KR101838178B1 - Composition for preventing or treating of Th2-mediated immune disease comprising fermented extracts from Piperis Nigri Fructus as an active ingredients - Google Patents
Composition for preventing or treating of Th2-mediated immune disease comprising fermented extracts from Piperis Nigri Fructus as an active ingredients Download PDFInfo
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- KR101838178B1 KR101838178B1 KR1020150174586A KR20150174586A KR101838178B1 KR 101838178 B1 KR101838178 B1 KR 101838178B1 KR 1020150174586 A KR1020150174586 A KR 1020150174586A KR 20150174586 A KR20150174586 A KR 20150174586A KR 101838178 B1 KR101838178 B1 KR 101838178B1
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- extract
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Abstract
The present invention relates to a composition for preventing, ameliorating, or treating a Th2-mediated immune disease, which comprises a pepper fermented extract as an active ingredient. Since the pepper fermented extract according to the present invention exhibits significantly enhanced IL-4 production inhibitory activity as compared to the non-fermented pepper extract, the pepper fermented extract is useful as a therapeutic agent for preventing, improving or treating a Th2- Food, cosmetics, and the like.
Description
The present invention relates to a composition for preventing, ameliorating, or treating a Th2-mediated immune disease, which comprises a pepper fermented extract as an active ingredient.
Allergy is a disease caused by a malfunction of the immune system, which causes irritation such as urticaria, itching, runny nose, and cough in a specific person. It is caused by environmental pollution, westernization of diet and lifestyle, , ≪ / RTI > and various allergens. Twenty to 25 percent of the world's population is exposed to allergic diseases, and the prevalence of this disease continues to rise.
Allergic reactions are traditionally divided into four classes based on the time and type of expression: type I-type III is a humoral immune response (immediate type) involving antibodies, type IV is a cell-mediated immune response Delay type). Most allergic reactions are type I, and are typical diseases such as asthma, rhinitis, conjunctivitis, food and drug allergy, and atopic dermatitis. In severe cases, anaphylaxis may occur.
Type I immediate-type hypersensitivity is divided into two stages. The first stage is IL-12, which suppresses the secretion of IgE and IgG1 by the invasion of allergen, and Th1 (type 1 helper T) cell response and a Th2 (type 2 helper T) cell response that produces IL-4, IL-5 and IL-13 are tilted toward Th2, -13 are secreted, and IgE-specific antibodies produced by B cells are attached to the surface of mast cells or basophils, and allergens are sensitized to allergens.
The second stage of the allergic manifestation is divided into an initial reaction and a late reaction. The initial reaction re-enters the body to stimulate mast cells and triggers the degranulation reaction. At this time, the vasodilation by histamine, lipid metabolites, cytokines, And late response is activated by infiltration of neutrophils, eosinophils, macrophages, Th2 cells, basophils, and the like in the tissues, thereby causing inflammation and causing atopic dermatitis, rhinitis and asthma. Among these degranulation secretions, histamine is the most well-known factor and is also used as an important indicator of allergic symptoms in relation to immediate hypersensitivity reaction.
Allergy remedies have been the mainstream in the 21st century and have been actively researched. The global market of about 26 trillion won has been formed, and the health functional food market is expected to grow to about 10% by 6.5 trillion won. Approximately 50 million people in the US have diverse allergic diseases and about $ 5.2 billion in the drug market. In Korea, the market for anti-allergic drugs is steadily increasing to KRW440bn and the health functional food market to KRW145bn.
However, most of the drugs currently used for the treatment of allergy are focused on the suppression of the secretion of histamine secreted in the last stage of the allergy mechanism, or the treatment of the symptoms caused by allergy. The treatment of allergic diseases can be done for a long period of time. Most of the drugs with excellent therapeutic effect are widely used for atopic dermatitis, allergic rhinitis and asthma as long as they are steroid drugs. However, long term use can cause serious side effects. Antihistamines also cause symptoms to feel alleviated, but long-term use causes side effects such as depression, concentration difficulties, lethargy, drowsiness, and hepatic disorders, and the problem of recurrence of allergic diseases is discontinued. Furthermore, since allergic diseases, especially atopic dermatitis, are mostly in children and adolescents, it is very important to develop a safe therapeutic agent free from toxicity and side effects, and researches to overcome these limitations are actively conducted. However, It is not easy. Accordingly, there is a growing need for the development of safe functional foods which can reduce the dose of these drugs and replace them to some extent, and have no side effects for long-term treatment.
On the other hand, fermentation is a phenomenon in which microorganisms decompose or change organisms with their own enzymes and produce beneficial end products. They have been widely used as human food cooking methods, and one third of human food It is fermented food. Fermented food is considered to be the best health food with its natural taste and aroma. Yogurt, antioxidant and antioxidant wine, and miso and kimchi in Korea are representative. Furthermore, it is known that bacteria that are rich in fermented foods such as yogurt, cheese, miso, etc. prevent allergic diseases. As well as taste, the beneficial effects of fermentation are widely known, and studies have been actively conducted to improve or treat diseases using fermented foods as well as fermented foods.
Specifically, many research results have been reported to improve the original function or to obtain new effects by adding various nutritional ingredients in addition to the original ingredients by fermenting natural products (Korean Patent Laid-Open No. 2013-0125969). For example, the fermented soybean fermented soybean fermented soybeans was recognized as a stabilizing raw material in the phytosanitary hospital after the postprandial glucose control function was suppressed by suppressing the absorption of sugar, and the toxicity reduction and immunity enhancement effect of the fermented extract of lactobacillus of mistletoe were also reported have. In addition to the development of safe natural material itself, there is no side effect for allergic response, and further research is needed to improve the efficacy or obtain new effects by using fermentation.
However, there have been few studies to improve the efficacy by fermenting pepper. Therefore, the present inventors have studied the effect of enhancing the antiallergic activity by preparing pepper fermented extract fermented with pepper, and completed the present invention based on this research.
The inventors of the present invention conducted intensive studies to enhance the antiallergic activity of pepper, and found that the inhibitory activity of IL-4 production in OVA-immunized mouse spleen cells compared with the pepper extracts of the pepper fermented extract prepared through microbial fermentation The present invention has been completed.
Accordingly, it is an object of the present invention to provide a composition for preventing, improving, or treating a Th2-mediated immune disease, which comprises a fermented black pepper extract as an active ingredient.
However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing or treating Th2-mediated immune diseases, comprising a fermented extract of pepper ( Piperis Nigri Fructus) as an active ingredient.
In one embodiment of the present invention, the Th2-mediated immune disease may be an allergic disease.
In another embodiment of the present invention, the fermented extract may be one obtained by mixing pepper and an extraction solvent, and culturing the microorganism for 5 days to 15 days after inoculation.
In another embodiment of the present invention, the extraction solvent comprises water, a C 1 to C 4 lower alcohol, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, Lt; / RTI >
In another embodiment of the present invention, the composition may inhibit the production of IL-4.
The invention also pepper (Piperis Nigri Fructus) fermented extract as an effective ingredient for preventing or ameliorating a Th2-mediated immune disease.
The invention also pepper (Piperis Nigri Fructus) fermented extract as an active ingredient. The present invention also provides a cosmetic composition for preventing or ameliorating a Th2-mediated immune disease.
The present invention also provides a method of preventing or treating a Th2-mediated immune disease, comprising administering the composition to a subject.
The present invention also provides the use of the composition for the prevention or treatment of a Th2-mediated immune disorder.
As a result of treating the spleen cells of the mice immunized with OVA with OVA to induce allergy, the inhibitory activity of IL-4 production was significantly enhanced as compared with the non-fermented pepper extract, indicating that Th2-mediated immunity As a result of confirming the inhibitory activity of the reaction, the pepper fermented extract is expected to be useful for the development of therapeutic agents, health functional foods, and cosmetics for the prevention, improvement, or treatment of Th2-mediated immune diseases.
FIG. 1 is a graph showing the results of immunoprecipitation of OVA-immunized mouse spleen cells with fermented black pepper extract (0 day) and 8 or 12 days of fermented black pepper extract (8 day, 12 day) 4 was measured by ELISA.
The present invention relates to a pepper fermented extract having an enhanced Th2-mediated immunosuppressive activity and its use.
Thus, the present invention pepper (Piperis Nigri Fructus) fermented extract as an active ingredient. The present invention also provides a pharmaceutical composition for preventing or treating a Th2-mediated immune disease.
As used herein, the term " prophylactic " means any action that inhibits or delays the development of a Th2-mediated immune disorder by administration of a pharmaceutical composition according to the present invention.
As used herein, the term " treatment " means any action that improves or alters the symptoms of a Th2-mediated immune disorder by administering the pharmaceutical composition of the present invention.
"Pepper", a natural material used in the present invention, is a natural product of spice, which is a fruit of evergreen tree plant of the dicotyledonous plant pepper tree pepper. It is widely produced mainly in Southeast Asia and widely distributed in India, Indonesia and Malay Peninsula including Korea. Pepper is not only widely used as a spice, it is said to have the effect of warming the body, removing wind and cold, and eliminating the poison of all fish, meat and mushrooms. In India, it has been used as a traditional medicine for diseases such as bronchitis, gastrointestinal diseases and arthritis. It has been reported through various studies that it is effective for diseases such as anti-inflammation, pain relief, anti-cancer effect, diabetic complication suppression and vitiligo. In addition, it is added to increase the efficacy of other nutrients such as curcumin, saponin and the like because it suppresses the removal of nutrients from cells by controlling the enzyme. In the present invention, pepper can be used as a raw material of a fermented extract without any limitation of its form, and a powder form can be used.
As used herein, the term " T cell " refers to a cell that expresses IL-4, IL-5, IL-6, , IL-10, and IL-13. The immune balance regulated by the cytokines produced by these Th1 and Th2 cells is called the Th1 / Th2 balance. Th1 cells are important for the regulation of cellular immunity, and Th2 cells play an important role in controlling humoral immune responses. In the normal state, the cytokine, which is important for the differentiation of Th1, is interfering with the cytokine mainly composed of IFN-γ and IL-4, which is important for differentiation of Th2, and the Thl / Th2 balance is kept constant. However, when the Th1 / Th2 balance is broken, various immune diseases can be induced. In the case of deflecting Thl, the cellular immunity is revived to increase the resistance to infection. When the Th2 is deflected, the infection resistance decreases. Conversely, do.
The term " Th2-mediated immune disease ", which is a disease to which the present invention is directed, means a disease in which IgE and mast cells are involved by the production and activation of allergens, particularly Th2 cells. Examples of such diseases include allergies, Skin diseases including dermatitis and contact dermatitis, acute and chronic allergic rhinitis, asthma, food or drug allergy, and the like.
As used herein, the term " fermentation " means that an organic material is decomposed and changed by microbial action to produce a useful substance. The fermentation may be various depending on the environment. Typically, there are fermentation of alcohol by yeast, fermentation of glycerol, fermentation of lactic acid bacteria with lactic acid, fermentation with lactic acid, methane fermentation with methane bacteria, and mixed organic acid fermentation with Escherichia coli. Oxidative fermentation utilizes the accumulation of intermediate metabolites by incomplete oxidation of the substrate, such as fermentation of acetic acid by acetic acid bacteria, fermentation of gluconic acid, fermentation of sorbose or organic acids such as citric acid, gluconic acid, fumaric acid and oxalic acid by fungi. In the present invention, the pepper fermentation can be carried out for 5 to 15 days, more preferably for 8 to 12 days, by inoculation with Bacillus amyloliquefaciens , but the activity of pepper can be enhanced through fermentation The type of microorganism and fermentation period are not limited.
The pepper fermented extract can be extracted using a conventional solvent known in the art for extracting an extract from a natural product, that is, under ordinary temperature and pressure conditions. For example, in the present invention, the pepper fermented extract may be one selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, The above extraction solvent can be used for extraction, and preferably water can be extracted. In addition, the method of extracting the black pepper fermentation extract can be extracted by various methods such as room temperature extraction, hot water extraction, cold extraction, reflux extraction, microwave extraction, and ultrasonic extraction.
The extract thus prepared may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. For example, the filtration can be performed using a filter paper or a vacuum filter, the concentration can be carried out using a vacuum concentrator, and the lyophilization can be carried out, but the present invention is not limited thereto.
Further, the extract extracted with the solvent may be further fractionated with a solvent selected from the group consisting of butanol, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, and a mixed solvent thereof. The fractionation temperature may be 4 캜 to 120 캜, but is not limited thereto.
In one embodiment of the present invention, in order to confirm the improvement or therapeutic effect of the Th2-mediated immune disease on the pepper fermented extract, OVA, non-fermented pepper extract, 8, or 12 days, respectively. As a result, it was confirmed that the inhibitory activity of IL-4 production in the cell culture liquid was proportional to the fermentation period (see Example 2).
Therefore, it was confirmed that the composition of the present invention can inhibit the production of IL-4.
The pharmaceutical composition according to the present invention may contain a fermented extract of pepper as an active ingredient, and may further comprise a pharmaceutically acceptable carrier. Such pharmaceutically acceptable carriers are those conventionally used in the field of application and include, but are not limited to, saline, sterile water, Ringer's solution, buffered saline, cyclodextrin, dextrose solution, maltodextrin solution, glycerol, ethanol, And may further contain other conventional additives such as antioxidants and buffers as needed. In addition, it can be formulated into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like by additionally adding diluents, dispersants, surfactants, binders, lubricants and the like. Suitable pharmaceutically acceptable carriers and formulations can be suitably formulated according to the respective ingredients using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA. The pharmaceutical composition of the present invention is not particularly limited to a formulation, but may be formulated into injections, inhalants, external skin preparations, and the like.
The pharmaceutical composition of the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the intended method, and the dose may vary depending on the condition and the weight of the patient, The mode of administration, the route of administration, and the time, but may be appropriately selected by those skilled in the art.
The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, " pharmaceutically effective amount " means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment. The effective dose level is determined depending on the type of disease, severity, The time of administration, the route of administration and the rate of excretion, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition according to the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, sequentially or concurrently with conventional therapeutic agents, and may be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.
Specifically, the effective amount of the pharmaceutical composition of the present invention may vary depending on the age, sex, condition, body weight, the degree of absorption of the active ingredient in the body, the rate of inactivation and the excretion rate, the type of disease, 0.001 to 150 mg, preferably 0.01 to 100 mg per kg of body weight, may be administered daily or every other day, or one to three divided doses per day. However, the dosage may be varied depending on the route of administration, the severity of obesity, sex, weight, age, etc. Therefore, the dosage is not limited to the scope of the present invention by any means.
In another aspect of the present invention,Piperis Nigri Fructus) fermented extract as an active ingredient for preventing or ameliorating a Th2-mediated immune disease.
As used herein, the term "improvement" means all actions that at least reduce the degree of symptom associated with the condition being treated. Herein, the health functional food composition may be used simultaneously or separately with the medicament for treatment before or after the onset of the disease for the prevention or improvement of the Th2-mediated immune disease.
The term " health functional food composition " as used in the present invention means a food composition comprising at least one of a carrier, diluent, excipient, and additives, selected from the group consisting of tablets, pills, powders, granules, powders, capsules, Which is characterized in that it is formulated into one. Examples of foods that can be added to the extract of the present invention include various foods, powders, granules, tablets, capsules, syrups, drinks, gums, tea, vitamin complexes, and health functional foods. Examples of the additive that can be further included in the present invention include natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors and the like), colorants, fillers, At least one component selected from alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonating agents and fats can be used. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. As the above-mentioned flavors, natural flavors (tautatin, stevia extract (for example, rebaudioside A and glycyrrhizin) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used. The composition according to the present invention can be used in various forms such as flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies, factic acid and its salts, alginic acid and its salts, , pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, etc. Other compositions according to the present invention may contain flesh for the production of natural fruit juices and vegetable drinks . These components may be used independently or in combination. Specific examples of the carrier, excipient, diluent, and additive include, but are not limited to, lactose, dextrose But are not limited to, sucrose, sorbitol, mannitol, erythritol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium phosphate, calcium silicate, microcrystalline cellulose, polyvinylquilolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water , At least one selected from the group consisting of sugar syrup, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil is preferably used.
In still another aspect, the present invention of the present invention pepper (Piperis Nigri Fructus) fermented extract as an active ingredient. The present invention also provides a cosmetic composition for preventing or ameliorating a Th2-mediated immune disease.
The cosmetic composition of the present invention may contain not only black pepper fermented extract but also conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes, Carrier.
In addition, the composition of the present invention may be used in combination with an organic UV blocking agent which has been used in the past so long as it does not impair the skin protecting effect by reacting with the pepper fermented extract in addition to the peppery fermentation extract. Examples of the organic UV blocking agent include glyceryl paraben, drometrizol trisiloxane, drometrizol, dipaloyyl triolate, disodium phenyldibenzimidazole tetrasulfonate, diethylhexylbutamidotriazone, diethylamino Hydroxybenzoylhexyl benzoate, di-methoxycinnamate, a mixture of Rawson and dihydroxyacetone, methylene bis-benzotriazolyltetramethylbutylphenol, 4-methylbenzylidene camphor, menthyl anthranylate, benzophenone (Benzophenone-4), benzophenone-8 (dioxyphenylbenzone), butylmethoxydibenzoylmethane, bisethylhexyloxyphenol methoxyphenyltriazine, synoxate, ethyl dihydroxypropylparaben, Ethylhexyldimethylpivalate, ethylhexylmethoxycinnamate, ethylhexylsalicylate, ethylhexyltriazone, isoamyl-p-methoxy cinnamate, polysilicon-15 (dimethicodimethylbenzalmate, At least one selected from the group consisting of terephthalylidene dicam peresophilic acid and its salts, thiai-salicylate and aminobenzoic acid (parabe) can be used.
Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, . Specific formulations of the cosmetic composition of the present invention include skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisturizing lotions, nutritional lotions, massage creams, nutritional creams, moisturizing creams, hand creams, essences, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow and the like.
According to a preferred embodiment of the present invention, the content of the peppery fermentation extract of the present invention is 0.00001-30 wt%, preferably 0.5-20 wt%, more preferably 1.0-10 wt%, based on the total weight of the composition. If the content of the peppery fermentation extract is less than 0.00001% by weight, the effect of absorbing ultraviolet light is greatly reduced. If the content is more than 30% by weight, skin irritation may be caused.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.
[ Example ]
Example 1. Preparation and method of experiment
1-1. Preparation of pepper fermented extract
Bacillus amyloliquefaciens strain 26N (accession number KCCM 11287P) was used for pepper fermentation. The strains were obtained from meju prepared conventionally using the medium of tryptic case soybean agar (Difco Co., USA) And cultured separately.
Specifically, black pepper and water were mixed in a weight ratio of 1:10, and Bacillus amyloliquefacience was inoculated to a total of 1%, and then the mixture was incubated at 37 ° C and 170 rpm at 0, 8, or 12 days (shaking incubation) to ferment the pepper. Then, the culture solution was recovered, filtered with filter paper, concentrated and lyophilized to prepare a black pepper fermented extract.
1-2. Mice immunized with OVA Splenocyte culture
To induce allergy to female BALB / c mice at 5 weeks of age, 10 μg of ovalbumin (OVA) and 1 mg of alum were mixed for 30 minutes, and 100 μl / (N = 5) twice a week. One week after the immunization, the mice were sacrificed by cervical disassembly, and the spleen was removed from the aseptic state. Then, the spleen was transferred to a small petri dish containing 1 ml of the basic medium, i.e., 2-mercaptoethanol and RPMI1640 medium supplemented with antibiotics And stored on ice. The spleen was then single-celled using a mesh, washed twice with 5 ml of basal medium, and then centrifuged at 1500 rpm for 5 minutes. After removing the supernatant, 1 ml of RBC (red blood cell) lysing buffer (SIGMA R7757) was added and incubated for 3 minutes on ice. 10 ml of basal medium was further added, ≪ / RTI > centrifugation twice.
On the other hand, pepper fermented extracts (0day, 8day, and 12day) prepared according to the method of Example 1-1 were treated with OVA as an antigen at a concentration of 100 μg / Or 50 / / ml, and the mouse spleen cells cultured by the above method were treated at 5 × 10 6 cells / well. After incubation in a CO 2 incubator at 37 ° C for 72 hours, the cell culture medium was recovered.
1-3. Using ELISA Splenocyte Cytokine analysis of culture medium
In order to measure the amount of IL-4 cytokine from the culture medium of the mouse spleen cells recovered according to the method of Example 1-2, an IL-4 BD OptEIATM MOUSE ELISA kit and a TGF-β kit (R & D Systems).
Specifically, a capture antibody was added to a 96-well plate and reacted overnight at 4 ° C. The plate was coated with an antibody, washed with a washing buffer (phosphate buffered saline with
Example 2. Th1 / Th2 Cytokine-modulating activity test
The immune balance regulated by cytokines produced by Th1 and Th2 cells is called Thl / Th2 balance. Thl cells are important for the regulation of cellular immunity, and Th2 cells play an important role in the regulation of humoral immune. Th1 / Th2 balance is maintained constantly by interfering with cytokine mainly on IFN-γ and cytokine mainly on IL-4, which are important for differentiation of Th2, If broken, various immune diseases can be induced. For example, in the case of deflecting Thl, cellular immunity is resurfaced to enhance infection resistance, and when deflected to Th2, infection resistance is reduced, and conversely, allergy is enhanced.
Therefore, the amount of IL-4 cytokine was measured from the culture medium of mouse spleen cells by the methods of Examples 1-2 and 1-3 in order to evaluate the inhibitory activity of Th2 immune response by the pepper fermented extract.
Comparing the inhibitory activity of IL-4 production by each extract, the inhibitory activity of IL-4 was shown at 50 ㎍ / ㎖ of non-fermented pepper extract (0 day), while the extract of fermented black pepper (8 days), the extracts (12 days) fermented for 12 days at the concentration of 25 ㎍ / ㎖ showed the same IL-4 inhibitory activity as the unfermented pepper extract at the concentration of 12.5 ㎍ / ㎖ Respectively. In other words, it was found that the activity of inhibiting IL-4 was enhanced about 4 times by the fermentation process of pepper.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. There will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.
Claims (7)
Characterized in that the fermentation extract is obtained by inoculating Bacillus amyloliquefaciens into a black pepper extract and culturing.
Wherein the fermentation extract is obtained by inoculating Bacillus amyloliquefaciens into a black pepper extract and culturing for 5 to 15 days.
Characterized in that the extraction solvent is selected from the group consisting of water, C 1 to C 4 lower alcohols, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, .
Wherein said composition inhibits the production of IL-4.
Characterized in that the fermentation extract is obtained by inoculating Bacillus amyloliquefaciens into a black pepper extract and culturing.
Characterized in that the fermentation extract is obtained by inoculating Bacillus amyloliquefaciens into a black pepper extract and culturing.
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