KR101860960B1 - 다능성 인간 줄기세포의 증식을 위한 조성물 및 방법 - Google Patents

다능성 인간 줄기세포의 증식을 위한 조성물 및 방법 Download PDF

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KR101860960B1
KR101860960B1 KR1020127026894A KR20127026894A KR101860960B1 KR 101860960 B1 KR101860960 B1 KR 101860960B1 KR 1020127026894 A KR1020127026894 A KR 1020127026894A KR 20127026894 A KR20127026894 A KR 20127026894A KR 101860960 B1 KR101860960 B1 KR 101860960B1
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cells
stem cells
human
embryonic stem
laminin
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KR20120138810A (ko
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칼 트리그베이슨
세르게이 로딘
안나 도모가츠카야
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세르게이 로딘
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Genetics & Genomics (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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KR1020127026894A 2010-03-17 2011-03-17 다능성 인간 줄기세포의 증식을 위한 조성물 및 방법 Active KR101860960B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/725,877 US10829733B2 (en) 2007-01-04 2010-03-17 Composition and method for enabling proliferation of pluripotent human stem cells
US12/725,877 2010-03-17
PCT/IB2011/000982 WO2011114237A2 (en) 2010-03-17 2011-03-17 Composition and method for enabling proliferation of pluripotent human stem cells

Publications (2)

Publication Number Publication Date
KR20120138810A KR20120138810A (ko) 2012-12-26
KR101860960B1 true KR101860960B1 (ko) 2018-05-24

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KR1020127026894A Active KR101860960B1 (ko) 2010-03-17 2011-03-17 다능성 인간 줄기세포의 증식을 위한 조성물 및 방법

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US (1) US10829733B2 (enExample)
JP (1) JP6016642B2 (enExample)
KR (1) KR101860960B1 (enExample)
WO (1) WO2011114237A2 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011026221A1 (en) * 2009-09-01 2011-03-10 Mcmaster University Methods of culturing embryoid bodies
JP5590646B2 (ja) 2009-10-08 2014-09-17 国立大学法人大阪大学 ヒト多能性幹細胞用培養基材およびその利用
DK2499236T3 (da) 2009-11-12 2020-03-23 Technion Res & Dev Foundation Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand
KR101966033B1 (ko) 2010-12-17 2019-04-04 바이오라미나 에이비 재조합 라미닌-521
CA2849244C (en) 2011-09-22 2023-10-10 Karl Tryggvason Cell culture substrate comprising a laminin and a cadherin
US9828630B2 (en) 2011-11-30 2017-11-28 Biolamina Ab Methods of enhancing genetic diagnosis
MX354995B (es) 2011-12-05 2018-03-27 Factor Bioscience Inc Metodos y productos para transfeccion de celulas.
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
US9351914B2 (en) 2012-03-25 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for modulating hair growth using truncated laminin-511
KR102315098B1 (ko) 2012-11-01 2021-10-21 팩터 바이오사이언스 인크. 세포에서 단백질을 발현시키는 방법들과 생성물들
RS57257B1 (sr) 2012-12-03 2018-08-31 Biolamina Ab Metodi proizvodnje rpe ćelija
RU2714404C2 (ru) 2014-01-31 2020-02-14 Фэктор Байосайенс Инк. Способы и продукты для получения и доставки нуклеиновых кислот
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
US10576167B2 (en) 2016-08-17 2020-03-03 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP4235175A3 (en) 2017-05-05 2023-10-11 National University of Singapore Methods for culturing human keratinocytes
CN114450265B (zh) 2019-07-03 2024-12-24 菲克特生物科学股份有限公司 阳离子脂质及其用途
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods

Family Cites Families (6)

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Publication number Priority date Publication date Assignee Title
US5728553A (en) * 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
EP1177291A2 (en) 1999-04-30 2002-02-06 Biostratum, Inc. Laminin 8 and methods for its use
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6933273B2 (en) 2000-12-21 2005-08-23 Karl Tryggvason Isolated laminin 10
EP2137295B1 (en) 2007-01-04 2018-04-18 Karl Tryggvason Use of a composition and method for enabling proliferation of pluripotent stem cells
WO2011110886A1 (en) 2010-03-09 2011-09-15 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nat Biotechnol. 2006 Feb;24(2):185-7
Nat Methods. 2006 Aug;3(8):637-46.

Also Published As

Publication number Publication date
WO2011114237A2 (en) 2011-09-22
WO2011114237A3 (en) 2012-01-05
US10829733B2 (en) 2020-11-10
US20100203635A1 (en) 2010-08-12
JP2013521795A (ja) 2013-06-13
KR20120138810A (ko) 2012-12-26
JP6016642B2 (ja) 2016-10-26

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