KR101722181B1 - Cosmetic composition containing Polydeoxyribonucleotide - Google Patents

Abstract

The present invention relates to a cosmetic composition including polydeoxyribonucleotide (PDRN). More specifically, the present invention relates to a cosmetic composition including PRDRN, wherein the cosmetic composition ensures elasticity reinforcement, water gloss moisturization, and whitening from the base layer of skin by using PDRN, glutathione, and hyaluronic acid as main ingredients. The cosmetic composition comprises: 0.1-5 wt% of water and PDRN; 1-7 wt% of glutathione; 5-20wt% of hyaluronic acid; 0.5-3 wt% of butylene glycol; 0.1-1 wt% of acetyl hexapeptide-6; and 0.1-0.5 wt% of palmitoyl pentapeptide-4. The cosmetic composition of the present invention is produced as a cosmetic liquid or a skin coating ampoule, and is coated on a face or skin. Then a mask pack is attached to the face or the skin, and an absorption rate is improved. Thus, the cosmetic composition can be conveniently used, and ensures elasticity reinforcement, water gloss moisturization, and whitening from the base layer of skin.

Description

Cosmetic composition containing polydeoxyribonucleotide (PDRN)

The present invention relates to a cosmetic composition containing PDRN (polydeoxyribonucleotide), and more particularly, to a cosmetic composition comprising PDRN, glitachione and hyaluronic acid as main components and a PDRN containing PDRN having elasticity strengthening, water light- ≪ / RTI >

Polydodeoxyribonucleotides (PDRN) are known to regenerate tissue. PDRN is produced from DNA extracted from salmon semen.

PDRN is used for treatment of wound due to skin graft and tissue repair. However, it is used according to medical judgment even for some tissue regeneration or inflammation treatment without proper treatment, and stimulates A2 receptor as a skin regeneration signal transduction to secrete various growth factors It is known as a component that functions to promote the formation of capillaries by VEGF (vascular endothelial cell growth factor), improve blood circulation, anti-inflammatory action and capillary leak.

Recently, PDRN has been used for wound healing and normal organization as well as for acne scars, accident scars, stretch marks, skin atrophy, It has been developed as a skin-improving cosmetic product with various functions such as acne skin, stain and dirt, but it is not enough to develop effective products yet.

When the glutathione and hyaluronic acid were mixed with PDRN, the inventors of the present inventors confirmed that the PDRN can be solved at a time from the basal layer of the skin to the elasticity strengthening, the water-light enhancement and the whitening, And a PDRN that can be used as a cosmetic.

It is an object of the present invention to provide a cosmetic comprising PDRN, PDRN, glutathione and hyaluronic acid as a main component, which can be used as a cosmetic lotion having skin elasticity strengthening, water light moisturizing and whitening function or skin emulsifying ampoule.

In order to attain the above object, the cosmetic composition containing PDRN of the present invention is characterized by containing 0.1 to 5% by weight of PDRN in the whole composition.

Also, the cosmetic composition comprising PDRN is characterized by comprising 0.1 to 5% by weight of PDRN, 1 to 7% by weight of glutathione and 5 to 20% by weight of hyaluronic acid in the total weight ratio.

The cosmetic composition comprising PDRN may further comprise water and 0.1 to 5 wt% of PDRN, 1 to 7 wt% of glutathione, 5 to 20 wt% of hyaluronic acid, 0.5 to 3 wt% of butyleneglycol, And 0.1 to 0.5% by weight of palmitoyl pentapeptide-4.

The present invention provides a cosmetic composition comprising PDRN having elasticity strengthening, water light moisturizing and whitening function from the skin base layer.

The present invention provides a method for producing a cosmetic composition comprising PDRN having a function of enhancing elasticity, water retention, and whitening function from the skin base layer.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a comparative photograph of skin elasticity and whitening effect before and after using the cosmetic composition of the present invention.

The cosmetic composition comprising the PDRN of the present invention is characterized by comprising 0.1 to 5% by weight of PDRN in the whole composition.

The cosmetic composition comprising the PDRN of the present invention is characterized by comprising 0.1 to 5% by weight of PDRN, 1 to 7% by weight of glutathione and 5 to 20% by weight of hyaluronic acid in the total weight ratio.

In addition, the cosmetic composition containing PDRN of the present invention may contain water and 0.1 to 5 wt% of PDRN, 1 to 7 wt% of glutathione, 5 to 20 wt% of hyaluronic acid, 0.5 to 3 wt% of butyleneglycol, 0.1 to 1 wt% and palmitoyl pentapeptide-4 0.1 to 0.5 wt%.

PDRN (polydeoxyribonucleotide) plays a role in regenerating damaged skin cells and anti-inflammatory action, and to strengthen skin elasticity from the base layer.

The content of PDRN is preferably 0.1 to 5% by weight based on the total weight of the composition. If the amount is less than the above range, the PDRN is undesirable because it has a whitening function and skin regenerating function due to antioxidation of PDRN. Which is undesirable.

Glutathione acts to reduce melanin pigment, that is, oxidized pigment in the skin, and excretes itself out of the skin. It maintains the intracellular physiological concentration of 5 mM and at the same time applies antioxidant technology to glutathione, To balance the homeostasis and skin whitening as well as PDRN helps to regenerate the cells together. Glutathione is an antioxidant that plays an important role in detoxification and maintains the thiol state in the body. Reduced glutathione (GSH) is known to have a whitening effect

The content of glutathione is preferably 1 to 7% by weight based on the total weight of the composition. If the amount is less than the above range, the whitening function due to antioxidation and the skin regenerating function are undesirable, and if it exceeds the above range, I do not.

Hyaluronic acid (sodium hyaluronic acid) is a complex polysaccharide consisting of amino sugar and uronic acid. It is a major mucopolysaccharide with chondroitin sulfate and is widely distributed in all tissues of the animal, especially in the insect tissues, and is extracted from umbilical cord or chickens. , Prevents penetration of bacteria into the skin or penetration of toxic substances, and has an excellent effect in moisturizing.

The content of hyaluronic acid is preferably 5 to 20% by weight based on the total weight of the composition. If the content is less than the above range, the skin absorption rate is low and the moisturizing function is poor, which is undesirable. .

Butylene Glycol is mainly used as a cosmetic solvent and has little irritation and toxicity to the skin. It plays a role of moisturizing agent because it has the function of dissolving the components of cosmetics and attracting moisture.

Acetyl hexapeptide (Acetyl Hexapeptide-8) strengthens the skin and improves skin elasticity. It increases the amount of collagen and elastin, and is effective in improving wrinkles.

Palmitoyl Pentapeptide-4 stimulates collagen and elastin production in the dermis, which can easily penetrate the skin with fewer molecules. It does not itch or irritate the skin, it restores damaged skin against light damage, and is effective in preventing wrinkles, and has an excellent effect in preventing skin aging.

The water used in the cosmetic composition containing the PDRN of the present invention may be any one of a plant extract, a fruit extract, a plant fermentation broth, or a fruit fermentation broth including purified water, nodular sap, tea extract, green tea extract, bamboo extract, One or more mixed liquids may be included. In addition, the cosmetic composition containing the PDRN of the present invention can be manufactured using cosmetic lotion or skin emulsifying ampoule, etc., and can be conveniently applied to the face or skin by applying a mask pack to increase the absorption rate.

Hereinafter, a method for preparing a cosmetic composition comprising the PDRN of the present invention will be described in more detail by way of examples. The present invention is described in more detail with reference to the following examples, but the scope of the present invention is not limited to these examples.

≪ Example 1 >

10 g of PDRN, 20 g of glutathione, 100 g of hyaluronic acid, 10 g of butylene glycol, 2 g of acetylhexapeptide-6, 2 g of palmitoylpentapeptide-4 and 856 ml of purified water were mixed and passed through a liquid dispersion emulsification apparatus And then sterilized and vacuum packed to prepare 1 liter of lotion.

≪ Example 2 >

10 g of PDRN, 100 g of hyaluronic acid, 10 g of butylene glycol, 2 g of acetylhexapeptide-6, 2 g of palmitoylpentapeptide-4 and 876 ml of purified water were mixed and passed through a liquid dispersing and emulsifying device, sterilized and vacuum- .

≪ Example 3 >

10 g of PDRN, 20 g of glutathione, 10 g of butylene glycol, 2 g of acetylhexapeptide-6, 2 g of palmitoylpentapeptide-4 and 956 ml of purified water were mixed, passed through a liquid dispersion emulsifier, sterilized and vacuum packed to prepare 1 liter of lotion Respectively.

<Comparative Example>

10 g of butylene glycol, 2 g of acetyl hexapeptide-6, 2 g of palmitoyl pentapeptide-4 and 986 ml of purified water were mixed, passed through a liquid dispersion emulsifier, sterilized and vacuum packed to prepare 1 liter of lotion.

&Lt; Test Example 1 >

Skin irritation test

The skin lotions prepared according to Examples 1 to 3 and Comparative Examples of the present invention were applied to the upper forearm of 10 subjects of 20 to 50 years old women using Finn chambers for 6 hours with occlusive patches ) And 30 minutes after removal of the patch, the degree of irritation was determined according to the irritation criteria. 0.1% lactic acid was used as a control stimulus source. The results are shown in Table 1, and the stimulus was calculated according to the following formula.

As a result of the test, the skin lotions produced by Examples 1 to 3 and Comparative Examples of the present invention showed little skin irritation.

Equation: Stimulus degree = ((+ number × 1) + (++ number × 2) + (+++ number × 3)) /

Skin irritation test results
division
Number of subjects Test results
Irritation degree _ + ++ +++ Lactic acid 10 5 2 2 One 0.9 Example 1 10 10 0 0 0 0 Example 2 10 10 0 0 0 0 Example 3 10 10 0 0 0 0 Comparative Example 10 9 One 0 0 0.1

*Criteria

-: No erythema or unusual phenomenon

+: Slightly reddish from around

++: significantly more red than ambient

+++: More reddened and swollen than around

&Lt; Test Example 2 &

Moisture Retention Test

Water retention was tested on the lotion prepared according to Examples 1 to 3 and Comparative Example of the present invention. 10 subjects of 20-50 age-old females were applied with 1 ml / 10 cm 2 of the lotion prepared in Examples 1 to 3 and Comparative Example in a constant temperature and humidity room maintained at 22 ° C and 50% relative humidity, After 3 hours, skin moisture content was measured using a skin moisture analyzer (my-808s, manufactured by Scala Co., Ltd., Japan). The results are shown in Table 2. The water holding power is shown in Table 2, which is the average value of the water holding value of the subjects.

As a result, it was shown that the water retention capacity of the lotion prepared in Examples 1 to 3 and Comparative Example of the present invention was increased, and the lotion of Example 1 had the highest moisture retention.

Moisture Retention Test Results Water content (%) Before application After application City 37.6 45.3 City 37.6 42.5 City 37.6 43.2 37.6 39.1 37.6 37.7

&Lt; Test Example 3 >

Skin whitening effect

Skin whitening effect was tested on the cosmetic lotions prepared by Examples 1 to 3 and Comparative Examples of the present invention. For 10 subjects in their 20s and 50s, an opaque tape with five holes each having a diameter of 1.5 cm was attached to the upper side of the subject, and the UV rays (UVB) of about 1.5 to 2 times of the minimum erythema amount of each subject were examined, After inducing the blackening, 2.5 ml of the lotion prepared according to Examples 1 to 3 and Comparative Example of the present invention was applied every morning and evening, and after 4 weeks, the darkness and darkness of the skin were measured using a colorimeter. Skin contrast is expressed as an 'L' value and represents a value between 0 (black) and 100 (pure white) (Koreans typically have values of 50 to 70). The shade of skin is the final measured value after application - the initial measured value before application is shown in Table 3.

As a result, it was confirmed that the skin complexity in Examples 1 to 3 of the present invention was significantly higher than that of the control and the control (purified water).

Skin whitening effect test result
division Skin color (L) 0 weeks before application One week after application Two weeks after application Three weeks after application 4 weeks after application Example 1 56.4 57.2 58.0 59.5 61.7 Example 2 56.4 57.2 57.6 58.1 58.7 Example 3 56.4 57.0 57.4 57.8 58.4 Comparative Example 56.4 56.8 57.0 57.3 57.6 Control
(Purified water) 56.4 56.5 56.7 56.9 57.1

<Test Example 4>

Exclusive clinical tests such as skin elasticity and whitening

Using two skin lotions prepared according to Example 1 of the present invention, the two subjects were subjected to an overall skin improvement test such as skin elasticity and whitening. In the test method, 2.5 ml of the lotion prepared according to Example 1 of the present invention was first applied to the face and neck region after cleansing the subject, and MTS (micro needle treatment) procedure was performed. Then, After applying 2.5 ml of the skin lotion manufactured by the manufacturer, it is peeled off after putting the water pack for 30 to 40 minutes. The following day, 2.5 ml of the lotion of Example 1 of the present invention was applied to the face and neck twice in the morning and evening, followed by tapping twice. The skin condition was checked 5 times in total, Respectively.

As a result, it was confirmed that the skin lotion prepared in Example 1 of the present invention improved skin elasticity and moisturizing degree on the face and neck as a whole, and skin whitening effect could be confirmed.

Claims (3)

delete delete A cosmetic composition comprising PDRN (polydeoxyribonucleotide) at a total weight ratio comprises water, 0.1 to 5 wt% of PDRN, 1 to 7 wt% of glutathione, 5 to 20 wt% of hyaluronic acid, 0.5 to 3 wt% of butyleneglycol, -6 0.1 to 1 wt%, palmitoyl pentapeptide-4 0.1 to 0.5 wt%, based on the total weight of the cosmetic composition.

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