KR101739827B1 - Compositions containing an extract of phlomis korainensis nakai for anti-aging - Google Patents

Abstract

The anti-aging composition containing the acid-fast extract of the present invention as an active ingredient is excellent in skin cell and collagen biosynthesis promoting effect without inducing skin side effects, and thus can be effectively applied to external preparations for skin anti-aging and wrinkle improvement .

Description

TECHNICAL FIELD [0001] The present invention relates to an anti-aging composition containing an acid-fast extract as an active ingredient. BACKGROUND ART < RTI ID = 0.0 >

The present invention relates to an anti-aging composition comprising an acid-fast extract as an active ingredient.

Skin is the primary barrier of the human body and protects the internal organs of the body from changes in temperature and humidity, from external stimuli such as ultraviolet rays and pollutants, and plays an important role in body homeostasis such as body temperature control. However, excessive physical and chemical stimuli from outside, stress and nutritional deficiency deteriorate the normal function of the skin and promote skin aging phenomenon such as loss of elasticity, keratinization and wrinkle formation. To prevent such phenomenon, There has been an effort to maintain skin's natural functions and activate skin cells to effectively inhibit skin aging by using cosmetics having physiologically active substances obtained from various plants, microorganisms, However, most conventional cosmetic raw materials have various problems such as insufficient efficacy and skin side effects.

It is an object of the present invention to provide a composition for preventing aging of skin which is excellent in promoting skin cell and collagen production without inducing skin side effects by containing an extract of an acid root of a natural plant material as an effective ingredient.

In order to solve the above-mentioned problems, the present invention provides an anti-aging composition containing an acid-fast extract as an active ingredient.

In one embodiment of the present invention, the acidophilic step extract is a mixture of anhydrous and lower alcohol having 1 to 4 carbon atoms, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane , And the like.

In one embodiment of the present invention, the acidophilic step extract may be a primary extraction using at least one of an anhydrous and a lower alcohol having 1 to 4 carbon atoms, a polar solvent containing acetone and butylene glycol, And may be extracted through a secondary extraction using one or more of low-polarity solvents including ethyl acetate, diethyl ether, benzene, chloroform and hexane.

In one embodiment of the present invention, the content of the acid end extract may be 0.0001 to 10% by weight based on the total weight of the composition.

In one embodiment of the present invention, the anti-aging composition may have a use for improving skin wrinkles.

In one embodiment of the present invention, the anti-aging composition may be one having an application for skin elasticity enhancement.

In one embodiment of the present invention, the anti-aging composition may prevent aging through proliferation of skin cells or fibroblasts.

In one embodiment of the present invention, the anti-aging composition may prevent aging by promoting collagen biosynthesis.

The anti-aging composition containing the acid-fast extract of the present invention is excellent in promoting skin cell and collagen biosynthesis without inducing skin side effects. Due to these effects, it can be usefully applied to external preparations for skin intended for anti-aging and wrinkle improvement.

Hereinafter, preferred embodiments of the present invention will be described in detail in order to facilitate the present invention by those skilled in the art.

The present invention provides an anti-aging composition comprising an acid-fast extract as an active ingredient.

The mountain is a perennial plant of the family Lamiaceae, and its scientific name is Phlomis Korainensis Nakai. It grows in a deep mountain and has a height of about 60㎝. Its leaves are opposite to each other, and flowers bloom from August to September. The roots grow fusiform and spread all over the place. The mountain range is a Korean species and is distributed in South Hamgyong, Hamgyung, North Pyongan, and Pyongan. In the civilian area, the roots of the mountain range were called as the local roads, and they were used for bruises and women's diseases. However, the wrinkle improvement and anti-aging efficacy of the mountain clade have not been studied to date.

In the present invention, it is preferable to use roots at the end of the acid range. The acid-fast extract of the present invention is obtained by drying and crushing the roots of the acid end; The pulverized product obtained is mixed with at least one extraction solvent selected from the group consisting of anhydrous and lower alcohol having 1 to 4 carbon atoms, acetone, butylene glycol, ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane Extract more than once by cold or warming; The extract thus obtained can be prepared by concentrating the extract under reduced pressure to a suitable temperature in a distillation apparatus equipped with a cooling condenser.

More preferably, the extraction step is firstly subjected to primary extraction using at least one of an anhydrous and a lower alcohol having 1 to 4 carbon atoms, a polar solvent containing acetone and butylene glycol, and then sequentially extracted with ethyl acetate, diethyl Or a secondary extraction using one or more of low-polarity solvents including acetone, diethyl ether, benzene, chloroform and hexane. More specifically, as an extraction solvent, water, anhydrous and lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, n-propanol, n-butanol), a mixture of water and lower alcohol, acetone, 1,3-butylene glycol and isopropanol The polar solvent and the mixed solvent thereof are precipitated by adding 5 to 20 times the volume of the dry weight of the ground powder of Chungho-mugwort, and filtered. The filtrate is concentrated under reduced pressure. Water is added to the obtained concentrate and dispersed. To the dispersion is added a low-polarity organic solvent containing the same amount of ethyl acetate, diethyl acetate, diethyl ether, benzene, chloroform and hexane and shaken. Thereafter, the organic layer is separated and concentrated under reduced pressure to obtain a crude wormwood extract.

The extraction may be carried out for about 12 to 96 hours in the case of cold beating, or by aging at room temperature for 3 to 20 days at a temperature of 4 to 25 DEG C with an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or diethyl ether as a solvent, The active ingredient can be extracted. In the case of warming, it is preferable that the heating is carried out at a temperature close to the reflux temperature of the solvent for about 5 to 24 hours although it depends on the kind and temperature of the extraction solvent.

By the extraction method which sequentially extracts according to the polarity change as described above, the content of the active ingredient is maximized and the impurity is removed to improve the stability, and the extract having the maximized effect can be obtained.

It is preferable that the acid end extract is contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition. When the acid extract is contained in an amount of less than 0.0001% by weight based on the total weight of the composition, the effect of improving wrinkles and anti-aging is insignificant. When the amount of the extract is more than 10% by weight,

The anti-aging composition according to the present invention may be, for example, a pharmaceutical composition or a cosmetic composition.

The anti-aging pharmaceutical composition may further contain a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure, and other therapeutically useful substances, Can be formulated in a variety of parenteral dosage forms. Parenteral dosage forms, and may be, for example, but not limited to lotions, ointments, gels, creams, patches or spray formulations.

The dosage of the active ingredient is within the level of those skilled in the art, and the daily dose of the drug depends on various factors such as the degree of progress of the subject to be administered, the age of onset, age, health condition, As a standard, it is generally possible to administer the composition at a dose of 1 / / kg to 200 mg / kg, preferably 50 / / kg to 50 mg / kg, once or twice a day, Are not intended to limit the scope of the present invention.

The formulation for the anti-aging cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milky lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing But the present invention is not limited thereto, and may be manufactured by any one or more formulations selected from the group consisting of a foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The content of the active ingredient is not particularly limited, but may be in the range of 0.0001 to 10% by weight based on the total weight of the composition. When the active ingredient satisfies the above content, it can exhibit excellent efficacy without side effects.

The cosmetic composition may further contain ingredients included in the functional additive and the general cosmetic composition in addition to the acid starch extract prepared as described above. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The cosmetic composition of the present invention may further contain, in addition to the above-described functional additive, components contained in a general cosmetic composition as required. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

Further, the present invention relates to an external preparation for skin comprising the anti-aging composition, wherein the external preparation for skin is a generic term that may be applied to any external skin, and cosmetics and pharmaceuticals of various formulations may be included. The external preparation for skin is not particularly limited, but may be, for example, a skin external preparation for preventing skin aging or improving skin wrinkles.

The present invention has found an excellent skin anti-aging effect by using a composition containing an acid starch extract as a natural raw material. The composition for anti-aging according to the present invention has a function of promoting skin cell and collagen biosynthesis, as can be seen in a test example to be described later, so that the wrinkles of the skin can be improved.

The present invention will be described in more detail through the following examples. However, the examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.

[ Example  One] Mountain range  Preparation of extract

The roots of the mountain clusters were washed with purified water, dried and then chased. 200 g of palm root powder in the hermaphroditic acid was added to 1 liter of a 70% aqueous ethanol solution, which was boiled for 12 hours in an extractor equipped with a condenser and filtered through a 300-mesh filter cloth. The filtrate was allowed to stand at 4-15 ° C for 7 days and then aged. Then, the filtrate was filtered through Wattman filter paper No. 2. Then, the extract solution was added to a 3 liter separatory funnel, 1 liter of ethyl acetate was added thereto, and the mixture was shaken and mixed thoroughly. After separating completely into two layers, an upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was further extracted twice with a separatory funnel. The separated upper layers were combined and concentrated under reduced pressure at 50 ° C using a distillation apparatus equipped with a cooling condenser and dried to obtain 25.5 g of a dry starch extract.

[ Test Example  1] Wrinkle improvement effect

The test for measuring the wrinkle-improving effect of the acid-fast extract of Example 1 was conducted. As a wrinkle-improving effect, in order to examine skin cell proliferation effect, human normal fibroblasts were inoculated into each well of a 96-well microplate so as to have a density of 1 × 10 ⁴ cells, And cultured in DMEM (Dulbecco's Modified Eagle's Medium) for 24 hours. After culturing, the acid-fast extract prepared in Example 1 was replaced with serum-free DMEM medium adjusted to a final concentration of 500 μg / ml, and further cultured for 24 hours. 3- (4,5-Dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium (MTT: 5 mg / ml) was added to each well and allowed to stand for 4 hours, after which the medium was discarded. 100 μl of dimethyl sulfoxide solution was added to each well and the absorbance was measured at 570 nm with a microplate reader.

The above process was tested to determine the cell proliferation effect from the following equation (1), and the results are shown in Table 1 below.

[Equation 1]

Cell proliferation effect (%) =

Wrinkle improvement effect Acid starch extract concentration (/ / ml) Absorbance (570 nm) Cell proliferation effect (%) 0 (control group) 0.300 - 500 (Example 1) 0.420 40.0

As shown in the above Table 1, it was found that the acid-fast extract had cell proliferation effect of normal fibroblasts.

[ Test Example  2] Promoting collagen biosynthesis

A test for measuring the promoting effect of collagen biosynthesis of the acid-fast extract of Example 1 was conducted. In order to examine the effect of promoting collagen biosynthesis as a wrinkle-improving effect, human normal fibroblasts were inoculated into each well of a 96-well microplate so as to have a concentration of 1 × 10 ⁴ cells, (DMEM: Dulbecco's Modified Eagle's Medium) medium for 24 hours. After culturing, the acid-fast extract prepared in Example 1 was replaced with serum-free DM medium adjusted to a final concentration of 500 μg / ml, and then cultured for another 48 hours. 50 ug / ml of ascorbic acid is added 24 hours prior to incubation to promote collagen synthesis. After incubation, each well is washed and replaced with DMEM medium containing no serum, followed by further culture for 24 hours. After the incubation, the supernatant of each well was collected and the amount of collagen type IC-peptide (PICP, Type-I C-peptide) was measured using a kit (Kit, Takara, Kyoto, Japan) , And the synthesis effect of collagen in terms of the amount of PICP in terms of ng / 2 × 10 ⁴ cells is shown in Table 2 below.

Promoting collagen biosynthesis Acid starch extract concentration (/ / ml) Collagen synthesis amount (ng / 2 × 10 ⁴ ) 0 (control group) 140 500 (Example 1) 200

As shown in the above Table 2, the amount of collagen synthesis in the acidophilus extract was increased as compared with that of the control. Thus, it was found that the acidophilus extract promotes collagen biosynthesis.

Formulation examples of the composition containing the acid-fast extract of the present invention are described below, but the composition of the present invention is not limited to this example.

[ Formulation Example  1] lotion type formulation

Acid starch extract of Example 1 3.00

L-ascorbic acid-2-phosphate magnesium salt 1.00

Water soluble collagen (1% aqueous solution) 1.00

Sodium citrate 0.10

Citric acid 0.05

Licorice extract 0.20

1,3-butylene glycol 3.00

Purified water balance

(Unit: wt%)

[ Formulation Example  2] Cream type  Formulation

Acid starch extract of Example 1 1.00

Polyethylene glycol monostearate 2.00

Self emulsifying monostearate glycerin 5.00

Cetyl alcohol 4.00

Squalane 6.00

Tri-2-ethylhexane glyceryl 6.00

Sphingo glycolipid 1.00

1,3-butylene glycol 7.00

Purified water balance

(Unit: wt%)

[ Formulation Example  3] Pack type  Formulation

Acid starch extract of Example 1 5.00

Polyvinyl alcohol 13.00

L-ascorbic acid-2-phosphate magnesium salt 1.00

Lauroylhydroxyproline 1.00

Water soluble collagen (1% aqueous solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water balance

(Unit: wt%)

[ Formulation Example  4] Beauty liquid  Formulation

Acid starch extract of Example 1 2.00

Hydroxyethylene Cellulose (2% aqueous solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark glycerin 4.00

Sodium hyaluronate (1% aqueous solution) 5.00

Purified water balance

(Unit: wt%)

Claims (8)

( Phlomis Korainensis Nakai ) as an active ingredient, and the acid end extract is extracted through secondary extraction using ethyl acetate in a primary extract using ethanol. Wherein the extract of Phytophthora ( Phlomis Korainensis Nakai ) is an active ingredient, and the extract of the acidophilus is extracted through secondary extraction using ethanol as a primary extract with ethyl acetate. delete 3. The method according to claim 1 or 2,
Wherein the content of the acid end extract is 0.0001 to 10% by weight based on the total weight of the composition. delete delete 3. The method according to claim 1 or 2,
Wherein the composition enhances skin wrinkles or skin elasticity through proliferation of skin cells or fibroblasts. 3. The method according to claim 1 or 2,
Wherein said composition promotes skin wrinkles or skin elasticity through promoting collagen biosynthesis.