KR101780111B1 - The composition comprising extracts from fruits of Foeniculum vulgare for enhancing function of skin barrier - Google Patents
The composition comprising extracts from fruits of Foeniculum vulgare for enhancing function of skin barrier Download PDFInfo
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- KR101780111B1 KR101780111B1 KR1020150116132A KR20150116132A KR101780111B1 KR 101780111 B1 KR101780111 B1 KR 101780111B1 KR 1020150116132 A KR1020150116132 A KR 1020150116132A KR 20150116132 A KR20150116132 A KR 20150116132A KR 101780111 B1 KR101780111 B1 KR 101780111B1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
Abstract
The present invention relates to a cosmetic composition for enhancing skin barrier function comprising an extract of fennel or a fraction thereof as an active ingredient. The fructus extract or its fraction according to the present invention increases the expression of the skin barrier constituting peptide, involucrin or filaggrin, in the keratinocyte, and the expression of the hyaluronic acid synthase HAS-1 , And thus the production of hyaluronic acid which is a skin moisturizing factor is increased by enhancing the skin barrier function and the skin moisturizing function of the fennel fruit extract or its fractions as well as the atopic dermatitis, Improvement or treatment of chronic skin diseases such as dermatitis and the like.
Description
The present invention relates to a cosmetic composition for enhancing a skin barrier function and a method for screening a skin barrier function enhancer comprising the fennel fruit extract or a fraction thereof as an active ingredient.
Fennel (Foeniculum vulgare , fennel) is a plant originating in the Mediterranean coast of 1.5-2 m in height. Its stem is straight and hollow. Leaves are long, sheath-like, sharp-pointed, and have 3 to 4 split like feathers. At the end of the branch, a small yellow flower blooms in the form of an umbrella-shaped inflorescence, and the fennel fruit is a divisional fruit and has a sweet taste and aroma.
Fennel contains trans-anethole, fenchone, methylchavicol, estragole, and limonene as its main ingredients. It has anti-inflammatory, analgesic and anti-inflammatory properties. Has been attracting attention as a natural material with superior functionality. Especially recently, it is highly effective in the treatment of various female diseases, and it is known that drinking tea reduces the symptoms of menopausal women and increases the amount of maternal milk. It also stimulates appetite and digestion, and relieves stress and helps sleep. Diuretic action is also used to prevent weight loss and obesity, so it is also called a diet hub.
On the other hand, the skin system, which is an organ covering the outside of the body, is composed of three layers from the outside to the epidermis, the dermis, and the subcutaneous fat layer. In addition to functioning as a barrier against harmful external stimuli, the skin prevents external leakage of moisture and electrolytes in the body, performs sensory functions such as body temperature control, tactile sensation, pressure, and temperature stimulation, D synthesis, and expressing abnormalities of the internal organs.
The epidermis, which is the outermost layer of the skin, occupies most of the keratinocytes of the middle layer squamous epithelium. The dermis layer below the epidermis consists of matrix proteins such as collagen fibers and elastic fibers, and blood vessels, nerves, and glands are distributed.
The upper layer of the epidermis consists of stratum corneum, which is a stratum corneum, which is a layer of bricks. The lipid bilayer composed of phospholipid, ceramide, and cholesterol plays a role in supporting the skin barrier structure. Skin barrier is the most important primary line of defense against toxic substances, microorganisms, mechanical stimuli, and ultraviolet rays, and provides an environment in which the skin can function normally by suppressing electrolyte and water loss through the skin.
Therefore, when the skin barrier is damaged, the skin becomes dry and rough and is easily exposed to the harmful environment, so aggressive chemicals and microorganisms easily penetrate into the skin, which can cause problematic skin such as atopic dermatitis, contact dermatitis, psoriasis, have. As the awareness of the importance of skin barrier has increased, skin barrier enhancement has attracted attention as a new concept of cosmetic agent and skin care, and therapies for improving skin barrier function are being studied to treat skin diseases.
Thus, the present inventors verified the effect of fennel on skin barrier protection and function enhancement. And to develop products with skin barrier function enhancing effect.
The inventors of the present invention conducted studies to confirm the effect of enhancing the skin barrier function of the fennel fruit extract and its fractions and found that the skin barrier peptide Involucrin when treated with the fennel fruit extract or its fractions at the cellular level, Or flaggrin is increased and production of hyaluronic acid which is a skin moisturizing factor is increased, thereby completing the present invention.
Accordingly, it is an object of the present invention to provide a cosmetic composition for enhancing skin barrier function, which comprises an extract of fennel or a fraction thereof as an active ingredient.
It is another object of the present invention to provide a screening method of a substance for enhancing skin barrier function.
However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
In order to accomplish the object of the present invention, the present invention provides a cosmetic composition for enhancing skin barrier function, comprising fructan fruit extract or a fraction thereof as an active ingredient.
In one embodiment of the invention, the extract or fraction thereof is selected from the group consisting of water, C 1 to C 4 lower alcohols, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, And a mixed solvent of water and an organic solvent.
In another embodiment of the invention, the composition may be one that increases the expression of inollucrin or filaggrin.
In another embodiment of the present invention, the composition may be one that increases the production of hyaluronic acid.
In another embodiment of the present invention, the composition may be one which prevents skin dryness and inflammation of the skin.
In another embodiment of the present invention, the composition may further comprise at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, , A body cream, a body oil, a body essence, a makeup base, a foundation, a hair dye, a shampoo, a rinse, and a body cleanser.
In addition, the present invention provides a method for screening a substance for enhancing skin barrier function, comprising the following steps.
(a) treating the test substance with cells expressing involucrin or filaggrin,
(b) measuring an expression level of the gene in the cell; And
(c) selecting a test substance that enhances the expression of an inbolucurin or pilagreen gene as a substance for enhancing skin barrier function as compared to a control group in which the test substance is not treated.
The present invention also provides a method of enhancing skin barrier function comprising the step of administering the composition to a subject.
The present invention also provides the use of a fennel fruit extract or a fraction thereof for the preparation of a skin barrier function enhancer.
The fructus extract or its fraction according to the present invention increases the expression of the skin barrier constituting peptide, involucrin or filaggrin, in the keratinocyte, and the expression of the hyaluronic acid synthase HAS-1 , And thus the production of hyaluronic acid which is a skin moisturizing factor is increased by enhancing the skin barrier function and the skin moisturizing function of the fennel fruit extract or its fractions as well as the atopic dermatitis, Improvement or treatment of chronic skin diseases such as dermatitis and the like.
FIG. 1 shows the results of XTT assay after 24 hrs of treatment with various concentrations of fennel fruit extract or fraction thereof on HaCaT cells, a human keratinocyte cell line, to evaluate the cytotoxicity of fennel extract or its fractions.
FIG. 2A shows the result of confirming the expression of skin barrier constituting peptide (involucrin, loricrin, filaggrin) mRNA by RT-PCR after 24 hours of treatment with human fruit keratinocyte HaCaT cells treated with fennel fruit extract or its fraction.
FIG. 2b shows the results of confirming the expression of the skin barrier constituting peptide (involucrin, loricrin, filaggrin) protein by Western blotting after 24 hours of treatment with the fennel fruit extract or its fraction on human-derived keratinocyte cell line HaCaT cells.
FIG. 3A shows the result of measuring the amount of hyaluronic acid produced by ELISA after 24 hours of treating the human-derived keratinocyte cell line HaCaT cells with the fennel fruit extract or its fraction.
FIG. 3B shows the expression of hyaluronic acid synthase (HAS-1, HAS-2, HAS-3) mRNA after RT-PCR after 24 hours of treatment with human fruit keratinocyte HaCaT cells and fractions thereof .
FIG. 4 is a graph showing the results of RT-PCR of anti-fungal peptides, human β-defeinsin (HBD-1, HBD-2 and HBD-3) and the expression of cathelicidin (LL-37) mRNA was examined.
The present inventors have found that when keratinocytes are treated with fennel fruit extract and its fractions, the expression of the skin barrier peptide, involucrin or flaggrin, increases and the production of hyaluronic acid, which is a skin moisturizing factor, increases The present invention has been completed.
Accordingly, the present invention provides a cosmetic composition for enhancing skin barrier function comprising an extract of fennel or a fraction thereof as an active ingredient.
As used herein, the term " skin barrier " refers to the stratum corneum composed of keratinocytes as the uppermost layer of the epidermis, which is the outermost layer of the skin. It is the most important primary line of defense against toxic substances, microorganisms, mechanical stimuli and ultraviolet rays, and functions to provide an environment in which the skin can perform normal functions by suppressing electrolytes and moisture loss through the skin.
As used herein, the term " strengthening the skin barrier function " refers to enhancing the barrier function of the stratum corneum at the outermost part of the skin. By increasing the expression of involucrin and / or filaggrin, To strengthen the function of.
The fennel fruit extract of the present invention can be extracted using a conventional solvent known in the art for extracting an extract from a natural product, that is, under ordinary temperature and pressure conditions. For example, in the present invention, the fennel fruit extract is composed of water, an alcohol having 1 to 4 carbon atoms, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, And the like. In addition, the method for extracting the extract from the fennel may be carried out by various methods such as hot water extraction, cold extraction, reflux extraction, and ultrasonic extraction, but is not limited thereto.
The extract thus prepared may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. For example, the filtration can be performed using a filter paper or a vacuum filter, the concentration can be carried out using a vacuum concentrator, and the lyophilization can be carried out, but the present invention is not limited thereto.
Further, the extract extracted with the solvent may be further subjected to fractionation with a solvent selected from the group consisting of butanol, n-hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water, . The fractionation temperature may be 4 캜 to 120 캜, but is not limited thereto.
The cosmetic composition of the present invention may contain ingredients commonly used in cosmetic compositions as well as fennel fruit extract or fractions thereof and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes Adjuvants, and carriers.
In addition, the composition of the present invention may be used in combination with an organic sunscreen agent that has been used in the past so long as it does not deteriorate the skin protecting effect by reacting with fennel fruit extract or fractions, in addition to the fennel fruit extract or its fractions. Examples of the organic UV blocking agent include glyceryl paraben, drometrizol trisiloxane, drometrizol, dipaloyyl triolate, disodium phenyldibenzimidazole tetrasulfonate, diethylhexylbutamidotriazone, diethylamino Hydroxybenzoylhexyl benzoate, di-methoxycinnamate, a mixture of Rawson and dihydroxyacetone, methylene bis-benzotriazolyltetramethylbutylphenol, 4-methylbenzylidene camphor, menthyl anthranylate, benzophenone (Benzophenone-4), benzophenone-8 (dioxyphenylbenzone), butylmethoxydibenzoylmethane, bisethylhexyloxyphenol methoxyphenyltriazine, synoxate, ethyl dihydroxypropylparaben, Ethylhexyldimethylpivalate, ethylhexylmethoxycinnamate, ethylhexylsalicylate, ethylhexyltriazone, isoamyl-p-methoxy cinnamate, polysilicon-15 (dimethicone ethylbenzal A carbonate), terephthalic ylidene dikaem peoseol sulphonic Acid and their salts, tea yieyi - may be used salicylate and at least one member selected from the group consisting of the amino acid (paba).
Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, . Specific formulations of the cosmetic composition of the present invention include skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, moisturizing lotions, nutrition lotions, massage creams, nutritional creams, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow and the like.
According to a preferred embodiment of the present invention, the content of the fennel extract or fraction thereof of the present invention is 0.00001-30% by weight, preferably 0.5-20% by weight, more preferably 1.0-10% by weight %to be. If the content of the fennel extract or its fraction is less than 0.00001% by weight, the effect of absorbing ultraviolet light is greatly reduced. If the content is more than 30% by weight, skin irritation may be caused.
In another aspect of the present invention, the present invention provides a method of treating a test substance comprising: treating a test substance with cells expressing involucrin or filaggrin; Measuring an expression level of the gene in the cell; And selecting a test substance which increases the expression of an inbolukurin or pilagreen gene as a substance for enhancing skin barrier function in comparison with a control group not treated with the test substance do.
The test substance may be selected from the group consisting of a microorganism culture solution or extract, a natural product extract, a nucleic acid, and a peptide, preferably a natural product extract, more preferably a fennel extract, but is not limited thereto .
The cell can be selected and used by a person skilled in the art without limitation of the type if the cell is capable of expressing the gene.
In the examples of the present invention, skin barrier function and skin moisturizing and strengthening effect were confirmed by increasing the expression of skin barrier peptide and increasing the production of skin moisturizing factor when fructus extract or its fractions were treated with keratinocyte cell line.
In one embodiment of the present invention, after evaluating the cytotoxicity of the fructus fruit extract and its fractions in the keratinocyte cell line through the XTT assay (see Example 2), the fructan fruit extract or its After the fraction was treated with keratinocyte cell line, the expression of skin barrier constituting peptides was observed. As a result, it was confirmed that expression of inulucrin or filaggrin was increased (see Example 3).
In another embodiment of the present invention, hyaluronic acid synthase-1 (HAS-1) expression increases the production of hyaluronic acid as a result of treatment with keratinocyte-producing cell lines of fructus fruit extract or its fractions (See Example 4).
Therefore, it has been confirmed that the composition of the present invention can prevent skin dryness and inflammation of skin through skin barrier function and skin moisturization enhancement.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.
[ Example ]
Example 1. Preparation of fennel fruit extract and fractions
To 1.0 kg of dried fennel powder, 900 ml of 80% methanol was added and the mixture was extracted for 3 hours. The extract was filtered, concentrated by rotary vacuum evaporator and lyophilized to obtain 92.5 g of extract (M). The methanol extract (M) extracted from the fennel is obtained by using a polarity difference in n-hexane (H), methylene chloride (MC), ethyl acetate (E) butanol; B) solvent was added, and the mixture was divided into two fractions in each step. 90 g of the methanol extract was suspended in water and then mixed with the same amount of n-hexane. The water layer was separated again into methylene chloride. The water layer was further fractionated with ethyl acetate. Finally, After fractionation with n-butanol, the obtained fraction layers were filtered, concentrated and lyophilized to obtain 47.8 g of fraction H, 1.4 g of MC, 1.6 g of E and 5.5 g of B, respectively. The samples obtained after lyophilization were stored in a deep freezer at -78 ° C and used in the experiments.
Example 2. Evaluation of cytotoxicity of fennel fruit extract
In order to evaluate the cytotoxicity induction of the fennel extract and its fractions prepared by the method of Example 1, the fennel extract or its various fractions were added to HaCaT cells as human keratinocyte at 0, 10, 50, or 100 ㎍ / ㎖, and cell viability was measured by XTT assay.
As a result, as shown in Fig. 1, the cell viability of fructus fruit extract (M) and all fractions was 95% at a concentration of 50 μg / ml. However, when the extract was treated at a concentration of 100 / / ml, the survival rate of the extract was 99%, that of the fraction H was 87%, that of the fraction MC was 28%, that of the fraction E was 77%, and that of the fraction B was 84% The remaining fractions were found to induce cytotoxicity. Therefore, the concentration of 50 ㎍ / ㎖, which does not show cytotoxicity in all treatment groups, was determined and the following experiments were conducted.
Example 3. Skin barrier Fennel fruit for function Determine the effect of extracts
The skin barrier maintains the normal function of the physical barrier by forming keratinocytes by the differentiation promoting factors secreted during the terminal differentiation of keratinocytes. In order to investigate the effect of fennel fruit on skin barrier function, fennel fruit extract and its fractions were treated with keratinocyte cell line and the expression of genes secreted during keratinocyte differentiation promotion was examined.
Specifically, HaCaT cells, a human keratinocyte cell line, were treated with 50 μg / ml of fructus extract or their fractions at a concentration of 50 μg / ml for 24 hours, and then subjected to reverse transcription polymerase chain reaction (RT-PCR) and Western blotting Western blotting revealed the expression of involucrin, loricrin, and filaggrin mRNA and protein. As a positive control, calcium (calcium) involved in enhancing skin barrier function was used as a biological factor involved in promotion of keratinocyte differentiation.
As shown in FIG. 2A, RT-PCR showed that the expression of mRNA was increased in the case of involucrin gene in the case of treatment with fennel extract (M) and fractions (H, MC, E, B) , E, and B, respectively. On the other hand, mRNA expression of loricrin and filaggrin genes was not significantly increased by treatment with fennel extract and fraction.
In addition, as shown in FIG. 2B, the amount of protein expressed in the case of the involucrin gene treated with fennel fruit extract (M) and fractions (H, MC, E, B) And it was confirmed that the fraction B was significantly increased. In the case of filaggrin, the amount of protein expression was also increased in both of the treatments with fructus extract and fraction, and in the case of fraction B, it was significantly increased. On the other hand, loricin did not increase protein expression in all treatment groups.
Example 4. Moisturizing effect Identification of the effect of Fruit extract
In Example 3, the effect of the fennel extract and its fractions on the skin barrier function improvement was examined, so that it was confirmed whether or not it affected skin moisturization.
For this, HaCaT cells, a human keratinocyte cell line, were treated with 50 ㎍ / ㎖ of Fructus fruit extract or their fractions, respectively, for 24 hours. The culture broth was recovered and the cells were incubated with hyaluronic acid hyaluronic acid synthase (HAS), HAS-2 and HAS-3 mRNA were observed by RT-PCR. The hyaluronic acid synthase (HAS)
As a result of measuring the amount of hyaluronic acid production, as shown in FIG. 3A, hyaluronic acid concentration was slightly increased in the case of treatment with fennel fruit extract or its fraction, and it was found that it was significantly increased in the case of treatment with fractions E and B .
As a result of observing the expression of hyaluronic acid synthetase, the expression of mRNA was increased in the case of HAS-1 when treated with fennel fruit extract or its fraction, as compared to the control, as shown in FIG. 3b. Respectively. In the case of HAS-2, the expression of mRNA was significantly increased only in the case of treatment with fraction H, and the expression of mRNA was not significantly increased in all treatment groups of HAS-3.
Example 5. Chemical Skin barrier Fennel fruit for function Determine the effect of extracts
Finally, in order to investigate the effect of fennel fruit on the improvement of chemical barrier function, HaCaT cells, a human keratinocyte cell line, were treated with 50 ㎍ / ㎖ of fennel extract and fraction, respectively, for 24 hours, (HBD-1, HBD-2, HBD-3) and cathelicidin (LL-37) mRNA were detected by PCR.
As a result, as shown in Fig. 4, in the case of HBD-1, the expression amount of mRNA was increased in all cases of treatment with fennel fruit extract and its fractions. On the other hand, the expression of HBD-2 was increased only in the case of treatment with fraction M, while in HBD-3, the expression level of mRNA was increased in all of the treatment groups except fraction H. In addition, the expression level of mRNA in LL-37 was slightly increased in all treatment groups. However, all of the antimicrobial peptides showed no significant increase in mRNA expression by treatment with fructus fruit extract and its fractions.
From the above results, it was confirmed that the extract of fennel and its fractions did not significantly increase the expression of the antimicrobial peptide, and thus did not affect the improvement of the chemical skin barrier.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. There will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.
Claims (7)
Wherein the extract or fraction thereof is selected from the group consisting of water, C 1 to C 4 lower alcohols, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, Characterized in that the composition is extracted with a solvent.
Wherein the composition increases the expression of inulinucrin or filaggrin.
Wherein the composition increases the production of hyaluronic acid.
Wherein the composition prevents skin dryness and irritation to the skin.
The composition may be selected from the group consisting of softening longevity, astringent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, , A makeup base, a foundation, a hair dye, a shampoo, a rinse, and a body cleanser.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190037036A (en) | 2017-09-28 | 2019-04-05 | 주식회사 세바바이오텍 | Cosmetic composition for enhancing function of skin barrier and anti wrinkle comprising agastache rugosa leaf extract |
| KR20190060646A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome and/or extracellular vesicle derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
| TWI729694B (en) * | 2020-02-07 | 2021-06-01 | 弘光科技大學 | Sunscreen essential oil composition |
| KR20210094312A (en) | 2020-01-21 | 2021-07-29 | 김윤 | Cosmetic composition for enhancing skin barrier function and manufacturing method thereof |
| KR20220094724A (en) | 2020-12-29 | 2022-07-06 | 오철현 | Glechoma hederacea var extract with skin barrier protection function and its manufacturing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112107513A (en) * | 2020-09-07 | 2020-12-22 | 中山大学·深圳 | Anti-allergy composition and preparation method and application thereof |
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| JP2003238432A (en) * | 2002-02-15 | 2003-08-27 | Fancl Corp | Hyaluronic acid acuumulation-accelerating agent |
| KR100964433B1 (en) * | 2008-11-07 | 2010-06-16 | (주)아모레퍼시픽 | A Method of Screening Material For Improving Skin Functions |
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Patent Citations (2)
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| JP2003238432A (en) * | 2002-02-15 | 2003-08-27 | Fancl Corp | Hyaluronic acid acuumulation-accelerating agent |
| KR100964433B1 (en) * | 2008-11-07 | 2010-06-16 | (주)아모레퍼시픽 | A Method of Screening Material For Improving Skin Functions |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190037036A (en) | 2017-09-28 | 2019-04-05 | 주식회사 세바바이오텍 | Cosmetic composition for enhancing function of skin barrier and anti wrinkle comprising agastache rugosa leaf extract |
| KR20190060646A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome and/or extracellular vesicle derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
| KR20190060651A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
| KR20210094312A (en) | 2020-01-21 | 2021-07-29 | 김윤 | Cosmetic composition for enhancing skin barrier function and manufacturing method thereof |
| TWI729694B (en) * | 2020-02-07 | 2021-06-01 | 弘光科技大學 | Sunscreen essential oil composition |
| KR20220094724A (en) | 2020-12-29 | 2022-07-06 | 오철현 | Glechoma hederacea var extract with skin barrier protection function and its manufacturing method |
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