KR101768162B1 - Skin whitening complex containing trihydroxyisoflavone and morus alba leaf extracts - Google Patents

Abstract

The present invention relates to a skin whitening composition comprising a topical extract and a trihydroxyisoflavone complex as an active ingredient, which comprises trihydroxyisoflavone and a leaf extract to reduce the cytotoxicity of the leaf extract and provide a whitening composition .

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a complex of trihydroxyisoflavone and mulberry leaf extract having a whitening effect,

The present invention relates to a skin whitening composition containing a topical extract and more specifically to a composition for skin whitening which contains trihydroxyisoflavone and topical extract to reduce the cytotoxicity of the topical extract and has an excellent whitening effect.

Several factors are involved in determining the skin color of a person. Among them, factors such as the activity of the melanocyte making the melanin pigment, the distribution of the blood vessel, the thickness of the skin, and the presence or absence of the pigment in and out of the human body such as carotenoid and bilirubin It is important.

Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results. In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to a whitening effect, a safety problem to the skin, a problem of formulation and stability in combination with cosmetics, and the like.

It is an object of the present invention to provide a skin whitening composition containing a topical extract having excellent skin whitening effect while maintaining skin safety and product stability.

In order to solve the above-mentioned problems, the present invention provides a composition for skin whitening comprising an extract of a topical extract and a trihydroxyisoflavone complex as an active ingredient.

In one embodiment of the present invention, the trihydroxyisoflavone may be at least one selected from the group consisting of 4 ', 7,8-trihydroxyisoflavone, daidzin and daidzein.

In one embodiment of the present invention, the trihydroxyisoflavone may be obtained using a microorganism from a plant containing trihydroxyisoflavone.

In one embodiment of the present invention, the microorganism is selected from the group consisting of aspergillus genus, bacillus genus, penicillium genus, rhizopus genus, rhizomucor genus, romayi process (talaromyces) genus Bifidobacterium (bifidobacterium) genus Mortierella (mortierella) genus, Cryptococcus Rhodococcus (cryptococcus) in one selected from the group consisting of genus and micro tumefaciens (microbacterium) may be equal to or greater than.

In one embodiment of the present invention, the plant may be soybeans.

In one embodiment of the present invention, the composition may contain 0.0001 to 10% by weight of the leaf extract and the trihydroxyisoflavone complex based on the total weight of the composition.

In one embodiment of the present invention, the composition may be to whiten the skin through inhibition of melanin production.

In one embodiment of the present invention, the composition may be to whiten the skin through improved pigmentation.

The composition for skin whitening comprising the topical extract of the present invention and the trihydroxyisoflavone complex as an active ingredient is useful as a skin whitening composition for preventing skin cell death, inhibiting melanin formation, and ultraviolet rays (UV), while maintaining skin safety and product stability. The present invention can be effectively used as a skin whitening composition.

Hereinafter, preferred embodiments of the present invention will be described in detail in order to facilitate the present invention by those skilled in the art.

The present invention provides a skin whitening composition comprising an extract of a topical composition and a trihydroxyisoflavone complex as an active ingredient.

The top leaf which is a raw material of the leaf extract of the present invention, which is a component of the composition according to the present invention, is often called mulberry leaf. The upper leaves are Morus alba L. ) or a leaf of a relative plant. Leaves are ovate or long ovate ovate, 3-5 pieces, length about 10cm, pointed end, and hairs on the back vein. The top leaves are known as medicinal substances that disperse the wind heat, make the lungs soft, cool down the heat of the liver, cool the blood, clear the eyes, and grow the hair long.

The mulberry leaf extract used in the present invention is an extract obtained from the upper leaves of Mulberry leaf.

A method for obtaining the extract from the upper leaves can be obtained by using a method known in the art. Preferably, the upper leaves are pulverized and extracted with hot water and used, but the present invention is not limited thereto.

The trihydroxyisoflavone, which is another component of the composition according to the present invention, is preferably at least one selected from the group consisting of 4 ', 7,8-trihydroxyisoflavone, daidzin and daidzein, But is not limited thereto. 4 ', 7,8-trihydroxy isoflavone can be represented by the following general formula (1).

[Chemical Formula 1]

The trihydroxyisoflavone may be one prepared by a general chemical synthesis method.

In addition, the trihydroxyisoflavone may be obtained by using a microorganism from a plant containing the trihydroxy isoflavone. Any plant containing trihydroxyisoflavone is usable, but preferably soybean can be used.

More specifically, an example of a method for obtaining trihydroxy isoflavone through a reaction using a microorganism will be described.

First, about 1 to 6 times, preferably about 3 times, water or at least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, And a mixed solvent of Thereafter, the mixture is extracted and degreased with stirring at room temperature for 1 to 5 times, and then about 1 to 8 times, preferably about 4 times, of water or the organic solvent is added to the degreased plant, followed by reflux extraction 1 to 5 times, After immersing at 10 to 20 DEG C for 1 to 3 days, the residue and filtrate are separated by filtration and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The dye was removed using ether or the like. The aqueous layer was extracted 1 to 5 times with the organic solvent, and the obtained organic solvent layer was concentrated under reduced pressure The organic solvent extract is obtained. After dissolving it in a small amount of methanol or the like, a large amount of ethyl acetate or the like is added to dry the resulting precipitate to obtain an extract containing the daidzin or daidzein of the present invention.

Then, 4 ', 7,8-trihydroxyisoflavone is prepared by passing the extract containing the daidzin or daidzain through a microbial reaction.

The extract containing the daidzein or daidzein is dissolved in ionized water at 5 to 10 times, preferably about 10 times, and sterilized at 121 占 폚 for 30 minutes. After cooling to 30 占 폚, And incubated at 30 ° C for 2 to 5 days. The precipitate collected by centrifugation at 5,000 to 10,000 rpm is washed three times with distilled water and centrifuged to obtain a reaction solution.

The microorganism uses a microorganism that produces a binding enzyme for exocytosis and a microorganism that produces an enzyme capable of transferring a hydroxyl group. Preferably, the microorganism is selected from the group consisting of aspergillus , bacillus , penicillium , rhizopus , rhizomucor , talaromyces , , it may be at least one selected from Bifidobacterium (bifidobacterium) genus Mortierella (mortierella) genus, Cryptococcus Rhodococcus (cryptococcus) and micro tumefaciens in the group consisting in (microbacterium).

The enzyme produced by the microorganism can produce 4'-, 7,8-trihydroxyisoflavone by removing the sugar moiety from the daidzin to generate daidzein and transferring the hydroxyl group thereto.

After 4 ', 7,8-trihydroxyisoflavone is prepared by using the microorganism producing the enzyme as described above, the reaction solution is concentrated under reduced pressure to remove the solvent, alcohol is added to the residue, and the mixture is stirred 1 to 5 times The precipitated salts were removed by filtration and the filtrate was concentrated under reduced pressure to obtain a crude product. The obtained crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) To obtain a certain amount of 4 ', 7,8-trihydroxy isoflavone.

In one embodiment of the present invention, the composition may contain 0.0001 to 10% by weight of the leaf extract and the trihydroxyisoflavone complex based on the total weight of the composition. When the mulberry leaf extract and the trihydroxyisoflavone complex are contained in an amount of less than 0.0001% by weight based on the total weight of the composition, the whitening effect is not sufficiently exhibited. If the mulberry extract is contained in an amount of more than 10% by weight,

The complex of the topical extract and the trihydroxyisoflavone may contain the topical extract and the trihydroxyisoflavone in a weight ratio of 1:10 to 100: 1. If the amount is outside of the above range, the content of either the leaf extract or the trihydroxyisoflavone may be too small to achieve a synergistic effect.

Among the determinants of skin color, the largest portion is the distribution and the amount of melanin in the skin. Various factors affecting the production of melanin are known, and the increase of melanin production caused by ultraviolet rays and thus pigmentation are very important in the cosmetics field.

The composition according to the present invention achieves the effect of inhibiting melanin production, thereby whitening the skin. This can be confirmed in Experimental Example 2 below. In addition, the composition according to the present invention lowers pigmentation caused by ultraviolet rays, thereby whitening the skin. This can be confirmed in Experimental Example 3 below.

The skin whitening composition according to the present invention may be, for example, a pharmaceutical composition or a cosmetic composition.

The pharmaceutical composition for skin whitening may further contain a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure, and other therapeutically useful substances, And can be formulated into various parenteral dosage forms accordingly. Parenteral dosage forms, and may be, for example, but not limited to lotions, ointments, gels, creams, patches or spray formulations.

The dosage of the active ingredient is within the level of those skilled in the art, and the daily dose of the drug depends on various factors such as the degree of progress of the subject to be administered, the age of onset, age, health condition, As a standard, it is generally possible to administer the composition at a dose of 1 / / kg to 200 mg / kg, preferably 50 / / kg to 50 mg / kg, once or twice a day, Are not intended to limit the scope of the present invention.

The cosmetic composition for whitening skin is not particularly limited and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing But the present invention is not limited thereto, and may be manufactured by any one or more formulations selected from the group consisting of a foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The content of the active ingredient is not particularly limited, but may be in the range of 0.0001 to 10% by weight based on the total weight of the composition. When the active ingredient satisfies the above content, it can exhibit excellent efficacy without side effects.

The cosmetic composition may further contain the components included in the functional ingredient and the general cosmetic composition, in addition to the herbal extract and the trihydroxyisoflavone complex prepared as described above. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The cosmetic composition of the present invention may further contain, in addition to the above-described functional additive, components contained in a general cosmetic composition as required. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

The present invention will be described in more detail through the following examples. However, the examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.

[ Example  One]

1-1. Preparation of trihydroxyisoflavone by microbial reaction

(1) Production of soybean extract

6 ℓ of hexane was added to 2 ㎏ of soybeans, and the mixture was degreased with stirring at room temperature for 3 times. Then, 4 kg of 80% methanol was added to 1 kg of defatted soybeans, refluxed three times, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The filtrate was extracted 5 times with 1 L of ether to remove the pigment. And extracted three times with 500 ml of butanol. The total 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract. The extract was dissolved in a small amount of methanol and then added to a large amount of ethyl acetate. The resultant precipitate was dried to obtain 300 g of a soybean extract.

(2) Preparation of 4 ', 7,8-trihydroxyisoflavone using microorganisms

10 g of the soybean extract obtained in the above (1) was dissolved in 100 ml of ionized water, sterilized at 121 캜 for 30 minutes, cooled to 30 캜, and then pre-cultured Aspergillus niger KCCM 11885 5 to 10%. After incubation at 30 ° C for 7 days, the substrate was cleared by thin layer chromatography and the reaction was terminated when the substrate completely disappeared. The culture solution was centrifuged at 5,000 to 10,000 rpm. The collected precipitate was washed three times with distilled water and centrifuged to obtain a reaction solution. The precipitate was added with ethanol (200 ml) and stirred (3 times) The precipitated salts were removed by filtration. The filtered filtrate was then concentrated under reduced pressure to give a crude product. Then, the obtained crude product was separated by silica gel column chromatography (chloroform: methanol = 8: 1 to 4: 1) to obtain 0.12 g of 4 ', 7,8-trihydroxyisoflavone.

1-2. Production of leaf extracts

2 ㎏ of the top leaves were pulverized, 4 liters of hot water was added, and the mixture was refluxed three times and then immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and spray-dried to obtain 500 g of a leaf extract.

1-3. Preparation of mulberry leaf extract and trihydroxyisoflavone complex

After mixing the topical extract prepared in the above manner and 4 ', 7,8-trihydroxyisoflavone in a ratio of 60: 1, that is, 6 g of the leaf extract and 0.1 g of trihydroxyisoflavone, The extracts were reextracted with 70% ethanol aqueous solution by 10 times of the total weight of the extracts, and the residue and filtrate were separated by centrifugation and filtration through a filter. Thereafter, the mixture was concentrated under reduced pressure to prepare a leaf extract and a 4 ', 7,8-trihydroxy isoflavone complex.

[ Experimental Example 1] Cytotoxicity test

The degree of cytotoxicity in the mouse pigment cells was confirmed with respect to the mulberry leaf extract and trihydroxyisoflavone complex obtained in Example 1 and the mulberry leaf extract obtained in Example 1-2 as a comparative example.

(Dolebecco-modified Eagles media) supplemented with 10% fetal bovine serum, 100 nM 2 (10 ng / ml) -O-tetradecanoyphorbol-13-acetate, and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, the cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate, the samples of the above Example and Comparative Example were treated, , Washed with phosphate buffer solution (pH 7.2), and stained with 0.1% crystal violet (Sigma) and 10% ethanol (Et-OH) for 5 minutes at room temperature. The unstained crystal violet was completely removed and then washed three times with phosphate buffer (pH 7.2). Then, 95% ethanol (Et-OH) was treated at room temperature for 1 hour to extract crystal violet from the stained cells. The extract was measured with a PerkinElmer (Lambda 25 spectrophotometer) at a wavelength of 540 nm, Cell survival rates were compared. The survival rate of the cells was calculated according to the following equation (1).

[Equation 1]

Survival rate = (A / B) ⅹ 100

A: Absorbance

B: Absorbance without sample addition

Test substance Cell survival rate (%) Leaf extract 25.6 Mulberry extract and
4 ', 7,8-trihydroxy isoflavone complex 95.6

As shown in Table 1, it was confirmed that the extracts of the mulberry leaf extract and the 4 ', 7,8-trihydroxyisoflavone complex according to the present invention significantly reduced the toxicity of skin cells compared with the mulberry leaf extract alone.

[ Experimental Example 2] Measurement of inhibitory effect on melanin production using mouse pigment cells

The leaf extract and trihydroxy isoflavone complex obtained in Example 1 and the leaf extract obtained in Example 1-2 and the 4 ', 7,8-trihydroxy isobutane obtained in Example 1-1, About flavon, melanin production inhibitory effect was measured by using mouse pigment cells.

First, the mouse melanoma cells (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) derived from C57BL / 6 mice were inoculated into DMEM (Dulbeccos modified Eagles media) The cells were cultured in a medium supplemented with 100 nM 2-O-tetradecanoyphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, the cells were cultured at a concentration of 10 ⁵ cells / well in a 24-well plate, and 10 ppm of each test substance Hydroquinone, the mulberry leaf extract obtained from the above examples, 4 ', 7,8-trihydroxyisoflavone, and a complex complex thereof were added and cultured. At this time, the hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide. Absorbance was measured at 400 nm, and the inhibition rate of melanin production was calculated according to the following formula (Dooley's method) Respectively.

&Quot; (2) "

Test substance Melanin production inhibition rate (%) Leaf extract 30.3
4 ', 7,8-trihydroxyisoflavone 60.6 Mulberry extract and
4 ', 7,8-trihydroxy isoflavone complex 85.5 Hydroquinone (positive control) 58.9

As shown in Table 2, the extracts of the topical extracts of the present invention and the 4 ', 7,8-trihydroxyisoflavone complex of the present invention were obtained by extracting hydroquinone, mulberry leaf extract and 4', 7,8-trihydroxyisoflavone alone It was confirmed that the inhibitory rate of melanin formation was remarkably excellent. As a result, it was found that the mulberry leaf extract and 4 ', 7,8-trihydroxyisoflavone complex of the present invention are excellent whitening compositions.

[ Experimental Example 3] Whitening effect test on human skin

The following whitening effect experiments were carried out in order to examine the whitening effect of the mulberry leaf extract and the 4 ', 7,8-trihydroxyisoflavone complex prepared in Example 1 on human skin.

First, 12 healthy boys were exposed to a 1.5 cm diameter opaque tape at the upper part of the subject's body, and then they were exposed to ultraviolet rays (UVB) of about 1.5 to 2 times the minimum erythema dose of each subject. To induce blackening of the skin.

Each of the 4 ', 7,8-trihydroxyisoflavone and upper leaf extract complex obtained in Example 1 was dissolved in 1% (solvent: 1,3-butyleneglycol: ethanol = 7: 3 ), 1% each of hydroquinone (positive control) and 1% of vehicle (negative control), and one condition was observed for 10 weeks without applying any one. The color of skin was measured with a colorimeter CR2002 (Minolta Co., Japan) every 1 week.

Then, the difference (ΔL *) between the start time of application of the respective test substances and the completion time of the application of the test substances was calculated according to the following equation (3). On the other hand, the whitening effect is judged by comparison of DELTA L * between the sample application site and the control site. When the DELTA L * value is 2, the whitening of the deposited pigment is noticeable. .

&Quot; (3) "

DELTA L * = L * value at the time of completion of application - L * value at the start of application

Test substance The degree of skin color brightness (DELTA L *) Mulberry extract and
4 ', 7,8-trihydroxy isoflavone complex 1.98 + 0.13 Hydroquinone (positive control) 1.90 + 0.11 Vehicle (negative control) 0.50 ± 0.15

As shown in Table 3, it was confirmed that the mulberry leaf extract and the 4 ', 7,8-trihydroxyisoflavone complex according to the present invention exhibited a better skin color brightness than hydroquinone. This is because the mulberry leaf extract and 4 ', 7,8-trihydroxyisoflavone complex inhibit skin cell death and improve pigmentation caused by ultraviolet light to brighten skin color.

Formulation examples of the composition containing the topical extract of the present invention and the 4 ', 7,8-trihydroxyisoflavone complex are described below, but the composition of the present invention is not limited to this example.

[ Formulation Example 1] Lotion-type formulation

The topical extract of Example 1 and the 4 ', 7,8-trihydroxyisoflavone complex 3.00

L-ascorbic acid-2-phosphate magnesium salt 1.00

Water soluble collagen (1% aqueous solution) 1.00

Sodium citrate 0.10

Citric acid 0.05

Lycopene extract 0.20

1,3-butylene glycol 3.00

Purified water balance

(Unit: wt%)

[ Formulation Example 2]

The topical extract of Example 1 and the 4 ', 7,8-trihydroxyisoflavone complex 1.00

Polyethylene glycol monostearate 2.00

Self emulsifying monostearate glycerin 5.00

Cetyl alcohol 4.00

Squalane 6.00

Tri-2-ethylhexane glyceryl 6.00

Sphingo glycolipid 1.00

1,3-butylene glycol 7.00

Purified water balance

(Unit: wt%)

[ Formulation Example 3] Packed preparation

The topical extract of Example 1 and the 4 ', 7,8-trihydroxyisoflavone complex 5.00

Polyvinyl alcohol 13.00

L-ascorbic acid-2-phosphate magnesium salt 1.00

Lauroylhydroxyproline 1.00

Water soluble collagen (1% aqueous solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water balance

(Unit: wt%)

[ Formulation Example 4] Cosmetic liquid preparations

The topical extract of Example 1 and the 4 ', 7,8-trihydroxyisoflavone complex 2.00

Hydroxyethylene Cellulose (2% aqueous solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark glycerin 4.00

Sodium hyaluronate (1% aqueous solution) 5.00

Purified water balance

(Unit: wt%)

Claims (9)

Wherein the trihydroxyisoflavone contains the leaf extract and the trihydroxyisoflavone complex as an active ingredient, and the trihydroxyisoflavone reduces the toxicity of the leaf extract. The method according to claim 1,
Wherein the trihydroxy isoflavone is 4 ', 7,8-trihydroxy isoflavone. The method according to claim 1,
Wherein the trihydroxyisoflavone is obtained by using a microorganism from a plant containing trihydroxyisoflavone. The method of claim 3,
The microorganism may be selected from the group consisting of aspergillus , bacillus , penicillium , rhizopus , rhizomucor , talaromyces , Wherein the composition is at least one selected from the group consisting of bifidobacterium , mortierella , cryptococcus and microbacterium . The method of claim 3,
Wherein said plant is soybean. The method according to claim 1,
Wherein the composition contains 0.0001 to 10% by weight of the leaf extract and the trihydroxyisoflavone complex in a total weight of the composition. The method according to claim 1,
Wherein the topical extract and the trihydroxyisoflavone complex contain the topical extract and the trihydroxyisoflavone in a weight ratio of 1:10 to 100: 1. The method according to claim 1,
Wherein the composition whitening the skin through inhibition of melanin production. The method according to claim 1,
Wherein the composition whitens the skin through improved pigmentation.