KR101830269B1 - Liposome comprising kummerowia striata and cosmetic compositions containing the same - Google Patents

Abstract

The present invention relates to a liposome containing Kummerowia striata and to a cosmetic composition containing the same, and more particularly, to a liposome containing Kummerowia striata, a novel structure capable of penetrating into the skin more structurally than general liposome, which is a nano structure composed of lecithin, and to a cosmetic composition containing the same. In the present invention, extracts of Kummerowia striata, Calendula officinalis, yeast, and upland cotton are applied to the manufacture of liposome, so it is possible to realize a particle form which can penetrate the skin more structurally and more stably than nano structures such as conventional liposomes, thereby significantly improving a whitening effect of the existing Kummerowia striata extract.

Description

TECHNICAL FIELD [0001] The present invention relates to a knot full-containing liposome and a cosmetic composition containing the knot full-

The present invention relates to a knot full-containing liposome and a cosmetic composition comprising the same, and more particularly to a knot full-containing liposome, which is a new structure capable of penetrating the skin structurally more stably than a general liposome composed of lecithin, To a cosmetic composition containing the same.

In the 1960s, it was discovered that phospholipid-based surfactants spontaneously form biomembrane membranes such as biomembrane in the aqueduct media. Liposomes, which are nanostructures, have not only been a model of biomembrane studies, but also target- Many studies have been conducted on the application of the drug as a delivery vehicle in vivo for relief. Liposomes have been studied as sterically stabilized liposomes, fusogenic liposomes, pH sensitive liposomes, temperature sensitive liposomes, and immunoliposomes, and have been found to be useful in the treatment of various diseases including vitamins such as antibodies and folates, growth factors, It may be used as an anticancer agent or a gene carrier by improving the stability by surface modification of the ligand or adding PEG to the surface layer.

Liposome, which is a nano-structure, mainly uses a method of forming a self-assembly body in the manufacturing method. The amphipathic polymer forms a self-assembly in aqueous solution to form polymer micelles at the nanoscale level. The particles stabilize through the steric hindrance or charge repulsion at the surface and have a low critical micelle concentration (CMC). In the liposome particles, various physiologically active substances such as genes, enzymes, and drugs can be collected, and water-soluble surfaces can be variously functionalized to control the circulation time in the body and obtain a targeting effect on tumor cells. Because of the high industrial value of liposomes, the lecithin separation and extraction industry has been developed to increase the purity of lecithin, the main component of liposomes.

Lecithin (phospholipid), which is used to make liposomes, is a class of natural surfactants. Soybean lecithin and egg yolk lecithin are used in the fields of cosmetics, medicines and foods. They have a hydrophilic group and a hydrophobic group structure, and exhibit different surface area activities depending on the species. Traditionally, the separation and extraction of lecithin has mainly used organic solvents such as acetone. Recently, however, the focus has been focused on the separation and extraction of lecithin by an economical method using a non-toxic solvent. Soybean lecithin derived from soybean is mainly obtained as a byproduct of the maintenance and purification process. Egg yolk lecithin is generally extracted by using ethanol or isopropanol for egg yolk.

Thus, liposome-mediated drug delivery has been studied as a means for effective and targeted treatment of the human body, has excellent biocompatibility, and is easy to manufacture and can transport water-soluble and lipid-soluble drugs. However, There is a problem that it is physically and chemically unstable. Therefore, it is necessary to study a technology capable of realizing a more stable structure.

Korean Patent Publication No. 10-2017-0031496

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a novel structure capable of penetrating into skin more structurally than general liposome, The purpose is to provide.

The present invention relates to a composition comprising 2.0 to 5.0 parts by weight of a knot grass extract; 5.0-20.0 parts by weight of Lysolecithin; 0.5-10.0 parts by weight of Karen dulcis extract; Yeast extract 0.5 to 10.0; 0.5-10.0 parts by weight of ground extract; 0.01 to 10.0 parts by weight of an oil sealant; 0.01 to 10.0 parts by weight of a peptide; 0.01 to 0.2 parts by weight of a sequestering agent; 0.2 to 2.0 parts by weight of a moisturizer; And 35 to 95 parts by weight of purified water.

The knot pool-containing liposome may further comprise Ascorbic Acid. In this case, the content may be 0.5 to 10.0 parts by weight.

Hereinafter, in the specification of the present invention, the knot pool-containing liposome constituted as described above is defined as "queosa. &Quot;

Knot grass extract, Karen dili extract, yeast extract and ground extract

By applying knot grass extract, Karen dulcimer extract, yeast extract and ground extract to the production of liposomes, it is possible to realize a particle form that can penetrate the skin structurally more stably than conventional nano-structures such as liposomes. Therefore, the whitening effect of the existing knot grass extract can be significantly improved.

Wherein the knot grass extract, the Karen dulcimer extract, the yeast extract or the ground extract, the knot grass, the Karen dulcis, the yeast or the ground surface are dissolved in water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, 3-butylene glycol, and the like.

Lysolecithin

The above-mentioned razor lecithin is preferably a raw material extracted from soybean (Glycine soja seeded extract), which is purified by high purity and contains 90% or more of lyso lecithin. The content of the above-mentioned risolate is preferably 5 to 20.0 parts by weight. When the content is less than 5.0 parts by weight, the stability of the structure (cucumber) is lowered. When the content is more than 20.0 parts by weight, excessive force is applied to the physical stirrer to make the structure (cucumber) .

The above-mentioned cholesterol lecithin has a single structure with a small number of units, and conically forms a very dense double structure.

Oil sealant

The oil sealant may be selected from the group consisting of Caprylic / Capric Triglyceride, Squalane, Argania Spinosa Kernel Oil, Tocopheryl Acetate, Macadamia Oil Integrifolia Seed Oil, Helianthus Annuus Seed oil, and Rosmarinus Officinalis Leaf Extract.

The oil sealant is preferably 0.01 to 10.0 parts by weight. When the oil sealant is added in the above range, it contributes not only to the formation of the structure but also to the skin regeneration and antioxidative effect when used on the skin.

 However, when the oil sealant content is less than 0.01 part by weight, the structure (cucumber) is not formed properly, and when the sealant content is more than 10.0 parts by weight, the particle size of the nanostructure becomes excessively large and stability becomes poor.

Peptides, sequestering agents and moisturizers

The above-mentioned peptides, sequestering agents and humectants can be used according to the degree of effect required by selecting each of the conventional components known in the art to which the present invention belongs, and the kind and content thereof are not particularly limited.

The peptide may be selected from the group consisting of hexapeptide-42, lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, , ≪ / RTI >

As the metal ion sequestering agent, disodium EDTA is preferably used. The metal ion blocking agent may be included in an amount of 0.01 to 0.2 parts by weight.

As the humectant, 1,2-hexanediol is preferably used. The moisturizing agent may be included in an amount of 0.2 to 2.0 parts by weight.

The average diameter of the cinnamon may be 124 to 127 nm. When the diameter is less than 124 nm, it is difficult to realize an expected whitening effect, and when the particle diameter exceeds 127 nm, the skin absorption rate is lowered.

The present invention also provides a cosmetic composition comprising the cucumber.

The cucumber may be contained in an amount of 0.001 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition. When the content is less than 0.001 part by weight, it is difficult to realize the expected effect, and when the content is more than 20 parts by weight, it is difficult to expect further increase of the efficacy.

The cosmetic composition containing the cucumber may be used in the manufacture of cosmetics of various formulations, and may be formulated into a formulation selected from the group consisting of skins, essences, lotions, creams and mask packs.

The present invention can be applied to the production of liposomes by applying knot grass extract, Karen dulcis extract, yeast extract and ground extract to form particles that can penetrate the skin more structurally more structurally than conventional nano-structures such as liposomes. The whitening effect of the existing knot grass extract can be significantly improved.

FIG. 1A is a graph showing the results of particle size distribution analysis for Example 1. FIG.
1B is a graph showing the results of particle size distribution analysis for Comparative Example 2. FIG.
2A is an electron microscope image (scale bar 500 nm) showing the particle size distribution for Example 1. Fig.
FIG. 2B is an electron microscope image (scale bar 2 μm) showing the particle size distribution for Comparative Example 2. FIG.
FIG. 3 is a graph showing the results of analysis of the melanin production inhibitory effect against Example 1. FIG.
4 is a graph showing the results of an analysis of melanin production inhibitory activity against Comparative Example 2. Fig.

Hereinafter, the present invention will be described in more detail with reference to Examples. The objects, features and advantages of the present invention will be readily understood through the following examples. The present invention is not limited to the embodiments described herein, but may be embodied in other forms. The embodiments described herein are provided to enable those skilled in the art to fully understand the spirit of the present invention. Therefore, the present invention should not be limited by the following examples.

Example 1: Preparation of cucumber (knot pool-containing liposome)

The components shown in Table 1 were stirred at room temperature for 1 hour to prepare jujube (knot pool-containing liposome) (see Fig. 1).

number Classification ingredient Comparative Example 1
(Parts by weight) Comparative Example 2
(Parts by weight) Example 1
(Parts by weight) One * Soybean extract Soybean extract
(Glycinesoja (soybean) seedextract) 10.0 10.0 10.0 2 Beauty ingredient extract



Knot grass extract 0 5.0 5.0 3 * Karen dulberry extract 0 0 4.0 4 * Yeast extract 0 0 4.0 5 * Ground extract 0 0 4.0 6 7 Peptides
Hexapeptide-42 (1 ppm) 0 1.0 1.0 8 * Amino Acid Complex 0 1.0 1.0 9 Oil
Sealant


Caprylic / Capric Triglyceride (Caprylic / Capric Triglyceride) 0 4.5 4.5 10 Squalane 0 0.2 0.2 11 Argan Oil 0 0.1 0.1 12 Tocopheryl acetate
(TocopherylAcetate) 0 0.1 0.1 13 Metal ion sequestrant Disodium EDTA < RTI ID = 0.0 > 0 0.1 0.1 14 Moisturizer 1,2-Hexanediol 0 2.0 2.0 15 Purified water Purified water 90.0 76.0 64.0 * Soybean extract: (Product name: Lysofix, manufacturer: KEMIN (USA))
* Karen dulcis extract: (product name: epigenomyl, manufacturer: SILAB (France))
* Yeast extract: (Product name: Celldetox, manufacturer: SILAB (France))
* Land extract: (Product name: Heliomodulin, Manufacturer: SILAB (France))
* Amino Acid Complex (17 kinds of amino acid complex extract, manufacturer, Bioland)

In the composition of Table 1 above, 5 l of 80% ethanol aqueous solution was added to 1 kg of the knot grass washed, reflex-extracted three times, and then immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The filtrate was extracted five times with 1 liter of ether to remove the pigment. And extracted three times with 500 ml. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract. The extract was dissolved in a small amount of methanol, and a large amount of ethyl acetate was added thereto. The resultant precipitate was dried to obtain 200g of knot grass extract .

In the present embodiment, commercially available products such as soybean extract, Karen dulcite extract, yeast extract and ground surface extract were used, but it is also possible to prepare an extract by using the same method as the knot full extract.

Comparative Example  1, 2

A structure was prepared in the same manner as in Example 1 (Table 1).

Example 2 and Comparative Example 3: Preparation of cosmetic formulations

Various formulations of cosmetics were prepared with the compositions of Table 2 and Table 3. Each formulation of cosmetics was prepared by stirring the components listed in the table below.

The reason why the fruit acids were added to the raw materials included in the formulations of Tables 2 to 3 below was added in order to test whether or not skin irritation was observed in Experimental Example 2 described below.

ingredient
Example 2 (parts by weight) T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 90.03 87.13 87.47 76.83 65.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 Example 1 2.0 2.0 2.0 2.0 2.0

ingredient Comparative Example 3 (parts by weight) S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 92.03 89.13 89.47 78.83 67.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 Comparative Example 2 2.0 2.0 2.0 2.0 2.0

Experimental Example  1: Characterization of particles

The characteristics of the particles prepared according to Example 1 and Comparative Example 2 were analyzed and shown in Figs. 1, 2 and 5. Particle size (average diameter) and particle size distribution were analyzed using light scattering measurement equipment. The light scattering measurement device is NANO-ZS90 (manufacturer: Malvern Korea), which is used for characterizing nanoparticles and colloids as a particle and molecular size, zeta potential and molecular weight measurement product. The measurement conditions are shown in Table 4.

Example 1 Comparative Example 2 Temperature  23.0 DEG C 22.9 ° C  Solvent Name water water Refractive Index 1.3315 1.3315

Example 1 Comparative Example 2 Average diameter of particles  125.5 nm 150.6 nm

As shown in Table 5, it was confirmed that the average diameter of the cupose according to Example 1 was smaller than that of Comparative Example 2, so that it could be absorbed into the skin structurally more stably than the particles of Comparative Example 2. Further, as shown in Fig. 2, it was confirmed that the cucumber according to Example 1 (see Fig. 2a) exhibited a very uniform particle size distribution as compared with Comparative Example 2 (see Fig. 2b) .

Experimental Example 2: Sensory evaluation

The following sensory evaluation was performed to evaluate skin moisturizing feeling, feeling of use, feeling of firmness, improvement of skin condition, feeling after use, and skin irritation effect of cosmetic formulations prepared according to Example 2 and Comparative Example 3.

First, 30 women who were between 21 and 40 years old were divided into three groups of 10 people. 0.1 g to 0.2 g of the contents of Example 2 were uniformly applied to the half of the face of the subject and 0.1 g to 0.2 g of the contents of Comparative Example 3 were evenly applied to the other half of the face of the opposite face Respectively. For each formulation, each 7-day trial was discontinued for one week, followed by 7 days. After each 7 days, the skin was moisturized, feeling good, feeling of firmness, improvement of skin condition, feeling after use, and skin irritation were evaluated for sensory evaluation, and the results are shown in Tables 6 to 7 below.

When the effect felt at this time was excellent, it was evaluated as 5 points, and when the effect was not felt at all, it was evaluated as 6 points.

Evaluation items Example 2 T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Moisturizing 4.5 4.0 4.5 5.0 4.5 Feeling 4.5 4.5 4.0 4.0 4.0 A sense of urgency 4.5 4.5 5.0 4.0 4.5 Improved skin condition 4.0 4.0 4.0 4.0 4.0 After use feeling 4.0 4.0 4.5 4.0 4.0 Skin irritation 0 0 0 0 0 Skin clear 4.5 4.0 5.0 4.5 4.5

Evaluation items Comparative Example 3 S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Moisturizing 3.5 3.5 3.0 3.5 3.0 Feeling 3.5 3.5 3.0 3.5 3.5 A sense of urgency 3.5 3.5 3.5 3.5 3.5 Improved skin condition 3.5 3.5 3.5 3.5 3.5 After use feeling 4.0 3.0 3.5 3.5 3.5 Skin irritation 0 0 0 0 0 Skin clear 3.0 3.0 3.5 3.0 3.0

As shown in [Table 6] and [Table 7], when the formulation of Example 2 was used, there was no irritation to the skin, and in the case of Comparative Example 3 in terms of moisturizing feeling, feeling of use, Respectively.

Experimental Example 3: Evaluation of inhibitory effect on melanin formation (in vitro whitening effectiveness evaluation)

In order to measure the effect of inhibiting the melanin biosynthesis in the mouse-derived B16F1 melanoma, the liposome prepared in Example 1 was tested as follows. Specifically, B16F1 (mouse melanoma cell) cells were cultured in DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% of bovine serum and 1% of antibiotics, This was dispensed into a 6-well plate and then cultured at 37 ° C and 5% CO ₂ for 24 hours. After 24 hours of culture, the medium was removed and α-MSH (melanocyte stimulating hormone) was added to the wells, and the liposomes of Example 1 and Comparative Example 2 were added to the wells together. As a positive control, arbutin at a concentration of 400 μg / ml and kojic acid at a concentration of 400 μg / ml were used. After culturing for 72 hours, the amount of melanin produced in each plate was measured by removing the remaining culture medium from the plate and washing with 1 ㅧ PBS (phosphate buffered saline). After trypsin was treated in each well and cells were removed, the culture was treated in each well and transferred to a 2 ml microtube. After centrifugation at 10,000 rpm for several minutes, the culture medium in the tube was removed, treated with PBS, and then centrifuged and removed. The PBS was then removed and dried. The cells were treated with 1N NaOH (10% DMSO) and reacted at 70 ° C for several minutes with the lid closed. The results were analyzed and shown in FIG. 3 and FIG. As shown in Fig. 3 and Fig. 4, it was confirmed that the production of melanin was remarkably inhibited in the case of Example 1.

Claims (5)

2.0 to 5.0 parts by weight of knot grass extract;
5.0-20.0 parts by weight of Lysolecithin;
0.5-10.0 parts by weight of Karen dulcis extract;
Yeast extract 0.5 to 10.0;
0.5-10.0 parts by weight of ground extract;
0.01 to 10.0 parts by weight of occlusive oil;
0.01 to 10.0 parts by weight of a peptide; 0.01 to 0.2 parts by weight of a sequestering agent;
0.2 to 2.0 parts by weight of a moisturizer; And
35 to 95 parts by weight of purified water.
The method according to claim 1,
Wherein the liposome further comprises an ascorbic acid.
The method according to claim 1,
Wherein the knot pool-containing liposome has an average diameter of 124 to 127 nm.
A cosmetic composition comprising the knot full-containing liposome of claim 1.
The method of claim 4,
Wherein the knot pool-containing liposome is contained in an amount of 0.001 to 20 parts by weight based on 100 parts by weight of the cosmetic composition.

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