KR101838177B1 - Composition for preventing or treating of Th2-mediated immune disease comprising enzyme-treated extracts from Piperis Nigri Fructus as an active ingredients - Google Patents
Composition for preventing or treating of Th2-mediated immune disease comprising enzyme-treated extracts from Piperis Nigri Fructus as an active ingredients Download PDFInfo
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- KR101838177B1 KR101838177B1 KR1020150174585A KR20150174585A KR101838177B1 KR 101838177 B1 KR101838177 B1 KR 101838177B1 KR 1020150174585 A KR1020150174585 A KR 1020150174585A KR 20150174585 A KR20150174585 A KR 20150174585A KR 101838177 B1 KR101838177 B1 KR 101838177B1
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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Abstract
The present invention relates to a composition for preventing or treating a Th2-mediated immune disease, comprising an enzyme-treated pepper extract as an active ingredient. The inventors of the present invention found that the splenocytes treated with the enzyme-treated pepper extract significantly enhanced the inhibitory activity against IL-4 production compared to the untreated pepper extracts by immunizing mice with the OVA-immunized allergen, As a result of confirming the inhibitory activity of the Th2-mediated immune response, the composition according to the present invention can be effectively used for the development of therapeutic agents, health functional foods, cosmetics, etc. for the prevention, improvement or treatment of Th2- It is expected to be.
Description
The present invention relates to a composition for preventing or treating a Th2-mediated immune disease, comprising an enzyme-treated pepper extract as an active ingredient.
The immune balance regulated by cytokines produced by Th1 (type 1 helper T) and Th2 (type 2 helper T) cells is called the Th1 / Th2 balance. Th1 cells are important for the regulation of cellular immunity, It plays an important role in humoral regulation. Th1 / Th2 balance is maintained constantly by interfering with cytokine mainly on IFN-γ and cytokine mainly on IL-4, which are important for differentiation of Th2, If broken, various immune diseases can be induced. For example, in the case of deflecting Thl, cellular immunity is resurfaced to enhance infection resistance, and when deflected to Th2, infection resistance is reduced, and conversely, allergy is enhanced.
Allergy is a disease caused by a malfunction of the immune system, which causes irritation such as urticaria, itching, runny nose, and cough in a specific person. It is caused by environmental pollution, westernization of diet and lifestyle, , ≪ / RTI > and various allergens. About 20% to 25% of the world's population is exposed to allergic diseases, and the prevalence is on a steady rise.
Allergic reactions are traditionally divided into four classes based on the time and type of expression: type I-type III is a humoral immune response (immediate type) involving antibodies, type IV is a cell-mediated immune response Delay type). Most allergic reactions are type I, and are typical diseases such as asthma, rhinitis, conjunctivitis, food and drug allergy, and atopic dermatitis. In severe cases, anaphylaxis may occur.
The type I immediate-type hypersensitivity reaction is divided into two stages. In the first step, the Th1 cell reaction which produces IL-12 and IFN-γ which suppresses the secretion of IgE and IgG1 by the invasion of the allergen and increases the secretion of IgG2a And Th2 (type 2 helper T) cell responses that produce IL-4, IL-5 and IL-13 are tilted toward Th2 and IL-4 and IL-13 are secreted by excessive immune response of Th2. It is said that IgE-specific antibodies produced by B cells are attached to the surface of mast cells or basophils, and allergens are sensitized to allergens.
The second stage of the allergic manifestation is divided into an initial reaction and a late reaction. The initial reaction re-enters the body to stimulate mast cells and triggers the degranulation reaction. At this time, the vasodilation by histamine, lipid metabolites, cytokines, And late response is activated by infiltration of neutrophils, eosinophils, macrophages, Th2 cells, basophils, and the like in the tissues, thereby causing inflammation and causing atopic dermatitis, rhinitis and asthma. Among these degranulation secretions, histamine is the most well-known factor and is also used as an important indicator of allergic symptoms in relation to immediate hypersensitivity reaction.
Allergy remedies have been the mainstream in the 21st century and have been actively researched. The global market of about 26 trillion won has been formed, and the health functional food market is expected to grow to about 10% by 6.5 trillion won. Approximately 50 million people in the US have diverse allergic diseases and about $ 5.2 billion in the drug market. In Korea, the market for anti-allergic drugs is steadily increasing to KRW440bn and the health functional food market to KRW145bn.
However, most of the drugs currently used for the treatment of allergy are focused on the suppression of the secretion of histamine secreted in the last stage of the allergy mechanism, or the treatment of the symptoms caused by allergy. The treatment of allergic diseases can be done for a long period of time. Most of the drugs with excellent therapeutic effect are widely used for atopic dermatitis, allergic rhinitis and asthma as long as they are steroid drugs. However, long term use can cause serious side effects. Antihistamines also cause symptoms to feel alleviated, but long-term use causes side effects such as depression, concentration difficulties, lethargy, drowsiness, and hepatic disorders, and the problem of recurrence of allergic diseases is discontinued. Furthermore, since allergic diseases, especially atopic dermatitis, are mostly in children and adolescents, it is very important to develop a safe therapeutic agent free from toxicity and side effects, and researches to overcome these limitations are actively conducted. However, It is not easy. Accordingly, there is a growing need for the development of safe functional foods which can reduce the dose of these drugs and replace them to some extent, and have no side effects for long-term treatment.
On the other hand, pepper ( Piperis Nigri Fructus) is the fruit of evergreen plant of the dicotyledonous plant pepper tree pepper and is a natural product familiar with spice. It is widely produced mainly in Southeast Asia and widely distributed in India, Indonesia and Malay Peninsula including Korea. Pepper is not only widely used as a spice, it is said to have the effect of warming the body, removing wind and cold, and eliminating the poison of all fish, meat and mushrooms. In India, it has been used as a traditional medicament for diseases such as bronchitis, gastrointestinal diseases and arthritis, and it has been reported through various studies that it is effective for diseases such as anti-inflammation, pain relief, anti-cancer effect, diabetic complication inhibition, Patent No. 2014-0064067). In addition, it is added to increase the efficacy of other nutrients such as curcumin, saponin and the like because it suppresses the removal of nutrients from cells by controlling the enzyme. Furthermore, studies have been made to utilize pepper for the prevention or treatment of allergic diseases. However, there is a limit to the antiallergic effect of the extract of pepper extracted by the general solvent extraction method. Therefore, studies for improving the efficacy or obtaining new effects are needed.
Accordingly, the present inventors have studied the effect of enhancing the antiallergic activity after treating the pepper extract with an enzyme, and completed the present invention on the basis thereof.
The inventors of the present invention have conducted intensive studies to improve the preventive or therapeutic effect of diseases caused by the Th2 immune response such as allergy to pepper extract. As a result, it has been found that the extract of pepper treated with the enzyme is more effective than the enzyme- The inhibitory activity is markedly improved, thereby completing the present invention.
Accordingly, it is an object of the present invention to provide a composition for preventing, improving, or treating a Th2-mediated immune disease, which comprises an enzyme-treated pepper extract as an active ingredient.
However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
In order to achieve the object of the present invention, the present invention provides a pharmaceutical composition for preventing or treating Th2-mediated immune diseases, which comprises an extract of Piperis Nigri Fructus as an active ingredient.
In one embodiment of the present invention, the Th2-mediated immune disease may be an allergic disease.
In another embodiment of the present invention, the enzyme may be beta-glucosidase.
In another embodiment of the present invention, the extract is selected from the group consisting of water, C 1 to C 4 lower alcohols, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, ≪ / RTI >
In another embodiment of the present invention, the composition may inhibit the production of IL-4.
The present invention is enzyme-treated pepper (Piperis Nigri Fructus) as an active ingredient. The present invention also provides a health functional food composition for preventing or ameliorating a Th2-mediated immune disease.
The present invention is enzyme-treated pepper (Piperis Nigri Fructus) as an active ingredient. The present invention also provides a cosmetic composition for preventing or ameliorating a Th2-mediated immune disease.
The present invention also provides a method of preventing or treating a Th2-mediated immune disease, comprising administering the composition to a subject.
The present invention also provides the use of the composition for the prevention or treatment of a Th2-mediated immune disorder.
The inventors of the present invention found that the splenocytes treated with the enzyme-treated pepper extract significantly enhanced the inhibitory activity against IL-4 production compared to the untreated pepper extracts by immunizing mice with the OVA-immunized allergen, As a result of confirming the inhibitory activity of the Th2-mediated immune response, the composition according to the present invention can be effectively used for the development of therapeutic agents, health functional foods, cosmetics, etc. for the prevention, improvement or treatment of Th2- It is expected to be.
Figure 1 shows the results of treatment of mouse spleen cells immunized with OVA at different concentrations (6.25, 12.5, 25, or 50 占 퐂 / ml) of each of the pepper extracts treated with or without beta-glucosidase, After culturing, the amount of IL-4 from the culture was measured by ELISA.
The present invention relates to a pepper extract having enhanced activity through enzyme treatment and its use.
Thus, the present invention is enzyme-treated pepper (Piperis Nigri Fructus) as an active ingredient. The present invention also provides a pharmaceutical composition for preventing or treating a Th2-mediated immune disease.
As used herein, the term " prophylactic " means any action that inhibits or delays the development of a Th2-mediated immune disorder by administration of a pharmaceutical composition according to the present invention.
As used herein, the term " treatment " means any action that improves or alters the symptoms of a Th2-mediated immune disorder by administering the pharmaceutical composition of the present invention.
"Pepper", a natural material used in the present invention, is a natural product of spice, which is a fruit of evergreen tree plant of the dicotyledonous plant pepper tree pepper. It is generally known to be widely used as a spice, as well as to warm the body, remove wind and cold, and eliminate poisons from all fish, meat and mushrooms. In India, it has been used as a traditional medicine for diseases such as bronchitis, gastrointestinal diseases and arthritis. It has been reported through various studies that it is effective for diseases such as anti-inflammation, pain relief, anti-cancer effect, diabetic complication suppression and vitiligo. In addition, it is added to increase the efficacy of other nutrients such as curcumin, saponin and the like because it suppresses the removal of nutrients from cells by controlling the enzyme. In the present invention, pepper can be used as an extracting raw material without any limitation in its form, and powdery form can be used.
The term " Th2-mediated immune disease ", which is a disease to be treated in the present invention, refers to a disease in which IgE and mast cells are involved by the production and activation of allergens, particularly Th2 cells. Examples include allergies, Skin diseases including dermatitis, acute and chronic allergic rhinitis, asthma, food or drug allergy, and the like.
The term " enzyme " used in the present invention means a polymeric organic catalyst produced by living body cells, which serves as a catalyst for a chemical reaction in a living organism. There are various kinds of enzymes in the cells of all living things such as animals and plants, and they are involved in the chemical reaction of living things. Enzymes are very complex because their chemical structures are very complex. They are classified according to the kind of substrate and reaction type. Depending on the type of reaction, hydrolase, transferase, lyase and synthetase, It is classified as isomerase. Enzymes are used for a variety of purposes such as pharmaceuticals, large-scale use of starch saccharification by amylase, cheese production, juice purification, textile industry, synthesis of vitamins and amino acids. In the present invention, beta-glucosidase was used to enhance the activity of pepper. The β-glucosidase is an enzyme that acts on β1-> 4 bonds connecting two glucose or glucose-substituted molecules. The β-glucosidase is a cellulose or related polysaccharide Cellulase, more specifically various exocellulases having various beta-D-glucoside substrate specificities. The term " cellulase "
The enzyme-treated pepper extract of the present invention comprises the steps of: obtaining a pepper extract; And adding the enzyme to the pepper extract and reacting them. Specifically, the step of adding and reacting the enzyme may be performed at 37 DEG C for 15 to 40 hours, more preferably 20 to 30 hours.
The pepper extract can be extracted using a conventional solvent known in the art for extracting an extract from a natural product, that is, under ordinary temperature and pressure conditions. For example, in the present invention, the pepper extract may be at least one selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, Can be extracted using a solvent, and preferably ethanol can be used for extraction. In addition, the method of extracting the black pepper extract may be carried out by various methods such as room temperature extraction, hot water extraction, cold extraction, reflux extraction, microwave extraction, and ultrasonic extraction. This is not restrictive.
The extract thus prepared may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. For example, the filtration can be performed using a filter paper or a vacuum filter, the concentration can be carried out using a vacuum concentrator, and the lyophilization can be carried out, but the present invention is not limited thereto.
Further, the extract extracted with the solvent may be further fractionated with a solvent selected from the group consisting of butanol, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, and a mixed solvent thereof. The fractionation temperature may be 4 캜 to 120 캜, but is not limited thereto.
In one embodiment of the present invention, the following experiment was conducted to confirm the improvement or therapeutic effect of Th2-mediated immune disease on the enzyme-treated pepper extract.
In one embodiment of the present invention, splenocytes isolated from OVA-immunized mice were treated with OVA, untreated pepper extract or enzyme-treated pepper extract at various concentrations, and the cells were cultured for enzyme treatment , The inhibitory activity of the extract of pepper on IL-4 was remarkably enhanced (see Example 2).
Therefore, it was confirmed that the composition of the present invention can inhibit the production of IL-4.
The pharmaceutical composition according to the present invention contains an enzyme-treated pepper extract as an active ingredient, and may further comprise a pharmaceutically acceptable carrier. Such pharmaceutically acceptable carriers are those conventionally used in the field of application and include, but are not limited to, saline, sterile water, Ringer's solution, buffered saline, cyclodextrin, dextrose solution, maltodextrin solution, glycerol, ethanol, And may further contain other conventional additives such as antioxidants and buffers as needed. In addition, it can be formulated into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like by additionally adding diluents, dispersants, surfactants, binders, lubricants and the like. Suitable pharmaceutically acceptable carriers and formulations can be suitably formulated according to the respective ingredients using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA. The pharmaceutical composition of the present invention is not particularly limited to a formulation, but may be formulated into injections, inhalants, external skin preparations, and the like.
The pharmaceutical composition of the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the intended method, and the dose may vary depending on the condition and the weight of the patient, The mode of administration, the route of administration, and the time, but may be appropriately selected by those skilled in the art.
The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, " pharmaceutically effective amount " means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment. The effective dose level is determined depending on the type of disease, severity, The time of administration, the route of administration and the rate of excretion, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition according to the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, sequentially or concurrently with conventional therapeutic agents, and may be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.
Specifically, the effective amount of the pharmaceutical composition of the present invention may vary depending on the age, sex, condition, body weight, the degree of absorption of the active ingredient in the body, the rate of inactivation and the excretion rate, the type of disease, 0.001 to 150 mg, preferably 0.01 to 100 mg per kg of body weight, may be administered daily or every other day, or one to three divided doses per day. However, the dosage may be varied depending on the route of administration, the severity of obesity, sex, weight, age, etc. Therefore, the dosage is not limited to the scope of the present invention by any means.
In another aspect of the invention there is provided enzyme-treated pepper (Piperis Nigri Fructus) as an active ingredient. The present invention provides a health functional food composition for preventing or ameliorating a Th2-mediated immune disease.
As used herein, the term "improvement" means all actions that at least reduce the degree of symptom associated with the condition being treated. Herein, the health functional food composition may be used simultaneously or separately with the medicament for treatment before or after the onset of the disease for the prevention or improvement of the Th2-mediated immune disease.
The term " health functional food composition " as used in the present invention means a food composition comprising at least one of a carrier, diluent, excipient, and additives, selected from the group consisting of tablets, pills, powders, granules, powders, capsules, Which is characterized in that it is formulated into one. Examples of foods that can be added to the extract of the present invention include various foods, powders, granules, tablets, capsules, syrups, drinks, gums, tea, vitamin complexes, and health functional foods. Examples of the additive that can be further included in the present invention include natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors and the like), colorants, fillers, At least one component selected from the group consisting of alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonating agents and fats can be used. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. As the above-mentioned flavors, natural flavors (tautatin, stevia extract (for example, rebaudioside A and glycyrrhizin) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used. The composition according to the present invention can be used in various forms such as flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies, factic acid and its salts, alginic acid and its salts, , pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, etc. Other compositions according to the present invention may contain flesh for the production of natural fruit juices and vegetable drinks . These components may be used independently or in combination. Specific examples of the carrier, excipient, diluent, and additive include, but are not limited to, lactose, dextrose But are not limited to, sucrose, sorbitol, mannitol, erythritol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium phosphate, calcium silicate, microcrystalline cellulose, polyvinylquilolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water , At least one selected from the group consisting of sugar syrup, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil is preferably used.
As a further aspect of the invention there is provided enzyme-treated pepper (Piperis Nigri Fructus) as an active ingredient. The present invention also provides a cosmetic composition for preventing or ameliorating a Th2-mediated immune disease.
The cosmetic composition of the present invention may contain not only an enzyme-treated pepper extract but also components commonly used in cosmetic compositions, and may contain conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes, And may include a carrier.
In addition to the enzyme-treated pepper extract, the composition of the present invention may be mixed with an organic UV-blocking agent which has been used in the past so long as it does not impair the skin protecting effect by reacting with the enzyme-treated pepper extract. Examples of the organic UV blocking agent include glyceryl paraben, drometrizol trisiloxane, drometrizol, dipaloyyl triolate, disodium phenyldibenzimidazole tetrasulfonate, diethylhexylbutamidotriazone, diethylamino Hydroxybenzoylhexyl benzoate, di-methoxycinnamate, a mixture of Rawson and dihydroxyacetone, methylene bis-benzotriazolyltetramethylbutylphenol, 4-methylbenzylidene camphor, menthyl anthranylate, benzophenone (Benzophenone-4), benzophenone-8 (dioxyphenylbenzone), butylmethoxydibenzoylmethane, bisethylhexyloxyphenol methoxyphenyltriazine, synoxate, ethyl dihydroxypropylparaben, Ethylhexyldimethylpivalate, ethylhexylmethoxycinnamate, ethylhexylsalicylate, ethylhexyltriazone, isoamyl-p-methoxy cinnamate, polysilicon-15 (dimethicodimethylbenzalmate, At least one selected from the group consisting of terephthalylidene dicam peresophilic acid and its salts, thiai-salicylate and aminobenzoic acid (parabe) can be used.
Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, . Specific formulations of the cosmetic composition of the present invention include skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisturizing lotions, nutritional lotions, massage creams, nutritional creams, moisturizing creams, hand creams, essences, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow and the like.
According to a preferred embodiment of the present invention, the content of the enzyme-treated pepper extract of the present invention is 0.00001-30 wt%, preferably 0.5-20 wt%, more preferably 1.0-10 wt%, based on the total weight of the composition . If the content of the enzyme-treated pepper extract is less than 0.00001% by weight, the effect of absorbing ultraviolet light is greatly reduced. If the content is more than 30% by weight, skin irritation may be caused.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.
[ Example ]
Example 1. Preparation and method of experiment
1-1. Manufacture of black pepper extract
1000 g of 70% ethanol was added to 100 g of pepper, and the mixture was subjected to reflux extraction (primary extraction) at 60 ° C for 3 hours. Then, the extract was collected once and 1000 ml of 70% ethanol was further added thereto. (Secondary extraction). The extracts obtained from the first and second extraction were mixed and concentrated under reduced pressure to a final amount of 100 ml using a rotary evaporator. The extracts were used for the experiment while being frozen and freeze - dried.
1-2. β- glucosidase process
In order to treat β-glucosidase in pepper extract, the pepper extract prepared by the method of 1-1 above was dissolved in 50% DMSO (dimethyl sulfoxide) at a concentration of 100 mg / ml, and 0.05 M sodium acetate buffer (pH 5.0) to a concentration of 10 mg / ml. This diluted extract of pepper was treated with 10 units / ㎖ of β-glucosidase and cultured at 37 ℃ for 24 hours.
1-3. Mice immunized with OVA Splenocyte culture
To induce allergy to female BALB / c mice at 5 weeks of age, 10 μg of ovalbumin (OVA) and 1 mg of alum were mixed for 30 minutes, and 100 μl / (N = 5) twice a week. One week after the immunization, the mice were sacrificed by cervical disassembly, and the spleen was removed from the aseptic state. Then, the spleen was transferred to a small petri dish containing 1 ml of the basic medium, i.e., 2-mercaptoethanol and RPMI1640 medium supplemented with antibiotics And stored on ice. The spleen was then single-celled using a mesh, washed twice with 5 ml of basal medium, and then centrifuged at 1500 rpm for 5 minutes. After removing the supernatant, 1 ml of RBC (red blood cell) lysing buffer (SIGMA R7757) was added and incubated for 3 minutes on ice. 10 ml of basal medium was further added, ≪ / RTI > centrifugation twice.
On the other hand, a 96-well plate was treated with OVA as an antigen at a concentration of 100 占 퐂 / ml and treated with? -Glucosidase prepared according to the method of Example 1-1, Pepper extracts were added at concentrations of 6.25, 12.5, 25, and 50 ㎍ / ㎖, respectively. Mouse spleen cells cultured by the above method were treated at 5 × 10 6 cells / well. After incubation in a CO 2 incubator at 37 ° C for 72 hours, the cell culture medium was recovered.
1-4. Using ELISA Splenocyte Cytokine analysis of culture medium
In order to measure the amount of IL-4 cytokine from the mouse spleen cell culture recovered according to the method of Example 1-3, IL-4 BD OptEIATM MOUSE ELISA kit and TGF-β kit (R & D Systems).
Specifically, a capture antibody was added to a 96-well plate and reacted overnight at 4 ° C. The plate was coated with an antibody, washed with a washing buffer (phosphate buffered saline with
Example 2. Th1 / Th2 Cytokine-modulating activity test
The amount of IL-4 cytokine was measured from the culture medium of mouse splenocytes according to the methods of Examples 1-3 and 1-4 in order to evaluate the inhibitory activity of Th2 immune response by the pepper extract treated with? -glucosidase Respectively.
Comparing the inhibitory activity of IL-4 production by each extract, the IC 50 (half maximal inhibitory concentration) inhibiting the production of IL-4 was about 25 ㎍ / ㎖ for the 70% ethanolic pepper extract without enzyme treatment Respectively. On the other hand, the 70% ethanolic pepper extract treated with β-glucosidase showed an IC 50 value of less than 6.25 ㎍ / ㎖. In conclusion, it was confirmed that the inhibition of IL-4 by pepper extract was significantly enhanced by about 4-fold by β-glucosidase treatment.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. There will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.
Claims (7)
Wherein the enzyme is beta-glucosidase.
The extract is characterized by being extracted with a solvent selected from the group consisting of water, C 1 to C 4 lower alcohol, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, Lt; / RTI >
Wherein said composition inhibits the production of IL-4.
Wherein the enzyme is beta-glucosidase.
Wherein the enzyme is beta-glucosidase.
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