KR101897213B1 - Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도 - Google Patents

Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도 Download PDF

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KR101897213B1
KR101897213B1 KR1020160167045A KR20160167045A KR101897213B1 KR 101897213 B1 KR101897213 B1 KR 101897213B1 KR 1020160167045 A KR1020160167045 A KR 1020160167045A KR 20160167045 A KR20160167045 A KR 20160167045A KR 101897213 B1 KR101897213 B1 KR 101897213B1
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South Korea
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sequence
cpf1
crrna
target
protein
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KR1020160167045A
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Korean (ko)
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KR20170068400A (ko
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김진수
허준호
김대식
김정은
김경미
김혜란
구태영
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기초과학연구원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
KR1020160167045A 2015-12-08 2016-12-08 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도 KR101897213B1 (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR20150174212 2015-12-08
KR1020150174212 2015-12-08
US201662299043P 2016-02-24 2016-02-24
US62/299,043 2016-02-24
KR20160036381 2016-03-25
KR1020160036381 2016-03-25

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KR1020180017195A Division KR101958437B1 (ko) 2015-12-08 2018-02-12 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도

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KR20170068400A KR20170068400A (ko) 2017-06-19
KR101897213B1 true KR101897213B1 (ko) 2018-09-11

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KR1020160167045A KR101897213B1 (ko) 2015-12-08 2016-12-08 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도
KR1020180017195A KR101958437B1 (ko) 2015-12-08 2018-02-12 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도

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WO (1) WO2017099494A1 (fr)

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US11174470B2 (en) 2019-01-04 2021-11-16 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11273442B1 (en) 2018-08-01 2022-03-15 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
KR20240020336A (ko) 2022-08-04 2024-02-15 성균관대학교산학협력단 PAM 서열에 비의존적인 돌연변이 Cas9 단백질

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WO2016073990A2 (fr) 2014-11-07 2016-05-12 Editas Medicine, Inc. Procédés pour améliorer l'édition génomique médiée par crispr/cas
WO2016182959A1 (fr) 2015-05-11 2016-11-17 Editas Medicine, Inc. Systèmes crispr/cas9 optimisés et procédés d'édition de gènes dans des cellules souches
WO2016201047A1 (fr) 2015-06-09 2016-12-15 Editas Medicine, Inc. Procédés liés à crispr/cas et compositions d'amélioration de la transplantation
AU2016326711B2 (en) 2015-09-24 2022-11-03 Editas Medicine, Inc. Use of exonucleases to improve CRISPR/Cas-mediated genome editing
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
EP3443086B1 (fr) 2016-04-13 2021-11-24 Editas Medicine, Inc. Systèmes d'édition de gènes de molécules de fusion cas9 et leurs procédés d'utilisation
KR101961332B1 (ko) * 2016-07-28 2019-03-22 기초과학연구원 Cas9 단백질 및 가이드 RNA를 포함하는 안질환 치료용 약학 조성물
AU2017313616B2 (en) * 2016-08-19 2022-12-08 Institute For Basic Science Artificially engineered angiogenesis regulatory system
WO2018201086A1 (fr) 2017-04-28 2018-11-01 Editas Medicine, Inc. Procédés et systèmes d'analyse de molécules d'arn
CN110997908A (zh) 2017-06-09 2020-04-10 爱迪塔斯医药公司 工程化的cas9核酸酶
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
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WO2019014564A1 (fr) 2017-07-14 2019-01-17 Editas Medicine, Inc. Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés
EP3715461A4 (fr) * 2017-11-21 2021-09-08 Genkore Co. Ltd. Composition d'édition génomique utilisant un système crispr/cpf1 et son utilisation
JP7075170B2 (ja) * 2018-01-23 2022-05-25 インスティチュート フォー ベーシック サイエンス 延長された単一ガイドｒｎａ及びその用途
CN111788307A (zh) * 2018-03-12 2020-10-16 南京北恒生物科技有限公司 工程化的嵌合向导rna及其用途
KR102177174B1 (ko) 2018-05-18 2020-11-10 울산대학교 산학협력단 Pde6b 유전자가 결손된 망막 변성 동물 모델 및 이의 제조 방법
EP3833751A4 (fr) 2018-08-09 2022-04-27 G+Flas Life Sciences Compositions et procédés de modification du génome avec des protéines cas12a
AU2019319377A1 (en) * 2018-08-09 2021-03-11 G+Flas Life Sciences Novel CRISPR-associated protein and use thereof
CN109666684A (zh) * 2018-12-25 2019-04-23 北京化工大学 一种CRISPR/Cas12a基因编辑系统及其应用
KR102493904B1 (ko) 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
KR102551876B1 (ko) 2019-12-18 2023-07-05 한국생명공학연구원 Cpf1 및 키메릭 DNA-RNA 가이드를 포함하는 유전체 교정 또는 발현 억제용 조성물
KR102471698B1 (ko) * 2020-03-24 2022-11-28 연세대학교 산학협력단 신규한 가이드 rna 및 이를 이용한 코로나바이러스감염증 2019를 진단하는 방법
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CN113373170A (zh) * 2021-04-29 2021-09-10 江西农业大学 一种pFnCpfAb/pCrAb双质粒系统及其应用
CN113969281B (zh) * 2021-12-24 2022-04-01 汕头大学 经修饰的CrRNA片段及非洲猪瘟病毒试剂盒

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11273442B1 (en) 2018-08-01 2022-03-15 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US11761029B2 (en) 2018-08-01 2023-09-19 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
US11174470B2 (en) 2019-01-04 2021-11-16 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
KR20240020336A (ko) 2022-08-04 2024-02-15 성균관대학교산학협력단 PAM 서열에 비의존적인 돌연변이 Cas9 단백질

Also Published As

Publication number Publication date
KR20170068400A (ko) 2017-06-19
KR20180028996A (ko) 2018-03-19
WO2017099494A1 (fr) 2017-06-15
KR101958437B1 (ko) 2019-03-15
WO2017099494A8 (fr) 2017-08-10

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