KR101808028B1 - Manufacturing method of powder of Tenebrio molitor larva using wet-grinding and spray drying method - Google Patents
Manufacturing method of powder of Tenebrio molitor larva using wet-grinding and spray drying method Download PDFInfo
- Publication number
- KR101808028B1 KR101808028B1 KR1020160101909A KR20160101909A KR101808028B1 KR 101808028 B1 KR101808028 B1 KR 101808028B1 KR 1020160101909 A KR1020160101909 A KR 1020160101909A KR 20160101909 A KR20160101909 A KR 20160101909A KR 101808028 B1 KR101808028 B1 KR 101808028B1
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- South Korea
- Prior art keywords
- powder
- larvae
- brown
- wet
- spray drying
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/10—General methods of cooking foods, e.g. by roasting or frying
- A23L5/13—General methods of cooking foods, e.g. by roasting or frying using water or steam
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/40—Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/02—Antioxidant
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/20—Ingredients acting on or related to the structure
- A23V2200/222—Emulsifier
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- A23V2300/00—Processes
- A23V2300/26—Homogenisation
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- A23V2300/00—Processes
- A23V2300/46—Ultra high pressure
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
Description
본 발명은 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법에 관한 것이다. The present invention relates to a method for producing powder of brown larch larva using a wet grinding method and a spray drying method.
갈색거저리(Tenebrio molitor)는 딱정벌레목 거저리과의 곤충으로, 갈색쌀거저리 라고도 불리며 한국을 비롯하여 전 세계에 분포한다. 알, 유충, 번데기, 성충의 시기를 거치는 완전변태를 하는 곤충이며 현재 국내에 대량생산하고 있는 사육농가가 형성되어져 있다. 유충기간은 약 10주이나 인공 사육할 때에는 약 2주 정도이다. The brown duck ( Tenebrio molitor ) is an insect of the beetle, and is also known as brown rice dung. It is distributed in Korea and other parts of the world. It is an insect that undergoes a complete transformation of eggs, larvae, pupae, and adults and is currently producing large-scale domestic breeding farms. The larval period is about 10 weeks, but about 2 weeks when artificial breeding.
갈색거저리는 다른 곤충들과 달리 변태기간이 비교적 짧기 때문에 애완용으로 많이 사육되며, 갈색거저리의 유충은 밀웜(mealworm)이라 하여 먹이곤충으로 이용되는데 이는 동물 중에서 단백질 함량이 높고 성장이 빠르며 번식률이 매우 높은 것으로 알려져 있기 때문이다. Unlike other insects, brown ducks are relatively domesticated for a short period of transformation, and are largely kept for pets. Brown larvae are used as food worms for mealworms because they have high protein content, high growth rate and very high reproductive rate Because it is known.
또한 적응력이 강하고 사육기술을 익히기가 쉬워 공장에서 사육하거나 분산사육이 가능해 집중 가공에 적합하며, 겨울철에도 번식을 하므로 계절에 관계없이 4계절 사육할 수 있다. It is also adaptable and easy to learn breeding techniques, so it is suitable for intensive processing because it can be raised in a factory or dispersed breeding, and it breeds in winter, so it can breed four seasons regardless of season.
갈색거저리는 사육이 용이하여 손쉽게 대량증식이 가능하고 영양적인 가치가 높아 사료용, 식용 등 산업적 용도개발에 대한 관심은 증가되고 있으며, 관련 선행기술로는 동결건조법을 통해 갈색거저리 유충의 분말을 제조하는 방법이 개시된 한국등록특허 제10-1510260호 등이 공지되어 있다. 그러나, 이 외에 이러한 갈색거저리에 대한 식용으로써의 연구는 거의 진행된 바 없으며, 또한, 곤충의 식품화는 외형적 혐오감이나 특유의 악취 등을 없애면서도 곤충 시료가 갖는 재료적 특징으로 인한 새로운 보존법에 따른 가공방법이 필요한 실정이다. The brown duck is easy to breed and can be easily mass-grown and has high nutritional value. Therefore, there is a growing interest in development of industrial applications such as feed, edible and the like, and related prior arts include the manufacture of brown goat larva powder by freeze- Korean Patent No. 10-1510260 which discloses a method is known. In addition, there has been little research on edible use of such brown goats. In addition, insect food has been replaced by new preservation methods due to the material characteristics of insect specimens, while eliminating external aversion and odor. Processing method is necessary.
본 발명자들은 갈색거저리가 갖는 다양한 생리활성을 연구하던 중, 갈색거저리의 유충을 습식분쇄하여 분무건조하는 방법이 갈색거저리가 갖는 유용성 물질의 안정적이면서도 장기적인 보관에 유용함을 확인하여 본 발명을 완성하였다. The inventors of the present invention completed the present invention upon studying various physiological activities of brown gill, confirming that the method of wet-milling and spray-drying larva of brown gill is useful for stable and long-term storage of an oil-soluble substance of brown gill.
본 발명의 목적은 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법을 제공하는 데에 있다. It is an object of the present invention to provide a method for producing powder of brown larvae using a wet grinding method and a spray drying method.
본 발명은, (제1단계) 갈색거저리 유충을 증자하는 단계;The present invention relates to a method for cultivating a brown larva,
(제2단계) 상기 제1단계에서 증자된 갈색거저리 유충을 균질화하고 습식분쇄하여 갈색거저리 유충의 습식분쇄물을 얻는 단계; (Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae;
(제3단계) 상기 제2단계에서 얻은 습식분쇄물을 농축하여 농축물을 얻는 단계; (Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate;
(제4단계) 상기 제3단계에서 얻은 농축물에 유화제와 산화안정제를 첨가하고 유화하여 유화물을 얻는 단계; 및,(Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And
(제5단계) 상기 제4단계에서 얻은 유화물을 분무건조하는 단계; (Step 5) spray drying the emulsion obtained in the fourth step;
를 포함하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법을 제공한다. And a method of spray drying the same.
상기 제1단계의 갈색거저리 유충의 증자는, 갈색거저리 유충을 1~3일간 금식시켜 배설물이 배출된 것에 1~3배 중량의 정제수를 첨가하여, 압력 1.1~1.5kgf/cm2 및 온도 110℃~150℃의 가압고온 조건에서, 30~120분간 수행할 수 있다. Increase of the mealworm larvae of the first stage, to fast, to a mealworm larvae 1-3 days the slurry was added 1-3 times the weight of purified water to the discharge, the pressure 1.1 ~ 1.5kgf / cm 2 and It can be carried out at a high temperature of 110 to 150 ° C for 30 to 120 minutes.
제2단계의 균질화는 균질기로 800~20000rpm에서 5~15분간 분쇄하여 수행하는 것을 특징으로 한다. The homogenization in the second step is carried out by homogenizing at 800 to 20,000 rpm for 5 to 15 minutes.
상기 제3단계에서 습식분쇄물의 농축은, 최종 농축물의 당도가 20~30 브릭스가 되도록 수행할 수 있다. The concentration of the wet pulverized product in the third step can be performed so that the sugar content of the final concentrate is 20 to 30 Bricks.
상기 제4단계에서 유화제로는 글리세린지방산 에스테르, 폴리글리세린 지방산에스테르, 슈가에스텔, 솔비탄 지방산 에스테르, 레시틴, 효소분해 레시틴, 알긴산, 카라기닌 및 구암검으로 이루어진 군에서 선택되는 1종 이상이 포함될 수 있다. As the emulsifier in the fourth step, one or more selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, sugar ester, sorbitan fatty acid ester, lecithin, enzyme digesting lecithin, alginic acid, have.
상기 제4단계에서 산화안정제로는 혼합형토코페롤, 녹차추출물분말, 아스코빈산, 아스코빈산 나트륨, 레시틴, 구연산 및 베타카로틴으로 이루어진 군에서 선택되는 1종 이상이 포함될 수 있다. In the fourth step, the oxidation stabilizer may include at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbic acid, lecithin, citric acid, and beta carotene.
상기 제4단계에서 농축물의 유화는 5~15분간 초음파 처리하여 수행할 수 있다. 또한 상기 초음파 처리 전에 1~10분간 균질기를 이용하여 분쇄하여 예비 유화하는 단계가 포함될 수 있다. The emulsification of the concentrate in the fourth step may be performed by sonication for 5 to 15 minutes. Also, it may be preliminarily emulsified by pulverizing using a homogenizer for 1 to 10 minutes before the ultrasonic treatment.
따라서 본 발명은 상기 방법으로 제조한 갈색거저리 유충의 분말을 제공할 수 있다. Therefore, the present invention can provide a powder of the larvae of the larvae prepared by the above method.
이하, 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법에 관한 것이다. 바람직하게는 (제1단계) 갈색거저리 유충을 증자하는 단계; (제2단계) 상기 제1단계에서 증자된 갈색거저리 유충을 균질화하고 습식분쇄하여 갈색거저리 유충의 습식분쇄물을 얻는 단계; (제3단계) 상기 제2단계에서 얻은 습식분쇄물을 농축하여 농축물을 얻는 단계; (제4단계) 상기 제3단계에서 얻은 농축물에 유화제와 산화안정제를 첨가하고 유화하여 유화물을 얻는 단계; 및, (제5단계) 상기 제4단계에서 얻은 유화물을 분무건조하는 단계;를 포함하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법을 제공한다. The present invention relates to a method for producing powder of brown larch larva using a wet grinding method and a spray drying method. Preferably (step 1), there is a step of increasing brown larvae; (Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae; (Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate; (Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And (5) spray drying the emulsion obtained in the step (4). The present invention also provides a method for producing a larval larva of a larva using the wet pulverization method and the spray drying method.
본 발명의 갈색거저리 유충의 분말 제조에 있어서, 상기 제1단계는 갈색거저리 유충을 증자하는 단계로서, 상기 1단계의 갈색거저리 유충의 증자를 위해, 먼저, 갈색거저리 유충을 1~3일간 금식시켜 배설물이 배출된 것에 1~3배 중량의 정제수를 첨가하여 압력 1.1~1.5kgf/cm2 및 온도 110℃~150℃의 가압고온 조건에서 30~120분간 증자하는 것을 특징으로 한다. 상기 증자 시의 온도가 높으면 단백질의 변성이 우려되고 반면 너무 낮으면 껍질의 연화가 잘 안될 수 있어 바람직한 온도는 121℃이다. 또한 증자 시간이 길면 영양성분의 변성이 우려되고, 반대로 시간이 짧으면 껍질의 연화가 잘 안될 수 있어 바람직한 시간은 60분이다. In the preparation of the brown goat larva powder of the present invention, the first step is a step of growing a brown goat larva. In order to increase the brown goat larva of the first step, the brown larva is firstly fasted for 1 to 3 days 1 to 3 times the weight of purified water was added to the discharged fecal matter to obtain a pressure of 1.1 to 1.5 kgf / cm 2 and At a temperature of 110 DEG C to 150 DEG C for 30 to 120 minutes. When the temperature is higher, the denaturation of the protein is a concern. On the other hand, if the temperature is too low, softening of the shell may be difficult. In addition, if the increase time is long, the denaturation of the nutrients may be worse. On the contrary, if the time is short, the softening of the skin may be difficult, and the preferable time is 60 minutes.
본 발명의 갈색거저리 유충의 분말 제조에 있어서, 상기 제2단계는 증자된 갈색거저리 유충의 증숙물을 균질화하고 습식분쇄하는 단계이다. 이 때 제1단계에서 증자된 증숙물에, 증숙물 중량 1~3배의 정제수를 첨가하여 균질기(homogenizer)로 분쇄하여 균질화하는 것을 특징으로 한다. 상기 균질화는 균질기(homogenizer)를 이용하여, 800~20,000 rpm에서, 바람직하게는 10,000 rpm, 5~15분간, 바람직하게는 10분간 분쇄하여 수행하는 것을 특징으로 한다. In the production of the brown goat larva powder of the present invention, the second step is a step of homogenizing the wet brown larvae and wet grinding the same. At this time, purified water having a weight of 1 to 3 times the weight of the boiled water is added to the boiled water in the first step and is homogenized by pulverizing with a homogenizer. The homogenization is carried out by using a homogenizer at 800 to 20,000 rpm, preferably 10,000 rpm for 5 to 15 minutes, preferably 10 minutes.
상기의 균질화된 액상에, 상기 액상 중량의 1~3배의 정제수를 첨가하여 습식분쇄기를 이용하여 bead size 0.4 mm, rotor speed 3,000 rpm의 조건에서 50 메시(mesh)이상으로 분쇄할 수 있다. The homogenized liquid phase may be pulverized to a mesh size of 50 mesh or more at a bead size of 0.4 mm and a rotor speed of 3,000 rpm using a wet pulverizer by adding 1 to 3 times of purified water to the liquid phase weight.
당 업계에서 통상적으로 적용되는 분쇄방법은 건식분쇄 및 습식분쇄가 있는데, 건식분쇄는 피분쇄물이 함유하고 있는 성분 중 지방함량이 많을 경우, 예를 들어 본 발명에서 사용되는 탈지되지 않은 갈색거저리 유충 시료의 분쇄 시에는 분쇄가 잘되지 않거나 분쇄 이후의 분말의 입자크기가 균일하지 않아 적절하지 않다. 분말의 입자크기는 제품의 식감, 물성, 보존 안정성에 영향을 줄 수 있기 때문에 균질한 물성을 갖는 분말의 제조가 매우 중요하다. 이에 반해 습식분쇄는 피분쇄물에 지방이 많이 함유되어 있어도 분쇄가 가능하기에 본 발명에서는 갈색거저리의 유충의 분쇄를 위해 습식분쇄법을 이용한다. The pulverization methods commonly used in the art are dry pulverization and wet pulverization. In the dry pulverization, when the fat content among the components contained in the pulverized product is large, for example, the non-defatted brown larva used in the present invention When the sample is crushed, the crushing is not performed well or the particle size of the crushed powder is not uniform, which is not suitable. Since the particle size of the powder may affect the texture, physical properties and storage stability of the product, it is very important to prepare a powder having homogeneous physical properties. On the other hand, wet milling can be milled even if the milled product contains a large amount of fat. Therefore, in the present invention, a wet grinding method is used to mill larvae of brown duck.
상기 제1단계와 제2단계를 통해 갈색거저리 유충의 표피를 연화하여 최종단계에서의 분말 제조를 용이하게 할 수 있다. 표피의 연화가 잘되지 않을 경우, 분말화가 잘되지 않는 표피가 대부분 버려질 수 있다. 따라서 증자방법이 포함된 갈색거저리 유충의 분말 제조법은 최종적인 분말 수득율을 높일 수 있어 경제적이다. 또한 갈색거저리 유충의 표피(껍질)에는 키틴질이 포함되는데, 키틴질은 거미, 곤충류 등의 무척추동물과 일부 원생식물 중에 분포되어 있는 성분이며, 물, 묽은 산, 알칼리 및 대부분의 유기용매에 불용성이다. 갈색거저리 유충의 껍질에 함유되어 있는 키틴질의 함량은 아직까지 밝혀져 있지 않으나 누에의 유충 껍질에는 키틴 44.2%, 단백질 55.8%이며 메뚜기 날개에는 키틴 23.7%, 단백질이 76.3%로 구성되어 있는(화학대사전, 세화출판사) 것으로 보아 갈색거저리 유충의 껍질에도 상당량의 키틴질이 함유되었을 것으로 유추할 수 있다. 따라서 갈색거저리 표피의 연화를 통해 갈색거저리 유충의 분말에 포함된 키친질의 공급이 용이해졌다고 할 수 있다. The first step and the second step can soften the epidermis of the larvae of brownie gruel to facilitate the production of the powder in the final step. If the epidermis is not well softened, most of the epidermis that does not have a good powdering ability can be discarded. Therefore, the method for preparing the powdery brown larvae containing the potato method is economical because it can increase the final powder yield. Chitin is also found in invertebrates such as spiders and insects and in some prototypes and is insoluble in water, dilute acid, alkali and most organic solvents. The contents of chitin in the larvae of brown goat larvae have not been elucidated yet. However, in the larvae of silkworm larvae, 44.2% of chitin, 55.8% of proteins, and 23.7% of chitin and 76.3% Sehwa Publishing Co.), it can be deduced that a large amount of chitin is contained in the brown goose larva. Therefore, it can be said that the supply of the kitchen quality contained in the brown goat larva powder was facilitated through the softening of the brown gruel epidermis.
본 발명의 갈색거저리 유충의 분말 제조에 있어서, 상기 제3단계는 농축 단계이다. 본 발명에서는 상기 제2단계의 습식분쇄물을 50~65℃의 온도와 게이지압력 300~400 mmHg의 진공에서 농축할 수 있으며, 상기 농축물의 당도는 10~35 브릭스(brix), 바람직하게는 20~30 브릭스인 것이 적절하다. In the preparation of the powder of the brown larvae of the present invention, the third step is the concentration step. In the present invention, the wet pulverized product of the second stage may be concentrated at a temperature of 50 to 65 ° C and a vacuum of 300 to 400 mmHg at a gauge pressure of 10 to 35 brix, preferably 20 It is appropriate to be ~ 30 bricks.
본 발명의 갈색거저리 유충의 분말 제조에 있어서, 상기 제4단계는 제3단계에서 얻은 농축물에 유화제와 산화안정제를 첨가하고 유화하여 유화물을 얻는 단계이다. 갈색거저리 유충에는 조지방이 약 35% 정도 함유되어 있어, 별도의 산화 안정적인 처리 없이 갈색거저리 유충의 분말을 장기 저장할 경우 산패가 우려될 수 있다. 이에 갈색거저리 유충을 가공할 때 탈지하여 이를 분말화하는 것이 제안되기도 하였으나, 생리활성 작용이 있는 갈색거저리 유충에 포함된 유용성 물질들 중에 지용성 물질이 많이 포함되어 있기 때문에, 갈색거저리 유충 분말 내의 조지방 함유량을 높이면서도 보존 안정성을 높이기 위한 미세분말화 기술이 필요하다. 이에 본 발명에서는 갈색거저리 유충 분말의 보존과 유화 안정성 및 제조된 분말의 용해 후의 유화 능력을 향상시키기 위하여 유화제와 산화안정제를 첨가할 수 있다. 이때 유화제로는 글리세린지방산 에스테르, 폴리글리세린 지방산에스테르, 슈가에스텔, 솔비탄 지방산 에스테르, 레시틴, 효소분해 레시틴, 알긴산, 카라기닌 및 구암검으로 이루어진 군에서 선택되는 1종 이상을 사용할 수 있으나, 바람직하게는 글리세린지방산 에스테르가 첨가될 수 있다. 상기 유화제는 제3단계에서 얻은 농축물에 0.2 중량%가 되도록 첨가할 수 있다. 또한, 분말의 산화안정을 위해 산화안정제인 혼합형토코페롤, 녹차추출물분말, 아스코빈산, 아스코빈산 나트륨, 레시틴, 구연산 및 베타카로틴으로 이루어진 군에서 선택되는 1종 이상을 사용할 수 있으며, 바람직하게는 혼합형토코페롤이 첨가될 수 있다. 상기 산화안정제는 제3단계에서 얻은 농축물에 0.2 중량%가 되도록 첨가할 수 있다. In the fourth step, the emulsifying agent and the oxidizing stabilizer are added to the concentrate obtained in the third step and emulsified to obtain an emulsion. Brown goat larvae contain about 35% of crude fat, and if they are stored for long periods without any additional oxidative stabilizing treatment, rancidity can be a concern. It has also been proposed to degrease brown goat larvae by processing them and pulverize them. However, since lipid-soluble substances are contained in the useful substances contained in the brown goat larva having physiological activity, the crude fat content in brown goat larva powder It is necessary to make fine powdering technology to increase the storage stability while enhancing the storage stability. In the present invention, an emulsifier and an oxidizing stabilizer may be added in order to improve the preservation and emulsification stability of the larval larvae and the emulsifying ability of the prepared powder after dissolution. The emulsifier may be at least one selected from the group consisting of glycerin fatty acid esters, polyglycerin fatty acid esters, sugar esters, sorbitan fatty acid esters, lecithin, enzyme digesting lecithin, alginic acid, carrageenin and guar gum A glycerin fatty acid ester may be added. The emulsifier may be added to the concentrate obtained in the third step in an amount of 0.2% by weight. For the oxidation stability of the powder, it is possible to use at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbate, lecithin, citric acid and beta carotene as oxidation stabilizers, Can be added. The oxidation stabilizer may be added to the concentrate obtained in the third step in an amount of 0.2% by weight.
상기 제4단계에서 농축물의 유화는 5~15분간 초음파 처리하여 수행할 수 있다. 또한 상기 초음파 처리 전에 1~10분간 균질기를 이용하여 분쇄하여 예비 유화하는 단계가 포함될 수 있다. 이 때 초음파 처리는 울트라 소니게이터(Sonics, 미국)를 이용할 수 있고, 예비 유화는 균질기(호모게나이저)를 이용하여 500~2000 rpm, 바람직하게는 1000 rpm에서 분쇄하여 수행할 수 있다. The emulsification of the concentrate in the fourth step may be performed by sonication for 5 to 15 minutes. Also, it may be preliminarily emulsified by pulverizing using a homogenizer for 1 to 10 minutes before the ultrasonic treatment. Ultrasonic processing can be performed using an ultrasonic gauge (Sonics, USA), and the preliminary emulsification can be performed by using a homogenizer (homogenizer) at 500 to 2000 rpm, preferably 1000 rpm.
본 발명의 갈색거저리 유충의 분말 제조에 있어서, 제5단계는 분무건조에 관한 것이다. 본 발명에서는 분무건조기를 이용하여 제4단계에서 얻은 유화물을 분말화하였다. 이때의 분무 건조의 조건으로서, 분무건조기의 흡입구 온도가 150~200℃이고 배출구 온도가 50~100℃인 것이 좋으며, 바람직하게는 흡입구 온도는 170~190℃이고 배출구온도는 80~90℃인 것이 더 좋다. 또한 이 때 상기 분무건조기의 분무압력은 1.5kgf/㎠ 이상이고, 디스크의 회전속도는 7,000~10,000 rpm인 것이 바람직하다. 본 발명에서는 상기와 같은 분무건조방법을 이용함으로써, 시간 및 비용에 대한 제약이 따르는 동결건조방법을 이용하는 것에 비해 갈색거저리의 분말 제조를 보다 빠르고 용이하게 수행할 수 있고, 적은 비용으로 분말의 대량 제조를 용이하게 수행할 수 있다는 장점이 있다. In the preparation of the powder of the brown larvae of the present invention, the fifth step relates to spray drying. In the present invention, the emulsion obtained in the fourth step is pulverized using a spray drier. As the conditions of spray drying at this time, it is preferable that the inlet temperature of the spray dryer is 150 to 200 ° C and the outlet temperature is 50 to 100 ° C, preferably the inlet temperature is 170 to 190 ° C and the outlet temperature is 80 to 90 ° C much better. Also, it is preferable that the spraying pressure of the spray dryer is 1.5 kgf / cm 2 or more and the rotation speed of the disk is 7,000 to 10,000 rpm. In the present invention, by using the spray drying method as described above, it is possible to perform the manufacture of the brown gruel powder more quickly and easily, compared with the freeze-drying method in which the time and cost are limited, Can be easily performed.
또한, 본 발명은 상기 갈색거저리 유충의 분말을 포함하는 각종 질환의 치료용 약학 조성물을 제공한다. 상기 약학 조성물은 특히 각각 통상의 방법에 따라 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼 등의 경구형 제형의 형태로 제형화하여 사용될 수 있다. 상기 약학 조성물에 포함될 수 있는 담체, 부형제 및 희석제로는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로스, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유를 들 수 있다. 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 본 발명의 약학 조성물에 적어도 하나 이상의 부형제, 예를 들면, 전분, 탄산칼슘, 수크로스 또는 락토오스, 젤라틴 등을 섞어 조제된다. 또한 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용된다. 경구투여를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. The present invention also provides a pharmaceutical composition for the treatment of various diseases, including the powder of the larvae of Brown Rhinoceros. The pharmaceutical compositions may be formulated in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions and the like according to conventional methods. Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have.
본 발명의 약학 조성물의 투여량은 치료받을 대상의 연령, 성별, 체중과, 치료할 특정 질환 또는 병리 상태, 질환 또는 병리 상태의 심각도, 투여경로 및 처방자의 판단에 따라 달라질 것이다. 이러한 인자에 기초한 투여량 결정은 당업자의 수준 내에 있으며, 일반적으로 투여량은 0.01㎎/㎏/일~대략 2000㎎/㎏/일의 범위이다. 더 바람직한 투여량은 1㎎/㎏/일~500㎎/㎏/일이다. 투여는 하루에 한번 투여할 수도 있고, 수회 나누어 투여할 수도 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다. The dosage of the pharmaceutical composition of the present invention will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2000 mg / kg / day. A more preferable dosage is 1 mg / kg / day to 500 mg / kg / day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.
본 발명의 약학 조성물은 쥐, 가축, 인간 등의 포유동물에 다양한 경로로 투여될 수 있다. 본 발명의 갈색거저리 유충의 분말은 독성 및 부작용이 거의 없으므로 예방 목적으로 장기간 복용시에도 안심하고 사용할 수 있는 약제이다. The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. The powder of the brown goat larva of the present invention is a drug which can be safely used for prolonged use for prevention because there is little toxicity and side effects.
또한, 본 발명은 갈색거저리 유충의 분말 및 식품학적으로 허용 가능한 식품보조 첨가제를 포함하는 각종 질환의 치료용 건강기능식품을 제공한다. 상기 갈색거저리 유충의 분말은 본 발명의 건강기능식품에 0.001~100 중량%로 하여 첨가될 수 있다. 본 발명의 건강기능식품은 정제, 캡슐제, 환제 또는 액제 등의 형태를 포함하며, 본 발명의 추출물을 첨가할 수 있는 식품으로는, 예를 들어, 각종 드링크제, 육류, 소세지, 빵, 캔디류, 스넥류, 면류, 아이스크림, 유제품, 스프, 이온음료, 음료수, 알코올 음료, 껌, 차 및 비타민 복합제 등이 있다. In addition, the present invention provides a health functional food for the treatment of various diseases including powdery brown larvae and food acceptable food-aid additives. The brown gourd larva powder may be added in an amount of 0.001 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various kinds of drinks, meat, sausage, bread, Snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamin complexes.
본 발명은 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법에 관한 것으로서, 본 발명의 방법으로 제조한 갈색거저리 유충의 분말은, 직접적인 유충 건조방법과 기계적 분쇄법을 이용하여 제조한 종래의 갈색거저리 유충의 분말과 비교하여 각종 식품이나 약학 조성물로 제조하기에 좋은 분말 유동성과 입도를 가지고 있고, 생리활성 증진에 효과가 있는 불포화 지방산을 다량으로 포함하고 있으면서도 산패가 잘되지 않는다는 장점이 있다. 이에 상기 갈색거저리 유충 분말은 각종 질환의 치료용 약학 조성물 또는 건강증진용 식품으로 용이하게 이용될 수 있다. The present invention relates to a method for preparing a brown goat larva powder by a wet pulverization method and a spray drying method. The brown goat larva powder prepared by the method of the present invention can be prepared by a direct larval drying method and a mechanical pulverization method Of the present invention has an advantage in that it has powdery fluidity and particle size which are good for preparing with various food or pharmaceutical compositions as compared with the brown goat larva powder of the present invention and contains a large amount of unsaturated fatty acid which is effective for enhancing the physiological activity, . Thus, the brown goat larva powder can be easily used as a pharmaceutical composition for treating various diseases or as a food for health promotion.
도 1은 본 발명의 실시예 1에서 얻은 갈색거저리 유충 분쇄물을 이용하여 실시예 2의 방법으로 제조한 갈색거저리 유충 분말의 사진을 나타낸다.
도 2는 비교예 5의 조건으로 볼밀 분쇄하여 제조한 갈색거저리 유충 분말의 사진을 나타낸다. FIG. 1 is a photograph of a larvae of brown mallow larvae prepared by the method of Example 2 using the larvae obtained from Example 1 of the present invention.
Fig. 2 shows photographs of larvae of brown larvae prepared by ball milling under the condition of Comparative Example 5. Fig.
이하 본 발명의 바람직한 실시예를 상세히 설명하기로 한다. 그러나, 본 발명은 여기서 설명되는 실시예에 한정되지 않고 다른 형태로 구체화될 수도 있다. 오히려, 여기서 소개되는 내용이 철저하고 완전해지도록, 당업자에게 본 발명의 사상을 충분히 전달하기 위해 제공하는 것이다. Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.
<실시예 1. 습식분쇄법 및 분무건조법을 이용하여 갈색거저리 유충의 분쇄물 제조>≪ Example 1: Preparation of pulverized product of brown larvae using wet pulverization method and spray drying method >
갈색거저리 유충 분말을 제조하기 위해 먼저 습식분쇄물을 제조하였다. 이를 위해, 전문 사육농가에서 8령의 갈색거저리(Tenebrio molitor) 유충을 구입하여 2일간 금식시켜 배설물을 완전히 배출시킨 생것 상태의 갈색거저리 유충을 흐르는 물로 2~3회 수세한 후 증숙기에 500g을 넣은 다음 이에 동일 중량의 정제수를 넣고 증자하였다. 증자조건은 「상압(1.0kgf/cm2) 및 95℃」, 「1.3kgf/cm2 및 121℃」로 하고, 증자시간 또한 각각 달리하였다. 증자 후 균질기(homogenizer, T25 Basic, IKA, Germany)로 10,000 rpm에서 10분간 분쇄하여 균질화한 다음, 균질화된 액상에, 상기 액상의 2배 부피의 정제수를 첨가하여 습식분쇄기(LSI, NEYZSCH, Srlb Bavaria, Germany)를 이용하여 bead size 0.4 mm, rotor speed 3,000 rpm의 조건으로 이 액상을 분쇄하여 갈색거저리 유충의 분쇄물을 제조하였다. A wet pulverized material was first prepared to prepare brown goat larvae powder. For this purpose, a 8-year-old brown duck ( Tenebrio molitor ) larvae were purchased from a professional farming house and fasted for 2 days. The larvae were washed twice or three times with flowing water, and then 500 g Then, the same weight of purified water was added to the solution. The conditions of the casting were "normal pressure (1.0 kgf / cm 2 ) and 95 ° C", "1.3 kgf / cm 2 And 121 < 0 > C ", respectively. After the addition, the mixture was homogenized by a homogenizer (T25 Basic, IKA, Germany) at 10,000 rpm for 10 minutes and homogenized. To the homogenized liquid phase, purified water of twice the volume of the liquid phase was added and the mixture was dispersed in a wet grinder (LSI, NEYZSCH, Srlb Bavaria, Germany) was pulverized with a bead size of 0.4 mm and a rotor speed of 3,000 rpm to prepare a crushed larvae of brown goat larvae.
<실시예 2. 분쇄물의 유화 및 균질화>≪ Example 2: Emulsification and homogenization of pulverized product >
실시예 1에서 얻은 갈색거저리 유충 분쇄물(1.3kgf/cm2 및 121℃에서 증자한 것)을 50~65℃의 온도와 게이지압력 300~400 mmHg의 진공에서 25 브릭스(brix)로 진공농축하여 농축물을 얻었다. 이 농축물에 글리세린지방산 에스테르와 혼합형 토코페롤이 각각 0.2 중량%가 되도록 첨가한 후 균질기(homogenizer, T25 Basic, IKA, Germany)로 1,000 rpm에서 5분간 분쇄하여 예비유화하였다. 이 후 울트라 소니게이터(Sonics, 미국)로 10분간 초음파처리하여 유화시킨 후 분무건조하여 분말(갈색거저리 유충 분말)을 얻은 다음 실온에 저장하였다. The larvae of the larvae obtained in Example 1 (1.3 kgf / cm 2 and those grown at 121 ° C) were concentrated in vacuo at a temperature of 50 to 65 ° C and a vacuum of 300 to 400 mmHg gauge pressure at 25 brix A concentrate was obtained. To this concentrate, glycerin fatty acid ester and mixed tocopherol were added so as to be 0.2 wt%, respectively, followed by pulverization with a homogenizer (T25 Basic, IKA, Germany) at 1,000 rpm for 5 minutes to preliminarily emulsify. Then, the mixture was ultrasonicated by ultrasound sonicator (Sonics, USA) for 10 minutes and emulsified, followed by spray drying to obtain powder (brownish larval larvae) and stored at room temperature.
이렇게 얻은 갈색거저리 유충의 분말의 사진은 도 1에 나타내었다. A photograph of the thus obtained brown dung larvae powder is shown in Fig.
<비교예 1. 글리세린지방산 에스테르와 혼합형 토코페롤을 첨가하지 않고 제조한 분말>≪ Comparative Example 1 > Powder prepared without addition of glycerin fatty acid ester and mixed tocopherol >
실시예 1에서 얻은 갈색거저리 유충 분쇄물을 이용하여 실시예 2와 동일하게 분말을 제조하되, 진공농축된 농축물에 글리세린지방산 에스테르와 혼합형 토코페롤은 첨가하지 않고 바로 균질기와 울트라 소니게이터로 유화한 것을 분무건조하여 갈색거저리 유충 분말을 제조하였다. Powder was prepared in the same manner as in Example 2 using the brown larch larvae obtained in Example 1 except that glycerin fatty acid ester and mixed tocopherol were not added to the vacuum concentrated concentrate and emulsified immediately with a homogenizer and an ultrasonicizer Followed by spray drying to prepare brown goose larvae powder.
<비교예 2. 글리세린지방산 에스테르를 첨가하지 않고 제조한 분말><Comparative Example 2> Powder prepared without adding glycerin fatty acid ester>
실시예 1에서 얻은 갈색거저리 유충 분쇄물을 이용하여 실시예 2와 동일하게 분말을 제조하되, 진공농축된 농축물에 글리세린지방산 에스테르는 첨가하지 않고 바로 균질기와 울트라 소니게이터로 유화한 것을 분무건조하여 갈색거저리 유충 분말을 제조하였다. Powder was prepared in the same manner as in Example 2 except that the glycerin fatty acid ester was not added to the vacuum concentrated concentrate, and the emulsion was directly spray-dried using a homogenizer and an ultrasonicizer, Brown goat larvae powder was prepared.
<비교예 3. 혼합형 토코페롤을 첨가하지 않고 제조한 분말>≪ Comparative Example 3 > Powder prepared without adding mixed tocopherol >
실시예 1에서 얻은 갈색거저리 유충 분쇄물을 이용하여 실시예 2와 동일하게 분말을 제조하되, 진공농축된 농축물에 혼합형 토코페롤은 첨가하지 않고 바로 균질기와 울트라 소니게이터로 유화한 것을 분무건조하여 갈색거저리 유충 분말을 제조하였다. The powder was prepared in the same manner as in Example 2 except that the mixture of tocopherols was not added to the concentrated concentrate and the mixture was emulsified with a homogenizer and an ultrasonicizer. Larvae larvae were prepared.
<비교예 4. 탈지한 갈색거저리 유충으로 제조한 분말>≪ Comparative Example 4 > Powder prepared from defatted brown goat larvae >
전문 사육농가에서 8령의 갈색거저리(Tenebrio molitor) 유충을 구입하여 2일간 금식시켜 배설물을 완전히 배출시킨 갈색거저리 유충을 흐르는 물로 2~3회 수세한 후 갈색거저리 유충을 전자레인지에서 15분간 건조하였다. 이 후 상기 건조된 유충을 명진기계(참기름기계)를 이용하여 600 kgf/cm2 압력에서 20분간 탈지하였다. 이 후 탈지된 유충을 핀크레샤밀(가려산업)을 이용하여 50 메시(mesh)체에 장착된 상태에서 건식 분쇄하여 유충 분말을 얻었으며, 이렇게 수득된 분말의 수분함량은 5% 수준이었다. A 8-year-old brown duck ( Tenebrio molitor ) larva was purchased from a professional breeding farm and fasted for 2 days. The brown duck larvae which had been completely discharged were washed 2-3 times with water and dried in a microwave oven for 15 minutes . The dried larvae were then degreased at a pressure of 600 kgf / cm < 2 > for 20 minutes using a Myungjin machine (sesame oil machine). Thereafter, the defatted larvae were dry-pulverized in a state of being mounted on a 50-mesh sieve using a pin-crusher (KARI INDUSTRIAL CO., LTD.) To obtain larval larvae. The moisture content of the thus obtained larvae was 5%.
<비교예 5. 롤밀 분쇄하여 얻은 갈색거저리 유충 분말> ≪ Comparative Example 5 < tb > Powdery brown larvae obtained by roll milling &
전문 사육농가에서 8령의 갈색거저리(Tenebrio molitor) 유충을 구입하여 2일간 금식시켜 배설물을 완전히 배출시킨 갈색거저리 유충을 흐르는 물로 2~3회 수세한 후 갈색거저리 유충을 전자레인지에서 15분간 건조하였다. 이 후 롤밀의 롤 회전속도를 150rpm로 하여 상기 건조된 유충을 분쇄하였다. A 8-year-old brown duck ( Tenebrio molitor ) larva was purchased from a professional breeding farm and fasted for 2 days. The brown duck larvae which had been completely discharged were washed 2-3 times with water and dried in a microwave oven for 15 minutes . Thereafter, the dried larva was pulverized at a roll rotation speed of 150 rpm.
이렇게 얻은 갈색거저리 유충의 분말의 사진은 도 2에 나타내었다. 도 2의 사진을 참고하면, 도 1의 분무건조 분말에 비해 도 2의 롤밀 분쇄하여 얻은 분말의 입자가 매우 거칠고 큰 것으로 확인된다. A photograph of the brown gill larva powder thus obtained is shown in Fig. Referring to the photograph of FIG. 2, it is confirmed that the particles of the powder obtained by roll milling in FIG. 2 are very rough and large as compared with the spray dried powder of FIG.
<비교예 6. 동결건조하여 얻은 갈색거저리 유충 분말> ≪ Comparative Example 6 >
전문 사육농가에서 8령의 갈색거저리(Tenebrio molitor) 유충을 구입하여 2일간 금식시켜 배설물을 완전히 배출시킨 갈색거저리 유충을 흐르는 물로 2~3회 수세한 후 갈색거저리 유충을 115℃, 1.3 kgf/cm2 의 고온고압멸균을 5분간 실시하여 멸균한 후 동결건조용 액체질소를 부은 후 급속 동결하여 영하 70℃의 초저온 냉동고에 24시간 동안 동결시킨 후 115℃, 5분 동안 멸균한 후 동결 건조기를 이용하여 건조시켜 수분을 제거하였다. 수분을 제거한 갈색거저리 유충을 믹서로 세절하여 1.4mm 정도의 입자를 갖는 갈색거저리 유충 분말을 제조하였다. Tenebrio molitor larvae were purchased from professional breeding farms and fasted for 2 days. The larvae were washed 2 ~ 3 times with running water. The brown larvae were kept at 115 ℃ and 1.3 kgf / cm 2 was sterilized by high temperature and high pressure for 5 minutes and then poured liquid nitrogen for freeze-drying. After rapid freezing, it was frozen in an ultra-low temperature freezer at 70 ° C for 24 hours and then sterilized at 115 ° C for 5 minutes. Followed by drying to remove moisture. The brown goat larvae having a particle size of about 1.4 mm were prepared by dividing the larvae of the brown goat with moisture removed by a mixer.
<비교예 7. 농축 단계를 수행하지 않고 덱스트린을 첨가하여 제조한 분말> ≪ Comparative Example 7: Powder prepared by adding dextrin without performing the concentration step >
실시예 1에서 얻은 갈색거저리 유충 분쇄물을 이용하여 실시예 2의 방법으로 분말을 제조하되, 진공농축하는 단계를 제외하고, 상기 유충 분쇄물에 바로 글리세린지방산 에스테르와 혼합형 토코페롤을 실시예 2와 동량으로 첨가하고, 덱스트린도 혼합형 토코페롤과 동량으로 추가하였다. 이 후 다음단계를 수행함으로써 분말을 제조하였다. Except that the mixture of the glycerin fatty acid ester and mixed tocopherol was added to the larvae of the larvae in the same amount as in Example 2 except for the step of preparing powder by the method of Example 2 using the brown larch larvae obtained in Example 1, And the same amount of dextrin was added to the mixed type tocopherol. The powder was then prepared by performing the following steps.
이 때 덱스트린은 분무건조를 위한 용액에 첨가되는 부형제로서 사용하였다. 일반적으로 분무건조를 위해서는 분무전의 액상에 덱스트린이 첨가되어야 하는데, 예비 실험으로서 유충 분쇄물에 글리세린지방산 에스테르와 혼합형 토코페롤을 첨가한 뒤 덱스트린을 첨가하지 않고 분무건조를 시도하였지만, 분무액의 상태가 안정적이지 않아 분무건조가 거의 불가능하였다. At this time, dextrin was used as an excipient added to the solution for spray drying. In general, for spray drying, dextrin must be added to the liquid phase of the spraying. As a preliminary experiment, it was tried to spray-dry the dextrin without adding dextrin after adding glycerin fatty acid ester and mixed tocopherol to the larvae of the larvae. It was not stable and spray drying was almost impossible.
<실험예 1. 증자조건에 따른 갈색거저리 유충 분쇄물의 입도특성 확인><Experimental Example 1> Determination of particle size characteristics of brown larch larvae according to growing conditions>
본 발명으로 갈색거저리 유충 분말을 제조하기 위한 중간산물인 실시예 1에서 얻은 갈색거저리 유충의 분쇄물을 50 메시(mesh)의 체로 체별하여 체에 통과한 것을 수거하여 80℃의 열풍건조기에서 6시간 건조하였고, 각 조건에서의 건조된 분말의 함량을 하기 표 1에 나타내었다. 이 때 비교조건으로서 증자단계만 제외한 것의 습식분쇄물도 제조하였다. According to the present invention, the crushed larvae of the larvae obtained in Example 1, which is an intermediate product for producing brown larch larvae, were sieved through a 50-mesh sieve, and they were passed through a sieve and collected in a hot-air drier at 80 ° C for 6 hours Dried, and the content of the dried powder at each condition is shown in Table 1 below. At this time, as a comparative condition, a wet pulverized product except for the increase step was also prepared.
표 1을 참고하면, 증자하지 않은 유충보다는 증자한 유충에서, 증자한 유충의 증자방법별로 습식분쇄물이 50 메시(mesh)에 통과한 양(건조물)은 온도를 가하여 증자한 것에서 많았으며, 그 정도는 가압고온조건에서 증자한 것에서 많았다. 증자시간별로는 증자시간이 길수록 건조물량이 많았으나 경제적인 증자시간은 상압 및 가압고온 조건 모두 60분으로 나타났다. As shown in Table 1, the amount (dried matter) passed through the 50 mesh of the wet pulverized product by the method of increasing the larvae in the larvae grown from the larvae not in the non-expanded larvae was increased by adding the temperature, Was more than that in the case of high pressure. By increasing the amount of time, the amount of dry matter was increased as the time of capital increase was longer, but the economic increase time was 60 minutes in both normal pressure and high temperature conditions.
분쇄물 B (상압, 95℃에서 증자하여 습식분쇄)
Pulverized material B (wet pulverization at atmospheric pressure, at 95 캜)
(1.3kgf/cm2, 121℃에서 증자하여 습식분쇄)Crushed product C
(1.3kgf / cm 2 , wet pulverization at 121 ° C)
<< 실험예Experimental Example 2. 과산화물가 및 2. Peroxide value and 지질산패도Lipid rancidity 조사> Research>
실시예 2와 각 비교예에서 얻은 갈색거저리 유충 분말의 저장기간별 산화안정성을 다음과 같이 조사하였다.Oxidative stability of the brown larch larvae obtained in Example 2 and Comparative Examples was examined as follows.
실험예 2-1. 과산화물가 Experimental Example 2-1. Peroxide value
실시예 2와 각 비교예에서 얻은 갈색거저리 유충 분말 50 g을 상온에 저장하면서 저장 후 0, 7, 14, 21일에 과산화물가를 측정한 결과를 표 2에 나타내었다. 과산화물가 조사는 식품공전방법에 따라 측정하였다. 이를 위해 상온에 표 2의 조건으로 저장보관된 각각의 분말 50 g으로부터 유지를 추출하여 각 5 g에 초산:클로로폼(3:2, v/v) 25 mL를 가하여 혼합하고, KI 포화용액 1 mL를 가하여 교반하였다. 교반 후 암실에 10분간 방치하여 반응시킨 후 물 75 mL를 가하고 1%(v/v) 전분용액을 지시약으로 하여 0.01 N Na2S2O3 용액의 청색이 소실되는 시점을 종말점으로 하여 3회 반복 측정하였다. 이 때 유지 추출은 분말 50g에 n-hexane 200mL을 용매로 하여 상온에서 5시간 동안 진탕 및 방치 후 그 여과액을 진공 농축하여 얻었다. Table 2 shows the results of measuring the peroxide value at 0, 7, 14, and 21 days after storage at room temperature while storing 50 g of the larvae obtained from Example 2 and each comparative example. The peroxide value was measured according to the food revolution method. To this end, the oil was extracted from 50 g of each powder stored at room temperature under the conditions shown in Table 2, 25 mL of acetic acid: chloroform (3: 2, v / v) was added to each 5 g, and KI saturated solution 1 mL was added and stirred. After stirring, the reaction mixture was allowed to stand for 10 minutes, and then 75 mL of water was added. After the end of the disappearance of the blue color of the 0.01 N Na 2 S 2 O 3 solution with the 1% (v / v) And repeatedly measured. At this time, the retentive extraction was obtained by shaking and allowing the filtrate to be concentrated in vacuo in 50 g of powder, using 200 mL of n-hexane as a solvent for 5 hours at room temperature.
분말 종류별 과산화물가는 실시예 2의 분말의 경우, 저장 14일 이후로도 지속적으로 낮게 유지되었으나, 비교예들의 분말은 14일 이후 급격하게 과산화물가가 증가된 것으로 확인된다. The peroxide value of the powder of Example 2 was kept low even after 14 days of storage, but that of the powder of Comparative Example was rapidly increased after 14 days.
실험예Experimental Example 2-2. 2-2. 지질산패도Lipid rancidity
실시예 2와 각 비교예에서 얻은 갈색거저리 유충 분말을 상온에 저장하면서 저장 후 0, 7, 14, 21일에 지질산패도(TBA)를 측정한 결과를 표 3에 나타내었다. 이를 위해 갈색거저리 유충 분말 5 g에 20% trichloroacetic acid 용액 12.5 mL를 첨가하여 14,000 rpm으로 1분간 균질화시키고 증류수를 첨가하여 50 mL로 희석하여 교반한 다음 여과지(Whatman No. 1)에 여과시켜 여과액 5 mL를 2-thiobarbituric acid 용액 5 mL과 혼합하여 실온에서 20시간 방치시킨 후 분광광도계를 사용하여 530 nm에서 흡광도 값을 측정하여 TBA가로 하였다.Table 3 shows the results of measurement of lipid peroxidation (TBA) at 0, 7, 14, and 21 days after storage at room temperature while the brown larch larvae obtained in Example 2 and Comparative Examples were stored at room temperature. To this, 12.5 mL of 20% trichloroacetic acid solution was added to 5 g of brown larch larvae, and the mixture was homogenized at 14,000 rpm for 1 minute, diluted to 50 mL with distilled water, and then filtered through filter paper (Whatman No. 1) 5 mL was mixed with 5 mL of 2-thiobarbituric acid solution and left at room temperature for 20 hours. The absorbance was measured at 530 nm using a spectrophotometer.
분말 종류별 지방산패율은 실시예 2의 분말의 경우, 저장 14일 이후로도 지속적으로 낮게 유지되었으나, 비교예들의 분말은 14일 이후 급격하게 지방산패가 급격하게 증가된 것으로 확인된다. In case of the powder of Example 2, the fat acid ratios were constantly maintained low after 14 days of storage, but the powder of Comparative Examples was rapidly increased after 14 days.
<< 실험예Experimental Example 3. 3. 갈색거저리Brown ass 유충 분말의 품질특성조사> Investigation of Quality Characteristics of Larval Powder>
실시예 2와 각 비교예에서 얻은 갈색거저리 유충 분말의 조지방 및 조단백질 함량과 입도크기 및 분말유동성을 조사한 결과를 표 4에 나타내었다. 분석방법은 공인분석화학자협회(AOAC)의 공정분석법에 준하였다. 즉, 조지방 함량은 ether 추출법, 조단백질 함량은 Kjedahl 질소정량법에 준하여 분석하였다. 분말입도조사는 분말을 50 메시(mesh)의 체로 체별하여 체에 통과된 량과 통과되지 않은 양으로 구분하였다. Table 4 shows the crude fat and crude protein content, particle size and powder fluidity of the brown larch larvae obtained in Example 2 and Comparative Examples. The analytical method was based on the process analysis method of the Association of Certified Analytical Chemists (AOAC). That is, crude fat content was analyzed by ether extraction method and crude protein content was analyzed by Kjedahl nitrogen determination method. Powder particle size was determined by dividing the powder into a 50 mesh sieve and an amount not passed through the sieve.
(50메시 체 통과율)Powder particle size (%)
(50 mesh body throughput)
* 분말의 유동성은 양호 : ◎, 보통 : ○, 매우 불량 : X 로 표시* Fluidity of powder is good: ◎, medium: ○, very poor: marked with X
상기 표 4를 참고하면, 탈지하여 건식분쇄한 비교예 4의 분말은 분말 입도 및 분말의 유동성은 좋으나 조지방함량이 18.36%로 비교예 5나 실시예 2의 분말(35.83, 36.12%)보다 적었다. 롤밀 분쇄하여 얻은 비교예 5의 분말은 조지방함량은 35.83%를 나타냈으나 분말 입도 및 분말의 유동성이 매우 낮았다. 실시예 2의 분말은 조지방함량이 36.12%이었으며 분말 입도 및 분말의 유동성이 좋았다. 한편 농축과정을 수행하지 않고 분무건조한 비교예 7의 분말은 조지방 함량과 조단백 함량에 대한 차이는 많지 않았으며, 분무건조를 위한 부형제로서 덱스트린을 첨가하였음에도 분말입도와 분말유동성이 매우 좋지 않은 것을 알 수 있다. Referring to Table 4, the powder of Comparative Example 4, which was degreased and dry-pulverized, had good powder particle size and powder flowability, but had a crude fat content of 18.36%, which was smaller than those of Comparative Example 5 and Example 2 (35.83 and 36.12%). The powder of Comparative Example 5 obtained by roll milling showed a crude fat content of 35.83%, but the powder particle size and the fluidity of the powder were very low. The powder of Example 2 had a crude fat content of 36.12%, and the powder particle size and the fluidity of the powder were good. On the other hand, the powder of Comparative Example 7, which was spray-dried without performing the concentration process, showed little difference in the crude fat content and the crude protein content, and it was found that the powder particle size and powder fluidity were not very good even though dextrin was added as an excipient for spray drying have.
<제제예 1. 약학적 제제 - 정제>≪ Preparation Example 1 > Pharmaceutical formulation - tablet &
실시예 2에서 얻은 갈색거저리 유충 분말 200g을 락토오스 175.9g, 감자전분 180g 및 콜로이드성 규산 32g과 혼합하였다. 이 혼합물에 10% 젤라틴 용액을 첨가시킨 후, 분쇄해서 14 메쉬체를 통과시켰다. 이것을 건조시키고 여기에 감자전분 160g, 활석 50g 및 스테아린산 마그네슘 5g을 첨가해서 얻은 혼합물을 정제로 만들었다. 200 g of the larval larvae powder obtained in Example 2 was mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.
<제제예 2. 식품 제조><Formulation Example 2: Food Preparation>
제제예 2-1. 조리용 양념의 제조Formulation Example 2-1. Manufacture of cooking seasonings
실시예 2에서 얻은 갈색거저리 유충 분말을 조리용 양념에 1 중량%로 첨가하여 건강 증진용 조리용 양념을 제조하였다.The brown gourd larva powder obtained in Example 2 was added to the cooking seasoning at 1% by weight to prepare a cooking sauce for health promotion.
제제예 2-2. 밀가루 식품의 제조Formulation Example 2-2. Manufacture of flour food products
실시예 2에서 얻은 갈색거저리 유충 분말을 밀가루에 0.1 중량%로 첨가하고, 이 혼합물을 이용하여 빵, 케이크, 쿠키, 크래커 및 면류를 제조하여 건강 증진용 식품을 제조하였다.The brown gill larva powder obtained in Example 2 was added to the wheat flour at 0.1 wt%, and bread, cake, cookies, crackers and noodles were prepared by using the mixture to prepare a health improving food.
제제예 2-3. 스프 및 육즙(gravies)의 제조Preparation Example 2-3. Manufacture of soups and gravies
실시예 2에서 얻은 갈색거저리 유충 분말을 스프 및 육즙에 0.1 중량%로 첨가하여 건강 증진용 수프 및 육즙을 제조하였다.Health enhancing soup and juice were prepared by adding the brown gill larva powder obtained in Example 2 to the soup and juice at 0.1 wt%.
제제예 2-4. 유제품(dairy products)의 제조Formulation Example 2-4. Manufacture of dairy products
실시예 2에서 얻은 갈색거저리 유충 분말을 우유에 0.1 중량%로 첨가하고, 상기 우유를 이용하여 버터 및 아이스크림과 같은 다양한 유제품을 제조하였다.A brown larch larva powder obtained in Example 2 was added to milk in an amount of 0.1 wt%, and various dairy products such as butter and ice cream were prepared using the milk.
제제예 2-5. 야채주스 제조Formulation Example 2-5. Vegetable juice manufacturing
실시예 2에서 얻은 갈색거저리 유충 분말 0.5g을 토마토주스 또는 당근주스 1,000㎖에 가하여 건강 증진용 야채주스를 제조하였다.The vegetable juice for health promotion was prepared by adding 0.5 g of the larvae of the larvae obtained in Example 2 to 1,000 ml of tomato juice or carrot juice.
제제예 2-6. 과일주스 제조Formulation Example 2-6. Manufacture of fruit juice
실시예 2에서 얻은 갈색거저리 유충 분말 0.1g을 사과주스 또는 포도주스 1,000㎖에 가하여 건강 증진용 과일주스를 제조하였다.Health enhancing fruit juice was prepared by adding 0.1 g of the brown larvae obtained in Example 2 to 1,000 ml of apple juice or grape juice.
Claims (9)
(제2단계) 상기 제1단계에서 증자된 갈색거저리 유충을 균질화하고 습식분쇄하여 갈색거저리 유충의 습식분쇄물을 얻는 단계;
(제3단계) 상기 제2단계에서 얻은 습식분쇄물을 농축하여 농축물을 얻는 단계;
(제4단계) 상기 제3단계에서 얻은 농축물에 유화제와 산화안정제를 첨가하고 유화하여 유화물을 얻는 단계; 및,
(제5단계) 상기 제4단계에서 얻은 유화물을 분무건조하는 단계;
를 포함하는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.(Step 1) Increasing the larvae of brownie;
(Step 2) homogenizing and wet pulverizing the brown larvae grown in the first step to obtain a wet pulverized product of brown larvae;
(Step 3) concentrating the wet pulverized product obtained in the second step to obtain a concentrate;
(Step 4) adding an emulsifier and an oxidation stabilizer to the concentrate obtained in the third step and emulsifying to obtain an emulsion; And
(Step 5) spray drying the emulsion obtained in the fourth step;
Wherein the method comprises the steps of: (a) preparing a powder of a larval larva of brown gorilla by using a wet grinding method and a spray drying method.
상기 제1단계의 갈색거저리 유충의 증자는, 갈색거저리 유충을 1~3일간 금식시켜 배설물이 배출된 것에 1~3배 중량의 정제수를 첨가하여, 압력 1.1~1.5kgf/cm2 및 온도 110℃~150℃의 가압고온 조건에서, 30~120분간 수행하는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
Increase of the mealworm larvae of the first stage, to fast, to a mealworm larvae 1-3 days the slurry was added 1-3 times the weight of purified water to the discharge, the pressure 1.1 ~ 1.5kgf / cm 2 and Wherein the mixture is carried out at a high temperature of 110 to 150 DEG C for 30 to 120 minutes.
제2단계의 균질화는 균질기로 800~20000rpm에서 5~15분간 분쇄하여 수행하는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
Wherein the homogenization in the second step is carried out by homogenizing at 800 to 20,000 rpm for 5 to 15 minutes. The method for producing powder of brown larvae using the wet pulverization method and the spray drying method.
상기 제3단계에서 습식분쇄물의 농축은, 최종 농축물의 당도가 20~30 브릭스(brix)가 되도록 수행하는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
Wherein the concentration of the wet pulverized product in the third step is performed such that the sugar content of the final concentrate is 20 to 30 brix.
상기 제4단계에서 유화제로는 글리세린지방산 에스테르, 폴리글리세린 지방산에스테르, 슈가에스텔, 솔비탄 지방산 에스테르, 레시틴, 효소분해 레시틴, 알긴산, 카라기닌 및 구암검으로 이루어진 군에서 선택되는 1종 이상이 포함되는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
As the emulsifier in the fourth step, at least one selected from the group consisting of glycerin fatty acid ester, polyglycerin fatty acid ester, sugar ester, sorbitan fatty acid ester, lecithin, enzyme digesting lecithin, alginic acid, Wherein the powder is produced by a wet grinding method and a spray drying method.
상기 제4단계에서 산화안정제로는 혼합형토코페롤, 녹차추출물분말, 아스코빈산, 아스코빈산 나트륨, 레시틴, 구연산 및 베타카로틴으로 이루어진 군에서 선택되는 1종 이상이 포함되는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
In the fourth step, the oxidative stabilizer includes at least one selected from the group consisting of mixed tocopherols, green tea extract powder, ascorbic acid, sodium ascorbic acid, lecithin, citric acid and beta-carotene. Method for preparing powder of brown larvae using the drying method.
상기 제4단계에서 농축물의 유화는 5~15분간 초음파 처리하여 수행하는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.The method according to claim 1,
Wherein the emulsification of the concentrate is performed by sonication for 5 to 15 minutes in the fourth step, followed by performing the wet pulverization method and the spray drying method.
상기 초음파 처리 전에 1~10분간 균질기를 이용하여 분쇄하여 예비 유화하는 단계가 포함되는 것을 특징으로 하는 습식분쇄법 및 분무건조법을 이용한 갈색거저리 유충의 분말 제조방법.8. The method of claim 7,
Wherein the step of pulverizing and pre-emulsifying the mixture using a homogenizer for 1 to 10 minutes before the ultrasonic treatment comprises a wet grinding method and a spray drying method.
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