KR101759542B1 - 디클로페낙 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법 - Google Patents

디클로페낙 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법 Download PDF

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KR101759542B1
KR101759542B1 KR1020160021692A KR20160021692A KR101759542B1 KR 101759542 B1 KR101759542 B1 KR 101759542B1 KR 1020160021692 A KR1020160021692 A KR 1020160021692A KR 20160021692 A KR20160021692 A KR 20160021692A KR 101759542 B1 KR101759542 B1 KR 101759542B1
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염승식
이다영
이애경
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Abstract

본 발명은 디클로페낙(Diclofenac) 노출에 대응하는 히드라(Hydra magnipapillata) 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것으로, 구체적으로 DDBJ/EMBL/NCBI 유전자 데이터베이스에 축적되어 있는 히드라의 유전자 정보로부터 17,000여 개의 개체(singleton)를 추출한 후, 이에 대한 oligo-probe를 디자인하여, 이를 탑재한 17K Hydra Express Gene Microarray(HEGEM)을 완성하였고, 6, 24, 및 48시간 동안 디클로페낙(Diclofenac) 노출에 의해 발현량이 2배 이상 증가하거나 감소한 유전자 51종을 발굴함으로써, 상기 유전자를 디클로페낙 노출 여부를 확인할 수 있는 바이오마커로 사용하고, 이는 수생태계에서 디클로페낙의 노출 여부를 확인하는데 유용하게 사용될 수 있다.

Description

디클로페낙 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법{Diclofenac responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same}
본 발명은 디클로페낙(Diclofenac) 노출에 대응하는 히드라(Hydra magnipapillata) 유전자 및 이를 이용하여 수생태계 환경오염을 진단하는 방법에 관한 것이다.
디클로페낙(Diclofenac)은 비스테로이드성 항염증제(nonsteroidal anti-inflammatory drug, NSAID)로서, 관절염, 요통, 통풍 등의 치료를 위한 통증, 염증, 발열을 경감시키기 위해 사용한다. 그러나 디클로페낙은 심혈관계 위험을 증가시키는 것으로 알려져 있고, 특히 노인들에게는 문제를 일으킬 수 있다고 한다. 전국 각 도시의 하수종말처리장 수질을 분석한 결과, 무분별하게 버려지고 있는 불용의약품으로 인한 콜레스테롤 저하제, 소염 및 해열 진통제 등이 검출되었다. 이런 의약품들은 인간뿐 아니라 동물과 농산물 생산, 수산물 양식에도 광범위하게 사용되고 있으며, 사람이나 동물에게 투여된 후 그 일부가 그대로 또는 생체 내에서 대사체로 변환되어 소변이나 대변으로 배설되어 환경 속으로 들어가게 된다. 또한 가정에서 사용하지 않거나 사용하다가 남은 약들은 쓰레기통, 변기에 버려지고 제약회사에서 생산되어 유통기한을 넘긴 의약품은 폐기되어야 하는데 그대로 환경에 버려지는 경우도 있어서 수질 등 환경오염으로 인하여 사람의 건강에 영향을 미치거나, 생태계를 교란할 경우가 있다. 이러한 이유로, 최근 환경에 잔류하는 의약품들이 새로운 오염물질로 거론되고 있으며, 사용과 폐기에 따른 지속적 방출로 인하여 하천 및 토양오염을 가중시킨다는 여러 연구 결과가 발표되고 있다.
히드라(Hydra magnipalillata)는 신경세포를 갖고 있는 최초의 다세포 동물로서, 생물 진화의 연구에도 매우 중요한 위치를 차지하고 있다. 이배엽성 동물로서 번식은 무성 및 유성생식에 의하며, 뛰어난 재생능력을 갖고 있다. 히드라에 대한 선행 연구로는 형태형성과 관련된 신호전달계(Hobmayer 등, 2000, Nature 407: 186-189; Arvizu 등, 2006, Differentiation 74: 305-312; Augustin 등, 2006, Dev. Biol. 296: 62-70; Kaesbauer 등, 2007, Bev. Biol. 303: 376-390), 재생과 관련된 신호 및 신호전달계(Bode, 2003, Dev. Dyn. 226: 225-236; Fujisawa, 2003, Dev. Dyn. 226: 182-189; Holstein 등, 2003, Dev. Dyn. 226: 257-267), 세포분화의 신호전달과 관련된 연구(Thomsen 등, 2004, Mech. Dev. 121: 195-204; Philipp 등, 2005, Gene Expr. Patterns, 5: 397-402) 및 조직의 fate 결정 신호(Bielen 등, 2007, Development, 134: 4187-4197)등이 알려져 있다. 이러한 신호전달계는 진화과정을 통해 모든 동물에 공통적으로 존재하므로, 히드라에서의 신호전달계 이상을 다른 동물에도 외삽할 수 있다. 대부분의 동물에서 초기발생과정에만 발현하는 다양한 유전자들이 히드라에서는 성체에서도 그 발현이 유지된다. 따라서, 히드라를 이용하면 시기적인 제한을 받지 않고 이와 관련된 신호전달계의 연구가 가능하다. 또한, 히드라에서 각 세포 형에 대한 분자 마커들이 알려져 있어, 세포분화에 대한 연구도 가능하다. 유전자 및 단백질 발현의 위치화(localization)을 위한 in situ hybridization(ISH) 및 immunohistochemistry(IHC)법도 정립되어 있다. 현재 히드라의 160,000 클론의 ESTs 정보 및 genome data(Chapman 등, 2010, Nature 464: 592-596)가 DB화되어 있어, 다양한 신호전달계에 속하는 유전자들에 대한 정보 검색이 가능하다.
이에, 본 발명자들은 디클로페낙 노출에 대한 특이 유전자후보의 확보 및 검출을 위해, DDBJ/EMBL/NCBI 유전자 데이터베이스에 축적되어 있는 히드라의 유전자 정보로부터 17,000여 개의 개체(singleton)를 추출한 후, 이에 대한 oligo-probe를 디자인하였으며, 이를 탑재한 17K Hydra Express Gene Microarray(HEGEM)을 완성하여, 디클로페낙 노출에 의해 발현량이 2배 이상 증가하거나 감소한 히드라 유래의 유전자 51종을 발굴함으로써, 이를 통해 상기 유전자를 디클로페낙 노출 여부를 확인할 수 있는 바이오마커로 사용하고, 이를 이용하여 디클로페낙의 노출 여부를 확인할 수 있음을 밝힘으로써 본 발명을 완성하였다.
본 발명의 목적은 디클로페낙(Diclofenac) 노출에 대응하여 발현량이 변화된 히드라(Hydra magnipapillata) 유전자 및 이를 이용한 디클로페낙 노출 여부를 확인하는 방법을 제공하는 것이다.
상기 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 51로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 디클로페낙(Diclofenac) 노출 여부 검출용 마이크로어레이 칩(microarray chip)을 제공한다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 히드라와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법을 제공한다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 히드라(Hydra magnipalillata)와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법을 제공한다.
또한, 본 발명은 상기 마이크로어레이 칩을 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트를 제공한다.
아울러, 본 발명은 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트를 제공한다.
본 발명에서, DDBJ/EMBL/NCBI 유전자 데이터베이스에 축적되어 있는 유전자 정보로부터 히드라(Hydra magnipapillata)의 17,000여 개의 개체(singleton)를 추출한 후, 이에 대한 oligo-probe를 디자인하였으며, 이를 탑재한 17K Hydra Express Gene Microarray(HEGEM)을 완성하여, 디클로페낙(Diclofenac) 노출에 의해 발현량이 2배 이상 증가하거나 감소한 유전자 51종을 발굴하여, 상기 유전자를 디클로페낙 노출 여부를 확인할 수 있는 바이오마커로 사용하고, 이를 이용하여 디클로페낙의 노출 여부를 확인하는 방법으로써 유용하게 사용될 수 있다.
도 1은 디클로페낙에 노출한 히드라의 차등 발현 유전자 프로파일링 Hierachial clustering 결과를 나타낸 도이다. 72시간에서 LC50의 약 1/100에 해당하는 7.0 μg/L(ppb)에 6시간, 24시간, 및 48시간 동안 노출하였다.
이하, 본 발명을 상세히 설명한다.
본 발명은 디클로페낙(Diclofenac) 노출에 대응하여 발현이 변화하는 히드라(Hydra magnipalillata) 유래의 유전자를 발굴하여, 상기 디클로페낙 노출에 대하여 발현량이 변화하는 히드라 유래 유전자를 집적한 마이크로어레이 칩을 디클로페낙 노출 여부 검출 및 수생태계 오염 상태를 진단하는데 이용할 수 있다.
본 발명은 서열번호 1 내지 51로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 디클로페낙 노출 여부 검출용 마이크로어레이 칩(microarray chip)을 제공한다.
상기 유전자는 히드라(Hydra magnipalillata)로부터 유래된 것이 바람직하고, 서열번호 1 내지 40으로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 디클로페낙 6, 24 및 48시간 노출에 의해 정상 대조군에 비해 발현량이 2배 이상 증가하는 것이 바람직하며, 서열번호 41 내지 51로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 디클로페낙 6, 24 및 48시간 노출에 의해 정상 대조군에 비해 발현량이 2배 이상 감소되는 유전자인 것이 바람직하다.
상기 디클로페낙 노출 여부 검출용 마이크로어레이 칩은 당업자에게 알려진 방법으로 제작할 수 있다. 상기 마이크로어레이 칩을 제작하는 방법은 하기와 같다. 상기 탐색된 유전자를 프로브로 이용하여 마이크로어레이 칩의 기판상에 고정화시키기 위해 파이조일렉트릭(piezoelectric) 방식을 이용한 마이크로피펫팅(micropipetting)법 또는 핀(pin) 형태의 스폿터(spotter)를 이용한 방법 등을 사용하는 것이 바람직하나 이에 한정되지 않는다. 상기 마이크로어레이 칩의 기판은 아미노-실란(amino-silane), 폴리-L-라이신(poly-Llysine) 및 알데히드(aldehyde)로 이루어진 군에서 선택되는 하나의 활성기가 코팅된 것이 바람직하나 이에 한정되지 않는다. 또한, 상기 기판은 슬라이드 글라스, 플라스틱, 금속, 실리콘, 나일론 막, 및 니트로셀룰로스 막으로 이루어진 군에서 선택될 수 있으나 이에 한정되지 않는다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 히드라와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법을 제공한다.
상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것이 바람직하고, 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것이 바람직하다.
또한, 본 발명은
1) 피검 시료에 노출된 실험군의 히드라(Hydra magnipalillata)와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
2) 단계 1)의 RNA를, 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법을 제공한다.
상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것이 바람직하다.
또한, 본 발명은 상기 마이크로어레이 칩을 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트(kit)를 제공한다.
상기 키트는 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하고, 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것이 바람직하다.
아울러, 본 발명은 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트를 제공한다.
본 발명의 구체적인 실시예에서, 본 발명자들은 디클로페낙 노출에 대응하는 히드라(Hydra magnipapillata) 유래의 유전자를 발굴하기 위하여, DDBJ/EMBL/NCBI 유전자 데이터베이스에 축적되어 있는 유전자 정보로부터 히드라의 17,000여 개의 개체(singleton)를 추출한 후, 이에 대한 oligo-probe를 디자인하여, 이를 탑재한 17K Hydra Express Gene Microarray(HEGEM)을 완성하였고, 6, 24, 및 48시간 동안 디클로페낙(Diclofenac) 노출에 의해 발현량이 2배 이상 증가하거나 감소한 유전자 51종을 선별하였다(표 2 및 표 3 참조).
따라서, 상기 유전자들을 포함하는 바이오마커를 프로브로 이용하여 피검체와 대조군의 RNA를 추출하여 상기 마커 유전자들의 발현 변화를 비교분석함으로써, 피검체가 디클로페낙에 노출되었는지 여부를 확인할 수 있다. 즉, 만약 피검체가 디클로페낙에 노출되었다면, 표 2에 기재되어 있는 유전자들의 발현량이 대조군에 비해 피검체에서 증가한 것으로 나타날 것이고, 표 3에 기재되어 있는 유전자들의 발현량은 대조군에 비해 피검체에서 감소한 것으로 나타날 것이므로, 이를 이용하여 피검체의 디클로페낙의 노출 여부를 확인할 수 있다.
이하, 본 발명을 실시예 및 실험예에 의해서 상세히 설명한다.
단, 하기 실시예 및 실험예는 본 발명을 예시하기 위한 것일 뿐, 본 발명이 하기 실시예 및 실험예에 의해서 한정되는 것은 아니다.
< 실시예 1> 특이 유전자 후보군의 분리 및 동정
<1-1> 히드라의 배양
히드라(Hydra magnipapillata)의 야생형(wild strain) 105는 1 mM NaCl, 1 mM CaCl2, 0.1 mM KCl, 0.1 mM MgSO4, 1 mM Tris(hydroxymethyl) aminoethane (pH7.6)에 배양하였다. 수온은 20℃로 고정하였으며, 이틀에 한번 갓 부화한 Artemia 유생을 먹이로 공급하였다. 먹이 공급 후, 수 시간 이후에 배양액을 교환하였다.
<1-2> 디클로페낙 노출 조건
히드라에 디클로페낙을 노출하는 조건을 확립하기 위해, 디클로페낙에 대한 반수치사농도(Lethal concentration 50, LC50)를 결정하였다(표 1).
노출 시간(시) LC50 (mg/L)
24 13.02
48 8.15
72 7.05
상기 결과를 바탕으로, 차등발현 유전자 검출을 위한 노출 농도는 72시간의 LC50 값(7.05 mg/L)의 약 1/1,000에 해당하는 7.0 μg/L로 결정하고, 히드라 20개체에 각각 6, 24, 및 48시간 동안 노출하였다.
<1-3> RNA 추출 및 cDNA 합성
각 노출군의 RNA 추출에는 Tri-reagent(Molecular Research Center Inc.)를 이용하였다. 추출된 RNA를 주형으로 역전사효소를 이용하여 cDNA를 합성하였다.
<1-4> Microarray 실험
마이크로어레이(microarray) 실험을 위하여, 형광물질(Cy5 및 Cy3)이 라벨링된 cRNA 시료를 Qiagen PCR purification kit을 사용하여 정제하고, 증류수로 용출하였다. 그런 다음, 정제된 형광표지-cDNA 시료를 hybridization buffer(3×SSC, 0.3% SDS, 50% formamide, 20 ㎍ Cot-1 DNA, 20 ㎍ yeast tRNA)에 첨가한 후, microcon YM-30으로 농축하여 hybridization mixture를 만들었다. Hybridization mixture를 95℃로 3분 동안 가열하여 변성시키고 12,000×g에서 30초간 원심분리하며 온도를 낮추었다.
그 후, 제조된 히드라 마이크로어레이에 커버슬립(coverslip)을 덮고, 변성시킨 hybridization mixture를 파이펫으로 주입한 후, GT-Hyb Chamber에 넣고 65℃에서 16시간 동안 반응시켜, Hybridization을 수행하였다. 다음으로, chamber에서 마이크로어레이를 꺼내어 세척한 후, 마이크로어레이를 spin하여 건조한 후 scanning할 때까지 암소에서 보관하였다.
실험이 완료된 히드라 마이크로어레이를 Axon GenePix 4000B scanner(Axon Instrument, USA)를 사용하여 스캔하였다. GenePix Pro 6.0 프로그램을 이용하여,스캔한 도면으로부터 각 spot을 gridding file을 이용하여 그리딩(gridding)하였고, 이를 정량하여 각 spot의 Cy5/Cy3 세기(intensity) 및 비율 등의 분석 값이 포함된 GPR file을 얻었다.
상기 GenePix Pro Program에서 얻어진 GPR file로부터, 분석 프로그램인 GeneSpring 7.3.1 (Agilent Technologies, USA)를 이용하여 분석을 수행하였다. 정규화(Normalization)는 LOWESS (locally weighted regression scatterplot smoothing)를 이용하여 수행하였고, Reliable gene은 중앙값(median)의 합이 기본(background) 값보다 낮거나, 각 픽셀(pixel) 값의 표준편차가 유의하지 않은 spot을 flag-out함으로써 유의한 유전자를 얻었다. Significant genes는 정규화한 중앙값이 2배 이상 차이를 보이는 spot을 선택하여 선별하였다.
< 실험예 1> 특이유전자후보군의 선별
디클로페낙 노출실험은 독립적으로 3회 실시하였고, 각 실험결과 중 통계적으로 유의하게 발현량이 변화된 유전자를 확인하여, 이들 중 발현량이 2배 이상 변화되는 유전자들을 선별하였다.
그 결과, 도 1의 유전자 프로파일링에 나타난 바와 같이, 6시간 노출군에서 30종의 유전자(증가 25종, 감소 5종); 24시간 노출군에서 20종의 유전자(증가 11종, 감소 9종); 48시간 노출군에서 31종(증가 27종, 감소 4종)의 유전자가 발현이 변화되는 것으로 분석되었다(도 1).
이들 중 중복되는 유전자들을 제외하면, 총 51종(증가 40종, 표 2; 감소 11종, 표 3)의 유전자들이 디클로페낙 노출에 의해 발현량이 변화되는 것으로 파악되었으며, 이들 유전자들은 디클로페낙 노출을 확인할 수 있는 바이오마커로 유용하게 사용할 수 있음을 확인하였다.
디클로페낙 6, 24 및 48시간 노출군에서 발현량이 2배 이상 증가되는 주요 유전자 목록
서열번호 유전자
1 5-azacytidine induced 1, mRNA
2 Adal protein, mRNA
3 ADP-ribosylation factor-like 11, mRNA
4 Alpha-crystallin B chain, mRNA
5 arginyltransferase 1, mRNA
6 ATP-binding cassette, sub-family C (CFTR/MRP), member 2, mRNA
7 ATP-dependent DNA helicase PIF1, mRNA
8 chondroItin 4-sulfotransferase, mRNA
9 crinkled CG7595-PB, mRNA
10 Cysteine-rich protein 1, mRNA
11 erythrocyte membrane protein PFEMP3, mRNA
12 Ferric reductase like transmembrane component family protein, partial mRNA
13 GCN1 general control of amino-acid synthesis 1-like 1, partial mRNA
14 heat shock protein , partial mRNA
15 heat shock protein 90, alpha (cytosolic), class A member 1, mRNA
16 heat shock protein, mRNA
17 heparanase, mRNA
18 HMBA-inducible, mRNA
19 hydra Na channel 3 (hynac3), mRNA
20 Hydroxymethylglutaryl-CoA lyase, mitochondrial, mRNA
21 Late histone H2A.2.2, mRNA
22 lens intrinsic membrane protein 2.1, mRNA
23 Mib1 protein, partial mRNA
24 mitochondrial RNA ligase 2, mRNA
25 Mitogen-activated protein kinase-activated protein kinase 5, partial mRNA
26 mucin 1, cell surface associated, mRNA
27 Niemann-Pick disease, type C2 , mRNA
28 oxidative stress protein, mRNA
29 phosphatidylinositol glycan, class B, partial mRNA
30 Probable voltage-dependent N-type calcium channel subunit alpha-1B (Voltage-gated calcium channel subunit alpha Cav2.2) (DOE-4), partial mRNA
31 protein tyrosine phosphatase, non-receptor type 14, partial mRNA
32 radial spoke head 10 homolog B2, mRNA
33 Sedoheptulokinase , partial mRNA
34 small heat shock protein, partial mRNA
35 Sox10, mRNA
36 testis expressed gene 264, mRNA
37 Tumor necrosis factor, alpha-induced protein 8-like protein, mRNA
38 U2-associated SR140 protein, partial mRNA
39 WntX2, mRNA
40 WW domain-containing oxidoreductase, partial mRNA
디클로페낙 6, 24 및 48 h 노출군에서 발현량이 2배 이상 감소되는 주요 유전자 목록
서열번호 유전자
41 casanova, mRNA
42 histone H2B, mRNA
43 peroxidase ppod11, partial mRNA
44 polycystic kidney disease 2, mRNA
45 Polycystic kidney disease 2-like 1 protein, partial mRNA
46 Sox17 alpha, mRNA
47 SRY-box containing gene 32, mRNA
48 stalk protein, mRNA
49 tetratricopeptide repeat domain 29, partial mRNA
50 thrombospondin type 1 repeat-containing protein 2, mRNA
51 tyrosine kinase receptor, mRNA
본 발명에서 제시한 디클로페낙((Diclofenac) 노출에 대응하는 히드라(Hydra magnipalillata)의 유전자는 수생태계의 내분비계장애물질 오염 및 이에 따른 수생태계의 상황을 모니터링하고 진단하기 위한 바이오센서의 성분으로 유용하게 사용될 수 있다. 아울러, 제시된 유전자들의 기능에 의거하여 본 생물의 대사/생리변화를 구체화함으로써 앞으로 일어날 수도 있는 병리적 현상을 예측할 수 있는 생체지표 및 센서로 이용할 수 있으며, 수생태계의 스트레스원 검출 또는 건강 진단방법에 효과적으로 이용될 수 있다.
<110> Korea Institute of Ocean Science and Technology <120> Diclofenac responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same <130> 2015P-12-049 <160> 51 <170> KoPatentIn 3.0 <210> 1 <211> 1656 <212> RNA <213> Hydra magnipapillata <400> 1 atggtcgtaa aagatgctga ctcgttacgg aaaaagttta atattgcata ttacttggca 60 aaacaagagc gtccattcac agattatcct tatttgattg cattagaaaa gactaatggg 120 gttacaaatt ttggaaattc taatgtcact gaccgtgcag ctgcaatatt tacagattac 180 attggcacca agcacagatt cagctgtatc ggaacaggaa ttgatttaca ttctttattt 240 aaaagacgga agtccgaaag agcttatgct ggtttaggcg cattactaaa ggaaggttct 300 tcttggctgg aagttgtaca ctgttttaac catcgacttg aacttgcttt aaaagatgcg 360 tttgaaagtt tatctgcctt caaaactgtt gacgaactcc ttttacaact ttattacttg 420 tatcaaaagt caccaaagcg ctaccgagaa ctacaaggat tagccaaagc ttggggtaat 480 agcgttccga aaccaacaaa tgcatgcgga actcggtgga ttgatcacaa atataaagca 540 ataaaaattg ctttagaaaa ttatagtaca gatgttatga atgctgatga acgagtggct 600 caaaaatata tgcagcagtt ggatgattta agggcacaat atgaaataga taaggagact 660 gcagttcgaa aagaaaggga aaactgcaga gagcgacttg aacaacaaat tcaagaggaa 720 gaaaactcat ttcaacaaca acgtcggcgc ttgtattctg aaatagaaga agaaaagcag 780 cgagttagta acctgcttca aacagcaaaa aaagaatttg atgagagaag aactgaatat 840 gaaaacacat ccaagcaaat attggcatct ttacaagagg atttaaaaaa gcaacttcat 900 gaaatgcaag aaaactgcca aaatgagatg cgcaattata aagagcaatt agaaattgaa 960 aaacaacaat ggattgaaaa ttttatgaaa aagcaagaga cccaccttct gtcaaaagaa 1020 cgagaattaa aagataaagt aaaggtggcg agagatcaag aaattgagat ggtcattgat 1080 aagttagaaa gagaaactgc acaaagcaga gaagatgctg aacgtgctgc tgaaaacaga 1140 atcaaacgaa ttcgtgataa atacgtaaat gagataaaag attatgagca atcagagcga 1200 gcacttcagg ataaatgtaa tagtttgaaa gaacaaattg aaaaacttga aaatgatctc 1260 attcagttga aaagcagttt gaagcacaaa gatcaagaag taattgatgt caaaaaaatt 1320 actgatcggt tacaagaaga aagaggaaaa gtaagtgaca tcatccgaca ggaatatgca 1380 gacagattgg tggctactga agaagaatct aaccgcttga gaaaagaact aagtgaagag 1440 aaagctcgtc gtcgttttga agtagaaaga ataacaaaag aaaaagaaaa agaaatggat 1500 gaactacatg atcgtgtaaa aaaggctata tcgaagaaag aagaaactgc gaatttgctt 1560 attcagcaaa aaatagctgc agaaaaacga gctgaacatc tcgaacaatt acttacagag 1620 cagcgcaaaa aaattcttgc caagtgaata actttt 1656 <210> 2 <211> 1068 <212> RNA <213> Hydra magnipapillata <400> 2 atgaaattat ctcttgaaga attttgtttt gctataccga aagtagaact tcatgctcat 60 ctcaatggat ctcttagctt taaaacaatc caaaaactaa ttcaaatcca caataagaaa 120 tggcctctgg aaaatatccc ttctgaaacg gaaatattat ctttagctaa aaaagaccta 180 gatattgaag aggtgtttaa gctgttccca ttgattcaaa aattaacaga tcaccctgaa 240 gcaataaaat ttgcaactga aagtgtgata aaggattttg cagatgaaaa tgttagatac 300 ttagagttaa gaagcacacc cgtaaatgtt aatggtaaaa tgactaaaca tgagtatgtg 360 caggctatta tagatggaat aatgtgttgt gaaatgtcta gtcctttaat taaagttaag 420 cttttactgt ctgttaatcg aagtcaatcc ctggtatcag caaaagaaac tgtgttgata 480 tgtcaagact ttcacaagaa atatccaaat atagttgtag ggatagactt atctggtgat 540 cccaaaaaag gtgatgttat accattactg aaagttttta actctttaag aaaagaatca 600 agtcttaagt tttcttttca tattgcagaa atacatggaa tgcataatga gactgaagtt 660 ttgttaagtt ttttcccaga tagagttggt catggtacat ttattcatcc agattctggt 720 gggtcacaac atttatatga cattttagca aagcaaaaca ttcctgttga agtttgctta 780 acctctaatt gtttaacaaa aactgtgaca aattatcagg acagtcatct tgttccactt 840 tataaatctg cacacccaat agtgatatgc actgatgata agggtgtatt taactgtaat 900 ttgactaatg aatatgtaat agttgctaaa tgtttgggtt taagcaaaga agatatttat 960 aaactatcat taaagagtat tgattatata tttagtgcat cgaatgtaaa agaatatcta 1020 aaagcacaat ggaaagattg gctagatgaa cataactacc ttttttaa 1068 <210> 3 <211> 819 <212> RNA <213> Hydra magnipapillata <400> 3 atggaataca aagaaacctg tctaaagctt aaaattgcaa agcaactaaa aagcaactat 60 gggtttatgg ctatcaaagt tagcatcagc tttaaatgat tttggtaata gaagagctcg 120 cattctttta cttggtttag atgcagctgg aaaaacaaca atactttata agttaaagct 180 caacgaaaac gtcacaacca ttcctacaat cgggtttaat gtggaagagg tgactccagt 240 aaaaaacgtt acttttacca tgtgggatgt tggaggtcag gaaaaaattc gacctttatg 300 gagacactat tatcaaggtg ccgaaggact agtttttgtt gttgatgctt gtgacgtttt 360 aagaattcaa gaagcgcgtg aagagttttt ttctgtttta aaagatgaag gcattgaaaa 420 aggaattcct gcagtgattc ttgcaaacaa acaagatctc ccaaatgcat taaaatcgtg 480 ggaactagtt gataaaatgc ggttaaaaga attaagcggt aatccttggc acgttcaaga 540 aatgtgcgct ttaacagggg atggtttata tgaaggtatt caaagacttg cagatatggt 600 aaaaacatat caaagagaaa acaaagttag atggtaacag catgaaacta aagtaaaaaa 660 tgtataatca tcaacaatat tattcctatc acgatgataa taaataaagt actattttat 720 cagtgaatat ttttttaaat ttttagcttg taaactattt aacttacttg aaaatatctt 780 tataaaatcc gaattttttg attaataaca aggaaaaaa 819 <210> 4 <211> 966 <212> RNA <213> Hydra magnipapillata <400> 4 atttaaagtt ttgtgttgtt tagccataaa gcgcaacgta acgactaaaa aagaagtaag 60 aaagctactt aaaaagtgag aaatgtcttt gtggcacatt cctgttcata aatattttga 120 caccgatcca ttcttggatg acataatgga gataactttt cctcctttaa gatattttcc 180 attatttaat gttggaacaa atgctgtttc aaaaaaaaag actccgaaag aaaatggttt 240 tgttgtaaat cttgatgtta agcattacaa gccagaggaa gttactctta aagtagaagg 300 tcaagttctt gaagtcagtg gaaagcatcg taacgaaaat gaaaatggat ttgagtctag 360 tgagttccac agaaagtaca ctattcccga tgacgtggat gcgacagccc ttacgtcaaa 420 catcagtcaa ggcggcgttt tgcatataga agctcctaaa aagctccctg catcttctgg 480 agaatcaacg aaagagactt ttaaattctc tttagatgta caaggcttca agccagaaga 540 aatttcgatt caagtaaaag gtagagattt agtcgttcat ggtgaaacaa aaactgaaaa 600 cagcggtgaa cacggttcaa gttaccacca caagcagttt accaaacacg tagctttgcc 660 agatgatgta gatccatccg aattatgttc tcgctatacg aaagactcta aattaacgat 720 cgaagctccg cgaaagcaac tgcaagctcc gcttaaactt gaaatcaaaa tggaagaata 780 aagtggtgtc ttttacaatt gtttgtagtt gtgtttgtag ctatttgtaa attttatttt 840 attacaacta aatagtaatg tttacgctaa atgtactata ttttaaatgg cttattttgt 900 tttaaattat aaatttttgt attgatagtt tatttaaaaa agaaaaagac ttatttatta 960 aacaaa 966 <210> 5 <211> 1450 <212> RNA <213> Hydra magnipapillata <400> 5 tttttttatt aggttcatgc gcaaagttat gaataaaagt ttagttgaat attttggaag 60 cgaccaaaca ggacattctt gtggatattg caagaagtta aatggaagtt tgagtcatgg 120 tatgtgggct cattatctgc agtgtcaagg ttacatggag ttaatagaca gagggtggcg 180 aagaagcgga aagtattgtt ataaacctat catgaacaaa acttgctgtc cacaatatag 240 tatcagatgc gatgcaacaa atatcaagtt gacaaaatcc caaaaaaaag ttgttaaacg 300 ttttcataag tttctcacca atggtttaaa aaatgaaaag caaagcttgg ttaacaatat 360 ggaaaatttt gatgatgaat tacatgaagt tcataaagtc gaaaagttaa attttgattt 420 ttgtgaaaac tctaatgcat ttcaaagtac tattaaaaat tgtcctatgt tagaaactgt 480 atcttctcaa atgacttttt atgtaacttc tgaaaatact aataaagatc gtcctttgtt 540 agaaactgta tcttctcaaa tgacttctca agcttctcaa atcaaaccag ttaaagattg 600 tttgactaat cttaaagcta aaaatatcag aaaagaacga aaaattacaa aattaaaaaa 660 aaaagctgaa gagaatggat tattatttga tgaatctagt tttcaaaaaa aacaaaagtg 720 tcccgaaaaa tcgattgaag acttattaga aggtttgcca aataatggag ttcataaact 780 tgagttaaag cttgttcaat gttgccctca atctgaagag taccagcgca cctttcataa 840 agaacatcta ttgtttatta agtatcaaat gcttattcat aaagaatcta ttgaagattg 900 ttctgaagca aagtttcttc gttttcttgt taaaggtcca cttgagccag aaagcattta 960 tggtgcttat catcaacaat attatttaga tggtaatcta attgcagtgg gtgtattgga 1020 tatacttcca ttagctgttt catcagttta ttttttttat gatcctgact atagttttct 1080 ttcacttggc acttattctg ctataagaga aatttatctg gtccgtgaat tacagaagca 1140 gcaccatgat cttaaatatt attacatggg tttctatatt cattcctgtt cgaaaatgag 1200 atacaagaaa caatattatc catctttttt gttatgtcca gaaacttact catggatatc 1260 aatagaaaaa gcactaccaa agttggatat tagtaaatat agtcgtctta gtgatggcat 1320 catcaaaggt gtgacatatc ttcaacgaat tacagaccgc catgaaaaag aaaaacaaat 1380 aaaggaagtt gaagaatatt taactttgac tggtttatca gctttaaata tgattcttta 1440 cagaagttaa 1450 <210> 6 <211> 1962 <212> RNA <213> Hydra magnipapillata <400> 6 atgccattat taacttacaa aaattgtgca tatagagtga gaaaaaccag gtatgataaa 60 acacctaaat gttgtgaaac tactgaaggt gtaaatattg gaattattaa caatatggtt 120 gaaccgcaac aagctttagg ctttaccaat cttgcatata catcaaatag ctgtgtatca 180 gtttttgaag atttaaaaaa taatgagtct gacaaaaatg atactattga tgaaaatgag 240 ttgtacaaaa aagaggccca gctagttctt gcacgccatg aaaaaaccat ttcgttgaaa 300 ggttcagaag ttttaatttc ccaagaagtt tctaaaactg gaaaggtgaa gcgttctgtt 360 tatttgactt actttaagtc aatgagcatt ttgttgacta tcttgttcct attttttggt 420 ttgatatcag aaggtttttc actctgttca agtatttggt tagctgaatg gagctctaac 480 caaaacgcct ctaatcatga acaagattta tatcttggta tttatggagc actaggcgtt 540 tcacaaggtt tatcagcaat gtttcgagca gtatttcttt gttttggtgt cataaaagca 600 tctaaagcct tgcataataa tctgctaaag aatgttctaa gatgtcctat gtcatttttt 660 gaaagcaccc cagctggacg gattgtcaat agattttcaa aagacattaa tttaattgat 720 gagtttattc caatgaccat aaaatcattt gtttcatgtt tttatacatt atgtggaaca 780 atatttataa taagctatac tactcctatt tttcttgctg catttgtacc tattggtgta 840 gcctacattc taactcagcg cttttatgtg gtatcatcac gacaacttca acgtattgaa 900 tctgtaaggc gatcaccaat ctacaatcac ttttttgaaa gtataaatgg tgcatctact 960 atacgcgctt atcgtttaaa tgatgagttt atatctgaaa atgaatctaa aattgatttt 1020 aatcaagaag ccagttttcc tataatatgt tcaaacagat ggttagcact gcgtttggaa 1080 acctttggtc atctcataac tttctttgca gctttgtttt gcattattca acaagaaagt 1140 ttatcacctg gattagttgg tttatcaata tcttatgcac tccaggtaac acaaacatta 1200 aattggttgg tacgagtgtc aagtgaactt gaaactaaca ttgtttctgt agaaagagta 1260 aaagaataca ttgatcttcc tagtgaggga actcaagtta ttcttaatag tcagcttcag 1320 aatgattggc caaatgctgg ggccattgtg tttcaaaacc tttgtttaag atatcgtaag 1380 gatttggctc tggttttaaa gaatataaca tttaatgtga aaccttcctc aaaagttggt 1440 ttagttggtc gaagtggtgc tggtaagtct tcaatagcaa atgctcttta ccgaatttta 1500 gaaccttcat caggttctat tctaattgac aatgttgata tttcaaccat tggtttgcat 1560 gatctaagat ctcgaataac tattatacca caagatccag tactattttc tggaacactg 1620 cgttttaata ttgatccatt taatcaattt gatgatgctg aaatttggag ggttttagaa 1680 attttaaatt taaaattgca tgtttcaaat ttggagggtg gtctgttaca tgagattctt 1740 gaaggaggaa aaaacataag tgttggtcat agtcagctgg tgtgtgtggc tcgtgctttg 1800 cttaaaaaaa gtaagattct tgttcttgat gaagctacat cttcaataga tcttgagaca 1860 gatgcattta ttcaagaagt tattcgaaaa gagttcaaat ctagtacagt gttgtgcatt 1920 gctcatcgat taaacacaat tctggattat gacaaaatat aa 1962 <210> 7 <211> 2736 <212> RNA <213> Hydra magnipapillata <400> 7 atgactttat ctgctaaaca aaaattggct tttcaatggt ttgacgaagg attaaatttt 60 tttattactg gtagtgctgg ttgtagtaaa agttatatac ttaatgctat tgcttctagc 120 gaacaaatgt ttaaaaatat tgcagttaca gctagtacag gaaaagctgc tcatcagatt 180 aaaggtatga ctgtgcacag ttttgcaggt attgaaatag gtacaaaatc tgtcgattat 240 tattacaaac acatgcaaat agatgtttta gaaagatgga gaaatacaca tgttttgatt 300 attgatgaaa tttcaatgct taacgcagaa acctttgatt tattacatca tttagcatgt 360 aaaataaatc aatgcaatga cgaattgttt ggtggaatac aagttattac atgtggtgat 420 ttccgtcaac tgccacctgt aaaaggtgaa tatgttttca aacaagaaaa tattgaattt 480 tttaacgctt taaacgaaat acgaattgga aaagtatctg ataaaacgaa tccattattt 540 caaataccta ttagtataaa tattaaaatt ggcgctgtat tcatgttagt aaaaaatata 600 aatgttgaag aaggtttatg taacggaaca attggtattg tatcatttat agaaacagat 660 ggtgtttggg ttaatattaa cggtagagaa tttaaaatcg aaaacgtgag agaagacatt 720 ttggattgta ctcatagtat tattgcatct agaattggtt tacctttaca attagcattt 780 tctttaactg ttcataaagc gcaaggttgc acgttaaata aaactgtttt gaattttaac 840 agtaaaatat ttattatgtc gttaccttat gttgcattat cacgtgttaa agatttaaat 900 gatctttatt atcgagaaat attgtatttt gtaaatgaca attataaaga tgaagattat 960 attgaagaaa tcgaattaaa cgatcaaatt caaacacctg aagaaattac ttatcacatt 1020 aatcaaaaaa cacaagatag ttatttgatt gaatcaaata aagcgttatc atttgccgat 1080 ttttttcaaa actttttaat cggttataat attgtaacac tttgtgctaa atctacagaa 1140 gacgaaaatg atagcggatg gagaataatt cgtttatcta gtttcaaagt aaaaacaata 1200 agtaatttaa ctgaaaaatc aatgatgaaa cttcgaaatt atgaacttgc tggatataaa 1260 agagttgctg aactgagtcc agaagaatta atgataagga gagaacgtaa acgtaaacga 1320 aagaaaaaag ttgctgagct gaatccagaa gaattattaa aaaaacgaga acgtgaacag 1380 ctattaaaag tagtatacga gagtgctaat ataactaaag aagatcaaaa tcctcaaaga 1440 gaatttttaa acgaagatga aagaaaagaa gctaacgaac gtaaaaaatc actaaataca 1500 ataaaaatat gcttatggtg ggaaaaattt gaccgtactc ttataacaaa cgggttttta 1560 agtgattatg taagaataaa ctcgttagac ctagtcgaac acatgaaatg tttaagtcag 1620 ctcattatga acgaacaaca aattaacata tggtttgaaa atgatcctat agaattacta 1680 gaagattctt atattttaat acgaattttt caaaaatgta tcaacgaagt taacgtgcat 1740 tggaaatatg aacgtggaat gggaataaaa gattctatta catttttaca tctcatgtct 1800 cgtttaaatc atccgtctgc tcatttatgg tttcattgta tgttggaaca gttaaataga 1860 gaatttgaaa aaaaaagaac actaaaagat ttaattcaag atgataaagg aagagatgac 1920 gtcttaaaag aaagttattg tttgttatca tgttttgaag attacttggt tattgatcat 1980 tgtcactttt caggcacagt ttacggattg gcacacgatt attgcaacaa aaacttggca 2040 cgttatagta gtaaattagc ttgtcctata tttgcccaca atgcttcttt tgataatcgt 2100 atcatgattg ttgaaggttt aaaaaaaata ttagatgttc ctttatatag ttacgaagaa 2160 gatactgagg acaaaccttt taacgctttt ttaaagtgta gcaatatgga aaatttgttt 2220 gttatttcga aagggtcttt attacacatt tatacagttt tagatactgt ggttttgggg 2280 tctgtaatga ttgattttga cgaaagaaca tttcaattta ctcaatttcg aatgttacct 2340 catttaagtg tttttagtta ttgtagtaaa ctcaattcag ttatgagtca aatacaaata 2400 caatatccgc cctctgagga acacgaacgt atgattgaaa aaagtgttag aggtggcatg 2460 tcttcaaatg gttcacaaaa atatgcaatt gatacagatt attttgagaa taaaattaaa 2520 gaacttggat tgaaaagtga catcaaagaa caagtaattg aaaaatatca tcgcgtcaat 2580 cctgacggtt tttcagcaag tgctttagtt cattgtcaaa tcaaaatgaa acctgaatat 2640 caagataaag taggaggctt ttcacctatg gtcgaaaaag atattatacc tttagaagat 2700 tattctccta ctgaaattgt agctaaaaga atataa 2736 <210> 8 <211> 1254 <212> RNA <213> Hydra magnipapillata <400> 8 acaaacaagt atattaaact taacatcaag ctaaagaaat tgactaatca aaatatttaa 60 cgacttaatt ttttataaac tatgaaatat atatcgattt ggatcttata tatttgcttt 120 atgagtaaat cggttatacg tgcttcagtt aatcaatcag aaatagctaa tacaaataat 180 cgcctcgatt tgaaaatgtc gtcttcaatt ggtcgtgtaa agcgaaatct tgaatcgata 240 aaccttttcg atcagcaaga aaaaagacaa acagtaatgc ttaaattttg ttcaaactct 300 aacttttcaa ataaaactat ggatgaacac atgatttaca ctgacaaaga aagtcttctt 360 tactgctata tacctaaagc tgcttgtaca acttggaagc gaatgtttca gatatttgac 420 ggaaaaatgg atttaaatca agtgatggct gttgaaaaaa atgcagtcca taagctgcat 480 tataacaatt ttacaactct tgacgctgca caaaaagttt tccgtgagaa aaattattat 540 tcatttttag taagtcgcca tccatttgaa agattgttgt cagcgtatag aaataaattt 600 ttggaccctt acacaacgca ttatcaaaaa aagtatggtg cagaaatcct tcgtttatat 660 cggaacgatc ttacagaaga acagtattta aaaggagaag gggttacatt tcgagaattt 720 atcaagtatg ttatatcagg aaaacctttt gataaacatt ggcgtcctat gacgcaacta 780 tgctctccgt gtcgttttaa gtacagatac ttgggtaaaa tggaaacatt atttgaagac 840 gccaccgcaa tactcaaaaa cgctggcata tctcaaaagt ttttgttcct ctccaattca 900 agagatcgtt ataagccaat ttcaacaatt gacatgaaaa gtcaatatat gagtttgaaa 960 gcttcagaaa ttagaaagct atactacatg tataaagatg attttctcgc ttttggctat 1020 actgttccat attacattga agagcttcta gcaaccatcg acaatattta aatataaatg 1080 atataatgtt ttatcaacaa aatagttgta ttactagttg tttttcattt ctagcatttt 1140 atattgtttt aaatttttat ttatgatttt ataaaaatac ataatatgag aactgttttt 1200 tttttttttt gcttacgatt attaaaattg taacaaggta ataataaaga taat 1254 <210> 9 <211> 633 <212> RNA <213> Hydra magnipapillata <400> 9 atgcatatta ctggcattga aggagtcgag gatatgattc gacttggaga attaaatgaa 60 gctggaatac tgcataattt attaaaaaga tactatgaaa acaatattta tacatacact 120 ggttctatcc tggttgctgt aaatccatac caagtgtatc ctatttatga tgcaaattat 180 attaaaaagt accagggaag aaaaattgga gatcttcctc ctcatatttt tgcaattgct 240 gatggctctt attattatat gcgaagagaa aaacaagatc aatgtatcat catcagtggc 300 gaatctggag caggtaaaac agaatcaaca aaattaattt tacaatatct tgcaacaatt 360 agcgatgagc gtaattatca catcttttat tatatgctac ttggtatttc gccagcagaa 420 aaacaaaagc ttcttcttac tcgggctgaa gattatgctt atctgaatcg tggtggttgc 480 cttacatgtg atggtattga tgatgctgaa gaatttggaa ccattcgtgg tgctatgaaa 540 gtccttttgt ttacagacaa tgaatcatgg catatattta aacttttggc tggtgttctt 600 catttaggaa atataacttt caagagtatg taa 633 <210> 10 <211> 432 <212> RNA <213> Hydra magnipapillata <400> 10 taatatttca attataagtt tcaaactgac gttctaaagt tcaacactta agaaaaaaat 60 atatatattt atatatatac acaaaaatgc caaagtgtcc taaatgtacg aaagaagttt 120 attttgctga gaaagtcaca tcgttaggta aagattggca ccgtgcttgt ttaagatgta 180 caaaatgtaa taaaactctt acctgtggta gccatgctga acgcgatgga tcgccatact 240 gccacaatcc ttgttatagt gcattatttg gtcccggtgg atttggccgt ggtggagcgg 300 aatcacataa atattgatgt tacaaaacag tttatattgt tgctactatc attgtaaata 360 aaaaaatgca accataatat ttaaacaaag tattagaaaa gtagtactag aaaatataaa 420 gaactttagt ta 432 <210> 11 <211> 784 <212> RNA <213> Hydra magnipapillata <400> 11 ctaaatagct ctaacggtat ttattataat attgttgaac ttttttactt ttagttgatt 60 ttatagaagc tttttacagt gcacaaaaaa agaagaacta tgagtgggga aaaaaaataa 120 gaatacaaaa taacttatca taatggagcc attattaatc atattaattt ttattatagg 180 gaacggtata tgtttacaag tcacatcgat aaacttaaac aatcattcaa ttgaaataaa 240 cgcctcccga atttttccta acgagtttac acatatagaa aaagataaag tgaaagaagg 300 ttcgtctcgt tcagaaagat acaaaatgca aaatggtctt atacttcttc atgctcaaag 360 accgcacaca gtttctgatg taaagaaatc tcaattgacc gataattctc agagtattaa 420 ttaccacgtt cgcaaaaaag aacaaaataa cgaaacggta tttaaaatat tagaatttaa 480 tcgattcgga gttaatataa ctattaagaa aaatgatttt atacaaataa acaataattt 540 attaaaacac atttcaaaga atgatacgaa tatttttcaa ttggctaaca gcacagaaaa 600 ctgtttaaac ttaactgaag gtaataaaag tttagtttgg gaaagtgatc aaagtataaa 660 aaatgataaa attcaaagac aaaatacaat tactcaacaa atagtaaaac taaacaacgc 720 tatagaagat gcgaaaccaa atgctcgaat aaaaagagga aaagctggta gagaagccaa 780 ataa 784 <210> 12 <211> 589 <212> RNA <213> Hydra magnipapillata <400> 12 ttatcagctt tgaagaaaat gtcagccaag gtgaatgcaa ttgaacttgc tgatcttcct 60 aaccaagagg tacacgttga ggttcaaagt aatcaagatt ggaaagcgta cgatgactca 120 aaatatgatt ctcctgtgct aaatcgcttc gttccattcc caacatacta ctatattaga 180 tggaaattac ttgcattttt ccatatccaa cttttgtttg gcatcgttgt aggagaggta 240 atactatttt taatcttggt gggagggtta gctggttggt tggcagctat aggaatgtca 300 acaacaacta gcgtaacaag tgcaatcaat cagacatatg gtcgacaaaa aaatcctact 360 aaggagttag ctgaggaaac cggaagtata gctgcaatac cgctaatttt atgttttgct 420 ttggcgtgtc gaaattcttt atggattgtg cttgttggcg ttccgtttga acgtggatta 480 ttttggcata agctatgtgc ttggttaggt gttcttgttg gcgcgtggca tggctacgta 540 tctcaagagt ggaatgtaac tggtttggtt ctcacaggag caatgggag 589 <210> 13 <211> 702 <212> RNA <213> Hydra magnipapillata <400> 13 ctgtttgaat cagaatcata tggagaacgt cgtggtgcag cgtttggact agcgggcatg 60 gcaaaaggtt taggcattct atcattaaaa caacacaata ttatcagtac tttaaatgaa 120 tacattcaag acaagaaggc tgttatgaga aatttgagta atcatggtgt aaaacttgtg 180 ctaccatctt tacttaaagc attagaagaa gaatcatggc gcacaaaaac aggcagtgct 240 gagcttttag gtgctatgtc attttgtgct cccaaacaat tgtcatcttg tttacctagt 300 attgtcccac ggctaacaga aatacttgct gactctcatt taaaagtaca gaaggctggg 360 caacaagcac ttagacagat tggtggtgtc attagaaatc cagagattca agaaatatca 420 tctattatac ttgatgcatt atctgatcca aacaaaaaca cagtggcttg tcttcaagca 480 ctactaaata cttcatttgt tcacttcatt gatgctccat ctcttgctct aattatgcct 540 acactagaga aagcattaga tcaaagaccc acagaaacta aaaaaatggc tgctcaaatt 600 cttggaaata tgtatgcatt aactgatcca aaggacttga ctccctacct gccggctgtt 660 gtaccaggtt taaaaaaatc tttgttagat ccatcgcctg ag 702 <210> 14 <211> 582 <212> RNA <213> Hydra magnipapillata <400> 14 gttactctta aagtagaagg tcaagttctt gaagtcagtg gaaagcatcg taacgaaaat 60 gaaaatggat ttgagtctag tgagttccac agaaagtaca ctattcccga tgacgtggat 120 gcgacagccc ttacgtcaaa catcagtcaa ggcggcgttt tgcatataga agctcctaaa 180 aagctccctg catcttctgg agaatcaacg aaagagactt ttaaattctc tttagatgta 240 caaggcttca agccagaaga aatttcgatt caagtaaaag gtagagattt agtcgttcat 300 ggtgaaacaa aaactgaaaa cagcggtgaa cacggttcaa gttaccacca caagcagttt 360 accaaacacg tagctttgcc agatgatgta gatccatccg aattatgttc tcgctatacg 420 aaagactcta aattaacgat cgaagctccg cgaaagcaac tgcaagctcc gcttaaactt 480 gaaatcaaaa tggagaataa agtggtgtct tttacaattg tttgtagttg tgttgtagct 540 atttgtaatt ttatttttta cactaatagt atgttacgct aa 582 <210> 15 <211> 2436 <212> RNA <213> Hydra magnipapillata <400> 15 ctacctgtct gtaacctttt gcagatcaag taattcttca aacttgtaga gttctttaac 60 acgacttctt gcgaaataag ctatacatta aaaaatggtt gaaggtggtg aaattgaaac 120 tttcgctttt caagcagaga tagctcagct aatgagcttg attattaaca cgttttactc 180 taacaaagaa atttatctgc gagagttgat ttctaatgct tcagatgcgt tagacaaaat 240 ccgttactta tcactaaccg atccgactgt tttggattct ggaagtgaat taaagatcga 300 cattatacca aacaaggagg aaaaaaccat cactattttt gacaccggta ttggtatgac 360 aaaagcagat cttgttaaca atctcggcac tattgctaaa tcaggtacaa aagcatttat 420 ggaagctctt caagctggag cagatatatc catgattaga cagtttggtg tcgggttcta 480 ttcagcatat cttgttgctg ataaagtgga agtgataaca aaaaacaatg atgatgagca 540 atatatatgg gtttcttctg ctggtggctc atttactgta caaagagaca cagtcaatga 600 accacttggt agaggaacaa aaattatact gcacatgaaa gaagatcagc tagatttttc 660 cgaagaaaaa aaagtaaaag atatcattaa aaaacacagt cagtttattg gatatccaat 720 caatttgaga gttcagaaaa ctagagacaa ggaggtttct gatgacgaag ctgaagatga 780 agagaaaaaa gataaatctg aagaaaaaat ggaggatgaa gatgaagatg aacctaaaat 840 agaagatgtt ggtgatgatg cagaagctga gaaaaaagac aaaaagaaaa aaaagaaaat 900 aaaggaaaac tatactgaaa tggaacaact caacaaaact aaaccgctgt ggactagaaa 960 cccagatgat attagctctg aagagtatgc tgatttttac aaaagtttga ctaatgattg 1020 ggaagagcat cttgcagtaa agcacttctc tgttgaaggc caacttgaat tcagagcaat 1080 cttatttgtt cctaaaaggg ctccatttga cttgtttgaa aataaaaaac aaaaaaattc 1140 aattaaatta tttgtcagaa gagtttttat aatggaaaat tgtgaggaag tgatgcctga 1200 atggcttaac tttgttaaag gtgtggttga ctctgaggat ttgcctctta atatttccag 1260 agaaatgttg caacagagta aaatattaaa agtcattcgt aagaaccttg taaaaaaatg 1320 cttagaactt tttgttgaga tttcagagga taaagataat tataaaaagt tttatgaaca 1380 atttagtaaa aacattaagc taggtataca tgaggactcg caaaatcgtt ctaaagttgc 1440 tgatttattg agatatcatt cttcagcatc tggtgatgat atgacatccc taaaagacta 1500 tgtttctcgt atgaaagaga atcaaaaaga catttactat ataactggag aaagcaagga 1560 aattgtttca acttctgctt ttgttgagaa agttaaaaag aaaggttttg aagttctata 1620 tcttattgac ccaatagatg agtatgctgt tcaacaactt aaagaatatg atggtaaaaa 1680 attagtttgt gttactaaag agggattaga attgccagtt agtgatgatg agaagaaaaa 1740 acaagaagag ttaaaagcta gttttgagga gctatgcaag gttataaaag atatattaga 1800 taaacgtgtt gaaaaagtca ctgtctctaa tcgcttggtt gattcaccat gttgcattgt 1860 tactagtact tatggatggt cagcaaatat ggaaagaatt atgaaagcgc aagctcttcg 1920 tgacacaagt acaatgggtt acatggcagc taaaaagcat cttgaaataa atccagagca 1980 tagtataatg gttgccctta aaaagaaagt tgatgcagat aaaaatgata aatcaataaa 2040 agacttgatt gttttgttat atgaaacatc acttctgtcg tcaggttttt cgcttgagga 2100 tcctcaaaat catgctgctc gcattcatcg tatggtcaaa cttggtctag gtgtcgatga 2160 agatgaaagt gccgtagagg aaatggcaac agacgatgtg ccacctctcg aaggtgatcc 2220 ggaaaaagac gaagataaag ctagaatgga agaggtcgac taaaacgcta tgttctgcaa 2280 aatgtaattt caacgtgtgg tgtatccaaa tggtccccta ttttttgtac agtttttagt 2340 taacccgtag gtgagattca atttattggt ttcacgtgta aataaatcac taaaatagaa 2400 tttttaatta gaagacctat aaaccaataa atcaac 2436 <210> 16 <211> 880 <212> RNA <213> Hydra magnipapillata <400> 16 acgcaaccaa gcagattagc attgttcgag aagagagatt tatactgact agagaaaaaa 60 atgtctttgt ggcacattcc tgttcacaaa tatttttctt ccgatccatt tttggaagat 120 ataatggaaa ttacgtttcc tcccctaaga tattatccat tatttaatgt tggaacgggc 180 gctcttgcga aaaagactaa caaagaaaac ggattcgttg taaatcttaa tgtcaaacac 240 tacaagccag aggaagttac tctgaaagta gaaggacaaa ctcttgaagt aagtggaaaa 300 catcgtaatg aaaatgaaaa tggatttgag tgcagtgaat tccatcgaaa gtatactatt 360 ccagatgacg tagattctac agcacttact tcaaacatca gtcaagatgg tgttttgcat 420 atagaagctc ccaaaaagct acctttaacg tctgattctg gagaatcaac aaaagagaca 480 tttaaatgca ctttagatgt gaaaggtttt aaaccagaag aaatttcgat tcaagtaaaa 540 ggtagagatt taattgttca cggtgaaacc aaaactgaaa atagtggcga gcatggtttg 600 agttttcacc acaaacagtt taccaaaaat atctctttac cagatgatgt cgatccttcc 660 caattaagtt ctcgctatac taaagacttt aagttggcaa tagaagcccc gcgaagtcaa 720 gttcaggctc cactcaaact tgaaatcaaa atggaagaat aaactttctc gaatcattac 780 ataaaaagat tttataaatt agttgtgtag ttatattctt tttacatatt tttacatttc 840 aatagatgct gtaaattatt tgtaaattat taaaataaat 880 <210> 17 <211> 1299 <212> RNA <213> Hydra magnipapillata <400> 17 atgagacgtt ttagatgctt tccattaagg tcagcaaaaa ttaacattct tgccagtgga 60 ttatccccag cttttctccg tataggtgga acgccacaag atttcttgac tttttttgat 120 agagcaaacg aaagtattga tataaaaacg tcgtgtacac caaagtttga gaattggcgc 180 aaactaaaac cttttcagct aagttctcaa tactttgaag aaatcggagt gtttgcaaag 240 aaaaaccatt tagaactgat ttttggttta aatgctttga aaaggtattc aaatatgtcc 300 tgggacaata ataatgctgt tgaaataatg aaatttatga aggataaaaa tctcacaact 360 atatggactc tcggaaacga acctaaccgc tttaaaaagt atggaacaaa agttagcata 420 agtccacgtc agttagcagc tgactttctt aaactacgtt taataacaac tcataaaatt 480 tatggaccag atatatctca tcctacttgc aattcattaa agtatttaaa aaattttctc 540 agaaataagc ctcttcttga tgctgtgacg tatcaccatt acagtatgca ccaaaacctt 600 tccacaatgg agatgtttct taacccgttt tatcttgata aatttcatga agaaaatggt 660 tggatacgct cattaattac ttcagaaaaa ttgaatgttg atctttggtt gggtgaaacg 720 ggaagcgcta gtggcggagg tgctcagaat ctatcagata cttttgcatc aggattctta 780 tatttaggaa aactaggaac tgccgctgct gattgtcata aagttgttat tagacagact 840 ttatacggag gatattacgg aatgttagac cctgtcacac atgacccgct gcctgactac 900 tggagttctc ttttgtataa aaaacttgtt gggcaagttt tattatcaca tcaatcgctt 960 aaaaacggtt atttacgtat atacacatac tgtgctcgaa attcatctac gcaagtagtt 1020 ataatggcta ttaattttaa cgttgataat gaagcagtct tgcaagtaaa aatgtttggt 1080 tatagtttgg ttgaaaagtt tatacttacg gcgccaaata aaaatcttca gtctaaagta 1140 gttttattaa atggagaaca gttaaagtta aaatcggatg gaagtcttcc gagtcttaat 1200 ggaacatttg ataggcagcc atttttttta cctcctcaga gttacggttt ttttgttttg 1260 caaaacgccg agttggatga ttgtaaagac actttttaa 1299 <210> 18 <211> 744 <212> RNA <213> Hydra magnipapillata <400> 18 atgactcaag agtgtattga cttgaaagtt gtactagaaa ttccatgggg tttgaataat 60 actcttgaaa atactccagg agaatttaaa gaacaacaaa acaatgatgg cgcgtttctt 120 gatggaggct tgggcaccaa cgaaaatatt tattttgtta acgcgaaaag aaaagacgag 180 tcaagacttt taacggaaaa cgaagaaaaa aatggattcg caaaaagacg acatcgcaga 240 ggatctcgca aaaataagcc aagaaaacca cgacaaagat ataaaccttt taacaaacta 300 acttgggatg aaaagcgaca gttagatgaa agagacactg taaaagcact aagacggaga 360 gaagaactgg tcttacaaaa aggacgtccg attgcgcctt acaacactac gcagttttta 420 atggaagaac ataaccctgg cgaagaaaat tttcaaaatc aaaatacaac tttacaccct 480 actgctgact tatccgaaga ttcttgtagt tcttcatcga gcgatgaata ttttgttaaa 540 gactttgacg aattttacga gcgggtacat attgatactt tgaattctta ttctaaagaa 600 gatcttataa aatatgtttt tgaattggaa gcgcggattg aaaaatttga aagggaaaac 660 aaaaacagtg ataatttaaa agaagaaaac tataaactac tccaggaaat caaagattta 720 aaagctgaaa aagcgaaaaa ctaa 744 <210> 19 <211> 1410 <212> RNA <213> Hydra magnipapillata <400> 19 taattttttt taagaaagta aagaagtaaa aagtagttga aactgctgaa caagaaaaaa 60 gttgctctgc aatgctttaa tgatttgatc aaaaacttat ttatagagga atttgtaacc 120 ttcagttaca gattttcaat tggaacaatt tcttgtcagt gacggcttga gcaactttgc 180 atcttctttt aatatagtca attaacaatg ttaaacttcc aagatattgc acaaattact 240 gtagaagcaa tacaagaaaa aaatgaactt cctaacgaag aggaaaaaaa tactcaacac 300 cctcgagatt tacgaaataa aaagataaaa gaacatatat cttacatgat tgacaactct 360 tcatttcacg gactatctta tatatttgat aaaagacatt ctattcgccg tacaatatgg 420 tttttcataa caatagctgc gtttgtttac gccatgcaaa aagtttatga aagtacaatg 480 aactactttt cgtacccgtt ttataccgct cgtatgagaa tgtatgtgaa ccagataaat 540 tttccagctg tatctttctg taacttgaat gatataagat ttagtgtaat gaacggaaca 600 attgttgatg acgcaattat aaataataat caagaagcaa atataacagg ggaagaaatg 660 agaactttta ttcaagcagc tagacataca ttaaaagaaa tgttagtaga ttgtgacttt 720 gaaggtaaga aatgttctta cgaaaacttt acagaattta gttggatgca gggagaaagt 780 tgctttactt ttaattctgg caaacctcct cacactctgt tgaaggtgaa cggagcaggt 840 ataaatagaa gcttgaaact caccatcaac gtccaacact atgaatacta tagagataaa 900 atggatgctg gtattcattt aattttacat ggacaagatg agacaccggt taaaatgcga 960 ggtctacatc ttccacctgg gtttacatca tatattcaaa tagagaaaaa gacgatcata 1020 aatttagaag caccgtacaa aacaaaatgt ggttcagtag agttaaaata ttttgatagc 1080 tactcgatgc atacttgctg gcttgaacaa cttacagacc atgtttacaa agtgtgtaaa 1140 tgtaaagatg tttttatgcc aggagatatt ccgatatgtt cgtttgatga agcatttaac 1200 tgcatgtggc ctcaatggga aacatttgat aaactgaaat tatatcattg tccgttacct 1260 tgtaaaattg actcgtacgc tgtaagtctt tctcgagctc tatttcctac ggctcgttat 1320 gctagtagtt tggcaaatga actccgaaaa catcaacatg tggccatgaa tttaaaaagt 1380 aatgctgatg aactagcttt tatgaggtaa 1410 <210> 20 <211> 909 <212> RNA <213> Hydra magnipapillata <400> 20 atgaacactc cctcccgcgt caagatcatc gacgtcggcc cgcgcgacgg gctgcaaaac 60 gaaaagcaac ccgtgcctgc cgccatcaag gtcgagctgg tgcaccgcct gcaggatgcc 120 ggcctcaagg aaattgaagt cactagtttt gtttctccga agtgggtgcc gcaaatgggc 180 gacaacgccg aggtaatggc gggtgtgcaa cgccgcgatg gcgtgcgtta ctcagtgctc 240 acgcccaaca tgaagggcta tgaagctgcg gtgttgtccc gacccgacga aatcgtggtg 300 tttggcgctg cgagcgaagc tttcagccag aaaaacatca actgctcgat cgaggagagc 360 attgagcgct tccgcccggt ggcccaggcc gccaaagaag ccggcatcgc agtgcggggc 420 gccatctcct gcgcggtggg ttgcccctat gagggcgaca tcgctcccga acgtgtggcg 480 caggtagccc aactgatgcg ggatatcggc gtggagcacg tgggcgtggc cgacaccatt 540 ggcgtgggta ccccggtcaa ggtgcagcgc gccttggaag ccacattgca gtactacagc 600 gtggaccagg tttccggcca ctttcacgac acctatggca tggcgctggc caacacgctg 660 gcgtcgctgg aactgggtat ctggcacttt gatacctcgg tggccggatt gggcggttgc 720 ccctatgcca agggcgccac cggcaatgtg gcttctgagg acgtggtgta catgttgcac 780 ggcatgggta tagaaaccgg catcgacctc gacaagctga ttgacgcggg caaattcatc 840 agcgattttc tgggccgcaa gccgcattcg cgtgcggcca atgcgctgct taccaagcgt 900 ctgggctaa 909 <210> 21 <211> 864 <212> RNA <213> Hydra magnipapillata <400> 21 atgtctggtc gtggtaaagg aggaaaaggt ctcggcaaag gtggtgccaa acgtcatcgc 60 aagatattgc gtgataacat tcaaggcatc acaaagcccg ctatacgtcg tctagctcgc 120 cgaggtggag tcaagcgtat ctcagggctt atctacgagg aaactcgagg tgttctgaaa 180 gtctttttgg aaaatgtgat tcgcgacgct gtgacttaca cggagcacgc caagagaaag 240 actgttaccg ccatggatgt tgtctacgcc ttaaagaggc aagggcgcac attgtacgga 300 ttcgtagcac aactacttgc tgctcgtgta cttggtaaca gctttggtgc cttcgctcac 360 tgcaaatcta aaatgtcagg acgtggtaaa ggaggtaaag ctaaagccaa ggccaagaca 420 cgatctttca gagccggtct tcagtttccc gttggtcgcg ttcatcgatt tttacgcaaa 480 ggtcattacg ccaaccgaat cggatcaggc gctccagttt acttggctgc tgttttggag 540 tacctatccg ctgaaatctt ggagttggct ggtaacgctg cccgagacaa caagaaagcc 600 agaattgttc ctcgtcactt acagctcgct gttcgcaacg acgaggagct gaacaaactg 660 ttgtcgggtg taaccattgc aagcggtggt gtgctgccaa acattcaagc cgttttgctg 720 cccaagaaga acgaaaaact tccaaaacca gcagcagcca agtaaagagt ggagctacgc 780 gagcacgcac taaaacaaaa aggctatttt catagccaca catttgtcaa aaacattttt 840 gcgtgaacga aaacgactcc agaa 864 <210> 22 <211> 657 <212> RNA <213> Hydra magnipapillata <400> 22 gtcatttttt attagaataa aactaaacag cgcataaagt gtttaaatac atcattcatg 60 taatacggcg ttaagatggc tttattaaat attatacaat gcgtgctaac ttgtgttggg 120 atagggtttg ctatatcctc gaccgtagga aacagttggt ggaaacaaga actttctgat 180 aattctatac atgctgggtt atggaatttg tgtaacctct acacttgtgt gttcatgagt 240 aacgtaccaa atttcttaac tgttaccaga gcctttatga ttactggttg tgtgtcatac 300 tttgttgctt ttgtattaag ctttctaact tattcaaaga aaataaagaa tcccaaaatc 360 tctggtacat tactatgtgc aacagctata ttcatggcgg taggaataag tgtgtataca 420 aataataccg atggacttgt atacagttgg tcttacattt tcggatggtg ttcatttgta 480 atttctattg tgtctgcaat tatttgtttt gtgatgaaag tggaaacaga gtatttacaa 540 atctagtttg gattcttttt tttattgtaa aaattaggct ttttcaaatc atttttttat 600 tctttaaata tttttgaaat tctttgttta agtatgtttt agctacattt ttgttgg 657 <210> 23 <211> 238 <212> RNA <213> Hydra magnipapillata <400> 23 atggccactg cacataacat atcaatagat gtttgtttaa gcagcactat tgggagtcgt 60 atagtgcgtg gtcttgattg gaaatggggt aaacaagatg gaggtgaagg ccatgttggt 120 acaataagaa gttttgagag taatgaagaa gtggtggttg tttgggataa tggcactgca 180 gctaattata gatgttctga aaattatgat ttaagaatac tagattcagg gcctagtg 238 <210> 24 <211> 748 <212> RNA <213> Hydra magnipapillata <400> 24 cggtcgttgt ttacaaacca tggtgcaata acataacata aaactagcgt tttgcttttt 60 agaattattt ttaaatctaa gttaaataat aatttgagtt aaggacaaag taaaaatgat 120 attcgaagag tatgaaaaaa tggccgattg tatcaataaa tttatcggtt ctagtgaatt 180 ggaaaaaact tcttggattg ttttagaaaa aatacacggg gcaaatttca gttttcatac 240 agacggcgaa tgggtacaaa tcggtcgtcg aagagatttc ttaatggaag gcgaaaactt 300 ttttaaccac attaccgcta gtttcatgaa tgattacccc gagaaaatga aaactattta 360 ccgtatggtt gaaacatcaa ctggtaaaca aatcaaacaa gtttctatct atggggaact 420 gtttggaggt tactattcca atgtaccgac aaacggaaac caaaaaccaa tacaaaaaga 480 aatacaatac tgtccagacg tcaggtggtg tgctttcgac attggctaca ggacacaatt 540 cgaagaaggg ttaaacggta cgatgctatt atttattttt gaaatgtaaa tcaataaata 600 gtataatgac atattaaaaa agagtagttg atctaagaac atcctactga ttgtacttca 660 taatagaaca ttaaatttgt tattagataa atatatcaat atctaaaaat ataaaaataa 720 atgatatata aattacttta ttattagc 748 <210> 25 <211> 313 <212> RNA <213> Hydra magnipapillata <400> 25 aaaataaaaa tattagctct gtatgcaact aataaaaatg tgctaatcat ataaaatgtc 60 actatgaaat gaagagcaaa gcattaaatt attcaaagaa catcagtcgt gtggcagtat 120 ttgtgtttct tttacaatgg aaaatgatga attgccaatt aagacaacac caattgaagc 180 tgattacatt atattttgga acaaaaagtt aggatctggt attagtggac cagtcagaat 240 ttgtcaaagt agaacaacaa atgaaaaatt tgcatttaaa tgcttggttg atcgtccaaa 300 ggccaggata gaa 313 <210> 26 <211> 1041 <212> RNA <213> Hydra magnipapillata <400> 26 atggttaaga aatatagtgg catgaatgca ggattgagag aaacagatcg taactgtatt 60 ttttctagct gtgtaaatcg tgctgtaaat cttgtgaatg tcgattgcgc tcaatcgaca 120 atgaagcaat tgcgtagttt ggaaaaactc aacaaatcac taccactcgc accaaatttt 180 tcaacagcac ttccaatcgc accaaatctt tctacaacaa taccaatcgc accaaatctt 240 ttaacagcac ttccaaccgc accaaatctt tcaacagcac ttccaatcgc accaaatctt 300 tcaacagcac ttcctatcgc accaaatctt tcaacagcac ttccaatcgc accaaatctt 360 tcaacagtac ttccaatcgc accaaatctt tcaacagcac ttccaatcgc accaaatctt 420 tcaacagcac tactaatcgc accaaatctt tcaacagcac taccaatcgc accaaatctt 480 tcaacagcac taccaatcgc accaaatctt tcaacagcac taccaatcgc accaaatctt 540 tcaacaacac taccaatcgc accaaatctt tcaacagcac tttcaatcgc accaaatctt 600 tcaacagcac taccaatcgc accaaatctt tcaacagcac taccaatcgc accaaatctt 660 tcaacaacac taccaatcgc accaaatctt tcaacagcac tttcaatcgc accaaatctt 720 tcaacagcac taccaatcgc accaaatctt tcaacagcac ttccaatcgc accaaatctt 780 tcaacagcac tttcaatcgc accaaatctt tcaacagcac taccaattgc accaaatctt 840 tcaacagcac ttccaatcgc accaaatctt tcaacagcac ttccaatcgc accaattctt 900 tcaacagcac taccaatcgc accaaatctt tcaacagcac ttccaatcgc accaaatctt 960 tcaacagcac taccaatcgc accaaatctt tcaacagcac taccaatcgc accaaatctt 1020 ggcaataagt tttcatttta a 1041 <210> 27 <211> 597 <212> RNA <213> Hydra magnipapillata <400> 27 atgacgttag agaggttttg caagattgga tttaaacaaa agcagttaga agaagagact 60 aatataaata ttatgatgaa aactcaacta ctgattggat ttgctctagt ggttgtttct 120 caatgtatga tcataaagta caaaaaatgt tcatcaccag catcaagtgc cgttgttggc 180 gatgttgtaa tatctccatg cgataatcaa ccatgtcaat ttataagggg tggaaacgcg 240 aacattcaaa ttcattttca ggccaaaaaa gataattcca acattaccac tatagttaaa 300 ggcaaaattg gtccactttg ggttcccttt cccctatctc aaccagacgg atgcctcaat 360 gacggaataa tttgtcccgt aaaaaccgac caacaatacg tttattcgta tgatttacct 420 atttccaagt cgtatccggc gatttccgtc gtagtaagct gggagttgca agatgaaaac 480 ggtaatgatc tggtatgcat ccaatttcca ataaaacttg tataaaactt caaattgtta 540 aaagtatttt atctttaagt taattattga aataaattat ttctcgaggt ataatta 597 <210> 28 <211> 1393 <212> RNA <213> Hydra magnipapillata <400> 28 gcgattatgt taatttacca gaaaaaagaa aaaaagtgaa tcaattaaat aaaaaaaaaa 60 gcaaagtaat catgactaag ggtttacata gttatataaa aggctcttat aaaatgaagc 120 aaaatacaac aactaagtta gattcaactt tgtctgctgg agaataagtt taaagatgtc 180 tttatttgaa aaagaagtac acatgaaagt ttattatgtc gagaatcaac ataataatga 240 aattcgaaag ttttcagtag aaaatcgaat ggctgctaat tacaaataca ttttggataa 300 gatccgccaa gtatttacaa atttgtatga aaaagatctt gatttgtttt ataaagataa 360 agaaaatgat ttcatttcaa tctcctctga tattgaattc cagcaagcct ttgagagcat 420 caacaatggt tgccttaagc tctatgtaaa aaagaaattg acaaagccag ctcaatcaaa 480 taaagaacat attggagtta gttgtgatgg atgtaattct aagatatatg gaaatcgctt 540 caaatgcact caatgttttg actttgattt gtgctcatta tgctacaaaa aaggagagca 600 cccatcagac catgaaatgc tggttatcaa agaacctcga tcttctaagc atatgtgtta 660 cagtcagctt ccgttttcac attgctggga aagatatgca cacatgaaca cgaacaattc 720 ttgttctaat aaaaatgcaa gcaataatga agaaaataaa cctactgcat ctgatcaaaa 780 gtttggggta cctgctgaac aaatgcatgc tttggttgat tgttttataa aacagtgcaa 840 tcttaaaagc aatgaaagaa atagtgataa agatataaat agttcaaaca atgaagtatt 900 cagaaataac ggagacatga accaggctaa ttttccagat aaaaaagatg tatcattaga 960 aaaccagctc atcaataaag acaaggatct tgtaaatcct tcaaactcat tgtgtcagac 1020 taatgttgaa tccgtaccac atctagatcc aaacaatgtt tctgaagtta taaaagaaca 1080 aataaatccg cttgagtctt tgtgtcaaca aacctctgag ttgcaggttg atagttcaca 1140 agaggggctt gggaatttta taggacgctt atttagccac cttgtaactc aaaaaccacc 1200 aataaacata gccaatgatt ttgaacttgt tgataaagaa agtaaagatc atgaagaatc 1260 aaagcttgaa agatctctta gacaaatgga agcgatgggt tttgagaatg aaggcgggtg 1320 gttgcgtcag ttgttaattt ctaaagattg taacattgat aaagtgcttg atgctttaag 1380 tcctgccaaa tag 1393 <210> 29 <211> 1435 <212> RNA <213> Hydra magnipapillata <400> 29 tgagaatgat gtttgcaata aaaaaagttt tgcaatgagg aatagaagta caaaaacgaa 60 aaataaaaat catgaaaacc atatgactga taatgttggt ttaccttggt caactaaaga 120 aaagttgttc atatcagttg cacttagact tttttcagta tttttaacaa gaacattttt 180 tgttccagat gagtattggc aaagcactga agttgcccat tataatgttt ttggatatgg 240 ctacttaact tgggagtgga agcaaaaaat tcgttcatat ttttatccat ttttgtttga 300 gatctatttt cgaacaatca agctttttgc aatagattct cttttcttta ttgtttatgg 360 ccctcatgtt atgcaagcat tgctcacaag ttgttgtgat ttatctgttt attactcggc 420 atttaatttg tttggatcag acaaaattgc atcactagca tttttcaatc aaatctgttc 480 atggtttttg atttatacag gctcaagaac tctttcaaac actgctgaaa tgtgtttttc 540 tactttagga atattttatt tgtttgcaag tacatcaaaa agcaattttt ttttaagctt 600 attacttgga ggtatttctt gcattattag accaacatct gttatcatct ggtttccttt 660 agtattaagg gaaactatat acagcaagcg ttcaataaag aatattgcag ttaacttgat 720 atttgttaca tctgcattgt tgatgttaat gttaggtgtt gatttctact tttataaaca 780 atttgtttta acacactgga actttttaca atttaattta attgaagata tgagtcagtt 840 ttatggttca cacccatttt attggtattt ttttgaaggt cttcctgttg tttttggatt 900 tagtattgca tttatatttc ttggagccat aaaatgcaaa aaacaaaggt tctttctttg 960 gattattttg ctttatgtaa ttgctcacag ctttatttct cataaagaat ttcggtttat 1020 gctgccaaca attccaatag cttcttttta cgctggttac gggctcaatt ttttatctac 1080 tcttatatcc aaaaaaaagt tttacatgtt acttgttgtt cttgttattt caaacacttt 1140 tgtagcatta tacttttgtt tgatacatca acgtggatca atcgatgtga tgaaatactt 1200 gagtgcacaa tccagcagaa gtataaaagt tctctttctt atgccatgtc attctactcc 1260 atattacagc taccttcatc gtaatgtctc tctacaattt ttgacatgcc acccatcaag 1320 tgagttaagc tatgttgaag aagcagaagc tttttatttt gaaccaatat catggttaaa 1380 tgaaatagct gctttctcta aaactcatgt tgttttttat aatgtaatcc ctgag 1435 <210> 30 <211> 1350 <212> RNA <213> Hydra magnipapillata <400> 30 ggattatttt ggtactgtac agatgagtca aaagataacg aagaagattg tgtacatatg 60 catcatggca ttgatagcac attgcaaggc aaatctcctc ttaaaaataa taaaatatgg 120 gcttctgtat actttgtagt atatatcatt gtgtttatgc ttttcttcat caatgtgttt 180 gttggcttaa tcattctaac ttttcaaaag cttgctgctg ctgaatttgg tcttgaactt 240 gatcgaaata aaaatctttt taaagtcttt tggaatgttt ttgacgcatt aattgtgtta 300 agtggactat tggatataat gattaaagca ttatttccaa atatttcgtt tgacacaagc 360 atatttcgct tatttcgtgc tttgcgatta attaaattgc tacgaaaagt tcgttctttg 420 cgaattttac tttggacctt tataaagtca ttgcaagcat tacctcatgt tgtcatgcta 480 atttttctat tattttttgt gtatgctttt gttggaatgc agattttttc gcaaatttca 540 attgaaccag ctgataatcc atggggccaa ataaatgaaa acaaccactt caggacttac 600 tttagttcaa tgcaagtttt agttagatca acttcagggg aaaactggcc tctgataatg 660 aaggcttgtt ccgatggtgc tcgttgtgat tacagcatga aagcagttga tccttcatct 720 actcaatgtg gaacaaattt tgcttatgtt tacttcattt catttatatt tttttgctgc 780 tatttagtgt tgaatttatt tgttgctgtc attatggata acttttcatt tctcactgga 840 gattctagca ttcttggacc ccatcattta gatgagtttg tcactgtttg gtccgatttt 900 gatccatgtg ctagtggtcg catcaaatat actgaggttt gtgagttact tcgacaaatg 960 cagccaccat taggattggg tgcaaaatgt ccaaatgttc tggcatataa gcgtttagta 1020 cagatgaata tgattttgta tgaaaatggc acagttgact atacaggaac attttgtgca 1080 cttgtgcgta caggtctgga agtatatact gaaaatacaa atctaaaaag taatgatatg 1140 gaatttcgta agatgttgaa gatggagttt ccaaatatta caaagcgaac tttggacctt 1200 gttataccaa ggactcctaa aaattgcaag gaaatgacta tagcaaaaat ttattctgca 1260 aaacttatat gggagcatta taagagtata aaaagaagaa gtaatagaaa aagaaaagtg 1320 cagaggtgtg agttagaaac tttagattaa 1350 <210> 31 <211> 562 <212> RNA <213> Hydra magnipapillata <400> 31 aaattaaatg ccactaaaat tacttcgaaa tggtcattat gcatatgata aactctacac 60 tctacatata cgacttcttg ataatacagt gatgaacata aatgttccac ttgcttctaa 120 aggcaaagat tgtttacaga aaattgcaca atatattggt ttatatgaga ttgaatactt 180 tggactacag tttttgacaa agaaggaaaa gcttcattgg gttgatcttg ataaatgtct 240 tagaaaacag cttgatcgac ataaaagttg caaggggaca gaagctatac tgttatttaa 300 agttcaattt tttgtaccta attgctgcac acttcagcaa gaaataacaa ggtatcttta 360 ttaccttcaa cttaagtcac taatacttag tggaaaattg aaatgtaatc taggaattgc 420 tctgagactt gctgctcttg ctgttcaagc tgagttaggt gcaaatgaaa ctacagaaag 480 taaaattcaa gatcttgatc agtttatttt gttaccattt tatttaacac ataaagaaga 540 tgaaaacaga ctacatgagg ag 562 <210> 32 <211> 4911 <212> RNA <213> Hydra magnipapillata <400> 32 atgtctaaag ctttatctgg tcatacaggt attaataacg caacctatca cattcaaagt 60 aattttttct ggtatggaat gataaaagat attacaactt atgtttccaa atgtgaccaa 120 tgccaaaagc caaagaacag aaaacttcag tcgaaaccat tgctgcaaaa tatttcaatt 180 cctaaaggca atatgaagca gatatgtcaa catggctgtt ttgaaattca aataaattgc 240 cagggtcatg agtttgtaaa tgaaatttct aaagaacttt actcaaaaac aggaacaatt 300 caaagaatta cgagtgcata tcattctcaa gctaaaggat taattgatca aatggataat 360 aatgatgact taataattga agttaaaaaa agtgagcaac cttcgcttga tatttatggg 420 tttatgaatg atatcagaat ttcttataat gatgaagata aaaattttga tttaccaaca 480 aataaacctt ctgatgagac tttgacgagc cctaattatg aactcaacaa gttgaatagt 540 ttaacagaac tatcagatag taaaattgac aatagttctt taaatcatga cgatgatata 600 ttagttgaaa agtttaatcc agtccaagat atgaaatatc agaatagcca tttgtctgta 660 ttaccaaatg cagaagatgg ttcagaagtt aaaagtgcta attcttgtaa cacggataaa 720 aattcttctg ataaaggttc ttgtaacacg gataaaaatg agcaagttga gtttaaatgt 780 cttgaaattc cgaaacatag attatcagtc gttaaaaaat cacttattat gcagaataca 840 gcagaaatca gcataatatc tgaatctgaa gttaatgagg gaaaagttaa aaatggaaat 900 actgttgata gtaaaaatga tttagtagaa aatcaatctt ctgaaaaaac cagagcttca 960 tctccattta gaaggagaaa aggtgtacgt ttagcagcag atgttcgaca taatcatcca 1020 gtttttcctg aagatatttt agcagcctac gatatcattg atagaaaaga agtcaaagaa 1080 attaaaccaa ctattgctag taaaagaaat atctcattta ttaatcgttt taaaactgta 1140 ttctcccaca attattcaaa agatcaaaaa attacatcac agcaaaaaag ttctattgtt 1200 cacaataatc ttgtgattaa gttatcttta gaaaaagcaa atgatggtga gcctatgcag 1260 gttataacaa tgtctcattc tgttaagcca gaaaataaca atccaggatc atctatgttt 1320 atttatgttg atgatgagtt taatagtgat gaagaaaatg gcaatggttt acctcgtact 1380 cctagtgatg ctgactctga tcgtagtagc atttttaatg aagatgatga cacagacaat 1440 attgacatgg tagacagcta cagaagttat gctgtaaatc aaaacaatga agtaaatgga 1500 agtgtttata ctgcaaaaaa ggaagatgta ttttacattg aaaaaaaaga ttatattagg 1560 cagggtttgt tatcctattc tccaatgaag aatcagtctg tttatgatgt tgagaaacaa 1620 aataaccgaa gaccaaaaat taatttcaat aaagcatcaa ttgaaacaaa acaaagaaga 1680 ataagtagtt tggtgaaaga aaatgcacca gaagaattat tatttgatgc aagtaaaaaa 1740 tgtaaaaaaa caggaagttt accaggcgca aaaacatttt cagctgatga agtgcttgct 1800 ttagttttag cttgtgggct gactgaaaat gcatatcaac ttgtaagatc tgcagcagca 1860 attaaaggcc acaataacat gtatccagca tataataatg ttaaagaatc taaaaaacga 1920 tgctattcgg aaatagaaac ttgggatgtt gacagttatt cagcatcagt taatatgcag 1980 agtcttataa atcatactgc attaagattg gtctctttac aaagtattgc tttttcacaa 2040 agttgtgtat taagtttaac tttaaggtca gaagttggtt ttgatggtgc aacaggtaag 2100 gcaatataca aacaatttgc gccagatcaa gatgtacgca atcttattat tgaaggaagc 2160 ctacaccaca tatcagagct aaagattgtt cacttttgtg atgttgtgac tcaaagaaag 2220 acatctggaa tagagaatcc tttagttaaa atactcaaag acatatttga aactcttggt 2280 tttcattatg atactaataa cgagccatta cgattgttta ctgatggctt tccagcatca 2340 ttgcctgctt ttccttctga tcgtcgttca ttaattgcat tagaggttta tacaacagaa 2400 agaagttatg taagagggtt ggagattgtt gttttgcatt ttatgaaaca gatcagttta 2460 aatggattca tggatgattc agaccttaaa attgtttttg gtaatattga aagcatctta 2520 gccttgcaca aagaaatgtt ggatgaacta cttgaaagga tttcaaactg gtcagatagt 2580 caatctattg gtgatatttt tttaatgcaa gaaaacaagt ggaaaattta ttctgattat 2640 tgtacaaatt atgatgcaag tgatgctttc ttaaaacaaa gactgaagaa gaagaaggat 2700 tttgaaactt tcttaaatat gtgttacact aatccagtat gtttacctgg cttgacaatg 2760 ccatcatatt taattactgt tatccaacga attccacgat atgttttact gctcaaggat 2820 attgctaaga gaactagtag cgatcacaat gattttcaac acttaaaaga tgcactaaca 2880 cttatggaaa agattgcttc ttttttaaat gaacaactaa aacaatctca atgtcagaaa 2940 gctttagaat tacttcaaac acaaattaca ggacttaagg cttattatac accagatcgt 3000 aatcttgtcc atgagggtgc agtttgtttg cagacaagca aaaagacata tcaatgtgtt 3060 ctattcaatg atctgcttgt ctttgctgtt aaagttgcta ataggcagag cattgttgaa 3120 ttagcattag gtttaaaaac agtatggatt gaagatttgg aagggttgga tccacaaact 3180 aacctaagag aaacttcttt tgaatatgat gatggcagtt tatatagtgg tttctttttg 3240 ggatccaagc gtcacaaaga aggtacaatg atgtggccaa atcaaatgac atataaggga 3300 gaatgggaag atgatgaacg aaatggttat ggaactttag gatatatttc tggtgaattg 3360 tatgaaggtc aatggaaaaa tgacaaacaa ggtcaaattg taactatatt ttatgggttt 3420 gggactttta ttgatgcaaa tggagacagt gttagttgca aatggaaaaa tggtcagcga 3480 cacggagaag caattattaa ttacaaaaat ggtgatatgt ttaatggatt ttttgtgaat 3540 gataatatag aagggattgg tgaattgaag tgtataaatg gtttatgtta taaaggaaag 3600 tggagaagga atcttaagca tggaaaagga aatttaaaat tacctgatgg ctcagaatac 3660 agtggttatt tctatcgaga taaatatcat ggcaagggta aattaactta ttcaaatgga 3720 tcttattaca ctggagattt tgagcgtggt gaaagatgtg gtgaaggttc ctatataata 3780 cctgggggag tttcatatgt tggtcagtgg atgcatgata tgaagaatgg taaaggaaag 3840 atgacatatg aaaatggtga tgaatatgat ggaacatggt atcaagatat gagaataggt 3900 caaggagtat tattctacaa ttatggtggg ttttatagag gacaatggca aaatgatcta 3960 atgcatggca ctggagttat tgagtataaa gatggttcta cttatgacgg tgaatgggat 4020 aataataaga tgtgtggcca tggcaaagta aagtatcctg ggggaacttg ttacacagga 4080 gcatggaaag acaattttcc taatggtaaa ggaattttaa ctaaggccag tggttggtca 4140 tgcgatggga actgggtgca gggcaagtgc tccaaaacac ttgaaatgtt gacagtggca 4200 tacagtgaaa atgttctgag taaaaaaaat gtttacaagt ggtacaaact ctttcaagat 4260 ggccgggaag atgccaatga cgagcttcgc tctggttgcc caatcacgtc aacaactgat 4320 gaaaacgttc aagcagtgaa gaaaattgtt ttggaaaatt gtcgaatcac tatcagagaa 4380 gttgctgagg atgttggtat atcgcttggc tcgtgccatg aaattttttc taacgttttg 4440 gacatgaaac gtgtgtcagc gaactttgtt ccaaaattgc tcaattttta ccaaaagaac 4500 tgtcacatga gcattgctca agaactgttg aatgacgtca accatgaccc agatttactc 4560 aaaagggtca taactggtga cgaattgtgg atatatggtt atgatattga aaccaaagcc 4620 caatcgtccc aatggaagag cccagttatg cgtcgtttgc gagaagcaat acgaagaaaa 4680 tgtccagaat tgtggaaaaa caattcatgg cttttgcatc acgataatgc ccctgctcac 4740 tcatctttgc ttcaagaagg acaatgtaaa ttatgtgatc cctccaataa ctatgagtat 4800 gatggtcaat ggtcaaatgg tttaaaggaa gggaaaggtg ttgaagtaac aaaattttca 4860 acttatgttg gggaatttat tgaagatcta gtatggattc ttgttcttta a 4911 <210> 33 <211> 3160 <212> RNA <213> Hydra magnipapillata <400> 33 ggttatattt acaagcaatt gcctaataaa atggaagaag aaacatattt aaaaaaatta 60 tcatatcttt atggcagttt tattgctgaa atacctgata gcttaaagga attgcacaaa 120 aaaattcctg gtcgtgacaa aagcatgctt aaagaaaatc ttttatctca agatgttgca 180 ctaacattac ttcaggagaa taaactttct atacgacgcg ctgaaatggt gagtattttc 240 agttatttca aagatttacc accaattgaa ccaaaaacac caccagacat tatgtttctc 300 actgtgttgt atcaatcaaa tactattttt catttaattg agaaacattt cacagatttt 360 atagtaagaa ctgtagggtc atcaactata tatggtgagt gtgcactaaa aaagaaacag 420 cttgttgaca accttgagtc taaattaaac agtggaatgg aacgagtttt gtcaagtatg 480 gttaattaca tgaagttttt gttaaattcg gagcagaaaa agtctgactt tcgaccagat 540 ggagaagatg gtgcaatgac ccaaacttct gcatgcatat catgctgccg ctatataaaa 600 acattggtac caagtcttta tgatgcattg gatggaaaaa atctagaagt cgttttaatt 660 gaattaggaa cacgctttca taaagtactt atggaacatt tctttcagtt cacttacaca 720 gatataggtg ccatggttgc actatgtgac gtgaatgaat atagacatgc tattaagtta 780 tttaaggata caaatgaaga agaaaatgaa ggagctcagg aagaaaatct agcctttatc 840 aacccagaat ggtcatcaac tatatatggt gagtgtgcac taaaaaaaaa acagcttgtt 900 gacaaccttg agtctaaatt aaacagtgga atggaacgag ttttgtcaag tatggttaat 960 tacatgaagt ttttgttaaa ttcggagcag aaaaagtctg actttcgacc agatggagaa 1020 gatggtgcaa tgacccaaac ttctgcatgc atatcatgct gccgctatat aaaaacattg 1080 gtaccaagtc tttatgatgc attggatgga aaaaatctag aagttgtttt aattgaatta 1140 ggaacacgct ttcataaagt acttatggaa catttctttc agttcactta cacagatata 1200 gaaatatcga gattagctat acaaaacgtt ataaaattgc atgggagttc tagatgtcaa 1260 agttgccatg gcgtttgcaa aactccaccc cgtgatggtc gatacctaaa aagtattgtg 1320 caagccagtc ctcacacttc atctgcccga gttacccaat tagccacgga aagaggcatt 1380 aatattagtg ccagaagtgt ttgcagaggt ctgtctacag aatttaaact gcatgcgcgt 1440 agaccagcaa agaagccatt tctcaccaag aagcagcttc aggcacatgt gaaattctgt 1500 cgtgatatga aggacaaaac tgctgagtgg tggactactg taatgtttac tgatgaaagt 1560 actttccagc agctttgtgg ttcaggttac aattatgttg atattgataa gaatgttctg 1620 caacaattta ttcaattgcg tgctgatttt aaaagtttaa agattggaaa aaacagtaat 1680 ttaattattt acatgtcttt ttcggtggga attgacattg gaacaacttc aataaaagtg 1740 tgtattctta aaaactgtga tgttatagaa tcattttcag catctcataa tgctacagtc 1800 tcaacatctt ctcgcttatt tcatgagcaa agtccagaac ttattctgca aatgcttgat 1860 cattgcttaa atcaactaaa acatataaaa gaagttgaca aaatttgtat tactggacaa 1920 atgcatggtt tattgttgtg ggagcaaaaa caaagtcaaa atacaagcaa caactctttg 1980 atatccttgt ataaaaacac tgtttttgca aatacaaatc ttataacatg gcaggatcaa 2040 cgttgtgatg aagtattttt aaaaagttta agttgtccaa atatatcttc tggctttggt 2100 cttgcgtcta tgttgtggct gcaaagagaa acaccaaaca tttttaattc gtatcatatg 2160 tgtggaacaa ttatggattt tgtggtttgg ctactcactc taaaaaagaa gccatacatg 2220 agtgttcata atgctaacag ctggggttat tttgatttgt taaaaaactg ttggatgata 2280 aatgagctac agctggactt atttgattgt tcaattcttc ctgatgttgt accagcagga 2340 actattctgg gtaatatatg ctatccagtg tatggaatga gttgttcttc cgtagtttat 2400 gtagcacttg gagatcatca atgttctatt cgcgcatgct cagcacaatt atcagaggca 2460 gttttaaata tggggacatc ttgtcagctt tcaatacgtg ttccttataa tcaagactct 2520 atgacgtcag tattggatat taaatcgaat agtccaacaa tagcaatcac tccttacgat 2580 aagaatttcg ttttgcttac agctgcttct ttaaacggtg gtaacgtttt tgaccaattt 2640 gtttctactt ttttgtcatg gtgtcaaata ataggttgtt caaatatccc atctaacgaa 2700 gaactaatgg acaaactttt atcgatatct ccatttgaag acattcgatt tattgaagat 2760 tctctaatta taactccaac tatttatggt gaaagacacg atcctagtgc gcgagcaagc 2820 atttccaata tatcaccttc aaatttaact ttatctggta ttacgcaagc gttgtttgaa 2880 ggtttatcaa aaaatcttct agctatgctt ccacttaatt gcttgaaaaa gtatggtatt 2940 actcaaattg caggaactgg tcgctgtttt gtacaatgct ctagattgcg agaagctatt 3000 tcaaaagatt ttgaggttcc gtggagatta gtagataaat caagagacgc ttctttagga 3060 gccattttgt tttcactcga catggattaa aaaacatttt taaaatataa tttttattgc 3120 ttcttttttt tttaaatcat aatattttta ttgttaagtt 3160 <210> 34 <211> 813 <212> RNA <213> Hydra magnipapillata <400> 34 tcaaaaaatg tctttgtggc acattcctgt tcacaaatat tttgaaaccg atccattcat 60 ggatgacata atggagataa cttttcctcc tttaagatat tttccattat ttaatgttgg 120 aacaaaggct gtttcaaaaa aaaagactcc gaaagaaaac ggttttgttg taaatcttga 180 tgtcaagcat tacaaaccag aggaagttac tcttaaagta gaaggtcaag ttcttgaagt 240 cagtgggaag catcgtaacg aaaatgaaaa tggattcgag tctagtgagt tccacagaaa 300 gtacactatt cccgatgatg tagatgtgac agcacttacg tcaaacatca gtcaagacgg 360 tgttttgcat atagaagctc ctaaaaagct acctgcaact tctggagaat caacaaaaga 420 gactttcaaa tgcactttag atgtacaagg tttcaagcca gaagaaattt cgattcaagt 480 aaagggtaga gatttagtcg ttcatggtga aacaaaaact gaaaacagcg gtgaacacgg 540 ttcaagtttc caccacaagc agtttacaaa acacgtagca ttgccagatg atgtagatcc 600 atccgaatta agttctcgct atacgaaaga ctctaaatta gcaatcgaag ctccgcgaaa 660 gcaactacaa gctccactca aacttgaaat caaaatggaa gaatgaagtg gtgtcaatcg 720 tttgtagccg tgtttgtagt tatttgtaaa agtttgtgtt tctaagtgtt aatatttacg 780 ctgaatttta tacaatttaa atgctcttca tct 813 <210> 35 <211> 1067 <212> RNA <213> Hydra magnipapillata <400> 35 gtgtctaaag aatagagttt aggtcgcttt tatatgcttt cataaaaaag cgtgtgatct 60 acaattttga acaagcagct ctctttttgc aggtttaaac tgctatgaac gcaatggtaa 120 catcgcaagc taaacctgaa tttatgacgc cacatacaac acaattaggt ctacatcaaa 180 gtcaatctcc agctcccaca gtagctccca cttccccaaa tccattaaat ggagatatgt 240 ctcacgtgaa aaggccgatg aatgcattca tggtatggtc acgtggcaaa agaagacaaa 300 tggcacaaga caacccaagg atgcacaatt cagaaatatc caaacgatta ggagcagaat 360 ggaaatgtct gacacagcaa gaaaagcaac cctttatcga tgaagcaaaa cgccttcgtg 420 ctgttcatat tcaggaacac cccgattaca agtataagcc aaaaagaaga aaacaaaaaa 480 ctactaaaaa agatatatat acgccttacc caaatattgg tcaaggaatg gttcccaata 540 ttgattctaa atatgcttca attggttatc aaccgacact aagctacgga atgagctcag 600 atatgtataa caaattgaac ggaggttacg gttaccagac aacaattagc accggttacc 660 ctttaatgta ttctaattac agcgttgggc caagtatggt aggttctcac tctcaatctt 720 ctcctactgg agcacatcaa tcatacccat cttctacaat aacttcacaa attggtacac 780 ctgtcataac agattccaca taccgtgttt cgacgtcaga ctatattaat agtaaaaatt 840 atttttcaaa catgaacagt ccttatagtc cggtagattc agctgctacc tcaactcatt 900 ctcaaaatcg atatcctact acagacgaaa acagaaatat tgtaaatcac gcacattctg 960 ttggaaacgg aatggtatcg aagttaatac aagaacacga tgttcagtct aattttccag 1020 acaacgctgt aaatagaaac tggtcattaa atttttcttc tgtttag 1067 <210> 36 <211> 755 <212> RNA <213> Hydra magnipapillata <400> 36 aaaaaaaatt gtcaaatgac cattttataa ttaccaaatt ttgattgtca aatgataatt 60 tcggtaactt ttgaactaat atctctgggc aaaatattaa tatttgatag ctttatttat 120 acttaacagt ctgaatgatg gagtttataa tttttagtac tgctttaatt ttaatctctt 180 tgttgattgt ttttttaact tattcgggat ggatgagcac catagatatt caagtaacag 240 aagtttcaac gccaggaaca ttcttttaca agttttatca gcagagttac tctgaatgct 300 ctttggttct tcgtgaattg tttaaatttc ctgttataaa aaacaattct aaacttaaat 360 gcatgggaat ttattatgac gatcctaaaa agatagaaaa caacaagact cgatatgcta 420 ttgggctttt tgtgccatca gatgagatta gtgatgaatt aaaaaatacc atgactggta 480 ttggttattc acatgtccaa ctacctccag ttaaagcaat tgttgctgaa tttccctttc 540 aattttggct ttctattttg gttgcaatct atcgtgttta tccaaaacta aagacatact 600 gcgaaaacca aaaactaaat gcgcatcctt acttggaggt gtacgatcaa aaaaccattt 660 tctttgttgc accactggaa aagaacgaat tattttatgt tgacgaatac aaatattcaa 720 gtattgacta attcaaaata tttatgctaa aaggt 755 <210> 37 <211> 912 <212> RNA <213> Hydra magnipapillata <400> 37 taacagatat gggagatgaa gaggtgcatg cgaagagcat tggcttacga attcaaggca 60 aaatagccag caaaatgtct tcaaaagggt tggcgaaaca agttgttgat ggacctacct 120 ctgaactaat tgataactgt tatagaattg caaaatacta tcttgagagt aaaaaagatg 180 ccgaaaaaat tatgaaaaat atgataaaaa ttgttgtcaa ggttgcactt ttgtcatcta 240 acaatcgttt tacaaaagaa gaaatgtcaa ttattcaaaa tttccaaaac aaatttaaaa 300 ccattgcaaa atctataata agtttttatg aggtagattt tacatttgat tgtgagtata 360 cggttactat gatgaaagat tctcaaaagc ttttagaaaa gttggtttgc aaccatctca 420 ctgcaaagtc attaaagcgt atagagtgtg tttataattt ctactcaaat ccagatgttt 480 tagattctgt tttccaacct ggtagcaagt ttagacctta tctagccaat attgttaagt 540 cattaaacac tttgatagag caaggccagc tataggtttc gctagcttaa aagtatttta 600 aatgcttcgt attttttttt tttttttatt gacataaaat gtttctgaaa tacgtcgatt 660 ttctctcttc gacgtttttg ttttttgttt ttttatatat gcatttttct agctatgcaa 720 tctttacgtt ttcatatttt gttttttgac taaacacttt cttccactta tctttatttg 780 tacattatct cattctcttg tttttgtttt ttttttaatt ttgtctttca cagggaataa 840 ttatagattt tttttttgca atcaacattc gtcagcaaag gaaaacattt tgtgaaacaa 900 aattttaaaa tg 912 <210> 38 <211> 408 <212> RNA <213> Hydra magnipapillata <400> 38 tggaatggtt caaaatataa tcacaaaatt ctaagattaa cagagaattt gaaagaaaat 60 atgaatgaag aacaattatg taaaatattt ggaaagtatg gccctttggc tagtgtaaaa 120 ataatgtggc cgagaacaga agatgaaaaa tcacgtaata gaaattgtgg ttttgttgct 180 tttatggtga ggaaagatgg tgaaaaatgc ttagctgata tagaaggaaa agatgtcatg 240 gattatgaaa tgaaaatagg ttggggcaag tgtgtaccaa ttcctcctat gcctatttat 300 attcatccat ctcattcaac agctgttcga cctcctaaac aatctgggct tccatttaat 360 tgccagttat catattcatt gagaaaaagt ggtgtcgaat ttgatggg 408 <210> 39 <211> 1415 <212> RNA <213> Hydra magnipapillata <400> 39 aaaaaaatct atgaaaaaag accgacattt tcctatgcta ctttttgatt taaaacgttg 60 ggtaaaataa gttcaatcaa atgttgatta aaaccaaata tgaaaactgt tagtttcgtt 120 agttttattt tagttttgta tcttattttt attcggatgg caaaaaacgc acatgcttat 180 tggtggacgg ttggtaaaac taacatgaaa gatacttcag tacaaagtat tttagcaaca 240 cacaagttta gcaaaaatca acgtttacta ttaaagcaat ctccagaact tttattttct 300 attggtaaag gtgctgcaat ggcaattcaa gagtgcaaaa gacagttcat taacagtcgt 360 tggaactgct ccgattatag tccagaatcc gtttttggaa aaattcttca gagagcttgc 420 aaagagacat cttttattta cgctatcact tcagctggtg cgacatatgc attaacagaa 480 ggatgtgcaa aaggagcagt taatggctgt cattgccaaa gtggcttaaa caatcgtgta 540 cgtgagaaac aagattgggt atatgaaggc tgtcatgaca acatccaata tgggtatgaa 600 aatggaaagg cttttacaga tgcaaaagaa actagccgcg attttaaagg gttggttaac 660 ttgcataaca atgaagccgg aagaacgcta gtggtagatt tgatgaaaca agaatgtaaa 720 tgtcttggag tatcaggaaa ctgcaacgtg aaaacatgcc gccgtaaact tagttctttt 780 caagaaattg gaaatcgttt aaaagaactt tttaaccgag ctacaaaagt gcaaccaaat 840 caaatattta gtaaccgaag aataacacgc aactatccaa taacacaaaa tgttgaaaac 900 tcaaaaatta aaaacacata caatatagta tacggagaag aatctccaaa cttttgcaaa 960 tacgatttaa atgtaggctc tttaggaaca ctaaatcgat attgtaacgc taccgtggga 1020 gctattgaag gttgcgagca attgtgctgc ggtcgtaaat ggaaaactga aaaactaact 1080 agatctgaaa gctgtaactg cgtttttaaa tggtgttgca acgtagaatg ccaagagtgt 1140 aaaattacta aagaatacag tttttgcaaa taaccaaata tttatttaaa tttaattttt 1200 aatacaaaaa taaactctca cataaaataa aagtttgtaa ataatttaat ttctacagag 1260 ttgtctaaac aatatttgtt tgttgttttc aaagttattg ttttctgcgt gcacttgtgt 1320 atgtgtgtct gtgtgtgggt gtatctgcag aaataagatt agaaaaatat ttatttcagc 1380 taaataataa tatatatact taaatttcac taaaa 1415 <210> 40 <211> 729 <212> RNA <213> Hydra magnipapillata <400> 40 atgggtacat ctaaaggaat gccaaatttg cgagccattt caccatttaa agagacaaaa 60 gtaggagaag agaaaaatgt taacagtttt gaaaccgcat tagctctttc tcttcatggc 120 tgtcatgtaa tactagcatg tcgtactaaa attaaaggtg agcaagctgc gtctcttatt 180 ctaaaaaaac agaaaatacc aataaaagtt gatgttgttg aatgtgattt ggcttctttg 240 gatagtgtaa aaagatgtgc tgaaactatt ctactaaaaa aatgggctat aaaaattctc 300 atatgcaatg ccggcgtaat gggccttcca tattctctgt catctgacgg aattgaaagt 360 acttttgcca tcaaccatct tggtcatttt tatttggtta atttattaaa agatgtcctt 420 ttaagttcag caccagctag agtgataatt gtgtcatcag aatctcatcg atttccttca 480 ctttatggag acacgtttga gatacgagat gttccaatga aaaaatctga ttatatctca 540 atggtcgcct ataaccaatc aaaactttgc aacctacttt ttgcttttga acttaatagg 600 cgtcttgaat catttggagt tacttgtaat gcagttacac ctggttgttt aatatccacc 660 agtattcagc gccattcata tttttataaa cttttgtttt tgctagcaag gccttttgca 720 aaatcacag 729 <210> 41 <211> 834 <212> RNA <213> Hydra magnipapillata <400> 41 atggagcttc cgaagtttgt aaagcaaggc acattatgtg aagattcttt tatagaaaac 60 gtttttaaaa gaagttgcgt agaaaagttt ccgagagaga aaagtgcgaa acgtccaatg 120 aactcatttt tgctttgggc aaaatctgta aggaaaaatt tttcaaatga taatcctaat 180 ctaaccaaca ctgaaataag tagagtgcta gggaaagtct ggaaagaaat gagtgaagtt 240 gagaaattgc cgtttattca aagtgcaaag tgtcttcgaa ctaaattttt aaatgattat 300 ccaaattatc aatacttgtg caaaaaacgg aaattcaatc aagtaaataa tacaagtttt 360 tccaaaatgg cgtccaaact aggaagtaat gatttaaatg catacgaaat ttttaaatgt 420 ttaaacatga gtgaaattat agaacacaaa gatttgaaaa gctttggcta ccttcaaagt 480 gtattcactg cttatcaaaa cgaaagaaac gacaatcaaa atatattttt gcctgaagtt 540 tttacaaaaa gcataagtaa taaatttgat tccaatgtca aactaaatac taaatcaaat 600 aaaattaaaa gtgacagttt tgatgaaaat caacaaatgc aaaaagaaaa aactacgctg 660 cattactatt cagatgaaac acaacatttt aatcaaaatt cctttgacat aaatagcgaa 720 gaagatttta atcaaaataa tcaaataaaa gaaagagaag atgttattga acttgactct 780 gaattaagag aattttttca gtctcttgag aaaggttttg gttattatga atga 834 <210> 42 <211> 491 <212> RNA <213> Hydra magnipapillata <400> 42 tcattgtagt aatgtctgac gctccgaaaa caggacgaaa gcaggcgccc aaggtggcca 60 aaaagggtga aaaacgagct ggcaaaaaag gcggcaagat cgccggtaca ggagataaga 120 aacgcaagaa gaagagacgc gagtcctacg ccatttacat ctataacgtg ttaaagcaag 180 tgcatcccga tgtcggagtt tcgtctaaag cgatgagcat catgaattcg ttcgtgaacg 240 acatttttga aaggatcgcg tctgaagctt cgcgactcgc ccttcaaaac aaaaagtcta 300 caatctcttc acgtgaaatt caaacagccg tgcgcctctt acttcccggt gaattggcaa 360 aacacgcagt gagcgaaggc accaaagctg tcaccaagta cacgagcagc aagtagacgc 420 tctactacaa aaaggctatt ttcatagcca caaatcttta taaaaacggc gttgtgcgca 480 ttagaacaag a 491 <210> 43 <211> 803 <212> RNA <213> Hydra magnipapillata <400> 43 gttggaacaa agcttacaca gtagctatca aatcgtttac caatggaaaa ttcgtatgtg 60 ctgaaaatat cggaaatcaa ccattaattg ccaatagaga tgctattggt ttatgggaaa 120 cttttgaaat tcgtttcact aacgcacaaa catttgcatt aaaatcacat gcaaatggta 180 agtttgtcag tgcaggtaat ggtggaaata acttgctcat tgcaaataaa gaccacgctg 240 cagtatggga gactttcacc ttagttccaa aatatggagc ctttggattt aaatcaaatg 300 gtaatgcaaa gttagtgact gctgaagaag caggaaacaa accacttgca gctaatcgca 360 ttgttcttga cgtctgggaa atgttcagct tagtctacgt ctggccatca gtacacaaag 420 tggcaataaa agcattggtt aatggtttat ttgtatgtgc tgaaaatgcc ggaaagcaat 480 cattaattgc taacagaggc caaatagggc cttgggaaac atttgaaatt cgcttcacta 540 acccccaaac atttactctc aaatctcttg caaacggcaa attggtttgc gcagaaaata 600 atggaaaatc tcctcttatt gctaatagag aacttattgg accatgggag acctttacgc 660 tggttcaaaa taaagaagga tttgctttca aatcacacgc aaatggaaaa ttagtgactg 720 ctgaaaacgc aggtcatagt aacctaattg ccaaccgcga taatcctgat atttgggaaa 780 gattcgtttt tatttacttg tga 803 <210> 44 <211> 2013 <212> RNA <213> Hydra magnipapillata <400> 44 atgaaaagtt ctagagtaag tcctatattt tccgtaacac ttaatcaagt tgatcaacca 60 attcaggttg atcaaccaac tcaggttgat ggtaaaaaag atgataaata tcaatctgga 120 aatgagaaca taatttacga aaatgaagtt gcaaatttta actcgctaca aaaatatttt 180 tcgttaaaac acacccaacc acctatgcca ctaatttccg ttgcttttaa tgaaccatgc 240 aaaaataatg tagacaaagg tataatacca gatataaacg atgagacgtt aataaaatac 300 ttctctgatc acaacaaaaa ggataaagac cacaaaatat tgatcgacca tcattattta 360 aacgaactac tcttatctaa cataagcatc aataatacag accgatatgg acagagtgtg 420 atgcatgagg tggctagagc ttgggatact gaagttgcaa aacttctatt aaaacatgtt 480 ctagctgcag agcttgatag gtcatttgca gcaaaatatt taattgaaaa tggagcagat 540 gctagtgtta aagatagtag taacatggct gtgatggctt tacttgcgct cgaccagttc 600 cacagcatag atcgcaaatc acgcacagag cactttaact taaattgtct tactttacca 660 ataaacgaga catcgtgttt tgcaaagtca cctttagaga tcacagtgga aatgaaacaa 720 tacgaaattt tattacaccc ggcaatgaat gaactaataa aagttaaatg ggcaaagttt 780 ggaaaatgta gtgctctaaa aagtattgct gctaattttg cacttgtaat attttggaca 840 ttactaatgt gtacaatgcc acatgaaaaa agttcaatgt atacaggtaa acgagaatat 900 tggggaccta ttgtcgagat tatagcagta gacacattag caataaatat tttattggaa 960 gtaaaagatt tctataaatc tctaaccagg tttaaaaagt ataaaaaatg gcgagaaaaa 1020 gaaattcgta aagatttaaa atattgtcat aaaaaatggc cagaagaacg tacttactta 1080 aaacaagaga tacgcgagct caaaaactcc aagctatcat atataaaaga ttattggaac 1140 atttttgact gggttactta ttttttgatg gcattcagta tatctttaca tttaattgaa 1200 atggcgagac aaaacaagta ttcattgaca acaaaaaaat attcagcctc agctctaatg 1260 gtgtgcatgt ggcttcgcct tcttaagttt gcacgtccat tcaaactact tggtgttttt 1320 gtggtaatgt taggatatat tgtaaacgac gcactaacga tactatatct agcgatgcat 1380 attttcattc cttacacagc tctattttat ataaattttg gaatgtacaa tattgcagat 1440 tactcagttg ggaagctaca actattttat aatatttttc aaatggcgct aatgggtagc 1500 tatagttacg atggaattgc taatcaagat acagtattag cagaaatatt aaccgctact 1560 tttatttttt ttgctgtagt tataatattg aacatactta tagctatgat gtccaatact 1620 tttcaacgcg tgtatgataa tgctaaagca aatgctttaa tgctacgagc tgcatttata 1680 ataaacgaag aaattaatat gtcccgcagt cagcttattc aacattatga gtggattaat 1740 gcaaaatgtt cgcctcaaac tgtttattac aacgaatcga aagtttattc tcgcgaaaat 1800 aaacttttgt ctcaagaaaa tgattttata aaagcgacaa acgaaataaa caagaaaatt 1860 gataaagtga taaacacaat ggaaaaactt caatttcaga ttaaaaactc aatgctggaa 1920 agtcaatctt atgtgaaatc tgaagcagtt gagccacaat tgaaaagcct aaagtggata 1980 aataataaga cagacaatga attccaaata tga 2013 <210> 45 <211> 1397 <212> RNA <213> Hydra magnipapillata <400> 45 atacctgtat aaaagccaac tatgttgctg attttacaat agactgtttg tctccatata 60 aaacatattt agaagaaaca agagattttg atttaaattg gacaatacca aaaaattata 120 actcacctat ttatccttca actatgccat ggagatatca aacatggcaa gaacttgatg 180 ggtatccata tacagcaaat ttagacacct actatggtgg aggttatgtt atagaaatct 240 ttcctaaatg gaaaaacaag gctatactgg aacaagtaaa aaaatcaaga tggattgata 300 gacaaacacg agctgtaata atagagtttg ctttgtttaa tgctgctacc aattatttta 360 gcatggtcac aatggccttg gaatttcctg cctctggtgg agttatccct aatttttctg 420 ttctaacatt taagttgtat gcatcagtaa caggttcaaa agttatgtta ggttctcatt 480 tactttttat tttaataaca attttatttg ccattcgaga atgtcgttat ctttatcaaa 540 ctggtcctaa atattttctt gagttttgga acttggttga agttgcattg ataaatttat 600 caattattgc ggttgtgttt ttcttttaca aagggtattt agccaagatg ttgttacaac 660 ggattccgca aaagaaacca aataagttta tcaacttcca gtttgcttct tactgggatc 720 tgacatatat atacattgta tcactcattg tattttttgt aactttaaag ttcattaaac 780 ttttgcaatt caatcgtcga atgttgatgt tatcttctac tttaaaaaat gcatggtatc 840 ctttgactat gtttggaata atatttttca ttatattgtg ttcagttgtg tcaacagcta 900 gcattgtttt tggttcattg ctgtatggat atcaaaatta cttcaaaaca ttagcttcta 960 taatttcatt attgttggga aaatttagtt acagtcagtt tgaaaatgca aataatgttc 1020 ttgggccgag cttttttttt ggatttaatt tcattgttat atggattgtt atgaacattt 1080 ttatttcaat attaaatgat gcttttgctg aagtttttgc agatctccag tttcagacta 1140 atgaatatga aatggttaat tttataattg ttcatttaaa agaattgttt ggatggagcc 1200 cttctaagaa aagtgtggat gtaactacaa atcaaaaagt aggacttagt caggatagca 1260 gtatggatta tatgggattg cttagaaagt caaaattaca tcagttcaag gaacttaacc 1320 aagcgaataa aacacattgt ataactcttt aaaaaaaacc aatcattaat caaattttaa 1380 aactgtcaaa ttacgaa 1397 <210> 46 <211> 834 <212> RNA <213> Hydra magnipapillata <400> 46 atggaacttc caagatttgt aaaacaaggc acattatgtg aagattcttt tatggataat 60 ctttttaaaa gaagatgcgc agaaaaggtt ccaagagaga aaacagcgaa acgtccaatg 120 aactcatttt tgctttgggc aaaatctgta aggaaaatgt atgcaagtga aaaccctaat 180 ctaagcaaca ctgaaataag cagactgtta ggcaaagttt ggaaagaaat gagtgaagtt 240 gagaaattgc cgtttattca aagtgcaaag tgtcttcgaa ctaagtttat acaagataat 300 ccaaattatc attacttctt taaaaaaagg aaaatcaatc aactaaataa tacaagtttt 360 tctaaaatgg cttccaaatt aacaagtaat gatttaaatg catacaaaat ttttaaatat 420 ttaaacatga gtgaaattat agaacacaaa gatttgaaaa gctttggcta ccttcaaagc 480 gtattcgcag cttatcagaa cgaaaggaac ggcaatcaaa atacattttc gcctaaaatt 540 ctttcaaaaa gcattagtaa taacttcatt tcgaatgttc aattaaatgc taaatcaaat 600 aaaaataaaa gtgacagatt tgatgaaaat caacaaacgc acaaagaaac aactacgctg 660 cattactatt cagatgaagc acaacatagc tgtgaaaact ctttcaacat caataacgaa 720 gaagatttta atcaaaataa tcaaataaaa aaaagagaag atgttattga acttgactct 780 gaacttagag aattttttat gtctcttgaa aaaggctttg attatgatga atga 834 <210> 47 <211> 834 <212> RNA <213> Hydra magnipapillata <400> 47 atggagtttc cgaagtttgt aaagaaaggc acattatgtg aagattcttt tatagaaaac 60 gcttttaaaa gaagttgcgt agaaaagttt ccgagagaga aaactgcaaa acgtccaatg 120 aactcatttt tgctttgggc aaaatctgta aggaaaaatt tttcaaatga taatcctaat 180 ctaaccaaca ctgaaataag tagagtgcta gggaaagtct ggaaagaaat gagtgaagtt 240 gagaaattgc cgtttattca aagtgcaaag tgtcttcgaa ctaaattttt aaatgattat 300 ccaaattatc aatacttgtg caaaaaacgg aaattcaatc aagtaaataa tacaagtttt 360 tccaaaatgg cgtccaaact aggaagtaat gatttaaatg catacgaaat ttttaaatgt 420 ttaaacatga gtgaaattat agaacacaaa gatttgaaaa gctttggcta ccttcaaagt 480 gtattcactg cttatcaaaa cgaaagaaac gacaatcaaa atatattttt gcctgaagtt 540 tttacaaaaa gcataagtaa taaatttgat tccaatgtca aactaaatac taaatcaaat 600 aaaattaaaa gtgacagttt tgatgaaaat caacaaatgc aaaaagaaaa aactacgctg 660 cattactatt cagatgaaac acaacatttt aatcaaaatt cttttgacat aaatagcgaa 720 gaagatttta atcaaaataa tcaaataaaa gaaagagaag atgttattga aattgactct 780 gaattaagag aattttttca gtctcttgag aaaggttttg gttattatga atga 834 <210> 48 <211> 1383 <212> RNA <213> Hydra magnipapillata <400> 48 atttgattcg taacacgcca gtaatcttgc tctctatata aagaagcgta ttttgataaa 60 cctatgcaaa tttaataaag catctgcaga gagaaaacga acatcgtgat ataaataaca 120 gataataaaa tgaaagtgat ctttaatgtt gttttactat tcgtggttgc acatggttta 180 gataagctga ttagagatta cgaaacatgt ttaaaaacag cggagttagt agccaccatt 240 cctgtattag ctatgtccta caaggtatcg tttgatttaa aacccaagtc atactcatat 300 ggttttcata gtgttcttca tttcgcagtt ggtaatgata ccagtgaaat cggtgacaga 360 gctctggcac tttggttacg accctatgag cctaactata agggttttca cattactgca 420 ccgataaatg gaaaaagaaa tagagaaatc tatatcgatg cacctcccct caatgtgtgg 480 acacacgttg ttattagtca acaacgtatt gatgacaaat atgttttcac aattgacgta 540 aatggaacaa acgcattttc tgaaaataat gctaatcccc aaaaatttga aaacgtcaaa 600 gtttttgctt cagatccgtg gtatccatct caagatggat cgataaaaaa tcttattctt 660 gaaaacgggg agcctggtga atctttagcg aaagctgtac tgaaatcaaa atattttgta 720 gataatgaag ttgaagagac actgaaaaaa aataacttgg ttgcggtttt gtcaaaatta 780 gaacaatcat ttgatattta tttcgattta aaactcaaca gtttttcaaa cggatttaga 840 agtgtcattc atttgactaa aggagaggat aattcgaaat acggtgatcg aattcctggc 900 atttggattt ttgatcgaat gttacacctt ggttttgcaa taaacaatga aaaaaatgaa 960 gttattaagt ccaagccact tcaacaaggt cagtggataa atattcatat acgtcaacac 1020 gctgaatcgg gaagagcttc ttttacttta tatatcaata acgaaaatgt atatactgta 1080 caaaacttca acccacaagt atttacaaat gtcaaagttt acgcaggaga tccgtggtat 1140 gaagctcagg atggctccat taaaatgttc aatgtcatta atggatattt agatgggaaa 1200 aaggaagtga aaaaggacgc catacgaaaa aaggcgtttc ggttaaatta ttaatatatg 1260 tcaacgactt gattttttat ttcaaatttc tttttatttg tagttttaat agttttttta 1320 gtatgtaatt tttgatgttt tgaatttgta ttttttattt taaaatacat ctttcgcatt 1380 gca 1383 <210> 49 <211> 694 <212> RNA <213> Hydra magnipapillata <400> 49 ttttgaaaca ttgtgtgagt cgcagctatc gttagcaaag tattttcaag aaaaggatta 60 taaatcactt gctaatttgt attatgaaaa ttcataccaa tcaagttgta gaattaaaag 120 tgataacaga aagaaggaag cagaaacaat ttattattta ggtaagttta aagagcttga 180 acacgattat tatatggcac aaaagtattt tgagcagctt aagtccataa ctaaagggtg 240 tgattggtat caagaaggtg ttctgttttt taaacttgct tgtgaatgtc tccaaagaat 300 ttatataaaa agcgtgcata agttggatca tgatacagag aaaatgaaaa ttcttgatat 360 tttgaacatg gcttatttaa ctgctaaaga aggtggtagt tctgaatctc ttcgtgaagt 420 tatttatgtt actggtgaag ttcattctag ctttggtgag ttaaaggatg ctattcgata 480 ttacaatgaa tactttgaaa taagcaaaat taatggagat aataaaggaa ttcaaaaagc 540 ttatcaagca cttgcaagag cttatcagag tgctgctaat atagaaaaag ctttagagtt 600 tttcgaaagg ttgctagaag aagcagaaag gtctcatgac gattgctgcg tttttaatac 660 ttgccaagaa attgcagctc tttataattc agag 694 <210> 50 <211> 560 <212> RNA <213> Hydra magnipapillata <400> 50 atgtacaggg tgcggatagg aggaatcaag tcaaagacaa aggatgataa tgaagacaac 60 gacaaagaag aattagaaaa ctacgaagat aatgatcact cacaaggcta tattaaaact 120 aaatggttaa aacgaactgc acgagcatgc gaaggaaaca atctgataat caattgctac 180 ggtcaaagaa aaattaaagt aatttatgca aactatggta gaacctcgtc tcgtatatgc 240 tctgggaatt ttaatacgga tttgccaaag aaatgtaaca atcaaaaaag atctctgaaa 300 gaagtgcgta acaagtgttc aggcaggtca tcttgcgttg ttcaagcatc gaatggagta 360 tttggcgacc catgttttgg aacgtataaa tatcttgaag tgcgttttta ttgtcaaaag 420 aaacaccatt ttattggtat ttaaatcaat taaaaattgt tattgacagt gtagaaagaa 480 aatttaatta attcatgagt tattttactt atatatacat gattttataa tttttttaaa 540 aacaaatatt atcactagta 560 <210> 51 <211> 1246 <212> RNA <213> Hydra magnipapillata <400> 51 gcgaaccatt gcgaagcaga tagcagaaaa aaaatggtat taaaagattc aaatttttat 60 aaagtgatac ttggtgttgt tctgatgttt gtggttacac atggtttaga tcagctaatt 120 aggggttacg aaaaaagtgt acaacgaaat gaattagtag ctgctattcc tgtattggct 180 aagtcataca aaatatcgtt tgatttaaaa cccaagtcgt actcatatgg tcttcataat 240 gttattcaat tcaccgttgg tcattatatg agtaaatata gaaatagcac tccggcactt 300 tcgtttcaag aggataaaca taaccgtaca gaatttcaga ttgttacatc tataaatgaa 360 aatcctaaca gacaagtata tatcgatgaa cttccactca atgaatggac caaagttgtt 420 attagtcaac aacgtataaa aaataacaaa tatgttttca caattgatct aaatggaaca 480 aatgtactta atgaagagaa caataaaccc caaaaatttt acaatgtcaa agtttttgtt 540 tcagctcctt tgcatctatc tcataatggg ttaatacgaa atctaaatct tgaaaatggg 600 gaaccaggtc aatcattaac gaatgctcta gagacaagag acaatgtgga taataatcac 660 gtagtgaaac tgaagaaaaa taacttggtt gcgagtttgt caattttaga aaaatcattt 720 gatattagtt tcgacttaaa acttaacagc ttttcagatg gatatagaag tgtcattcat 780 ttgacaatgg gagaagatga attaatatac ggtgatcgag ttcctggcgt ttggattttt 840 aatgaaaagt tacacgttgt ttttatacct catcataatt ttaattctga gccacttcca 900 ctaaacaagt ggataaatgt tcaaataaat caaactgttg aatcatggag atctacttta 960 aaaatatata ttaatcacga aaaagtatat gaaaaactaa accctgtggc acgaatcttt 1020 gaaaatatag acgtatacgc tggagatcca tggtatgacg cccaggatgg cttcatcaaa 1080 aacttcgaag tctggaatca cgttcaggct gtcgaatcga aaaaaagttt gaaaaaggac 1140 gccaaacaga aaaaggcgtt tggaaacaaa ctttaaattg ttacaacgac tttatttctg 1200 tttcgaatat gtatttttta tttaaaaata tatctttcaa atcgta 1246

Claims (13)

  1. 서열번호 1 내지 51로 기재되는 모든 유전자 각각의 핵산 서열의 올리고뉴클레오티드 전부 또는 그의 상보가닥 분자가 집적된, 시료내 디클로페낙(Diclofenac) 노출 여부 검출용 마이크로어레이 칩(microarray chip).
  2. 제 1항에 있어서, 상기 유전자는 히드라(Hydra magnipapillata)로부터 유래된 것을 특징으로 하는 디클로페낙 노출 여부 검출용 마이크로어레이 칩.
  3. 제 1항에 있어서, 서열번호 1 내지 40으로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 디클로페낙 노출에 대응하여 발현이 증가하는 것을 특징으로 하는 디클로페낙 노출 여부 검출용 마이크로어레이 칩.
  4. 제 1항에 있어서, 서열번호 41 내지 51로 기재되는 유전자로 구성된 군으로부터 선택되는 유전자는 디클로페낙 노출에 대응하여 발현이 감소하는 것을 특징으로 하는 디클로페낙 노출 여부 검출용 마이크로어레이 칩.
  5. 1) 피검 시료에 노출된 실험군의 히드라와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
    2) 단계 1)의 실험군 및 대조군의 RNA로부터 cDNA를 합성하면서 실험군과 대조군을 각기 다른 형광물질로 표지하는 단계;
    3) 단계 2)의 각기 다른 형광물질로 표지된 cDNA를 제 1항의 마이크로어레이 칩과 혼성화시키는 단계;
    4) 반응한 마이크로어레이 칩을 분석하는 단계; 및
    5) 분석한 데이터에서 제 1항의 마이크로어레이 칩에 집적된 유전자 발현 정도를 대조군과 비교하여 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법.
  6. 제 5항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 디클로페낙 노출 여부 검출 방법.
  7. 제 5항에 있어서, 상기 단계 2)의 형광물질은 Cy3, Cy5, FITC(poly L-lysine-fluorescein isothiocyanate), RITC(rhodamine-B-isothiocyanate) 및 로다민(rhodamine)으로 이루어진 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 디클로페낙 노출 여부 검출 방법.
  8. 1) 피검시료에 노출된 실험군의 히드라(Hydra magnipapillata)와, 정상 대조군의 히드라에서 각각 RNA를 분리하는 단계;
    2) 단계 1)의 RNA를, 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 사용하여 정량 실시간 RT-PCR(Quantitative real-time reverse transcript polymerase chain reaction, qRT-PCR)을 수행하는 단계; 및
    3) 단계 2)의 유전자 산물을 대조군과 비교하여 발현 정도를 확인하는 단계를 포함하는, 시료내 디클로페낙(Diclofenac) 노출 여부 검출 방법.
  9. 제 8항에 있어서, 상기 시료는 생체시료, 식품시료, 화학시료, 공업시료, 임상시료 및 환경시료로 구성된 군으로부터 선택되는 어느 하나인 것을 특징으로 하는 디클로페낙 노출 여부 검출 방법.
  10. 제 1항의 마이크로어레이 칩을 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트.
  11. 제 10항에 있어서, 스트렙타비딘-알칼리 탈인화효소 접합물질(strepavidin -like phosphatease conjugate), 화학형광물질(chemiflurorensce) 및 화학발광물질(chemiluminescent)로 이루어진 형광물질군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 디클로페낙 노출 여부 검출용 키트.
  12. 제 10항에 있어서, 혼성화에 사용되는 완충용액, RNA로부터 cDNA(complementary DNA)를 합성하기 위한 역전사효소, dNTPs(deoxynucleotide triphosphates) 및 rNTPs(ribonucleotide triPhosphates, 사전 혼합형 또는 분리 공급형), 표식시약, 및 세척 완충용액으로 이루어진 반응 시약군으로부터 선택되는 어느 하나를 추가적으로 포함하는 것을 특징으로 하는 디클로페낙 노출 여부 검출용 키트.
  13. 서열번호 1 내지 51로 기재되는 각각의 유전자에 상보적이고 유전자를 증폭할 수 있는 프라이머 쌍을 모두 포함하는 디클로페낙(Diclofenac) 노출 여부 검출용 키트.
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KR101871647B1 (ko) * 2018-01-08 2018-06-26 한국해양과학기술원 플루옥세틴 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법
KR101879998B1 (ko) * 2017-09-28 2018-07-18 한국해양과학기술원 산화아연 나노입자 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법
KR20200125000A (ko) * 2019-04-25 2020-11-04 경희대학교 산학협력단 히드라 단백질 또는 히드라에서 추출한 펩타이드를 포함하는 나노 물질에 의한 생물 독성 저감 방법

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101879998B1 (ko) * 2017-09-28 2018-07-18 한국해양과학기술원 산화아연 나노입자 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법
KR101871647B1 (ko) * 2018-01-08 2018-06-26 한국해양과학기술원 플루옥세틴 노출에 대응하는 히드라 유전자 및 이를 이용한 수생태계 환경오염 진단 방법
KR20200125000A (ko) * 2019-04-25 2020-11-04 경희대학교 산학협력단 히드라 단백질 또는 히드라에서 추출한 펩타이드를 포함하는 나노 물질에 의한 생물 독성 저감 방법
KR102252798B1 (ko) 2019-04-25 2021-05-17 경희대학교 산학협력단 히드라 단백질 또는 히드라에서 추출한 펩타이드를 포함하는 나노 물질에 의한 생물 독성 저감 방법

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