KR101740502B1 - Pharmaceutical composition for relieving hangover which comprises extract of Cedrela sinensis A. Juss. as an active component - Google Patents
Pharmaceutical composition for relieving hangover which comprises extract of Cedrela sinensis A. Juss. as an active component Download PDFInfo
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- KR101740502B1 KR101740502B1 KR1020150157776A KR20150157776A KR101740502B1 KR 101740502 B1 KR101740502 B1 KR 101740502B1 KR 1020150157776 A KR1020150157776 A KR 1020150157776A KR 20150157776 A KR20150157776 A KR 20150157776A KR 101740502 B1 KR101740502 B1 KR 101740502B1
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- extract
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- alcohol
- juss
- pharmaceutical composition
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/58—Meliaceae (Chinaberry or Mahogany family), e.g. Azadirachta (neem)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/334—Foods, ingredients or supplements having a functional effect on health treating the effects of consuming alcohol, narcotics or other addictive behavior, e.g. treating hangover or reducing blood alcohol levels
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Abstract
The present invention relates to a method for producing sinensis A. Juss.) Extract as an active ingredient. Specifically, a 70% fermented alcoholic extract of Leuconostoc japonica was obtained. The extract of Leucocybe was treated with liver cell line, As a result, it was confirmed that the survival rate of cells was increased, and that the extract of japanese japonica extract increased the activity of alcoholase, so that the japanese japonica extract of the present invention can be effectively used as a composition for preventing hangover.
Description
The present invention relates to a method for producing sinensis A. Juss.) extract as an active ingredient, it is possible to develop related fields such as medicines and foods for improving the inconvenience caused by drinking.
The hanging phenomenon is caused by the toxicity of alcohol (ethyl alcohol) and / or acetaldehyde accumulated in liver cells, and the toxicity of alcohol and / or acetaldehyde is prolonged for a long time to cause a disorder of metabolism and the like, Abdominal bloating, and vomiting.
In the process of alcohol metabolism, the alcohol introduced into the body is absorbed from the stomach or small intestine, is transferred into the blood vessels, and is transferred to the liver. The alcohol is dehydrogenated by alcohol dehydrogenase (ADH) And the acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase (ALDH), an aldehyde degrading enzyme, in hepatocytes, and finally decomposed into carbon dioxide gas and water
On the other hand, type Ⅱ, which initiates oxidation even when acetaldehyde is low in concentration, and type Ⅰ, which does not work if acetaldehyde is not concentrated at a high concentration. However, since Asian people generally lack or lack Ⅱ type aldehyde decomposing enzyme, The oxidation of acetaldehyde and alcohol is slow, and therefore, the toxicity of acetaldehyde and alcohol, which is not oxidized, is interfered with normal metabolism, so that many hangover phenomena occur.
In this regard, much research has been conducted on the effect of reducing the hangover resolution of a composition prepared by using a natural extract alone or in combination. For example, a hangover remedy using green tea (Korean Patent Registration No. 1005604110000), a hangover treatment using a stone tree extract (Korean Patent Registration No. 1012475240000).
Cedrela Sinensis A. Juss.) is a taxonomic species belonging to the fagaceous tree, and is used for food in Korea and China. Due to its unique flavor, it is resistant to pests and diseases, and it contains various nutrients and has recently been studied as a health functional ingredient. In addition, leaves and fruits have been used as herbal medicines because of their excellent pharmacological action. Recently, antioxidant, anti-inflammation, and immunity enhancement activities have been studied. The main researches to date are as follows: Extracts were immunosuppressive, antimicrobial and analgesic activity after extracting or pulverizing Ojacilia leaves and extracting them with hot water, ethanol, and methanol. In addition, a study on the flavonoid contents of the leaves of Ochuyuki in summer and seasonal flavonoid contents such as quercitrin and afzelin have been reported.
[Patent Document 1] Japanese Patent Application Laid-Open No. 10-20060028826 (Publication Date: Apr. 04, 2006, Patent Application No. 10-2004-0077685, filed on September 30, 2004) And a food composition comprising the same and Korean Patent Publication No. 1020080062794 (Publication date: 2008.07.03, patent application No. 10-2006-0138911, filed on December 29, 2006) Food, "and various studies using actual chamomile trees have been conducted. However, the effect of hangover resolution has not been reported so far.
Accordingly, the inventors of the present invention completed the present invention by studying to find a hungable, sparingly soluble composition in a natural substance, confirming that the extract of Chamaecyparis obtusa inhibits cell damage by alcohol treatment and activates an alcohololytic enzyme.
An object of the present invention is chamjuk tree (Cedrela sinensis A. Juss.) Extract as an active ingredient.
In order to achieve the above object, the present invention chamjuk tree (Cedrela sinensis A. Juss.) Extract as an active ingredient.
Further, the present invention provides a health functional food for hangover marine life containing an extract of Aspergillus oryzae as an active ingredient.
In addition, the present invention provides a pharmaceutical composition for protecting an alcoholic liver injury containing an extract of Aspergillus oryzae as an active ingredient.
Further, the present invention provides a health functional food for protecting against alcoholic liver damage containing an extract of Aspergillus oryzae as an active ingredient.
The present invention relates to a method for producing sinensis A. Juss.) Extract as an active ingredient. Specifically, a 70% fermented alcoholic extract of Leuconostoc japonica was obtained. The extract of Leucocybe was treated with liver cell line, As a result, it was confirmed that the survival rate of cells was increased, and that the extract of japanese japonica extract increased the activity of alcoholase, so that the japanese japonica extract of the present invention can be effectively used as a composition for preventing hangover.
FIG. 1 is Toona sinensis (Cedrela sinensis A. Juss et al.).
FIG. 2 is a graph showing the protective effect of alcoholic hepatocyte injury on the extract of Alnus japonica.
FIG. 3 is a view showing an alcohol metabolism process. FIG.
Fig. 4 is a graph showing the effect of alcohololytic enzymes on the extract of Alnus japonica.
Hereinafter, the present invention will be described in detail.
The present invention relates to a method for producing sinensis A. Juss.) Extract as an active ingredient.
The extract may be prepared by a manufacturing method comprising the following steps.
1) Extracting the almond tree with an extraction solvent;
2) filtering the extract of step 1); And
3) Concentrating the filtered extract of step 2) under reduced pressure and drying.
In the above method, the step 1) can be used without limitation such as cultivated or marketed, and it is possible to use both leaves, stems or roots, but according to a preferred embodiment of the present invention, Do.
In the above method, any conventional method known in the art can be used as an extract method for extracting the alfalfa extract, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction.
In the above method, the extract is preferably extracted with water, C 1 to C 2 lower alcohol, alcohol, or a mixed solvent thereof. According to a preferred embodiment of the present invention, the extract is preferably 70% fermented.
In the above method, shaking extraction, Soxhlet extraction, or reflux extraction may be used as the extraction method. The extraction solvent may be extracted by adding 1 to 40 times the amount of dried oak tree, and the extraction temperature may be 20 to 50 ° C And the extraction time may be 10 to 100 hours, and the number of times of extraction may be 1 to 5 times.
In this method, it is possible to use a vacuum decompression concentrator or a vacuum rotary evaporator for the concentration under reduced pressure in step 3). The drying can be carried out under reduced pressure, vacuum drying, boiling, spray drying or freeze drying.
The above extract is characterized by increasing the cell survival rate by alcohol and increasing the activity of alcohololytic enzyme.
In a specific example of the present invention, the present inventors obtained a 70% fermented alcoholic extract of tamarind tree, treated the tamarind extract with 100 μg / ml of hepatocyte HepG2, induced cell damage with ethanol 1M, As a result, it was confirmed that the cell survival rate was increased when pretreated with the extract of Chamaecyparis obtusa (see FIG. 2). As a result, it was confirmed that the activity of the alcohololytic enzyme, ADH, was increased when the extract of Kochujang extract was treated at a concentration of 500 μg / ml (see FIG. 4) The extract can be usefully used as a pharmaceutical composition for hangover.
The composition of the present invention may further comprise a pharmaceutically acceptable carrier. The term " pharmaceutically acceptable "as used herein means that the composition is free of toxicity to cells or humans exposed to the composition. Compositions comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. The carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, But may be at least one selected from the group consisting of polyvinylpyrrolidone, physiological saline, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol and liquid paraffin, But are not limited to, ordinary carriers, excipients or diluents. The components can be added to the fermented rumba extract as an active ingredient independently or in combination.
Solid formulations for oral administration may include tablet pills, powders, granules, capsules and the like, which may contain one or more excipients, such as starch, calcium carbonate, sucrose or lactose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.
The pharmaceutical composition of the present invention may also be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, Or a pharmaceutically acceptable salt thereof.
The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. There is no particular restriction on the dosage, and it may vary depending on the body's absorption, body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, severity of disease and the like. The pharmaceutical composition of the present invention is prepared in consideration of an effective dose range, and the unit dosage formulations thus formulated are classified according to the judgment of the expert who monitors or observes the administration of the drug, if necessary, Or may be administered several times at a predetermined time interval. Preferably, the composition of the present invention may be administered at a dose of 0.5 to 5000 mg / kg, preferably 50 to 500 mg / kg, more preferably 50 mg / kg, per day based on the amount of the extract. , And the administration may be carried out once a day or several times.
Further, the present invention provides a health functional food for hangover marine life containing an extract of Aspergillus oryzae as an active ingredient.
It is preferred that the leaves are extracted with a 70% fermented alcoholic beverage.
In a specific example of the present invention, the inventors of the present invention produced extracts of Chamaecyparis obtusa (Chamaecyparis obtusa) and the extracts of Chamaecyparis obtusa mitigated the decrease of cell viability by ethanol in liver cell lines (see Fig. 2) and increased the activity of alcohololytic enzymes (See FIG. 4), the extract of Chamaeus japonica extract of the present invention can be usefully used as a food composition for hangover and a health functional food.
The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau Martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate may be selected from the range of 0.01 to 0.04 part by weight, specifically about 0.02 to 0.03 part by weight per 100 parts by weight of the health functional food of the present invention.
In addition to the above, the health functional food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, A carbonating agent used in a carbonated beverage, and the like. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health food of the present invention.
In addition, the present invention provides a pharmaceutical composition for protecting an alcoholic liver injury containing an extract of Aspergillus oryzae as an active ingredient.
Further, the present invention provides a health functional food for protecting against alcoholic liver damage containing an extract of Aspergillus oryzae as an active ingredient.
The extract is characterized by increasing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).
The alcoholic liver damage is characterized by acute or chronic alcoholic liver disease (ALD) and alcoholic hepatitis.
In a specific example of the present invention, the inventors of the present invention produced extracts of Chamaecyparis obtusa (Chamaecyparis obtusa) and the extracts of Chamaecyparis obtusa mitigated the decrease of cell viability by ethanol in liver cell lines (see Fig. 2) and increased the activity of alcohololytic enzymes (Refer to FIG. 4), the extract of L. japonica extract of the present invention can be usefully used as a pharmaceutical composition for protecting against alcoholic liver damage and a health functional food.
Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.
However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.
< Example 1> Manufacture of jasmine tree extract
Cedrela sinensis A. Juss.) Extracts were purchased from Chonnam Hainan Agricultural Technology Center.
Specifically, the alien substance of oak tree leaves was removed, and a 70% fermented alcoholic beverage was added to 30 g of oak yam, and the mixture was stirred at room temperature for 16 hours, and filtered to filter paper. To the residue, 70% fermented alcohol was added 10 times and the mixture was stirred at room temperature for 3 hours. The mixture was filtered on filter paper, and then combined with the first extract, concentrated under reduced pressure at 34 ° C, and lyophilized to prepare the final extract.
The extraction yield was 34.2% and the lyophilized weight was calculated as a percentage of the weight of the sample used in the extract preparation.
< Example 2> Cell culture
Hepatocyte HepG2 cells were cultured by the following method in order to confirm the effect of protecting the liver cells from the extract of Chamaecyparis obtusa.
Specifically, the cells were cultured in a 5% CO 2 incubator at 37 ° C. in DMEM supplemented with 10% FBS (fetal bovine serum) and 100 IU / ml penicillin (Dulbecco's modified Eagle's medium, Gibco, USA) They were maintained on a quarterly basis.
< Experimental Example 1> Confirmation of hepatocyte protective effect of extract
The protective effect of hepatocyte on the extract of japanese japonica prepared in Example 1 was confirmed.
Specifically, the hepatocyte HepG2 cultured by the method of Example 2 was cultured on a plate at a concentration of 7 × 10 3 / well for 24 hours, and then treated with a 100 μg / ml concentration of Ochuyuki leaf extract. After 1 hour, the cells were treated with 1 M of ethanol and cultured for 17 hours to induce cell damage. Cell viability was then measured using the MTS assay method. Specifically, each cell plate well was treated with MTS (Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay) reagent and the degree of color development was measured at 485 nm using an Envision microplate reader (Perkin Elmer). Cell viability was calculated based on the 100% survival rate of the untreated control (control).
As a result, as shown in FIG. 2, the cell survival rate was reduced by 57% as compared with the ethanol-treated cell by treatment with ethanol 1M (FIG. 2). On the other hand, when pretreatment of Ochuyuki leaf extract was performed 1 hour before ethanol treatment, the cell survival rate decreased only by about 23% (Fig. 2). It can be seen that the extract of Chachoengesus japonica protects hepatocyte damage caused by ethanol by more than 50%.
< Experimental Example 2> Effect of alcohol extract on the activity
As shown in FIG. 3, alcohol is decomposed into acetaldehyde by alcohol dehydrogenase (ADH), which is finally decomposed into acetate by aldehyde dehydrogenase (ALDH). The alcohololytic enzyme activity of the japanese japonica extract prepared in Example 1 was tested by the following method.
ADH and ALDH activities were measured by Alcohol Dehydrogenase Activity Colorimetric Assay Kit (Biovision, USA) and Aldehyde Dehydrogenase Activity Colorimetric Assay Kit (Biovision, USA). 100 μl of a yeast extract (500 μg / ml) and 100 μl of a reagent (developer, substrate, buffer) were added to a 96-well plate and reacted at 37 ° C for 3 minutes. The initial absorbance (A0) was measured at 450 nm. After incubation at 37 ° C for 30 minutes, absorbance (A1) was measured at 450 nm. The absorbance (A1-A0) before and after the reaction was substituted into the standard curve, and the amount of NADH was measured. The ADH activity was measured according to the following equation shown in the kit and expressed as% of the untreated control (control).
[ Equation 1 ]
As a result, as shown in FIG. 4, when the extract was treated at a concentration of 500 μg / ml, the activity of ADH as an alcoholase was increased by 20% and the activity of ALDH was increased by 12% (FIG. 4).
Claims (6)
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| CN103598364A (en) * | 2013-10-29 | 2014-02-26 | 安徽博格生物科技有限公司 | Tea product capable of dispelling effects of alcohol and protecting liver, and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| YAN, SHENG-LEI et al., ‘Protective effects of maslinic acid against alcohol-induced acute liver injury in mice’, Food and Chemical Toxicology 74 (2014) 149-155* |
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| KR20170055095A (en) | 2017-05-19 |
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