KR101511425B1 - Nano-composition - Google Patents

Abstract

The present invention relates to a nanostructure comprising: 5.0 to 20.0% by weight of Lysolecithin; 0.5 to 10.0% by weight of hydrolyzed pea (Hydrolyzed Pea) as a hydrolyzed soybean raw material; 0.5 to 10.0% by weight of Boehmia duffusa root extract, a natural product of cosmetic ingredients; 0.01 to 10.0% by weight aqueous solution of a peptide; 0.01 to 0.05% by weight of a sequestering agent; 0.01 to 10.0% by weight of an oil sealant; 0.2 to 2.0% by weight of a moisturizer; And purified water in a ratio of 37.95 to 93.77% by weight.
In the present invention thus constituted, nanostructures prepared using a natural substance composed of a ribofosperitide or a lysozyme extracted from a soybean extract are well formed of nanoparticles and are considered to be composed of a bilayer such as liposome. In addition, it can be seen that when the cosmetic ingredient having the skin synthesis or anti-inflammatory effect is contained in the nanostructure, the efficacy is more excellent. This is a cosmetic nanostructure and is effective for skin-beauty as a biocompatible raw material for cosmetics which is excellent in collagen synthesis and anti-inflammatory effect.

Description

Nanostructure {NANO-COMPOSITION}

The present invention relates to a nanostructure prepared by using an extract made of a soybean-extracted Lysophospholipid or Lysolecithin. More specifically, the present invention relates to a nanostructure which is structurally more stable than a general liposome comprising a nanostructure comprising lecithin A method of manufacturing a new nanostructure capable of penetrating into the skin, and a method of collecting the cosmetic ingredients composed of hydrogenated pea extract, sunflower seed oil and rosemary leaf extract, which are effective for skin regeneration, into a nanostructure, The present invention relates to a nanostructure having an anti-inflammatory effect.

Although the history of colloidal science began with the study of the color of particles since Roman times, Faraday's study of the color of colloidal gold particles is an academic discipline. In general, colloids have been understood to have a finely divided particle state. These particle states can create a large surface area, so that the interface and the surface created by the transition state between phases, the high-order structure in the solution such as micelles, liquid crystals and nanostructures, liposomes, non-equilibrium dispersants such as emulsions, Level ultra thin film.

Understanding colloids and interfacial chemistry helps to understand particles such as liposomes, which are nanostructures. Colloids are large systems with a large surface area and surface energy, and they range in size from approximately 10 nm to 1 μm. As the particle size decreases, the surface area per unit mass increases, not only the interfacial energy increases but also the force acting between the particles.

Here, the interfacial energy (ΔG) is expressed as ΔG = d (γA) at a constant temperature and pressure, where A is the interface area and γ is the surface tension. That is, in a nanometer-sized system, the specific energy of the interfacial energy becomes very large, which is different from a uniform bulk phase. According to the law of thermodynamics, the entire system must be arranged so that its free energy is minimum. For example, when the amount of emulsifier is small in the emulsion, the interfacial tension is high and the particle size is large. When the amount of the emulsifier is large, the interfacial tension is decreased and the particle size is also decreased. According to the method using the interfacial energy, the active ingredient of various cosmetics can be contributed to make it easy to deliver deeply into the skin.

In the 1960s, it was discovered that phospholipid-based surfactants spontaneously form biomembrane membranes such as biomembrane in the aqueduct media. Liposomes, which are nanostructures, have not only been a model of biomembrane studies, but also target- Many studies have been conducted on the application of the drug as a delivery vehicle in vivo for relief. Liposomes have been studied as sterically stabilized liposomes, fusogenic liposomes, pH sensitive liposomes, temperature sensitive liposomes, and immunoliposomes, and have been found to be useful in the treatment of various diseases including vitamins such as antibodies and folates, growth factors, It may be used as an anticancer agent or a gene carrier by improving the stability by surface modification of the ligand or adding PEG to the surface layer.

Liposome, which is a nano-structure, mainly uses a method of forming a self-assembly body in the manufacturing method. The amphipathic polymer forms a self-assembly in aqueous solution to form polymer micelles at the nanoscale level. The particles stabilize through the steric hindrance or charge repulsion at the surface and have a low critical micelle concentration (CMC). In the liposome particles, various physiologically active substances such as genes, enzymes, and drugs can be collected, and water-soluble surfaces can be variously functionalized to control the circulation time in the body and obtain a targeting effect on tumor cells. Since the liposome has a high industrial value, the lecithin separating and extracting industry has been developed to enhance the purity of lecithin, which is the main component of the liposome.

Lecithin (phospholipid), which is used to make liposomes, is a class of natural surfactants. Soybean lecithin and egg yolk lecithin are used in the fields of cosmetics, medicines and foods. They have a hydrophilic group and a hydrophobic group structure, and exhibit different surface area activities depending on the species. Traditionally, the separation and extraction of lecithin has mainly used organic solvents such as acetone. Recently, however, the focus has been focused on the separation and extraction of lecithin by an economical method using a non-toxic solvent. Soybean lecithin derived from soybean is mainly obtained as a byproduct of the maintenance and purification process. Egg yolk lecithin is generally extracted by using ethanol or isopropanol for egg yolk.

Thus, liposome-mediated drug delivery has been studied as a means for effective and targeted treatment of the human body, has excellent biocompatibility, and is easy to manufacture and can transport water-soluble and lipid-soluble drugs. However, It is physically and chemically unstable. Therefore, it has disadvantages such as aggregation, fusion, hydrolysis of phospholipid, oxidation and leakage of inclusion drug when dispersed and stored in an aqueous solution.

Lysetecin (lysolecithin or lysophospholipid) was modified by hydrolysis of lecithin with phospholipase to compensate for the oxidative capacity of the unsaturated acyl group, which is a weak point of lecithin, and the hydrolytic capacity of the hydrophilic part. It is a natural surfactant like lecithin and has excellent biocompatibility And is used in pharmaceuticals, pharmaceuticals, foods, cosmetics and the like. Rizorecetin is an amphiphilic molecule, like a general surfactant, and aggregates at a critical micelle concentration (CMC) in aqueous solution to form micelles.

FIG. 1 is a photograph showing the structural difference of liposome particles using general lecithin and lysolecithin.

[Figure 1]

Liposomes as nanostructures have been established with various compositions and ratios of nanoparticles through numerous studies in the past, while rizolecetin has been able to form highly structurally stable nanostructures, but until now only very limited formulations have been used have. Liposomes constituting lipid bilayers are structurally densely packed with two chains of hydrophobic fractions. Therefore, various studies have been conducted to improve stability by using cosurfactants.

However, Rizorecetin has a single structure with a small number of units, so it can be cone shaped to form very dense nanostructure.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a hydrogenated pea extract, which is structurally more stable than the conventional liposome made of lecithin, And extracting sunflower seed oil and rosemary leaf extract into a nanostructure, thereby providing a nanostructure having skin regeneration and anti-inflammatory effect.

The nanostructure according to the first embodiment of the present invention comprises 5.0 to 20.0% by weight of Lysolecithin; 0.5 to 10.0% by weight of hydrolyzed pea (Hydrolyzed Pea) as a hydrolyzed soybean raw material; 0.5 to 10.0% by weight of Boehmia duffusa root extract, a natural product of cosmetic ingredients; 0.01 to 10.0% by weight aqueous solution of a peptide; 0.01 to 0.05% by weight of a sequestering agent; 0.01 to 10.0% by weight of an oil sealant; 0.2 to 2.0% by weight of a moisturizer; And purified water in a ratio of 37.95 to 93.77% by weight.

The oil sealant may be selected from the group consisting of Caprylic / Capric Triglyceride, Squalane, Argania Spinosa Kernel Oil, Tocopheryl Acetate, Macadamia Oil Integrifolia Seed Oil, Helianthus Annuus Seed oil, and Rosmarinus Officinalis Leaf Extract.

The peptide aqueous solution is an epidermal growth factor (EGF), which is selected from the group consisting of SH-Oligopeptide-1, Oligopeptide-1 and Human Oligopeptide-1 ). ≪ / RTI >

The metal ion sequestering agent is characterized in that the disodium EDTA is disodium EDTA.

The moisturizing agent is 1,2-hexanediol.

According to the nanostructure of the present invention, nanostructures prepared using a natural substance composed of a ribofosporide or a lysozyme extracted from a soybean extract are well formed of nanoparticles and are considered to be formed as a bilayer such as liposome. In addition, it can be seen that when the cosmetic ingredient having the skin synthesis or anti-inflammatory effect is contained in the nanostructure, the efficacy is more excellent. This is a cosmetic nanostructure and is effective for skin-beauty as a biocompatible raw material for cosmetics which is excellent in collagen synthesis and anti-inflammatory effect.

Hereinafter, preferred embodiments of the present invention will be described in detail. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

The nanostructure of the present invention comprises 5.0 to 20.0% by weight of Lysolecithin, 0.5 to 10.0% by weight of Hydrolyzed Pea which is a hydrolyzed soybean raw material, Red Hogweed root extract (Boerhavia 0.01 to 10.0% by weight of an oil sealer, 0.2 to 2.0% by weight of a moisturizer, and 37.95 to 93.77% by weight of a purified water, By weight.

According to the present invention, it is preferable that the above-mentioned razor lecithin is a raw material (Glycine soja seed extract) extracted from soybeans, which is refined and contains 90% or more of lyso lecithin.

According to the present invention, the hydrolyzed soybean feedstock is preferably a hydrolyzed pea.

According to the present invention, it is preferable that the natural ingredient of the cosmetic ingredient is Boerhavia duffusa root extract.

According to the present invention, the oil sealant is selected from the group consisting of Caprylic / Capric Triglyceride, Squalane, Argania Spinosa Kernel Oil, Tocopheryl Acetate, It is preferably one selected from Macadamia Integrifolia Seed Oil, Helianthus Annuus Seed oil, and Rosmarinus Officinalis Leaf Extract.

The inventors of the present invention have found that when ni-structured compositions are repeatedly used, it is possible to obtain a nano-structure by adding the natural ingredients and the oil-tight sealant of the raw material of soybean lysozyme, hydrolyzed soybean and cosmetic ingredients, The skin is excellent in regeneration and antioxidant effect, and the present invention has been completed on the basis thereof.

It is preferable that the above-mentioned razor lecithin is selected from raw materials having high purity extracted from soybean. It is preferable that the amount of the Rize lecithin is 5 to 20.0% by weight. If the content is less than 5.0% by weight on the total formulation basis, the specific surface area of the particle surface is increased due to the nature of the nanostructure. Therefore, if the content of the nanostructure is less than 20.0% by weight, This is because excessive force is applied to the physical stirrer, which hinders formation of the nanostructure.

The extract of the cosmetic ingredient is Red Hogweed root extract and its content is preferably 0.5 to 10.0% by weight. This is because when the content is less than 0.5% on the total formulation basis, the skin inflammation effect is reduced as a result of the experiment on In-Vitro, and when the content is more than 10.0% by weight, no statistical difference can be seen in the efficacy and the effect portion.

The oil sealant is preferably 0.01 to 10.0% by weight. If the content of the oil-containing nanostructure is less than 0.01%, the particle size of the nanostructure containing the oil is not known. If the content is more than 10.0% by weight, the particle size of the nanostructure becomes large and the stability becomes poor.

The peptide and the sequestering agent and the moisturizing agent may be selected depending on the degree of effect required by selecting each of the conventional components known in the art to which the present invention belongs, so that the kind and content thereof are not particularly limited.

The peptide may be used as an epidermal growth factor (EGF), such as SH-Oligopeptide-1, Oligopeptide-1 and Human Oligopeptide-1 , And human oligopeptide-1 is more preferable. However, it is possible to select each of the conventional components known in the art to which the present invention belongs, and to use them depending on the degree of effect required, and therefore the kind and content thereof are not particularly limited.

As the metal ion sequestering agent, disodium EDTA and 1,2-hexanediol are preferably selected as moisturizing agents. Disodium diidietite may be contained in an amount of 0.01 to 0.05% by weight, and 1,2-hexanediol may be contained in an amount of 0.2 to 2.0% by weight.

The nanostructure composition according to the present invention may be formulated into cosmetic formulations of various formulations according to the content ratio of the components as described above. Preferably, the nanostructure composition may be formulated in the form of a skin, essence, lotion, Lt; / RTI >

The hydrolyzed soybean raw material is preferably 0.001 to 0.1% by weight based on the composition of cosmetic formulations. This is because, when the content is 0.1% by weight or more, the in vitro test results show that the cell regeneration effect of the skin phospholipid is lowered. When the content is less than 0.001%, the effect and the effect are statistically different I can not see it.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are intended to further illustrate the present invention, but the scope of the present invention is not limited by the following examples.

[Example 1]

In the following Table 1, a nanostructure (hereinafter referred to as a 'peptamycin') composition was prepared according to the following production method using a lyso lecithin.

number Classification ingredient Lot. No. A1 Lot. No. A2 One * Soybean extract Soybean extract
(Glycinesoja (soybean) seedextract) 10.0 10.0 2 Beauty ingredient extract * Red Hogweed root extract
(Boerhaviaduffusarootextract) 0 4.0 3 * Hydrolyzed peas
(HydrolyzedPea) 0 4.0 4 Yeast Extract 0 4.0 5 Peptides Human oligopeptide-1 (10 ppm)
(Human Oligopeptide-1) 0 1.0 6 SOO-oligopeptide-1 (100 ppm)
(sh-Oligopeptide-1) 0 1.0 7 Oil
Sealant Caprylic / Capric Triglyceride (Caprylic / Capric Triglyceride) 0 4.5 8 Squalane 0 0.2 9 Argan Oil 0 0.1 10 Tocopheryl acetate
(TocopherylAcetate) 0 0.1 11 Metal ion sequestrant Disodium EDTA < RTI ID = 0.0 > 0 0.1 12 Moisturizer 1,2-Hexanediol 0 2.0 13 Purified water Purified water 0 69.1 * Soybean extract: (Product name: Lysofix, manufacturer: KEMIN (USA))
* Red Hogweed root extract: (Product name: Mediacalm, Silab (France))
* Hydrolyzed peas: (Product name: Stem-C-Guard, Manufacturer: Silab (France))

[Example 2] Production of cosmetic formulations

The composition of the cosmetic formulation containing the nano-structured 'peptadosomes' as shown in Table 2 of the examples was prepared, and Table 3 of the examples prepared a composition of cosmetic formulations as a comparative example made of the 'peptadose' . The reasons for adding the fruit acid among the raw materials included in the formulations shown in Tables 2 to 3 below were added in order to test whether or not skin irritation was observed in Experimental Example 4 below.

ingredient Example 2 (parts by weight) T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 90.03 87.13 87.47 76.83 65.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 * Peptamus 2.0 2.0 2.0 2.0 2.0

ingredient Comparative Example 2 (parts by weight) S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Disodium edetate 0.05 0.05 0.05 0.05 0.05 glycerin 2 2 3 3 5 Butylene glycol 2 3.5 3 3 7 PIGGY-60 Hydrogenated Castor Oil 0.2 0 0 0 0 Carbomer 0 0.05 0.08 0.2 0.3 Purified water 92.03 89.13 89.47 78.83 67.63 Stearic acid 0 0.1 0 1.2 1.5 Cetearyl alcohol 0 0.05 0 1.5 2 Squalane 0 0.1 0.1 3 5 Sheer butter 0 0.05 0 1.5 2 Beads wax 0 0 0 0.5 1.5 Glyceryl stearate 0 0.5 0.5 1.5 1.5 FAGE -100 stearate 0 0.5 0 One One Sorbitan sesquioleate 0 0.2 0 0.8 1.5 Triethanolamine 0 0.05 0.08 0.2 0.3 Marchia extract 0.5 0.5 0.5 0.5 0.5 Location of Hazel extract 0.5 0.5 0.5 0.5 0.5 Hyaluronic acid 0.5 0.5 0.5 0.5 0.5 Fruit acid 0.2 0.2 0.2 0.2 0.2 1,2-hexanediol 2 2 2 2 2 Spices 0.02 0.02 0.02 0.02 0.02 * Peptamus - - - - -

[Experimental Example 1]: Analysis of particle characteristics

In order to analyze and evaluate the morphology of the particles according to Example 1, particle size analysis using light scattering measurement equipment and evaluation using TEM to check particle shape were carried out as follows.

NANO-ZS90 (manufacturer: Malvern Korea) is a light scattering measurement device used for characterizing nanoparticles and colloids as a particle and molecular size, zeta potential and molecular weight measurement product. The experimental sample used for each light scattering measurement was Lot.No. A1 and Lot. No. As A2, the state of particle size was confirmed by using Lysolecithin contained in soybean extract.

Lot. No. A1. The nanoparticle analysis results prepared according to

Lot. No. Particle analysis according to change with time (1 day) after manufacture according to A2

Lot. No. Particle analysis according to change with time (60 days) after manufacture according to A2

Tables 4 to 6 show experimental data using light scattering measurement equipment. Lot according to Example 1. No. In the case of A1, the average particle size of soybean extract alone was 33.99 nm and the size of nanoparticles was very small. And Lot. No. In the case of the nanoparticles prepared according to A2, the average particle size according to the aging change was 85.58 nm on average after 1 day of manufacture, and the average particle size after 60 days was 130 nm. This indicates that Lysolecithin contained in soybean extract is very stable and excellent as a nanoparticle to be applied to cosmetics.

The prescription Lot according to Example 1. No. The nanostructures prepared according to A2 were analyzed for morphology of particles using a transmission electron microscope (TEM) (Table 7).

Through a transmission electron microscope (TEM), Lot. No. Analysis of particle morphology of nanostructures prepared according to A2

The images in Table 7 are obtained by analyzing the morphology of the nanostructures using a transmission electron microscope. The photographs are almost round in shape, and the average particle size is uniformly distributed below 200 nm. These results suggest that Rizolecitin in the soybean extract of KMMN can form nano-spheres such as liposomes.

[Experimental Example 2]

In order to evaluate the ability of collagen synthesis according to Example 1, this experiment was carried out to confirm the expression level of Procollagen C-endopeptidase enhancer: PCOLCE, which is a marker involved in collagen synthesis in human fibroblast (CCD986sk), at the mRNA level. Specifically, Human fibroblasts were cultured in 100 mM / 60.1 cm ² culturedish at 37 ° C, 5% (w / v) with Dulbecco's Modified Eagle's Medium (DMEM), 10% Fetal bovine serum (FBS) and 1% Antibiotic- Antimycotic (GIBCO, Cat No.15240-062) % CO ² . When human cells were confluent at 100% or more, they were cultured in 96-well plates at 1 × 10 ⁴ cells / well for 24 hours. Then, after the medium was removed, the test sample according to Example 1 was treated with a cytotoxic concentration of 0.001%, after replacing with DMEM free medium (medium lacking FBS). The cells were then cultured for 24 hours under the condition of cell culture to determine the effect of PCOLCE on the expression of PCOLCE. The real-time PCR method was used for the test, and the results are shown in [Fig. 2].

[Figure 2]

The sample prepared according to Example 1 in CCD986 human fibroblast host Lot. No. A1 and Lot. No. A2, which is involved in the synthesis of procollagen. PCOLCE is a gene that directly affects the collagen synthesis process, and each sample has an effect on the expression of PCOLCE to increase the collagen formation. As a result, Lot compared to the negative control group. No. The A1 sample was about 17%, Lot. No. A2 sample increased PCOLCE expression by about 39% compared to the control group. As a result, it has been found that the Rye lecithin, a soybean extract of Boemansa, not only affects collagen synthesis but also increases collagen synthesis ability when forming nanostructures together with cosmetic components, peptides, oils and the like.

 [Experimental Example 3]

In order to evaluate the inflammation inhibitory effect according to Example 1, the expression of COX-2 marker, which is closely related to inflammation, was examined. An enzyme called Cyclooxygenase (COX) exists as two subtypes of COX-1 and COX-2 in our body elimination. Among them, COX-1 is a protective enzyme, found in many parts of the body, whereas COX-2 is induced mainly in inflammation-inducing environments, and lipopolysaccharides, cytokines, growth factors Expression is increased by tumor factor. In other words, cox-2 is an enzyme that promotes the synthesis of inflammatory prostaglandin. In this experiment, human keratinocyte (HaCaT) was cultured in Dulbecco's Modified Eagle's Medium (DMEM), human corneal cells (HaCaT) Were cultured in 100 mm / 60.1 cm ² culturedish at 37 ° C and 5% CO ² with 10% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (GIBCO, Cat No. 152-062). When the human keratinocytes were confluent at 70% or more, they were cultured in 96 well plates at 1 × 10 ⁴ cells / well for 24 hours. After the medium was removed, the medium was replaced with DMEM-free medium (medium lacking FBS), and then irradiated with UV at ⁵ mj / cm ² to induce inflammation. No. A1, Lot. No. A2 was treated with 0.001%, a cytotoxic concentration. The cells were cultured for 24 hours in a cell culture condition to determine the effect of COX-2 on the expression of COX-2. The real-time PCR method was used for the test, and the results are shown in [Figure 3].

[Figure 3]

The sample prepared according to Example 1 in human keratinocyte cell line HaCaT, Lot. No. A1 and Lot. No. A2 is an enzyme that promotes the synthesis of inflammatory prostaglandin. In this experiment, COX-2 reduces the expression of COX-2, which is increased by UV, I looked forward to it. The mRNA level of COX-2 was measured by real-time PCR to confirm COX-2 expression level. The positive control group (no sample added / 5 times higher than the negative control bacteria (no sample added / UV irradiation group), the amount of each sample was expressed as Lot. No. A2, COX-2 expression was reduced by about 2.5-fold. However, Lot. No. A1 did not reduce the expression of COX-2 by UV irradiation. It is judged that the components such as red hogweed root extract and hydrolyzed pea extract, which are cosmetic ingredients according to Table 1 of Example 1, have an anti-inflammatory effect in the state of nanoparticles.

[Experimental Example 4]

The following sensory evaluation was carried out in order to evaluate skin moisturizing feeling, feeling of use, feeling of firmness, improvement of skin condition, feeling after use, and skin irritation effect according to the cosmetic formulation according to Example 2.

First, 30 women who were between 21 and 40 years old were divided into three groups of 10 people. After washing the face of the subject to be tested cleanly, 0.1 g to 0.2 g of the content of Example 2 was uniformly applied to the face half and 0.1 g to 0.2 g of the contents of Comparative Example 2 was uniformly applied to the other half face Respectively. For each formulation, each 7-day trial was discontinued for one week, followed by 7 days. After each 7 days, sensation of skin moisturization, sensation of feeling, feeling of firmness, improvement of skin condition, feeling after use and skin irritation were evaluated, and the results are shown in Tables 8 and 9 below.

When the effect felt at this time was excellent, it was evaluated as 5 points, and when the effect was not felt at all, it was evaluated as 6 points.

Evaluation items Example 2 T1 (skin) T2 (lotion) T3 (Essence) T4 (cream) T5 (pack) Moisturizing 3.50 3.30 3.50 4.00 4.00 Feeling 3.40 3.60 4.20 4.20 4.30 A sense of urgency 4.20 4.40 4.30 4.20 4.10 Improved skin condition 3.30 3.30 3.50 3.40 3.30 After use feeling 4.20 4.10 3.90 4.40 4.10 Skin irritation 0.00 0.00 0.00 0.00 0.00

Evaluation items Comparative Example 2 S1 (skin) S2 (lotion) S3 (essence) S4 (cream) S5 (pack) Moisturizing 0.7 1.2 1.5 1.3 1.1 Feeling 2.8 2.7 3.3 3.1 2.9 A sense of urgency 1.8 1.1 1.4 1.6 2.2 Improved skin condition 1.1 0.8 1.7 1.3 1.1 After use feeling 2.4 2.2 2.6 1.3 1.1 Skin irritation 2.7 2.8 3.3 2.8 2.9

As shown in Tables 8 and 9, the skin was not irritated at 7 days after use according to the formulation of Example 2, and was excellent in moisturizing feeling, feeling of use, elasticity, improvement of skin condition, and feeling after use. On the other hand, in Comparative Example 2, since the nano-structured 'peptadosomes' are not contained, the effect of skin moisturization, feeling of use, feeling of firmness, improvement of skin condition and feeling after use are decreased and skin irritation is also exhibited.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. And will be included in the described technical idea.

Claims (5)

5.0-20.0 wt% of Lysolecithin;
0.5 to 10.0% by weight of hydrolyzed pea (Hydrolyzed Pea) as a hydrolyzed soybean raw material;
0.5 to 10.0% by weight of Boehmia duffusa root extract, a natural product of cosmetic ingredients;
0.01 to 10.0% by weight aqueous solution of a peptide;
0.01 to 0.05% by weight of a sequestering agent;
0.01 to 10.0% by weight of an oil sealant;
0.2 to 2.0% by weight of a moisturizer;
And purified water at a ratio of 37.95 to 93.77% by weight based on the total weight of the nanostructure. The method according to claim 1,
The oil sealant may be selected from the group consisting of Caprylic / Capric Triglyceride, Squalane, Argania Spinosa Kernel Oil, Tocopheryl Acetate, Macadamia Oil Integrifolia Seed Oil, Helianthus Annuus Seed oil, and Rosmarinus Officinalis Leaf Extract. The method according to claim 1,
The peptide aqueous solution is an epidermal growth factor (EGF), which is selected from the group consisting of SH-Oligopeptide-1, Oligopeptide-1 and Human Oligopeptide-1 ). ≪ RTI ID = 0.0 > 21. < / RTI > The method according to claim 1,
Wherein the metal ion sequestering agent is disodium EDTA. The method according to claim 1,
Wherein the humectant is 1,2-hexanedio. 2. The nanostructure of claim 1, wherein the humectant is 1,2-hexanediol.