KR101708250B1 - Pharmaceutical composition for relieving hangover and protecting liver which comprises extract of Heracleum moellendorffii HANCE as an active component - Google Patents
Pharmaceutical composition for relieving hangover and protecting liver which comprises extract of Heracleum moellendorffii HANCE as an active component Download PDFInfo
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- KR101708250B1 KR101708250B1 KR1020150157777A KR20150157777A KR101708250B1 KR 101708250 B1 KR101708250 B1 KR 101708250B1 KR 1020150157777 A KR1020150157777 A KR 1020150157777A KR 20150157777 A KR20150157777 A KR 20150157777A KR 101708250 B1 KR101708250 B1 KR 101708250B1
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- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229940032147 starch Drugs 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000002511 suppository base Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/334—Foods, ingredients or supplements having a functional effect on health treating the effects of consuming alcohol, narcotics or other addictive behavior, e.g. treating hangover or reducing blood alcohol levels
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- Natural Medicines & Medicinal Plants (AREA)
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- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention eosuri (Heracleum The present invention relates to a pharmaceutical composition for the treatment of hangover and liver protection, which comprises an extract of australia fungus HANCE and an extract of moellendorffii HANCE as an active ingredient. More specifically, a 70% fermented spirulina extract is obtained, As a result, it was confirmed that the cell survival rate was increased, and that the aquatic extract increased the activity of the alcoholase, so that the aquatic extract of the present invention can be effectively used as a composition for preventing hangover.
Description
The invention eosuri (Heracleum moellendorffii HANCE) extract as an active ingredient, it is possible to develop related fields such as medicines and foods for improving the inconvenience caused by drinking.
The hanging phenomenon is caused by the toxicity of alcohol (ethyl alcohol) and / or acetaldehyde accumulated in liver cells, and the toxicity of alcohol and / or acetaldehyde is prolonged for a long time to cause a disorder of metabolism and the like, Abdominal bloating, and vomiting.
In the alcohol metabolism process, the alcohol introduced into the body is absorbed from the stomach or small intestine, is transferred into the blood vessels, is transferred to the liver, and the alcohol is dehydrated by alcohol dehydrogenase (ADH) And the acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase (ALDH), an aldehyde degrading enzyme, in hepatocytes, and finally decomposed into carbon dioxide gas and water
On the other hand, type Ⅱ, which initiates oxidation even when acetaldehyde is low in concentration, and type Ⅰ, which does not work if acetaldehyde is not concentrated at a high concentration. However, since Asian people generally lack or lack Ⅱ type aldehyde decomposing enzyme, The oxidation of acetaldehyde and alcohol is slow, and therefore, the toxicity of acetaldehyde and alcohol, which is not oxidized, is interfered with normal metabolism, so that many hangover phenomena occur.
In this regard, much research has been conducted on the effect of reducing the hangover resolution of a composition prepared by using a natural extract alone or in combination. For example, a hangover remedy using green tea (Korean Patent Registration No. 1005604110000), a hangover treatment using a stone tree extract (Korean Patent Registration No. 1012475240000).
On the other hand, eosuri (Heracleum moellendorffii Hance) is a plant of the Umbelliferae, which is a perennial plant that grows relatively frequently in the mountains of Korea. The height of the aquarium is 70 to 150 cm, and the main stem is hollow circumferential type, characterized by thick branches and large hairs. Leaves from the roots and lower leaves have petiole, composed of 3 ~ 5 small lobes, with hairs on the back side and petiole of leaves. The apical lobule is centrifugal and is deeply divided into 3 pieces. The lateral lobes are broad ovate or triangular, divided into 2 ~ 3 pieces and 7 ~ 20 cm long. The lobes have sharp tip and acute sawtooth . Diphtheria inflorescence is run on the tip of the branch and main stem and is divided into 20 ~ 30 sporangiums and runs 25 ~ 30 white flowers respectively. The length of the pedicel is 7 ~ 10cm and the length of the pedicel is not constant. The length of the pedicel is 2m in length and it has hairs on the inside. The fruit is a flat obovate, with a unique pattern near the upper part and no hair (Lee, Changbok, 2003.).
It is said that the root of an aquatic plant is sown in spring and it is planted in spring in October when the leaves are yellowish in July ~ September of the following year. When the leaves are yellowish, they take root and remove stem and root, Muscle aches, arthritis, back pain, and it is used for treatment of itching, swelling, headache, chills and fever.
In the private sector, young leaves of the aquarium are used for food, and fruits and roots are used for various purposes such as feces, cold, beauty, and drainage. However, there is no prior art for the improvement of hangover resolution containing an aquatic extract.
Accordingly, the inventors of the present invention completed the present invention by confirming that aquatic plant extract inhibits cell damage by alcohol treatment and activates an alcohololytic enzyme while studying a composition for hanging up from a natural substance.
An object of the present invention is eosuri (Heracleum The present invention also provides a pharmaceutical composition for the treatment of hangover and liver protection, which comprises an extract of HANCE moellendorffii as an active ingredient.
In order to achieve the above object, the present invention eosuri (Heracleum The present invention provides a pharmacological composition for hangover use containing an extract of Mellendorffii HANCE as an active ingredient.
Further, the present invention provides a health functional food for hangover marine life containing an aquatic extract as an active ingredient.
The present invention also provides a pharmaceutical composition for protecting an alcoholic liver injury containing an aquatic extract as an active ingredient.
In addition, the present invention provides a health functional food for protecting against alcoholic liver damage containing an aquatic extract as an active ingredient.
In addition, the present invention provides a pharmaceutical composition for liver protection containing an aquatic extract as an active ingredient.
Further, the present invention provides a health functional food for liver protection containing an aquatic extract as an active ingredient.
The invention eosuri (Heracleum The present invention relates to a pharmacologically acceptable composition for hangover comprising an extract of mohelendorffii HANCE as an active ingredient, specifically a 70% fermented alcohol extract of aquaculture, which is treated with ethanol to induce cell damage The cell survival rate was confirmed to be increased. The aquatic extract of the present invention can be usefully used as a composition for preventing hangover by confirming that the aquatic extract increases the activity of alcohololytic enzyme.
1 is eosuri (Heracleum Fig. 2 is a view showing a process for preparing a fermented extract from a mellendorffii HANCE.
FIG. 2 is a graph showing the protective effect of an aquatic extract against alcoholic hepatocyte injury.
FIG. 3 is a view showing an alcohol metabolism process. FIG.
Fig. 4 is a graph showing the effect of the alcoholysis enzyme activity of the aquatic extract. Fig.
Hereinafter, the present invention will be described in detail.
The invention eosuri (Heracleum The present invention provides a pharmacological composition for hangover use containing an extract of Mellendorffii HANCE as an active ingredient.
The aquatic extract may be produced by a manufacturing method comprising the following steps.
1) extracting the fish by adding an extraction solvent to the fish;
2) filtering the extract of step 1); And
3) Concentrating the filtered extract of step 2) under reduced pressure and drying.
In the above method, the starling of step 1) can be used without restrictions such as cultivated or commercially available, and leaves, stems or roots are all available,
In this method, any of the conventional methods known in the art can be used as the method of extracting the aquatic extract, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction.
In the above method, the aquatic extract is preferably extracted with water, C 1 to C 2 lower alcohol, alcohol or a mixed solvent thereof. According to a preferred embodiment of the present invention, it is preferable that the aqua extract is 70% fermented.
In the above method, shaking extraction, Soxhlet extraction, or reflux extraction may be used as the extraction method. The extraction solvent may be extracted by adding 1 to 40 times the amount of the dried fish, and the extraction temperature may be 20 to 50 ° C And the extraction time may be 10 to 100 hours, and the extraction frequency may be 1 to 5 times.
In this method, it is possible to use a vacuum decompression concentrator or a vacuum rotary evaporator for the concentration under reduced pressure in step 3). The drying can be carried out under reduced pressure, vacuum drying, boiling, spray drying or freeze drying.
The aquatic extract is characterized in that it increases the cell survival rate by alcohol and increases the activity of alcohololytic enzyme.
In a specific example of the present invention, the present inventors obtained a 70% fermented alcoholic extract of aquatic roots and leaves, treated the aqueous extract with 100 μg / ml of hepatic cell line HepG2, and induced cell damage with 1M ethanol As a result of the measurement of the survival rate, it was confirmed that the cell survival rate was increased when the aquatic leaf extract was treated in advance (see FIG. 2). As a result of confirming the effect of the alcoholysis enzyme activity of the aqua extract, it was confirmed that when the aqua leaf extract was treated at a concentration of 500 μg / ml, the activity of the alcoholase, ADH, was increased (see FIG. 4) It can be usefully used as a pharmacological composition for hangover.
The composition of the present invention may further comprise a pharmaceutically acceptable carrier. The term " pharmaceutically acceptable "as used herein means that the composition is free of toxicity to cells or humans exposed to the composition. Compositions comprising a pharmaceutically acceptable carrier can be of various oral or parenteral formulations. In the case of formulation, it can be prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. The carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, But may be at least one selected from the group consisting of polyvinylpyrrolidone, physiological saline, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol and liquid paraffin, But are not limited to, ordinary carriers, excipients or diluents. The components can be added to the fermented rumba extract as an active ingredient independently or in combination.
Solid formulations for oral administration may include tablet pills, powders, granules, capsules and the like, which may contain one or more excipients, such as starch, calcium carbonate, sucrose or lactose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.
The pharmaceutical composition of the present invention may also be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, Or a pharmaceutically acceptable salt thereof.
The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. There is no particular restriction on the dosage, and it may vary depending on the body's absorption, body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, severity of disease and the like. The pharmaceutical composition of the present invention is prepared in consideration of an effective dose range, and the unit dosage formulations thus formulated are classified according to the judgment of the expert who monitors or observes the administration of the drug, if necessary, Or may be administered several times at a predetermined time interval. Preferably, the composition of the present invention may be administered at a dose of 0.5 to 5000 mg / kg, preferably 50 to 500 mg / kg, more preferably 50 mg / kg, based on the amount of the aquatic extract, The above administration may be carried out once a day or several times.
Further, the present invention provides a health functional food for hangover marine life containing an aquatic extract as an active ingredient.
Preferably, the fish is extracted with a 70% fermented alcoholic beverage.
In a specific example of the present invention, the inventors of the present invention prepared an avian extract and confirmed that the avian extract reduced the cell viability by ethanol in the liver cell line (see FIG. 2) and increased the activity of the alcoholase (See Fig. 4), the aquatic extract of the present invention can be usefully used as a composition for relieving hangover and liver protection and a health functional food.
The health functional food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau Martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate may be selected from the range of 0.01 to 0.04 part by weight, specifically about 0.02 to 0.03 part by weight per 100 parts by weight of the health functional food of the present invention.
In addition to the above, the health functional food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, protective colloid thickener, pH adjuster, stabilizer, preservative, glycerin, A carbonating agent used in a carbonated beverage, and the like. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health food of the present invention.
The present invention also provides a pharmaceutical composition for protecting an alcoholic liver injury containing an aquatic extract as an active ingredient.
In addition, the present invention provides a health functional food for protecting against alcoholic liver damage containing an aquatic extract as an active ingredient.
The aquatic extracts are characterized by increasing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).
The alcoholic liver damage is characterized by acute or chronic alcoholic liver disease (ALD) and alcoholic hepatitis.
In addition, the present invention provides a pharmaceutical composition for liver protection containing an aquatic extract as an active ingredient.
Further, the present invention provides a health functional food for liver protection containing an aquatic extract as an active ingredient.
In a specific example of the present invention, the inventors of the present invention prepared an avian extract and confirmed that the avian extract reduced the cell viability by ethanol in the liver cell line (see FIG. 2) and increased the activity of the alcoholase (See FIG. 4), the aquatic extract of the present invention can be usefully used as a pharmaceutical composition for alcoholic liver damage and liver protection and a health functional food.
Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.
However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.
< Example 1> Preparation of aquaculture extract
Heracleum To prepare the moellendorffii HANCE extract Gangwon-do, Gangwon-do, South Korea, were purchased from Gangneung and were separated into leaves and roots.
Specifically, the algae were removed, and a 70% fermented alcohol was added to 30 g of the aquarium sample 10 times the weight of the sample. The mixture was stirred at room temperature for 16 hours, and filtered to filter paper. To the residue, 70% fermented alcohol was added 10 times and the mixture was stirred at room temperature for 3 hours. The mixture was filtered on filter paper, and then combined with the first extract, concentrated under reduced pressure at 34 ° C, and lyophilized to prepare the final extract.
The extraction yield was 30.79% and the lyophilized weight was calculated as a percentage of the weight of the sample used in the extract preparation.
< Example 2> Cell culture
Hepatocyte HepG2 cells were cultured by the following method in order to confirm the hepatocyte protective effect of aquatic extracts.
Specifically, the cells were cultured in a 5% CO 2 incubator at 37 ° C. in DMEM supplemented with 10% FBS (fetal bovine serum) and 100 IU / ml penicillin (Dulbecco's modified Eagle's medium, Gibco, USA) They were maintained on a quarterly basis.
< Experimental Example 1> Confirmation of hepatocyte protective effect of aquatic extract
The effect of protecting the liver cells of the avian extract prepared in Example 1 was confirmed.
Specifically, the hepatocyte HepG2 cultured by the method of Example 2 was cultured on a plate at a concentration of 7 × 10 3 / well for 24 hours, and then treated with a 100 μg / ml concentration of aquatic herb extracts. After 1 hour, the cells were treated with 1 M of ethanol and cultured for 17 hours to induce cell damage. Cell viability was then measured using the MTS assay method. Specifically, each cell plate well was treated with MTS (Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay) reagent and the degree of color development was measured at 485 nm using an Envision microplate reader (Perkin Elmer). Cell viability was calculated based on the 100% survival rate of the untreated control (control).
As a result, as shown in FIG. 2, the cell survival rate was reduced by 57% as compared with the ethanol-treated cell by treatment with ethanol 1M (FIG. 2). On the other hand, pretreatment of
< Experimental Example 2> Identification of Alcohololytic Activity of Aqua Extract
As shown in FIG. 3, alcohol is decomposed into acetaldehyde by alcohol dehydrogenase (ADH), which is finally decomposed into acetate by aldehyde dehydrogenase (ALDH). The effect of the alcoholysis enzymatic activity of the avian extract prepared in Example 1 was investigated in the following manner.
ADH and ALDH activities were measured by Alcohol Dehydrogenase Activity Colorimetric Assay Kit (Biovision, USA) and Aldehyde Dehydrogenase Activity Colorimetric Assay Kit (Biovision, USA). 100 μl of the agar extract (500 μg / ml) and 100 μl of the reagent (developer, substrate, buffer) were placed in a 96-well plate and reacted at 37 ° C. for 3 minutes. The initial absorbance (A0) was measured at 450 nm. After incubation at 37 ° C for 30 minutes, absorbance (A1) was measured at 450 nm. The absorbance (A1-A0) before and after the reaction was substituted into the standard curve, and the amount of NADH was measured. The ADH activity was measured according to the following equation shown in the kit and expressed as% of the untreated control (control).
[ Equation 1 ]
As a result, as shown in FIG. 4, when the extract of the aquatic herb extract was treated at a concentration of 500 μg / ml, the activity of the alcoholase, ADH, was increased by 20% and the ALDH activity was increased by 30% (FIG.
Claims (8)
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| KR20090003832A (en) * | 2007-07-05 | 2009-01-12 | 조선대학교산학협력단 | A composition comprising the extract of aralia continentalis improving cytotoxicity and liver injury for preventing and treating of liver disease |
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