KR101619733B1 - A composition for anti-tussive activity or discharge of phlegm activity comprising of lonicera japonica, scutellaria baicalensis, adenophorea radix and polygonati odorati - Google Patents

Abstract

The present invention relates to a composition for deep sea or dandruff containing an extractive mixture of golden, quartz and jade, as an active ingredient.
More specifically, 5 to 10 parts by weight of potash, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of jade are mixed with ethanol containing 3% of ethanol or acetic acid as a solvent The present invention relates to a composition for a deep sea or a dwarf, which contains, as an active ingredient,
The composition according to the present invention has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

Description

FIELD OF THE INVENTION The present invention relates to a composition for a deep sea or a fungus containing an extract of gold, quartz and jade mixed extracts as an active ingredient. The present invention relates to a composition for a deep sea or a dandruff,

The present invention relates to a composition for deep sea or dandruff containing an extractive mixture of golden, quartz and jade, as an active ingredient.

More specifically, 5 to 10 parts by weight of phosphorus, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of jade are mixed with ethanol containing 0.5 to 5% of ethanol or acetic acid as a solvent The present invention also relates to a composition for deep sea or dwarf, which contains, as an active ingredient,

The composition according to the present invention has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

The respiratory system of our body is one of the defense institutions against the physicochemical external stimuli, and the respiratory disease patients are rapidly increasing due to the air pollution and the environmental change due to the rapid industrialization.

When the external stimulant enters the respiratory tract, it is primarily filtered by the nostrils, and the micro-stimulant that has passed through it is captured by the mucus and released through the ciliary movement.

Coughs are caused by mucous membrane stimulation and excessive coughing by excessive irritation or continuous irritation before the reflexive defense mechanism lowers the quality of life of the patient and accelerates the damage of the bronchial tubes.

In addition, in the case of sputum, exogenous substances are released to the outside together with saliva or mucus material, and purulent sputum is generated by bronchial inflammation.

Among the medicines used for these symptoms, dihydrocodeine, methylphedrine, chlorophenylamine, and dextromethorphan act on the central nervous system.

These components exhibit side effects such as myocardial infarction, respiratory depression, stomach or intestinal bleeding due to cardiovascular thrombosis.

Chrysanthemums such as acetylcysteine, eldostaine, carbostein, etc., are used indiscriminately in the case of insecticides.

Therefore, in the present invention, the activity of improving the symptom is confirmed by confirming the activity of Jinhae / Gadam in the sea bream, gold, quartz and jade, Thereby completing the invention.

It is also known as Lonicera leaf and stem. It is also known as Lonicera Japonica Thunberg, which belongs to the genus Caprifoliaceae. , Urine discomfort, and muscle pain.

Golden (黃 芩) is a perennial plant belonging to the family Lamiaceae ( Scutellaria baicalensis Georgi) has the efficacy as the roots of cheonggyu (淸 热 燥 濕) and poisoning (泻火 解毒).

Adenophorae Radix is the root of Adenophora triphylla var. Japonica Hara or Adenophora stricta Miq. (Campanulaceae).

The known components of the ginseng are Taraxerone, Carotene Daucostrol, Octacosanomic acid, phospholipids, phosphatidic acid, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl glycerol, phosphatidyl inositol and Diphosphatidyl glycerol.

Sadam has traditionally been used for genital warts, bronchitis, asthma, pneumonia, fever, and urinary incontinence. Recent studies have shown that water extracts from the sea have an anti-inflammatory and anti-asthmatic effect (Roh SS, Kim SH, Lee YC, Seo YB, Mediators Inflamm. 2008).

Polygonati Odorati Rhizoma is a perennial plant belonging to Liliaceae, Polygonatum there is Odoratum Druce. pluriflorum Ohwi) or other related plants in the spring and autumn.

Ingredients include convallamarin, convallarin, and vitamin A in the rhizome, containing 25.6 ~ 30.6% of starch and mucilage.

It has been reported that it is effective for the effects of noxious dead nourishment, diabetes, noise, weakness, malnutrition, pulmonary tuberculosis, arteriosclerosis, lowering blood pressure and controlling blood glucose.

On the other hand, as a prior art related to the present invention, Patent Publication No. 10-1367803 discloses a pharmaceutical composition containing mahwang for treating bronchitis and a manufacturing method of the same.

The pharmaceutical composition of the present invention is a composition which alleviates coughing and alleviates coughing, and is effective for preventing cough, , Asthma is calmed down, and there is an effect useful in preventing inflammation.

However, the prior art has a problem in that the effect of mixed extraction of the material is unknown because the material is extracted and mixed after each extraction.

In addition, since the above-mentioned prior art extracts ethanol from Mahwang, Kwanghwang, Kwangyang, Golden, passerby, Vanilla, Wisteria and Rhubarb, and extracts only ginseng with gypsum, The efficacy of the extract on bronchitis is unknown.

Another prior art related to the present invention is disclosed in Korean Patent Registration No. 10-0566542, which discloses a health food composition for the prevention and treatment of allergic rhinitis and cold, and a method for producing the same.

The above-mentioned prior arts include symptoms of allergic rhinitis including active ingredients such as rhizomes, dandruff, licorice, omija, hwanggi, It is characterized in that it has an excellent therapeutic effect in improvement.

However, since the above-described prior art only observes clinical symptoms for allergic patients, it is not possible to objectively evaluate the efficacy of the composition on dandruff activity or vigorous activity, etc., since the experiment was not conducted through the proven method.

In addition, since the prior art is extracted using only the hot water extraction method, there is a problem that the efficacy of the extract according to the type of the extraction solvent is unknown.

Korean Registered Patent No. 10-1367803 (Feb. Korean Registered Patent No. 10-0566542 (Mar. 24, 2006)

A problem to be solved by the present invention is to provide a composition for deep sea or dandruff containing, as an active ingredient, a mixed extract of gold, quartz and jade, such as indigo.

A problem to be solved by the present invention is to provide a process for producing an aqueous solution containing 5 to 10 parts by weight of phosphorus, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of corundum, Which comprises extracting a mixture of extracts of indigo, gold, quartz and jade, which are extracted using a solvent as a solvent, as an active ingredient.

A problem to be solved by the present invention is to provide a composition for deep sea or dwarf, which has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

A problem to be solved by the present invention is to provide a composition for deep sea or dandruff containing, as an active ingredient, a mixed extract of gold, quartz and jade, such as indigo.

A problem to be solved by the present invention is to provide a process for producing an aqueous solution containing 5 to 10 parts by weight of phosphorus, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of corundum, Which comprises extracting a mixture of extracts of indigo, gold, quartz and jade, which are extracted using a solvent as a solvent, as an active ingredient.

A problem to be solved by the present invention is to provide a composition for deep sea or dwarf, which has sputum-releasing ability, cough suppressing ability and antioxidant efficacy.

The composition for deep sea or dandruff containing the mixed extract of gold, quartz and jade, as the active ingredient, according to the present invention has excellent efficacy on sputum emission.

The composition for deep sea or dandruff containing the mixed extract of gold, quartz and jade, as an effective ingredient, according to the present invention has an excellent effect of inhibiting cough.

The composition for deep sea or dandruff containing the mixed extract of gold, quartz and jade, as an effective ingredient, according to the present invention has an excellent antioxidant activity.

FIG. 1 shows the sputum-releasing activity of the mixed extract, which is a graph measuring the concentration of the released phenol.
FIG. 2 is a graph showing the cough suppressing ability of the mixed extract, which is a measurement of the number of coughs occurring for 5 seconds.
FIG. 3 is a graph showing the antioxidative effect of the mixed extracts, and is a graph showing the absorbance in terms of%.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the experimental examples and the reference examples described in the present specification are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents and variations Examples should be understood.

Example  One. Incandescent light , Gold, Sasayam  And Jade  Mixed extract ( HAE -05)

5 to 10: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5 parts by weight of gold, silver and rhododendrons. Preferably 7 to 9: 1: 1: 1.

Various extraction methods such as a carbon dioxide supercritical extraction method, a steam distillation method, a vacuum extraction method, a vacuum extraction method, a microwave process extraction method, and a percolation extraction method may be used, but a solvent extraction method may be preferably used.

The solvent used for the extraction is selected from the group consisting of water, ethanol, methanol, fatty oil, glycerin, hemp, flouropylene glycol, ether, chloroform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol, isopropanol, Antimony, hydrogen fluoride and the like.

As a preferred extraction method, the materials are mixed, and then 10% aqueous solution of 30% (v / v) ethanol is added thereto, refluxed at 80 to 90 ° C for 3 hours and then filtered to obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method, and then the extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain dried extract to produce a mixture of golden, quartz and jade mixed extracts.

Example  2. Incandescent light , Gold, Sasayam  And Jade  Mixed extract ( HAE -06)

(V / v) aqueous solution of ethanol containing 3% (v / v) acetic acid in 30% (v / v) do.

In order to evaluate the efficacy of the mixed extracts of golden, quartz and jade, according to Examples 1 and 2, mixed extracts of golden and Gyungyang were prepared and compared together.

Comparative Example  One. Incandescent light , Gold and Gakyung  Mixed extract ( HAE -01)

40 g of gold and 40 g of gypsum and put in 10 volume of 30% (v / v) ethanol solution, reflux cooled for 3 hours at 80 ~ 90 ℃ and then filtered to obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method. The extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain about 57.6 g (yield: 28.80%) of dried extract.

Comparative Example  2. Incandescent light , Gold and Gakyung  Mixed extract ( HAE -02)

(V / v) aqueous ethanol solution containing 3% (v / v) acetic acid was added to 10 g of water, reflux cooled for 3 hours at 80 to 90 ° C., To obtain a primary extract.

The residue is repeatedly extracted in the same manner as in the first extraction method. The extract is concentrated under reduced pressure at 60 ° C or lower and vacuum dried to obtain about 53.5 g (yield: 26.75%) of dried extract.

Experimental Example  1. Evaluation of gadam activity

1-1. Experimental course

(Engler H, Szelenyi I, J. Pharmacol. Moth., 151 ~ 157, 1984, Bao-quin Lin et al., Pulmonary Pharmacology & Therapeutics 21, 259-263, 2008.) Evaluation experiments were conducted. The experimental procedure is as follows.

The test compound was orally administered to male rats (6 weeks old, Sumatoco Bio Korea) for 30 minutes, and the test compound was intraperitoneally injected with phenol red (200 mg / kg dose) And phenol red dissolved in physiological saline).

② After 30 minutes, anesthesia was performed with diethyl ether, and the abdominal aorta was cut to bleed, and then the entire trachea was excised.

③ Separated organs were washed with 1 ml of physiological saline solution for 30 minutes and centrifuged at 10,000 rpm for 5 minutes at room temperature. 1 N sodium hydroxide (NaOH) was added (0.1 ml of 1N NaOH per 1 ml of supernatant ).

(4) The absorbance at 546 nm was measured to evaluate the drought activity as the concentration of the phenol red discharged.

1-2. Experiment result

Table 1 below is a table showing the sputum-releasing ability according to the administration dose of ambroxol used as a positive control drug and the mixed extract prepared.

division Dosage (mg / kg) Sputum Emission Rate (%) HAE-01 200 17 HAE-02 200 30 HAE-05 200 56 HAE-06 200 87 Ambroxol 250 91

As a result, it was found that the sputum-producing ability of ethanol extract (HAE-05) of golden, quartz, and japonica was higher than that of golden and Gyungyang ethanol extracts (HAE-01) The ethanol extract (HAE-06) of 3% acetic acid in gold, silver and manganese, etc., has a higher sputum emission ability than ethanol extract (HAE-02) containing 3% acetic acid.

Therefore, it was found that the extracts of golden, quartz, and kokkuk extracts were more excellent in the sputum-releasing ability than the iron and the gold and Gyokyung combinations, although they were extracted by the same method and solvent.

In addition, ethanol extracts containing 3% acetic acid showed better sputum emission than ethanol extracts.

In particular, HAE-06 experimental group has higher efficacy than spleen-releasing ability of ambroxol used as positive control in comparison with dose.

FIG. 1 shows the sputum-releasing activity of the mixed extract, which is a graph measuring the concentration of the released phenol.

As a result, it was confirmed that the concentration of amoxicillin phenol in the positive control group was about twice that of the control group.

The HAE-01, 02, 05, and 06 experimental groups showed an increase in phenol concentration compared with the control group, indicating that they had sputum-releasing ability.

(HAE-05 and HAE-06) showed significant sputum-releasing ability compared to the extracts of golden and Gyungyang mixed extracts.

Experimental Example  2. Evaluation of Chinhae activity

2-1. Experimental course

In order to measure the activity of the mixed extract, Tanaka et al. (Motomu Tanaka and Kei Maruyama, J. Pharmacol. Sci., 93, 465-470, 2003. Daoui, Cognon, Naline et. J. Respir. Crit Care, Med., 158, 42-48, 1998.). The experimental procedure is as follows.

After 30 minutes of oral administration of the drug to a male ICR mouse (6 weeks old, Orient Bio), the mice were placed in a plexi-smear chamber (Buxco, USA) and cough induced by spraying a cough inducer (citric acid).

Theobromine (50 mg / kg) was used as a positive control, and mice were exposed to 0.4 M citric acid for 10 minutes, and the cough water generated was measured at intervals of 5 seconds for 10 minutes.

2-2. Experiment result

Table 2 below is a table showing cough inhibition according to the dose of theobromine used as a positive control drug and the mixed extract prepared.

division Dosage (mg / kg) Coughing / 5 seconds Control group - 2.44 ± 0.24 HAE-01 200 2.55 + - 0.33 HAE-02 200 2.67 ± 0.34 HAE-05 200 2.99 + - 0.43 HAE-06 200 2.04 ± 0.33 Theobromine 50 2.31 ± 0.17

As a result, the number of coughs in the HAE-06 group was the lowest at 2.04 ± 0.33, which is lower than that of the group treated with theobromine, the positive control, 2.31 ± 0.17.

FIG. 2 is a graph showing the cough suppressing ability of the mixed extract, which is a measurement of the number of coughs occurring for 5 seconds.

As shown in FIG. 2, the number of coughs decreased in the theobromine-treated group compared to the control group.

In addition, it was confirmed that the coughing frequency of HAE-06 was the lowest, and it was confirmed that the 3% ethanolic extract of acetic acid of gold,

Experimental Example  3. Antioxidant activity evaluation

3-1. Experimental course

The DPPH assay is one of the most common experiments to measure antioxidant efficacy. The principle of DPPH assay is that the deep purple Diphenylpicrylhydrazyl is reduced to Diphenylpicrylhydrazine by the antioxidant and turns into a pale yellow. The stronger the antioxidant power of the antioxidant, the closer to the pale yellow.

Also, when measuring the absorbance, a dark purple color is detected but a light yellow color is not detected. Therefore, the smaller the absorbance value is, the higher the antioxidant power is. The experimental procedure is as follows.

① 8 groups of 0.1 mM DPPH solution (Diphenylpicrylhydrazyl) were prepared.

② Group 1 was set as negative control and no manipulation was performed. Group 2 was set as positive control and treated with ascorbic acid (Vitamin C) 50M.

③ In the experiment group, the extracts were treated with 10 g / mL, 30 g / mL and 100 g / mL, respectively.

④ We observed how the color of the samples changed using a light absorber at a wavelength of 520 nm.

⑤ The measured absorbance was converted in% by the following equation.

3-2. Experiment result

The following Table 3 shows the antioxidative effect of the mixed extracts. The antioxidative effect of the mixed extracts is shown in Table 3, and FIG. 3 is a graph showing the antioxidative effect of the mixed extracts.

division Treatment volume ([mu] g / ml) Antioxidative effect(%)
HAE-01
10 12.1 30 69.2 100 48.8
HAE-02
10 11.2 30 72.1 100 70.3
HAE-05
10 9.3 30 72.8 100 64.2
HAE-06
10 13.2 30 72.3 100 67.5 Vitamin C 50 μM 76.3

As a result, antioxidant activity was observed in all experimental groups and increased in concentration dependent manner. However, it was confirmed that the antioxidative activity of the 100 ㎍ / ㎖ treated group was slightly reduced compared to the 30 ㎍ / ㎖ treated group.

At 30 ㎍ / ㎖ treatment, the antioxidant activity of the mixed extracts showed similar antioxidative activity to ascorbic acid (Vitamin C), which is a positive control.

Claims (5)

Ginseng, and rhubarb, and a mixture of gold,
The mixed extract is mixed with 5 to 10 parts by weight of potash, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of jade,
Extracted with ethanol containing 0.5 to 5% of ethanol or acetic acid as a solvent,
A pharmaceutical composition for chewing gum or dandruff comprising an extract of Huangdong, gold, quartz and jade, as an active ingredient, characterized by having sputum-releasing ability, cough suppressing ability and antioxidant efficacy.
delete delete delete Ginseng, and rhubarb, and a mixture of gold,
The mixed extract is mixed with 5 to 10 parts by weight of potash, 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of ginseng and 0.5 to 1.5 parts by weight of jade,
Extracted with ethanol containing 0.5 to 5% of ethanol or acetic acid as a solvent,
Wherein the composition has a sputum-releasing ability, a cough suppressing ability, and an antioxidant activity as an effective ingredient.

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