KR101856927B1 - 외래 유전자 발현 증강 활성을 갖는 dna 요소 - Google Patents

외래 유전자 발현 증강 활성을 갖는 dna 요소 Download PDF

Info

Publication number
KR101856927B1
KR101856927B1 KR1020187005003A KR20187005003A KR101856927B1 KR 101856927 B1 KR101856927 B1 KR 101856927B1 KR 1020187005003 A KR1020187005003 A KR 1020187005003A KR 20187005003 A KR20187005003 A KR 20187005003A KR 101856927 B1 KR101856927 B1 KR 101856927B1
Authority
KR
South Korea
Prior art keywords
foreign gene
polynucleotide
seq
sequence
polynucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020187005003A
Other languages
English (en)
Korean (ko)
Other versions
KR20180021246A (ko
Inventor
다이스케 니시미야
다츠야 이노우에
Original Assignee
다이이찌 산쿄 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 다이이찌 산쿄 가부시키가이샤 filed Critical 다이이찌 산쿄 가부시키가이샤
Publication of KR20180021246A publication Critical patent/KR20180021246A/ko
Application granted granted Critical
Publication of KR101856927B1 publication Critical patent/KR101856927B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51Complete heavy chain or Fd fragment, i.e. VH + CH1
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515Complete light chain, i.e. VL + CL
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/106Plasmid DNA for vertebrates
    • C12N2800/107Plasmid DNA for vertebrates for mammalian
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/46Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020187005003A 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소 Active KR101856927B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2010-154782 2010-07-07
JP2010154782 2010-07-07
PCT/JP2011/065916 WO2012005378A2 (en) 2010-07-07 2011-07-06 Dna element having the activity of enhancing foreign gene expression

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020137000305A Division KR101833570B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소

Publications (2)

Publication Number Publication Date
KR20180021246A KR20180021246A (ko) 2018-02-28
KR101856927B1 true KR101856927B1 (ko) 2018-05-10

Family

ID=44630215

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020187005003A Active KR101856927B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소
KR1020137000305A Active KR101833570B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소
KR1020187005004A Active KR101920187B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020137000305A Active KR101833570B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소
KR1020187005004A Active KR101920187B1 (ko) 2010-07-07 2011-07-06 외래 유전자 발현 증강 활성을 갖는 dna 요소

Country Status (14)

Country Link
US (3) US9371543B2 (enExample)
EP (3) EP3026117B1 (enExample)
JP (2) JP5859988B2 (enExample)
KR (3) KR101856927B1 (enExample)
CN (3) CN105950621B (enExample)
AU (3) AU2011274864B2 (enExample)
BR (1) BR112012033780A2 (enExample)
CA (2) CA2804381C (enExample)
ES (3) ES2564227T3 (enExample)
IL (1) IL243763A0 (enExample)
RU (1) RU2013104989A (enExample)
SG (4) SG10201505354PA (enExample)
TW (2) TWI507523B (enExample)
WO (1) WO2012005378A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2564227T3 (es) * 2010-07-07 2016-03-21 Daiichi Sankyo Company, Limited Elemento de ADN que tiene actividad de aumento de expresión de un gen exógeno
RU2014121383A (ru) 2011-11-28 2016-01-27 Дайити Санкио Компани, Лимитед Происходящий из гена человека промотор
CN109790539A (zh) * 2016-10-03 2019-05-21 第一三共株式会社 Hspa5基因的启动子
CN107881174B (zh) * 2017-11-22 2022-06-03 张宝会 一种翻译水平基因表达调控的方法及应用
AU2020447192A1 (en) * 2020-05-11 2023-01-19 Samsung Bioepis Co., Ltd. Human genome-derived polynucleotide and method for producing polypeptide of interest using same
CN119013400A (zh) 2022-04-26 2024-11-22 第一三共株式会社 Eno1基因的启动子

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341251C (en) * 1988-02-12 2001-06-12 David Bernard Boyle Pox virus vectors
US20070161003A1 (en) * 2003-09-29 2007-07-12 Morris David W Novel therapeutic targets in cancer
EP2105742A1 (en) * 2008-03-26 2009-09-30 Sanofi-Aventis Use of cathepsin C
JP5344682B2 (ja) * 2008-12-26 2013-11-20 グローブライド株式会社 釣糸ガイド、及び釣糸ガイドの製造方法
ES2564227T3 (es) * 2010-07-07 2016-03-21 Daiichi Sankyo Company, Limited Elemento de ADN que tiene actividad de aumento de expresión de un gen exógeno
RU2014121383A (ru) * 2011-11-28 2016-01-27 Дайити Санкио Компани, Лимитед Происходящий из гена человека промотор

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Homo sapiens BAC clone RP11-152F13 from 15, GenBank: AC010724.6
Nat Methods. 2007 Sep;4(9):747-53.

Also Published As

Publication number Publication date
ES2565428T3 (es) 2016-04-04
RU2013104989A (ru) 2014-08-20
BR112012033780A2 (pt) 2016-05-31
EP2591113A2 (en) 2013-05-15
TW201604280A (zh) 2016-02-01
US20190345515A1 (en) 2019-11-14
JP6039048B2 (ja) 2016-12-07
TWI507523B (zh) 2015-11-11
EP2684961A1 (en) 2014-01-15
JP2013531967A (ja) 2013-08-15
ES2564227T3 (es) 2016-03-21
JP5859988B2 (ja) 2016-02-16
CA2804381C (en) 2016-03-22
JP2016054741A (ja) 2016-04-21
CN103080321A (zh) 2013-05-01
US20130171694A1 (en) 2013-07-04
CA2916937A1 (en) 2012-01-12
SG10201505354PA (en) 2015-08-28
AU2018200686B2 (en) 2020-02-13
CN105950621A (zh) 2016-09-21
KR101833570B1 (ko) 2018-03-02
EP2684961B1 (en) 2016-01-20
ES2701091T3 (es) 2019-02-20
CN103865931A (zh) 2014-06-18
US20160333371A1 (en) 2016-11-17
US9371543B2 (en) 2016-06-21
AU2016203681B2 (en) 2018-04-19
EP3026117B1 (en) 2018-09-12
AU2018200686A1 (en) 2018-02-22
AU2011274864B2 (en) 2016-10-06
TW201207103A (en) 2012-02-16
US11060108B2 (en) 2021-07-13
AU2016203681A1 (en) 2016-06-23
IL243763A0 (en) 2016-04-21
KR101920187B1 (ko) 2018-11-19
KR20180021247A (ko) 2018-02-28
KR20180021246A (ko) 2018-02-28
WO2012005378A2 (en) 2012-01-12
EP2591113B1 (en) 2016-01-20
WO2012005378A3 (en) 2012-04-12
SG186788A1 (en) 2013-02-28
EP3026117A1 (en) 2016-06-01
AU2011274864A1 (en) 2013-02-07
KR20130111514A (ko) 2013-10-10
SG2014009187A (en) 2014-04-28
SG10201705118RA (en) 2017-07-28
CA2804381A1 (en) 2012-01-12
US10407694B2 (en) 2019-09-10
CN105950621B (zh) 2019-07-30
CN103865931B (zh) 2018-10-02

Similar Documents

Publication Publication Date Title
AU2019250224B2 (en) Enhanced transgene expression and processing
AU2016203681B2 (en) DNA element having the activity of enhancing foreign gene expression
AU2019360182B2 (en) Methods for targeted insertion of DNA in genes
AU2018315428B2 (en) Integration sites in CHO cells
KR101476010B1 (ko) 고생산성 세포의 수립을 위한 발현 벡터 및 고생산성 세포
CN104066839B (zh) 衍生自人基因的启动子
AU2018204332A1 (en) Expression cassette
KR102553990B1 (ko) Hspa5 유전자의 프로모터
KR20210042084A (ko) Hspa8 유전자의 프로모터
CN113473850A (zh) 具有遗传修饰的钠通道的啮齿动物及其使用方法
AU2023258798A1 (en) PROMOTER OF Eno1 GENE

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20180220

Application number text: 1020137000305

Filing date: 20130104

PA0201 Request for examination
PG1501 Laying open of application
E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20180330

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20180503

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20180503

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20210420

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20220418

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20240408

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 20250407

Start annual number: 8

End annual number: 8