KR101873159B1 - Composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, which contains extracts of Crinum asiaticum, Trapa japonica and Artemisia annua, wherein the composition according to the present invention has a little adverse effects by using a natural substance as an active ingredient, and has excellent effects of preventing hair loss and promoting hair growth by greatly enhancing the viability of dermal papilla cells through a synergistic effect by optimal combination.

Description

[0001] The present invention relates to a composition for preventing hair loss and promoting hair growth,

The present invention relates to a composition for preventing hair loss and promoting hair growth, and more particularly, to a composition for preventing hair loss and promoting hair growth, which comprises a mugwort extract, a mahogany extract, and a mugwort extract.

Hair loss refers to an increase in abnormally falling hair due to an increase in the proportion of hair growing in the retroperitoneum or the hair follicle of the dormant period, compared with the hair growing in the hair follicle during the growth period of the hair follicle. There are two main types of alopecia: male pattern baldness, alopecia seniles, and androgenic alopecia, which are common in middle-aged men, It starts. Second, it is called alopecia areata. It has alopecia of oval or circular shape. It has alopecia that can occur in adolescence. Hair loss occurs not only in males but also in women, and most of the alopecia occurs as male alopecia regardless of sex. Alopecia areata also occurs in both men and women, but incidence is lower in women than in men.

Causes of hair loss include dysfunctional or proliferative inhibition of dermal papilla cells and geminal matrix cells related to the regulation of hair cycle, excessive action of male hormone, lowered blood flow to blanket, excessive sebum secretion, And bacterium caused by dysfunction of the scalp, genetic factors, aging, and neuroses due to stress, and various causes are known to work alone or in combination.

Recently, hair loss and alopecia are increasing due to environmental factors such as air pollution and mental stress. Such alopecia may cause serious stress to the parties and may interfere with the interpersonal relationship, thereby increasing interest in treatment and prevention of hair loss.

To date, various attempts have been made to treat and prevent alopecia. The external methods include scalp flap surgery, reduction of hair loss site, scalp surgery by tissue expanding technique, and recently developed self - hair grafting. Such a surgical method is disadvantageous in that it is difficult to be widely used because the economic and psychological burden of the patient is very great even if it is excellent in the effect.

Currently, the most widely used formulations are minoxidil-containing preparations approved by the US FDA. Recently, Merck Pharmaceuticals has developed a new type of 5-alpha-reductase inhibitor, finasteride, Propecia) has been released and used. However, in the case of minoxidil, side effects such as itching and irritation occur. In the case of finasteride, when the use is stopped, the problem of returning to the original state within 2 months, side effects such as loss of sexual desire and erectile dysfunction occur, There is a disadvantage that it can not be taken because of the risk of birth defects.

In addition, studies based on natural extracts such as astragalus, mushroom, gingkohwa, kemomail, and extracts such as seaweed, iris, pomegranate, and ginkgo biloba extract have been carried out. However, when these hair growth agents are used in clinical practice, The effect is not very good. Accordingly, there is a continuing need for a search for a hair removal agent or a hair removal agent made of a natural substance with a small side effect while showing an excellent effect (Patent Document 1).

Korean Patent Publication No. 10-2012-0103593

It is an object of the present invention to provide a composition exhibiting excellent effects in preventing hair loss and promoting hair growth. In particular, the present invention provides a composition for preventing hair loss and promoting hair growth, which contains a natural substance such as a mugwort extract, a mahogany extract, and a dog mugwort extract.

In order to achieve the above object,

The present invention provides an external dermatological pharmaceutical composition for preventing hair loss and promoting hair growth, which comprises a mugwort extract, a rhizome extract, and a mugwort extract.

In addition, the present invention provides a cosmetic composition for preventing hair loss and promoting hair growth, comprising a mugwort extract, a rhizome extract, and a mugwort extract.

1-300 parts by weight of the wilt extract and 1-300 parts by weight of the ragweed extract may be contained in 100 parts by weight of the mugwort extract.

1 to 200 parts by weight of Baekryikcho extract may be added to 100 parts by weight of the mugwort extract.

1 to 200 parts by weight of Sasa extract may be further added to 100 parts by weight of the above extract.

The composition according to the present invention has a remarkable effect on prevention of hair loss and stimulation of hair growth by remarkably increasing survival ability of dermal papilla cells through synergistic effect according to optimal combination as well as having few side effects using natural materials as active ingredients.

FIG. 1 shows that in the MTT experiment technique, the dehydrogenase activity of the mitochondria reduces the yellow water-soluble MTT to a purple colored water-insoluble MTT formazan, thereby discriminating the living cells and dead cells from the color change This is a picture showing.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for preventing hair loss and promoting hair growth, the composition comprising a mugwort extract, a rhizome extract, and a mugwort extract.

The composition may further comprise a pomegranate extract or a sage extract.

Munjuran (Crinum I have asiaticum . japonicum BAK .) Is an evergreen perennial herbaceous plant belonging to the family of Daffodils. It is 30-50cm high and its leaves are lanceolate, with flowers blooming in July-September. It grows mainly in the sandy shore of warm coast where sunshine comes in. In Korea, it grows only in Cheju Island. The leaves of Mungusan are effective for analgesia, detoxification, and apoptosis. They are known to exert their effects on headache, joint pain, fever, and hemorrhagic action. Especially, in the case of Munjuri, hair follicle growth of hair follicle is excellent.

Trapa japonica Flerov is a perennial herbaceous plant belonging to the order Hydrocaryaceae. The root is in the mud, the main stem grows to the surface, and many leaves spread from the end to all directions. Flowers are white or red, blooming in July and August, and distributed in reservoirs and ponds all over the country. The fruits of dried fruits contain starch, glucose, protein, etc. Recently, substances acting on multiple liver cancer have also been identified.

Artrmisia annua ) is an annual plant of the genus Chrysanthemum mugwort, which is about 1m in height, which grows on the roads, in the winter, and on the river. It is known that it has excellent antimicrobial activity and sterilizing action, and it is used in the treatment of fever and cold, schizophrenia, pediatric competition, dyspepsia and dysentery.

It is called Opuntia cactus, which is native to Korea, and is also known as an open-handed cactus or a fossil cactus. Nopal and stem (Nopalitos) are both used for food and have been widely used in folk medicine and oriental medicine since ancient times. This cactus is used as an anti-inflammatory and antipyretic medicine, and it is said that drinking Baekryikcho juice causes the stomach that causes vomiting to sink, stop the painful cough and improve the constitution. It also has an effect on constipation. The flesh of the cut is reddish red, and this red pigment is known as a betacyin pigment.

Sasa borealis (Hack.) Makino is an evergreen plant that grows in forests or open spaces below the middle mountains of various places in Korea. It is good for water drop, it grows in semi-shade or sunny place. It is used for ornamental purposes. Fruits are used for edible purposes, leaves and stalks are used for medicinal purposes. It is known that bamboo has the strongest weakness and is effective in treating intractable diseases such as diabetes, hypertension, gastritis, gastric ulcer, chronic hepatitis and cancer even when only one kind of bamboo is used. It is known to lower fever, poison, It is known that it has the effect of treating inflammation and suppressing cancer cells.

The term "extract" as used herein refers to an active ingredient isolated from a natural product, that is, a substance exhibiting a desired activity, and is usually prepared by an extraction process using water, an organic solvent, or a mixed solvent thereof, An extract of a solvent, a dry powder thereof, or any form thereof formulated with the same. In addition, the above-mentioned extract also includes a fraction obtained by fractionating the extract obtained through the above extraction process.

The solvent of the extract of the present invention can be water, an organic solvent or a mixture thereof. Specifically, water, a lower alcohol having 1 to 5 carbon atoms such as methanol and ethanol, a polar solvent such as ethyl acetate and a solvent such as hexane or dichloromethane An organic solvent such as a nonpolar solvent or a mixture thereof, preferably water, a low-alcohol having 1 to 5 carbon atoms or a mixture thereof, more preferably water or ethanol.

The extraction method of the present invention can be performed by methods such as hot water extraction, immersion extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, reflux cooling extraction and ultrasonic extraction.

Dermatological pharmaceutical composition

The present invention provides an external dermatological pharmaceutical composition for preventing hair loss and promoting hair growth, which comprises a mugwort extract, a rhizome extract, and a mugwort extract.

The dermatological pharmaceutical composition may be, but is not limited to, a cream, a gel, a patch, a spray, an ointment, a warning agent, a lotion, a liniment, a pasta or a cataplasma.

The preferred dosage of the pharmaceutical composition will vary depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several doses, but is not limited thereto.

The pharmaceutical composition may contain 1-300 parts by weight of a rhizome extract and 1-300 parts by weight of an extract of Curcuma larvae, based on 100 parts by weight of the extract.

Preferably, 10 to 200 parts by weight of the granular extract and 10 to 200 parts by weight of the granular extract may be contained per 100 parts by weight of the extracts of Munjangran extract, more preferably 20 to 100 parts by weight of the granular extract, May contain 20-100 parts by weight.

In addition, 1 to 200 parts by weight of the extract may be added to 100 parts by weight of the extract, and preferably 5 to 100 parts by weight of the extract may be added to 100 parts by weight of the extract. Preferably 10 to 50 parts by weight, based on 100 parts by weight of the composition.

Furthermore, it may further contain 1 to 200 parts by weight of the Sasa extract, preferably 10 to 100 parts by weight, relative to 100 parts by weight of the Mizuno extract, relative to 100 parts by weight of the Mizuhachi extract. Preferably 10 to 50 parts by weight, based on 100 parts by weight of the composition.

In addition, the green tea extract may be added as an active ingredient of the pharmaceutical composition, and 1-100 parts by weight of the green tea extract may be contained per 100 parts by weight of the extract.

Cosmetic composition

The present invention provides a cosmetic composition for preventing hair loss and promoting hair growth, which comprises a mugwort extract, a rhizome extract, and a mugwort extract.

The cosmetic composition may be at least one selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, Hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, A hair mousse or a hair spray, but is not limited thereto.

The cosmetic composition may comprise a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

As water-soluble vitamins, any of water-soluble vitamins can be used as long as they can be incorporated in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And salts thereof (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate etc.) can also be used as water-soluble vitamins . The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins Any of vitamins A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) , And derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbic acid, dipalmitic acid ascorbic acid, dl-alpha tocopheryl acetate, dl-alpha tocopherol nicotinic acid vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, Ethyl ether) and the like are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be mentioned. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be compounded in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

Seaweed extract can be any compound that can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red tiger extract, green algae extract, and the like. , Potassium alginate, and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The above-mentioned cosmetics may be blended with other ingredients usually added to cosmetics, if necessary. Examples of the compounding ingredient that can be added include a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, , A blood circulation accelerator, a cold agent, an antiperspirant agent, and purified water.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

The cosmetic composition may take the form of a solution, an emulsion, a viscous mixture or the like, and may contain customary adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and fragrances commonly used in cosmetic compositions.

The cosmetic composition may be prepared in any form conventionally produced in the art, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences, hair cosmetics and the like.

The cosmetic composition of the present invention may contain 1-300 parts by weight of Rhizome extract and 1-300 parts by weight of Lauroginae japonica extract, based on 100 parts by weight of the extract.

Preferably, 10 to 200 parts by weight of the granular extract and 10 to 200 parts by weight of the granular extract may be contained per 100 parts by weight of the extracts of Munjangran extract, more preferably 20 to 100 parts by weight of the granular extract, May contain 20-100 parts by weight.

In addition, 1 to 200 parts by weight of the extract may be added to 100 parts by weight of the extract, and preferably 5 to 100 parts by weight of the extract may be added to 100 parts by weight of the extract. Preferably 10 to 50 parts by weight, based on 100 parts by weight of the composition.

Further, it may further contain 1 to 200 parts by weight of the Sasa extract relative to 100 parts by weight of the Mizuhachi extract, preferably 10 to 100 parts by weight of the Sasa extract, relative to 100 parts by weight of the Mizuho Extract, Preferably 10 to 50 parts by weight, based on 100 parts by weight of the composition.

In addition, the green tea extract may be contained as an active ingredient of the pharmaceutical composition, and 1-100 parts by weight of the green tea extract may be contained per 100 parts by weight of the extract.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples and experimental examples.

< Example >

1. Preparation of mixed composition

In order to prepare the mixed composition, the mugwort extract was purchased as powder from SK bioland, and the dried mugwort, mugwort mugwort, mugwort mugwort, and pawrikwi were purchased as standardized herbal medicine, dipped in DW at 10 times, extracted at 50 ° C for 24 hours, The extract was filtered and concentrated to obtain a solid concentrate.

The mixing ratios of the prepared materials were varied to prepare mixed compositions, and the weight ratios thereof are shown in Table 1.

Weight ratio Moon River water chestnut Shag Baekwikcho Sage Example 1 3 3 3 0.5 0.5 Example 2 2 2 2 2 2 Example 3 4 3 2 0.5 0.5 Example 4 4 2 3 0.5 0.5 Example 5 4 4 One 0.5 0.5 Example 6 4 One 4 0.5 0.5 Example 7 4 2 2 One One Example 8 3.5 3.5 2 0.5 0.5 Example 9 3.5 2 3.5 0.5 0.5 Example 10 3 2 2 1.5 1.5 Example 11 3.3 3.3 3.3 Example 12 2.5 5 2.5

< Experimental Example >

One. Of dermal papilla cells (DP-CELL)  Viability test

(One) Dermal papilla  Prevention of hair loss and promotion of hair growth

Hair growth is caused by a complex mechanism caused by various genes, growth factors, receptors, systemic hormones, and the like. Especially, hair follicular dermal matrix cells consisting of epithelial cells and dermal papillary cells consisting of mesenchymal cells (dermal papilla cell) is known to act as a pivotal factor in the formation and growth of hair.

The dermal papilla cells are the cells located at the base of the hair follicle. They supply oxygen and nutrients to the cells constituting the hair follicle, and control hair growth and follicle cycle regulation. Therefore, when the growth of the dermal papilla cells is promoted, the hair becomes healthy, hair growth is promoted, and hair loss can be prevented. The growth of hair progresses as the epithelial cells surrounding the dermal papilla divide and form the hair shaft, so the dermal papilla cells play an important role in regulating the division of epithelial cells. In addition, in male alopecia, the region where the male hormone acts on the hair follicle also plays a very important role in hair growth.

(2) MTT (3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, a yellow tetrazole)

This experiment is an experimental technique to accurately measure cell proliferation and living cells. The yellow water-soluble MTT is reduced to a purple colored water-insoluble MTT formazan by the dehydrogenase action of mitochondria as shown in the following formula (1), so that living cells and dead cells can be discriminated by color change as shown in FIG.

(3) Preparing the experiment

First, MTT (Thiazolyl blue tetrazolium bromide, amresco), trypsin-EDTA (1x) (0.25%, GenDEPOT), Dulbecco's PBS (GenDEPOT), DMEM (GenDEPOT ), Filter paper (Whatman  qualitative filter paper, Grade 2, 8 탆), a syringe filter (Sartorius stedim Minisart filters, 0.2 탆) and the mixed compositions of the above Examples 1 to 12 were prepared.

Next, the PBS solution and the MTT powder reagent were completely dissolved in a conical tube with a foil-shielded conical tube at a concentration of 5 mg / ml, transferred into a clean bench, and filtered using a syringe filter to prepare an MTT solution Respectively.

(4) Experimental method

First, the culture medium of the dermal papilla cells in the T75 flask was removed, 3 ml of PBS was added, and the surface was shaken evenly. After removing the PBS, 2 ml of trypsin-EDTA was added and incubated in a CO ₂ incubator for 2 minutes. Incubate the cultured cells again in a clean bench, add 8 ml of DMEM medium, and harvest the cells on a 15-ml conical tube at the surface by pipetting. The cells were centrifuged at 800 rpm at 4 ° C for 3 minutes, and then all of the medium was removed, leaving only the cell pellet.

Next, 10 ml of DMEM medium containing 10% FBS (serum) was added to the cell pellet, and the pellet was suspended so as to be uniformly released. The cells were counted and 200 μl of the solution was dispensed into each well so that cells of 1 × 10 ⁴ cells / well were contained in 96 wells and cultured in a CO ₂ incubator for 24 hours. The cultured T75 flask was transferred to a clean bench, and the culture solution was removed so that the cells adhering to the bottom did not fall off. Then, 180 μl of serum-free DMEM medium was added to each well.

The mixed composition prepared in Examples 1 to 12 was prepared at the respective concentrations (0.5%, 0.25%, 0.01%, and 0.005%) by using the third distilled water as a control, and the cells were treated with 20 μl each , And cultured in a CO ₂ incubator for 24 hours.

The cultured cells were transferred to a clean bench and treated with 20 μl of each MTT solution. Each well was blocked with light, and incubated for 4 hours in a CO ₂ incubator. After removing the medium, 100 μl of DMSO was added thereto, orbital shaker), and the plate was wrapped with foil to prevent light from entering.

The absorbance of 570 nm (peak absorption of formazan) was measured using a microplate reader, and the cell viability was confirmed by measuring the absorbance of the formazan peak as a percentage of the control value.

(5) Experimental results

The cell viability results of Examples 1 to 12 are shown in Table 2.

As shown, all of the examples showed better viability than the control, especially when the 0.025% concentration of the mixture of Example 6 was treated, the viability was increased by about 185% or more as compared with the control.

Thus, it was confirmed that the mixed compositions of the above embodiments can enhance the survival ability of the dermal papilla cells, thereby preventing hair loss and promoting hair growth.

Cell viability (%) Treatment concentration (%) 0.05 0.025 0.01 0.005 Example 1 111.7794 123.4774 126.9674 158.2268 Example 2 105.9115 115.8476 123.5147 125.8613 Example 3 141.8233 132.2118 145.9649 136.7857 Example 4 111.9406 117.6732 127.7791 178.9581 Example 5 151.6647 131.472 147.872 173.0372 Example 6 149.1076 185.126 145.5689 140.1958 Example 7 130.6563 149.6313 149.871 161.46 Example 8 158.6311 150.858 151.9913 139.4161 Example 9 131.8279 125.2736 126.6574 128.2488 Example 10 115.0948 122.7626 119.4523 132.1156 Example 11 141.8539 149.3089 155.4274 132.5057 Example 12 123.598 131.5506 135.6885 137.881

Claims (8)

A composition for external application for skin for preventing hair loss and promoting hair growth, which comprises a mugwort extract, a rhizome extract, and a mugwort extract.
The method according to claim 1,
Wherein the extract comprises 1-300 parts by weight of the extract and 100-700 parts by weight of the extract of Artemisia princeps.
The method according to claim 1,
Wherein the composition further comprises 1 to 200 parts by weight of the extract of Paeoniae radix with respect to 100 parts by weight of the extract.
The method according to claim 1,
Wherein the composition further comprises 1 to 200 parts by weight of a Sasa extract, based on 100 parts by weight of the extract.
A cosmetic composition for preventing hair loss and promoting hair growth, comprising a mugwort extract, a mulberry extract, and a mugwort extract.
6. The method of claim 5,
The cosmetic composition according to claim 1, wherein 1-300 parts by weight of the wilt extract and 1-300 parts by weight of the rape wormwood extract are contained in 100 parts by weight of the extract.
6. The method of claim 5,
Wherein the cosmetic composition further comprises 1 to 200 parts by weight of the extract of Paeoniae radix against 100 parts by weight of the extract.
The method according to claim 1,
Wherein the cosmetic composition further comprises 1 to 200 parts by weight of a sage extract on the basis of 100 parts by weight of the extract.

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