KR20190142655A - Cosmetic composition containg nanoemulsion encapsulated with 7-dehydrocholesterol, cholesterol and stearic acid in inner phase of hyaluronate-ceramide NP complex - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid are encapsulated in an internal phase of a hyaluronic acid-ceramide NP complex. The nanoemulsion having 7-dehydrocholesterol, cholesterol, and stearic acid encapsulated in the internal phase of the hyaluronic acid-ceramide NP complex can increase the stability of the 7-dehydrocholesterol, and through efficient skin absorption, can supply vitamin D without side effects. Furthermore, the nanoemulsion can be beneficially used in a cosmetic composition for a specific purpose of improving a skin condition by using skin lipid components of ceramide NP, cholesterol, and stearic acid.

Description

Cosmetic composition containg nanoemulsion encapsulated with 7-dehydrocholesterol, cholesterol and stearic acid in inner phase of hyaluronate-ceramide, 7-dehydrocholesterol, cholesterol and stearic acid, contain a nanoemulsion encapsulated in the inner phase of the hyaluronic acid-ceramide enpi complex. NP complex}

The present invention relates to a cosmetic composition containing a nanoemulsion in which 7-dehydrocholesterol, cholesterol and stearic acid are enclosed in the inner phase of the hyaluronic acid-ceramide N-P complex, more specifically, 7-dehydrocholesterol, ceramide N-P, cholesterol, 7-dehydrocholesterol, cholesterol, and stearic acid are hyaluronic acid-ceramides that can be stabilized with nanoemulsion that can be absorbed into the skin at once by mixing stearic acid and hyaluronic acid in a certain ratio to improve skin condition by absorbing it into the skin without side effects. The present invention relates to a cosmetic composition containing a nanoemulsion enclosed in an inner phase of an enpy complex.

The question of how to exist younger and beautifully in the history of mankind is a topic of common interest regardless of gender or age. Therefore, various theories about this aging have been proposed, but the most fundamental cause is ultraviolet.

Ultraviolet rays are divided into A, B, C, etc. according to the length of the wavelength. Ultraviolet rays A (UVA) and ultraviolet rays B (UVB) mainly affect the human body. For example, in older women working in agriculture, faces, necks, etc., which are directly exposed to sunlight, become darker, dry and less elastic, and have fine wrinkles, while the body covered by clothes is white and wrinkled. This is because the ultraviolet rays A and B induce skin aging.

After prolonged exposure to UV rays A and B, the skin epidermis becomes totally dry, dead skin cells thicken, develop fine wrinkles, penetrate the dermis layer below the epidermis and damage the collagen bonds responsible for skin elasticity, thereby reducing skin elasticity. Produces deep wrinkles.

In addition, due to environmental pollution, the harmfulness of ultraviolet rays has been emphasized more and more, the use of sunscreen has become an essential element for modern people to live. The use of these sunscreens caused other problems, which ultimately resulted in a deficiency of vitamin D in the body.

Vitamin D is a very important hormone in the homeostasis of calcium and phosphorus and the musculoskeletal system. Insufficient vitamin D is reported to increase rickets, osteomalacia, osteoporosis, as well as malignant tumors such as breast cancer, colon cancer and prostate cancer, cardiovascular diseases including hypertension, diabetes mellitus, multiple sclerosis, psoriasis, rheumatoid arthritis and tuberculosis.

In addition, vitamin D has recently been known to express not only vitamin D receptor (VDR) but also 1 alpha-hydroxylase (1-hydroxylase) required to convert vitamin D into active form in various tissues and cells in the human body. There is a new light on vitamin D action. These findings suggest that vitamin D may be involved in other tissues or organs other than bone, kidney, or small intestine, or involved in biological processes that are not related to bone or mineral homeostasis, and are supported by laboratory and epidemiological studies.

However, according to the National Health and Nutrition Examination Survey, the average 25 (OH) D concentration in US adults in 2001-2004 was 24ng / mL, down 6ng / mL, compared to 1988-94, with only 23% being 30ng / mL. Maintaining more than mL, various previous cohort studies have shown that between 52 and 77% of subjects are vitamin D deficiency or deficiency.

In addition, researchers at the University of California, Toro University, say that daily use of sunscreens with SPF 15 or higher can reduce vitamin D3 production in the body, leading to vitamin D deficiency.

In addition, in Korea, 92% was less than 30ng / mL and 57% was less than 12ng / mL, according to the survey of menopausal women with osteoporosis. Has become a serious health problem.

Accordingly, a method of pharmacologically administering vitamin D has been used to treat vitamin D deficiency. However, pharmacological vitamin D administration has serious side effects. Because vitamin D is fat-soluble and stored in the body fat, symptoms of toxicity caused by vitamin D may occur for several weeks after treatment is stopped. The most important side effects of pharmacological vitamin D administration are increased calcium and phosphorus absorption in the intestine, Hypercalcemia, hyperphosphatemia and hypercalciuria due to increased bone resorption of the skeleton. In addition, side effects such as gastrointestinal symptoms, neuromuscular symptoms, and thirst may occur with nausea. For example, the use of 1 μg of calcitriol per day for more than one year increases the frequency of hypercalcemia, but because of this, treatment is often not interrupted. If nephrolithiasis or hypercalciuria continues for a long time, nephrolithiasis Renal calcification can also occur, which can lead to poor kidney function. Since pharmacological vitamin D administration has serious side effects, it is safest to consume it as a food or supplement, but until now, researches related to vitamin D mainly deal with metabolism, physiological effects, and analysis methods.

In general, the skin is composed of three parts, stratum corneum, epidermal layer and dermis layer. Skin is the body's primary protective layer that protects organs in the body from changes in temperature and humidity, and stimuli from the external environment such as ultraviolet rays and pollutants. It depends on physical and chemical properties. The stratum corneum consists of keratinocytes mainly composed of proteins called keratin and lipid layers filling the cells. Absorption through keratinocytes is very difficult for the skin to absorb the substance, and the pathway through the lipid layer between keratinocytes is common.

On the other hand, the emulsion (emulsion) refers to a liquid-liquid dispersion system in which at least one liquid phase is dispersed in one liquid phase, in particular, the nanoemulsion is an underwater type emulsion, the particle size of the emulsion droplets is usually 10 ~ 100nm. Nanoemulsions with a particle size of 100 ~ 200nm are less affected by gravity than macroemulsions of 1㎛ or more. As a result, the movement between the emulsified particles follows the Brownian motion mainly in the diffusion motion, so that the creaming or precipitation phenomenon which affects the stability of the general macroemulsion is not shown, but the coalescence due to the interaction between the particles Or destabilization is caused by Ostwald ripening resulting from solubility differences due to varying particle sizes.

Korean Unexamined Patent Publication No. 10-2001-0002281 discloses a cosmetic composition containing 7-dehydrocholesterol stabilized using a porous lipophilic polymer resin, and the Republic of Korea Patent Publication No. 10-2010-0039937 A cosmetic composition containing a solid wax microcapsule containing a functional active ingredient such as 7-dehydrocholesterol is disclosed.

But. Without a separate emulsifier, the water-soluble substance hyaluronic acid is not yet developed a technology for capsule emulsification through ion bonding with a poorly soluble ceramide n-py.

Therefore, the present inventors can stabilize the nanoemulsion that can be absorbed into the skin at once by mixing 7-dehydrocholesterol, ceramide N-P, cholesterol, stearic acid and hyaluronic acid to improve the skin condition by absorbing it into the skin without side effects. The present invention was completed by developing a method for preparing a nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid, which have very good efficacy, are encapsulated in the inner phase of a hyaluronic acid-ceramide enpi complex.

Republic of Korea Patent Publication No. 10-2001-0002281 (2001.01.15) Republic of Korea Patent Publication No. 10-2010-0039937 (2010.04.19)

The present invention improves the stability of 7-dehydrocholesterol to effectively supplement vitamin D through side effects through effective absorption and at the same time using the skin lipid components of ceramide n-pi, cholesterol and stearic acid, 7-dehydrocholesterol, It is an object to provide a cosmetic composition containing a nanoemulsion in which cholesterol and stearic acid are enclosed in the inner phase of the hyaluronic acid-ceramide enpi complex.

In order to achieve the above object, the present invention is stabilized by a nano-emulsion that can be absorbed into the skin at once by mixing 7-dehydrocholesterol, ceramide N-P, cholesterol, stearic acid and hyaluronic acid to the skin without side effects It is characterized by providing a cosmetic composition containing a nanoemulsion in which the 7-dehydrocholesterol, cholesterol and stearic acid, which can be absorbed to improve the skin condition, are enclosed in the inner phase of the hyaluronic acid-ceramide NEP complex.

Hereinafter, the present invention will be described in detail.

The combination of Ceramide NEP, Cholesterol and Stearic Acid is an intercellular lipid component of the skin that prevents skin moisture loss and protects foreign substances from the surface of the skin.It is well crystallized in the cosmetic formulation, so it is high in the cosmetic formulation. In the case of containing, layer separation or precipitation phenomenon occurs, or the stability is low, and gelation occurs when left at room temperature for a long time. Thus, cathelicidine promoters that solve the problem of ceramide endothelial, cholesterol and stearic acid combinations, enhance the physical function of skin barriers, induce gene transcription of skin loricrin, involucrin and filaggrin, and inhibit skin microbial propagation. The 7-dehydrocholesterol, which assists in the activity of the promoter, was intended to help improve the skin barrier as well as the physical barrier. However, since 7-dehydrocholesterol is also a poorly soluble substance and low in stability, it is a raw material that is frequently precipitated even when only a small amount is used. In order to solve the problem, the present invention provides a fixed ratio of 7-dehydrocholesterol, ceramide NEP, cholesterol, stearic acid and hyaluronic acid. It was developed a method of preparing a nanoemulsion that can be absorbed into the skin at once by mixing.

First, a method of preparing a nanoemulsion in which 7-dehydrocholesterol (provitamin D3), cholesterol and stearic acid are encapsulated in the inner phase of a hyaluronic acid-ceramide en complex is described.

Dissolving hyaluronate in purified water and adjusting pH with sodium hydroxide (NaOH)

The aqueous hyaluronic acid (hyaluronate) is dissolved in purified water with stirring at 70 ° C. to make the acid portion of the hyaluronic acid anionic, and the pH is adjusted to 8-8.5 by adding sodium hydroxide (NaOH).

Ethanol dissolution of ceramide NP

The poorly soluble ceramide NP is transparently dissolved in ethanol to render the amide bond present in the ceramide end head weakly cationic.

Preparation of hyaluronate-ceramide NP complex

The aqueous solution of the anionic hyaluronic acid and the ethanol in which the weak cationic crystalline ceramide NEP was dissolved were stirred at 70 ° C. for 2 hours to allow the hyaluronic acid and the ceramide NEP to ionize to form a natural micelle. After evaporation of ethanol to prepare a hyaluronic acid-ceramide NP complex in a transparent liquid form.

Encapsulating 7-dehydrocholesterol, cholesterol and stearic acid in the inner phase of the hyaluronate-ceramide NP complex

7-dehydrocholesterol (7-dehydrocholesterol): cholesterol (cholesterol): stearic acid (stearic acid) is dissolved in 20ml of ethanol in a weight ratio of 0.3: 1: 1, and then in the liquid form hyaluronic acid-ceramide n-P complex Slowly infusion causes the fat-soluble substances 7-dehydrocholesterol, cholesterol and stearic acid to gather in the same fat-soluble substance ceramide n-py and penetrate naturally into the hyaluronic acid-ceramide n-pi complex.

In this state, the mixture was stirred at 70 ° C. for 2 hours to evaporate ethanol to finally prepare a nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid were encapsulated in the inner phase of the hyaluronic acid-ceramide enphi complex.

In this case, for the formulation stability of the cosmetic composition 7-dehydrocholesterol (7-dehydrocholesterol): cholesterol (cholesterol): stearic acid (stearic acid): ceramide NP (ceramide NP) in a weight ratio of 0.3: 1: 1: 3 desirable.

Cosmetic compositions containing a nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid are encapsulated in the hyaluronate-ceramide NP complex are commonly used. It depends on the manufacturing method of phosphorus cosmetic composition.

That is, purified water, polyol, thickener and the like are mixed and then heated to 75-80 ° C. to dissolve and homogenized to prepare an aqueous phase. The mixture is mixed with a surfactant, etc., and then heated to 75-85 ° C. to dissolve. Homogenization produces an oil-phase. After adding the oil phase to the water phase and homogenizing by stirring at a rotational speed of 3,000 to 4,000 rpm with HOMO, the neutralizing agent and the preservative were mixed and cooled to 30 to 40 ° C. or less, and then the hyaluronic acid-ceramide NP complex (hyaluronate-ceramide NP complex). 7-dehydrocholesterol (7-dehydrocholesterol), cholesterol (cholesterol) and stearic acid (natea emulsion) encapsulated in the interior of the cosmetic composition is prepared.

At this time, the hyaluronic acid-ceramide NP complex of the present invention (7-dehydrocholesterol), cholesterol (cholesterol) and stearic acid (stearic acid) encapsulated in the inner phase of the hyaluronate-ceramide NP complex The cosmetic composition may further include any one or more of plant-derived extracts, plant-derived oils, and sunscreen ingredients.

The plant-derived extracts include French coastal pine bark extract, balloon vine flower, leaf and vine extract, turmeric root extract, purslane extract, buckwheat extract, Mexican chia seed extract, chamomile flower extract, persimmon leaf extract, water lily flower extract, calendula flower extract It is preferable that at least one selected from among the extracts of the plant extract, shea butter seed cake extract, green tea extract, Western pine leaf leaves and stem extracts, sunflower seed extract, portulaca extract, Termus thermophilus fermentation, and aloe vera leaf extract.

The plant-derived oil is preferably any one or more selected from broad fragrance oil, bergamot oil, sentinium geranium flower oil, echium plantarginium seed oil and salvia oil.

The sunscreen component is Ethylhexyl Methoxycinnamate, Ethylhexyl Salicylate, Diethylamino Hydroxybenzoyl Hexyl Benzoate, Bis-Ethylhexyloxyphenol Methoxy Phenyl triazine (Bis-ethylhexyloxyphenolmethoxyphenyltriazine), zinc oxide (Zinc Oxide), titanium dioxide (titanium dioxide), octocrylene (Octocrylene), homosalate (Homosalate) is preferably any one or more.

Meanwhile, a nanoemulsion containing 7-dehydrocholesterol, cholesterol, and stearic acid is encapsulated in the hyaluronate-ceramide NP complex of the present invention. The formulation of the cosmetic composition may be any one of an emulsifier type selected from essences, lotions, creams and packs.

The 7-dehydrocholesterol, cholesterol and stearic acid of the present invention improves the stability of the 7-dehydrocholesterol through the nanoemulsion encapsulated in the hyaluronic acid-ceramide NEP complex to supplement vitamin D without side effects through effective absorption to the skin At the same time it can be usefully used in cosmetic compositions for specific purposes that can improve the skin condition by using the skin lipid components of ceramide N-P, cholesterol and stearic acid.

1 is a graph showing the skin moisture content test results of a cosmetic composition containing a nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid of the present invention are encapsulated in a hyaluronic acid-ceramide NEP complex.
Figure 2 is a graph showing the pigmentation test results of the skin epidermis of the cosmetic composition containing a nano-emulsion in which 7-dehydrocholesterol, cholesterol, stearic acid of the present invention is encapsulated in the hyaluronic acid-ceramide NEP complex.
Figure 3 is a graph showing the pigmentation test results of the skin dermis of the cosmetic composition containing the nano-emulsion of 7-dehydrocholesterol, cholesterol, stearic acid encapsulated in the hyaluronic acid-ceramide NEP complex of the present invention.
Figure 4 is a graph showing the skin tone brightness test results of the cosmetic composition containing a nano-emulsion of 7-dehydrocholesterol, cholesterol, stearic acid encapsulated in the hyaluronic acid-ceramide NEP complex of the present invention.
5 is a graph showing a test result of the improvement of the skin wrinkles of the cosmetic composition containing a nano-emulsion of 7-dehydrocholesterol, cholesterol, stearic acid encapsulated in the hyaluronic acid-ceramide NEP complex of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples do not limit the scope of the present invention, and ordinary changes by those skilled in the art are possible within the scope of the technical idea of the present invention.

<Example 1>

Preparation of Nanoemulsion with 7-dehydrocholesterol, Cholesterol and Stearic Acid Encapsulated in the Inner Phase of Hyaluronic Acid-Ceramide Enpi Complex

1-1. Nanoemulsion 1

The nanoemulsion was prepared such that the weight ratio of sodium hyaluronate: ceramide npy: cholesterol: stearic acid: 7-dehydrocholesterol was 10: 3: 1: 1: 0.3.

That is, 1 g of sodium hyaluronate is dissolved in 90 g of purified water with stirring at 70 ° C. to make the acid portion of hyaluronic acid anionic, and then sodium hydroxide (NaOH) is added to pH 8. Titrated to ˜8.5.

Meanwhile, 300 mg of ceramide NP was transparently dissolved in 20 ml of ethanol to make the amide bond present in the ceramide nipple head weakly cationic.

Then, the anionic, hyaluronic acid aqueous solution and the ethanol in which the weakly cationic ceramide N-P was dissolved were stirred at 70 ° C. for 2 hours to allow the hyaluronic acid and ceramide N-I to be ion-bonded to form a natural micelle (micelle). ) Was formed.

Then, ethanol was evaporated to prepare a hyaluronic acid-ceramide NP complex in a transparent liquid form.

Then, 30 mg of 7-dehydrocholesterol, 100 mg of cholesterol and 100 mg of stearic acid were dissolved in 20 ml of ethanol in proportion, and then slowly added to the liquid hyaluronic acid-ceramide n-P complex. The fat-soluble substance 7-dehydrocholesterol, cholesterol and stearic acid were gathered in the same fat-soluble substance ceramide n-py to penetrate into the hyaluronic acid-ceramide n-pi complex. In this state, the mixture was stirred at 70 ° C. for 2 hours to evaporate ethanol to prepare a nanoemulsion in which 7-dehydrocholesterol, cholesterol, and stearic acid were finally contained in the hyaluronic acid-ceramide nP complex.

1-2. Nanoemulsion 2

A nanoemulsion was prepared in the same manner as in Example 1-1, except that the weight ratio of sodium hyaluronate: ceramide npy: cholesterol: stearic acid: 7-dehydrocholesterol was 13: 3: 1: 1: 0.03. .

<Examples 2 to 4>

Preparation of Cosmetic Composition Containing Nanoemulsion with 7-dehydrocholesterol, Cholesterol and Stearic Acid Encapsulated in the Inner Body of Hyaluronic Acid-Ceramide Enpi Complex

The cosmetic composition containing nanoemulsion 1 in which the 7-dehydrocholesterol, cholesterol, and stearic acid of Example 1-1 was encapsulated in the inner layer of the hyaluronic acid-ceramide N-P complex was prepared using the composition shown in Table 1 below. It was prepared by the method.

Comparative Example 1

Preparation of Cosmetic Composition Containing Nanoemulsion with 7-dehydrocholesterol, Cholesterol and Stearic Acid Encapsulated in the Inner Body of Hyaluronic Acid-Ceramide Enpi Complex

The cosmetic composition containing nanoemulsion 2 in which the 7-dehydrocholesterol, cholesterol and stearic acid of Example 1-2 were encapsulated in the inner layer of the hyaluronic acid-ceramide n-py complex of Example 1-2 was prepared in the conventional composition of the cosmetic composition. It was prepared by the method.

Composition ratio of cosmetic composition including nanoemulsion (unit: weight%) Example 2 Example 3 Example 4 Comparative Example 1 Award Water 43.79 45.79 40.79 40.79 Butylene glycol 4 Disodium EDTA 0.05 The upper part Polyglyceryl 3 Stearate 0.4 Cetearyl ollivate 0.3 SorbitanStearate One Dimethicone 6 Cetearyl alcohol 1.2 Dimethicone /
Vinyl dimethicone crosspolymer One Phospholipids 0.6 C12-15 Alkyl Benzoate 3.12 Thickener Carbomer 0.4 Sodium Acrylate /
Sodium Acryloydimethyl taurate Copolymer 0.3 antiseptic 1,2Hexandiol 1.5 Efficacy
(Awards) Centella Asiatica Extract 0 3 Efficacy
(Charged) Pollen extract 0.2 0 0.2 Glycine Soja (Soybean) Oil Unsaponifiables 1.2 0 1.2 Olea Europaea (Olive) Oil Unsaponifiables 2.4 0 2.4 Triticum Vulgare (Wheat) Germ Oil Unsaponifiables 1.2 0 1.2 Functional Notice
Raw material Adenosine 0.04 Niacinamide 2 Efficacy
(Ultraviolet ray blocker) Ethylhexyl Methoxycinnamate 6.8 Octocrylene 3 Homosalate 2.5 Diethylamino Hydroxybenzoyl Hexyl Benzoate 3 Titanium Dioxide 13 Nanoemulsion 1 of Example 1-1 One - - - - One - - - - One - Nanoemulsion 2 of Example 1-2 - - - One

<Test Example 1>

Stability Test of Nanoemulsion with 7-dehydrocholesterol, Cholesterol and Stearic Acid Encapsulated in the Inner Phase of Hyaluronic Acid-Ceramide Enpi Complex

Anionic and hyaluronic acid aqueous solution and weakly cationic ceramide N-P are dissolved in ethanol, and hyaluronic acid and ceramide N-P are formed through micelles to form micelles. Ionic bonds may be broken during the preparation of the hyaluronic acid-ceramide nP complex. Therefore, in order to confirm the stability of the nanoemulsion of Example 1 according to the composition ratio of sodium hyaluronate: ceramide nP: cholesterol: stearic acid: 7-dehydrocholesterol and stored for 4 weeks at room temperature 25 ℃ and 45 ℃ Formulation stability over time was measured according to visual stability.

The results are shown in Table 2 below.

3: maintenance of emulsion formulation, 2: discoloration or odor occurrence, 1: precipitation or delamination

As can be seen in Table 2 below, regardless of the composition ratio of sodium hyaluronate: ceramide N: Cholesterol: stearic acid: 7-dehydrocholesterol, the nanoemulsion of Example 1 without precipitation at room temperature 25 ℃ and 45 ℃, respectively It was stable.

Nano Emulsion Nanoemulsion 1 of Example 1-1 Nanoemulsion 2 of Example 1-2 25 ℃ 1 day 3 3 3 days 3 3 5 days 3 3 7 days 3 3 2 weeks 3 3 3 weeks 3 3 4 Weeks 3 3 45 ℃ 1 day 3 3 3 days 3 3 5 days 3 3 7 days 3 3 2 weeks 3 3 3 weeks 3 3 4 Weeks 3 3

<Test Example 2>

Formulation Stability Test

Ceramide N-P, cholesterol, stearic acid and 7-dehydrocholesterol are poorly soluble materials, so low precipitation is a raw material frequently precipitated using only a small amount of hyaluronic acid-ceramide enpi complex of Example 1 In order to confirm the stability at high temperatures of the cosmetic composition containing the nanoemulsion encapsulated in the interior of each of the cosmetic compositions of Examples 2 to 4 and Comparative Example 1 was stored for 4 weeks at 45 ℃ to the visual stability of the following criteria According to the formulation stability was measured over time.

The results are shown in Table 3 below.

5: cream formulation; 4: no delamination but change in appearance; 3: discoloration or deodorization; 2: precipitation; 1: layer separation

As can be seen in Table 3, the cosmetic composition after 4 weeks showed changes and discoloration of the properties of the nanoemulsion of Example 1 according to the composition ratio of ceramide n-pi, cholesterol, stearic acid and 7-dehydrocholesterol, The layer separation phenomenon was also observed.

In particular, it can be seen that Example 2, Example 3 and Example 4 containing the nanoemulsion 1 of Example 1-1 maintained the stable formulation of the creamy phase for 4 weeks.

Formulation Stability at High Temperature Comparative Example 1 Example 2 Example 3 Example 4 1 day 5 5 5 5 3 days 5 5 5 5 5 days 5 5 5 5 7 days 5 5 5 5 2 weeks 4 5 5 5 3 weeks 4 5 5 5 4 Weeks 3 5 5 5

<Test Example 3>

Skin moisture test

Twenty four panelists consisting of four 20's, 22's 30's, 2's 40's, and 2 males and 26 females by gender, twice a day, face and body skin for 4 weeks, Comparative Example 1 And the cosmetic composition of Examples 2 to 4 was applied to soak well.

In order to measure the exact condition of the skin condition, the panel is stabilized for 30 minutes in the skin diagnosis room equipped with a constant temperature and humidity system with a temperature of 25 ± 2 ° C and a humidity of 40-60%. The water intake was then restricted for accurate evaluation.

Transdermal moisture loss (TEWL) was measured once every 5 times in week 0, week 1, week 2, week 3, and week 4 using the Vapometer (Delfin) for each part of the forehead, cheeks, nose and chin of the panel. The measurement was carried out for 10 seconds, and the measurement site was photographed in order to obtain an objective result, and the location was marked so that the same site could be measured in the next measurement.

The results are shown in Figure 1 and Table 4.

As can be seen in Figure 1 and Table 4, Comparative Example 1 has almost no change in the moisture content, while Example 2, Example 3 and Example 4 was found to increase the moisture content. This is because 7-dehydrocholesterol, cholesterol and stearic acid absorbed through the cosmetic composition containing nanoemulsion 1, which maintained the stable formulation of the creamy phase of Example 1-1, reduced skin moisture loss while improving skin barrier function. Because.

On the other hand, it was found that the highest moisturizing effect in the high skin moisture content in Example 4. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

Skin moisture content Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 52.85 53.49 50.86 51.79 54.12 Example 2 43.76 46.91 50.71 57.74 55.53 Example 3 48.59 51.95 55.34 59.39 60.84 Example 4 36.59 43.31 44.03 48.77 53.80

<Test Example 4>

Pigmentation test of skin epidermis

Pigmentation measurement of the skin epidermis was tested in the same manner as in Test Example 3 except that JANUS-II was used.

The results are shown in FIG. 2 and Table 5.

As can be seen in Figure 2 and Table 5, Comparative Example 1, Example 2, Example 3 and Example 4, since the sunscreen in common, the pigmentation was improved when used continuously for 4 weeks.

However, Comparative Example 1 contains a sunscreen agent, but the ultraviolet rays are blocked, but the cream properties of the above Example 1-1 to prevent skin damage due to ultraviolet rays and thermal energy generated during the UV blocking process While the pigmentation improvement effect was not large because the nanoemulsion 1 containing the stable formulation of the present invention was not contained, Examples 2, 3 and 4 maintained the stable formulation of the creamy form of Example 1-1. Nanoemulsion 1 is contained, thereby increasing the pigmentation improvement width.

On the other hand, in Example 4 it was found that the pigmentation of the epidermis was improved more quickly and effectively. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

Pigmentation (PL) Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 0.91 0.88 0.90 0.85 0.86 Example 2 1.09 1.06 1.06 1.04 0.99 Example 3 1.12 1.06 1.01 1.01 0.95 Example 4 1.31 1.19 1.13 1.11 1.09

<Test Example 5>

Pigmentation test of skin dermis

Pigmentation measurement of the dermis of the skin was tested in the same manner as in Test Example 3 except that JANUS-II was used.

The results are shown in FIG. 3 and Table 6.

As can be seen in Figure 3 and Table 6, Comparative Example 1 contains a sunscreen, but the ultraviolet light is blocked, but the function to prevent skin damage due to ultraviolet rays and heat energy generated during the UV blocking process While there is no pigmentation of dermal dermis since the nanoemulsion 1, which maintains the stable formulation of the creamy form of Example 1-1, does not contain pigments, Example 2-1, Example 3 and Example 4, Nanoemulsion 1, which maintained a stable formulation of creamy phase, was contained, so that slight changes in the pigmentation of the dermis could be confirmed.

In particular, compared to Examples 2 and 3, Example 4 showed a continuous decrease in pigmentation of skin dermis in a weekly measurement result, and after 4 weeks, the pigmentation reduction rate was increased by 11% compared to Week 0. there was. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

Pigmentation (UV) Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 0.77 0.74 0.78 0.72 0.77 Example 2 1.75 1.67 1.65 1.60 1.60 Example 3 1.12 1.10 1.10 1.06 1.06 Example 4 1.37 1.30 1.28 1.26 1.21

<Test Example 6>

Skin tone brightness test

The brightness measurement of the skin tone was tested in the same manner as in Test Example 3, except that JANUS-II was used.

The results are shown in FIG. 4 and Table 7.

As can be seen in Figure 4 and Table 7, Comparative Example 1 does not contain nanoemulsion 1, which maintains the stable formulation of the creamy form of Example 1-1, niacinamide (niacinamide) as a functional whitening notice component While the skin tone of the early skin was darkened due to no synergistic effect, Examples 2, 3, and 4 contained Nanoemulsion 1, which maintained a stable formulation of the creamy form of Example 1-1. Synergistic effect with niacinamide, a noticeable ingredient, brightens skin tone.

Particularly, in Example 2 and Example 3, the skin tone became brighter after 4 weeks, but after a certain time, the skin tone was no longer brightened, whereas Example 4 maintained the state in which the skin tone became brighter. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

Skin tone Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 1.09 1.08 1.10 1.10 1.10 Example 2 1.04 1.04 1.03 1.02 1.03 Example 3 0.97 0.96 0.95 0.95 0.94 Example 4 1.06 1.06 1.04 1.02 1.01

<Test Example 7>

Skin wrinkle improvement test

Measurement of the improvement of the skin wrinkles was tested in the same manner as in Test Example 3, except that JANUS-II was used.

The results are shown in FIG. 5 and Table 8.

As can be seen in Figure 5 and Table 8, Comparative Example 1 does not contain Nanoemulsion 1, which maintains the stable formulation of the creamy form of Example 1-1, synergistic with adenosine (adenosine), a functional whitening notice component Wrinkle improvement effect was not maintained after 2 weeks, so the wrinkles increased rather than week 0, whereas in Example 2, Example 3 and Example 4, the creamy formulation of Example 1-1 maintained a stable formulation. One nanoemulsion 1 contains synergistic effect with adenosine, which is a functional whitening notice component, and the wrinkle improvement effect was increased the most at 3 weeks, and the wrinkle improvement effect was continued until 4 weeks.

In particular, the wrinkle improvement effect was larger in Example 4 than Example 2 and Example 3. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

wrinkle Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 0.58 0.52 0.49 0.58 0.65 Example 2 0.59 0.49 0.44 0.35 0.41 Example 3 1.22 1.18 1.16 1.12 1.13 Example 4 1.70 1.62 1.55 1.41 1.40

<Test Example 8>

Skin elasticity improvement test

Skin elasticity improvement was measured using the Ballistometer (Ballistometer BLS780, Dia-Stron Ltd., UK), and was tested in the same manner as in Test Example 3 except that the right ball of the panel was measured.

The results are shown in Table 9.

As can be seen in Table 9 below, Comparative Example 1 does not contain Nanoemulsion 1, which retained the stable formulation of the creamy form of Example 1-1, and thus, together with a sunscreen, harmful substances such as ultraviolet rays (ultraviolet rays, etc.). Skin elasticity is increased up to 2 weeks, while skin elasticity is decreased up to 4 weeks, whereas Examples 2, 3 and 4 are nanoemulsions that maintain the stable formulation of the creamy form of Example 1-1. 1 contained synergistic effect with sunscreen, skin elasticity increased up to 4 weeks.

On the other hand, Example 4 it was found that the sustained improvement of skin elasticity until the fourth week. It does not contain the water-soluble Centella Asiatica Extract in Example 2, and Example 3 does not contain the oil-soluble Pollen Extract, Soybean oil unsaponifiables, Olive Oil unsaponifiables, wheat germ oil unsaponifiables, while Example 4 contains both the water-soluble and fat-soluble extracts to maintain the creamy stable formulation of Example 1-1 This is because it showed synergy with emulsion 1.

Skin elasticity Week 0 1 week 2 weeks 3 weeks 4 Weeks Comparative Example 1 0.82 0.90 0.96 0.88 0.86 Example 2 0.35 0.41 0.44 0.49 0.59 Example 3 0.49 0.52 0.58 0.58 0.65 Example 4 0.55 0.62 0.70 0.80 0.96

Claims (8)

A cosmetic composition comprising a nanoemulsion consisting of 7-dehydrocholesterol, ceramide NP, cholesterol, stearic acid and hyaluronate.
The method of claim 1,
The nanoemulsion is characterized in that 7-dehydrocholesterol, cholesterol, and stearic acid are encapsulated in the inner phase of the hyaluronate-ceramide NP complex. Cosmetic composition to be.
The method of claim 1,
The nano-emulsion 7-dehydrocholesterol (7-dehydrocholesterol): cholesterol (cholesterol): stearic acid (stearic acid): ceramide NP (ceramide NP) is a cosmetic composition, characterized in that consisting of a weight ratio of 0.3: 1: 1: 3 .
The method of claim 1,
The cosmetic composition is a cosmetic composition, characterized in that it has any one emulsifier type selected from essence (essence), lotion (lotion), cream (cream) and pack (pack).
The method according to any one of claims 1 to 3,
The cosmetic composition is a cosmetic composition, characterized in that it further comprises any one or more of plant-derived extracts, plant-derived oils and sunscreen ingredients.
The method of claim 5,
The plant-derived extracts include French coastal pine bark extract, balloon vine flower, leaf and vine extract, turmeric root extract, purslane extract, buckwheat extract, Mexican chia seed extract, chamomile flower extract, persimmon leaf extract, water lily flower extract, calendula flower extract Cosmetic composition, characterized in that at least one selected from, Centella asiatica extract, Shea butter seed cake extract, Green tea extract, Western pine tree leaves / stem extract, Sunflower seed extract, Portulaca extract, Termus thermophilus fermentation, Aloe vera leaf extract .
The method of claim 5,
The plant-derived oil is a cosmetic composition, characterized in that any one or more selected from broad fragrance oil, bergamot oil, sentiranium flower oil, Echium plantation genium seed oil, salvia oil.
The method of claim 5,
The sunscreen component is Ethylhexyl Methoxycinnamate, Ethylhexyl Salicylate, Diethylamino Hydroxybenzoyl Hexyl Benzoate, Bis-Ethylhexyloxyphenol Methoxy Cosmetic composition, characterized in that any one or more selected from phenyltriazine (Bis-ethylhexyloxyphenolmethoxyphenyltriazine), zinc oxide (Zinc Oxide), titanium dioxide (titanium dioxide), octocrylene, homosalate.

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