KR101670355B1 - Measurement chamber for behavior and respiro - physiological activity of microorganism - Google Patents

Abstract

A container for measuring microbial activity and respiratory physiological activity is disclosed. The microbial activity and respiratory physiological activity measuring container of the present invention is for measuring and observing microbial behavior and respiratory physiological activity. The container is provided with an upwardly opened accommodation space, and an inlet for flowing circulation water into the accommodation space And a discharge port through which the circulating water of the receiving space is discharged, wherein the bottom of the receiving space is coupled with the bottom member of the transparent material in a hermetic manner; An upper cover of a transparent material which is hermetically coupled to an upper portion of the biological storage member such that the accommodation space is blocked from the atmosphere; First and second valve devices respectively installed at the inlet and the outlet to control the circulating water flowing into the receiving space of the biological storage member and connected to the circulation loop of the respiratory physiological activity measuring device; And a lighting device for shielding scattering of external light flowing into the receiving space and irradiating light to the bottom member for identification of the test organisms. According to the present invention, since the bottom member and the top cover of the biological storage member are made of a transparent material and the illuminating device provided with the white light diffusing plate is provided on the bottom surface of the bottom member, And the receiving space is sealed by the bottom member and the top cover, thereby blocking oxygen from the atmosphere from entering the receiving space, allowing the microorganisms to easily enter and exit, and allowing water to circulate smoothly for a long period of time It is possible to provide the effect of simultaneously measuring the behavior and the respiration amount of the test organism.

Description

TECHNICAL FIELD [0001] The present invention relates to a container for measuring microbial behavior and respiratory physiology,

The present invention relates to a container for measuring microbial activity and respiratory physiological activity, and more particularly, to a container for measuring microbial behavior and respiratory physiological activity capable of simultaneously measuring microbial behavior and oxygen consumption.

In recent years, interest in photosynthetic microorganisms and microorganisms has increased due to functional diversity, and the scope of the research has expanded in various fields. Microbial organisms have recently been used for biological studies due to environmental problems such as marine pollution, ocean acidification, and global warming.

As a means of studying these microorganisms, the behavior tracking of the test organisms is a method of analyzing the movement and distance of an individual within a certain space, and analyzing the developed program by applying various variables such as moving distance and speed .

The respiration measuring apparatus for measuring the respiration amount of living organisms in the water includes a respiration measurement container for containing the test organism to be measured on a circulating route circulating with water filled therein and one or two oxygen sensors on the circulation route So that the amount of oxygen consumed by the test organism is indirectly measured.

The behavioral and breathing measurement vessels of the microorganisms so far produced have been separately manufactured and used.

In other words, conventional measurement vessels used to track the behavior of living creatures have difficulty in extracting the behavioral variables of the test organisms when the inner shape of the tank is round or curved, and since there is no cover part in most cases, And a case of separation from the water tank was issued.

On the other hand, the water tank with the cover has the problem that if the surface of the lid is bent, the size and shape of the creature frequently changes, so that it is wrong to identify the same object in the process of tracking the subject.

Conventional breathing measurement vessels used in breathing apparatuses can be divided into a form in which the interior is exposed to the atmosphere and a completely sealed form as disclosed in Korean Registered Utility Model No. 20-0280723 (published on Jul. 13, 2002) In the former case, it was impossible to accurately measure the amount of dissolved oxygen due to atmospheric exposure. In the latter case, although the production cost is low, since the circulating water inlet and outlet were made of hard material, the breakage was frequently issued, And a sealing member for sealing the measurement container must be separately manufactured.

Registered Utility Model No. 20-0280723 of Korea Registered Date: July 13, 2002

It is an object of the present invention to provide a method and apparatus for monitoring the behavior of an experiment organism including a microorganism and at the same time, it is possible to completely block the contact with the atmosphere when measuring the amount of respiration, And to provide a container for measuring microbial activity and respiratory physiological activity.

The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to at least partially solve the problems in the conventional arts. It can be understood.

According to the present invention, the above object is achieved by a method for measuring and observing microbial activity and respiratory physiological activity according to the present invention, comprising the steps of: forming an accommodation space having an opening upward, And a discharge port for discharging the circulating water, wherein the bottom of the receiving space is sealed with a bottom member made of a transparent material, An upper cover of a transparent material which is hermetically coupled to an upper portion of the biological storage member such that the accommodation space is blocked from the atmosphere; First and second valve devices respectively installed in the inlet and the outlet to control the circulating water flowing into the receiving space of the biological storage member and connected to the circulation loop of the respiratory physiological activity measuring device; And a lighting device for blocking scattering of external light flowing into the accommodation space and irradiating light to the bottom member for identification of the living creatures, .

The first and second valve devices may be provided with adjusting handles for adjusting inflow and outflow amount of the circulating water and may be configured to be detachable and attachable to the inlet and outlet by the screw fastening structure.

A sealing member for hermetic sealing may be provided between the biological storage member and the upper cover.

The bottom member, the biological storage member, the sealing member, and the top cover may be integrally combined by a plurality of long bolts and nuts.

The inlet is provided at a position higher than the outlet so that the circulating water flowing into the receiving space is discharged to the outlet by the head difference, and the inlet and the outlet may be arranged in the diagonal direction, respectively.

The lighting device includes: a body that accommodates a plurality of LEDs or bulbs and has an on / off switch; And a light diffusing plate coupled to the body to uniformly diffuse the light generated from the LED or the bulb and to irradiate the light into the receiving space.

The light diffusing plate may be made of white.

According to the present invention, since the bottom member and the top cover of the biological storage member are made of a transparent material and the illuminating device provided with the white light diffusing plate is provided on the bottom surface of the bottom member, tracking of the object of the camera can be smoothly performed, The space is sealed by the bottom member and the upper cover, thereby preventing the oxygen from the atmosphere from entering the receiving space, allowing the microorganisms to easily enter and exit, and circulating the water smoothly, It is possible to provide an effect of simultaneously measuring the breath volume.

In addition, since the first and second valve devices are installed on the circulation water inlet and the discharge port, respectively, so that the circulation water can be prevented from being leaked, and the valve device can be easily assembled .

1 is an exploded perspective view showing a container for measuring microbial activity and respiratory physiological activity according to the present invention.
FIG. 2 is a perspective view of the combined state of the microbial behavior and respiratory physiological activity measurement container shown in FIG. 1.
3 is a cross-sectional view of the combined state of the microbial behavior and respiratory physiological activity measurement container shown in FIG.
FIG. 4 is a plan view showing the microbial activity and respiratory physiological activity measurement container shown in FIG. 2. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, the well-known functions or constructions are not described in order to simplify the gist of the present invention.

1 is an exploded perspective view showing a container for measuring microbial activity and respiratory physiological activity according to the present invention, and FIG. 2 is a perspective view showing a combined state of the microbial behavior and respiratory physiological activity measurement container shown in FIG. 1 And FIG. 3 is a sectional view of the combined state of the microbial behavior and respiratory physiological activity measurement container shown in FIG. And FIG. 4 is a plan view showing the microbial behavior and respiratory physiological activity measurement container shown in FIG.

A biological storage member (20) having an accommodation space (S) opened in an upward and downward direction, and an upper part of the accommodation space (S) A bottom member 30 coupled to the lower end of the biological storage member 250 to cover the lower portion of the accommodation space S, A first and a second valve devices 50A and 50B respectively coupled to an inlet 22 and an outlet 24 provided on both sides of the biological storage member 20; And a lighting device 80 disposed at the bottom of the bottom member 30 for irradiation.

This will be described more specifically.

The biological storage member 20 has a rectangular box shape in which an accommodation space S opened upwardly and downwardly is formed therein. An inlet 22 for introducing the circulating water into the accommodation space S is formed at an upper side of one side thereof And a discharge port 24 for discharging the circulating water of the accommodation space S is provided at a position lower than the inflow port 22 on the side of the opposite direction. The discharge port 24 is provided lower than the inlet port 22 in order to allow the circulation water flowing into the inlet port 22 to be easily discharged by the head difference.

The inlet (22) and the outlet (24) are arranged diagonally to each other. This is to allow the inflowed circulation water to circulate in the accommodation space S without being discharged immediately, and to stay for a while.

A bottom member 30 made of a transparent material is coupled to the lower end of the bio-storage member 20. [ Therefore, the lower part of the accommodation space S can be covered by the bottom member 30 made of a transparent material.

At this time, the biological storage member 20 may be formed in a structure in which the accommodation space S is opened only upward, and the bottom may be made of a transparent material.

The bottom member 30 is made of a transparent material so that the microorganisms contained in the accommodation space S can be easily observed.

The upper cover 40 is hermetically coupled to the upper part of the biological storage member 20 so that the receiving space S is shut off from the atmosphere. .

A sealing member 70 is provided between the upper end of the bio storey member 20 and the upper lid 40 so as to keep the airtight and prevent the outside air from flowing into the receiving space S. [ Of course, a sealing member 70 is also provided between the lower end of the biological storage member 20 and the bottom member 30 to maintain airtightness.

The bottom member 30 and the biofilm storage member 20, the sealing member 70 and the top cover 40 are sequentially arranged and then combined by a plurality of long bolts 90A and a nut 90B to be integrated. Since the flat washer 90C is provided between the nut 90B and the upper cover 40, when the nut 90B is tightened, the bottom member 30, the sealing member 70, The sealing member 70 and the upper lid 40 can be firmly combined and integrated so that the individual components are combined and integrated by the plurality of long bolts 90A and the nuts 90B, S can be improved. It is a matter of course that a sealing member is provided between the bottom member 30 and the biological storage member 20 when the bottom member 30 and the biological storage member 20 are separately manufactured.

The first and second valve devices 50A and 50B are provided for controlling the circulating water flowing into the accommodation space S of the biohazard member 20 and circulating through the inlet 22 and the outlet 24, do. The first and second valve devices 50A and 50B are connected to the circulation loop 10 of the respiratory physiological activity measuring device (not shown).

The first and second valve devices 50A and 50B are provided with a control handle 52 for controlling the inflow and outflow amount of the circulating water and are detachably mounted on the inflow port 22 and the discharge port 24 by the screw fastening structure. . That is, each of the first and second valve devices 50A and 50B having the adjustment handle 52 is coupled to the inlet 22 and the outlet 24 by a screw fastening structure. Therefore, the first and second valve devices 50A and 50B can be easily removed and mounted.

The illumination device 80 includes a bottom member 30 which is transparent to irradiate light in order to block scattering of external light introduced into the accommodation space S and to facilitate identification of an experiment organism (including microbes) .

The illumination device 80 includes a body 82 having a plurality of LEDs or bulbs and provided with an on / off switch, and a light source 86 composed of an LED or a bulb, And a light diffusing plate 84 coupled to the body 82 to illuminate the interior of the body. Here, the light diffusion plate 84 is preferably made of white. This is to facilitate identification of microorganisms.

As shown in FIG. 3, a light diffusion plate 84 is provided to allow light to be uniformly transmitted to the bottom member 30.

Such a lighting device 80 may be a surface-emitting backlight device.

In order to observe and measure the behavior and respiratory physiological activity of microorganisms using the microbial behavior and respiratory physiological activity container constructed as described above, the circulation loop 10 of the respiratory physiological activity measuring device and the first and second valve devices 50A , And 50B, respectively.

At this time, the bottom member 30, the biofilm storage member 20, the sealing member 70 and the top lid 40 are sequentially arranged and then combined by a plurality of long bolts 90A and a nut 90B, The airtightness of the accommodation space S is improved so that the circulation water does not leak or the outside air does not flow into the accommodation space S.

After the first and second valve devices 50A and 50B are opened, the circulating water is supplied to the accommodation space S.

The circulation water is supplied to the accommodation space S and the illumination device 80 is turned on so that the light of the illumination device 80 is uniformly projected onto the bottom member 30 made of transparent material through the optical diffusion plate 84 .

Therefore, the observer can easily observe the microorganisms housed in the receiving space S outside the transparent upper cover 40, and can photograph the camera through the upper lid 40. [

Particularly, since the light of the illumination device 80 is uniformly irradiated to the bottom member 30 through the white light diffuser plate 84, scattering due to external light can be prevented, and the microorganisms can be more accurately and clearly So that the activity of the microorganism can be more easily observed, and a clear picture can be obtained.

On the other hand, the circulating water flowing into the receiving space S through the inlet 22 through the opening of the first valve device 50A is discharged through the outlet 24 after staying in the receiving space S for a while. At this time, the circulation (discharge) of the circulating water can be controlled by opening and closing the first and second valve devices 50A and 50B.

Such a microbial activity and respiratory physiological activity measurement container is composed of a plurality of long bolts 90A after the bottom member 30, the biological storage member 20, the sealing member 70 and the top lid 40 are sequentially disposed, The airtightness of the accommodation space S can be improved and the airtightness can be completely cut off and the circulation water can be leaked or the external air can not flow into the accommodation space S, Since the first and second valve devices 50A and 50B are installed in the inlet 22 and the outlet 24, not only biological entry and exit can be facilitated, but also circulation water is smoothly circulated and organisms are observed for a long time It will be possible.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It is obvious to those who have. Accordingly, it should be understood that such modifications or alterations should not be understood individually from the technical spirit and viewpoint of the present invention, and that modified embodiments fall within the scope of the claims of the present invention.

10: circulation loop 20: biological storage member
22: inlet 24: outlet
30: bottom member 40: lid member
50A: first valve device 50B: second valve device
70: sealing member 80: illuminating device
82: body 84: light diffusing plate
86: Light source 90A: Long bolt
90B: Nut 90C: Flat washer

Claims (7)

For measuring and observing microbial behavior and respiratory physiological activity,
A biological storage member in which an accommodating space having an opening upward is formed and has an inlet for introducing the circulating water into the accommodating space and a discharge port for discharging the circulating water of the accommodating space, And a top cover made of a transparent material that is hermetically coupled to the upper portion of the biological storage member,
Wherein the inlet port is provided at a position higher than the outlet so that the circulating water flowing into the accommodation space is discharged to the outlet by the head difference, and the inlet port and the outlet port are closed by the circulation water flowing into the accommodation space, Are arranged in diagonal directions with respect to each other so as to be circulated,
Wherein the inlet and outlet are provided with first and second valve devices connected to the circulation loop of the respiratory physiological activity measuring device to control the circulating water flowing into the accommodating space of the biological storage member and circulated, The valve device is provided with a control handle for controlling the inflow amount and the discharge amount of the circulating water and is installed so as to be detachable and attachable to the inflow port and the discharge port by a screw fastening structure,
A bottom member of a transparent material is hermetically coupled to a lower portion of the accommodation space and a bottom surface of the biological storage member is shielded from scattering of external light flowing into the accommodation space, The lighting apparatus includes a body accommodating a light source including a plurality of LEDs or bulbs, and having an on / off switch; And a light diffusion plate coupled to the body for uniformly diffusing the light generated from the light source and irradiating the light to the inside of the accommodation space through the bottom member,
Characterized in that the light diffusing plate is made of white.
Microbial action and respiratory physiological activity measurement vessel. delete The method according to claim 1,
Characterized in that a sealing member for sealing is provided between the biological storage member and the top cover.
Microbial action and respiratory physiological activity measurement vessel. The method of claim 3,
Characterized in that the bottom member, the biological storage member, the sealing member, and the top cover are combined by a plurality of long bolts and nuts,
Microbial action and respiratory physiological activity measurement vessel.


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