KR101808028B1 - Manufacturing method of powder of Tenebrio molitor larva using wet-grinding and spray drying method - Google Patents

Abstract

The present invention relates to a method for manufacturing a powder of Tenebrio molitor larva using wet-grinding and spray drying methods, wherein the powder of Tenebrio molitor larva manufactured by the method of the present invention, compared with a conventional brown larvae powder manufactured by a direct larval drying method and a mechanical pulverization method, has excellent powdery fluidity and particle size which are good to be manufactured for various foods or pharmaceutical compositions, and does not get rancid while containing a large amount of unsaturated fatty acid which is effective for enhancing the physiological activity.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a powder of brown larvae using a wet grinding method and a spray drying method,

The present invention relates to a method for producing powder of brown larch larva using a wet grinding method and a spray drying method.

The brown duck ( Tenebrio molitor ) is an insect of the beetle, and is also known as brown rice dung. It is distributed in Korea and other parts of the world. It is an insect that undergoes a complete transformation of eggs, larvae, pupae, and adults and is currently producing large-scale domestic breeding farms. The larval period is about 10 weeks, but about 2 weeks when artificial breeding.

Unlike other insects, brown ducks are relatively domesticated for a short period of transformation, and are largely kept for pets. Brown larvae are used as food worms for mealworms because they have high protein content, high growth rate and very high reproductive rate Because it is known.

It is also adaptable and easy to learn breeding techniques, so it is suitable for intensive processing because it can be raised in a factory or dispersed breeding, and it breeds in winter, so it can breed four seasons regardless of season.

The brown duck is easy to breed and can be easily mass-grown and has high nutritional value. Therefore, there is a growing interest in development of industrial applications such as feed, edible and the like, and related prior arts include the manufacture of brown goat larva powder by freeze- Korean Patent No. 10-1510260 which discloses a method is known. In addition, there has been little research on edible use of such brown goats. In addition, insect food has been replaced by new preservation methods due to the material characteristics of insect specimens, while eliminating external aversion and odor. Processing method is necessary.

The inventors of the present invention completed the present invention upon studying various physiological activities of brown gill, confirming that the method of wet-milling and spray-drying larva of brown gill is useful for stable and long-term storage of an oil-soluble substance of brown gill.

Korean Registered Patent No. 10-1510260 (Title of the invention: Method for producing edible brown gill larva powder and edible brown gill larva powder prepared by the same, applicant: Korea, registered on April 02, 2015) Korean Patent Laid-Open No. 10-2009-0111440 (Name of the invention: Feed Additive Containing Brown Dough and Functional Feed Using It, Applicant: Industry-Academic Cooperation of Gyeongsang National University, Publication Date: May 25, 2011)

It is an object of the present invention to provide a method for producing powder of brown larvae using a wet grinding method and a spray drying method.

The present invention relates to a method for cultivating a brown larva,

(Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae;

(Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate;

(Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And

(Step 5) spray drying the emulsion obtained in the fourth step;

And a method of spray drying the same.

Increase of the mealworm larvae of the first stage, to fast, to a mealworm larvae 1-3 days the slurry was added 1-3 times the weight of purified water to the discharge, the pressure 1.1 ~ 1.5kgf / cm ² and It can be carried out at a high temperature of 110 to 150 ° C for 30 to 120 minutes.

The homogenization in the second step is carried out by homogenizing at 800 to 20,000 rpm for 5 to 15 minutes.

The concentration of the wet pulverized product in the third step can be performed so that the sugar content of the final concentrate is 20 to 30 Bricks.

As the emulsifier in the fourth step, one or more selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, sugar ester, sorbitan fatty acid ester, lecithin, enzyme digesting lecithin, alginic acid, have.

In the fourth step, the oxidation stabilizer may include at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbic acid, lecithin, citric acid, and beta carotene.

The emulsification of the concentrate in the fourth step may be performed by sonication for 5 to 15 minutes. Also, it may be preliminarily emulsified by pulverizing using a homogenizer for 1 to 10 minutes before the ultrasonic treatment.

Therefore, the present invention can provide a powder of the larvae of the larvae prepared by the above method.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing powder of brown larch larva using a wet grinding method and a spray drying method. Preferably (step 1), there is a step of increasing brown larvae; (Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae; (Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate; (Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And (5) spray drying the emulsion obtained in the step (4). The present invention also provides a method for producing a larval larva of a larva using the wet pulverization method and the spray drying method.

In the preparation of the brown goat larva powder of the present invention, the first step is a step of growing a brown goat larva. In order to increase the brown goat larva of the first step, the brown larva is firstly fasted for 1 to 3 days 1 to 3 times the weight of purified water was added to the discharged fecal matter to obtain a pressure of 1.1 to 1.5 kgf / cm ² and At a temperature of 110 DEG C to 150 DEG C for 30 to 120 minutes. When the temperature is higher, the denaturation of the protein is a concern. On the other hand, if the temperature is too low, softening of the shell may be difficult. In addition, if the increase time is long, the denaturation of the nutrients may be worse. On the contrary, if the time is short, the softening of the skin may be difficult, and the preferable time is 60 minutes.

In the production of the brown goat larva powder of the present invention, the second step is a step of homogenizing the wet brown larvae and wet grinding the same. At this time, purified water having a weight of 1 to 3 times the weight of the boiled water is added to the boiled water in the first step and is homogenized by pulverizing with a homogenizer. The homogenization is carried out by using a homogenizer at 800 to 20,000 rpm, preferably 10,000 rpm for 5 to 15 minutes, preferably 10 minutes.

The homogenized liquid phase may be pulverized to a mesh size of 50 mesh or more at a bead size of 0.4 mm and a rotor speed of 3,000 rpm using a wet pulverizer by adding 1 to 3 times of purified water to the liquid phase weight.

The pulverization methods commonly used in the art are dry pulverization and wet pulverization. In the dry pulverization, when the fat content among the components contained in the pulverized product is large, for example, the non-defatted brown larva used in the present invention When the sample is crushed, the crushing is not performed well or the particle size of the crushed powder is not uniform, which is not suitable. Since the particle size of the powder may affect the texture, physical properties and storage stability of the product, it is very important to prepare a powder having homogeneous physical properties. On the other hand, wet milling can be milled even if the milled product contains a large amount of fat. Therefore, in the present invention, a wet grinding method is used to mill larvae of brown duck.

The first step and the second step can soften the epidermis of the larvae of brownie gruel to facilitate the production of the powder in the final step. If the epidermis is not well softened, most of the epidermis that does not have a good powdering ability can be discarded. Therefore, the method for preparing the powdery brown larvae containing the potato method is economical because it can increase the final powder yield. Chitin is also found in invertebrates such as spiders and insects and in some prototypes and is insoluble in water, dilute acid, alkali and most organic solvents. The contents of chitin in the larvae of brown goat larvae have not been elucidated yet. However, in the larvae of silkworm larvae, 44.2% of chitin, 55.8% of proteins, and 23.7% of chitin and 76.3% Sehwa Publishing Co.), it can be deduced that a large amount of chitin is contained in the brown goose larva. Therefore, it can be said that the supply of the kitchen quality contained in the brown goat larva powder was facilitated through the softening of the brown gruel epidermis.

In the preparation of the powder of the brown larvae of the present invention, the third step is the concentration step. In the present invention, the wet pulverized product of the second stage may be concentrated at a temperature of 50 to 65 ° C and a vacuum of 300 to 400 mmHg at a gauge pressure of 10 to 35 brix, preferably 20 It is appropriate to be ~ 30 bricks.

In the fourth step, the emulsifying agent and the oxidizing stabilizer are added to the concentrate obtained in the third step and emulsified to obtain an emulsion. Brown goat larvae contain about 35% of crude fat, and if they are stored for long periods without any additional oxidative stabilizing treatment, rancidity can be a concern. It has also been proposed to degrease brown goat larvae by processing them and pulverize them. However, since lipid-soluble substances are contained in the useful substances contained in the brown goat larva having physiological activity, the crude fat content in brown goat larva powder It is necessary to make fine powdering technology to increase the storage stability while enhancing the storage stability. In the present invention, an emulsifier and an oxidizing stabilizer may be added in order to improve the preservation and emulsification stability of the larval larvae and the emulsifying ability of the prepared powder after dissolution. The emulsifier may be at least one selected from the group consisting of glycerin fatty acid esters, polyglycerin fatty acid esters, sugar esters, sorbitan fatty acid esters, lecithin, enzyme digesting lecithin, alginic acid, carrageenin and guar gum A glycerin fatty acid ester may be added. The emulsifier may be added to the concentrate obtained in the third step in an amount of 0.2% by weight. For the oxidation stability of the powder, it is possible to use at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbate, lecithin, citric acid and beta carotene as oxidation stabilizers, Can be added. The oxidation stabilizer may be added to the concentrate obtained in the third step in an amount of 0.2% by weight.

The emulsification of the concentrate in the fourth step may be performed by sonication for 5 to 15 minutes. Also, it may be preliminarily emulsified by pulverizing using a homogenizer for 1 to 10 minutes before the ultrasonic treatment. Ultrasonic processing can be performed using an ultrasonic gauge (Sonics, USA), and the preliminary emulsification can be performed by using a homogenizer (homogenizer) at 500 to 2000 rpm, preferably 1000 rpm.

In the preparation of the powder of the brown larvae of the present invention, the fifth step relates to spray drying. In the present invention, the emulsion obtained in the fourth step is pulverized using a spray drier. As the conditions of spray drying at this time, it is preferable that the inlet temperature of the spray dryer is 150 to 200 ° C and the outlet temperature is 50 to 100 ° C, preferably the inlet temperature is 170 to 190 ° C and the outlet temperature is 80 to 90 ° C much better. Also, it is preferable that the spraying pressure of the spray dryer is 1.5 kgf / cm 2 or more and the rotation speed of the disk is 7,000 to 10,000 rpm. In the present invention, by using the spray drying method as described above, it is possible to perform the manufacture of the brown gruel powder more quickly and easily, compared with the freeze-drying method in which the time and cost are limited, Can be easily performed.

The present invention also provides a pharmaceutical composition for the treatment of various diseases, including the powder of the larvae of Brown Rhinoceros. The pharmaceutical compositions may be formulated in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions and the like according to conventional methods. Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have.

The dosage of the pharmaceutical composition of the present invention will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. The powder of the brown goat larva of the present invention is a drug which can be safely used for prolonged use for prevention because there is little toxicity and side effects.

In addition, the present invention provides a health functional food for the treatment of various diseases including powdery brown larvae and food acceptable food-aid additives. The brown gourd larva powder may be added in an amount of 0.001 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various kinds of drinks, meat, sausage, bread, Snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamin complexes.

The present invention relates to a method for preparing a brown goat larva powder by a wet pulverization method and a spray drying method. The brown goat larva powder prepared by the method of the present invention can be prepared by a direct larval drying method and a mechanical pulverization method Of the present invention has an advantage in that it has powdery fluidity and particle size which are good for preparing with various food or pharmaceutical compositions as compared with the brown goat larva powder of the present invention and contains a large amount of unsaturated fatty acid which is effective for enhancing the physiological activity, . Thus, the brown goat larva powder can be easily used as a pharmaceutical composition for treating various diseases or as a food for health promotion.

FIG. 1 is a photograph of a larvae of brown mallow larvae prepared by the method of Example 2 using the larvae obtained from Example 1 of the present invention.
Fig. 2 shows photographs of larvae of brown larvae prepared by ball milling under the condition of Comparative Example 5. Fig.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Preparation of pulverized product of brown larvae using wet pulverization method and spray drying method >

A wet pulverized material was first prepared to prepare brown goat larvae powder. For this purpose, a 8-year-old brown duck ( Tenebrio molitor ) larvae were purchased from a professional farming house and fasted for 2 days. The larvae were washed twice or three times with flowing water, and then 500 g Then, the same weight of purified water was added to the solution. The conditions of the casting were "normal pressure (1.0 kgf / cm ² ) and 95 ° C", "1.3 kgf / cm ² And 121 < 0 > C ", respectively. After the addition, the mixture was homogenized by a homogenizer (T25 Basic, IKA, Germany) at 10,000 rpm for 10 minutes and homogenized. To the homogenized liquid phase, purified water of twice the volume of the liquid phase was added and the mixture was dispersed in a wet grinder (LSI, NEYZSCH, Srlb Bavaria, Germany) was pulverized with a bead size of 0.4 mm and a rotor speed of 3,000 rpm to prepare a crushed larvae of brown goat larvae.

≪ Example 2: Emulsification and homogenization of pulverized product >

The larvae of the larvae obtained in Example 1 (1.3 kgf / cm ² and those grown at 121 ° C) were concentrated in vacuo at a temperature of 50 to 65 ° C and a vacuum of 300 to 400 mmHg gauge pressure at 25 brix A concentrate was obtained. To this concentrate, glycerin fatty acid ester and mixed tocopherol were added so as to be 0.2 wt%, respectively, followed by pulverization with a homogenizer (T25 Basic, IKA, Germany) at 1,000 rpm for 5 minutes to preliminarily emulsify. Then, the mixture was ultrasonicated by ultrasound sonicator (Sonics, USA) for 10 minutes and emulsified, followed by spray drying to obtain powder (brownish larval larvae) and stored at room temperature.

A photograph of the thus obtained brown dung larvae powder is shown in Fig.

≪ Comparative Example 1 > Powder prepared without addition of glycerin fatty acid ester and mixed tocopherol >

Powder was prepared in the same manner as in Example 2 using the brown larch larvae obtained in Example 1 except that glycerin fatty acid ester and mixed tocopherol were not added to the vacuum concentrated concentrate and emulsified immediately with a homogenizer and an ultrasonicizer Followed by spray drying to prepare brown goose larvae powder.

<Comparative Example 2> Powder prepared without adding glycerin fatty acid ester>

Powder was prepared in the same manner as in Example 2 except that the glycerin fatty acid ester was not added to the vacuum concentrated concentrate, and the emulsion was directly spray-dried using a homogenizer and an ultrasonicizer, Brown goat larvae powder was prepared.

&Lt; Comparative Example 3 > Powder prepared without adding mixed tocopherol &gt;

The powder was prepared in the same manner as in Example 2 except that the mixture of tocopherols was not added to the concentrated concentrate and the mixture was emulsified with a homogenizer and an ultrasonicizer. Larvae larvae were prepared.

&Lt; Comparative Example 4 > Powder prepared from defatted brown goat larvae &gt;

A 8-year-old brown duck ( Tenebrio molitor ) larva was purchased from a professional breeding farm and fasted for 2 days. The brown duck larvae which had been completely discharged were washed 2-3 times with water and dried in a microwave oven for 15 minutes . The dried larvae were then degreased at a pressure of 600 kgf / cm &lt; ² &gt; for 20 minutes using a Myungjin machine (sesame oil machine). Thereafter, the defatted larvae were dry-pulverized in a state of being mounted on a 50-mesh sieve using a pin-crusher (KARI INDUSTRIAL CO., LTD.) To obtain larval larvae. The moisture content of the thus obtained larvae was 5%.

&Lt; Comparative Example 5 &lt; tb &gt; Powdery brown larvae obtained by roll milling &

A 8-year-old brown duck ( Tenebrio molitor ) larva was purchased from a professional breeding farm and fasted for 2 days. The brown duck larvae which had been completely discharged were washed 2-3 times with water and dried in a microwave oven for 15 minutes . Thereafter, the dried larva was pulverized at a roll rotation speed of 150 rpm.

A photograph of the brown gill larva powder thus obtained is shown in Fig. Referring to the photograph of FIG. 2, it is confirmed that the particles of the powder obtained by roll milling in FIG. 2 are very rough and large as compared with the spray dried powder of FIG.

&Lt; Comparative Example 6 >

Tenebrio molitor larvae were purchased from professional breeding farms and fasted for 2 days. The larvae were washed 2 ~ 3 times with running water. The brown larvae were kept at 115 ℃ and 1.3 kgf / cm ² was sterilized by high temperature and high pressure for 5 minutes and then poured liquid nitrogen for freeze-drying. After rapid freezing, it was frozen in an ultra-low temperature freezer at 70 ° C for 24 hours and then sterilized at 115 ° C for 5 minutes. Followed by drying to remove moisture. The brown goat larvae having a particle size of about 1.4 mm were prepared by dividing the larvae of the brown goat with moisture removed by a mixer.

&Lt; Comparative Example 7: Powder prepared by adding dextrin without performing the concentration step >

Except that the mixture of the glycerin fatty acid ester and mixed tocopherol was added to the larvae of the larvae in the same amount as in Example 2 except for the step of preparing powder by the method of Example 2 using the brown larch larvae obtained in Example 1, And the same amount of dextrin was added to the mixed type tocopherol. The powder was then prepared by performing the following steps.

At this time, dextrin was used as an excipient added to the solution for spray drying. In general, for spray drying, dextrin must be added to the liquid phase of the spraying. As a preliminary experiment, it was tried to spray-dry the dextrin without adding dextrin after adding glycerin fatty acid ester and mixed tocopherol to the larvae of the larvae. It was not stable and spray drying was almost impossible.

<Experimental Example 1> Determination of particle size characteristics of brown larch larvae according to growing conditions>

According to the present invention, the crushed larvae of the larvae obtained in Example 1, which is an intermediate product for producing brown larch larvae, were sieved through a 50-mesh sieve, and they were passed through a sieve and collected in a hot-air drier at 80 ° C for 6 hours Dried, and the content of the dried powder at each condition is shown in Table 1 below. At this time, as a comparative condition, a wet pulverized product except for the increase step was also prepared.

As shown in Table 1, the amount (dried matter) passed through the 50 mesh of the wet pulverized product by the method of increasing the larvae in the larvae grown from the larvae not in the non-expanded larvae was increased by adding the temperature, Was more than that in the case of high pressure. By increasing the amount of time, the amount of dry matter was increased as the time of capital increase was longer, but the economic increase time was 60 minutes in both normal pressure and high temperature conditions.

Type of pulverized water Increase time (minutes) The dried structure passed through 50 mesh Weight (g) ratio(%) The pulverized product A (wet pulverization without the addition) 0 57.75 55
Pulverized material B (wet pulverization at atmospheric pressure, at 95 캜)
30 62.25 65 60 73.50 70 120 75.60 72 Crushed product C
(1.3kgf / cm ² , wet pulverization at 121 ° C) 30 84.00 80 60 89.25 85 120 90.30 86

< Experimental Example  2. Peroxide value and Lipid rancidity  Research>

Oxidative stability of the brown larch larvae obtained in Example 2 and Comparative Examples was examined as follows.

Experimental Example 2-1. Peroxide value

Table 2 shows the results of measuring the peroxide value at 0, 7, 14, and 21 days after storage at room temperature while storing 50 g of the larvae obtained from Example 2 and each comparative example. The peroxide value was measured according to the food revolution method. To this end, the oil was extracted from 50 g of each powder stored at room temperature under the conditions shown in Table 2, 25 mL of acetic acid: chloroform (3: 2, v / v) was added to each 5 g, and KI saturated solution 1 mL was added and stirred. After stirring, the reaction mixture was allowed to stand for 10 minutes, and then 75 mL of water was added. After the end of the disappearance of the blue color of the 0.01 N Na ₂ S ₂ O ₃ solution with the 1% (v / v) And repeatedly measured. At this time, the retentive extraction was obtained by shaking and allowing the filtrate to be concentrated in vacuo in 50 g of powder, using 200 mL of n-hexane as a solvent for 5 hours at room temperature.

The peroxide value of the powder of Example 2 was kept low even after 14 days of storage, but that of the powder of Comparative Example was rapidly increased after 14 days.

 Powder type storage duration 0 days 7 days 14 days 21st The powder of Comparative Example 1 5.13 meq / kg 5.57 meq / kg 7.02 meq / kg 8.35 meq / kg The powder of Comparative Example 2 5.13 meq / kg 5.68 meq / kg 6.99 meq / kg 8.23 meq / kg The powder of Comparative Example 3 5.12 meq / kg 5.75 meq / kg 7.21 meq / kg 8.45 meq / kg The powder of Comparative Example 5 5.14 meq / kg 5.95 meq / kg 7.04 meq / kg 8.96 meq / kg The powder of Comparative Example 6 5.12 meq / kg 5.74 meq / kg 7.23 meq / kg 8.80 meq / kg The powder of Example 2 5.12 meq / kg 5.18 meq / kg 5.82 meq / kg 6.31 meq / kg

Experimental Example  2-2. Lipid rancidity

Table 3 shows the results of measurement of lipid peroxidation (TBA) at 0, 7, 14, and 21 days after storage at room temperature while the brown larch larvae obtained in Example 2 and Comparative Examples were stored at room temperature. To this, 12.5 mL of 20% trichloroacetic acid solution was added to 5 g of brown larch larvae, and the mixture was homogenized at 14,000 rpm for 1 minute, diluted to 50 mL with distilled water, and then filtered through filter paper (Whatman No. 1) 5 mL was mixed with 5 mL of 2-thiobarbituric acid solution and left at room temperature for 20 hours. The absorbance was measured at 530 nm using a spectrophotometer.

In case of the powder of Example 2, the fat acid ratios were constantly maintained low after 14 days of storage, but the powder of Comparative Examples was rapidly increased after 14 days.

 Powder type storage duration 0 days 7 days 14 days 21st The powder of Comparative Example 1 0.212 mg MDA / kg 0.220 mg MDA / kg 0.331 mg MDA / kg 0.458 mg MDA / kg The powder of Comparative Example 2 0.205 mg MDA / kg 0.232 mg MDA / kg 0.375 mg MDA / kg 0.434 mg MDA / kg The powder of Comparative Example 3 0.203 mg MDA / kg 0.220 mg MDA / kg 0.386 mg MDA / kg 0.467 mg MDA / kg The powder of Comparative Example 5 0.217 mg MDA / kg 0.232 mg MDA / kg 0.320 mg MDA / kg 0.457 mg MDA / kg The powder of Comparative Example 6 0.203 mg MDA / kg 0.245 mg MDA / kg 0.325 mg MDA / kg 0.485 mg MDA / kg The powder of Example 2 0.202 mg MDA / kg 0.216 mg MDA / kg 0.217 mg MDA / kg 0.225 mg MDA / kg

< Experimental Example  3. Brown ass  Investigation of Quality Characteristics of Larval Powder>

Table 4 shows the crude fat and crude protein content, particle size and powder fluidity of the brown larch larvae obtained in Example 2 and Comparative Examples. The analytical method was based on the process analysis method of the Association of Certified Analytical Chemists (AOAC). That is, crude fat content was analyzed by ether extraction method and crude protein content was analyzed by Kjedahl nitrogen determination method. Powder particle size was determined by dividing the powder into a 50 mesh sieve and an amount not passed through the sieve.

Powder type Crude fat content (%) Crude protein content (%) Powder particle size (%)
(50 mesh body throughput) Powder fluidity Comparative Example 4 18.36 + - 0.10 64.65 + 0.79 92 ○ Comparative Example 5 35.83 + - 0.12 58.39 + - 0.45 5 X Comparative Example 7 34.10 ± 0.15 55.46 + - 0.42 46 X Example 2 36.12 ± 0.02 59.32 + 0.03 100 ◎

* Fluidity of powder is good: ◎, medium: ○, very poor: marked with X

Referring to Table 4, the powder of Comparative Example 4, which was degreased and dry-pulverized, had good powder particle size and powder flowability, but had a crude fat content of 18.36%, which was smaller than those of Comparative Example 5 and Example 2 (35.83 and 36.12%). The powder of Comparative Example 5 obtained by roll milling showed a crude fat content of 35.83%, but the powder particle size and the fluidity of the powder were very low. The powder of Example 2 had a crude fat content of 36.12%, and the powder particle size and the fluidity of the powder were good. On the other hand, the powder of Comparative Example 7, which was spray-dried without performing the concentration process, showed little difference in the crude fat content and the crude protein content, and it was found that the powder particle size and powder fluidity were not very good even though dextrin was added as an excipient for spray drying have.

&Lt; Preparation Example 1 > Pharmaceutical formulation - tablet &

200 g of the larval larvae powder obtained in Example 2 was mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

<Formulation Example 2: Food Preparation>

Formulation Example 2-1. Manufacture of cooking seasonings

The brown gourd larva powder obtained in Example 2 was added to the cooking seasoning at 1% by weight to prepare a cooking sauce for health promotion.

Formulation Example 2-2. Manufacture of flour food products

The brown gill larva powder obtained in Example 2 was added to the wheat flour at 0.1 wt%, and bread, cake, cookies, crackers and noodles were prepared by using the mixture to prepare a health improving food.

Preparation Example 2-3. Manufacture of soups and gravies

Health enhancing soup and juice were prepared by adding the brown gill larva powder obtained in Example 2 to the soup and juice at 0.1 wt%.

Formulation Example 2-4. Manufacture of dairy products

A brown larch larva powder obtained in Example 2 was added to milk in an amount of 0.1 wt%, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 2-5. Vegetable juice manufacturing

The vegetable juice for health promotion was prepared by adding 0.5 g of the larvae of the larvae obtained in Example 2 to 1,000 ml of tomato juice or carrot juice.

Formulation Example 2-6. Manufacture of fruit juice

Health enhancing fruit juice was prepared by adding 0.1 g of the brown larvae obtained in Example 2 to 1,000 ml of apple juice or grape juice.

Claims (9)

(Step 1) Increasing the larvae of brownie;
(Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae;
(Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate;
(Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And
(Step 5) spray drying the emulsion obtained in the fourth step;
Wherein the method comprises the steps of: (a) preparing a powder of a larval larva of brown gorilla by using a wet grinding method and a spray drying method. The method according to claim 1,
Increase of the mealworm larvae of the first stage, to fast, to a mealworm larvae 1-3 days the slurry was added 1-3 times the weight of purified water to the discharge, the pressure 1.1 ~ 1.5kgf / cm ² and Wherein the mixture is carried out at a high temperature of 110 to 150 DEG C for 30 to 120 minutes. The method according to claim 1,
Wherein the homogenization in the second step is carried out by homogenizing at 800 to 20,000 rpm for 5 to 15 minutes. The method for producing powder of brown larvae using the wet pulverization method and the spray drying method. The method according to claim 1,
Wherein the concentration of the wet pulverized product in the third step is performed such that the sugar content of the final concentrate is 20 to 30 brix. The method according to claim 1,
As the emulsifier in the fourth step, at least one selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, sugar ester, sorbitan fatty acid ester, lecithin, enzyme digesting lecithin, alginic acid, Wherein the powder is produced by a wet grinding method and a spray drying method. The method according to claim 1,
In the fourth step, the oxidative stabilizer includes at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbic acid, lecithin, citric acid and beta-carotene. Method for preparing powder of brown larvae using the drying method. The method according to claim 1,
Wherein the emulsification of the concentrate is performed by sonication for 5 to 15 minutes in the fourth step, followed by performing the wet pulverization method and the spray drying method. 8. The method of claim 7,
Wherein the step of pulverizing and pre-emulsifying the mixture using a homogenizer for 1 to 10 minutes before the ultrasonic treatment comprises a wet grinding method and a spray drying method. 9. A larva of a larva of a largemouth gourd, produced by the method of any one of claims 1 to 8.

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