KR101732720B1 - A composition for anti-tussive activity or discharge of phlegm activity comprising of lonicera japonica, scutellaria baicalensis and liriopis tuber - Google Patents

Abstract

The present invention relates to a composition for deep sea or dandruff containing, as an active ingredient,
More particularly, the present invention relates to a process for producing a fermented soybean oil, which comprises 1 to 5 parts by weight of potash, 0.5 to 1.5 parts by weight of gold and 0.5 to 1.5 parts by weight of iron powder and extracted with ethanol containing 3% And a mixed extract of ginseng root as an active ingredient.
The composition according to the present invention has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a composition for a deep sea or a dandruff containing an extract of gold and mulberry leaves as an active ingredient,

The present invention relates to a composition for deep sea or dandruff containing, as an active ingredient,

More particularly, the present invention relates to a process for producing a fermented soybean oil, which is extracted with 1 to 5 parts by weight of potato, 0.5 to 1.5 parts by weight of gold and 0.5 to 1.5 parts by weight of corn sweet potato, using ethanol containing 0.5 to 5% , A mixture of gold and ginseng root as an active ingredient.

The composition according to the present invention has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

The respiratory system of our body is one of the defense institutions against the physicochemical external stimuli, and the respiratory disease patients are rapidly increasing due to the air pollution and environmental change due to rapid industrialization.

When the external stimulant enters the respiratory tract, it is primarily filtered by the nostrils, and the micro-stimulant that has passed through it is captured by the mucus and released through the ciliary movement.

Coughs are caused by mucous membrane stimulation and excessive coughing by excessive irritation or continuous irritation before the reflexive defense mechanism lowers the quality of life of the patient and accelerates the damage of the bronchial tubes.

In addition, in the case of sputum, exogenous substances are released to the outside together with saliva or mucus material, and purulent sputum is generated by bronchial inflammation.

Among the medicines used for these symptoms, dihydrocodeine, methylphedrine, chlorophenylamine, and dextromethorphan act on the central nervous system.

These components exhibit side effects such as myocardial infarction, respiratory depression, stomach or intestinal bleeding due to cardiovascular thrombosis.

Chrysanthemums such as acetylcysteine, eldostaine, carbostein, etc., are used indiscriminately in the case of insecticides.

Therefore, much research has been conducted to develop a broad-leavening expectorant using safer natural materials. In the present invention, as a result of confirming the activity of shinhae / Completed.

It is also known as Lonicera leaf and stem. It is also known as Lonicera Japonica Thunberg, which belongs to the genus Caprifoliaceae. , Urine discomfort, and muscle pain.

Golden (黃 芩) is a perennial plant belonging to the family Lamiaceae ( Scutellaria baicalensis Georgi) has the efficacy as the roots of cheonggyu (淸 热 燥 濕) and poisoning (泻火 解毒).

Liriopis tuber is the bulge of the root of Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler, a perennial herb belonging to Liliaceae.

The ingredients include steroid saponin, isoflavonoid, oligosaccharides and polysaccharide.

McMundong has been reported to have hypoglycemic, anti-inflammatory, and anti-IgM antibody production.

On the other hand, as a prior art related to the present invention, Patent Publication No. 10-1367803 discloses a pharmaceutical composition containing mahwang for treating bronchitis and a manufacturing method of the same.

The pharmaceutical composition of the present invention is a composition which alleviates coughing and alleviates coughing, and is effective for preventing cough, , Asthma is calmed down, and there is an effect useful in preventing inflammation.

However, the prior art has a problem in that the effect of mixed extraction of the material is unknown because the material is extracted and mixed after each extraction.

In addition, since the above-mentioned prior art extracts ethanol from Mahwang, Kwanghwang, Kwangyang, Golden, passerby, Vanilla, Wisteria and Rhubarb, and extracts only ginseng with gypsum, The efficacy of the extract on bronchitis is unknown.

Another prior art related to the present invention is disclosed in Korean Patent Registration No. 10-0566542, which discloses a health food composition for the prevention and treatment of allergic rhinitis and cold and a method for producing the same.

The above-mentioned prior arts include symptoms of allergic rhinitis including active ingredients such as rhizomes, dandruff, licorice, omija, hwanggi, It is characterized in that it has an excellent therapeutic effect in improvement.

However, since the above-described prior art only observes clinical symptoms for allergic patients, it has not been able to objectively evaluate the efficacy of the composition on dandruff activity or vigorous activity because the experiment is not conducted through the proven method.

In addition, since the prior art is extracted using only the hot water extraction method, there is a problem that the efficacy of the extract according to the type of the extraction solvent is unknown.

Korean Registered Patent No. 10-1367803 (Feb. Korean Registered Patent No. 10-0566542 (Mar. 24, 2006)

A problem to be solved by the present invention is to provide a composition for deep sea or dandruff containing, as an active ingredient, a mixed extract of golden and mulberry leaves, such as indigo.

The problem to be solved by the present invention is to provide a process for producing a fermented soybean milk, which comprises mixing 1 to 5 parts by weight of potato, 0.5 to 1.5 parts by weight of gold and 0.5 to 1.5 parts by weight of corn germ oil, and extracting using ethanol containing 0.5 to 5% , Rhizome, and the like, and a mixed extract of gold and marshmallow as an active ingredient.

A problem to be solved by the present invention is to provide a composition for deep sea or dwarf, which has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

A problem to be solved by the present invention is to provide a composition for deep sea or dandruff containing, as an active ingredient, a mixed extract of golden and mulberry leaves, such as indigo.

The problem to be solved by the present invention is to provide a process for producing a fermented soybean milk, which comprises mixing 1 to 5 parts by weight of potato, 0.5 to 1.5 parts by weight of gold and 0.5 to 1.5 parts by weight of corn germ oil, and extracting using ethanol containing 0.5 to 5% , Rhizome, and the like, and a mixed extract of gold and marshmallow as an active ingredient.

A problem to be solved by the present invention is to provide a composition for deep sea or dwarf, which has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

The composition for deep sea or dandruff containing the mixed extract of golden and mulberry leaves as an active ingredient according to the present invention has an excellent efficacy against sputum.

The composition for deep sea or dandruff containing the mixed extract of golden and mulberry leaves as an active ingredient according to the present invention has an excellent effect of inhibiting cough.

The composition for deep sea or dandruff containing the mixed extract of golden and mulberry leaves as an active ingredient according to the present invention has an excellent antioxidant activity.

FIG. 1 shows the sputum-releasing activity of the mixed extract, which is a graph measuring the concentration of the released phenol.
FIG. 2 is a graph showing the cough suppressing ability of the mixed extract, which is a measurement of the number of coughs occurring for 5 seconds.
FIG. 3 is a graph showing the antioxidative effect of the mixed extracts, and is a graph showing the absorbance in terms of%.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the experimental examples and the reference examples described in the present specification are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents and variations Examples should be understood.

Example  One. Incandescent light , Golden and Mantum Dong mixed extract ( HAE -03)

And rhododendron can be mixed and extracted at 1 to 5: 0.5 to 1.5: 0.5 to 1.5 parts by weight. Preferably 2.5 to 3.5: 1: 1, by weight.

Various extraction methods such as a carbon dioxide supercritical extraction method, a steam distillation method, a vacuum extraction method, a vacuum extraction method, a microwave process extraction method, and a percolation extraction method may be used, but a solvent extraction method may be preferably used.

The solvent used for the extraction is selected from the group consisting of water, ethanol, methanol, fatty oil, glycerin, hemp, flouropylene glycol, ether, chloroform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol, isopropanol, Antimony, hydrogen fluoride and the like.

As a preferred extraction method, the materials are mixed, and then 10% aqueous solution of 30% (v / v) ethanol is added thereto, refluxed at 80 to 90 ° C for 3 hours and then filtered to obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method, and then the extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain a dried extract, whereby a golden and mulberry root mixed extract can be prepared.

Example  2. Incandescent light , Golden and Mantum Dong mixed extract ( HAE -04)

(V / v) aqueous solution of ethanol containing 3% (v / v) acetic acid in 30% (v / v) do.

In order to evaluate the efficacy of the golden and manganese mixed extracts such as potatoes according to Examples 1 and 2, the extracts of golden and Gyungyang were prepared and compared together.

Comparative Example  One. Incandescent light , Gold and Gakyung  Mixed extract ( HAE -01)

40 g of gold and 40 g of gypsum and put in 10 volume of 30% (v / v) ethanol solution, reflux cooled for 3 hours at 80 ~ 90 ℃ and then filtered to obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method. The extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain about 57.6 g (yield: 28.80%) of dried extract.

Comparative Example  2. Incandescent light , Gold and Gakyung  Mixed extract ( HAE -02)

(V / v) aqueous ethanol solution containing 3% (v / v) acetic acid was added to 10 g of water, reflux cooled for 3 hours at 80 to 90 ° C., To obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method. The extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain about 53.5 g (yield: 26.75%) of dried extract.

Experimental Example  1. Evaluation of gadam activity

1-1. Experimental course

(Engler H, Szelenyi I, J. Pharmacol. Moth., 151 ~ 157, 1984, Bao-quin Lin et al., Pulmonary Pharmacology & Therapeutics 21, 259-263, 2008.) Evaluation experiments were conducted. The experimental procedure is as follows.

The test compound was orally administered to male rats (6 weeks old, Sumatoco Bio Korea) for 30 minutes, and the test compound was intraperitoneally injected with phenol red (200 mg / kg dose) And phenol red dissolved in physiological saline).

② After 30 minutes, anesthesia was performed with diethyl ether, and the abdominal aorta was cut to bleed, and then the entire trachea was excised.

③ Separated organs were washed in 1 ml of physiological saline for 30 minutes and centrifuged at 10,000 rpm for 5 minutes at room temperature. 1 N sodium hydroxide (NaOH) was added to the supernatant (0.1 ml of 1N NaOH per 1 ml of supernatant ).

(4) The absorbance at 546 nm was measured to evaluate the drought activity as the concentration of the phenol red discharged.

1-2. Experiment result

Table 1 below is a table showing the sputum-releasing ability according to the administration dose of ambroxol used as a positive control drug and the mixed extract prepared.

division Dosage (mg / kg) Sputum Emission Rate (%) HAE-01 200 17 HAE-02 200 30 HAE-03 200 22 HAE-04 200 66 Ambroxol 250 91

The results showed that the ethanol extract (HAE-03) of golden and mangmundong ethanol extracts (Hae-gyeong) had a higher sputum emission ability than the ethanol extract of Haengdong et al. (HAE-01) (HAE-04) of acetic acid in golden and macromuscular ginsengs, such as potash, is higher than that of ethanol-containing ethanol extract (HAE-02).

Therefore, it was found that the extracts of gold and mangmundong combination extracts of sponges were more excellent in sputum production ability than extracts of the same method and solvent.

In addition, ethanol extracts containing 3% acetic acid showed better sputum emission than ethanol extracts.

FIG. 1 shows the sputum-releasing activity of the mixed extract, which is a graph measuring the concentration of the released phenol.

As a result, it was confirmed that the concentration of amoxicillin phenol in the positive control group was about twice that of the control group.

In the HAE-01 to HAE-04 experimental groups, the phenol concentration was increased compared to the control group, and it was found that they had sputum-releasing ability. Especially HAE-04 test group (3% Of the patients were found to have significant sputum production.

Experimental Example  2. Evaluation of Chinhae activity

2-1. Experimental course

In order to measure the activity of the mixed extract, Tanaka et al. (Motomu Tanaka and Kei Maruyama, J. Pharmacol. Sci., 93, 465-470, 2003. Daoui, Cognon, Naline et. J. Respir. Crit Care, Med., 158, 42-48, 1998.). The experimental procedure is as follows.

After 30 minutes of oral administration of the drug to a male ICR mouse (6 weeks old, Orient Bio), the mice were placed in a plexi-smear chamber (Buxco, USA) and cough induced by spraying a cough inducer (citric acid).

Theobromine (50 mg / kg) was used as a positive control, and mice were exposed to 0.4 M citric acid for 10 minutes, and the cough water generated was measured at intervals of 5 seconds for 10 minutes.

2-2. Experiment result

Table 2 below is a table showing cough inhibition according to the dose of theobromine used as a positive control drug and the mixed extract prepared.

division Dosage (mg / kg) Coughing / 5 seconds Control group - 2.44 ± 0.24 HAE-01 200 2.55 + - 0.33 HAE-02 200 2.67 ± 0.34 HAE-03 200 2.47 ± 0.28 HAE-04 200 2.53 + - 0.34 Theobromine 50 2.31 ± 0.17

As a result, the number of coughs in the HAE-03 and HAE-04 test groups was reduced compared to the HAE-01 and HAE-02 test groups.

FIG. 2 is a graph showing the cough suppressing ability of the mixed extract, which is a measurement of the number of coughs occurring for 5 seconds.

As shown in FIG. 2, it was confirmed that the number of coughs decreased in the theobromine-treated group compared to the control group.

In addition, it was confirmed that the number of coughs was further reduced by the mixed extract of golden and mackerel dung, such as indigo, compared with the mixed extract of gold and gyeongyang, such as indigo.

Experimental Example  3. Antioxidant activity evaluation

3-1. Experimental course

The DPPH assay is one of the most common experiments to measure antioxidant efficacy. The principle of DPPH assay is that the deep purple Diphenylpicrylhydrazyl is reduced to Diphenylpicrylhydrazine by the antioxidant and turns into a pale yellow. The stronger the antioxidant power of the antioxidant, the closer it is to the pale yellow.

Also, when measuring the absorbance, a dark purple color is detected but a light yellow color is not detected. Therefore, the smaller the absorbance value is, the higher the antioxidant power is. The experimental procedure is as follows.

① 8 groups of 0.1 mM DPPH solution (Diphenylpicrylhydrazyl) were prepared.

② Group 1 was set as negative control and no manipulation was performed. Group 2 was set as positive control and treated with ascorbic acid (Vitamin C) 50M.

③ In the experiment group, the extracts were treated with 10 g / mL, 30 g / mL and 100 g / mL, respectively.

④ We observed how the color of the samples changed using a light absorber at a wavelength of 520 nm.

⑤ The measured absorbance was converted in% by the following equation.

3-2. Experiment result

The following Table 3 shows the antioxidative effect of the mixed extracts. The antioxidative effect of the mixed extracts is shown in Table 3, and FIG. 3 is a graph showing the antioxidative effect of the mixed extracts.

division Treatment volume ([mu] g / ml) Antioxidative effect(%)
HAE-01
10 12.1 30 69.2 100 48.8
HAE-02
10 11.2 30 72.1 100 70.3
HAE-03
10 9.8 30 18.3 100 69.4
HAE-04
10 17.3 30 72.2 100 72.5 Vitamin C 50 μM 76.3

As a result of the experiment, all of the mixed extracts showed a concentration-dependent antioxidative effect as compared with the negative control group.

Claims (5)

Which comprises as an active ingredient, a mixed extract of gold and rhododendron,
The above-
0.5 to 1.5 parts by weight of gold and 0.5 to 1.5 parts by weight of copper powder,
By using ethanol containing 0.5 to 5% of ethanol or acetic acid as a solvent,
Wherein the extract has a sputum-releasing ability, a cough suppressing ability, and an antioxidant activity, as an active ingredient.
delete delete delete Which comprises as an active ingredient a mixed extract of golden and mulberry leaves.

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