KR101793603B1 - Diasome and cosmetic compositions containing the same - Google Patents

Abstract

The present invention relates to a diasome and a cosmetic composition including the same, and more specifically, to the diasome, a nanostructure composed of lecithin, which is a new structure that can structurally penetrate the skin much more stable than conventional liposomes. By adding diamond, sapphire, or ruby powder during the preparation of diasome, a particle form which can structurally penetrate the skin more stable than nanostructures such as conventional liposomes can be materialized, thereby skin absorption rate can be enhanced. In addition, by capturing cosmetic ingredients and the like which have skin regenerating, whitening, or detoxifying effects inside the diasome, the efficacy and effects thereof can be enhanced, and diasome has excellent utility as a cosmetic ingredient.

Description

DIASOME AND COSMETIC COMPOSITIONS CONTAINING THE SAME [0002]

The present invention relates to a diazom and a cosmetic composition comprising the same, and more particularly, to a diazomine which is structurally more stable than a conventional liposome composed of lecithin and can penetrate the skin, and a cosmetic composition .

In the 1960s, it was discovered that phospholipid-based surfactants spontaneously form biomembrane membranes such as biomembrane in the aqueduct media. Liposomes, which are nanostructures, have not only been a model of biomembrane studies, but also target- Many studies have been conducted on the application of the drug as a delivery vehicle in vivo for relief. Liposomes have been studied as sterically stabilized liposomes, fusogenic liposomes, pH sensitive liposomes, temperature sensitive liposomes, and immunoliposomes, and have been found to be useful in the treatment of various diseases including vitamins such as antibodies and folates, growth factors, It may be used as an anticancer agent or a gene carrier by improving the stability by surface modification of the ligand or adding PEG to the surface layer.

Liposome, which is a nano-structure, mainly uses a method of forming a self-assembly body in the manufacturing method. The amphipathic polymer forms a self-assembly in aqueous solution to form polymer micelles at the nanoscale level. The particles stabilize through the steric hindrance or charge repulsion at the surface and have a low critical micelle concentration (CMC). In the liposome particles, various physiologically active substances such as genes, enzymes, and drugs can be collected, and water-soluble surfaces can be variously functionalized to control the circulation time in the body and obtain a targeting effect on tumor cells. Because of the high industrial value of liposomes, the lecithin separation and extraction industry has been developed to increase the purity of lecithin, the main component of liposomes.

Lecithin (phospholipid), which is used to make liposomes, is a class of natural surfactants. Soybean lecithin and egg yolk lecithin are used in the fields of cosmetics, medicines and foods. They have a hydrophilic group and a hydrophobic group structure, and exhibit different surface area activities depending on the species. Traditionally, the separation and extraction of lecithin has mainly used organic solvents such as acetone. Recently, however, the focus has been focused on the separation and extraction of lecithin by an economical method using a non-toxic solvent. Soybean lecithin derived from soybean is mainly obtained as a byproduct of the maintenance and purification process. Egg yolk lecithin is generally extracted by using ethanol or isopropanol for egg yolk.

Thus, liposome-mediated drug delivery has been studied as a means for effective and targeted treatment of the human body, has excellent biocompatibility, is easy to manufacture, and can deliver water-soluble and lipid-soluble drugs, There is a problem that it is physically and chemically unstable. Therefore, it is necessary to study a technology capable of realizing a more stable structure.

Korean Patent Publication No. 10-2017-0031496

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a diabase and a cosmetic composition containing the same, which are structurally more stable than conventional liposomes, There is a purpose.

The present invention relates to a method for producing a diamond powder, comprising 0.01 to 0.5 parts by weight of a gem-powder containing at least one selected from the group consisting of diamond powder, sapphire powder and ruby powder; 5.0-20.0 parts by weight of Lysolecithin; 0.5 to 10.0 parts by weight of hydroxydecyl ubiquinone; 0.5 to 10.0 parts by weight of di-panthenol; 0.01 to 10.0 parts by weight of an oil sealant; 0.01 to 10.0 parts by weight of a peptide; 0.01 to 0.2 parts by weight of a sequestering agent; 0.2 to 2.0 parts by weight of a moisturizer; And 35 to 95 parts by weight of purified water.

Diamond powder and other gem powder

The present invention relates to a method for manufacturing a diamond-like structure by applying a gem-powder containing at least one selected from the group consisting of diamond powder, sapphire powder and ruby powder to a diamond structure, It is possible to implement a particle form that can penetrate. The diazomes have a double-stranded structure such as a liposome.

The gem powder not only contributes to the formation of the diamond structure but also exhibits lifting effect, wrinkle improving effect and whitening effect, which give elasticity to the skin when applied to the skin.

The content of the gem powder may be in the range of 0.01 to 0.5 part by weight. When the content of the gem powder is less than 0.01 part by weight, the expected effect and effect are difficult to be exerted.

Lysolecithin

The above-mentioned razor lecithin is preferably a raw material extracted from soybean (Glycine soja seeded extract), which is purified by high purity and contains 90% or more of lyso lecithin. The content of the above-mentioned risolate is preferably 5 to 20.0 parts by weight. When the amount is less than 5.0 parts by weight, the stability of the structure (diazom) is lowered. When the content is more than 20.0 parts by weight, the structure (diazom) is formed because an excessive force is applied to the physical stirrer for making the structure (diazom) It can be an obstruction.

The above-mentioned cholesterol lecithin has a single structure with a small number of units, and conically forms a very dense double structure.

Hydroxydecyl ubiquinone

The content of the hydroxydecyl ubiquinone is preferably 0.5 to 10.0 parts by weight. If the content is less than 0.5 parts by weight, the effect on skin inflammation is deteriorated. If the content is more than 10.0 parts by weight, it is difficult to expect further increase of the efficacy.

Di-panthenol

The content of the dipanthenol is preferably 0.5 to 10.0 parts by weight. If the content is less than 0.5 part by weight, the skin regeneration effect is lowered. If the content is more than 10.0 parts by weight, it is difficult to expect an increase in the additional efficacy.

Oil sealant

The oil sealant may be selected from the group consisting of Caprylic / Capric Triglyceride, Squalane, Argania Spinosa Kernel Oil, Tocopheryl Acetate, Macadamia Oil Integrifolia Seed Oil, Helianthus Annuus Seed oil, and Rosmarinus Officinalis Leaf Extract.

The oil sealant is preferably 0.01 to 10.0 parts by weight. When the oil sealant is added in the above range, it contributes not only to the formation of the structure but also to the skin regeneration and antioxidative effect when used on the skin.

 However, when the oil sealant content is less than 0.01 part by weight, the structure (diamox) is not formed properly. When the sealant content is more than 10.0 parts by weight, the particle size of the nanostructure is increased and the stability is lowered.

Peptides, sequestering agents and moisturizers

The above-mentioned peptides, sequestering agents and humectants can be used according to the degree of effect required by selecting each of the conventional components known in the art to which the present invention belongs, and the kind and content thereof are not particularly limited.

The peptide may be selected from the group consisting of hexapeptide-42, lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, , ≪ / RTI >

As the metal ion sequestering agent, disodium EDTA is preferably used. The metal ion blocking agent may be included in an amount of 0.01 to 0.2 parts by weight.

As the humectant, 1,2-hexanediol is preferably used. The moisturizing agent may be included in an amount of 0.2 to 2.0 parts by weight.

The average diameters of the diamonds may be between 95 and 99 nm. When the diameter is less than 95 nm, it may be difficult to sufficiently contain cosmetic ingredients in the diamond, and when the diameter exceeds 99 nm, the skin absorption rate is lowered.

The present invention also provides a cosmetic composition comprising the above-mentioned diazom.

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The cosmetic composition containing diazomine may be used in the manufacture of cosmetics of various formulations. Specifically, the cosmetic composition may be formulated into a formulation selected from the group consisting of skins, essences, lotions, creams and mask packs.

According to the present invention, by adding diamond, sapphire, or ruby powder during the production of the structure (diazom), it is possible to realize a particle shape that can penetrate the skin more structurally and more stably than nano structures such as existing liposomes, Can be improved. In addition, cosmetic ingredients such as skin regeneration, whitening, or detoxifying effect can be collected inside the structure, thereby improving its efficacy and effectiveness, and thus being useful as a cosmetic raw material.

Fig. 1 is a photograph showing the material produced according to Example 1 and Comparative Examples 1 and 2. Fig.
2 is a graph showing the results of particle size distribution analysis for Example 1. Fig.
3 is a graph showing the results of particle size distribution analysis for Comparative Example 2. Fig.
Fig. 4 is a graph showing collagen synthesis ability of Example 1 and Comparative Example 2; Fig.

Hereinafter, the present invention will be described in more detail with reference to Examples. The objects, features and advantages of the present invention will be readily understood through the following examples. The present invention is not limited to the embodiments described herein, but may be embodied in other forms. The embodiments described herein are provided to enable those skilled in the art to fully understand the spirit of the present invention. Therefore, the present invention should not be limited by the following examples.

Example 1: Preparation of diamoxine

The components shown in Table 1 were stirred at room temperature for 1 hour to prepare a diar structure (hereinafter, referred to as 'diamond') (see FIG. 1).

number Classification ingredient Comparative Example 1
(Parts by weight) Comparative Example 2
(Parts by weight) Example 1
(Parts by weight) One * Soybean extract Soybean extract
(Glycinesoja (soybean) seedextract) 10.0 10.0 10.0 2 Beauty ingredient extract
* Hydroxydecyl ubiquinone 0 4.0 4.0 3 * Di Panthenol 0 4.0 4.0 4 Gem powder * Diamonds, rubies, sapphires
(Mixed at a weight ratio of 1: 0.5: 0.5) 0 0 0.1 5 Peptides
Hexapeptide-42 (1 ppm) 0 1.0 1.0 6 17 amino acid complexes 0 1.0 1.0 7 Oil
Sealant


Caprylic / Capric Triglyceride (Caprylic / Capric Triglyceride) 0 4.5 4.5 8 Squalane 0 0.2 0.2 9 Argan Oil 0 0.1 0.1 10 Tocopheryl acetate
(TocopherylAcetate) 0 0.1 0.1 11 Metal ion sequestrant Disodium EDTA < RTI ID = 0.0 > 0 0.1 0.1 12 Moisturizer 1,2-Hexanediol 0 2.0 2.0 13 Purified water Purified water 90.0 73.0 72.9 * Hydroxydecyl ubiquinone: (Product name: Idebenone, manufacturer: Biobutec)
* Dipantanol: (product name: D-PANTENOL, manufactured by DSM (UK))
* Diamond, Ruby, Sapphire Powder: (Product Name: Jewelry Complex, Manufacturer: Moakhem)

Comparative Examples 1 and 2

A structure was prepared in the same manner as in Example 1 (see Table 1, Fig. 1).

Example 2 and Comparative Example 3: Preparation of cosmetic formulations

Various formulations of cosmetics were prepared with the compositions of Table 2 and Table 3. Each formulation of cosmetics was prepared by stirring the components listed in the table below.

The reason why the fruit acids were added to the raw materials included in the formulations of Tables 2 to 3 below was added in order to test whether or not skin irritation was observed in Experimental Example 2 described below.

ingredient
Example 2 (parts by weight) T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 90.03 87.13 87.47 76.83 65.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 Example 1 2.0 2.0 2.0 2.0 2.0

ingredient
Comparative Example 3 (parts by weight) S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 92.03 89.13 89.47 78.83 67.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 Comparative Example 2 2.0 2.0 2.0 2.0 2.0

Experimental Example 1: Characterization of Particles

The characteristics of the particles prepared according to Example 1 and Comparative Example 2 were analyzed and are shown in Table 5. Particle size (average diameter) and particle size distribution were analyzed using light scattering measurement equipment. The light scattering measurement device is NANO-ZS90 (manufacturer: Malvern Korea), which is used for characterizing nanoparticles and colloids as a particle and molecular size, zeta potential and molecular weight measurement product. The measurement conditions are shown in Table 4.

Example 1 Comparative Example 2 Temperature  19.8 ° C 20.0 DEG C  Solvent Name water water Refractive Index 1.3318 1.3318

Example 1 Comparative Example 2 Average diameter of particles  97. 3 nm 102.2 nm

As shown in Table 5, it was confirmed that the diameters of the diamonds according to Example 1 were smaller, and a more uniform particle size distribution was exhibited. As a result, the particles were structurally more stable than the particles of Comparative Example 1 (Fig. 2).

Experimental Example 2: Sensory evaluation

The following sensory evaluations were performed to evaluate the skin moisturizing feeling, feeling of use, feeling of firmness, improvement of skin condition, feeling after use, and skin irritation effect of the cosmetic formulations prepared according to Example 2 and Comparative Example 3.

First, 30 women who were between 21 and 40 years old were divided into three groups of 10 people. After washing the face of the subject to be tested cleanly, 0.1 g to 0.2 g of the content of Example 2 was uniformly applied to the face half and 0.1 g to 0.2 g of the contents of Comparative Example 2 was uniformly applied to the other half face Respectively. For each formulation, each 7-day trial was discontinued for one week, followed by 7 days. After each 7 days, the skin was moisturized, feeling good, feeling of firmness, improvement of skin condition, feeling after use, and skin irritation were evaluated for sensory evaluation, and the results are shown in Tables 6 to 7 below.

When the effect felt at this time was excellent, it was evaluated as 5 points, and when the effect was not felt at all, it was evaluated as 6 points.

Evaluation items
Example 2 T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Moisturizing 4.0 3.5 4.5 4.5 4.0 Feeling 4.0 4.0 4.0 4.0 4.0 A sense of urgency 4.5 4.5 4.5 4.0 4.5 Improved skin condition 4.0 4.0 4.0 4.0 4.0 After use feeling 4.0 3.5 4.0 4.0 4.0 Skin irritation 0 0 0 0 0 Skin clear 4.5 4.0 4.5 4.5 4.5

Evaluation items
Comparative Example 3 S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Moisturizing 3.5 3.5 3.5 3.5 3.0 Feeling 3.5 4.0 3.5 3.5 3.5 A sense of urgency 3.5 3.5 4.0 3.5 3.5 Improved skin condition 3.5 3.5 3.5 3.5 3.5 After use feeling 4.0 3.5 3.5 3.5 3.5 Skin irritation 0 0 0 0 0 Skin clear 3.5 3.0 3.0 3.0 3.5

As shown in the above-mentioned [Table 6] and [Table 7], when the formulation of Example 2 was used, there was no irritation to the skin, and in addition to the feeling of moisturizing feeling, feeling of use, .

Experimental Example  3: Evaluation of collagen synthesis ability

In order to evaluate the collagen synthesis ability according to Example 1 and Comparative Example 2, this experiment was carried out in order to confirm the degree of expression of Procollagen C-endopeptidase enhancer: PCOLCE, which is a marker involved in collagen synthesis in human fibroblast (CCD986sk) Specifically human fibroblasts were incubated with 100 mM / 60.1 cm < ² > (100 mM) in combination with Dulbecco's Modified Eagle's Medium (DMEM), 10% Fetal bovine serum (FBS), 1% Antibiotic- Antimycotic (GIBCO, Cat No.15240-062) culture dish at 37 ° C and 5% CO ₂ . When human cells were confluent at 100% or more, they were cultured in 96-well plates at 1 × 10 ⁴ cells / well for 24 hours. Thereafter, the medium was replaced with DMEM free medium (medium lacking FBS), and the test sample according to Example 1 and Comparative Example 2 was treated with a cytotoxic concentration of 0.001%. Then, the culture was further cultured for 24 hours under the cell culture condition, and the effect of PCOLCE on the expression of PCOLCE was analyzed. Real-time PCR was performed for the test method.

Changes in the expression of PCOLCE gene related to procollagen synthesis by the samples prepared according to Example 1 and Comparative Example 2 in human fibroblast cell line CCD986 were observed. PCOLCE is a gene that directly affects the collagen synthesis process, and the effect of each sample on the expression of PCOLCE and the effect of increasing collagen formation is analyzed. As a result, it was confirmed that PCOLCE expression was increased by about 25% in Comparative Example 2 and about 50% in Example 1, compared with the negative control. These results show that the gem powder (diamond: ruby: sapphire = 1: 0.5: 0.5) not only affects the collagen synthesis, but also the ability to synthesize collagen when forming a diamond structure together with cosmetic ingredients, peptides, (Fig. 4).

Claims (4)

0.01 to 0.5 parts by weight of a gem powder containing at least one selected from the group consisting of diamond powder, sapphire powder and ruby powder;
5.0-20.0 parts by weight of Lysolecithin;
0.5 to 10.0 parts by weight of hydroxydecyl ubiquinone;
0.5 to 10.0 parts by weight of di-panthenol;
0.01 to 10.0 parts by weight of an oil sealant;
0.01 to 10.0 parts by weight of a peptide;
0.01 to 0.2 parts by weight of a sequestering agent;
0.2 to 2.0 parts by weight of a moisturizer; And
35 to 95 parts by weight of purified water,
Wherein the diamonds have an average diameter of 95 to 99 nm.
delete A cosmetic composition comprising the diazo of claim 1.
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