KR101747699B1 - Cosmetic composition containing gap junction blockers for soothing skin and reducing skin irritation - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an intercellular gating agent, and more particularly, to a cosmetic composition containing an intercellular gating agent, such as oleic acid, glycyrrhetinic acid or oleamide, The present invention relates to a cosmetic composition which is excellent in skin soothing or anti-irritation effect.

Description

[0001] The present invention relates to a cosmetic composition containing gap junction blockers for soothing skin and reducing skin irritation,

The present invention relates to a cosmetic composition containing an intercellular gating agent, and more particularly, to a cosmetic composition containing an intercellular gating agent, such as oleic acid, glycyrrhetinic acid or oleamide, The present invention relates to a cosmetic composition which is excellent in skin soothing or anti-irritation effect.

Skin epithelial cells, including keratinocytes, constitute the outermost part of the human body and express various receptive proteins and neurotransmitters, including TRP channels, as in neurons, and are thought to play the forefront of the sensory system. Physical stimulation applied to the dead skin cells causes the inlet (Ca ^{2 +} influx) of calcium ions this intercellular gap junction (Gap junction) and transmission (Calcium wave) to the surrounding keratinocytes via finally passes a signal to the neuron .

Oleic acid belongs to a single unsaturated fatty acid having one double bond between carbon atoms. It is a fatty acid that is also contained in vegetable oils such as olive oil and canola oil, and in animal fats. Its known effects are low serum cholesterol, low HDL cholesterol, good for hyperlipidemia, and contained in breast milk, helping the baby grow and develop. Glycyrrhizic acid is a triterpenoid containing five ring structures. It is derived from a plant called liquorice and is used to mask the bitter taste of drugs such as aloe or quinine. Oleamide is an amide form of oleic acid, a fatty acid, which is naturally present in the animal body and accumulates in cerebrospinal fluid and is known to have an effect of inducing sleep.

These three kinds of substances act as different mechanisms and have the effect of blocking intercellular gap junction in common. In the case of glycyrrhetinic acid, anti-inflammatory and soothing effects are known to some extent in atopy and the like. However, the effect of treating oleic acid and oleamide to skin to alleviate the size of skin irritation reaction has not been studied, Is not known.

Accordingly, the present invention has been accomplished based on the discovery that the combination of oleic acid, glycyrrhetinic acid, and oleamide, an intercellular space-blocking agent, can alleviate the size of stimulation transmitted to the skin.

Accordingly, an object of the present invention is to prepare a cosmetic composition which contains an intercellular clearing-junction blocking substance to calm the skin and alleviate irritation.

In order to achieve the above object, the present invention provides a cosmetic composition for skin soothing and skin irritation which contains at least two kinds selected from the group consisting of oleic acid, glycyrrhetinic acid and oleamide.

The cosmetic composition prepared according to the present invention contains an intercellular intercalation blocking substance, and this intercellular intercalation blocking substance acts on the signal transduction mechanism between the keratinocytes of the skin, thereby causing skin-induced stimulation (erythema) Relieves and calms the skin.

The present invention provides a cosmetic composition for skin soothing and skin irritation which contains at least two selected from the group consisting of oleic acid, glycyrrhetinic acid and oleamide.

Hereinafter, the present invention will be described in detail.

Oleic acid, which is used as an active ingredient in the present invention, is known as a component having an effect on blood pressure in olive oil. In cosmetics, it is a perfume composition, a surfactant, and a material used for cleansing purposes. Oleic acid acts on lipid-protein interactions in intercellular gap junctions to block intercellular junctions.

Glycyrrhetinic acid, which is used as an active ingredient in the present invention, is known to have an anti-inflammatory effect by its biological effect and is mainly used as a conditioning agent in cosmetics. Glycyrrhetinic acid acts on the phosphorylation of connexin 43 protein to block intercellular junctions.

Oleamide used as an active ingredient in the present invention is an amide form of oleic acid and is known to induce sleeping in cerebrospinal fluid of animals. Its derivative is used as a surfactant and viscosity modifier. Oleamide blocks intercellular junctions through mechanisms that induce conformational changes in connexon.

The use of the oleic acid, glycyrrhetinic acid, or oleamide of the present invention alone or in combination of two or more of them, the synergistic effect of the stimulation reaction (erythema) induced in the skin due to their synergistic action, .

The cosmetic composition of the present invention contains oleic acid, glycyrrhetinic acid and oleamide in an amount of 0.01 to 5% by weight based on the total weight of the composition. If it is less than 0.01% by weight, the effect is insignificant. If it exceeds 5% by weight, it may act as an irritant due to side effects.

The skin-soothing cosmetic composition of the present invention can be used variously for cosmetics for skin whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and cleansing, cleansing agents, soaps, and essences. The whitening cosmetic composition to which the skin whitening cosmetic composition of the present invention is added may take the form of a solution, an emulsion, a viscous mixture or the like.

That is, the skin-soothing cosmetic material of the present invention is not particularly limited in its formulation, and examples thereof include softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, eye essence, essence, cleansing cream, , Cleansing foam, cleansing water, pack, powder, makeup base, foundation, body lotion, body cream, body oil, body essence, body cleanser, serum, soap, lotion, lotion, ointment, gel, cream, patch or spray Can be formulated as cosmetics.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

[Examples 1 to 4 and Comparative Examples 1 to 3]

A cosmetic composition having the composition shown in Table 1 below was prepared by the following method (unit: wt%).

First, components 1 to 6 of the raw materials shown in Table 1 were mixed and dissolved at 70 ° C to prepare an aqueous phase. Components 7 to 13 were mixed and dissolved at 70 ° C to prepare an oil phase (oil phase). Then, the oil phase was added to the water phase, followed by primary emulsification with a homomixer (Tokushu Kika, Japan), followed by addition of component No. 14 to increase the oil phase. After removal of air bubbles, components 15 to 17 were added and mixed. Thereafter, the mixture was cooled to room temperature to prepare the cosmetic compositions of Examples 1 to 4 and Comparative Examples 1 to 3.

ingredient Example
One Example
2 Example
3 Example
4 Comparative Example
One Comparative Example
2 Comparative Example
3 contrast
sample Number
Prize 1. Purified water Balance Balance Balance Balance Balance Balance Balance Balance 2. Glycerin 8 8 8 8 8 8 8 8 3. Butylene glycol 4 4 4 4 4 4 4 4 4. Hyaluronic acid extract 5 5 5 5 5 5 5 5 5. Beta Glucan 7 7 7 7 7 7 7 7 6. Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 U
Prize 7. Capricious Capriccio
Triglycerides 8 8 8 8 8 8 8 8 8. Hydrogenated
Polydensen 5 5 5 5 5 5 5 5 9. Cetearyl glucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 10. Sorbitan stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 11. Cetearyl alcohol One One One One One One One One 12. Preservatives Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 13. Incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 14. Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 15. Oleic acid 0.1 0.1 0.1 - 0.1 - - - 16. Glycyrrhetinic acid 0.1 0.1 - 0.1 - 0.1 - - 17. Oleamide 0.1 - 0.1 0.1 - - 0.1 -

[Test Example 1] Effect of skin irritation mitigation on physical stimulation

The following experiments were conducted to examine the skin irritation mitigating effect of the cosmetic compositions prepared in Examples 1 to 4 and Comparative Examples 1 to 3.

Thirty five healthy men aged between 30 and 30 were exposed to skin irritation through a tape stripping method and evaluated the effect of cosmetics according to the present invention. Tissues were applied 20 times or 30 times (adjusted according to the volunteer's skin sensitivity) to the inner part of the left and right underarms with general Scotch tape to cause skin erythema. After erythema induction, control samples were applied to the left lower arm in Examples 1 to 4 and Comparative Examples 1 and 3, and the right lower arm in the left lower arm. The degree of skin irritation 2 hours after application was evaluated according to the criteria shown in Table 2 below. The determination of the stimulation relaxation effect was calculated by averaging the three measured values per each experimental group and using the following equations (1) and (2) for the change of the mean value immediately after stimulation and after 2 hours. The results are shown in Table 3 below.

score Degree of irritation (erythema) 0 No reaction One Very mild erythema 2 Faint or mild erythema 3 There is a clear borderline but weak erythema 4 Pronounced erythema 5 Severe erythema

Evaluation items Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Example contrast Example contrast Example contrast Example contrast Example contrast Example contrast Example contrast Degree of stimulation
(Immediately)
(by subject) 4 4 3 3 3 3 4 4 4 4 3 3 4 4 3 3 2 2 3 3 3 3 2 2 4 4 3 3 4 4 4 4 4 4 3 3 3 3 4 4 3 3 3 3 4 4 3 3 4 4 3 3 2 2 2 2 3 3 4 4 4 4 4 4 4 4 3 3 3 3 Degree of stimulation
(After 2 hours)
(by subject) 2 3 2 2 One 2 2 3 2 3 2 2 3 3 One 2 One One One 2 One 2 One One 2 2 2 2 One 3 2 3 2 3 One 2 One One 3 3 One One One 2 2 3 One 2 One 2 One One One One One One 0 One One 3 2 2 2 3 2 2 One One 2 2 Degree of stimulation
(Immediately)
(Average) 3.4 3.4 3.4 3.4 3.4 3.4 3.6 3.6 3.2 3.2 3.2 3.2 3.0 3.0 Degree of stimulation
(After 2 hours)
(Average) 1.0 2.2 1.6 2.4 1.4 2.2 1.4 2.4 1.4 1.6 1.8 1.8 1.8 1.8 Stimulus relaxation (%) 48.0 24.0 36.0 20.0 40.0 24.0 44.0 24.0 36.0 32.0 28.0 28.0 24.0 24.0 Degree of stimulation relaxation
(Examples / Control Example) 2.00 1.80 1.67 1.83 1.13 1.00 1.00

The results of Table 3 show that Examples 1 to 4 containing two or more kinds of intercellular intercalation blocking substances are superior in skin irritation mitigating effect to physical stimulation as compared with the control sample, whereas Comparative Example 1 ~ 3 were not significantly different from the control samples. In addition, comparing Example 1 with Examples 2 to 4, it was found that the use of all three types of intercellular space-blocking substances increased the effect.

[Test Example 2] Skin soothing effect on irritant substances

Thirteen healthy men in their 20s and 30s who had no history of skin irritation and allergic reactions were tested for skin sedation by human skin patch test.

5% of lactic acid, which is a skin irritant, was added to the cosmetic preparations and control samples of Experimental Examples 1 to 4 and Comparative Examples 1 to 3, and the skin patch test was carried out for each. The inside of the forearm of the subject was first wiped with 70% ethanol, and after drying completely, 7 kinds of cosmetics were hermetically sealed with 30 μg each. After 24 hours, the specimens were removed and the test site was observed with a magnifying glass after about 30 minutes to confirm the erythema and edema. The evaluation was carried out on the basis of the following Table 4, and the average skin reactivity was calculated according to the following equation (3). The results are shown in Table 5 below.

score Evaluation standard 0 No reaction 0.5 Faint or mild erythema One There is a clear borderline but weak erythema 2 Pronounced erythema 3 Severe erythema and vesicles

Test substance score Reactivity 0 0.5 One 2 3 Example 1 - 4 people - - - 5.1 Example 2 - 4 people 1 person - - 7.7 Example 3 - 3 people 1 person - - 6.4 Example 4 - 3 people 2 people - - 7.7 Comparative Example 1 - 8 people 2 people - - 15.4 Comparative Example 2 - 9 people 1 person - - 14.1 Comparative Example 3 - 8 people 1 person - - 12.8 Control sample - 10 people 1 person - - 15.4

In the results shown in Table 5, Examples 1 to 4 using two or more intercellular-space-blocking substances showed superior skin-soothing effects on irritation induced by stimulating substances as compared with the control samples, It was confirmed that Comparative Examples 1 to 3 were not significantly different from the control samples. In addition, comparing Example 1 with Examples 2 to 4, it was found that the use of all three types of intercellular space-blocking substances increased the effect.

Claims (4)

1. A cosmetic composition for skin soothing and skin irritation which contains, as an active ingredient, a mixture of oleic acid, glycitric acid and oleamide,
Wherein the mixing ratio of oleic acid, glycyrrhetinic acid and oleamide in the mixture is 1: 1: 1 by weight. The method according to claim 1,
Wherein the oleic acid, glycyrrhetinic acid and oleamide are contained in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition. 3. The method according to claim 1 or 2,
The cosmetic composition of the present invention can be used in cosmetics such as softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, powder, makeup base, foundation, Wherein the cosmetic composition is formulated into a body cream, a body oil, a body essence, a body cleanser, a serum, a soap, an emulsion, a lotion, an ointment, a gel, a cream, a patch or a spray.
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