양념류가 크게 발달하였으며, 양념은 음식을 만들 때에 식품이 지닌 고유한 맛을 살리면서 음식마다 특유한 맛을 내는데 여러 가지 재료가 사용된다. In Korea, the main ingredient of the food is cereals, and the seasonings are more developed than the sauces. Spices are made of various materials in order to produce the unique taste of the food while making the food.
육류용 소스에 관한 선행 기술로서는 대한민국 공개특허 제10-2005-0035993호는 삼백초를 이용하여 육류 및 생선을 연화시킬 수 있는 소스를 제안함으로써, 육류의 소화흡수를 돕고, 육류로 인한 비만의 발생을 억제하였으며, 대한민국 공개특허 제10-2010-0118208호에서는 울금을 함유한 육류 및 생선용 양념소스 조성물을 제안하여 육류와 생성의 비린내를 제거해 줄뿐만 아니라, 뇌졸증을 예방하고 비타민을 보충해 주며 항암 효과를 나타내도록 하였다. Korean Patent Laid-Open No. 10-2005-0035993 proposes a sauce capable of softening meat and fish by using Saururus chinensis as a prior art for meat sauce, thereby helping digestion and absorption of meat, Korean Patent Laid-Open No. 10-2010-0118208 proposes a seasoning sauce composition for meat and fish containing ugum to eliminate the fishy and raw fishy smell, as well as to prevent stroke, supplement vitamins, Respectively.
상기 선행기술들은 기능성은 더하였으나 육류 요리의 풍미에 좋지 않은 영향을 미쳐 그 맛과 향이 좋지 못해 기호도가 감소하는 단점이 있었다. The above-mentioned prior arts have a disadvantage in that although the functionalities are added, they have a bad influence on the flavor of meat dishes, and taste and flavor are not good.
이에, 요리의 풍미 및 기호도를 향상시키기 위한 기술로서, 대한민국 공개특허 제10-1644617호와 대한민국 공개특허 제10-1606113호에서는 육류의 노린내를 제거하고 연육 작용하여 전체적인 육류 요리의 풍미 및 식감을 개선시키기 위한 육류용 포도소스 조성물의 그의 제조방법을 제시하고 있다.As a technology for improving the flavor and preference of a dish, Korean Patent Laid-Open No. 10-1644617 and Korean Patent Publication No. 10-1606113 disclose a method for improving the flavor and texture of whole meat dishes And a method for producing the grape sauce composition for meat.
하지만, 상기 선행기술들은 돼지고기와 쇠고기 등의 육류에 대한 맛과 기능성을 더하고 풍미 둥의 개선을 위한 것으로서, 최근 소비가 급증하고 있는 닭고기 및 닭꼬치용 소스에 대한 기술은 미미하다. However, the above-mentioned prior arts are aimed at improving the flavor and functionality of meat such as pork and beef and improving flavor, and there is little technology for sauce for chicken and chicken skewers, which have recently been consumed rapidly.
한편, 대추는 갈매나무과 대추나무의 열매로 중국계 대추(Zizyphus jujuba)와 인도계 대추(Zizyphus mauritiana LAM)가 있으며 주로 생태형이 전혀 다른 2종이 세계적으로 재배되고 있다. 유럽 남부, 아시아 남부 및 동부가 원산지로 우리나라, 중국, 일본에 분포하고 있으며 우리나라에서는 재래종인 복조, 보은, 산조대추 등이 분포하고 있다. 대추 잎은 타원형이며 매끄럽고 세 개의 잎맥이 뚜렷하고 타원형의 열매를 맺는데 껍질이 얇고 녹색을 띠지만 익으면서 적갈색으로 변화가 된다. 대추는 상쾌한 맛과 독특한 향을 가지고 있으나 수확기간이 짧고 익으면서 쉽게 연화되는 성질이 있어, 생과로서는 저장성이 낮으므로 주로 건조하여 이용되고 있다. 건조된 대추는 저장성이 높아 이용 폭이 넓으며 과용에 따른 부작용도 없는 과실이다. On the other hand, jujube is the fruit of the seagrass and jujube,Zizyphus jujuba) And Indian jujube (Zizyphus mauritiana LAM), two species that are largely ecologically different are cultivated globally. It is distributed in Korea, China and Japan. It is distributed in southern Europe, southern Asia, and eastern part. In Korea, native species such as demodulation, boeun, and mountain jujube are distributed. The jujube leaves are elliptical, smooth, and three veins are distinctive. The leaves are elliptical. The leaves are thin and green, but they change to reddish brown. Jujube has a refreshing taste and unique fragrance, but it has a short harvesting period and is easy to soften while ripe. Dried jujube has high storage stability and wide range of use. It has no side effects due to overuse.
예로부터 대추는 한방에서 감미를 내기 위하여 첨가되는 경우가 많았으며, 그 자체로도 위경련, 불면증. 소화불량, 대장하혈, 지각과민증의 증상을 개선시키는 약효를 지니고 있다. 또한 쇠약한 내장의 기능을 회복시키고 전신을 튼튼하게 해주며 신경을 안정시키고 노화를 방지하는 항산화효과가 있다. 민간에서는 잘 익은 대추를 쪄서 말렸다가 달여 먹으면 열을 내리는 해열효과와 변비를 없애고 기침을 멎게 하는 것으로 전래되어 왔다. Jujube has been added for sweetening in many oriental foods from time to time. Dyspepsia, colonic hyperplasia, and hypersensitivity symptoms. It also has the antioxidant effect that restores the function of the weakened intestines, strengthens the whole body, stabilizes the nerves and prevents aging. In the private sector, it has been known that steamed and dried ripe jujube, then eaten for a month to eliminate fever and constipation to stop fever and stop the cough.
대추의 과육 가운데 가장 풍부한 성분은 당질로, 생과에서 24-31%, 건 대추에서 58-65% 정도의 단당류와 다당류가 들어있으며, 주된 당류로는 과당, 포도당, 오리고당, 아미노산, 사과산, 폴리페놀, 비타민 A, B, C등이 있어 약리 효과가 높고, 사포닌도 대추의 주용 성분으로서 과실, 종자, 뿌리 등에 골고루 함유되어 있다. 대추가 가지고 있는 비타민류, 식이성 섬유, 플라보노이드, 미네랄 등은 노화방지와 항암효과가 있고, 대추는 발암물질을 흡착, 배출하는 좋은 식이섬유를 갖고 있으며, 베타카로틴은 체내 유해활성 사노를 제거하는 기능도 있다. 이처럼 '대추를 보고 먹지 않으면 늙는다'라는 옛 말처럼 대추는 페놀성 물질이 풍부한 기능성 식품으로 알려져 있다. The most abundant component of jujube in the jujube is saccharides, which contains 24-31% of raw sugar and 58-65% of monosaccharides and polysaccharides in dried jujube. The main sugars are fructose, glucose, oligosaccharides, amino acids, Phenol, and vitamins A, B, and C, which have a high pharmacological effect. Saponin is also a major component of jujube, and is evenly distributed in fruits, seeds and roots. Vitamin, dietary fiber, flavonoid, and minerals possessed by jujube have antioxidant and anticancer effects. Jujube has good dietary fiber that adsorbs and excretes carcinogens. Beta carotene eliminates harmful active substances in the body. Function. Like this old saying, "If you do not eat jujube and get old," jujube is known as a functional food rich in phenolic substances.
대추에 관한 선행연구로는 생대추를 이용한 와인의 발효특성, 대추 페이스트를 첨가한 약밥 품질특성, 대추 고추장의 품질 특성, 대추 농축액을 첨가한 절편의 품질특성, 대추의 저장성에 관한 연구, 대추 추출물의 기능성에 관한 연구, 대추재배에 관련된 연구 등 대부분 건조대추에 대한 연구가 많았으며 대추 씨 생리활성이 고지혈증 흰쥐의 생체 내 지질대사에 미치는 영향, 대추 메탄올 추출액이 Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 영향 등 보고되었다. The previous studies on jujube fermentation showed that fermentation characteristics of wine, fermentation quality with jujube paste, quality characteristics of jujube kochujang, quality characteristics of jujube with jujube concentrate, storage stability of jujube, (A) pyrene-induced hepatic metabolism in rats fed hyperlipidemic rats. The effects of dietary β-carotene on the lipid metabolism in hyperlipidemic rats were investigated. And the effect on functional impairment were reported.
유산균은 인간이 이용할 수 있는 가장 유익한 미생물 중의 한 종류로서, 비병원성균으로 인간의 장내에 서식하면서 정장 및 정균작용을 할 수 있고 당류를 발효해서 다량의 젖산을 생성하고 낮은 pH 및 혐기적인 조건에서도 잘 생육하며 여러 가지 영양물질을 요구하는 등의 특징을 가지고 있다. 유산균에 의해 채소류가 발효되면 독특한 향과 맛을 내게 되어 관능적 특성이 향상되고, 유기산에 의하여 비타민 C(vitamin C)와 여러 생리활성물질이 잘 보존되며, 초기 재료에는 거의 존재하지 않았던 비타민 B12와 비타민 K가 합성되고 부패균과 병원성균의 성장과 증식을 저해하여 위생적인 식품이 되는 등의 장점을 가지고 있는 것으로 알려져 있다. Lactic acid bacteria is one of the most beneficial microorganisms that can be used by humans. It is a non-pathogenic bacterium that can function in the intestines of humans and can function as a bacterium and bacterium. It ferments sugars to produce a large amount of lactic acid. And it requires a variety of nutrients. When fermented by the lactic acid bacteria, the flavor and flavor are enhanced, and the sensory characteristics are enhanced. Vitamin C (vitamin C) and various physiologically active substances are well preserved by the organic acid. Vitamin B 12 It is known that vitamin K is synthesized and inhibits the growth and proliferation of spoilage bacteria and pathogenic bacteria, resulting in hygienic food.
상기와 같은 문제점을 해결하기 위한 본 발명의 목적은 특정의 조건에서 추출한 대추에 분리 동정 후 기탁된 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP) 유산균주를 접종하고 발효시킨 발효물을 획득하고 이 발효물을 이용하여 닭고기용 소스 조성물을 제조하고 이를 닭고기 꼬치용 조리에 적용할 수 있도록 하는 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법을 제공하는 것이다.In order to solve the above problems, the object of the present invention is to provide a process for producing Lactobacillus spp. plantarum DUIJ-16112, KCTC13170BP) obtained by fermenting a fermented product obtained by inoculating and fermenting a lactic acid bacterium, preparing a source composition for chicken using the fermented product, and applying the fermented product to a chicken skewer And to provide a sauce composition for chicken skewers and a process for producing the same.
상기 과제를 해결하기 위한 본 발명의 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물의 제조방법은 대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 유산균 발효 대추 추출물을 제조하는 유산균 발효 대추 추출물 제조단계(S100)와; 상기 유산균 발효 대추 추출물에 물엿, 과당, 간장을 혼합하여 제1혼합물을 형성하는 제 1혼합단계(S200)와; 상기 제 1혼합물에 토마토를 첨가하여 제 2혼합물을 형성하는 제 2혼합단계(S300)와; 상기 제 2혼합물에 후추, 다진마늘, 생강을 혼합하여 제 3혼합물을 형성하는 제3혼합단계(S400)와; 상기 제 3혼합물에 전분, 표고버섯 분말, 가라기난을 혼합하여 제 4혼합물을 형성하는 제4혼합단계(S500)와; 상기 제 4혼합물을 60 내지 100℃로 가열하는 가열단계(S600)를 포함하는 것을 특징으로 한다.In order to solve the above problems, the present invention provides a method of preparing a sauce composition for chicken skewers using fermented product of lactic acid bacteria of jujube, which comprises inoculating and fermenting Lactobacillus plantarum (Lactobacillus plantarum DUIJ-16112, KCTC13170BP) A fermented jujube extract preparation step (SlOO) for producing an extract; A first mixing step (S200) of mixing the lactic acid bacteria fermented jujube extract with starch syrup, fructose, and soy sauce to form a first mixture; A second mixing step (S300) of adding tomato to the first mixture to form a second mixture; A third mixing step (S400) of mixing pepper, chopped garlic, and ginger to the second mixture to form a third mixture; A fourth mixing step (S500) of mixing starch, mushroom powder, and garachan to the third mixture to form a fourth mixture; And a heating step (S600) of heating the fourth mixture to 60 to 100 占 폚.
상기 유산균 발효 대추 추출물 제조단계(S100)는 대추를 절단하여 소정의 온도와 시간에서 대추 추출물을 추출하는 대추 추출물 추출단계(S110)와; 상기 대추 추출물에 0.1 내지 10 부피%의 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종하는 분리 유산균주 접종단계(S120)와; 유산균 접종된 대추 추출물을 발효하는 발효단계(S130)와; 발효된 대추 추출물을 살균하는 발효물 살균단계(S140)를 포함하는 것을 특징으로 한다.The step (S100) of extracting jujube fermented jujube extract comprises extracting jujube extract (S110) which extracts jujube extract at a predetermined temperature and time by cutting jujubes; Step S120 of inoculating the jujuba extract with 0.1 to 10% by volume of lactic acid bacteria Lactobacillus plantarum DUIJ-16112, KCTC13170BP; A fermentation step (S130) of fermenting the jujube extract inoculated with lactic acid bacteria; Sterilization step (S 140) of fermenting the fermented jujube extract to sterilize the fermented jujube extract.
상기 대추 추출물 추출단계(S110)는 대추를 절단하여 95℃, 6시간에서 10 브릭스(°brix)의 농도로 추출하고, pH 5.0 ~ 7.0으로 조정한 후 살균 처리하는 것을 특징으로 한다.The jujube extract is extracted at a concentration of 10 brix at 95 ° C for 6 hours, adjusted to a pH of 5.0 to 7.0, and then sterilized.
이때, 상기 대추 추출물은 상술된 것 이외에 대추 분쇄액, 대추 농축액, 대추 건조물, 대추 과립 중 어느 하나 이상으로 부터 제조 및 준비될 수 있다.At this time, the jujube extract may be prepared and prepared from any one or more of jujube powder, jujube concentrate, dried matter of jujube, and jujube granules in addition to the above.
상기 발효단계(S130)는 유산균 접종된 대추 추출물을 20∼45℃, 6∼120시간 발효하는 것을 특징으로 한다.The fermentation step (S130) is characterized in that the jujube extract inoculated with lactic acid bacteria is fermented at 20 to 45 DEG C for 6 to 120 hours.
상기 발효물 살균단계(S140)는 발효된 대추 추출물을 60∼100℃, 10∼60분간 가열 살균 처리하고, 1.0 마이크로 필터 여과기에서 여과시키는 것을 특징으로 한다. In the fermentation sterilization step (S140), the fermented jujube extract is heated and sterilized at 60 to 100 DEG C for 10 to 60 minutes, and filtered through a 1.0 microfilter filter.
상기 제 1혼합단계(S200)는 유산균 발효 대추 추출물 100중량부에 대하여, 물엿 5∼15중량부, 과당 1∼10중량부, 간장 3∼10중량부를 혼합하는 것을 특징으로 한다. In the first mixing step (S200), 5 to 15 parts by weight of starch syrup, 1 to 10 parts by weight of fructose, and 3 to 10 parts by weight of soy sauce are mixed with 100 parts by weight of the lactic acid bacteria fermented jujube extract.
상기 제 2혼합단계(S300)는 유산균 발효 대추 추출물 100중량부에 대하여, 토마토 5∼30중량부를 혼합하는 것을 특징으로 한다.In the second mixing step (S300), 5 to 30 parts by weight of tomatoes are mixed with 100 parts by weight of the jujube fermented jujube extract.
상기 제3혼합단계(S400)는 유산균 발효 대추 추출물 100중량부에 대하여, 후추 0.1∼0.2중량부, 다진마늘 0.1∼0.5중량부, 생강 0.1∼02 중량부를 혼합하는 것을 특징으로 한다.In the third mixing step (S400), 0.1 to 0.2 parts by weight of pepper, 0.1 to 0.5 parts by weight of chopped garlic, and 0.1 to 0 parts by weight of ginger are mixed with 100 parts by weight of the lactic acid fermented jujube extract.
상기 제4혼합단계(S500)는 유산균 발효 대추 추출물 100중량부에 대하여, 전분 5∼10중량부, 표고버섯 분말 3∼10중량부, 가라기난 3∼10중량부를 혼합하는 것을 특징으로 한다.In the fourth mixing step (S500), 5 to 10 parts by weight of starch, 3 to 10 parts by weight of mushroom powder, and 3 to 10 parts by weight of carrageenan are mixed with 100 parts by weight of the lactic acid fermented jujube extract.
상기 과제를 해결하기 위한 본 발명의 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물은 대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 제조된 유산균 발효 대추 추출물 100중량부에 대하여 물엿 5~15 중량부, 과당 1~10 중량부, 간장 3~10 중량부, 토마토 5~30 중량부, 후추 0.1~0.2 중량부, 다진마늘 0.1~0.5 중량부, 생강 0.1~0.2 중량부, 전분 5~10 중량부, 표고버섯 분말 3~10 중량부, 가라기난 3~10 중량부를 포함하는 것을 특징으로 한다.In order to solve the above problems, the source composition for chicken skewers using fermented lactic acid bacteria of jujube according to the present invention is prepared by inoculating and fermenting lactic acid bacteria Lactobacillus plantarum DUIJ-16112, KCTC13170BP into jujube extract, 5 to 15 parts by weight of starch syrup, 1 to 10 parts by weight of fructose, 3 to 10 parts by weight of soy sauce, 5 to 30 parts by weight of tomato, 0.1 to 0.2 parts by weight of pepper, 0.1 to 0.5 parts by weight of chopped garlic, 0.1 to 0.5 parts by weight of ginger To 5 parts by weight of starch, 3 to 10 parts by weight of mushroom powder, and 3 to 10 parts by weight of carrageenan.
상술한 바와 같이, 본 발명에 따른 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에 의하면 김치류 및 침채류로부터 분리 동정한 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-1612, 수탁번호 : KCTC13170BP)을 대추 추출물에 접종 및 발효시켜 제조된 유산균 발효 대추 추출물을 닭고기용 및 닭고기 꼬치용 소스 제조에 사용함으로써 닭고기 특유의 냄새 저감하고 맛, 풍미 및 기호도를 향상시킨 효과가 있다. As described above, according to the sauce composition for chicken skewers using the fermented product of lactic acid bacteria of jujubes according to the present invention and the method for producing the same, lactic acid bacteria Lactobacillus ( Lactobacillus, plantarum DUIJ-1612, accession no.: KCTC13170BP) was inoculated into fermented jujube extract and fermented to produce sauce for chicken and chicken skewers. It is effective.
도 1은 본 발명에 따른 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에서 대추추출액의 유산균 발효물과 소스 제조단계를 개략적으로 나타낸 흐름도.
도 2는 본 발명에 따른 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에서 1차분리된 유산균들(균주번호 L-1∼L-19)의 에스큘린 배지에서 에스큘린 반응사진.
도 3은 본 발명에 따른 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물과 그의 제조방법에서 표준균주와 최종분리균주 L1(붉은색)의 16S rRNA 유전자 시퀸스 기반으로 한 계통수(phylogenetic tree)의 근연관계도.1 is a flow chart schematically showing a fermented product of lactic acid bacteria and a source preparation step of a jujube extract in a sauce composition for chicken skewers using the fermented product of lactic acid bacteria of jujubes according to the present invention and its preparation method.
FIG. 2 is a photograph showing an esculin reaction in an Escherichia culture medium of lactic acid bacteria (strain Nos. L-1 to L-19) firstly isolated in a source composition for chicken skewers using the fermented product of lactic acid bacteria of jujub .
FIG. 3 is a graph showing the relationship between the phylogenetic tree based on the 16S rRNA gene sequence of the standard isolate and the final isolate L1 (red) in the source composition for chicken skewers using fermented lactic acid bacteria of jujubes according to the present invention, Relationship diagram.
본 발명의 구체적 특징 및 이점들은 이하에서 첨부도면을 참조하여 상세히 설명한다. 이에 앞서 본 발명에 관련된 기능 및 그 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 구체적인 설명을 생략하기로 한다.Specific features and advantages of the present invention will be described in detail below with reference to the accompanying drawings. The detailed description of the functions and configurations of the present invention will be omitted if it is determined that the gist of the present invention may be unnecessarily blurred.
도 1은 본 발명의 대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물의 제조방법을 보여주는 순서도로서, 대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 유산균 발효 대추 추출물을 제조하는 유산균 발효 대추 추출물 제조단계(S100)와, 상기 유산균 발효 대추 추출물에 물엿, 과당, 간장을 혼합하여 제1혼합물을 형성하는 제 1혼합단계(S200)와, 상기 제 1혼합물에 토마토를 첨가하여 제 2혼합물을 형성하는 제 2혼합단계(S300)와, 상기 제 2혼합물에 후추, 다진마늘, 생강을 혼합하여 제 3혼합물을 형성하는 제3혼합단계(S400)와, 상기 제 3혼합물에 전분, 표고버섯 분말, 가라기난을 혼합하여 제 4혼합물을 형성하는 제4혼합단계(S500)와, 상기 제 4혼합물을 60 내지 100℃로 가열하는 가열단계(S600)를 포함하는 것을 특징으로 한다.FIG. 1 is a flow chart showing a method of preparing a sauce composition for chicken skewers using fermented lactic acid bacteria of jujube according to the present invention. Inoculation and fermentation of lactic acid bacteria Lactobacillus plantarum DUIJ-16112 and KCTC13170BP into jujube extract, A first mixing step (S200) of producing a jujube fermented jujube extract to produce a jujube extract, a first mixing step (S200) of mixing the fermented jujube extract of lactic acid bacteria with syrup, fructose and soy sauce to form a first mixture, A third mixing step (S400) of mixing pepper, chopped garlic and ginger to form a third mixture (S400); and a second mixing step (S500) in which starch, mushroom powder and carrageenan are mixed to form a fourth mixture, and a heating step (S600) in which the fourth mixture is heated to 60 to 100 占 폚. .
1. 유산균의 분리1. Isolation of lactic acid bacteria
1.1. 유산균의 분리1.1. Isolation of lactic acid bacteria
유산균 분리에 사용한 발효식품 시료는 가정집 및 음식점에서 담근 김치 수십 종 및 침채류 등을 수집하여 시료액으로 사용하였으며, 각 시료액을 멸균증류수로 10-1 ∼ 10-5으로 희석한 후 0.004% BCP가 첨가된 시판용 MRS 한천배지에 100 μl 씩 분주 도말하고, 37℃에서 24시간 배양 후 나타난 독립된 콜로니 중 유산균의 특징적인 콜로니를 순수 분리하였다. 순수 분리한 유산균은 MRS 한천사면배지에 접종하여 37℃에서 24시간 배양한 후 4℃ 냉장보관하면서 사용하였다. The fermented food samples used for the isolation of lactic acid bacteria were collected from dozens of kimchi dipped in households and restaurants and used as sample liquids. Each sample liquid was diluted with sterile distilled water to 10 -1 to 10 -5 , and then 0.004% BCP 100 μl aliquots were added to the commercially available MRS agar medium and cultured for 24 hours at 37 ° C. to isolate characteristic colonies of lactic acid bacteria from isolated colonies. The purely isolated lactic acid bacteria were inoculated on MRS agar slant medium, cultured at 37 ° C for 24 hours, and stored at 4 ° C in a refrigerator.
1.2. 1.2. 최종선별균주의Of the final selection strains 염기서열분석과 계통분류 Sequence analysis and phylogeny
최종 선별된 균주의 16S rRNA gene은 시퀀싱(sequencing) 을 통하여 분석하였으며, NCBI 데이터베이스(database)를 이용하여 분리된 균주와 database상의 표준균주(type strain)와의 유사성(similarity, %)을 확인하였다.The 16S rRNA gene of the finally selected strain was analyzed by sequencing and the similarity (%) between the isolated strain and the type strain on the database was confirmed using the NCBI database.
2. 유산균 발효 대추 추출물 제조2. Production of jujube fermented jujube extract
2.1. 대추 추출물의 수득2.1. Acquisition of Jujube Extract
대추를 흐르는 물에 세척하고 이물질을 제거한 다음 씨를 제거하고 절편하여 추출에 사용하였다. 절편한 대추의 추출은 냉수에 절편한 대추를 10% 첨가하여 95℃, 6시간에서 추출한 대추 추출물에서 10 브릭스의 액상의 대추 추출물을 얻어 냉동고에 보관하면서 발효에 사용하였다. The jujube was washed in running water, the foreign matter was removed, and the seeds were removed and used for extraction. The jujube extracts were prepared by adding 10% jujube fractioned in cold water, and extracting jujubes of 10 brix in jujube extracts at 95 ℃ for 6 hours.
2.2. 대추 추출물의 유산균 발효2.2. Lactic acid fermentation of jujube extract
2.2.1. 분리 2.2.1. detach 유산균주의Lactic acid bacteria 대추 추출물의 발효 Fermentation of Jujube Extract
대추 추출액(10브릭스)을 85℃에서 10분으로 멸균하여 실온 서서히 식힌 다음 미리 활성화 시킨 균주를 접종하여 37℃에서 72시간 발효하여 발효특성을 조사하여 발효 활성이 가장 뛰어난 1종의 균주를 선택하였다. The jujube extract (10 brix) was sterilized at 85 ° C for 10 minutes, cooled slowly at room temperature, and then inoculated with a pre-activated strain and fermented at 37 ° C for 72 hours to determine fermentation characteristics. .
2.2.2.2.2.2. 대추 Jujube 발효물의Fermented 당도, pH 및 Sugar content, pH and 적정산도Titratable acidity 측정 Measure
발효 중 조건의 일정 시간별로 시료를 취하여 대추 발효물의 당도는 당도계(Refractometer, PR-201α, ATAGO, Japan)로 측정하였다. 대추 발효물의 pH는 pH meter(Metter Toledo Group, Switzerland)를 사용하여, 적정 산도는 0.1 N NaOH용액으로 pH 8.3으로 중화 적정하여 소비된 0.1 N NaOH용액을 젖산 함량(%)으로 환산하였다. The sugar content of the fermented jujube was measured by a refractometer (PR-201α, ATAGO, Japan). The pH of the jujuba fermented product was converted to the lactic acid content (%) using a pH meter (Metter Toledo Group, Switzerland) and the titratable acidity was neutralized to pH 8.3 with 0.1 N NaOH solution and consumed 0.1 N NaOH solution.
2.2.3.2.2.3. 대추 Jujube 발효물의Fermented 생균수Viable cell count 측정 Measure
대추 발효물의 생균수는 발효 중 일정 시간별로 시료를 무균적으로 취한 후 생리멸균식염수로 적정 희석하여 시판용 MRS 한천배지에 접종하여 37℃에서 24시간 배양 후 나타나는 콜로니 수로 나타내었다.The number of viable cell counts of jujubes fermented by fermentation was aseptically taken for a certain period of time during fermentation, then diluted appropriately with physiological sterilized saline and inoculated on a commercial MRS agar medium and expressed as the number of colonies after incubation at 37 ° C for 24 hours.
2.2.4.2.2.4. 기호도 검사Preference check
소스의 적합성 및 조성물을 결정하기 위하여 대학교 재학생(20대, 20명) 및 일반인(30~40대, 20명)등 총 40명을 대상으로 소스 자체 및 닭 꼬치고기에 소스를 발라 180℃ 오븐에 5분간 구운 것으로 맛의 평가를 실시하였다. 기호도 평가는 외관, 냄새, 맛, 조직감, 전반적인 기호도 항목에 대하여 9점 기호 척도법(매우 좋다=9, 매우 싫다=1)으로 평가하였다. To determine the suitability and composition of sauces, sauce and saute chicken meat were sown on a total of 40 students including university students (20 students, 20 students) and general students (30 students, 40 students, 20 students) The taste was evaluated by baking for 5 minutes. Likelihood evaluation was evaluated by the 9-point symbol scale method (very good = 9, very dissatisfied = 1) for appearance, smell, taste, texture,
3. 유산균 선발3. Selection of lactobacilli
3.1. 발효식품으로부터 유산균 분리3.1. Isolating lactic acid bacteria from fermented foods
발효 김치류 26종의 시료에서 단일콜로니로 분리된 균들 중, MRS 한천배지에서 유산균의 특징을 보이는 콜로니를 형태적으로 다른것을 분류하여 30개의 유산균을 순수 분리하였다. 30개의 유산균을 에스쿨린 한천법을 이용해 실험한 결과는 도 2 에 나타내었다. 30개의 유산균에서 콜로니 주위에 흑색반점(black complex)이 관찰되어 베타-글루코시데이즈(β-Glucosidase) 활성능이 있는 것으로 판단되었고, 에스쿨린의 반응을 보인 16개의 균주 중 119개는 흑색반점의 직경의 크기가 5mm 이하였고, 그중 반복실험을 통해 약 15mm이상의 흑색반점(도 1에 도시)을 갖는 7개 균주를 분리하였다(표 1).Among 30 strains isolated from a single colony in 26 fermented kimchi samples, 30 strains of lactic acid bacteria were isolated by morphologically differentiating colonies showing characteristics of lactic acid bacteria on MRS agar medium. The results of 30 lactic acid bacteria experiments using the Esculin agar method are shown in Fig. In 30 lactic acid bacteria, a black complex was observed around the colonies and it was judged to have beta-glucosidase activity. Of the 16 Escherichia coli strains, 119 were found to have black spot The size of the diameter was 5 mm or less. Seven strains having black spots (shown in FIG. 1) of about 15 mm or more were isolated through repeated experiments among them (Table 1).
3.2. 대추 추출물 발효를 위한 3.2. For jujube extract fermentation 유산균주Lactic acid bacteria 선택 Selection
대추 추출액을 10 브릭스로 조정 후 분리된 8종의 유산균주를 배양 접종하여 37℃에서 48 시간 배양하였다. 균주별 대추 발효물의 이화학적 성분 검사 결과는 표 2에 나타내었다. 성분 검사 결과, 8종의 균주 중 산 생성력과 대추 발효물내 유산균 생균수가 가장 높게 나타난 유산균 L-1번을 최종 발효유산균으로 선택하였다. After adjusting the jujube extract to 10 brix, 8 isolates of lactic acid bacteria were inoculated and cultured at 37 ° C for 48 hours. The results of physicochemical examination of jujube fermented by strain are shown in Table 2. As a result of the test, L-1 was selected as the final fermented lactic acid bacterium in which the acid production ability and the highest number of lactic acid bacteria in juju fermented product were found among the 8 strains.
4. 분리유산균의 동정4. Identification of isolated lactic acid bacteria
최종 선택된 균주 중 활성능이 가장 높은 25번 균주의 16S rRNA gene의 부분 염기서열을 분석하여 NCBI blast DB와 비교한 결과는 표 3과 같다. 그 결과 데이터베이스의 비교균주로 락토바실러스 프란타럼(Lactobaciilus plantarum WCFS1 n 등 Lactobaciilus plantarum 의 균주와 100%의 유사성을 보였다. 그리고 상기 데이터베이스 연관균주와 분리균주의 16S rRNA 유전자 시퀸스 기반으로 한 계통수(phylogenetic tree)의 근연관계도는 도 3과 같다. 이들의 결과를 근거로 최종분리된 균주를 Lactobaciilus plantarum으로 동정하고 Lactobaciilus plantarum DUIJ-1612로 최종 명명하였으며 본 균주를 한국생명공학연구원 생물자원센터에 특허 기탁하여 수탁번호 KCTC13170BP를 부여받았다.The partial sequence of the 16S rRNA gene of the 25th most active strain among the finally selected strains was analyzed and compared with the NCBI blast DB. As a result, a comparative strain of Lactobacillus plantarum ( Lactobacillus plantarum WCFS1 n) was 100% similar to a strain of Lactobacillus plantarum as a comparative strain of the database, and a phylogenetic tree based on 16S rRNA gene sequence of the database- of allied relations are illustrated in Figure 3 to identify the final strains on the basis of these results Lactobaciilus plantarum and was the final named Lactobaciilus plantarum DUIJ-1612 patents deposited this strain in the Korea Research Institute of Bioscience and biotechnology biological resource Center trustee No. KCTC13170BP.
5. 대추 추출액을 활용한 분리젖산균 발효조건 검토5. Review of Fermentation Conditions of Isolated Lactic Acid Bacteria Using Jujube Extract
대추 추출액을 10 브릭스로 조정 후 최종 분리된 L-1종의 유산균주를 배양 접종하여 37℃에서 72 시간 동안 배양하였다. 72시간 배양기간중의 대추 발효물의 이화학적 성분 검사 결과는 표 4에 나타내었다. 성분 검사 결과 발효 48시간 이후 거의 균 성장과 발효물의 변화가 없는 것으로 나타나 배양 시간 48시간동안 발효 한 것을 이 후 실시 발효물로 사용하였다.After adjusting the jujube extract to 10 brix, the final isolated L-1 species of lactic acid bacteria were inoculated and cultured at 37 ° C for 72 hours. The results of physicochemical examination of jujubes fermented during 72 hours incubation period are shown in Table 4. After 48 hours of fermentation, the fermented product was fermented for 48 hours after fermentation.
상기의 표 4에 따른 유산균 발효액을 그대로 또는 살균하여 다음의 제조예의 소스를 제조하여 그 조성액을 조제하고 40명의 기호도 검사를 통하여 최적의 조성액을 조사하였다. The lactic acid fermentation broth according to Table 4 was or sterilized as it was to prepare the sauces of the following preparations, and the composition was prepared.
조제예Preparation example 1 One
상기의 대추 발효액 또는 살균액 100 중량부과 물엿 5중량부, 과당 1중량부, 간장 3중량부, 토마토 5중량부, 후추 0.1중량부, 다진마늘 0.1 중량부, 생강 0.1 중량부, 전분 5중량부, 표고버섯 분말 3중량부, 가라기난 3 중량부를 혼합하여 30분 방치후 닭꼬지 10 g에 펼쳐 바르고 180℃ 오븐에 구워 조리하였다.100 parts by weight of the jujube fermentation broth or the sterilizing liquid and 5 parts by weight of starch syrup, 1 part by weight of fructose, 3 parts by weight of soy sauce, 5 parts by weight of tomato, 0.1 part by weight of pepper, 0.1 part by weight of minced garlic, 0.1 part by weight of ginger, , 3 parts by weight of shiitake mushroom powder and 3 parts by weight of carrageenan were mixed and allowed to stand for 30 minutes. The mixture was spread on 10 g of chicken broth and cooked in an oven at 180 ° C.
조제예Preparation example 2 2
상기의 대추 발효액 또는 살균액 100 중량부과 물엿 7중량부, 과당 5중량부, 간장 5중량부, 토마토 10중량부, 후추 0.15중량부, 다진마늘 0.2중량부, 생강 0.13중량부, 전분 7중량부, 표고버섯 분말 5중량부, 가라기난 5중량부를 혼합하여 30분 방치후 닭꼬지 10 g에 펼쳐 바르고 180℃ 오븐에 구워 조리하였다.10 parts by weight of tomato, 5 parts by weight of soy sauce, 10 parts by weight of tomato, 0.15 part by weight of pepper, 0.2 parts by weight of minced garlic, 0.13 parts by weight of ginger, 7 parts by weight of starch, , 5 parts by weight of mushroom powder, and 5 parts by weight of carrageenan were mixed and allowed to stand for 30 minutes. The mixture was spread on 10 g of chicken broth and cooked in an oven at 180 캜.
조제예Preparation example 3 3
상기의 대추 발효액 또는 살균액 100 중량부과 물엿 10중량부, 과당 7중량부, 간장 7중량부, 토마토 20중량부, 후추 0.17중량부, 다진마늘 0.35중량부, 생강 0.15중량부, 전분 8중량부, 표고버섯 분말 7중량부, 가라기난 7중량부를 혼합하여 30분 방치후 닭꼬지 10 g에 펼쳐 바르고 180℃ 오븐에 구워 조리하였다.10 parts by weight of starch fermentation broth or sterilization liquid, 10 parts by weight of starch syrup, 7 parts by weight of fructose, 7 parts by weight of liver, 20 parts by weight of tomato, 0.17 part by weight of pepper, 0.35 part by weight of minced garlic, 0.15 part by weight of ginger, , 7 parts by weight of mushroom powder, and 7 parts by weight of Garragana were mixed and allowed to stand for 30 minutes. The mixture was spread on 10 g of chicken broth and cooked in an oven at 180 캜.
조제예Preparation example 4 4
상기의 대추 발효액 또는 살균액 100 중량부과 물엿 15중량부, 과당 10중량부, 간장 10중량부, 토마토 30중량부, 후추 0.2중량부, 다진마늘 0.5중량부, 생강 0.2중량부, 전분 10중량부, 표고버섯 분말 10중량부, 가라기난 10중량부를 혼합하여 30분 방치후 닭꼬지 10 g에 펼쳐 바르고 180℃ 오븐에 구워 조리하였다.10 parts by weight of fructose, 10 parts by weight of soy sauce, 30 parts by weight of tomato, 0.2 part by weight of pepper, 0.5 part by weight of ground chopped garlic, 0.2 part by weight of ginger, 10 parts by weight of starch, , 10 parts by weight of mushroom powder, and 10 parts by weight of Garragana were mixed and allowed to stand for 30 minutes. The mixture was spread on 10 g of chicken broth and cooked in an oven at 180 캜.
비교예Comparative Example 1 내지 4 1 to 4
상기의 조제예 1 내지 4에서 대추 발효액 또는 살균액 100 중량부 대신에 대추 추출물을 대신하여 첨가하고 제조하여 비교예 1 내지 4로 하여 조제예 1내지 4와같이 소스를 제조하고 혼합하여 30분 방치 후 닭꼬지 10 g에 펼쳐 바르고 180℃ 오븐에 구워 비교예 1내지 4를 조리하였다.In the above Preparation Examples 1 to 4, instead of the jujube fermentation broth or 100% by weight of the germicidal liquid, the jujube extract was added and prepared. As Comparative Examples 1 to 4, sauces were prepared and mixed as in Preparation Examples 1 to 4, Then, the mixture was spread on a 10 g portion of chicken meat and baked in an oven at 180 DEG C to prepare Comparative Examples 1-4.
6. 관능 평가6. Sensory Evaluation
상기의 조제예와 비교예를 제조하여 40명의 일반인을 대상으로 관능검사를 실시하여 기호도를 조사하였다. 관능검사는 관능적 특성 총 9점 점수법으로 맛, 식감, 향, 및 전체적인 기호도를 조사하였고 매우 좋음 9점, 매우 싫음 1점으로 하여 나타내었다. 점수는 순차 감소 평가법으로 하여 평균값을 소수 둘째자리에서 반올림한 값으로 표시하였다. 관능검사시 모든 시료는 상온에서 준비하여 평가하였고 각 시료 10 g 을 입안에 넣고 씹어 구강내부를 자극하고 뱉어내도록 하여 평가하고 다음 시료를 평가하기전 물로 헹구어 내고 10분후 다음 시료를 평가하였다. 관능평가의 평균값은 아래 표 5에 나타내었다.The preparation examples and the comparative examples were prepared and 40 people were subjected to sensory evaluation and their preference was examined. The sensory evaluation was evaluated by taste score, texture, flavor, and overall acceptability with a total score of 9 points. Very good score was 9 points, and 1 point was very disliked. The scores were expressed as the value obtained by rounding off the mean value to the second decimal place using the sequential decreasing method. All samples were prepared and evaluated at room temperature. 10 g of each sample was placed in the mouth, chewed to stimulate the inside of the mouth and spit out. The next sample was rinsed with water before evaluation and the next sample was evaluated after 10 minutes. The average value of the sensory evaluation is shown in Table 5 below.
상기 표 5에서 나타난 바와 같이 조제예 1에서 4의 닭꼬치가 향, 맛, 식감, 전체적인 기호도에서 비교예와 비교하여 모두 높은 결과를 보인 것을 알 수 있었다. 따라서 본 기술의 대추 유산균 발효액이 닭꼬치의 관능과 기호도에 영향을 주는 것으로 나타났다. As shown in Table 5, it was found that the chicken skewers of Preparation Examples 1 to 4 exhibited higher aroma, taste, texture and overall acceptability than the comparative examples. Therefore, fermented jujube fermented jujube fermented with jujube fermented by this technology was found to affect sensory evaluation and acceptability of chicken skewers.
이상과 같이 본 발명은, 바람직한 실시 예를 중심으로 설명하였지만 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 본 발명의 특허청구범위에 기재된 기술적 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 또는 변형하여 실시할 수 있다. 따라서 본 발명의 범주는 이러한 많은 변형의 예들을 포함하도록 기술된 청구범위에 의해서 해석되어야 한다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken as a limitation of the scope of the present invention. Or modify it. The scope of the invention should, therefore, be construed in light of the claims set forth to cover many of such variations.
Claims (10)
대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 유산균 발효 대추 추출물을 제조하는 유산균 발효 대추 추출물 제조단계(S100)와;
상기 유산균 발효 대추 추출물 100중량부에 대하여 물엿 5~15 중량부, 과당 1~10 중량부, 간장 3~10 중량부를 혼합하여 제1혼합물을 형성하는 제 1혼합단계(S200)와;
상기 제 1혼합물에 유산균 발효 대추 추출물 100중량부 대비 5~30 중량부의 토마토를 혼합하여 제 2혼합물을 형성하는 제 2혼합단계(S300)와;
상기 제 2혼합물에 유산균 발효 대추 추출물 100중량부 대비 후추 0.1~0.2 중량부, 다진마늘 0.1~0.5 중량부, 생강 0.1~0.2 중량부를 혼합하여 제 3혼합물을 형성하는 제3혼합단계(S400)와;
상기 제 3혼합물에 유산균 발효 대추 추출물 100중량부 대비 전분 5~10 중량부, 표고버섯 분말 3~10 중량부, 가라기난 3~10 중량부를 혼합하여 제 4혼합물을 형성하는 제4혼합단계(S500)와;
상기 제 4혼합물을 60 내지 100℃로 가열하는 가열단계(S600)를 포함하며,
상기 유산균 발효 대추 추출물 제조단계(S100)는
대추를 절단하여 소정의 온도와 시간에서 대추 추출물을 추출하는 대추 추출물 추출단계(S110)와;
상기 대추 추출물에 0.1 내지 10 부피%의 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종하는 분리 유산균주 접종단계(S120)와;
유산균 접종된 대추 추출물을 발효하는 발효단계(S130)와;
발효된 대추 추출물을 살균하는 발효물 살균단계(S140)를 포함하며,
상기 대추 추출물 추출단계(S110)는
대추를 절단하여 95℃, 6시간에서 10 브릭스(°brix)의 농도로 추출하고, pH 5.0 ~ 7.0으로 조정한 후 살균 처리하여 대추 추출물을 수득하며,
상기 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)은 베타-글루코시데이즈(β-glucosidase) 활성을 갖는 것을 특징으로 하는
대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물의 제조방법.
A method for preparing a chicken skewer sauce composition using a lactic acid fermented product of jujube,
A step (S100) of producing a fermented jujube fermented Lactobacillus japonica extract to produce a fermented jujube extract of lactic acid bacteria by inoculating and fermenting lactic acid bacteria Lactobacillus plantarum DUIJ-16112, KCTC13170BP into a jujube extract;
A first mixing step (S200) of mixing 5 to 15 parts by weight of starch syrup, 1 to 10 parts by weight of fructose, and 3 to 10 parts by weight of soy sauce with respect to 100 parts by weight of the lactic acid fermented jujube extract;
A second mixing step (S300) of mixing the first mixture with 5 to 30 parts by weight of tomatoes per 100 parts by weight of the fermented jujube extract of lactic acid bacteria to form a second mixture;
A third mixing step (S400) of mixing 0.1 to 0.2 parts by weight of pepper, 0.1 to 0.5 parts by weight of chopped garlic and 0.1 to 0.2 parts by weight of ginger to 100 parts by weight of the lactic acid bacteria fermented jujube extract, ;
5 to 10 parts by weight of starch, 3 to 10 parts by weight of mushroom powder and 3 to 10 parts by weight of carrageenan are mixed with 100 parts by weight of lactic acid bacteria fermented jujube extract to form a fourth mixture (S500 )Wow;
And a heating step (S600) of heating the fourth mixture to 60 to 100 DEG C,
The lactic acid fermented jujube extract preparation step (SlOO)
A jujube extract extraction step (S110) of cutting a jujube to extract a jujube extract at a predetermined temperature and time;
Step S120 of inoculating the jujuba extract with 0.1 to 10% by volume of lactic acid bacteria Lactobacillus plantarum DUIJ-16112, KCTC13170BP;
A fermentation step (S130) of fermenting the jujube extract inoculated with lactic acid bacteria;
Sterilization step (S 140) of sterilizing fermented jujube extract,
The jujube extract extraction step (S110)
The jujube was cut, and extracted at a concentration of 10 brix (brix) at 95 ° C for 6 hours, adjusted to a pH of 5.0 to 7.0, and then sterilized to obtain a jujube extract,
The lactic acid bacteria Lactobacillus plantarum DUIJ-16112 and KCTC13170BP are characterized in that they have beta-glucosidase activity
(JP) METHOD FOR PREPARING SOURCE COMPOSITION FOR PIEZZOKE SKIRT USING LIQUID BIOPLASTIC FERMENTATION IN JAPAN
상기 발효단계(S130)는
유산균 접종된 대추 추출물을 20∼45℃, 6∼120 시간 발효하는 것을 특징으로 하는
대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물의 제조방법.
The method according to claim 1,
The fermentation step (S130)
Wherein the jujube extract inoculated with lactic acid bacteria is fermented at 20 to 45 DEG C for 6 to 120 hours
(JP) METHOD FOR PREPARING SOURCE COMPOSITION FOR PIEZZOKE SKIRT USING LIQUID BIOPLASTIC FERMENTATION IN JAPAN
상기 발효물 살균단계(S140)는
발효된 대추 추출물을 60∼100℃, 10∼60분간 가열 살균 처리하고, 1.0 마이크로 필터 여과기에서 여과시키는 것을 특징으로 하는
대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물의 제조방법.
The method according to claim 1,
The fermentation sterilization step (S140)
Fermented jujube extract is heated and sterilized at 60 to 100 DEG C for 10 to 60 minutes and filtered through a 1.0 microfilter filter
(JP) METHOD FOR PREPARING SOURCE COMPOSITION FOR PIEZZOKE SKIRT USING LIQUID BIOPLASTIC FERMENTATION IN JAPAN
대추 추출물에 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종 및 발효시켜 제조된 유산균 발효 대추 추출물 100중량부에 대하여 물엿 5~15 중량부, 과당 1~10 중량부, 간장 3~10 중량부, 토마토 5~30 중량부, 후추 0.1~0.2 중량부, 다진마늘 0.1~0.5 중량부, 생강 0.1~0.2 중량부, 전분 5~10 중량부, 표고버섯 분말 3~10 중량부, 가라기난 3~10 중량부를 혼합한 후 60 내지 100℃로 가열하여 제조되며,
상기 유산균 발효 대추 추출물은
대추를 절단하여 95℃, 6시간에서 10 브릭스(°brix)의 농도로 추출하고, pH 5.0 ~ 7.0으로 조정한 후 살균 처리하여 대추 추출물을 수득하고, 상기 대추 추출물에 0.1 내지 10 부피%의 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)을 접종한 유산균 접종된 대추 추출물을 발효한 후 살균처리되어 제조되며,
상기 유산균 락토바실러스 프란타럼(Lactobaciilus plantarum DUIJ-16112, KCTC13170BP)은 베타-글루코시데이즈(β-glucosidase) 활성을 갖는 것을 특징으로 하는
대추의 유산균 발효물을 이용한 닭고기 꼬치용 소스 조성물.
In a sauce composition for chicken skewer using fermented lactic acid bacteria of jujube,
5 to 15 parts by weight of starch syrup, 1 to 10 parts by weight of fructose, 3 to 10 parts by weight of soy sauce were added to 100 parts by weight of lactic acid bacteria fermented jujube extract prepared by inoculating and fermenting Lactobacillus plantarum (Lactobacillus plantarum DUIJ-16112, KCTC13170BP) 10 to 30 parts by weight of tomato, 0.1 to 0.2 parts by weight of pepper, 0.1 to 0.5 parts by weight of chopped garlic, 0.1 to 0.2 parts by weight of ginger, 5 to 10 parts by weight of starch, 3 to 10 parts by weight of mushroom powder, And 3 to 10 parts by weight of lead, followed by heating to 60 to 100 占 폚,
The lactic acid fermented jujube extract
The jujube was cut and the juice was extracted at a concentration of 10 brix at 95 ° C for 6 hours, adjusted to a pH of 5.0 to 7.0, and then sterilized to obtain a jujube extract. To the jujube extract, 0.1 to 10% Lactobacillus plantarum (Lactobaciilus plantarum DUIJ-16112, KCTC13170BP) was inoculated with lactic acid bacteria inoculated with jujube extract, fermented and sterilized,
The lactic acid bacteria Lactobacillus plantarum DUIJ-16112 and KCTC13170BP are characterized in that they have beta-glucosidase activity
Sauce composition for chicken skewer using lactic acid fermented product of jujube.
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