KR101686483B1 - Process for the manufacture of pearl proteins solubilization and grateloupia elliptica and hair cosmetics obtained by the process - Google Patents

Abstract

The present invention relates to a method for obtaining a pearl protein solubilized material and Grateloupia elliptica extract, and a hair cosmetic product comprising the pearl protein solubilized material and Grateloupia elliptica extract obtained through the method as active ingredients. According to the present invention, a hair cosmetic product having a pearl protein solubilized material and Grateloupia elliptica extract as active ingredients can prevent hair damage, improve elasticity and shine of hair, and help improvement of damaged hair. The method comprises: a preparation step of preparing pearl powder; an alkali solubilization step; a stabilization step; a solubilized material concentration step using clathrate hydrolysis; a filtration and separation step; an obtaining step of obtaining a pearl protein solubilized material; drying and grinding Grateloupia elliptica, which is one of red algae, and manufacturing powder; a hydrolysis step; a neutralizing step; and a Grateloupia elliptica extract obtaining step.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for obtaining a pearl protein-soluble substance and a gum extract, a cosmetic product for pearl proteins solubilization and grateloupia elliptica and hair cosmetics obtained by using the pearl protein- }

The present invention relates to a method for obtaining a pearl protein-soluble substance and a gum extract, and a cosmetic for hair using as an active ingredient a pearl protein-soluble substance and a gum extract obtained by the method. More specifically, the present invention provides a hair cosmetic product comprising a pearl protein-soluble substance and a gum extract as an active ingredient, thereby preventing damage to the hair from the outside and improving the volume, elasticity, gloss and smoothness of the hair to improve damaged hair And a cosmetic for hair using as an active ingredient the pearl protein-soluble substance and the gum extract obtained by the method.

Hair has the characteristics of being easily damaged from external environment. Recently, modern hair is severely damaged due to the use of frequent salt preparation and use of permers, excessive dryers, and ultraviolet rays, and hair is dry and frizzy, and gloss, softness and smoothness are deteriorated.

Accordingly, in the hair cosmetics such as hair serum and water essence, hair products capable of preventing damage to hair, enhancing glossiness, smoothness, ease of combing, volume feeling, or ability to evacuate are continuously on the market, and expectation Is increasing.

Conventional hair products can improve the above effects by using a conditioning agent such as a combination of silicone, hydrocarbon water, and fatty ester, but these components have drawbacks that cause hair to become damp and sticky.

Also, in order to promote the conditioning of the hair, a hair product which can improve the moistness, gloss, elasticity and flexibility of the hair by using the hydrolyzed conchiolin extract or the soy protein is developed, There is a limit to lowering the hair gently and smoothly.

Prior Art Document: KR Patent Registration No. 1337892 (registered on December 2, 2013)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a hair- It is an object of the present invention to provide a method for obtaining a cargo and gum extract, and a cosmetic for hair using as an active ingredient a pearl protein-soluble substance and a gum extract obtained by the method.

A method for obtaining a pearl protein-soluble substance and a gum extract according to the present invention, which is devised to achieve the above object, and a hair cosmetic comprising an active ingredient of a pearl protein-soluble substance and a gum extract obtained by the method, Preparing to prepare; (b) mixing the pearl powder prepared in step (a) with an alkali agent having a mass ratio of 20% or more of the protein content in the pearl powder and heating and stirring the mixture at a temperature of 100 or higher for 2 hours or more to completely digest peptides and amino acid constituent units An alkali solubilizing step of performing an alkali solubilization step; (c) adding an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid to the solubilized reaction product in the step (b) to neutralize the solution to a pH range of 8 or more to a pH of 9 or less to prevent precipitation of oligomeric or higher polymeric peptides ; (d) The solubilized material and mineral water obtained in step (c) are introduced into a high-pressure reactor having an atmosphere of carbon dioxide, and are concentrated under conditions of 30 atm and 0 to 4 so as to obtain a lattice structure The gas containing carbon dioxide is trapped in the high-pressure reactor, so that the hydrate of the gas composed of carbon dioxide and volatile basic nitrogen introduced into the high-pressure reactor is removed by the hydrate of the solubilized material, Concentration step of the soluble fraction; (e) filtration and separation step of separating the hydrate having formed the cluster hydrate and the remaining soluble matter by transferring the base oil-removed solvate to the separator in the step (d); (f) obtaining a chopped protein solvate isolated in step (e); (g) Drying and pulverizing gambling, a kind of red algae, to prepare a powder; (h) hydrolyzing 1 to 3 hours at 80 to 100 ° C under 1 atm of an acid catalyst containing 10 to 30% citric acid based on the weight of the raw material; (i) removing unreacted residues remaining in the hydrolyzed product in step (h); (j) an inorganic salt catalyst containing sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, and hydrolyzed product under acid catalyst in step (h) in an amount of 80 to 100% of the added amount of acid catalyst added in step (h) And neutralizing the solution to a pH within a range of 5 to 9; (k) concentrating the hydrolyzed neutralized neutralized in step (j) in the range of 5 to 30 Brix; And (l) after step (k), obtaining a gambling extract.

The method may further include (m) mixing the resultant product of step (f), the pearl protein-soluble substance, with the gambling extract as a result of step (1).

In addition, the cosmetic for hair includes the pearl protein-soluble substance obtained by the above-mentioned method and the gambling extract as an active ingredient, and the hair cosmetic may be a shampoo, a tonic, or a treatment.

According to the present invention, there is provided a method for obtaining a pearl protein-soluble substance and a gum extract, and a hair cosmetic containing the pearl protein-soluble substance and the gum extract obtained by the method, thereby preventing hair damage from the outside, , Elasticity, luster and smoothness of the damaged hair can be improved.

1 is a flow chart of a method of obtaining a pearl protein-soluble substance and a gum extract according to a preferred embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. In addition, the preferred embodiments of the present invention will be described below, but it is needless to say that the technical idea of the present invention is not limited thereto and can be variously modified by those skilled in the art.

1 is a flow chart of a method of obtaining a pearl protein-soluble substance and a gum extract according to a preferred embodiment of the present invention.

Referring to FIG. 1, a method of obtaining a pearl protein-soluble substance and an extract of gum according to a preferred embodiment of the present invention comprises preparing a pearl powder (S10), an alkali solubilizing step (S20), a stabilizing step (S30) (Step S40), filtration and separation step (S50), step (S60) of obtaining a pearl protein soluble product (S60), preparing a gummy powder (S70), hydrolyzing step (S80), filtering step A neutralization step (S100), a gambling extract obtaining step (S110), and a mixing step (S120).

Hereinafter, the preparation of the pearl powder (S10) will be described.

The pearl powder preparing step (S10) is a step of preparing pearl powder.

The pearl contains an ingredient such as amino acids and other organic substances, and has an excellent effect of whitening whitish pigment such as stain. In addition, eating and applying pearls will moisturize and moisturize the skin, prevent aging of the skin, and contain ingredients such as age-old spots, freckles, rashes, acne and skin boils.

These pearls are crushed and prepared into powder.

The alkaline solubilization step (S20) comprises mixing the pearl powder prepared in step (S10) of preparing the pearl powder with an alkali agent having a mass ratio of 20% or more of the protein content contained in the pearl powder, and heating and stirring the mixture at a temperature of 100 or higher for 2 hours or longer to form a peptide To complete the solubilization.

More specifically, the alkali agent to be mixed in the alkali solubilization step (S20) is characterized by using a weak alkali or strong alkali comprising calcium carbonate, sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide.

In addition, if less than 20% of the alkaline agent of the protein content contained in the pearl powder is used in the alkali solubilization step (S20), incomplete solubilization leads to complete solubilization.

Here, complete solubilization means that the protein is hydrolyzed to the peptide and amino acid building block, and the incomplete solubilization is not hydrolyzed to the peptide and amino acid building block.

In the alkali solubilization step (S20), it is preferable to stir with at least 80 degrees Celsius, mainly 100 degrees Celsius. The minimum reaction temperature required for complete hydrolysis of the protein is 80 degrees Celsius, , It is difficult to completely solubilize the polymer peptide because oligomeric or higher polymeric peptides are mainly formed. Therefore, it is preferable to conduct the reaction at a temperature of 100 DEG C or higher.

In addition, it is preferable to stir the solution in the alkali solubilization step (S20) at a temperature of 100 degrees Celsius or more for 2 hours or more, since this is the minimum reaction time required for complete hydrolysis of the protein.

In the stabilization step (S30), an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid is added to the reacted solubilized product in the alkali solubilization step (S20) to neutralize the solution to a pH range of 8 or more to a pH of 9 or less to precipitate the oligomeric or higher oligomeric peptide .

In this case, in the stabilization step (S30), the acidic aqueous solution is added to neutralize the solution to a pH of from 8 to less than pH 9, whereby the polymeric peptides of oligomeric or higher oligomeric or higher than the unreacted ones in the neutral or acidic range below pH 7 may be precipitated in the aqueous solution, It can not be used as a cosmetic material (cosmetics) when the pH exceeds 9.

In the concentration step (S40) of the solubilization using the crystallization hydrate, the soluble material and the mineral water, which have been subjected to the stabilization step (S30), are introduced into a high-pressure reactor having an atmosphere of carbon dioxide and concentrated under conditions of 30 atm and 4 , The gas containing carbon dioxide is trapped in the lattice structure of the mixture of the soluble and mineral water, so that the hydration degree of the gas constituted by the carbon dioxide and volatile basic nitrogen introduced into the high-pressure reactor is removed as much as the moisture of the soluble material is removed The base nitrogen in which the ammonia odor remains is removed.

The hydrate is trapped in the space where the water molecules are formed by the hydrogen bonding, so that the gas containing carbon dioxide is trapped.

In addition, the concentration by the use of the clathrate hydration is controlled by controlling the composition of a specific gas and liquid, so that water as a subject molecule surrounds a gas, which is an object molecule, to form a crystal of a hydrate. Therefore, Since only pure water is consumed to combine with gas molecules to form a hydrate, it is possible to separate it from the solution including the gaseous substance present in the raw material solution, and the amount of water consumed to form the hydrate The concentration of the solution can be controlled by adjusting the amount of water consumed so that the concentration of the solution increases.

In the present invention, since hydrate is formed by the combination of base nitrogen remaining in ammonia odor generated by hydrolysis of pearl protein and mineral water which is pure water by using pure water hydration, pure water is consumed, It is possible to separate and concentrate the concentrated high-concentration pearl protein-soluble carbohydrate by easily removing ammonia from the base nitrogen due to the hydrolysis of the pearl protein, which is a conventional problem by improving the productivity and economical efficiency Of course.

Particularly, the reaction condition for performing hydration so that the gas containing carbon dioxide is trapped in the mixture of the soluble matter and the mineral water put into the high-pressure reactor in the concentration step (S40) of the solubilization using the clathrate hydration is 30 atm 4 or less is preferable.

This is because, as the phase equilibrium condition for forming the hydrate, hydrate formation does not occur when the pressure is 30 atmospheric pressure, and when the pressure is 30 atmospheric pressure, especially when the pressure is less than 0, all of the reactant forms hydrate, thereby eliminating volatile basic nitrogen and concentrating the solute It is difficult to separate the interface.

In addition, it is preferable that the reaction time of the carbon dioxide hydrate in the concentration step (S40) of the solubilized material using the clathrate hydration is generally 30 minutes or more and 1 hour or less.

This is the induction time, which is determined by the phase equilibrium arrival time of the gas hydrate. If the reaction time is less than 30 minutes and the reaction time is more than 1 hour, it is not suitable for the phase equilibrium reaching time. It is difficult to remove the nitrogen and to separate the interface for concentration of the solute.

In the filtration and separation step (S50), the solubilized material in which the base nitrogen has been removed in the concentration step (S40) of the solubilized material using the clathrate hydrate is transferred to a separator through a filter including a microfilter in a high pressure reactor, Is separated from the remaining volatiles from which base nitrogen has been removed.

The step of obtaining the pearl protein-soluble product (S60) is a step of obtaining a solubilized product in which the volatile basic nitrogen is removed while passing through the filter and the separator in the filtration and separation step (S50).

The concentrated pearl protein soluble substance thus obtained is used as a flour material (cosmetic material).

The gambling powder preparing step (S70) is a step of preparing gambling which is a kind of red algae.

Gambling is a seaweed with red algae genus aleit, 15 ~ 40cm in height, leaf-shaped and divided into several branches. Red, purple, yellow, green flesh, it turns purple when dried. It is used as a grass-making material or a glue-making material together with grass pulp. It is distributed in Korea, Japan and so on.

The gambling type of red contains L-methionine, which is a kind of α-amino acid containing sulfur. L-methionine is a sulfur amino acid (amino acid containing a sulfur (S) in a molecule such as methionine, cysteine, and homocysteine) constituting a protein, and functions as a methyl donor in a living body although its content in the protein is small. In addition, L-methionine is an essential amino acid absolutely necessary for animal growth. It is a precursor of cystine, a hair-forming substance, and is a substance capable of supporting hair growth.

As described above, gums having an effect on the growth of hair are dried and pulverized to prepare powders.

The hydrolysis step S80 may be performed at 1 atm, 80 to 100 deg. C for 1 to 3 hours using an acid catalyst of 10 to 30% based on the total weight of the gambling powder and gambling prepared in the gambling powder preparation step (S70) . ≪ / RTI >

If the content of the acid catalyst in the hydrolysis step (S80) is less than 10%, the hydration reaction of the alginic acid contained in the gambling may be partially accompanied thereby causing clogging in the subsequent filtration step (S90) , The effect on the curing reaction of alginic acid contained in the gambling is not significantly different from the concentration of 30% or less.

Alginic acid is an acidic polysaccharide having excellent water ability (ability to water). Since it has a high hydration degree to water, a large amount of water and a hydration reaction are performed, and then swelled to cause mucilaginous gelation. Therefore, in the next filtration step (S90) It is preferable that the pores of the filter can be blocked and the separation step is performed in the hydrolysis step (S80).

In addition, when the reaction temperature in the hydrolysis step (S80) is less than 80 degrees Celsius, the diffusion rate of water into the gambling is slow and the hydrolysis reaction rate is reduced. When it exceeds 100 degrees Celsius, the dielectric constant of water is low, so that the ion exchange capacity of the acid catalyst decreases, and the rate of hydrolysis reaction decreases.

In addition, citric acid or the like can be used as the acid catalyst used in the hydrolysis step (S80), and the kind of the acid catalyst is not limited to a specific one.

When the reaction time is less than 1 hour in the hydrolysis step (S80), hydrolysis yield is low due to low hydrolysis yield. If the reaction time exceeds 3 hours, peroxidation reaction and carbonization reaction due to long- There is a possibility of generation of peroxide or carbide.

The filtration step (S90) is a step of removing the unreacted residue remaining in the hydrolyzate. Here, unreacted residues are mainly saccharides contained in a raw material that does not exhibit hydrolytic reactivity, and other substances such as fibrin, proteins, lipids, and the like that have not undergone the hydrolysis reaction may be included.

In the neutralization step (S100), the inorganic salt catalyst is added to the organic acid catalyst hydrolysis reaction product in the hydrolysis step (S80) within the range of 80 to 100% of the added amount of the acid catalyst, and neutralized to a pH of 5 or more and a pH of 9 or less to be.

The inorganic salt catalyst used in the neutralization step (S100) may be any one of sodium carbonate, sodium bicarbonate, sodium hydroxide, and potassium hydroxide. The type of the inorganic salt catalyst is not limited thereto.

 If the addition amount of the inorganic salt catalyst is less than 80%, the neutralization value has a hydrogen ion concentration of less than pH 5, so that it may cause transdermal stimulation when the cosmetic agent is used. If the addition amount exceeds 100%, the neutralization value exceeds the pH 9 It may cause transdermal stimulation when using cosmetics.

The concentration step (S110) is a step of concentrating the hydrolyzed neutralized product to adjust the concentration within the range of 5 brix to 30 Brix. More preferably, in the concentration step (S110), the hydrolyzed neutralized product is concentrated to 20 Brix using a vacuum and vacuum concentrator to obtain 100 ml of a concentrate.

When the concentration of the hydrolyzed neutralized product is 5 Brix in the concentration step (S110), it is the normal concentration level of the initial hydrolyzed neutralized product. When the concentration of the hydrolyzed neutralized product exceeds 30 Brix, the solubility of the saccharide in the concentrated product may be recrystallized by supersaturation. , The amount of the solubilizing agent and ethanol may increase in the formulation step of the cosmetic preparation to be performed thereafter, which may result in a range limitation of the formulation prescription.

The step of obtaining gambling extract (S110) is a step of obtaining a concentrated gambling extract using a vacuum and pressure-reducing concentrator in a concentration step (S110).

[Example 1] Method for obtaining gambling extract

100 g of gambling powder was prepared by dry grinding the gambles, 30 g of citric acid was dissolved in 1 L of purified water, and the mixture was hydrolyzed at 100 DEG C for 1 hour to separate the hydrolyzate and residue (filtrate) from the filtrate to obtain 400 mL of hydrolyzate , And the hydrolyzate concentration at this time is about 5 Brix. Next, 24 g of sodium hydrogencarbonate is neutralized by mixing with the obtained hydrolyzate to obtain 400 ml of a neutralized product. At this time, the hydrogen ion concentration of the hydrolyzed neutralized product is pH 5. Next, the hydrolyzed neutralized product is concentrated to 20 Brix using a vacuum decompression concentrator to obtain 100 ml of a concentrate.

The mixing step (S120) mixes the resultant product of step S60, the pearl protein-soluble substance, with the gum extract, which is the result of step S110.

This mixed pearl protein soluble carbohydrate and gambling extract is used as a flour material (cosmetic).

The cosmetic for the hair comprising the pearl protein extract and the gum extract obtained by the method of obtaining the pearl protein extract according to the preferred embodiment of the present invention as an active ingredient is characterized in that it is a shampoo, a tonic, or a treatment, In the description, a composition of shampoo, toner, and treatment will be specifically described.

Ⅰ. Shampoo composition comprising pearl protein extract and gambling extract as active ingredient

The shampoo composition of the hair cosmetic composition may be at least one selected from the group consisting of polyquaternium-10, sodium laureth (3) sulfite, decyl glucoside, cocamaradopropyl betaine, DL-panthenol, polyquaternium-7, pH adjusting agent, paraoxybenzoic ester, Made DEA, aroma oil, combination fragrance, pearl protein soluble substance, gambling hydrolyzate.

More specifically, the present invention relates to a composition comprising 0.3 wt% of polyquaternium-10, 34 wt% of sodium laureth sulfite, 5 wt% of decyl glucoside, 5 wt% of cocamaradopropylbetaine, 0.2 wt% of DL- 0.4% by weight of sodium hydroxide-7, 0.15% of pH adjusting agent, 0.2% by weight of paraxybenzoic acid ester, 2.5% by weight of cocamide DEA, 0.5% by weight of aroma oil, 2% by weight of combined fragrance, 35.75% By weight.

Ⅱ. A tonic composition comprising pearl protein extract and gambling extract as active ingredients

The tonic composition in hair cosmetics consists of l-menthol, salicylic acid, tocopherol acetate, HCO60, DL-Panthenol, pH adjuster, ethanol, aroma oil, combination fragrance, pearl protein solubilizer and gum hydrolyzate.

More specifically, the present invention relates to a method for producing a liposome comprising 0.1 wt% of 1-menthol, 0.2 wt% of salicylic acid, 0.03 wt% of tocopherol acetate, 0.1 wt% of HCO60, 0.2 wt% of DL-Panthenol, 0.15 wt% 0.03% by weight of a combined fragrance, 58.69% by weight of a pearl protein soluble substance, and 5% by weight of a gambling hydrolyzate.

Ⅲ. Treatment composition comprising pearl protein extract and gambling extract as active ingredient

Among the hair cosmetic compositions, the treatment composition may be selected from the group consisting of glycerin, hydrolyzed keratin, arginine / lysine polypeptide, hyaluronic acid, dipropylene glycol, pH adjuster, phenoxyethanol, aroma oil, HCO60, dimethicone, Soluble carbohydrate, and gum hydrolyzate.

More specifically, it comprises 5% by weight of glycerin, 1% by weight of hydrolized keratin, 1% by weight of arginine / lysine polypeptide, 2% by weight of hyaluronic acid, 2% by weight of dipropylene glycol, , 0.2% by weight of phenoxyethanol, 0.5% by weight of aroma oil, 0.1% by weight of HCO60, 5% by weight of dimethicone, 79.05% by weight of pearl protein solubles and 5% by weight of gum hydrolyzate.

Among the compositions contained in the shampoo, tonic, and treatment containing the above-described pearl protein extract and gambling extract as an active ingredient, the combination flavoring is a substance to be administered to enhance the sustaining power of the fragrance, and natural perfume and synthetic flavoring may be blended, Can be obtained by mixing fragrance. Here, the kind of the combined perfume is not limited to a specific one.

It will be apparent to those skilled in the art that various modifications, substitutions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are intended to illustrate and not to limit the technical spirit of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments and the accompanying drawings . The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

S10 - Pearl Powder Preparation Step
S20 - Alkali Solubilization Step
S30 - Stabilization step
S40 - Concentration of Soluble Compounds by Clathrate Hydration
S50 - Filtration and Separation Step
S60 - Step of obtaining pearl protein soluble product
S70 - Gamma powder preparation step
S80 - hydrolysis step
S90 - Filtration stage
S100 - neutralization phase
S110 - Gamut Extract Step
S120 - mixing step

Claims (3)

(a) a preparation step of preparing a pearl powder;
(b) Completely solubilized by mixing the pearl powder prepared in the preparation step with an alkali agent having a mass ratio of the protein content in the pearl powder of 20% or more and heating and stirring at a temperature of 100 ° C or more for 2 hours or more to hydrolyze the peptide or amino acid constituent unit An alkali solubilization step in which base nitrogen is produced during the hydrolysis;
(c) adding an acidic aqueous solution containing hydrochloric acid, sulfuric acid, acetic acid, and citric acid to the solubilized product that has been reacted in the alkali solubilization step to neutralize the solution to a pH of from 8 to 9, thereby preventing precipitation of oligomeric or higher polymeric peptides;
(d) In order to remove the base nitrogen generated from the solubilized product after the reaction is completed in the stabilization step, the soluble material and water are put into a high-pressure reactor having an environment of carbon dioxide and are concentrated under conditions of 30 atm and 0 ° C to 4 ° C The hydrate in which the soluble cargo and the water form a lattice structure and the gas containing carbon dioxide in the lattice structure is collected is carried out and the water forms a hydrate by surrounding the gas containing the carbon dioxide and the base nitrogen so that the base nitrogen Concentrating the solubles using the crystallite hydration to separate the solids;
(e) filtration and separation step of separating the hydrate forming the cluster hydrate and the remaining solubilized material from which the base nitrogen is removed by transferring the concentrated solubilized product to the separator in the concentration step of the solubilized product using the clathrate hydration;
(f) obtaining a pearl-soluble product separated in the step of filtration and separation;
(g) drying powdered gum (grateloupia elliptica), which is a kind of red algae, to prepare a powder;
(h) performing hydrolysis for 1 to 3 hours at a pressure of 1 atm and 80 ° C or more and 100 ° C or less under an acid catalyst containing 10-30% citric acid based on the gum powder;
(i) removing unreacted residues remaining in the hydrolyzed product in step (h);
(j) an inorganic salt catalyst comprising sodium hydrogencarbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide and the resultant hydrolyzed product under acid catalyst in step (h) is added in an amount of 80 to 100 wt% %, And neutralizing to a pH within a range of 5 to 9;
(k) concentrating the hydrolyzed neutralized neutralized in step (j) in the range of 5 to 30 Brix; And
(l) after step (k), obtaining gambling extract
≪ / RTI > and a gum extract. The method according to claim 1,
(m) mixing the resulting pearl protein-soluble product with step (f) and gambling extract as a result of step (l)
≪ / RTI > further comprising the step of: delete

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